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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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16-08-2012 дата публикации

Device and method for particle complex handling

Номер: US20120208716A1
Автор: David J. Liu, Kai Wu, Kenneth B. Swartz, Mineo Yamakawa, Xing Su
Принадлежит: Intel Corp

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

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Номер записи: 1
16-01-2014 дата публикации

Hydrogen sulphide sampling method

Номер: US20140017144A1
Автор: Daniel Dessort, Nadine Loubere, Robert Le Van Loi
Принадлежит: TOTAL SE

A method for sampling a sulphur-containing solid product including supplying a gas flow comprising hydrogen sulphide, bringing the gas flow into contact with a solid reagent and reacting the solid reagent with the hydrogen sulphide contained in the gas flow, the reaction fixing the sulphur of the hydrogen sulphide by forming a sulphur-containing solid product which is different in colour from the solid reagent, and recovering the sulphur-containing solid product. The invention also relates to a device suitable for the implementation of this method.

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Номер записи: 2
04-01-2018 дата публикации

CONTINUOUS DIFFUSION DENUDING WITH MOVING DENUDING SURFACE

Номер: US20180001260A1
Автор: Cooper John Arthur
Принадлежит: Cooper Environmental Services LLC

A duct can be configured to receive a denuding gas flow. A solid denuding surface that is connected to a drive system can be configured to move the solid denuding surface within the duct while the solid denuding surface is continuously concentrating one or more gas-phase species from the denuding gas flow on the denuding surface. Also, a denuding gas flow can be passed along a denuding surface to concentrate one or more gas phase species from the denuding gas flow onto the denuding surface with a diffusion denuding action. The denuding surface can be moved while continuing to concentrate the one or more gas phase species from the denuding gas flow onto the denuding surface. 1. A denuding apparatus comprising:an exposure zone configured to receive a denuding gas;a solid denuding surface positioned at least partially within the exposure zone and configured to concentrate a gas-phase species from the denuding gas on the denuding surface;a drive system connected to the solid denuding surface, the drive system being configured to move the solid denuding surface relative to the exposure zone while the solid denuding surface is concentrating the gas-phase species from the denuding gas on the denuding surface; anda sensor configured to sense quantities of the gas-phase species concentrated on the denuding surface while the drive system moves the solid denuding surface relative to the exposure zone and while the solid denuding surface is concentrating the gas-phase species from the denuding gas on the denuding surface.2. The apparatus of claim 1 , wherein the denuding surface comprises a continuous surface and the apparatus further comprises a treatment zone through which the solid denuding surface is configured to pass to refresh one or more denuding properties of the solid denuding surface.3. The apparatus of claim 1 , wherein the sensor is further configured to quantify quantities of the gas-phase species.4. The apparatus of claim 3 , further comprising a data processing ...

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Номер записи: 3
04-01-2018 дата публикации

CELL SEPARATION APPARATUS

Номер: US20180002666A1
Автор: Nelson Josh, Williams Stuart K., WOLTERS Rolf, Yang Anthony
Принадлежит: TISSUE GENESIS, LLC

Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target. 1. A system for obtaining a target cell volume , comprising:a first chamber comprising a first separation mechanism and configured to separate a volume of biological material into a first retained volume and a first transfer volume; receive the first transfer volume from the first chamber and', 'separate, in response to receiving the first transfer volume, a second transfer volume and a second retention volume from the first transfer volume;, 'a second chamber in fluid communication with the first chamber and configured to receive the second transfer volume; and', 'separate a third transfer volume and a waste volume from the second transfer volume;, 'a third chamber comprising a first side and a second side, wherein the first side is in fluid communication with the second chamber and configured toa pump in fluid communication with at least the third chamber, wherein the pump is configured in a first state to establish a horizontal flow of the second transfer volume from the first side of the third chamber to the second side of the third chamber;a waste collection repository in fluid communication with the second side of the third chamber via a first coupling and configured to receive the waste volume; anda product collection repository in fluid communication with the second side of the third chamber via a second coupling and configured to receive the third transfer volume, wherein the third transfer volume comprises a predetermined volume of cells and fluid.2. The system of claim 1 , wherein the first separation mechanism comprises a ...

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Номер записи: 4
02-01-2020 дата публикации

GENERIC PRETREATMENT REAGENTS FOR ANALYTE DETERMINATIONS

Номер: US20200003666A1
Автор: Bylda Caroline, Fischer Thomas, Hegel Ewelina, Josel Hans-Peter, Lopez-Calle Eloisa, Roedl Josef
Принадлежит: ROCHE DIAGNOSTICS OPERATIONS, INC.

The present disclosure relates to an in vitro method for releasing analytes from a sample involving contacting the sample with (i) a chaotropic agent, (ii) an organic solvent, (iii) a detergent, and (iv) at least one agent providing bicarbonate ions. The present disclosure further relates to a release agent for releasing analytes from a sample having the aforesaid compounds. Moreover, the present disclosure relates to uses, kits, methods and devices related to the method and the release agent of the present disclosure. 1. An in vitro method for releasing analytes from a sample comprising contacting said sample with (i) a chaotropic agent , (ii) an organic solvent , (iii) a detergent , and (iv) at least one agent providing bicarbonate ions.2. The method of claim 1 , wherein the concentration of said chaotropic agent is of from 1 M to 4 M;wherein the concentration of said organic solvent is of from 2% (v/v) to 20% (v/v);wherein the concentration of said detergent is of from 0.2% (w/v) to 20% (w/v); andwherein the concentration of bicarbonate ions formally provided by said at least one agent providing bicarbonate ions is of from 0.1 M to 2 M.3. The method of claim 1 , wherein said chaotropic agent is guanidine hydrochloride and/or urea;wherein said organic solvent is acetonitrile;wherein said detergent is a non-ionic detergent; andwherein said at least one agent providing bicarbonate ions comprises a cyclic carbonate ester.4. The method of claim 1 , wherein said sample is a bodily fluid sample.5. The method of claim 1 , wherein said analytes comprise at least one of a steroid hormone claim 1 , an oligopeptide claim 1 , a polyketide claim 1 , and a vitamin.6. The method of claim 1 , wherein said steroid hormone is at least one of an androgen claim 1 , an estrogen claim 1 , and a progestogen;wherein said oligopeptide is a cyclic oligopeptide antibiotic;wherein said polyketide is a macrolide, tacrolimus, sirolimus, or everolimus; andwherein said vitamin is a vitamin D.7. ...

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Номер записи: 5
02-01-2020 дата публикации

TECHNIQUES FOR TOXIC METAL DETECTION AND SPECIATION IN AQUEOUS MATRICES

Номер: US20200003745A1
Автор: Dozortsev Vladimir, Saini Harmesh K.
Принадлежит:

An in-situ measurement apparatus automatically draws aqueous samples on an intermittent or ad-hoc basis and measures specific metal specie concentration. The apparatus can perform both raw measurement of specific metal specie, as well as processing to convert other species of the same metal to the specific metal specie or to destroy or remove unwanted masking agents (e.g. organics). In one application, “dirty” water from a scrubber is measured for Se(IV) presence (using a renewable voltametric system), both with and without the masking agents present; in addition, selective processing converts other selenium species to Se(IV), permitting assessment of total selenium and measurement of Se(VI) presence. Automated reactions can then be taken to remove detected toxic substances from waste water without excess reliance on treatment chemicals, and so as to ensure that only water complaint with regulatory standards is released into the environment. 1. An apparatus for the measurement of metal concentration in an aqueous matrix , the apparatus comprising:an extraction mechanism to draw samples of the aqueous matrix from a supply;a measurement device to measure concentration of a specific chemical specie of a predetermined metal;a subsystem to process at least one of the samples using predetermined processing, the predetermined processing including at least one of chemical processing, heat processing or ultraviolet light exposure, to create one or more processed samples;a transfer mechanism to transfer samples to the measurement device for measurement of the concentration of the specific chemical specie of the predetermined metal, including at least one of the one or more processed samples, and a sample drawn from the supply which has not been subjected to the predetermined processing; anda control system;wherein the predetermined processing further comprises at least one of removal of an organic material from one of the samples drawn from the supply, or conversion of a ...

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Номер записи: 6
03-01-2019 дата публикации

Enzymatic sample purification

Номер: US20190003937A1
Автор: Anita Rogacs, Viktor Shkolnikov
Принадлежит: Hewlett Packard Development Co LP

An enzymatic purification method involves the introduction of a sample comprising a target analyte and amino acids into a porous matrix of a reaction chamber. The reaction chamber includes first pores and second pores. The first pores contain polypeptide synthesis enzymes that react with the amino acids to form polypeptides. First pores having a first size to be accessible by amino acids but inaccessible by the subsequently formed polypeptides. The second pores have a second size greater than the first size, are in contact with the first pores and form a series extending from within the reaction chamber to a waste chamber. The formed polypeptides are migrated through the series of second pores to the waste chamber. The target analyte of the sample is extracted from the reaction chamber.

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Номер записи: 7
20-01-2022 дата публикации

SERINE HYDROLASE PROFILING ASSAY IN BIOTHERAPEUTICS

Номер: US20220018849A1
Автор: Adam Gregory C., II Douglas D., Letarte Simon, Li Xuanwen, RICHARDSON
Принадлежит:

The present disclosure describes a method of identifying serine hydrolase in a biological test sample obtained from protein production with a fluorophosphonate-containing probe. The present disclosure also provides a method of identifying one or more serine hydrolases in the biological test sample as causing PS-80 or PS-20 degradation. 1. A method of identifying serine hydrolase in a biological test sample obtained from recombinant protein production , comprising the steps of:a. adding a fluorophosphonate probe to the biological test sample comprising a serine hydrolase in conditions that allow the probe to bind to the serine hydrolase;b. removing the free fluorophosphonate probes not bound to the serine hydrolase;c. enriching the probe-bound serine hydrolases with an agent that binds to the probes;d. digesting the serine hydrolase; ande. identifying the serine hydrolase in the test sample with mass spectroscopy.2. A method of identifying one or more serine hydrolases in a biological test sample causing PS-80 or PS-20 degradation , wherein the biological test sample is obtained from recombinant protein production , comprising the steps of:a. adding a fluorophosphonate probe to the biological test sample that exhibits PS-80 or PS-20 degradation in conditions that allow the probe to bind to the serine hydrolase;b. removing the free fluorophosphonate probes not bound to the serine hydrolase;c. enriching the probe-bound serine hydrolases with an agent that binds to the probes;d. digesting the serine hydrolase; ande. identifying the one or more serine hydrolases in the test sample with mass spectroscopy.3. The method of or , wherein the agent in step c) is strepavidin or avidin beads.4. The method of or , wherein in step d) tyrpsin is used.5. The method of claim 1 , wherein the biological test sample is obtained from a CHO cell harvest cell culture fluid for antibody production claim 1 , and optionally purified from one or more affinity claim 1 , ion exchange claim 1 , ...

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Номер записи: 8
10-01-2019 дата публикации

Method for Recovering Metal and Method for Analyzing Metal

Номер: US20190011428A1
Автор: Kudo Akitsugu, Yamada Hirofumi
Принадлежит: ARKRAY, INC.

A method for recovering a metal that uses a reduced amount of a chelating agent is described, where the method includes a complex forming step of forming, in a mixture, a complex between a metal in a sample and a chelating agent; a complex depositing step of depositing the complex in the mixture; and a metal recovering step of recovering the deposited complex from the mixture, thereby recovering the metal in the sample. 1. A method for recovering a metal , the method comprising:mixing a sample with a chelate solution containing a chelating agent dissolved therein to form a mixture such that the pH of the mixture becomes a pH at which the chelating agent is insoluble, thereby forming and depositing a complex containing a metal in the sample and the chelating agent; andrecovering the deposited complex from the mixture.2. The method according to claim 1 , wherein the chelate solution is obtained by dissolving the chelating agent in an aqueous solvent.3. The method according to claim 1 , wherein the chelate solution is obtained by dissolving the chelating agent in an amphipathic organic solvent.48-. (canceled)9. The method according to claim 1 , wherein the pH of the sample is adjusted to an insoluble pH at which the chelating agent is insoluble.10. (canceled)11. The method according to claim 1 , wherein in the recovering claim 1 , the mixture is filtrated to recover the deposited complex.12. The method according to claim 1 , wherein the sample is urine.13. The method according to claim 1 , wherein the metal is at least one selected from the group consisting of Bi claim 1 , Hg claim 1 , Cd claim 1 , Pd claim 1 , Zn claim 1 , Tl claim 1 , Ag claim 1 , Pb claim 1 , As claim 1 , and Al.14. The method according to claim 1 , wherein the chelating agent contains a sulfur-containing group.15. The method according to claim 1 , wherein the chelating agent is 1 claim 1 ,5-diphenyl-3-thiocarbazone.16. The method according to claim 1 , wherein in the mixing claim 1 , the mixture ...

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Номер записи: 9
21-01-2016 дата публикации

LIQUID TISSUE PREPARATION FROM HISTOPATHOLOGICALLY PROCESSED BIOLOGICALLY SAMPLES, TISSUES AND CELLS

Номер: US20160018304A1
Автор: Darfler Marlene M., KRIZMAN David B.
Принадлежит:

The current invention provides a method for directly converting histopathologically processed biological samples, tissues, and cells into a multiuse biomolecule lysate. This method allows for simultaneous extraction, isolation, solubilization, and storage of all biomolecules contained within the histopathologically processed biological sample, thereby forming a representative library of said sample. This multi-use biomolecule lysate is dilutable, soluble, capable of being fractionated, and used in any number of subsequent experiments.

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Номер записи: 10
18-01-2018 дата публикации

SYSTEM AND METHOD FOR SAMPLING HALOSILANES

Номер: US20180017472A1
Автор: Wiederin Daniel R.
Принадлежит:

This disclosure is directed to a system and method relevant to sampling halosilanes or other water-reactive samples. In embodiments, a system for hydrolyzing samples includes a container with a receiving liquid (e.g., an HF solution) contained therein and an actuator coupled with the container. The actuator can be configured to rotate the container, thereby inducing a vortex in the receiving liquid. The system further includes a sample tube configured to direct a halosilane sample into the vortexed receiving liquid. The sample tube can be oriented to release the sample in a flow direction of the vortexed receiving liquid. 1. A system for hydrolyzing samples , comprising:a container with a receiving liquid contained therein;an actuator coupled with the container, the actuator being configured to rotate the container, thereby inducing a vortex in the receiving liquid; anda sample tube configured to direct a sample into the vortexed receiving liquid, wherein the sample tube is oriented to release the sample in a flow direction of the vortexed receiving liquid.2. The system of claim 1 , wherein the sample comprises a halosilane.3. The system of claim 1 , wherein the receiving liquid comprises a hydrofluoric acid solution.4. The system of claim 1 , wherein the sample tube is configured to direct an inert gas into the vortexed receiving liquid prior to introduction of the sample.5. The system of claim 1 , wherein an end of the sample tube that directs the sample into the vortexed receiving liquid is tapered or coupled to a nozzle.6. The system of claim 1 , further comprising a second tube configured to direct an evaporation gas into the container.7. The system of claim 6 , wherein the evaporation gas comprises nitrogen.8. The system of claim 6 , wherein the evaporation gas is heated above an ambient temperature.9. The system of claim 6 , wherein the sample tube and the second tube are parallel to one another.10. The system of claim 6 , wherein the sample tube and the ...

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Номер записи: 11
18-01-2018 дата публикации

Methods of analyzing carbon nanostructures, methods of preparation of analytes from carbon nanostructures, and systems for analyzing carbon nanostructures

Номер: US20180017473A1
Автор: Bashir Hussein WARSAMA, Filipa Fernandes SIMOES, Pedro Miguel Ferreira Joaquim DA COSTA, Shashikant Pandurang PATOLE, Tahir YAPICI
Принадлежит: King Abdullah University of Science and Technology KAUST

Provided herein is a method determining the concentration of impurities in a carbon material, comprising: mixing a flux and a carbon material to form a mixture, wherein the carbon material is selected from the group consisting of graphene, carbon nanotubes, fullerene, carbon onions, graphite, carbon fibers, and a combination thereof; heating the mixture using microwave energy to form fused materials; dissolution of the fused materials in an acid mixture; and measuring the concentration of one or more impurities.

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Номер записи: 12
21-01-2021 дата публикации

TEST DEVICE AND METHOD FOR THE SEMI-QUANTITATIVE DETERMINATION OF CHLORINE DIOXIDE IN A LIQUID SAMPLE CONTAINING FREE CHLORINE

Номер: US20210018478A1
Автор: HOFFMANN Jürgen, HUSMANN Ralph, Lange Dominik, SCHILLBERG Tobias
Принадлежит:

Test device and method for the semi-quantitative determination of chlorine dioxide in a liquid sample has at least two carrier matrices. The first carrier matrix comprises at least one amino acid, sodium thiosulphate, at least one redox indicator, buffer substances and at least one surfactant. The second carrier matrix comprises at least one inorganic iodide salt, starch and/or at least one starch derivative, sodium thiosulfate, buffer substances and at least one surfactant. 112323. Test device () for the semi-quantitative determination of chlorine dioxide in a liquid sample , wherein the test device has at least two carrier matrices ( , ) , wherein the first carrier matrix () comprises at least one amino acid , sodium thiosulphate , at least one redox indicator , buffer substances and at least one surfactant , and wherein the second carrier matrix () comprises at least one inorganic iodide salt , starch and/or at least one starch derivative , sodium thiosulfate , buffer substances and at least one surfactant.21. The test device () according to claim 1 , wherein the amino acid is selected from the group consisting of alanine claim 1 , glycine claim 1 , histidine claim 1 , leucine claim 1 , isoleucine claim 1 , lysine claim 1 , valine and proline claim 1 , wherein glycine is preferred.31. The test device () according to claim 1 , wherein the redox indicator is selected from the group consisting of benzidine claim 1 , o-tolidine claim 1 , o-dianisidine claim 1 , 3 claim 1 ,3′-5 claim 1 ,5′-tetramethylbenzidine and syringaldazine claim 1 , wherein 3 claim 1 ,3′-5 claim 1 ,5′-tetramethylbenzidine is preferred.41. The test device () according to claim 1 , wherein the buffer substances are selected from the group consisting of phosphates claim 1 , acetates claim 1 , citrates claim 1 , tartrates and borates.51. The test device () according to claim 1 , wherein the surfactant is selected from the group consisting of fatty alcohol ethoxylates claim 1 , fatty alcohol ...

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Номер записи: 13
25-01-2018 дата публикации

An Extraction Reagent of Immunosuppressant Drug for Immunoassays

Номер: US20180024124A1
Автор: Nie Yongyan, Shi Xiaojuan, Wu Fengbo
Принадлежит:

The disclosure provides a reagent for extracting immunosuppressant drugs from whole blood sample for immunoassay. The extraction reagent comprises protein denaturant, proteolytic enzyme, surfactant and pH buffer. The disclosure also provides a method and an immunoassay kit for detection of the immunosuppressant concentration in a whole blood sample using the extraction reagent. The extraction reagent of the present disclosure doesn't need the use of organic solvent as that in the traditional extraction method, therefore the adverse effects of the organic solvent on the antibody activity in a detection system and the other relative defects associated to its use are obviated. The drug extraction process of the present disclosure doesn't need centrifugation, the processed sample can be directly applied for immunoassay. The operation for drug extraction by the present disclosure is simple, and the detection result based on this extraction method is accurate. 1. An extraction reagent of the immunosuppressant drug in blood sample for immunoassay , comprising a protein denaturant , proteolytic enzyme , surfactant and pH buffer.2. The extraction reagent according to claim 1 , wherein the protein denaturant is selected from urea claim 1 , guanidine hydrochloride or other non-organic solvent based denaturants.3. The extraction reagent according to claim 2 , wherein the molar concentration of the urea in the extraction reagent is 4 mol/L to 12 mol/L; and alternatively the molar concentration of the guanidine hydrochloride in the extraction reagent is about 1 mol/L to 8 mol/L.4. The extraction reagent according to claim 3 , wherein the molar concentration of the urea in the extraction reagent is 6 mol/L to 8 mol/L; and alternatively the molar concentration of the guanidine hydrochloride in the extraction reagent is 2 mol/L to 6 mol/L.5. The extraction reagent according to claim 1 , wherein the proteolytic enzyme is selected from one or more of subtilisin claim 1 , protease K ...

