Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год

Применить Всего найдено 8384. Отображено 100.
05-01-2012 дата публикации

Sampling devices and methods for concentrating microorganisms

Номер: US20120003626A1
Принадлежит: Individual

The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.

Подробнее
27-11-2015 дата публикации

Устройство сушки образцов угля с внутренней циркуляцией горячего проникающего воздуха

Номер: RU0000157332U1

1. Устройство для сушки образцов угля с внутренней циркуляцией горячего проникающего воздуха, включающее коробчатый корпус (1), блок управления (4) и встроенный вентилятор (2), разделительную пластину (3), газовый нагреватель (6), расположенные в коробчатом корпусе (1), где разделительная пластина (3) делит коробчатый корпус (1) на два отсека; вентилятор (2) находится в одном из отсеков; несколько пластинок для образцов угля (5) предусмотрены на разделительной пластине (3); на нижней части каждой пластинки для образцов угля (5) предусмотрены несколько воздушных отверстий (51); и емкость (7) для сбора просыпанных образцов угля располагается под каждой пластинкой для образцов угля (5).2. Устройство для сушки образцов угля с внутренней циркуляцией горячего проникающего воздуха по п. 1, отличающееся тем, что обратный воздуховод (8) расположен на одной стороне указанного корпуса (1); указанный вентилятор (2), являющийся вытяжным, расположен в верхнем отсеке; воздуховыпускное отверстие указанного вентилятора (2) соединено с одним концом указанного обратного воздуховода (8); а другой конец указанного обратного воздуховода (8) соединен с нижним отсеком; а также указанный газовый нагреватель (6) расположен в обратном воздуховоде (8).3. Устройство для сушки образцов угля с внутренней циркуляцией горячего проникающего воздуха по п. 2, отличающееся тем, чтоанемограф (9) для определения скорости потока горячего воздуха расположен в указанном обратном воздуховоде (8); указанный блок управления (4) соединен с вентилятором (2) посредством конвертера (10); указанный конвертер (4) сравнивает сигнал скорости воздушного потока, полученный анемографом (9) с заданно РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 157 332 U1 (51) МПК G01N 1/28 (2006.01) F26B 3/04 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ТИТУЛЬНЫЙ (21)(22) Заявка: ЛИСТ ОПИСАНИЯ ПОЛЕЗНОЙ МОДЕЛИ К ПАТЕНТУ 2015102061/05, 17.05.2013 (24) Дата начала отсчета срока действия патента: 17.05.2013 (72) Автор(ы): ЖУ ...

Подробнее
24-05-2012 дата публикации

Device and process for isolating and cultivating live cells on a filter or extracting their genetic material

Номер: US20120129164A1
Автор: Yvon Cayre
Принадлежит: SCREENCELL

The process for isolating live cells on a filter or extracting their genetic material. The process comprises the steps of attaching, at least temporarily, a filter to a lower opening of a compartment having, in addition, an air inlet; inserting into the compartment a liquid carrying the cells; and attaching, in an impermeable manner, a needle, at least temporarily, to the compartment opening, the filter being positioned between the needle and the interior volume of the compartment. The process further comprises the steps of perforation, with the needle, of a plug of a vacuum tube with negative pressure relative to ambient pressure; and aspiration, by means of negative pressure from the vacuum tube, of the liquid through the filter, the filter retaining the cells.

Подробнее
14-06-2012 дата публикации

Sample Collection System And Method

Номер: US20120144897A1
Принадлежит: Horizon Technology Inc

An apparatus or method for removing water and concentrating an analyte in solution, wherein the concentrated analyte sample is delivered directly to a vial, such as an autosampler vial that is capable of use in a gas chromatography autosampler.

Подробнее
02-08-2012 дата публикации

Sampling and rejection device

Номер: US20120192949A1
Автор: Kevin Petzoldt
Принадлежит: Individual

A sampling and rejection device for a boiler or steam generating system is described. The sampling and rejection device receives the condensate or fluid and allows a volume of the condensate to liquefy or the fluid to build up in the interior of the sampling and rejection device. One or more conductivity, pH, and temperature sensors or probes are positioned in the sampling and rejection device to measure the condensate. The sampling and rejection device includes a collection vessel to hold and temporarily store the condensate. The sampling and rejection device includes an outlet or a return line (to a central boiler) and a drain line. If the sensor measures undesirable conductivity, pH, or temperature in the condensate in the collection vessel, then a valve to the drain line is opened and the condensate is rejected.

Подробнее
02-08-2012 дата публикации

Process analyzer

Номер: US20120195799A1
Принадлежит: HACH LANGE GMBH

A process analyzer for detection of an analyte in a liquid under analysis includes a base module and an exchangeable cartridge module. The exchangeable cartridge module comprises a sample taking device comprising a membrane configured to obtain a sample from the liquid under analysis. A first pump mechanism is configured to pump the sample away from the sample taking device. A second pump mechanism is configured to introduce a reagent into the sample. A measuring section is configured to perform a quantitative detection of the analyte in the sample. A degassing device is arranged downstream of the first pump mechanism and the second pump mechanism. The degassing device is configured to degas the sample.

Подробнее
09-08-2012 дата публикации

Removable final scrubber tube

Номер: US20120198690A1
Принадлежит: Leco Corp

A final scrubber in the inert carrier gas flow path of an elemental analyzer includes a manifold with valves for selectively bypassing a quick disconnect final scrubber housing that includes a filter tube and sealed gas fittings. The housing includes alignment members and a latch for positioning and locking the housing onto and in sealed engagement with the instrument's manifold. A switch detects the presence of the housing, and a control circuit controls valves to direct the inert gas flow though the filter tube or bypass the filter tube when the housing is removed. With this system, the final scrubber can be removed and replaced quickly without the use of tools while the carrier gas continues to flow though the furnace without interruption. Also, the valves can be closed to allow for segmented leak detection of the instruments gas flow path.

Подробнее
25-10-2012 дата публикации

Microfluidic system and method for automated processing of particles from biological fluid

Номер: US20120270331A1
Принадлежит: Individual

A microfluidic system for automatically depleting particles not of interest from a biological sample, comprising: a sampling module configured to receive the sample; and one or more microfluidic protein and nucleic acid depletion modules fluidically coupled to the sampling module and comprising binding agents configured to selectively bind to abundant plasma proteins or nucleic acids. A method for automatically depleting particles not of interest from a sample, comprising: receiving the sample; subjecting the sample to a force that separates at least a portion of the particles not of interest from the sample, thereby isolating at least a portion of the target component; passing the isolated target copmonent into a chamber; circulating the isolated target component in the chamber; and selectively capturing proteins or nucleic acids with binding agents within the chamber.

Подробнее
06-12-2012 дата публикации

Particle Analysis in an Acoustic Cytometer

Номер: US20120304749A1
Принадлежит: Los Alamos National Security LLC

The present invention is a method and apparatus for acoustically manipulating one or more particles.

Подробнее
06-12-2012 дата публикации

Float-Sink Method and Apparatus to Determine Beneficiation Prospects of Minerals

Номер: US20120305453A1
Принадлежит: Tata Steel Ltd

A method for separating particles of different specific gravity from a sized ore feed, wherein the ore can be coal, metallic, non-metallic and mineral ores. The method particles from the ore are sieved to obtain size fractions of different particle size ranges, after which a load of particles of a size fraction are placed in a container and fluidized by passing a fluid flow through the load of particles. By lowering the flow velocity of the fluid through the load the particles are deposited in the container in one or more layers depending on the specific gravity of each of the particles, after which the deposited particles are separated in portions of different specific gravity. The portions represent different ore content of the particles in each portion on basis of which the theoretical yield of the ore can be determined. The invention provides an apparatus to carry out the method.

Подробнее
13-12-2012 дата публикации

Assembly and method for the filtration of a liquid and use in microscopy

Номер: US20120315664A1
Принадлежит: SIEMENS AG

An assembly and method are disclosed for the filtration of a liquid and the use thereof, wherein a supporting body is designed in a recess of a carrier and a filter membrane lies flat on the supporting body. The filter membrane and the supporting body are designed to be permeable to liquids and thus serve as filters, in particular for filtering tumor cells from blood. The carrier can having standard shapes of an object carrier for microscopy and the filtration residue on the filter membrane can be easily handled and examined in the microscope. As a result of the filter membrane lying level on the supporting body, the filtration residue can be particularly well examined microscopically.

Подробнее
20-12-2012 дата публикации

System and method for preparing samples

Номер: US20120322052A1
Принадлежит: 3M Innovative Properties Co

A system and method for preparing samples for analyte testing. The sample preparation system can include a freestanding receptacle. The method can include providing a liquid composition comprising a source and a diluent, and positioning the liquid composition in a reservoir defined by the freestanding receptacle. The method can further include filtering the liquid composition to form a filtrate comprising an analyte of interest, removing at least a portion of the filtrate from the sample preparation system to form a sample, and analyzing the sample for the analyte of interest.

Подробнее
03-01-2013 дата публикации

Apparatus and method for processing biological material

Номер: US20130005032A1
Принадлежит: Baxter International Inc

The application discloses an apparatus and method for processing biological material, including a suspension of cells.