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Номер записи: 14
17-02-2022 дата публикации

METHOD, SYSTEM AND APPARATUS FOR BLOOD PROCESSING UNIT

Номер: US20220049321A1
Автор: Davidson John F., Richards David, Xue Zheng
Принадлежит: Tangen Biosciences, Inc.

The disclosed embodiments may be used, among others, to extract particles from blood. The particles may include pathogens, viruses, bacteria and other microorganisms present in mammalian blood. An embodiment of the disclosure relates to a system to detect one or more blood-borne pathogens. The exemplary system includes: a transfer assembly having a tube and a hallow needle, the hallow needle centrally located within the transfer assembly tube and configured to communicate a sample material therethrough; a lysing syringe to couple to the transfer assembly, the lysing syringe comprising one or more lysing reagent and a plunger activatable to receive the sample material through the transfer assembly; and a large volume concentrator (LVC) to sealingly couple to the lysing syringe and to separate at least one pathogen from the sample material, the LVC further comprising: a filter support, a membrane, a retainer and a threaded portion. 1. A system to detect one or more blood-borne pathogens , the system comprising:a transfer assembly having a tube and a hallow needle, the hallow needle centrally located within the transfer assembly tube and configured to communicate a sample material therethrough;a lysing syringe to couple to the transfer assembly, the lysing syringe comprising one or more lysing reagent and a plunger activatable to receive the sample material through the transfer assembly; anda large volume concentrator (LVC) to sealingly couple to the lysing syringe and to separate at least one pathogen from the sample material, the LVC further comprising: a filter support, a membrane, a retainer and a threaded portion;wherein the retainer is configured to secure the membrane against the filter support.2. The system of claim 1 , further comprising an adapter to couple to the LVC claim 1 , the adapter configured to allow a twisting motion along a longitudinal axis thereof.3. The system of claim 1 , further comprising an agitator to receive the lysing syringe containing ...

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Номер записи: 15
04-02-2021 дата публикации

BLOOD ANALYZER AND ANALYSIS METHOD

Номер: US20210033592A1
Автор: QI Huan, Ye Bo, YE Yi, ZHENG Wenbo
Принадлежит:

A blood analyzer and a blood analysis method thereof are provided. The blood analyzer includes a test sample preparation apparatus, a flow chamber, a measurement apparatus and a data processor. The method adopts a white blood cell measurement channel to detect a test blood sample subjected to hemolysis and fluorescence staining and utilizes data in a blood ghost region to identify reticulocyte particles, thereby realizing differentiation of reticulocytes and large platelets and realizing finer classification and count of reticulocytes without separately designing an optical detection channel in the blood analyzer. A computer readable storage medium is also disclosed, in which a program is stored, and the program can be executed by the data processor to implement the above method. 137-. (canceled)38. A blood analyzer , comprising:a test sample preparation apparatus for preparing a test sample for measurement, wherein the test sample preparation apparatus at least comprises a reaction cell, and the reaction cell is configured for providing a place for mixing and incubation of a test blood sample, a fluorescence staining agent and a hemolytic agent, and the mixing and incubation causes red blood cells in the test blood sample to be lysed and causes cell particles in the sample to be stained;a flow chamber configured for providing an area for the cell particles in the test sample to pass through one by one and to be irradiated with light;a measurement apparatus comprising a light source and an optical detection device, wherein the light source is configured for emitting a light beam to irradiate the flow chamber, the optical detection device is configured for receiving scattered light and fluorescent light generated by the cell particles under the irradiation of light and outputting scattered light information and fluorescent light information, and the scattered light information at least comprises first angle scattered light information which is used for reflecting ...

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Номер записи: 16
11-02-2016 дата публикации

A METHOD AND A DEVICE FOR ASSESSING WHETHER A DRUG OF ABUSE AND/OR A METABOLITE THEREFOF IS PRESENT IN A KERATIN MATERIAL

Номер: US20160041076A1
Автор: CHERICONI Silvio, GIUSIANI Mario, STEFANELLI Fabio
Принадлежит:

A method and a device, for assessing whether a drug of abuse and/or a metabolite of a drug of abuse is present in a keratin material, where the method includes extracting a drug of abuse and/or a metabolite from a subject's keratin material, by prearranging an amount of the keratin material; prearranging an extraction composition; bringing the keratin material into contact with the extraction composition in order to obtain an extract that contains the drug of abuse and/or the metabolite, wherein the extraction composition contains a compound selected among urea and urea derivatives. 1. A method for assessing whether a drug of abuse and/or a metabolite of a drug of abuse is present in a keratin material , said method comprising: prearranging an amount of said keratin material;', 'prearranging an amount of an extraction composition containing a compound selected from the group consisting of urea and urea derivatives;', 'bringing said amount of said keratin material into contact with said amount of said extraction composition, obtaining an extract containing said drug of abuse and/or said metabolite;, 'a step of extracting said drug of abuse and/or said metabolite from said keratin material, said step of extracting comprising performing a reaction of said drug of abuse and/or said metabolite, which acts as an antigen, with a specific antibody;', 'detecting said antigen-antibody reaction., 'a step of analysing said extract by an antigen-antibody technique comprising2. A method according to claim 1 , wherein said reaction of said drug of abuse and/or of said metabolite with said specific antibody is a first reaction claim 1 , and said method comprises a step of comparing said first reaction with a second reaction between said antibody and a reference antigen for said drug of abuse and/or for said metabolite.3. A method according to claim 2 , wherein said reference antigen is a marked antigen adapted to produce a visually detectable chromophore compound in said second ...

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Номер записи: 17
24-02-2022 дата публикации

METHOD AND PRODUCT FOR PREPARING A PROTEIN-CONTAINING SAMPLE FOR ANALYSIS BY MASS SPECTROMETRY

Номер: US20220057306A1
Автор: Foster Greg A., Krawitzky Michael O., Lopez Ferrer Daniel, Robitaille Aaron M.
Принадлежит:

A method for preparing a protein-containing sample for analysis by mass spectrometry includes introducing the sample into a reaction vessel. The reaction vessel contains a reagent mixture including pre-measured quantities of an immobilized proteolytic enzyme, a reducing agent and an alkylating agent. The contents of the reaction vessel are activated by heating or by sonication. 110.-. (canceled)11. A method for manufacturing a product for use in preparing a protein-containing sample , comprising:providing a closeable reaction vessel having an internal volume; an immobilized proteolytic enzyme;', 'a reducing agent; and', 'an alkylating agent., 'introducing a reagent mixture comprising a predetermined amount of each of the following reagents into the internal volume of the closeable reaction vessel12. The method of claim 11 , wherein the proteolytic enzyme is selected from the group consisting of trypsin claim 11 , LysC claim 11 , LysN claim 11 , AspN claim 11 , GluC claim 11 , ArgC and chymotrypsin.13. The method of claim 11 , further comprising introducing a cell lysing agent into the internal volume of the reaction vessel.14. The method of claim 13 , wherein the cell lysing agent is selected from the group consisting of: a detergent in an amount up to 1% (vol/vol); an organic solvent in an amount up to 10% (vol/vol); and urea up to 1M as calculated after the sample is added.15. The method of claim 11 , further comprising a formulation buffered to store the mixture.16. The method of claim 11 , wherein the reducing agent is tris(2-carboxyethyl) phosphine (TCEP) and the alkylating agent is chloroacetamide (CAA) or Iodacetic acid (IAC) or Iodoacetamide (IAA).17. The method of claim 11 , wherein the mixture further comprises calcium chloride (CaCl).18. The method of claim 11 , wherein the reducing agent is tris(2-carboxyethyl) phosphine (TCEP) at a concentration between 1 mM and 5 mM claim 11 , and wherein the alkylating agent is one of: chloroacetamide (CAA) at a ...

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Номер записи: 18
18-02-2016 дата публикации

CONTINUOUS DIFFUSION DENUDING WITH MOVING DENUDING SURFACE

Номер: US20160045863A1
Автор: Cooper John Arthur
Принадлежит:

A duct can be configured to receive a denuding gas flow. A solid denuding surface that is connected to a drive system can be configured to move the solid denuding surface within the duct while the solid denuding surface is continuously concentrating one or more gas-phase species from the denuding gas flow on the denuding surface. Also, a denuding gas flow can be passed along a denuding surface to concentrate one or more gas phase species from the denuding gas flow onto the denuding surface with a diffusion denuding action. The denuding surface can be moved while continuing to concentrate the one or more gas phase species from the denuding gas flow onto the denuding surface. 1. A denuding apparatus comprising:a duct configured to receive a denuding gas flow;a solid denuding surface positioned at least partially within the duct and configured to concentrate a gas-phase species from the denuding gas flow on the denuding surface;a drive system connected to the solid denuding surface, the drive system being configured to move the solid denuding surface relative to the duct while the solid denuding surface is concentrating the gas-phase species from the denuding gas flow on the denuding surface; anda sensor configured to sense quantities of the gas-phase species concentrated on the denuding surface while the drive system moves the solid denuding surface relative to the duct and while the solid denuding surface is concentrating the gas-phase species from the denuding gas flow on the denuding surface.2. The apparatus of claim 1 , wherein the denuding surface comprises a continuous surface and the apparatus further comprises a treatment zone through which the solid denuding surface is configured to pass to refresh one or more denuding properties of the solid denuding surface.3. The apparatus of claim 1 , wherein the sensor is further configured to quantify quantities of the gas-phase species.4. The apparatus of claim 3 , further comprising a data processing and reporting ...

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Номер записи: 19
03-03-2022 дата публикации

GAS PURIFICATION DEVICE, AND APPARATUS AND METHOD FOR MEASURING NOBLE GAS ISOTOPES

Номер: US20220065760A1
Автор: CAO Chunhui, DU Li, LI Liwu, LI Zhongping, ZHAO Huanhuan
Принадлежит:

Disclosed are a gas purification device, and an apparatus and a method for measuring isotopes of noble gases. The gas purification device includes a housing and a purification mechanism. The housing is provided with a reaction chamber. The reaction chamber is in a vacuum state and is configured to hold a gas sample. The purification mechanism is arranged on the housing and is provided with a cavity. The cavity in the purification mechanism is communicated with the reaction chamber. The purification mechanism is configured to purify the gas sample in the reaction chamber to obtain a purified gas.

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Номер записи: 20
26-02-2015 дата публикации

METHOD AND SYSTEM FOR ACOUSTICALLY TREATING MATERIAL

Номер: US20150056715A1
Автор: He Xiaoyin, JR. James A., Laugharn
Принадлежит: Covaris, Inc.

Methods and systems for acoustically treating material using an acoustic energy system having a movable outer surface that contacts a sample holder. The outer surface may be cylindrical and rotate about a central axis, e.g., so that a sample holder may be driven to move by the outer surface. Acoustic energy may be emitted from within the outer surface to a treatment area outside of, and near, the outer surface. Thus, a sample holder in contact with the outer surface may have a sample exposed to acoustic energy while rotation of the outer surface may move the sample holder relative to treatment area. One or more additional rollers or other components may bias the sample holder into contact with the outer surface, to e.g., so the sample holder is squeezed between the outer surface and a roller or other biasing component. 1. A system for treating a material with acoustic energy , comprising:a coupling medium container that is closed and defines an internal volume, the coupling medium container having an outer surface arranged to rotate about an axis;an acoustic energy source arranged to emit acoustic energy into the internal volume; anda coupling medium located in the internal volume of the coupling medium container, the coupling medium being arranged to transmit acoustic energy from the acoustic energy source to a treatment area outside of the coupling medium container and near the outer to surface;wherein a sample holder is positionable in contact with the outer surface of the coupling medium container and the outer surface is rotatable with movement of the sample holder relative to the treatment area.2. The system of claim 1 , wherein the outer surface is cylindrical and the axis passes through a center longitudinal axis of the cylindrical outer surface.3. The system of claim 2 , wherein the outer surface is rotatable with linear movement of the sample holder relative to the treatment area.4. The system of claim 3 , wherein the outer surface is rotatable with ...

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Номер записи: 21
10-03-2022 дата публикации

ANALYSIS METHOD, ANALYSIS DEVICE AND NON-TRANSITORY COMPUTER READABLE RECORDING MEDIUM STORING

Номер: US20220074832A1
Автор: KUDO Yukihiko
Принадлежит: SHIMADZU CORPORATION

An analysis method is a method of analyzing a second substance in a sample including the second substance produced by decomposition of a first substance, and includes analyzing a sample and a compound having a known concentration and detecting the first substance, the second substance and the above-mentioned compound, calculating an intensity or a concentration of the first substance obtained from the above-mentioned data based on data obtained by the detection and a relative response factor in regard to the first substance and the above-mentioned compound, and producing information about an amount or a concentration of the second substance excluding the second substance produced from the first substance in the analysis, based on an intensity or a concentration of the first substance. 1. An analysis method of analyzing a second substance produced by decomposition of a first substance , including:analyzing a sample and a compound having a known concentration and detecting the first substance, the second substance and the compound;calculating an intensity or a concentration of the first substance based on data obtained by the detection and a relative response factor in regard to the first substance and the compound; andproducing information about an amount or a concentration of the second substance excluding the second substance produced from the first substance in the analysis, based on an intensity or a concentration of the first substance obtained from the data.2. The analysis method according to claim 1 , including acquiring a first threshold value in regard to an intensity or a concentration of the first substance claim 1 , andthe analysis method producing the information based on a concentration of the second substance obtained by the data and whether an intensity or a concentration of the first substance satisfies a first condition based on the first threshold value.3. The analysis method according to claim 2 , including acquiring a second threshold value in ...

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Номер записи: 22
10-03-2022 дата публикации

METHODS OF PREPARING SAMPLES FOR PROTEOMIC ANALYSIS

Номер: US20220074949A1
Автор: Sobansky Matthew R.
Принадлежит:

Provided herein are methods of preparing a protein sample for proteomic analysis. In exemplary embodiments, the method comprises (a) contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent, and (b) isolating a fraction comprising proteins or a source of proteins from the mixture to yield a protein sample or a source of a protein sample, wherein steps of the method are carried out in the absence of exogenous proteolytic enzyme inhibitors, wherein the protein sample is suitable for proteomic analysis. Preferably, the protective agent consists of essentially (i) about 300 g/l to about 700 g/l imidazolidinyl urea; (ii) about 20 g/l to about 60 g/l glycine; and (iii) about 60 g/l to about 100 g/l EDTA; and the protein sample is analysed via mass spectrometry-based proteomic methods. 1. A method of preparing a protein sample for proteomic analysis , comprisinga. contacting a blood sample comprising proteins with a protective agent comprising an anticoagulant (AC) and an aldehyde releaser (AR), to obtain a mixture, optionally, wherein the blood sample is added to a blood collection tube (BCT) comprising the protective agent or the blood sample is directly drawn from a subject into a BCT comprising the protective agent, andb. isolating a fraction comprising proteins from the mixture to yield a protein sample suitable for proteomic analysis,{'claim-text': ['(I) steps (a) and (b) are carried out in the absence of exogenous proteolytic enzyme inhibitors;', '(II) the slope of the best fit line of a line graph of the number of proteins in the protein sample yielded from step (b) plotted as a function of storage time is closer to 0 compared to the slope of the best fit line of a line graph of the number of proteins in a control blood sample not contacted with a protective ...

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Номер записи: 23
21-02-2019 дата публикации

ENZYMATIC SAMPLE PURIFICATION

Номер: US20190056299A1
Автор: Rogacs Anita, Shkolnikov Viktor
Принадлежит: Hewlett-Packard Development

An enzymatic sample purifier comprises a reaction chamber containing an enzyme, a product chamber and a separator. The reaction chamber has a port to receive a sample comprising a protein degradation product. The separator retains polypeptides resulting from reaction of the protein degradation product with the enzyme in the reaction chamber and facilitates transport of purified remaining portions of the sample comprising a target analyte to the product chamber. 1. An apparatus comprising:a reaction chamber containing an enzyme, the reaction chamber having a port to receive a sample comprising a protein degradation product;a product chamber; anda separator to retain polypeptides resulting from reaction of the protein degradation product with the enzyme in the reaction chamber and to transport purified remaining portions of the sample comprising a target analyte to the product chamber.2. The apparatus of further comprising a porous matrix within the reaction chamber immobilizing the enzymes.3. The apparatus of claim 1 , wherein the separator comprises:a porous matrix within the reaction chamber to immobilize the polypeptide; anda transport mechanism to move the purified portions of the sample to the product chamber.4. The apparatus of claim 3 , wherein the transport mechanism is selected from a group of transport mechanisms consisting of an electrophoretic transport and a pressurized liquid flow transport.5. The apparatus of claim 1 , wherein the separator is selected from a group of separators consisting of: a size exclusion chromatography separator claim 1 , a dialysis separator claim 1 , an electro-dialysis separator claim 1 , and an electrophoresis separator.6. The apparatus of further comprising a receiver interconnected to the reaction chamber and the product chamber claim 1 , the receiver sized to removably receive and retain the separator between the reaction chamber and the product chamber.7. The apparatus of further comprising:a first capture moiety in the ...

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Номер записи: 24
20-02-2020 дата публикации

TURBIDIMETER SLUDGE MEASUREMENT

Номер: US20200056989A1
Автор: Bitschnau Hans-Christian, Hahn Harald, Simon Jochen
Принадлежит:

A turbidimeter device, including: a fluidically closed turbidimeter vessel comprising a liquid sample inlet and a liquid sample outlet; a vacuum pump for generating underpressure in the turbidimeter vessel to degas the liquid sample; and an optical measurement device for an optical determination of the liquid sample turbidity. Other aspects are described and claimed. 112.-. (canceled)13. A turbidimeter device , comprising:a fluidically closed turbidimeter vessel comprising a liquid sample inlet and a liquid sample outlet;a vacuum pump for generating underpressure in the turbidimeter vessel to degas the liquid sample; andan optical measurement device for an optical determination of the liquid sample turbidity.14. The turbidimeter device of claim 13 , wherein a sample outlet valve is provided downstream of the liquid sample outlet to fluidically close the liquid sample outlet during the degassing Interval.15. The turbidimeter device of claim 13 , wherein a first flushing opening is provided through which a flushing liquid coming from a flushing liquid source is injected into the turbidimeter vessel.16. The turbidimeter device of claim 13 , wherein a first flushing opening is arranged axially in-line with the optical measurement device.17. The turbidimeter device of claim 13 , wherein a second flushing opening is provided at the top of the turbidimeter vessel.18. The turbidimeter device of claim 13 , wherein a progression evaluation unit is provided for detecting the constancy of the turbidity measurement value of the optical measurement device.19. The turbidimeter device of claim 13 , wherein a filling level detector is provided to detect the liquid filling level of the liquid sample in the turbidimeter vessel.20. The turbidimeter device of claim 13 , wherein the liquid sample inlet is provided at the bottom of the turbidimeter vessel21. A sludge thickening device claim 13 , comprising:a sludge dewatering device for a dewatering sludge, the dewatering device being ...

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Номер записи: 25
20-02-2020 дата публикации

GAS ANALYSIS APPARATUS AND GAS ANALYSIS METHOD

Номер: US20200057024A1
Автор: LACDAN Ma Camille Corrales, Ochiai Ryota, YOSHIMURA Tomoshi
Принадлежит: HORIBA, LTD.

The present invention includes a first flow path through which a sample gas flows, a first analyzer that is provided in the first flow path to measure total hydrocarbon concentration in the sample gas, a second flow path through which the sample gas flows, a non-methane non-ethane cutter that is provided in the second flow path to remove the hydrocarbon components other than the methane and the ethane in the sample gas, a second analyzer that is provided downstream of the non-methane non-ethane cutter in the second flow path to measure the total methane ethane concentration of the methane and the ethane in the sample gas, and a calculation part that calculates the concentration of the hydrocarbon components other than the methane and the ethane in the sample gas with use of the total hydrocarbon concentration by the first analyzer and the total methane ethane concentration by the second analyzer. 1. A gas analysis apparatus comprising:a first flow path through which sample gas flows;a first analyzer that is provided in the first flow path to measure total hydrocarbon concentration in the sample gas;a second flow path through which the sample gas flows;a non-methane non-ethane cutter that is provided in the second flow path to remove hydrocarbon components other than methane and ethane in the sample gas;a second analyzer that is provided downstream of the non-methane non-ethane cutter in the second flow path to measure total methane ethane concentration of the methane and the ethane in the sample gas; anda calculation part that calculates concentration of the hydrocarbon components other than the methane and the ethane in the sample gas with use of the total hydrocarbon concentration by the first analyzer and the total methane ethane concentration by the second analyzer.2. The gas analysis apparatus according to claim 1 , whereinthe second analyzer is calibrated with ethane used and passed through the non-methane non-ethane cutter.3. The gas analysis apparatus ...

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Номер записи: 26
04-03-2021 дата публикации

METHOD FOR AUTOMATIC QUANTITATIVE STATISTICAL DISTRIBUTION CHARACTERIZATION OF DENDRITE STRUCTURES IN A FULL VIEW FIELD OF METAL MATERIALS

Номер: US20210063376A1
Автор: Jia Yunhai, Li Dongling, Li Jie, Shen Xuejing, Wan Weihao, Wang Haizhou, Zhao Lei
Принадлежит:

The invention belongs to the technical field of quantitative statistical distribution analysis for micro-structures of metal materials, and relates to a method for automatic quantitative statistical distribution characterization of dendrite structures in a full view field of metal materials. According to the method based on deep learning in the present invention, dendrite structure feature maps are marked and trained to obtain a corresponding object detection model, so as to carry out automatic identification and marking of dendrite structure centers in a full view field; and in combination with an image processing method, feature parameters in the full view field such as morphology, position, number and spacing of all dendrite structures within a large range are obtained quickly, thereby achieving quantitative statistical distribution characterization of dendrite structures in the metal material. The method is accurate, automatic and efficient, involves a large amount of quantitative statistical distribution information, and is statistically more representative as compared with the traditional measurement of feature sizes of dendrite structures in a single view field. 1. A method for automatic quantitative statistical distribution characterization of dendrite structures in a full view field of metal materials , comprising the following steps:(1) establishment of an object detection model based on deep learningperforming metallographic sample preparation, polishing and chemical corrosion on a standard metal material sample with the same material as a metal material to be detected, so that the surface of the sample shows a clear and complete dendrite structure;acquiring dendrite structure feature maps of the standard metal material sample after the metallographic chemical corrosion by using a fully automatic metallographic microscope, and establishing a dendrite structure feature map data set; labeling images in the dendrite structure feature map data set by using ...

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Номер записи: 27
27-02-2020 дата публикации

Use of Raman Spectroscopy in Downstream Purification

Номер: US20200062802A1
Автор: Cowan Christopher, Passno Christina, Tustian Andrew
Принадлежит:

In situ Raman spectroscopy methods and systems for characterizing or quantifying a protein purification intermediate and/or final concentrated pool during production or manufacture are provided. In one embodiment, in situ Raman spectroscopy is used to characterize or quantify protein purification intermediates critical quality attributes during downstream processing (i.e., after harvest of the protein purification intermediate). For example, the disclosed in situ Raman spectroscopy methods and systems can be used to characterize and quantify protein purification intermediates as the protein purification intermediates are purified, condensed, or otherwise formulated into the final drug product to be sold or administered 1. A method of producing a concentrated protein purification intermediate comprising:determining concentrations of a protein purification intermediate in-real time using in situ Raman spectroscopy while concentrating the protein purification intermediate; andadjusting parameters of the concentrating step in-real time to obtain the concentrated protein purification intermediate and/or final concentrated pool.2. The method of claim 1 , wherein the concentrated protein product has a concentration of 5 mg/mL to 300 mg/mL.3. The method of claim 1 , wherein the concentration of the protein purification intermediate is at least 50 mg/mL.4. The method of claim 1 , wherein the concentration of the protein purification intermediate is at least 150 mg/mL.5. The method of claim 1 , wherein the protein purification intermediate is concentrated using ultrafiltration claim 1 , buffer exchange claim 1 , or both.6. The method of claim 1 , wherein the protein purification intermediate is harvested from a bioreactor claim 1 , a fed-batch culture claim 1 , or a continuous culture.7. The method of claim 1 , wherein determining the concentration of the protein purification intermediate occurs continuously or intermittently in real-time.8. The method of claim 1 , wherein ...

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Номер записи: 28
10-03-2016 дата публикации

Method for Recovering Metal and Kit for Recovery of Metal for Use in the Same

Номер: US20160069854A1
Автор: Shimomura Yuka
Принадлежит: ARKRAY, INC.

A method for recovering a metal, capable of recovering a metal easily without requiring the use of an organic medium, is provided. A first complex between a first chelating agent and a metal present in a sample is formed in a first mixture prepared by mixing the first chelating agent and the sample. Then, the first complex is recovered from the first mixture, and a second complex between the metal derived from the first complex and a second chelating agent is formed in a second mixture prepared by mixing the first complex and an aqueous solution of the second chelating agent. The aqueous solution is under the pH conditions where the first chelating agent can be insoluble in the aqueous solution. Then, a liquid fraction containing the second complex is recovered from the second mixture. Thus, the metal can be recovered. 115.-. (canceled)16. An analysis apparatus for analyzing a metal , comprising:a pH adjusting unit configured to adjust pH;a first mixing unit configured to mix a first chelating agent and a sample comprising a metal;a complex recovering unit configured to recover a first complex between the first chelating agent and the metal in the sample from a first mixture containing the first chelating agent and the sample;a second mixing unit configured to mix an aqueous solution of a second chelating agent and the first complex;a liquid fraction recovering unit configured to recover a liquid fraction containing a second complex between the metal derived from the first complex and the second chelating agent, dissolved therein, from a second mixture of the aqueous solution of the second chelating agent and the first complex;a metal recovering unit configured to recover the metal present in the second complex; andan analyzing unit configured to analyze the recovered metal.17. The analysis apparatus according to claim 16 , wherein the analyzing unit is selected from the group consisting of a GC-ECD (gas chromatography-electron capture detector) claim 16 , a mass ...

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Номер записи: 29
09-03-2017 дата публикации

Device and method for chemical analysis

Номер: US20170067888A1
Автор: Bahram Fotouhi, Edward Alvin Greenfield, Kashayar Javaherian, Mehdi ABEDI, Mohammad E. Taslim, Mohammed FOTOUHI, Reza MOLLAAGHABABA
Принадлежит: Rite Taste LLC

A processing and detection system for detecting presence of at least one gluten protein in a food sample comprises a food processor including: a reservoir containing a process liquid for processing the food sample; a body that comprises a chamber configured to receive the food sample; and a pressing surface configured to press on the reservoir to cause the process liquid to exit the reservoir and mix with the food sample, thereby generating a processed food liquid; and an exit port configured to conduct the processed food liquid out of the food processor; and a cartridge including: at least one sensor configured to receive the processed food liquid and to generate an electrical signal in response to interaction with the at least one gluten protein in the processed food liquid, and an analyzer in electrical communication with the at least one sensor for detecting the electrical signal and determining the presence of the at least one gluten protein in the food sample based on the detected electrical signal.

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Номер записи: 30
11-03-2021 дата публикации

Method for diagnosing tuberculosis

Номер: US20210072235A1
Автор: Eunkyung Kim, Hwa-Jung Kim, Mi-Jin Sohn, Paul Kim, Seungbum YOO, Sunhee Lee
Принадлежит: Sugentech Inc

A method for diagnosing tuberculosis of the present invention can improve the reactivity of tuberculous antigens and detection antibodies by increasing free tuberculous antigens in a sample through a pretreatment step in which the sample of a subject is treated with an acidic material such that the antigens are dissociated from tuberculous antigen complexes, thereby enabling a point-of-care test for tuberculosis and being immediately utilizable as a fast and simple detection method for a point-of-care test.

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Номер записи: 31
09-03-2017 дата публикации

APPARATUS FOR MEASURING IMPURITIES ON WAFER AND METHOD OF MEASURING IMPURITIES ON WAFER

Номер: US20170069515A1
Автор: LEE Seung Wook
Принадлежит:

Provided are an apparatus for measuring impurities on a wafer and a method of measuring impurities on a wafer. The apparatus includes: a wafer aligning device for aligning a wafer; a loading robot for moving and loading the aligned wafer; a rotation stage for rotating the loaded wafer; a scan robot for holding a natural oxide layer etching solution for the wafer and a metallic impurity recovery solution; and a container for receiving a predetermined etching solution and a recovery solution, wherein the scan robot removes an oxide layer on an edge region of the wafer. 1. A method of measuring impurities on a wafer , comprising:aligning a wafer and then loading the wafer on a rotation stage by a loading robot;removing an oxide layer on the edge region of the wafer;collecting metallic impurities on the surface of the wafer edge region having the oxide layer removed, by using a recovery solution; andanalyzing the metallic impurities by using the extracted recovery solution.2. The method according to claim 1 , wherein the removing of the oxide layer on the wafer edge region comprises:approaching the wafer edge region while a scan robot collects and holds an etching solution; androtating the rotation stage having the wafer mounted, while not contacting the wafer edge region and being spaced for a predetermined distance apart from the wafer edge region.3. The method according to claim 1 , wherein an amount (V1) of the etching solution that the scan robot collects and holds is about 100 μL≦V1≦about 1000 μL.4. The method according to claim 1 , wherein the collecting of the metallic impurities on the surface of the wafer edge region having the oxide layer removed comprises:supplying a recovery solution by using the scan robot;contacting the recovery solution with the wafer edge region; andextracting the metallic impurities on the wafer edge region while rotating the rotation stage of the wafer.5. The method according to claim 4 , wherein an amount (V2) of the recovery ...

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Номер записи: 32
16-03-2017 дата публикации

Analysis Pretreatment Device

Номер: US20170072378A1
Автор: MIZUNO Ayako, WU Jiahong, YAMADA Yuji
Принадлежит:

An analysis pretreatment device according to an embodiment includes a chamber capable of containing an analysis object therein. A pressure reducer reduces pressure inside the chamber. An introducing part vaporizes a liquid and introduces the vaporized liquid into the chamber. A first supplier supplies water in a liquid state to the introducing part. A second supplier supplies hydrofluoric acid in a gas state to the introducing part. The introducing part introduces a mixed gas into the chamber. The mixed gas includes vaporized water, which is obtained by vaporizing water in a liquid state, and hydrofluoric acid in a gas state. 1. An analysis pretreatment device comprising:a chamber capable of containing an analysis object therein;a pressure reducer reducing pressure inside the chamber;an introducing part vaporizing a liquid and introducing the vaporized liquid into the chamber;a first supplier supplying water in a liquid state to the introducing part; anda second supplier supplying hydrofluoric acid in a gas state to the introducing part, whereinthe introducing part introduces a mixed gas into the chamber, the mixed gas including a vaporized water, which is obtained by vaporizing water in a liquid state, and hydrofluoric acid in a gas state.2. The device of claim 1 , wherein the analysis object is a nitride film or an oxynitride film provided on a substrate.3. The device of claim 1 , further comprising a collector supplying a chemical liquid onto the substrate after vapor phase decomposition of the analysis object on the substrate claim 1 , and collecting an object to be measured that remains on the substrate into the chemical liquid.4. The device of claim 2 , further comprising a collector supplying a chemical liquid onto the substrate after vapor phase decomposition of the analysis object on the substrate claim 2 , and collecting an object to be measured that remains on the substrate into the chemical liquid.5. The device of claim 1 , wherein the second supplier ...

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Номер записи: 33
05-03-2020 дата публикации

METHOD AND APPARATUS FOR THE ISOLATION AND TREATMENT OF PARTICULATE TARGETS

Номер: US20200071658A1
Автор: Niehren Stefan, Seeger Stefan
Принадлежит: Molecular Machines & Industries AG

A method of isolating/treating a particulate target using an apparatus comprising at least a capillary comprising a tip orifice and an internal cavity comprising a treatment liquid, a positioning element capable of positioning the tip orifice of the capillary, and a discharging/drawing element capable of causing a discharge of treatment liquid from the capillary and causing a drawing of treatment liquid into the capillary. The method comprises the steps of (1) locating the particulate target to be isolated and treated, (2) positioning the tip orifice such that the tip orifice comes in proximity to the particulate target, using the positioning element, (3) discharging a volume of treatment liquid to enclose the particulate target in the treatment liquid, (4) drawing the particulate target enclosed in the treatment liquid into the internal cavity of the capillary, and (5) treating the particulate target within the internal cavity of the capillary comprising the treatment liquid. 2. The method of isolating and treating a cell according to claim 1 , wherein the treatment liquid is not a growth medium.3. The method of isolating and treating a particulate target according to claim 1 , wherein the treatment liquid is a cell lysis buffer or a proteolytic treatment buffer.4. The method of isolating and treating a particulate target according to claim 1 , wherein the orifice of the capillary is positioned such that the orifice of the capillary comes at least within 20 μm claim 1 , preferably at least within 10 μm claim 1 , of the particulate target and/or wherein the particulate target has a size of from 100 nm to 100 μm claim 1 , preferably of from 500 nm to 50 μm and/or wherein the diameter of the tip orifice is in the range of 1 μm to 1000 μm claim 1 , preferably of from 10 μm to 100 μm.5. The method of isolating and treating a particulate target according to claim 1 , wherein the particulate target substrate is located on a substrate preferably chosen from microscope ...

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Номер записи: 34
02-04-2015 дата публикации

INTEGRATED MODULAR UNIT INCLUDING AN ANALYTE CONCENTRATOR MICROREACTOR DEVICE CONNECTED TO A CARTRIDGE-CASSETTE

Номер: US20150093304A1
Автор: GUZMAN Norberto
Принадлежит:

The integrated modular and interchangeable unit of the present invention comprises an analyte concentrator-microreactor (ACM) device having four entrance-exit ports identified as connection areas to a transport capillary for sample and buffer introduction, and to a separation capillary of a cartridge-cassette filled with an appropriate separation fluid for optimal separation of the analytes of interest. Affinity ligand groups are immobilized to microstructures contained within the main inner cavity or channel of the device or directly to the wall of the main inner channel for capturing one or more analytes. The inlet and outlet ends of the transport capillary are connected through a parallel design option to the analyte concentrator-microreactor (ACM) device to facilitate the path of sample and buffers through the inner cavity or channel of the analyte concentrator-microreactor (ACM) device. 1. An integrated modular unit comprising:a portable modular analyte concentrator-microreactor (ACM) device;a transport capillary or passage connected to the analyte concentrator-microreactor (ACM) device, the transport capillary or passage having an inlet end and an outlet end;a separation capillary or passage connected to the analyte concentrator-microreactor (ACM) device;the separation capillary or passage having an inlet end and an outlet end;the analyte concentrator-microreactor (ACM) device having a transport inlet port and a transport outlet port connected respectively to the inlet end and the outlet end of the transport capillary or passage, the transport inlet port and the transport outlet port of the analyte concentrator-microreactor (ACM) device being positioned in a parallel configuration to each other;the analyte concentrator-microreactor (ACM) device having a separation inlet port and separation outlet port connected respectively to the inlet end and the outlet end of the separation capillary or passage;the analyte concentrator-microreactor (ACM) device having an ...

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Номер записи: 35
21-03-2019 дата публикации

METHOD AND PRODUCT FOR PREPARING A PROTEIN-CONTAINING SAMPLE FOR ANALYSIS BY MASS SPECTROMETRY

Номер: US20190086306A1
Автор: Foster Greg A., Krawitzky Michael O., Lopez Ferrer Daniel, Robitaille Aaron M.
Принадлежит:

A method for preparing a protein-containing sample for analysis by mass spectrometry includes introducing the sample into a reaction vessel. The reaction vessel contains a reagent mixture including pre-measured quantities of an immobilized proteolytic enzyme, a reducing agent and an alkylating agent. The contents of the reaction vessel are activated by heating or by sonication. 1. A method for preparing a protein-containing sample for analysis by mass spectrometry , comprising:introducing the sample into a reaction vessel in which a reagent mixture is disposed, the reagent mixture including pre-measured quantities of an immobilized proteolytic enzyme, a reducing agent and an alkylating agent; andactivating the contents of the reaction vessel.2. The method of claim 1 , wherein the proteolytic enzyme is selected from the group consisting of trypsin claim 1 , LysC claim 1 , LysN claim 1 , AspN claim 1 , GluC claim 1 , ArgC and chymotrypsin.3. The method of claim 1 , wherein activating comprises sonicating the contents of the reaction vessel for between 30 seconds and 30 minutes.4. The method of claim 1 , wherein activating comprises heating the contents of the reaction vessel at a temperature between 37° C. and 60° C. for between 5 minutes and 90 minutes.5. The method of claim 1 , wherein activating comprises applying a pressure of between 500 psi and 45 kpsi for ten minutes or less.6. The method of claim 1 , wherein the sample contains between 10 ng and 1000 μg of protein.7. The method of claim 1 , wherein the sample contains between 1 cell and 1 billion cells.8. The method of claim 1 , wherein the reagent mixture includes a cell lysing agent.9. The method of claim 8 , wherein the cell lysing agent is selected from the group consisting of: a detergent in an amount between 0.01% and 5% (vol/vol) claim 8 , an organic solvent in a between 1% and 80% (vol/vol) claim 8 , and urea between 100 mM and 8M as calculated after sample is added.10. The method of claim 1 , wherein ...

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Номер записи: 36
26-06-2014 дата публикации

ROTATING SHIELDED MAGNETIC ACTUATOR

Номер: US20140179022A1
Автор: Schilffarth Adam, Smith Eric
Принадлежит: Luminex Corporation

Magnetic actuators comprising at least one shielded rotatable magnet are presented. Systems comprising such magnetic actuators and methods for using such magnetic actuators to isolate magnetic particles in a fluid are also presented. 1. A magnetic actuator configured to isolate particles in a fluid assay , the magnetic actuator comprising:a first shielded magnet comprising a first shield coupled to and covering a portion of a first permanent magnet, where the first shielded magnet has a thickness, a length, and a first axis of rotation;a motor coupled to the first shielded magnet and configured to rotate the first shielded magnet about the axis of rotation; anda lateral support member configured to support the first shielded magnet and the motor.2. The magnetic actuator of where the axis of rotation is an eccentric axis of rotation.3. The magnetic actuator of claim 1 , further comprising a second shielded magnet comprising a second shield coupled to and covering a portion of a second permanent magnet claim 1 , where the second shielded magnet has a thickness claim 1 , a length claim 1 , and a second axis of rotation.4. The magnetic actuator of claim 3 , where the second shielded magnet is coupled to the first shielded magnet such that rotation of the first shielded magnet about the first axis of rotation rotates the second shielded magnet about the second axis of rotation.5. The magnetic actuator of claim 1 , where the first permanent magnet is magnetized through its thickness.6. The magnetic actuator of claim 1 , where the first permanent magnet comprises a rare earth material.7. The magnetic actuator of claim 1 , where the first shield comprises a magnetic permeable material.8. A magnetic actuator configured to isolate particles in a fluid assay claim 1 , the magnetic actuator comprising:a first pair of shielded magnets rotatable together, each shielded magnet comprising a shield coupled to and covering at least a portion of a permanent magnet, where each shielded ...

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Номер записи: 37
26-03-2020 дата публикации

MASS SPECTROMETRY PROFILING OF ESOPHAGEAL CANCER MARKERS

Номер: US20200096519A1
Автор: Anderson Heather Rae, Lin Steven H., Seeley Erin Heather, Smoot Katy Ryan
Принадлежит:

This disclosure relates to the use of Matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) to assay tissue samples. Methods of analyzing a tissue sample may generally comprise generating sample ions directly from the tissue sample using a MALDI ionization source, receiving the ions into a mass spectrometer, identifying at least one esophageal tumor related compound in the sample from results from the mass spectrometer, comparing the at least one identified esophageal tumor related compound in the sample to one or more known esophageal tumor profiles, and identifying at least one condition related to the sample from the comparison of the at least one identified esophageal tumor related compound to the one or more known esophageal tumor profiles. The mass spectrometer may be a quadrupole mass spectrometer, a time of flight mass spectrometer, an Orbitrap mass spectrometer, or an ion trap mass spectrometer. 1. A method of analyzing a tissue sample comprising:(a) generating sample ions directly from the tissue sample using a MALDI ionization source;(b) receiving the ions into a mass spectrometer;(c) identifying at least one esophageal tumor related compound in the tissue sample from results from the mass spectrometer;(d) comparing the at least one identified esophageal tumor related compound in the tissue sample to one or more known esophageal tumor profiles; and(e) identifying at least one condition related to the tissue sample from the comparison of the at least one identified esophageal tumor related compound to the one or more known esophageal tumor profiles.2. The method of claim 1 , wherein the tissue sample comprises an esophageal tumor sample from a subject characterized as at least one of chemoradiation therapy responsive claim 1 , chemoradiation therapy partially responsive claim 1 , and chemoradiation therapy nonresponsive.3. The method of claim 1 , wherein the one or more known esophageal tumor profiles comprise at least one of ...

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Номер записи: 38
08-04-2021 дата публикации

DEVICE AND METHOD FOR CHEMICAL ANALYSIS

Номер: US20210102937A1
Автор: Abedi Mehdi, Fotouhi Bahram, Fotouhi Mohammed, Greenfield Edward Alvin, Javaherian Kashayar, Mollaaghababa Reza, Nawana Namal, Taslim Mohammad E.
Принадлежит:

A processing and detection system for detecting presence of at least one gluten protein in a food sample comprises a food processor including: a reservoir containing a process liquid for processing the food sample; a body that comprises a chamber configured to receive the food sample; and a pressing surface configured to press on the reservoir to cause the process liquid to exit the reservoir and mix with the food sample, thereby generating a processed food liquid; and an exit port configured to conduct the processed food liquid out of the food processor; and a cartridge including: at least one sensor configured to receive the processed food liquid and to generate an electrical signal in response to interaction with the at least one gluten protein in the processed food liquid, and an analyzer in electrical communication with the at least one sensor for detecting the electrical signal and determining the presence of the at least one gluten protein in the food sample based on the detected electrical signal. 131.-. (canceled)32. A cartridge , comprising:a first sensor comprising a first graphene layer disposed on an underlying substrate, wherein said first graphene layer is functionalized with a first antibody,a second sensor comprising a second graphene layer disposed on said underlying substrate, wherein said second graphene layer is functionalized with a second antibody,wherein said first antibody is different from said second antibody.33. The cartridge of claim 32 , further comprising a sample delivery component having an inlet for receiving a liquid sample and a plurality of channels for distributing a portion of said received liquid sample to each of said first and second sensor.34. The cartridge of claim 32 , wherein each of said first and second sensor comprises a plurality of conductive pads electrically coupled to the respective functionalized graphene layer to allow measuring at least one electrical property of that functionalized graphene layer.35. The ...

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Номер записи: 39
04-04-2019 дата публикации

FIELD MEASUREMENT OF SOIL ELEMENT CONCENTRATION

Номер: US20190101505A1
Автор: JURADO LUIS, Liu Miao
Принадлежит:

In an embodiment, a system for measuring soil element concentration in a field in real time is disclosed. The system comprises an extraction apparatus coupled to a mobility component configured to move the system in the agricultural field. The extraction apparatus configured to receive a plurality of soil samples successively from a soil probe coupled to the mobility component, when the mobility component is operating. The extraction apparatus containing an extractant solution that is a solvent of the soil samples. In addition, the extraction apparatus comprising a mixer that is configured to mix the soil samples with the extractant solution, thereby forming a solution mix. The system also comprises a chemical sensor coupled to the extraction apparatus, the chemical sensor configured to measure a concentration level of a soil element in the solution mix. Furthermore, the system comprises a processor coupled to the chemical sensor, the processor configured to calculate a concentration level of the soil element in each of the plurality of soil samples after the soil sample is received by the extraction apparatus and before a successive soil sample is received by the extraction apparatus. 1. A system for measuring soil element concentration in an agricultural field as the system moves through the agricultural field , comprising: the extraction apparatus configured to receive a plurality of soil samples successively from a soil probe coupled to the mobility component, when the mobility component is operating,', 'the extraction apparatus containing an extractant solution that is a solvent of the soil samples; and', 'the extraction apparatus comprising a mixer that is configured to mix the soil samples with the extractant solution, thereby forming a solution mix;, 'an extraction apparatus coupled to a mobility component configured to move the system in the agricultural field,'}a chemical sensor coupled to the extraction apparatus, the chemical sensor configured to measure ...

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Номер записи: 40
21-04-2016 дата публикации

COMPOSITIONS AND METHODS FOR REMOVING PETROLEUM OIL FROM SOIL

Номер: US20160109339A1
Автор: CASTILLO PALACIOS Mauricio, CUERO RENGIFO Raul, Russi Castillo Jennifer Melissa, Trujillo Montenegro Jhon Henry
Принадлежит: INTERNATIONAL PARK OF CREATIVITY

Described herein are compositions and methods for effectively removing from soil samples. By isolating the oil from the soil sample, it is possible to accurately evaluate the different properties of the oil in the sample. 1. A method for isolating petroleum oil from a soil sample , the method comprising:a. obtaining a sample comprising petroleum oil;b. contacting the sample with a digestion composition; andc. filtering the sample in order to isolate a filtrate, wherein the filtrate comprises the petroleum oil.2. The method of claim 1 , wherein the digestion composition comprises a polysaccharide.3. The method of claim 1 , wherein the digestion composition comprises chitosan.4. The method of or claim 1 , wherein the digestion composition comprises one or more organic solvents.5. The method of claim 4 , wherein the organic solvent comprises a linear or branched hydrocarbon claim 4 , a halogenated hydrocarbon claim 4 , or a mixture thereof.6. The method of claim 1 , wherein the digestion composition comprises chitosan claim 1 , hexane claim 1 , and dichloromethane.7. The method of claim 1 , wherein the soil sample comprises sandy soil claim 1 , sludge claim 1 , clay sediment claim 1 , silt claim 1 , sandy sediment claim 1 , or any combination thereof.8. A digestion composition comprising chitosan claim 1 , a linear or branched hydrocarbon claim 1 , and a halogenated hydrocarbon.9. A digestion composition comprising chitosan claim 1 , hexane claim 1 , and dichloromethane. This application claims priority upon U.S. Provisional Application Ser. No. 61/826,242, filed May 22, 2013. The application is hereby incorporated by reference in its entirety for all of its teachings.Oil exploration is a process which is not only costly and uncertain but also time-consuming, technology-intensive, and labor-intensive. Typically, a large capital investment is required. Many oil exploration techniques involve targeting traces of petroleum which are believed to be connected to, or ...

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Номер записи: 41
09-06-2022 дата публикации

METHOD FOR DETECTING SKATOLE IN A SAMPLE OF PIG ADIPOSE TISSUE

Номер: US20220178833A1
Автор: Hamel Matthieu, Scorsone Emmanuel
Принадлежит:

A method for detecting the presence of skatole in a sample of pig adipose tissue, wherein the method comprises at least the steps of: a) subjecting an organic extract of the adipose tissue sample to an electrochemiluminescence reaction; b) measuring the luminescence intensity during step a) and, if the measured luminescence intensity exceeds a threshold value, deducing the presence of skatole in the sample of adipose tissue. The method also makes it possible, if skatole is present in the organic extract, to determine the content thereof. 1. A method for detecting a presence of skatole in a sample of a pig adipose tissue , comprising at least the steps of:a) subjecting an organic extract of the sample of the adipose tissue to an electrochemiluminescence reaction;b) measuring an intensity of a luminescence produced during step a) and, if the measured luminescence intensity exceeds a predetermined threshold value, deducing the presence of skatole in the sample of the adipose tissue.2. The method of claim 1 , wherein the organic extract comprises an aprotic organic solvent claim 1 , less than 1 wt. % water claim 1 , and a ground salt.4. The method of claim 3 , wherein steps i) and ii) are carried out simultaneously.5. The method of claim 4 , wherein steps i) and ii) comprise heating the sample of the adipose tissue to a temperature of 100° C. to 150° C. in an open container claim 4 , or to a temperature lower than 100° C. with a vacuum drawing.6. The method of claim 2 , wherein the organic solvent is acetonitrile claim 2 , dimethylsulfoxide claim 2 , propylene carbonate or γ-butyrolactone.7. The method of claim 2 , wherein the heating of step iii) is carried out in a closed container claim 2 , at a temperature of 50° C. to 80° C. claim 2 , for 10 minutes to 30 minutes and with stirring.8. The method of claim 2 , wherein step iv) comprises centrifugating the organic solution obtained at the end of step iii) claim 2 , then fixing the fatty part of the organic solution by ...

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Номер записи: 42
24-07-2014 дата публикации

Hand-held adipose processor and cell concentrator

Номер: US20140207103A1
Автор: Anthony Yang, Josh Nelson, Rolf Wolters, Stuart K. Williams
Принадлежит: Tissue Genesis Inc

Devices and methods are provided for processing adipose tissue with a hand-held device. This device may include a processing chamber, a cannula, a vacuum source, a digestion area, and a product cell concentration chamber.

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Номер записи: 43
11-05-2017 дата публикации

AUTOMATED SAMPLING SYSTEM

Номер: US20170131319A1
Автор: Bock Randall G., Elkin Kyle R.
Принадлежит:

The automated sampling system has a water tight case that encloses a testing instrument. In the preferred embodiment, the testing instrument is a field-portable ion chromatography instrument designed to test water samples. In operation, a controller directs a syringe pump to draw fluid from outside the system through a specialized filter with a continuous flow lower reservoir. When directed by the controller, the syringe pump then injects the sample water into a testing portion of the field-portable ion chromatography instrument. 1. An auto-sampling system for a testing instrument , the system comprising:a watertight outer shell;a control unit enclosed within the shell;a syringe pump in communication with the control unit; (a) an unfiltered reservoir having an inlet and an outlet so that the unfiltered reservoir comprises a continuous flow reservoir;', '(b) a filtered reservoir smaller than the unfiltered reservoir; and,', '(c) a filter element positioned between the filtered reservoir and the unfiltered reservoir; a syringe pump having a plunger;, 'a sample inlet filter in hydraulic communication with the syringe pump, the inlet filter comprisinga check valve positioned between the syringe pump and the inlet filter;wherein the system is structured so that, when directed by the control unit, a plunger of the syringe pump is retracted and suction force from the syringe pump causes sample fluid to move from the unfiltered reservoir into the filtered reservoir and through the check valve, when further directed by the control unit, the plunger is advanced, so that the a sample fluid is injected back through the check valve and into a testing portion of an instrument.2. The system of wherein the system comprises an auto-sampling system for a field-portable ion chromatography instrument.3. The system of further comprising an inline digester in hydraulic communication with the syringe pump and the inlet filter.4. The system of wherein the inline digester boils sample fluid ...

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Номер записи: 44
01-09-2022 дата публикации

PREPARATION OF BIOLOGICAL CELLS ON MASS SPECTROMETRIC SAMPLE SUPPORTS FOR DESORBING IONIZATION

Номер: US20220276140A1
Автор: Sparbier Katrin, WEGEMANN Beatrix
Принадлежит:

The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution. 1. A method for the preparation of proteins from samples of unpurified biological cells on a sample support for the mass spectrometric determination of cellular properties , comprising the steps:providing the biological cells on a sample spot of the sample support,disrupting the cells on the sample spot using solvents and/or acids, whereby cell proteins separate from complexes, migrate out of the disrupted cells and are adhesively bonded to the sample spot,washing the cell proteins on the sample spot with buffer solution, andpreparing the washed cell proteins on the sample support for a subsequent desorbing ionization.2. The method according to claim 1 , wherein the washing step is conducted with a static buffer solution.3. The method according to claim 2 , wherein claim 2 , for the washing step claim 2 , several microliters of an aqueous solution are applied to the sample spot claim 2 , remain there for a predetermined period of time and are then removed.4. The method according to claim 1 , wherein the preparation for ...

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Номер записи: 45
01-09-2022 дата публикации

OPTIMIZED ANALYTE DERIVATIZATION FOR SYNERGISTIC APPLICATION WITH CRYSTAL SPONGE METHOD

Номер: US20220276186A1
Автор: Hierse Wolfgang
Принадлежит:

The invention provides a sample preparation method () comprising: providing a sample () comprising an organic molecule (), wherein the organic molecule () comprises a target group (), wherein the target group () is a nucleophilic group and/or an acidic group; a derivatization stage () comprising: derivatizing the target group () of the organic molecule () with a moiety () comprising one or more of (i) a hydrocarbon comprising group and (ii) a rd period atom comprising group, wherein the rd period atom is selected from the group consisting of Si, P, and S, thereby providing a derivatized organic molecule (); a separation stage () comprising: subjecting the sample () to a separation process to provide a fraction () comprising the derivatized organic molecule (); and a preparation stage () comprising: introducing the derivatized organic molecule () into a porous single crystal (), to provide a derivatized organic molecule doped porous single crystal (). 115-. (canceled)16. A sample preparation method comprising:providing a sample comprising an organic molecule, wherein the organic molecule comprises a target group, wherein the target group is a nucleophilic group, and/or an acidic group;{'sup': rd', 'rd, 'a derivatization stage comprising: derivatizing the target group of the organic molecule with a moiety comprising one or more of (i) a hydrocarbon comprising group and (ii) a 3period atom comprising group, wherein the 3period atom is selected from the group consisting of Si, P, and S, thereby providing a derivatized organic molecule;'}a separation stage comprising: subjecting the sample to a separation process to provide a fraction comprising the derivatized organic molecule; anda preparation stage comprising: introducing the derivatized organic molecule into a porous single crystal, to provide a derivatized organic molecule doped porous single crystal.17. The sample preparation method according to claim 16 , wherein the sample comprises a protic solvent claim 16 , ...

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Номер записи: 46
01-09-2022 дата публикации

METHODS AND APPARATUS FOR DETECTING ANALYTES

Номер: US20220276240A1
Автор: Cohen Raluca, DEUTSCH Omer, Krief Guy, Neumann Yoav
Принадлежит: SALIGNOSTICS LTD.

An elongate barrel is shaped to define a channel therethrough. A proximal region of the barrel defines an opening into the channel, and a distal region of the barrel defines an outlet from the channel. A sponge holding a body fluid sample is introduced into the opening by sliding a distal portion of a plunger, to which the sponge is coupled, through the channel. A porous carrier holding a reagent is disposed within the channel. Sliding the distal portion of the plunger distally through the channel drives the sample out of the sponge and through the carrier, dissolving at least some of the reagent. The sample is driven, with the dissolved reagent, through the outlet and onto a sample pad of a lateral flow platform. Other embodiments are also described. 1153-. (canceled)154. A method , comprising: [ an opening into the channel at a proximal region of the barrel,', 'an outlet from the channel at a distal region of the barrel,, 'an elongate barrel, the barrel shaped to define a channel therethrough, and having, 'a porous carrier, disposed within the channel;', 'a reagent, held in the carrier; and', 'a lateral flow platform, coupled to the distal region of the barrel such that a sample pad of the lateral flow platform is in fluid communication with the outlet:', 'introducing a sponge holding a sample of a body fluid, the sponge coupled to a distal portion of a plunger, into the opening of the channel; and', out of the sponge and through the carrier, dissolving at least some of the reagent, and', 'with the dissolved reagent, through the outlet, and onto the sample pad of the lateral flow platform., 'sliding the distal portion of the plunger distally through the channel, thereby compressing the sponge within the channel, driving the sample], 'using155. The method according to claim 154 , further comprising claim 154 , prior to the step of introducing:using the sponge, collecting an oral sample of saliva such that the sponge holds the sample.156. The method according to ...

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Номер записи: 47
02-05-2019 дата публикации

Reactive Diffusive Gradient in Thin-Film Sampler and Mercury Speciation by Use of Same

Номер: US20190128863A1
Автор: Looney Brian B.
Принадлежит:

Sampling devices for mercury speciation protocols are described. Devices can be utilized to separate mercury species from one another as a sample diffuses through a sampling device. Methods can determine the presence or quantity of targeted mercury species in a fluid sample. The devices are passive sampling devices based upon diffusion gradient in thin film (DGT) passive sampling devices. Devices include a reactant component and a sequesterant component that selectively react with a targeted species and retain a species (or a reaction product of a species) of a sample flow. Remaining mercury species can optionally be captured downgradient, for instance at an ion exchange resin. 1. A passive mercury sampling device comprising:an upgradient end and a downgradient end;a diffusive zone;a capture zone downgradient of the diffusive zone;a reactant located in either the diffusive zone or the capture zone, the reactant being configured to react with a first mercury species;a sequesterant located in either the diffusive zone or the capture zone, the sequesterant being configured to selectively retain a second mercury species.2. The passive mercury sampling device of claim 1 , wherein the reactant and the sequesterant are both located in the diffusive zone claim 1 , the device further comprising in the capture zone an agent configured to retain a third mercury species located in the capture zone.3. The passive mercury sampling device of claim 1 , wherein the reactant is upgradient of the sequesterant.4. The passive mercury sampling device of claim 3 , wherein the reactant is located in the diffusive zone and the sequesterant is located in the capture zone.5. The passive mercury sampling device of claim 1 , the diffusive zone and/or the capture zone each independently comprising one or more layers claim 1 , wherein the reactant and the sequesterant are located in the same layer.6. The passive mercury sampling device of claim 1 , the diffusive zone and/or the capture zone ...

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Номер записи: 48
25-05-2017 дата публикации

Pre-treatment Method for Determination of Volatile Thio-ether Compounds in Offensive Odorous Sediment

Номер: US20170146438A1
Автор: CHEN Kaining, GU Xiaozhi, KANG Jia, Liu Miao, SUN Shuyun, Wang Yadong
Принадлежит:

Method for determining volatile thio-ether compounds in offensive odorous sediment including: pre-treating a sediment sample, extracting volatile thio-ether compounds, separating and purifying the extraction supernatant in a solid phase extraction column, eluting with ethyl acetate, and finally collecting the eluent, which is then concentrated and used for determination by gas chromatography. 1. A pre-treatment method for the determination of volatile thio-ether compounds in offensive odorous sediment , comprising the following steps:(1) collecting a sample of an offensive odorous material and storing the sample away from light in a brown bottle to allow it to form a fresh sediment with a surface;{'sub': '2', '(2) adding 0.1% HgClto the surface of the fresh sediment for sterilization, while at the same time adding dry ice to keep the sample refrigerated and create an anaerobic environment on the surface of the fresh sediment; and finally placing the sample into a liquid nitrogen container for cold storage;'}(3) extracting a volatile thio ether compound from the sample in a two-step continuous extraction process, comprising a first step of alcohol/water exchange and a second step of ultrasonic extraction with a mixed extractant, whereinthe first step comprises repeating three times a procedure of first weighing some sediment sample in a centrifuge tube, adding absolute methanol and NaCl, or absolute ethanol and NaCl, so that a solid-to-liquid ratio of a resulting mixture with the sample is 1:2-1:5, shaking adequately, then centrifuging and collecting supernatant after standing still, and finally combing supernatant from the three repeated procedures for further use and keeping a residual sludge of the sample for use in the second step;the second step comprises adding a mixed extraction solution of methanol/cyclohexane or ethanol/cyclohexane at a ratio of 1:0.5-1:3 to the residual sludge from the first step at a solid-to-liquid ratio of 1:2-1:3, then performing three ...

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Номер записи: 49
07-05-2020 дата публикации

METHOD FOR EXTRACTING TARGET PROTEIN FROM BIOLOGICAL SAMPLE AND METHOD FOR ANALYZING TARGET PROTEIN

Номер: US20200140482A1
Автор: NITTA Shin-ichiro
Принадлежит:

An object of the present invention is to provide a method of efficiently extracting a target protein contained in a biological sample such as serum or plasma, thereby enabling highly precise analysis. In the present invention, there is provided the method in which a protein is removed from a sample derived from a living body to extract the target protein, and a salt and/or urea at a high concentration, and a water-soluble organic solvent are used. 1. A method of extracting a target protein from a sample derived from a living body by a deproteinization method , comprising:extracting the target protein by using a salt and/or urea at a high concentration, and a water-soluble organic solvent.2. The method according to claim 1 , wherein the water-soluble organic solvent is one or more selected from methanol claim 1 , ethanol claim 1 , isopropanol claim 1 , acetone claim 1 , and acetonitrile.3. The method according to claim 1 , wherein the salt is an ammonium salt claim 1 , a sodium salt claim 1 , a potassium salt claim 1 , a magnesium salt claim 1 , or a solution of each of the salts.4. The method according to claim 1 , wherein the salt is one or more salts selected from ammonium formate claim 1 , ammonium carbonate claim 1 , ammonium hydrogen carbonate claim 1 , ammonium acetate claim 1 , urea claim 1 , ammonium dihydrogen phosphate claim 1 , diammonium hydrogen phosphate claim 1 , sodium dihydrogen phosphate claim 1 , disodium hydrogen phosphate claim 1 , potassium dihydrogen phosphate claim 1 , dipotassium hydrogen phosphate claim 1 , sodium dihydrogen pyrophosphate claim 1 , sodium pyrophosphate claim 1 , potassium pyrophosphate claim 1 , sodium chloride claim 1 , potassium chloride claim 1 , magnesium chloride claim 1 , ammonium sulfate claim 1 , sodium sulfate claim 1 , sodium acetate claim 1 , sodium hydrogen carbonate claim 1 , and sodium carbonate.5. The method according to claim 1 , wherein the salt and/or urea at a concentration of 0.02 to 8 M is added into a ...

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Номер записи: 50
11-06-2015 дата публикации

SYSTEMS AND METHODS FOR PREPARING SAMPLES FOR CHEMICAL ANALYSIS

Номер: US20150160106A1
Автор: Emburgh Ron J., Kanipayor Ravi K.
Принадлежит:

A system for preparing samples for chemical analysis comprises at least one sample container, and a container receptacle apparatus for receiving the sample container. The sample container comprises an elongate tubular body having a crucible portion proximal to a closed end for receiving a sample therein, and an expansion portion proximal to an open end. The container receptacle apparatus comprising a housing having a heating compartment, a cooling compartment spaced apart from the heating compartment, and an insulating region located between the heating compartment and the cooling compartment. The heating compartment is shaped to receive the crucible portion of the sample container, and the cooling compartment is shaped to receive the expansion portion of the sample container. The apparatus also includes a heating mechanism for heating the sample within the crucible portion of the sample container, and a cooling mechanism for cooling the expansion portion of the sample container. 1. A system for preparing samples for chemical analysis , the system comprising:(a) at least one sample container for holding a sample to be analyzed, the sample container comprising an elongate tubular body extending from an open end to a closed end, the tubular body having a crucible portion proximal to the closed end for receiving the sample therein, and an expansion portion proximal to the open end; (i) a housing having a heating compartment, a cooling compartment spaced apart from the heating compartment, and an insulating region located between the heating compartment and the cooling compartment for thermally insulating the heating compartment from the cooling compartment, wherein the heating compartment is shaped to receive the crucible portion of the sample container and the cooling compartment is shaped to receive the expansion portion of the sample container;', '(ii) a heating mechanism for heating the sample within the crucible portion of the sample container while the sample ...

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Номер записи: 51
08-06-2017 дата публикации

LOW POWER SENSOR FOR NON-REACTIVE GASES

Номер: US20170160157A1
Автор: Chang Josephine B., Liu Jiaxing, van Kessel Theodore G.
Принадлежит:

Systems, devices, and methods are provided for detecting and measuring the concentration of non-reactive gases in a given environment, without having to increase the reactivity of the non-reactive gases through thermal heating. For example, a gas sensor device includes a sensing chamber, a chemical getter element disposed in the sensing chamber, and a pressure sensor device. The sensing chamber is configured to capture a gas sample. The chemical getter element is configured to remove reactive gas species of the gas sample through chemical reaction of the reactive gas species with the chemical getter element at room temperature. The pressure sensor device is configured to measure a pressure of non-reactive gas species of the gas sample, which remains in the sensing chamber after removal of the reactive gas species from the sensing chamber. The pressure measurement is used to determine an amount of the non-reactive gas species present in the sample. 1. A method , comprising:capturing a gas sample in a sensing chamber, the sensing chamber comprising a chemical getter element;removing reactive gas species of the gas sample through chemical reaction of the reactive gases with the chemical getter element at room temperature; andafter removing the reactive gas species from the sensing chamber, measuring a pressure of non-reactive gas species of the gas sample remaining in the sensing chamber.2. The method of claim 1 , wherein the gas sample comprises a sample of air in a given environment.3. The method of claim 1 , wherein the non-reactive gas species comprises methane.4. The method of claim 1 , wherein capturing the gas sample in the sensing chamber claim 1 , comprises:capturing the gas sample in a sampling chamber; andtransferring at least a portion of the gas sample from the sampling chamber to the sensing chamber.5. The method of claim 1 , wherein the chemical getter element comprises a material that reacts with nitrogen claim 1 , oxygen claim 1 , carbon dioxide claim ...

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Номер записи: 52
24-06-2021 дата публикации

METHOD FOR ENRICHING PATHOGEN, USING HOMOBIFUNCTIONAL IMIDOESTER

Номер: US20210189378A1
Автор: JIN Choong Eun, Shin Yong
Принадлежит: INFUSION TECH

The present invention relates to a method of enriching a pathogen using an homobifunctional imidoester group, and the method of enriching a pathogen and extracting nucleic acids using homobifunctional imidoester compound (DMA, DMP, DMS) according to the present invention can quickly extract a small amount of pathogen contained in a sample without the use of special equipment and can be used as in situ diagnosis, and since it is possible to enrich a pathogen and extract nucleic acids simultaneously in one tube or a chip, it has the advantage of being more efficient than the conventional method, saving time and cost, and being easy to use. 14-. (canceled)6. The method of enriching a pathogen of claim 5 , wherein the object of the first step is a thin film device claim 5 , a magnetic bead claim 5 , a ring resonator or a nanoparticle.7. The method of enriching a pathogen of claim 5 , wherein the object of the first step is modified with a silane compound.9. The method of enriching a pathogen of claim 8 , wherein the silane compound is at least one selected from the group consisting of (3-aminopropyl) triethoxysilane (APTES) claim 8 , (3-aminopropyl)trimethoxysilane) claim 8 , (1-aminomethyl)triethoxysilane claim 8 , (2-aminoethyl)triethoxysilane claim 8 , (4-aminobutyl)triethoxysilane) claim 8 , (5 -aminopentyl)triethoxysilane claim 8 , (6-aminohexyl)triethoxysilane claim 8 , 3-aminopropyl(diethoxy)methylsilane (APDMS) claim 8 , N-[3-(trimethoxysilyl)propyl]ethylenediamine claim 8 , N-[3-(trimethoxysilyl)propyl]diethylenetriamine claim 8 , [3-(2-aminoethylamino)propyl]trimethoxysilane (AEAPTMS) and 3-[(trimethoxysilyl)propyl]diethylenetriamine (TMPTA).10. The method of enriching a pathogen of claim 5 , wherein the sample containing a pathogen is any one selected from the group consisting of feces claim 5 , urine claim 5 , tears claim 5 , saliva claim 5 , external secretions from skin claim 5 , external secretions from respiratory tract claim 5 , external secretions from ...

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Номер записи: 53
22-06-2017 дата публикации

Substance sealing method and target molecule detecting method

Номер: US20170176430A1
Автор: Hiroyuki Noji, Lisa Yamauchi
Принадлежит: JAPAN SCIENCE AND TECHNOLOGY AGENCY

As a technique for efficiently sealing many substances, such as beads, nucleic acid, protein, virus, cells, and lipid membrane complex, into an array, the present invention provides a method for sealing a substance, including: (i) a step of introducing a first solvent containing a substance on a substrate on which a plurality of receptacles capable of storing the substance are formed separated from each other by a side wall; and (ii) a step of introducing a second solvent having a greater specific gravity than that of the first solvent onto the first solvent, the step (ii) being carried out after the step (i).

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Номер записи: 54
28-05-2020 дата публикации

DEVICE AND METHOD FOR PARTICLE COMPLEX HANDLING

Номер: US20200166440A1
Автор: LIU David J., Su Xing, SWARTZ Kenneth B., Wu Kai, YAMAKAWA Mineo
Принадлежит:

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte. 1. (Canceled)2. An analytical device comprising:at least one electromagnet functionally coupled a separate fluidic device to generate a magnetic field to move a target molecule comprising a ligand molecule coated thereon and magnetic particle without movement of the fluid in the fluid network;an integrated circuit to activate the at least one electromagnet; anda detector system comprising a light emitting diode and a camera for detecting the target molecule using reflectance.3. The device of claim 2 , wherein the fluid network comprises blood.4. The device of claim 2 , wherein the fluid network comprises saliva.5. The device of claim 2 , wherein the target molecule is present in a picomolar concentration.6. The device of claim 2 , wherein the target molecule is a protein.7. The device of claim 2 , wherein the magnetic particle is a nanoparticle.8. The device of claim 2 , wherein the magnetic field is configured to be selectively generated.9. The device of claim 2 , wherein the ligand comprises an antibody.10. The device of claim 2 , wherein the magnet is a substrate contacting the ...

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Номер записи: 55
28-05-2020 дата публикации

Method for Quantitative Analysis of Hydrogen in Porous Silica

Номер: US20200166452A1
Автор: Jieun Kim, Min Hwan Jung, Sumin Na, Sunah Shin
Принадлежит: LG Chem Ltd

A method for quantitative analysis of hydrogen gas generated due to the decomposition of Si—OH (silanol) in porous silica, which is a support of a metallocene catalyst is provided. The analysis enables the measurement of the content of hydrogen present in trace amounts in silica by employing an inert gas fusion-infrared absorption (IGFIA) method under specific pressure and temperature conditions.

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Номер записи: 56
08-07-2021 дата публикации

COMPOSITIONS, DEVICES, KITS AND METHODS USEFUL FOR REMOVAL OF PHOSPHOLIPIDS

Номер: US20210207189A1
Автор: Bouvier Edouard S. P., Chambers Erin E.
Принадлежит:

Novel compositions, devices, kits and methods useful for sample treatment are disclosed herein. 1. A method comprising: 'subjecting the digested sample to liquid chromatography to form an eluent comprising the at least one target analyte.', 'contacting a biological sample that comprises at least one phospholipid and at least one target analyte with a phospholipase enzyme in aqueous solution such that the phospholipid is enzymatically digested; and'}2. The method of claim 1 , wherein the sample fluid comprises a biological sample selected from a whole blood sample claim 1 , a plasma sample claim 1 , a serum sample claim 1 , a food sample claim 1 , and a food extract sample.3. The method of claim 1 , wherein the phospholipase enzyme is selected from Phospholipase A1 claim 1 , Phospholipase A2 claim 1 , Phospholipase B and Phospholipase C.4. The method of claim 1 , wherein the liquid chromatography is selected from reversed-phase chromatography (RPC) claim 1 , hydrophilic-interaction chromatography (HILIC) claim 1 , hydrophobic-interaction chromatography (HIC) claim 1 , ion-exchange chromatography (IEC) claim 1 , and normal-phase chromatography (NPC).5. The method of claim 1 , further comprising performing mass spectrometry analysis on at least a portion of the eluent.6. The method of claim 1 , wherein the biological sample is contacted with the phospholipase enzyme online with the liquid chromatography or wherein the biological sample is contacted with the phospholipase enzyme offline prior to the liquid chromatography.7. The method of claim 1 , wherein the phospholipase enzyme is a free enzyme.8. The method of claim 1 , wherein the phospholipase enzyme is attached to a solid support.9. An enzymatic support comprising a solid support and a phospholipase enzyme attached to the solid support.10. The enzymatic support of claim 9 , wherein the phospholipase enzyme is selected from Phospholipase A1 claim 9 , Phospholipase A2 claim 9 , Phospholipase B and Phospholipase C.11 ...

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Номер записи: 57
28-06-2018 дата публикации

RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

Номер: US20180180611A1
Автор: Deering Amanda, Foster Kirk Solon, Hardenstein Jaycey, Jones James Thomas, Kreke Thomas Richard, Ku Seockmo, Ladisch Michael Ralph, Liu Xingya (Linda), Tungare Alisha, Ximines Eduardo de Aquino
Принадлежит:

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability. 1. A method for detecting pathogens in food samples , the method comprising:obtaining a food sample;treating the food sample with an enzyme;microfiltering the treated food sample; andassaying the microfiltered food sample for presence of a pathogen,wherein cellular viability of the pathogen is maintained throughout the treating and microfiltering steps.2. The method of wherein the treated food sample comprises a solution.3. The method of further comprising preparing the food sample before the treating step wherein preparing comprises mechanically blending the food sample.4. The method of wherein the prepared food sample comprises coagulated proteins.5. The method of wherein the food sample comprises egg.6. The method of wherein the food sample comprises chicken.7. The method of claim 1 , wherein the food sample comprises spinach.8. The method of claim 1 , wherein the food sample comprises beef.9. The method of claim 1 , wherein the food sample comprises turkey.10. The method of claim 1 , wherein treating the food sample comprises hydrolyzing proteins in the food sample.11. The method of claim 10 , wherein the enzyme comprises is a protease.12. The method of wherein the treating step comprises incubating the food sample with the protease for less than about 90 minutes.13. The method of wherein the enzyme comprises a lipase.14. The method of wherein the assaying step comprises plating the microfiltered food sample on a selective media to detect the pathogen.15. The method of wherein the assaying step comprises a polymerase chain reaction (PCR)-based detection of nucleic acid of the pathogen. ...

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Номер записи: 58
04-06-2020 дата публикации

FIELD MEASUREMENT OF SOIL ELEMENT CONCENTRATION

Номер: US20200173954A1
Автор: JURADO LUIS, Liu Miao
Принадлежит:

In an embodiment, a system for measuring soil element concentration in a field in real time is disclosed. The system comprises an extraction apparatus coupled to a mobility component configured to move the system in the agricultural field. The extraction apparatus configured to receive a plurality of soil samples successively from a soil probe coupled to the mobility component, when the mobility component is operating. The extraction apparatus containing an extractant solution that is a solvent of the soil samples. In addition, the extraction apparatus comprising a mixer that is configured to mix the soil samples with the extractant solution, thereby forming a solution mix. The system also comprises a chemical sensor coupled to the extraction apparatus, the chemical sensor configured to measure a concentration level of a soil element in the solution mix. Furthermore, the system comprises a processor coupled to the chemical sensor, the processor configured to calculate a concentration level of the soil element in each of the plurality of soil samples after the soil sample is received by the extraction apparatus and before a successive soil sample is received by the extraction apparatus. 1. A system for measuring soil element concentration in an agricultural field as the system moves through the agricultural field , comprising:a mobility component configured to move through the agricultural field;a soil probe coupled to the mobility component and configured to obtain a succession of soil samples from different locations as the system moves through the agricultural field;an extraction apparatus coupled to the mobility component configured to add the succession of soil samples into a cartridge containing an extractant solution, thereby forming a solution mix;a chemical sensor coupled to the extraction apparatus configured to measure a concentration level of a soil element in the solution mix;a processor coupled to the chemical sensor, the processor configured to ...

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Номер записи: 59
05-07-2018 дата публикации

IMPROVED ASSAYS AND METHODS FOR ALLERGEN DETECTION

Номер: US20180188139A1
Автор: Brown Renuka Babu, Gilboa-Geffen Adi
Принадлежит:

The present invention relates to assays and methods for detecting the presence, absence and/or amount of an allergen (e.g., a food allergen) in a sample. The current assays and methods improve sampling processes, increase detection accuracy and sensitivity, and reduce the detection time. 1. A method for detecting an allergen in a test sample comprising:a). obtaining a test sample,b). receiving the test sample in a first chamber of a test container in a detection device through a reception port of the first chamber configured to receive the test sample,c). processing the test sample in the first chamber by homogenization,d). delivering the homogenized sample to a second chamber of the test container through a second port of the first chamber, wherein said second port is configured to deliver the homogenized sample in the first chamber to the second chamber, and wherein the second chamber is configured to receive the homogenized sample from the second port of the first chamber,e). processing the homogenized sample in an extraction buffer in the second chamber of the test container;f). contacting the processed sample with one or more detection agents, wherein said one or more detection agents specifically bind to said allergen, andg). observing a detection signal.2. The method of further comprising pre-processing the test sample in step (a) prior to receiving the test sample in the first chamber of the test container in the detection device claim 1 , wherein the test sample is pre-processed by cutting into small pieces claim 1 , crushing into powder claim 1 , smashing into small particles claim 1 , heating claim 1 , or a combination thereof.3. (canceled)4. The method of claim 1 , wherein the extraction buffer is optimized to achieve a maximal extraction of the allergen from the test sample.5. The method of claim 1 , wherein the step (e) further comprises providing the one or more detection agents to the analytic chamber within the test container.6. The method of claim ...

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Номер записи: 60
28-07-2016 дата публикации

METHOD OF EFFICIENT EXTRACTION OF PROTEIN FROM CELLS

Номер: US20160216258A1
Автор: Beadenkopf Robert J., Bruton Jeff H., Crews Virginia M., Ferguson Carrie S., Malick Adrien P., Mantlo John, Rosales Julie L.
Принадлежит:

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided. 121.-. (canceled)22. A method for detecting a target protein which is previously known or otherwise characterized , comprising: 1) contacting said cells with an extraction reagent to produce an intermediate composition having a pH of at least about pH 10.0; and', '2) contacting said intermediate composition with a neutralizing reagent to neutralize said pH of said intermediate composition and produce said protein extract,', 'wherein one or both of said extraction reagent and said neutralizing reagent comprises a polyoxyethylene alkyl ether; and, 'a) producing a protein extract from fixed or unfixed cells according to a method for producing a protein extract from fixed or unfixed cells, comprisingb) testing for the presence of said protein in said protein extract.23. The method of claim 22 , wherein said testing employs a capture agent for said target protein.24. The method of claim 22 , wherein said testing includes an immunological assay.25. The method of claim 24 , wherein said assay is an enzyme linked immunoabsorbant assay (ELISA).26. The method of claim 24 , wherein said assay is an immunochromatographic assay or lateral flow (LF) assay.27. The method of claim 26 , wherein said assay employs moncoclonal antibody-conjugated gold particles.28. The method of claim 24 , wherein said assay employs fluorescently- ...

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Номер записи: 61
02-08-2018 дата публикации

SILICON SUBSTRATE ANALYZING DEVICE

Номер: US20180217036A1
Автор: Ichinose Tatsuya, Kawabata Katsuhiko, Takuma Hayashi
Принадлежит: IAS INC.

A silicon substrate analyzing device with which impurities such as trace metals in a silicon substrate having a thick nitride film or oxide film formed on a silicon substrate surface can be analyzed with a high precision by ICP-MS. The silicon substrate analyzing device includes a load port, a substrate transportation robot, an aligner, a drying chamber, a gas-phase decomposition chamber, an analysis scan port having an analysis stage and a substrate analyzing nozzle, an analysis liquid collecting means, and an analysis means for performing inductively coupled plasma mass spectrometry. The silicon substrate having an oxide film or a nitride film formed on the silicon substrate is subjected to scanning the surface of the silicon substrate with a high-concentration recovered liquid with use of the substrate analyzing nozzle so that the high-concentration recovered liquid is recovered. The recovered high-concentration recovered liquid is discharged onto the surface of the silicon substrate and then heated and dried. The surface of the silicon substrate is scanned with the analysis liquid so that the impurities are recovered, and the analysis liquid is analyzed by ICP-MS. 1. A method of analyzing which comprises providing a silicon substrate analyzing device comprising:a load port on which a storage cassette that stores a silicon substrate to be analyzed is placed;a substrate transportation robot capable of taking out, transporting, and installing the silicon substrate stored in the load port;an aligner for adjusting a position of the silicon substrate;a drying chamber for heating and drying the silicon substrate;a gas-phase decomposition chamber for etching the silicon substrate with an etching gas;an analysis scan port having an analysis stage on which the silicon substrate is mounted, and a substrate analyzing nozzle for scanning a surface of the silicon substrate mounted on the analysis stage with an analysis liquid and collecting the analysis liquid into which the ...

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Номер записи: 62
02-08-2018 дата публикации

POTENTIAL GRADIENT AMPLIFIED DETECTION OF CHEMICAL AGENTS

Номер: US20180217085A1
Автор: Ali Mohammad Amdad, Braun Paul V.
Принадлежит: The Board of Trustees of the University of Illinois

New approaches for selective detection of chemical agents such as sarin are necessary because of the high toxicity of sarin and related compounds, the potential of these compounds to be used as weapons of mass destruction, and the limitations of current detection methodologies. Herein is described an apparatus and a method for selective and amplified detection of sarin simulants deposited via an aerosol process. The simulant absorbs into a hydrogel, where it hydrolyzes upon contact with water producing elemental ions. The elemental ions are then concentrated via an ionic chemical potential gradient to a sensor, where it is detected. This technique has potential to amplify the capture efficiency of a sensor by a 1000-fold within couple of minutes. 1. A polymer gel for detection of elemental ions comprising:a) a polymer gel having a chemical potential gradient that can concentrate elemental ions by enthalpy driven transport; andb) a plurality of functional groups, wherein the polymer gel is covalently bonded to the functional groups that provide the chemical potential gradient that transports the elemental ions to a region where the elemental ions are concentrated;wherein an analyte in contact with the polymer gel is dissociated into molecular ions and elemental ions, and the elemental ions are transported by the chemical potential gradient of functional groups to the region where the elemental ions are concentrated.2. The polymer gel of claim I wherein the functional groups comprise a quaternary amine moiety , a sulfate moiety , a nitrate moiety , a tertiary amine moiety , a borate moiety , or a combination thereof.3. The polymer gel of wherein the elemental ions are halide ions or hydrogen ions.4. The polymer gel of wherein the polymer gel comprises a catalyst dispersed in the polymer gel.5. The polymer gel of wherein the polymer gel comprises an enzyme dispersed in the polymer gel.6. The polymer gel of wherein the polymer gel comprises a polyacrylamide (PAAm) ...

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Номер записи: 63
12-08-2021 дата публикации

TETZ-PROTEINS AND PRION-LIKE PROTEINS AND ASSOCIATED METHODS

Номер: US20210247408A1
Автор: Tets Georgy Viktorovich, Tets Viktor Veniaminovich
Принадлежит:

The invention relates to diagnosis, prevention, and treatment of diseases and conditions associated with the functions of prion-like or Tetz-proteins. 1. A method of diagnosing a disease in a subject , which method comprises:a) heating a sample collected from the subject for 10 seconds to 48 hours at a temperature from 43° C. to 200° C.,b) isolating a protein fraction in the sample after the completion of the heating,c) determining the level of one or more polypeptides in the protein fraction isolated in step (b),d) comparing the level of the one or more polypeptides identified in step (c) with a control level(s) of said polypeptide(s), ande) (i) identifying the subject as being afflicted with the disease when the level(s) of said one or more polypeptides is different by 10% or more from the control level(s), or (ii) identifying that the subject is not afflicted with the disease if the level(s) of said one or more polypeptides differs from the control level(s) by less than 10%.2. A method of monitoring changes in development of a disease in a subject , which method comprises:a) heating a first sample collected from the subject for 10 seconds to 48 hours at a temperature from 43° C. to 200° C.,b) isolating a protein fraction in the first sample after the completion of the heating,c) determining the level of one or more polypeptides in the protein fraction isolated in step (b),d) repeating steps (a)-(c) for one or more additional samples, wherein said additional samples have been collected from the subject at later time points than the first sample,e) comparing the levels of the one or more polypeptides identified in step (c) with the level(s) of said polypeptide(s) identified in step (d), andf) (i) determining that the disease has progressed when the level(s) of the one or more polypeptides identified in step (d) is higher than the level(s) of said polypeptide(s) identified in step (c), or (ii) determining that the disease has not progressed when the level(s) of the ...

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Номер записи: 64
11-08-2016 дата публикации

METHOD AND DEVICE FOR INCREASING THE OPTICAL TRANSPARENCY OF REGIONS OF A TISSUE SAMPLE

Номер: US20160231209A1
Автор: SÄGMÜLLER Bernd
Принадлежит: Leica Microsystems CMS GmbH

A method for increasing the optical transparency of regions of a tissue sample () is proposed, in which the tissue sample () is introduced into a process chamber () and is infiltrated in the process chamber () with at least one process fluid (), and in which a removal of light-scattering structures in the tissue sample () is carried out. The method encompasses monitoring the optical transparency of the tissue sample (), at least during a clearing time period in which the tissue sample () is introduced into the process chamber () and in which the removal of the light-scattering structures in the tissue sample () is carried out, by means of an optical transparency measuring arrangement () associated with the process chamber (). An apparatus () for carrying out the method is also a subject of the invention. 1. A method for increasing the optical transparency of regions of a tissue sample , in which the tissue sample is introduced into a process chamber and is infiltrated in the process chamber with at least one process fluid , and in which a removal of light-scattering structures in the tissue sample is carried out ,wherein the method encompasses monitoring the optical transparency of the tissue sample, at least during a clearing time period in which the tissue sample is introduced into the process chamber and the removal of the light-scattering structures in the tissue sample is carried out, by means of an optical transparency measuring arrangement associated with the process chamber.2. The method according to claim 1 , in which an optical transparency measuring arrangement having a light source and a light-sensitive detector is used claim 1 , the tissue sample being arranged between the light source and the light-sensitive detector.3. The method according to claim 2 , in which at least one light property is influenced by at least one of the light furnished by the light source claim 2 , and at least one detection property of the light-sensitive detector.4. The method ...

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Номер записи: 65
10-08-2017 дата публикации

Compositions and Methods for Clearing a Biological Sample

Номер: US20170227430A1
Автор: Frank Marini, George Christ
Принадлежит: Wake Forest University Health Sciences

The disclosure provides improved materials and methods for optically clearing biological tissue that is subsequently used for deep tissue imaging analysis. Also provided is a description of a microscopic image acquisition methodology in which imagery of intact tissues are acquired to rapidly acquire microscopy data on a whole-organ scale to maximize cost effectiveness for biological microscopy and minimize time spent performing such analysis.

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Номер записи: 66
10-08-2017 дата публикации

CHEMICAL DIGESTION METHODS OF QUANTIFICATION FOR WATER AND DEBRIS MIXTURES

Номер: US20170227517A1
Автор: RHODES DAVID BRUCE, Simister Claire Emmalyn
Принадлежит:

With a water, particulate and fibre mixture, a method of quantifying fibre content may include providing a sample of the mixture, filtering the sample to produce a particulate and fibre mixture, burning the particulate and fibre mixture to produce a fibre sample, and dissolving the fibre sample to produce a fibre solution. The fibre solution may be analyzed to determine an elemental content of the fibre solution. The elemental content may be compared to a known elemental content to estimate the fibre content. 1. A method of quantifying fibre content of a water , particulate and fibre mixture , comprising:providing a sample of the water, particulate and fibre mixture;filtering the sample of the water, particulate and fibre mixture to produce a particulate and fibre mixture;burning the particulate and fibre mixture to produce a fibre sample;dissolving the fibre sample to produce a fibre solution;analyzing the fibre solution to determine an elemental content of the fibre solution; andcomparing the elemental content to a known elemental content to estimate the fibre content.2. The method of claim 1 , wherein the step of filtering comprises filtering the sample of the water claim 1 , particulate and fibre mixture with an ashless filter.3. The method of claim 1 , wherein the step of burning comprises thermally decomposing the particulate of the particulate and fibre mixture.4. The method of claim 3 , wherein the step of burning comprises ashing of the particulate and fibre mixture in an alumina crucible.5. The method of claim 1 , wherein the step of dissolving comprises digesting the fibre sample with hydrofluoric acid.6. The method of claim 1 , wherein the step of analyzing comprises using Inductively Coupled Plasma—Atomic Emission Spectrometry (ICP-AES) analysis.7. The method of claim 1 , wherein the elemental content comprises at least one elemental composition for B claim 1 , Ca claim 1 , Mg claim 1 , Na and Si.8. The method of claim 1 , wherein the elemental content ...

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Номер записи: 67
19-08-2021 дата публикации

MITOCHONDRIAL NUCLEIC ACID DEPLETION AND DETECTION

Номер: US20210254135A1
Автор: "OGrady Justin Joseph", Bartolome Nerea, Kay Gemma Louise, Martinez Antonio, Palacios Lourdes
Принадлежит:

The present invention provides a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host and having been subjected to treatment to lyse host cells present in the sample, and having been subjected to a treatment to deplete nucleic acid released from host cells within said sample or otherwise to render such nucleic acid unidentifiable, the method comprising: (a) depleting host mitochondria or host mitochondrial DNA (mtDNA) present in the treated sample; and (b) extracting and/or analysing remaining nucleic acid from the treated sample. Also provided is a related kit comprising reagents for performing the method. Further claimed is a method for isolating mitochondrial DNA after host cells have been lysed and host cell nucleic acids have been removed from the sample. 1. A method for depleting host nucleic acid in a biological sample , said sample having been previously obtained from an animal host and having been subjected to treatment to lyse host cells present within the sample , and wherein said sample is or has been subjected to a treatment to deplete nucleic acid released from host cells within said sample or otherwise to render such nucleic acid unidentifiable , the method comprising:(a) depleting host mitochondria or host mitochondrial deoxyribonucleic acid (mtDNA) present in the treated sample; and(b) extracting and/or analysing remaining nucleic acid from the treated sample.2. The method according to wherein step (a) comprises adding a detergent to said treated sample.3. The method according to claim 2 , wherein step (a) further comprises adding a nuclease to said treated sample.4. The method according to or claim 2 , wherein said detergent comprises: saponin claim 2 , digitonin claim 2 , Triton-X100 claim 2 , Nonidet claim 2 , NP-40 claim 2 , Tween-20 and/or filipin.5. The method according to claim 4 , wherein said saponin has a sapogenin content of at least 5%.6. The method according to ...

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Номер записи: 68
18-08-2016 дата публикации

System, method and devices for tissue-based diagnosis

Номер: US20160235392A1
Автор: Makoto Ogura, Samir Mitragotri, Sumit Paliwal
Принадлежит: UNIVERSITY OF CALIFORNIA

Disclosed are kits for at least partly liquefying tissue. The kit may include a liquefaction promoting medium (LPM). The LPM may include a non-ionic surfactant; a zwitterionic surfactant; and an abrasive material. The kit may include instructions. The instructions may direct a user to treat a tissue of a living subject by: applying the LPM together with the abrasive material to the tissue of the living subject; and transmitting energy to the tissue of the living subject through the abrasive material in the presence of the LPM effective to cause at least partial dissolution of one or more components of the tissue of the living subject.

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Номер записи: 69
19-08-2021 дата публикации

Degradable Microsphere and Use Thereof

Номер: US20210255074A1
Автор: Tian Meng, Wang HongWei, Wang Jianbin, Yin Junlong
Принадлежит:

The present invention provides a degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent, wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond that is cleavable through a chemical and/or light treatment, thereby resulting in the degradation of the degradable microbead. The present invention also provides a method of separating a target protein from a sample. By using the degradation of the degradable microbead to replace an elution step in protein purification, it is possible to select a combination of target protein and affinity ligand with a stronger affinity, thereby improving the protein purification efficiency. The method is especially suitable for the high-throughput preparation of multiple protein samples, for example providing a protein sample for electron microscope observation or mass spectrometry measurement. 1. A degradable microbead comprising a polymer molecule crosslinked by a crosslinking agent , wherein the polymer molecule and/or the crosslinking agent comprises a sensitive chemical bond , wherein the sensitive chemical bond is cleavable through a chemical and/or light treatment , thereby resulting in the degradation of the degradable microbead.2. The degradable microbead according to claim 1 , wherein the sensitive chemical bond is provided by a compound selected from the group consisting of cystamine-based compounds claim 1 , o-nitrophenylethyl alcohol-based compounds or o-nitrobenzyl alcohol-based compounds claim 1 , glycolide or lactide claim 1 , and polypeptides having an internal proteolytic enzyme cleavage site.3. The degradable microbead according to claim 1 , wherein the polymer molecule is selected from the group consisting of polyacrylic acid-based compounds claim 1 , polyacrylate-based compounds claim 1 , polyacrylamide-based compounds claim 1 , polyvinyl alcohol-based compounds claim 1 , and polyethylene glycol-based compounds; and the crosslinking agent is ...

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Номер записи: 70
19-08-2021 дата публикации

Sequential digestion of polypeptides for mass spectrometric analysis

Номер: US20210255075A1
Автор: Da Ren, Melissa Sato
Принадлежит: AMGEN INC

The disclosed methods are directed to preparing polypeptides for multi-attribute analysis. The polypeptides are optionally denatured, reduced, and/or alkylated before being subjected to a first digestion. Following the first digestion the large and small fragments resulting from the digestion are separated from each other. A second digestion is then performed on the larger of the fragments. All of the fragments from the two digestions are then analyzed chromatographically, electrophoretically, or spectrometrically, or a combination of these methods. The methods are especially useful for the preparation of therapeutic polypeptides for analysis, especially those that are not easily cleaved.

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Номер записи: 71
16-08-2018 дата публикации

METHOD FOR PARALLEL QUANTIFICATION OF PROTEIN VARIANT

Номер: US20180231563A1
Автор: HAMADA Akinobu, IWAMOTO Noriko, Shimada Takashi, Umino Yukari
Принадлежит:

There are numerous proteins having different in vivo effects depending on variants. In order to gain a more accurate understanding of an in vivo effect of a protein, when the protein in a sample is quantified, it is necessary to measure an amount of each variant that can be present. 1. A method for parallel determination of variants of a protein having 2 or more variants using mass spectrometry , comprising:protease-digesting a protein in a sample;detecting, using mass spectrometry, among peptides obtained by the protease digestion, 2 or more kinds of peptides having an amino acid sequence specific to each of the variants; anddetermining an amount of each of the variants in the sample based on a result of the mass spectrometry,wherein the variants are splicing variants of the protein associated with the production of new blood vessels or the growth of vascular endothelial cells.2. The method according to claim 1 , further comprising:detecting a peptide having an amino acid sequence common to 2 or more variants.3. (canceled)4. The method according to claim 1 , wherein the protein is a vascular endothelial growth factor (VEGF)-A.5. The method according to claim 4 , wherein the variants include 2 or more selected from the following splicing variants of VEGF-A: 206 (SEQ ID NO: 1) claim 4 , 189 (SEQ ID NO: 2) claim 4 , 183 (SEQ ID NO: 3) claim 4 , 165 (SEQ ID NO: 4) claim 4 , 148 (SEQ ID NO: 5) claim 4 , 145 (SEQ ID NO: 6) claim 4 , 121 (SEQ ID NO: 7) claim 4 , 165b (SEQ ID NO: 8) claim 4 , 121b (SEQ ID NO: 9) claim 4 , and 111 (SEQ ID NO: 10).6. The method according to claim 4 , wherein 1 or more peptides having amino acid sequences shown in SEQ ID NOs: 11-26 are detected.7. A kit for use in executing the method according to using high performance liquid chromatograph mass spectrometry claim 1 , comprising:a protease;a reaction container for digesting the protein by bringing the protein and the protease into contact with each other;a buffer solution for causing a ...

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Номер записи: 72
17-08-2017 дата публикации

Pre-analysis treatment device usable for amino acid, organic acid, and glucide and pre-analysis treatment method

Номер: US20170234843A1
Автор: Ryoichi Sasano
Принадлежит: AISTI Science Co Ltd

A pre-analysis treatment device usable for an amino acid, organic acid, and glucide includes an ion-exchange unit configured to load a test sample on a solid-phase cartridge S having a strong ion-exchange resin phase, to allow the strong ion-exchange resin phase to adsorb a predetermined organic compound, then supply a dehydration solvent to dehydrate the strong ion-exchange resin phase, and a derivatization unit configured to feed a predetermined amount of the derivatization reagent to the dehydrated strong ion-exchange resin phase to allow the derivatization reagent to retain for a predetermined time period, thereby trimethylsilylating the organic compound adsorbed on the strong ion-exchange resin phase, and simultaneously desorbing the trimethylsilylated organic compound from the strong ion-exchange resin phase, and then supply a push-out solvent to push the trimethylsilylated organic compound desorbed, out of the solid-phase cartridge S. The device enables at least one organic compound selected from amino acids, organic acids and glucides contained in a test sample to be derivatized and collected easily in a short period of time, and automation of the pre-analysis treatment.

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Номер записи: 73
25-07-2019 дата публикации

Method for agglutinating erythrocytes, method for separating erythrocytes, and hemagglutination reagent

Номер: US20190226952A1
Автор: Daisuke Kato, Shino MURAMATSU, Tomohiro HATTORI
Принадлежит: Denka Seiken Co Ltd

Methods of agglutinating and separating erythrocytes, by which erythrocytes can be instantaneously agglutinated into a sufficient size in a blood sample and completely separated from the blood sample; and a hemagglutination reagent are provided. The method of agglutinating erythrocytes according to the present invention includes adding a solution containing a cholic acid-based surfactant and an acid to a blood sample. The method of separating erythrocytes according to the present invention includes separating the erythrocytes agglutinated by the above-described method of the present invention. The hemagglutination reagent according to the present invention contains a cholic acid-based surfactant and an acid.

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Номер записи: 74
26-08-2021 дата публикации

Tandem-paired column chemistry for high-throughput proteomic exosome analysis

Номер: US20210263042A1
Автор: Kevin P. Rosenblatt, Malik KHALID, Najmuddin Khaja
Принадлежит: NX Prenatal Inc

Compositions and methods for sample preparation and mass spectrometric analysis of peptide samples obtained from biological samples are provided. The compositions and methods include a tandem column system in which a trap column is in fluid contact with an analytical column such as, for example, a HPLC column. As analytes are eluted from the analytical column, they can be passed to a detector (e.g., a mass spectrometer) for peptide analysis.

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Номер записи: 75
01-08-2019 дата публикации

METHOD AND SYSTEM FOR X-RAY FLUORESCENCE (XRF) ANALYSIS OF EXPLORATION SAMPLES

Номер: US20190234890A1
Автор: LINTERN Melvyn
Принадлежит:

A collector device for determining a metal in an exploration sample containing a concentration of the metal not directly detectable by X-ray fluorescence (XRF), comprises an adsorbent material capable of concentrating metal from a digestion mixture produced by digesting the exploration sample, which is configured for association with an analysis window of the XRF detector to facilitate determination of the amount of metal value in the exploration sample. A sample preparation vessel, method and system used to prepare exploration samples for analysis includes a vessel for receiving the exploration sample, a digestion tablet and a digestion medium; a closure to allow the vessel to be agitated to produce a digestion mixture comprising dissolved metal and the collector device. The closure and the collector device are coupled so that collector device is retrieved from the vessel by removing the closure. The digestion tablet includes a metal lixiviate and an alkali compound. 146-. (canceled)47. A method for preparing an exploration sample containing a metal or metalloid not directly detectable by X-ray fluorescence (XRF) for analysis by an XRF detector comprising:mixing the exploration sample, a digestion medium and a digestion composition or a digestion tablet containing an alkali compound and a metal lixiviant in an amount capable of dissolving the metal in the exploration sample in the digestion medium to produce a digestion mixture containing dissolved metal; and,contacting the digestion mixture with a collector device comprising an adsorbent material capable of concentrating dissolved metal from the digestion mixture, wherein the adsorbent material is configured for association with an analysis window of the XRF detector to facilitate determination by the XRF detector of the amount of metal in the exploration sample.48. A system for preparing an exploration sample containing a metal or a metalloid not directly detectable by XRF for analysis by an XRF detector ...

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Номер записи: 76
23-07-2020 дата публикации

EXTRACTION REAGENT OF IMMUNOSUPPRESSANT DRUG FOR IMMUNOASSAYS

Номер: US20200232975A1
Автор: Nie Yongyan, Shi Xiaojuan, Wu Fengbo
Принадлежит:

The disclosure provides a reagent for extracting immunosuppressant drugs from whole blood sample for immunoassay. The extraction reagent comprises protein denaturant, proteolytic enzyme, surfactant and pH buffer. The disclosure also provides a method and an immunoassay kit for detection of the immunosuppressant concentration in a whole blood sample using the extraction reagent. The extraction reagent of the present disclosure doesn't need the use of organic solvent as that in the traditional extraction method, therefore the adverse effects of the organic solvent on the antibody activity in a detection system and the other relative defects associated to its use are obviated. The drug extraction process of the present disclosure doesn't need centrifugation, the processed sample can be directly applied for immunoassay. The operation for drug extraction by the present disclosure is simple, and the detection result based on this extraction method is accurate. 1. A method for detecting immunosuppressant drug in whole blood sample , wherein the blood sample is processed via heating with an extraction reagent of the immunosuppressant drug in blood sample for immunoassay , and then determining the concentration of a drug contained therein by immunoassay , and the extraction reagent of the immunosuppressant drug in blood sample for immunoassay , comprising a protein denaturant , proteolytic enzyme , surfactant and pH buffer.2. The method according to claim 1 , wherein the protein denaturant is selected from urea claim 1 , guanidine hydrochloride or other non-organic solvent based denaturants.3. The method according to claim 2 , wherein the molar concentration of the urea in the extraction reagent is 4 mol/L to 12 mol/L; and alternatively the molar concentration of the guanidine hydrochloride in the extraction reagent is about 1 mol/L to 8 mol/L.4. The method according to claim 3 , wherein the molar concentration of the urea in the extraction reagent is 6 mol/L to 8 mol/L; and ...

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Номер записи: 77
09-09-2021 дата публикации

TECHNIQUES FOR TOXIC METAL DETECTION AND SPECIATION IN AQUEOUS MATRICES

Номер: US20210278389A1
Автор: Dozortsev Vladimir, Saini Harmesh K.
Принадлежит:

An in-situ measurement apparatus automatically draws aqueous samples on an intermittent or ad-hoc basis and measures specific metal specie concentration. The apparatus can perform both raw measurement of specific metal specie, as well as processing to convert other species of the same metal to the specific metal specie or to destroy or remove unwanted masking agents (e.g. organics). In one application, “dirty” water from a scrubber is measured for Se(IV) presence (using a renewable voltametric system), both with and without the masking agents present; in addition, selective processing converts other selenium species to Se(IV), permitting assessment of total selenium and measurement of Se(VI) presence. Automated reactions can then be taken to remove detected toxic substances from waste water without excess reliance on treatment chemicals, and so as to ensure that only water complaint with regulatory standards is released into the environment. 1. (canceled)2. A method of processing waste water , the method comprising:using a voltametric measurement device to measure concentration of Selenium (IV) in a first sample of the waste water;processing a second sample of the waste water to convert a second Selenium form to Selenium (IV), wherein the second Selenium form comprises a water soluble form of at least one of organo-Selenium, Selenium (0), Selenium (II) or Selenium (VI) to Selenium (IV);using the voltametric measurement device to measure concentration of Selenium (IV) in the second sample;with at least one processor, using measured concentration of Selenium (IV) in the first sample and using measured concentration of Selenium (IV) in the second sample to determine concentration of the second Selenium form in the waste water; andcontrolling application of at least one water treatment process, dependent on the determined concentration of the second Selenium form, to lessen concentration of the second Selenium form in the waste water.3. The method of claim 2 , wherein ...

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Номер записи: 78
20-11-2014 дата публикации

DEVICE AND METHOD FOR PARTICLE COMPLEX HANDLING

Номер: US20140342470A1
Автор: LIU David J., Su Xing, SWARTZ Kenneth B., Wu Kai, YAMAKAWA Mineo
Принадлежит:

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte. 1. A method comprisingproviding a device comprising a fluidic network comprising a magnetic particle in a fluid,generating a magnetic field coupled to the fluidic network, andtransporting the magnetic particle by the magnetic field in the fluidic network without fluidic movement of the fluid in the fluidic network.2. The method of claim 1 , wherein the magnetic particle comprises a magnetic affinity complex bound to an analyte.3. The method of claim 1 , wherein the magnetic particle comprises a magnetic affinity complex and a signal affinity complex claim 1 , both bound to different bind sites of an analyte.4. The method of claim 3 , wherein the analyte is a protein claim 3 , an antibody claim 3 , or a nucleic acid.5. The method of claim 1 , wherein the magnetic particle comprises a magnetic affinity complex or a signal affinity complex claim 1 , bound to an analyte claim 1 , wherein the magnetic affinity complex and the signal affinity complex are configured to bind to a same binding site of the analyte.6. The method of claim 5 , wherein the analyte is a small molecule.7. The method ...

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Номер записи: 79
30-09-2021 дата публикации

Non-Specific Nucleases from the Genus Pseudomonas for Use in Facilitating Flow of Cells Through a Microfluidic Channel

Номер: US20210301273A1
Автор: Elleuche Skander, Miltenyi Stefan, Nolle Volker, Schmitz Sarah
Принадлежит:

The present invention provides a composition comprising an isolated polypeptide having non-specific nuclease (NSN) activity for degrading nucleic acids comprising or consisting of at least 70% amino acid sequence identity to SEQ ID NO:2, wherein said polypeptide has NSN activity in a solution of about 4° C., and a cation complexing agent such as EDTA. Said composition may have preferentially a neutral to basic pH. A kit comprising said composition and a method using said composition are also disclosed. 115-. (canceled)16. A combination comprising:(i) an isolated recombinant nuclease containing an amino acid sequence that is at least 90% identical to SEQ ID NO:2, wherein the nuclease has non-specific nuclease (NSN) activity in solution at 4° C., and(ii) a non-naturally occurring cation complexing agent.17. The combination of claim 16 , wherein the nuclease is non-naturally occurring claim 16 , and the amino acid sequence of the nuclease comprises at least one amino acid substitution claim 16 , addition claim 16 , or deletion compared with SEQ ID NO:2.18. The combination of claim 17 , wherein the nuclease has increased NSN activity as a consequence of having an amino acid sequence that comprises at least one amino acid substitution compared with SEQ. ID NO:2.19. The combination of claim 18 , wherein the amino acid sequence of the nuclease comprises SEQ ID NO:3 or SEQ ID NO:6.20. The combination of claim 16 , wherein the NSN activity of the nuclease has a kvalue of at least 10 sat 4° C.21. The combination of claim 16 , wherein the cation complexing agent is EDTA (ethylenediaminetetraacetic acid).22. The combination of claim 16 , wherein the cation complexing agent is selected from EGTA (ethylene glycol-bis(2-aminoethylether)-N claim 16 ,N claim 16 ,N′ claim 16 ,N′-tetraacetic acid) claim 16 , DOTA (1 claim 16 ,4 claim 16 ,7 claim 16 ,10 tetraazacyclododecane-1 claim 16 ,4 claim 16 ,7 claim 16 ,10 tetraacetic acid) claim 16 , DTPA (diethylenetriaminepentaacetic acid) ...

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Номер записи: 80
14-09-2017 дата публикации

Temperature influenced chemical vaporization and detection of compounds having low volatility

Номер: US20170261483A1
Автор: Andrey N. Vilkov, Jack A. Syage, Joseph A. Widjaja
Принадлежит: Rapiscan Systems Inc

The present disclosure is directed to methods and systems for detecting a chemical substance. The methods and systems include chemically modifying a sample of a substance of interest through combination with a reagent to increase the volatility of the substance of interest. The systems and methods further include performing an analysis of the substance of interest.

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Номер записи: 81
06-08-2020 дата публикации

Native Microfluidic CE-MS Analysis of Antibody Charge Heterogeneity

Номер: US20200249241A1
Автор: LI NING, Qiu Haibo, Wang Hongxia
Принадлежит:

Methods for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample. The methods including: contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments; separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis; eluting separated antibody fragments from the one or more capillaries; and determining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest. 1. A method for detecting and/or discriminating between post-translational modification variants of an antibody of interest in a sample , comprising:contacting a sample comprising one or more antibodies of interest with a protease to digest the sample into antibody fragments;separating antibody fragments by molecular weight and/or charge in one or more capillaries using capillary electrophoresis;eluting separated antibody fragments from the one or more capillaries; anddetermining the mass of the eluted antibody fragments by mass spec analysis, thereby detecting and/or discriminating between post-translational modification variants of the antibody of interest.2. The method of claim 1 , wherein the post-translational modification comprises one or more of deamidation claim 1 , oxidation claim 1 , glycation claim 1 , disulfide formation claim 1 , N-terminal pyroglutamate formation claim 1 , C-terminal lysine removal claim 1 , and high mannose glycosylation.3. The method of claim 1 , wherein the protease comprises IdeS.4. The method of claims 1 , wherein the antibody fragments comprise one or more of an F(ab′)or Fc antibody subunit.5. The method of claim 1 , wherein the antibody of interest is a monoclonal antibody.6. The method of claim 1 , wherein the antibody fragments are separated by charge and the ...

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Номер записи: 82
11-12-2014 дата публикации

INDEPENDANT HEATING OF SAMPLES IN A SAMPLE HOLDER

Номер: US20140360287A1
Автор: DEBAN Ramin, FEILDERS George, GAUTHIER Jules, GUAY Raymond, ROSS Arthur
Принадлежит:

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample. 1. A method for heating a sample material in a sample holder , the method comprising:receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein;dynamically forming an individual mini microwave cavity around the sample recipient; andapplying microwaves generated by at least one microwave generator directly to the sample.2. The method of claim 1 , wherein dynamically forming the mini microwave cavities comprises mating a set of first partial mini cavities with a set of complementary second partial mini cavities.3. The method of claim 2 , wherein the set of first partial mini cavities are in the heating chamber and the set of complementary second partial mini cavities are on the sample holder.4. The method of claim 1 , further comprising mechanically positioning the sample holder in the heating chamber to align the set of first partial mini cavities with the set of complementary second partial mini cavities.5. The method of claim 1 , wherein dynamically forming an individual mini microwave cavity comprises forming a plurality of individual mini microwave cavities around each one of a plurality of sample recipients in the sample holder.6. The method of claim 5 , wherein applying microwaves comprises applying microwaves generated using at least two separate microwave generators.7. The method of claim 6 , wherein applying microwaves comprises applying microwaves according to an independent heating schedule with sample specific parameters for ...

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Номер записи: 83
29-08-2019 дата публикации

GAS SENSOR

Номер: US20190265180A1
Автор: Aoyama Shigeya, Inoue Tsuyoshi, NISHIO Kenji, SHICHIDA Takafumi, UEKI Masatoshi
Принадлежит: NGK SPARK PLUG CO., LTD

Disclosed is a gas sensor including: a wiring board; a sensor element mounted on the wiring board and electrically connected to the wiring board by conductive members; a casing installing the sensor element and formed with inlet and outlet ports; and a pretreatment unit configured to perform pretreatment on a measurement gas and feed the measurement gas to the inlet port. The inlet and outlet ports are located outward and upward of the sensor element. A protrusion is provided protruding toward the inside of the casing so as to narrow a flow path from the inlet port to the outlet port. A height from the sensor element to a distal end of the protrusion is lower than heights from the sensor element to the inlet and outlet ports. The protrusion is disposed more inside than respective top portions of the conductive members without being in contact with the conductive members. 1. A gas sensor , comprising:a wiring board extending in a longitudinal direction;a sensor element configured to detect a specific gas component in a measurement gas, the sensor element being disposed inside an outer circumference of one surface of the wiring board and being electrically connected to the wiring board by a plurality of conductive members;a casing defining an installation space in which the sensor element is installed, the casing having formed thereon an inlet port through which the measurement gas is introduced into the installation space and an outlet port through which the measurement gas is discharged out from the installation space; anda pretreatment unit configured to pretreat the measurement gas so as to adjust the concentration of the specific gas component in the measurement gas, and then, feed the pretreated measurement gas to the inlet port,wherein, assuming that a direction in which the sensor element faces the wiring board is a downward direction, the inlet and outlet ports are located at positions outside an outer circumference of the sensor element and upward of the ...

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Номер записи: 84
05-10-2017 дата публикации

CELL TRAPPING METHOD, METHOD FOR PRODUCING SPECIFIC CELL- TRAPPING DEVICE, AND METHOD FOR PRODUCING SPECIFIC CELL-CONTAINING SOLUTION

Номер: US20170282180A1
Автор: Kikuhara Yoshihito, TAKAI Kenji, Tsai Anthony H., Yagi Satomi
Принадлежит:

Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter. 1. A cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device , the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior , and a discharge flow path for discharging the liquid to the exterior; and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction , the method comprising:a step of draining a liquid to the cell trapping device for n (n is a natural number) times,wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the ...

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Номер записи: 85
05-09-2019 дата публикации

Chemical Vaporization and Detection of Compounds Having Low Volatility

Номер: US20190271679A1
Автор: Hanold Karl A., Syage Jack A., Vilkov Andrey N., Widjaja Joseph A.
Принадлежит:

The present disclosure is directed to methods and systems for detecting a chemical substance. The methods and systems include chemically modifying a sample of a substance of interest through combination with a reagent to increase the volatility of the substance of interest. The systems and methods further include performing an analysis of the substance of interest. 1. A method for detecting a substance of interest , the method comprising:collecting a sample of a substantially non-volatile substance of interest on a sampling trap;depositing a reagent on the trap, wherein the reagent increases the volatility of the substance of interest;introducing the trap including the substance of interest into a thermal desorber;vaporizing the substance of interest;transferring the vaporized substance of interest from the desorber into a detector;performing an analysis of the substance of interest; and,detecting the substance of interest.2. The method of claim 1 , wherein the reagent includes at least one of an organic acid claim 1 , an inorganic acid and a reducing agent.3. The method of claim 2 , wherein the reagent includes at least one of sulfuric acid claim 2 , sulfonic acid claim 2 , phosphonic acid claim 2 , phosphoric acid and combinations thereof.4. The method of claim 1 , wherein the substance of interest includes at least one of sodium nitrate claim 1 , potassium nitrate claim 1 , strontium nitrate claim 1 , barium nitrate claim 1 , sodium chlorate claim 1 , potassium chlorate claim 1 , sodium perchlorate claim 1 , potassium perchlorate claim 1 , sodium permanganate claim 1 , potassium permanganate claim 1 , and combinations thereof.5. A method for detecting a substance of interest claim 1 , the method comprising:collecting a sample of a substantially non-volatile substance of interest on a sampling trap;introducing the trap including the substance of interest into a thermal desorber;depositing a reagent on the trap within the desorber, wherein the reagent increases the ...

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Номер записи: 86
16-12-2021 дата публикации

DESALTING DEVICES AND PRESSURE-RESISTANT SIZING MEDIA

Номер: US20210387177A1
Автор: Boardman Anna, Lauber Matthew A., LI Wenjing, Muriithi Beatrice, Xu Mingcheng
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The present disclosure relates to a system for separating a sample including a device and a positive pressure source. The device can include a housing with a proximal end having an interface and a proximal end opening, a distal end opening opposite the proximal end opening, and an interior wall defining an interior of the housing; a bottom frit connected to the interior wall, extending across the interior of the housing, and located proximately to the distal end to minimize sample loss; and a resin disposed within the interior of the housing between the bottom frit and the proximal end. The positive pressure source can connect to the interface of the proximal end to apply positive pressure to the sample. A controller can control the applied positive pressure to the sample via the positive pressure source according to a relationship between the bottom frit, the resin, and the positive pressure. 1. A system for separating a sample comprising: a housing with a proximal end having an interface and a proximal end opening, a distal end opening opposite the proximal end opening, and an interior wall defining an interior of the housing;', 'a bottom frit connected to the interior wall, extending across the interior of the housing, and located proximately to the distal end to minimize sample loss; and', 'a resin disposed within the interior of the housing between the bottom frit and the proximal end;, 'a device comprisinga positive pressure source connected to the interface of the proximal end to apply positive pressure to the sample; anda controller configured to control the applied positive pressure to the sample via the positive pressure source according to a relationship between the bottom frit, the resin, and the positive pressure.2. The system of claim 1 , wherein the resin is a pressure-resistant resin to desalt the sample.3. The system of claim 1 , wherein the positive pressure source is a handheld pipette or a positive pressure manifold.4. The system of claim 1 , ...

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Номер записи: 87
25-12-2014 дата публикации

Systems and methods for preparing samples for chemical analysis using a cooled digestion zone

Номер: US20140377880A1
Автор: Ravi K. Kanipayor, Ron J. Emburgh
Принадлежит: 7685297 Canada Inc

An apparatus for preparing samples for chemical analysis includes a container receptacle for receiving a sample container having a crucible portion and an expansion portion. The container receptacle includes a heating compartment and a cooling compartment spaced apart from the heating compartment. The heating compartment is shaped to receive the crucible portion of the sample container, and the cooling compartment is shaped to receive the expansion portion of the sample container. The apparatus also includes a heating mechanism for heating the sample within the crucible portion of the sample container, a first cooling mechanism for cooling the expansion portion of the sample container, and a second cooling mechanism for cooling the crucible portion of the sample container.

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Номер записи: 88
23-12-2021 дата публикации

METHODS FOR HEAT-ASSISTED ENZYME DIGESTION

Номер: US20210396632A1
Автор: Boardman Anna, Lauber Matthew A., LI Wenjing, Muriithi Beatrice
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The present disclosure relates to a kit for sample preparation, the kit including a solid support surface with a polymer coating covering the solid support surface, wherein the polymer coating reduces undesired interactions between the sample and the solid support surface, a buffer comprising arginine and methionine, and a vessel for containing the solid support surface and the buffer. 1. A kit for sample preparation , the kit comprising:a solid support surface with a polymer coating covering the solid support surface, wherein the polymer coating reduces undesired interactions between a sample and the solid support surface;a container containing a buffer comprising arginine with a maximum concentration of about 20 mM and methionine ranging in concentration from about 100 mM to about 300 mM; anda vessel for containing the solid support surface and the buffer.2. The kit of claim 1 , wherein the buffer is in-solution.3. The kit of claim 1 , wherein the buffer further comprises Tris-HCl ranging in concentration from about 50 mM to about 200 mM.4. The kit of claim 3 , wherein the Tris-HCl concentration is about 100 mM.5. The kit of claim 1 , wherein the buffer further comprises CaClranging in concentration from about 1 mM to about 50 mM.6. The kit of claim 5 , wherein the CaClconcentration is about 100 mM.7. The kit of claim 1 , wherein the buffer further comprises a polyol claim 1 , wherein the polyol is selected from the group consisting of xylitol claim 1 , erythritol claim 1 , glycerol claim 1 , propylene glycol claim 1 , and butanediol.8. The kit of claim 7 , wherein the polyol is xylitol and the concentration of xylitol ranges from about 600 mM to about 680 mM.9. The kit of claim 1 , wherein the arginine concentration is about 10 mM.10. The kit of claim 1 , wherein the methionine concentration is about 200 mM.11. The kit of claim 1 , wherein the polymer coating comprises two portions claim 1 , a first coating portion with functionality for bioconjugation and a ...

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Номер записи: 89
22-10-2015 дата публикации

Substrates and Methods for Preparing Samples for Mass Spectrometry

Номер: US20150299761A1
Автор: Hattan Stephen J.
Принадлежит: VIRGIN INSTRUMENTS CORPORATION

A mass spectrometry substrate for performing adsorption and biological processing includes a porous silica-based material comprising a chemical adsorption material that captures analyte(s) of interest. A biologically active material incorporated into the porous silica-based material performs biological processing on the analyte(s) of interest. 1. A mass spectrometry substrate for performing adsorption and biological processing , the mass spectrometry substrate comprising:a) a porous silica-based material comprising a chemical adsorption material that captures analyte(s) of interest; andb) a biologically active material incorporated into the porous silica-based material, the biologically active material performing biological processing on the analyte(s) of interest.2. The mass spectrometry substrate of wherein the biologically active material comprises an enzyme and the biological processing comprises enzymatic digestion.3. The mass spectrometry substrate of wherein the enzymatic digestion comprises enzymatic digestion of protein(s) and polypeptides into their constituent peptide fragments.4. The mass spectrometry substrate of wherein the enzymatic digestion is accomplished by immobilization of a naturally occurring enzyme.5. The mass spectrometry substrate of wherein the enzymatic digestion is accomplished by immobilization of a synthetic or artificial enzyme.6. The mass spectrometry substrate of wherein the analyte(s) of interest are captured by hydrophobic interaction.7. The mass spectrometry substrate of wherein the analyte(s) of interest are captured by electrostatic interaction.8. The mass spectrometry substrate of wherein the chemical adsorption material causes a chemical interaction that is based on differential charge between the analyte(s) and the mass spectrometry substrate.9. The mass spectrometry substrate of wherein the chemical adsorption material causes a chemical interaction that is based on hydrophobic interactions between the analyte(s) and the ...

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Номер записи: 90
03-11-2016 дата публикации

Method for determining a concentration of metal impurities contaminating a silicon product

Номер: US20160320275A1
Автор: Carl W. Puehl, Dale Franklin Workman, Douglas H. Kreszowski
Принадлежит: Hemlock Semiconductor Operations LLC

A method determines a concentration of metal impurities contaminating a silicon product. The method comprises obtaining a test sample of the silicon product with the metal impurities disposed thereon. The test sample is placed within a first vessel. A first acid solution is added to the first vessel containing the test sample. The test sample is submerged into the first acid solution to produce a mixed solution comprising the first acid solution, the metal impurities, and digested silicon. The undigested silicon is sep crated from the mixed solution. The mixed solution is analyzed to determine the concentration of metal impurities contaminating the silicon product.

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Номер записи: 91
03-10-2019 дата публикации

Sample Preparation for Antimicrobial Susceptibility Testing

Номер: US20190301987A1
Автор: Flentie Kelly, Spears Benjamin, Stern Eric, Vacic Aleksandar
Принадлежит:

The present disclosure provides novel methods for microbiological sample preparation from patients that yield high quality input material for antimicrobial susceptibility testing (AST) without the need for plate culture. Certain methods of this disclosure comprise multiple separation steps to purify microbial samples for use in ASTs. 133-. (canceled)34. An automated method for preparing a sample suspected of comprising pathogenic microorganisms derived from a patient for antimicrobial susceptibility testing comprising the steps of:a. applying a relative centrifugal force (RCF) of <1000×g to a sample, thereby producing a particulate depleted supernatant;b. lysing at least one patient cell or platelet in the particulate depleted supernatant, thereby producing a patient cell depleted supernatant;c. applying an RCF of >1000×g to the patient cell depleted supernatant, thereby forming a pellet;d. collecting and resuspending the pellet;e. optionally washing the pellet one or more times by repeating steps c and d; andf. assessing a density of microbes in a saline or buffered solution.35. The method of claim 34 , wherein the lysing is done by a lytic reagent including claim 34 , but not limited to claim 34 , saponins claim 34 , tritons claim 34 , tweens claim 34 , ammonium chloride claim 34 , sorbitan esters claim 34 , nonionic polyoxyethylene surfactants.36. (canceled)37. The method of claim 34 , wherein the lytic reagent comprises claim 34 , but is not limited to claim 34 , sodium polyanethole sulfate (SPS) claim 34 , polypropylene glycol (PPG) claim 34 , potassium carbonate claim 34 , potassium bicarbonate claim 34 , N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) claim 34 , ethylenediaminetetraacetic acid (EDTA) claim 34 , sodium chloride.38. The method of claim 34 , wherein the lytic reagent comprises saponin claim 34 , SPS claim 34 , and PPG.39. (canceled)40. The method of claim 34 , wherein the lytic reagent comprises ammonium chloride and potassium bicarbonate and ...

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Номер записи: 92
01-10-2020 дата публикации

METHODS AND SYSTEMS FOR PREPARING AND PRESERVING A BIOLOGICAL SAMPLE

Номер: US20200305416A1
Автор: Kharasch Evan D., Morrissey Jeremiah J., Singamaneni Srikanth, WANG Congzhou
Принадлежит: WASHINGTON UNIVERSITY

Methods and systems for preparing and preserving biological samples are disclosed herein. A method comprises contacting a preserving agent with a biological sample to form a mixture, wherein the preserving agent is selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant, and wherein the biological sample comprises at least one target analyte. A system comprises a preserving agent selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant; and a substrate configured to receive a drop cast mixture of the preserving agent and a biological sample, wherein the biological sample comprises at least one target analyte. 1. A method of preparing a biological sample comprising:contacting a preserving agent with a biological sample to form a mixture, wherein the preserving agent is selected from at least one of a metal-organic framework (MOF) encapsulant or a precursor forming a MOF encapsulant, and wherein the biological sample comprises at least one target analyte.2. The method according to claim 1 , wherein the MOF is selected from the group consisting of a zeolitic imidazolate framework (ZIF) type MOF and a Materials of Institut Lavoisier (MIL) type MOF.3. The method according to claim 1 , wherein the precursor forming the MOF encapsulant comprises 2-methylimidazole and zinc acetate.4. The method according to claim 3 , wherein a molar ratio of 2-methylimidazole to zinc acetate is in a range of from about 4:1 to about 40:1.5. The method according to claim 1 , further comprising drop casting the mixture onto a substrate.6. The method according to claim 5 , wherein the substrate comprises a water insoluble material.7. The method according to claim 5 , further comprising drying the mixture on the substrate.8. The method according to claim 5 , further comprising storing the substrate:at a temperature of from about −20° C. to about 100° C.; andfor at least 1 week, at ...

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Номер записи: 93
09-11-2017 дата публикации

ACID DIGESTION INSTRUMENT AND VESSEL SYSTEM

Номер: US20170320032A1
Автор: Beard Matt, Collins, Hostak Taylor M., JR. Michael J., Lambert Joseph J., Watkins Dwight D., Zawatsky Timothy A.
Принадлежит: CEM CORPORATION

An instrument system for acid digestion is disclosed. The instrument includes a heating block, a reaction vessel formed of a polymer that is resistant to acid and other chemical attack at temperatures above 150° C. and that has a structure (thickness, etc.) sufficient to withstand pressures above atmospheric, a metal sleeve surrounding the polymeric reaction vessel, and an opening in the block that has a cross-section corresponding to the cross-section of the metal sleeve. 1. An instrument system for acid digestion , comprising:a heating block;a reaction vessel formed of a polymer that is resistant to acid and other chemical attack at temperatures above 150° C. and that has a structure sufficient to withstand pressures above atmospheric;a metal sleeve surrounding said polymeric reaction vessel;an opening in said block having a cross-section corresponding to the cross-section of the metal sleeve.2. An instrument system according to wherein said reaction vessel is formed of a fluorinated polymer.3. An instrument system according to wherein said reaction vessel is formed of polytetrafluoroethylene.4. An instrument system according to wherein said metal sleeve is formed of a metal with sufficient heat conductivity to raise the temperature in the reaction vessel above 150° C. when said sleeve is heated above 200° C.5. An instrument system according to wherein said reaction vessel and said sleeve are cylindrical and the cross-section of said opening in said heating block is cylindrical.6. An instrument system according to wherein said heating block is formed of a material that is resistant to acid attack and that provides rapid heat transfer to said sleeve when said sleeve is in said corresponding opening in said heating block.7. An instrument system according to wherein said heating block is graphite with a plurality of said openings for concurrently receiving and heating a plurality of sleeved vessels.8. The combination of:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1 ...

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Номер записи: 94
09-11-2017 дата публикации

INDEPENDANT HEATING OF SAMPLES IN A SAMPLE HOLDER

Номер: US20170320062A1
Автор: DEBAN Ramin, FEILDERS George, GAUTHIER Jules, GUAY Raymond, ROSS Arthur
Принадлежит:

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample. 1. A method for heating a sample material in a sample holder , the method comprising:receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein;dynamically forming an individual mini microwave cavity around the at least one sample recipient inside the heating chamber; andapplying microwaves generated by at least one microwave generator directly to the sample in the at least one sample recipient within the mini microwave cavity.2. The method of claim 1 , wherein dynamically forming the mini microwave cavities comprises mating a set of first partial mini cavities with a set of complementary second partial mini cavities.3. The method of claim 2 , wherein the set of first partial mini cavities are in the heating chamber and the set of complementary second partial mini cavities are on the sample holder.4. The method of claim 1 , further comprising mechanically positioning the sample holder in the heating chamber to align the set of first partial mini cavities with the set of complementary second partial mini cavities.5. The method of claim 1 , wherein dynamically forming an individual mini microwave cavity comprises forming a plurality of individual mini microwave cavities around each one of a plurality of sample recipients in the sample holder.6. The method of claim 5 , wherein applying microwaves comprises applying microwaves generated using at least two separate microwave generators.7. The method of claim 6 , wherein applying microwaves ...

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Номер записи: 95
01-10-2020 дата публикации

CONTAINER SET AND SAMPLE PREPARATION METHOD USING SAME

Номер: US20200306748A1
Автор: IWAMOTO Noriko, Shimada Takashi, Takanashi Megumi
Принадлежит: SHIMADZU CORPORATION

A container set including: a filter unit; and a closing unit that is attachable to and detachable from the filter unit. The filter unit includes: a housing having a sample introduction opening provided at one end, and a liquid discharging opening provided at the second end; and a filter fixed to the housing. The housing has a first joining part, and the closing unit has a second joining part configured to be joinable with the first joining part. The first joining part and the second joining part are configured such that the liquid discharging opening is spatially closed in a state in which the two joining parts are joined together. A space between the closing unit and the filter is made into a positive pressure relative to a pressure in the sample holding space by pushing the filter unit and the closing unit such that the distance therebetween is shortened. 1. A container set comprising:a filter unit; anda closing unit that is attachable to and detachable from the filter unit, wherein a housing having a first end including a closable sample introducing opening formed therein and a second end including a liquid discharging opening formed therein; and', 'a filter fixed to the housing,, 'the filter unit includesthe sample introducing opening is configured to allow solids and liquids to be therethrough introduced into a sample holding space surrounded by the housing and the filter,the liquid discharging opening is configured to allow a filtrate transmitted through the filter to be therethrough discharged,the housing of the filter unit has a first joining portion,the closing unit has a second joining portion that is joinable to the first joining portion,the first joining portion of the filter unit and the second joining portion of the closing unit are configured such that the first and second joining portions are joinable to each other to produce an airtight state to close the liquid discharging opening spatially by the closing unit, and such that the filter unit and the ...

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Номер записи: 96
17-10-2019 дата публикации

Device and Method for Chemical Analysis

Номер: US20190317081A1
Автор: Abedi Mehdi, Fotouhi Bahram, Fotouhi Mohammed, Greenfield Edward Alvin, Javaherian Kashayar, Mollaaghababa Reza, Nawana Namal, Taslim Mohammad E.
Принадлежит:

A processing and detection system for detecting presence of at least one gluten protein in a food sample comprises a food processor including: a reservoir containing a process liquid for processing the food sample; a body that comprises a chamber configured to receive the food sample; and a pressing surface configured to press on the reservoir to cause the process liquid to exit the reservoir and mix with the food sample, thereby generating a processed food liquid; and an exit port configured to conduct the processed food liquid out of the food processor; and a cartridge including: at least one sensor configured to receive the processed food liquid and to generate an electrical signal in response to interaction with the at least one gluten protein in the processed food liquid, and an analyzer in electrical communication with the at least one sensor for detecting the electrical signal and determining the presence of the at least one gluten protein in the food sample based on the detected electrical signal. 120.-. (canceled)21. A sensor for detecting an analyte in a sample , comprising:a graphene layer,a plurality of antibodies coupled to said graphene layer, said antibodies exhibiting specific binding to said analyte,a reference electrode disposed in vicinity of said graphene layer,an AC voltage source for applying an AC voltage to said reference electrode so as to generate a time-varying electric field at or near vicinity of said graphene surface, anda plurality of electrical conductors electrically coupled to said functionalized graphene layer for measuring an electrical property of said functionalized graphene layer.22. The sensor of claim 21 , wherein said AC voltage source is configured to apply an AC voltage with a frequency in a range of about 1 kHz to about 1 MHz to said reference electrode.23. The sensor of claim 22 , wherein said applied AC voltage has an amplitude in a range of about 1 millivolt to about 3 volts.24. The sensor of claim 21 , wherein said ...

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Номер записи: 97
24-11-2016 дата публикации

A SINGLE REACTOR FOR SIMPLIFIED SAMPLE PREPARATION WORKFLOWS

Номер: US20160341704A1
Автор: "OGRADY John Patrick", ELLIS Bob Harold, HEROLD Nicholas Brian, MEYER Kevin Wayne, POE Derrick Nathaniel
Принадлежит:

This disclosure provides a single reactor that accommodates an affinity selector to separate analytes of interest, and an enzyme reactor that digest the analyte to suitable peptides for mass spectrometry. The single reactor formats described herein accommodate workflows wherein separation precedes digestion as well as workflows wherein digestion precedes separation selection. 1. A single reactor wherein said single reactor performs both affinity purification and enzymatic digestion of protein samples , comprising:an affinity selector, wherein said affinity selector retains and purifies at least one analyte of interest through physical properties of said analyte of interest; and an enzyme reactor, wherein said enzyme is kept relatively inactive during said affinity selection.2. The single reactor of claim 1 , wherein said affinity selector and said enzyme are immobilized in said single reactor.3. The single reactor of claim 1 , wherein said affinity selector and said enzyme reactor are partitioned within said single reactor with controlled access to each other.4. The single reactor according claim 1 , wherein said enzyme is kept relatively inactive during affinity purification steps by means of operation at reduce temperatures claim 1 , buffer composition reversible inhibitors claim 1 , differences in pH claim 1 , compartmentalization or combinations thereof.5. The single reactor according to a claim 1 , wherein said affinity selector is comprised of protein A claim 1 , protein G claim 1 , protein L claim 1 , antibodies claim 1 , antibody fragments claim 1 , biotin claim 1 , avidin claim 1 , hydrophobic materials claim 1 , hydrophilic materials claim 1 , ionic materials claim 1 , lectins claim 1 , metals claim 1 , metal oxides claim 1 , lipid binding proteins claim 1 , chelators claim 1 , phage display proteins claim 1 , natural receptors claim 1 , peptides claim 1 , synthetic affinity reagents claim 1 , boronic acid claim 1 , DNA claim 1 , RNA claim 1 , PNA claim 1 ...

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Номер записи: 98
15-11-2018 дата публикации

PREPARATION OF BIOLOGICAL CELLS ON MASS SPECTROMETRIC SAMPLE SUPPORTS FOR DESORBING IONIZATION

Номер: US20180328822A1
Автор: Sparbier Katrin, WEGEMANN Beatrix
Принадлежит:

The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution. 1. A method for the preparation of proteins from samples of unpurified biological cells on a sample support for the mass spectrometric determination of cellular properties , comprising the steps:providing the biological cells on a sample spot of the sample support,disrupting the cells on the sample spot using at least one of solvents and acids, whereby cell proteins separate from complexes, migrate out of the disrupted cells and are adhesively bonded to the sample spot,washing the cell proteins on the sample spot with buffer solution, andpreparing the washed cell proteins for a subsequent desorbing ionization.2. The method according to claim 1 , wherein the washing step is conducted with a static buffer solution.3. The method according to claim 2 , wherein claim 2 , for the washing step claim 2 , several microliters of an aqueous solution are applied to the sample spot claim 2 , remain there for a predetermined period of time and are then removed.4. The method according to claim 1 , wherein the preparation for the ...

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Номер записи: 99
01-12-2016 дата публикации

Oxidized lipid detection

Номер: US20160349277A1
Автор: Gerald STUBIGER, Omar BELGACEM
Принадлежит: Kratos Analytical Ltd

The present invention is concerned with a method of extracting oxidized lipids from a lipid solution, the method comprising (a) a derivatisation step, comprising contacting a derivatisation agent with the lipid solution such that aldehydic oxidized lipids and/or α,β-unsaturated oxidised lipids, if present in the lipid solution, are derivatised to include an anionic group, and (b) an oxidised lipid capture step, in which nanoparticles are contacted with the lipid solution, wherein the nanoparticles capture anionic-group containing oxidised lipids. The invention also includes a method of extracting aldehydic oxidized phospholipids from a lipid solution, the method comprising (a) a derivatisation step, comprising introduction of a anionic group to aldehydic oxidized lipids and/or α,β-unsaturated oxidised lipids in the lipid solution, and (b) an oxidised lipid capture step, in which nanoparticles are contacted with the lipid solution, wherein the nanoparticles bind anionic-group containing oxidised lipids.

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Номер записи: 100