Подробнее
07-02-2013 дата публикации

Method for the extraction and detection of fat-soluble components from biological materials

Номер: US20130034873A1
Автор: Florian J. Schweigert
Принадлежит: DSM IP ASSETS BV

The present invention relates to a method for the analysis of fat-soluble components, in particular dyes, from biological materials, in particular lipid rich foodstuffs, having an enrichment of the components and subsequent analysis. The method comprises a combination of extraction and separation steps and a subsequent analysis step. The invention further relates to an analytical kit and analytical equipment for carrying out the method. The method according to the invention is composed of a plurality of steps. The critical steps which are essential and characterize the invention are: 1. Pre-treatment of the sample to remove lipids. 2. Extracting the dyes into an extraction mixture by a specific solvent or solvent mixture. 3. Destruction of the oxidative sensible ingredients such as carotenoids prior to the final detection and quantification by eye or by optical enhancement.

Подробнее
07-02-2013 дата публикации

Chamber free nanoreactor system

Номер: US20130034880A1
Автор: Mark F. Oldham
Принадлежит: Individual

Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.

Подробнее
21-03-2013 дата публикации

Microfluidic device

Номер: US20130071304A1
Автор: Byung Hee Jeon
Принадлежит: Cytogen Inc

A micro-fluidic device includes a filter cabinet, a first filtering unit and a second filtering unit. The filter cabinet includes a first path and a second path branched from the first path. The first path and the second path are formed within the filter cabinet so that a sample containing different kinds of targets can flow through the first path and the second path. The first filtering unit is installed in an upstream portion of the first path to filter the different kinds of targets from the sample, the first filtering unit configured to guide the different kinds of targets toward the second path. The second filtering unit is installed in the second path to receive the different kinds of targets from the first filtering unit and to filter the different kinds of targets on a size-by-size basis.

Подробнее
18-07-2013 дата публикации

Box producing apparatus, inspection unit, and print register control method for a box producing apparatus

Номер: US20130184134A1
Принадлежит: Futec Inc

An box producing apparatus is disclosed, including a camera that obtains an image of a printed face which is printed on a box material sheet by the printing section; and a register correction control unit that compares a specific picture in a master image of a sample for the print, and a picture in a region specified according to the specific picture in the image of the printed face obtained by the camera to determine an amount of register displacement in each of the print units, and controls a register adjustment mechanism provided in each of the print units based on the amount of register displacement to correct a register of the print.

Подробнее
24-10-2013 дата публикации

Methods and apparatus for particle introduction and recovery

Номер: US20130277223A1
Принадлежит: University of British Columbia

Particles may be injected into a matrix for concentration by scodaphoresis using a quadrupole injection field. Particles may be injected from two or more sample chambers simultaneously. Particle injection may be performed simultaneously with performing scodaphoresis. In some embodiments the particles are concentrated into a well containing fluid. The well can extend out of a plane of the matrix. Altering the relative phases of components of a scodaphoresis field permits concentration of selected particles and exclusion of other particles. Scodaphoresis methods may be applied to DNA, other bio-molecules and other particles.

Подробнее
31-10-2013 дата публикации

Cell collecting device

Номер: US20130288360A1
Принадлежит: Cytogen Inc

A cell collecting device is configured to collect target cells from a fluid sample such as blood or physiological fluid. The cell collecting device includes a first filter having a plurality of first pores formed into a size that enables target cells contained in a fluid sample to pass through the first pores, and a second filter having a plurality of second pores formed into a size that enables the target cells contained to pass through the second pores. The second filter is arranged below the first filter in such a position as to filter the target cells.

Подробнее
14-11-2013 дата публикации

In situ marine sample collection system and methods

Номер: US20130298702A1
Принадлежит: Woods Hole Oceanographic Institute WHOI

According to one aspect, the invention relates to a marine sample collection system adapted for in situ use. The system includes a first filter head and a second filter head for filtering material of interest from ambient marine fluid passing therethrough and a respective filter flow meter disposed downstream of each of the filter heads for measuring volumetric flow through each filter head. The system also includes a pump downstream of the filter heads for inducing flow through the filter heads and an outlet flow meter disposed downstream of the pump for measuring volumetric flow through the pump. An optional controller compares a sum of an output of the flow meters associated with the first and second filter heads and an output of the outlet flow meter to determine if there is leakage in the system.

Подробнее
28-11-2013 дата публикации

Method and apparatus for obtaining heavy oil samples from a reservoir sample

Номер: US20130312543A1
Принадлежит: Gushor Inc

The invention relates to an apparatus and method to obtain a bitumen or heavy oil sample from an oil reservoir sample, such as a core sample, to enable measurement of physical properties such as viscosity, API gravity, or chemical properties such as sulphur content of the obtained bitumen or heavy oil sample. The analyses performed on the samples obtained in accordance with the invention are effective in assisting oil field operators in making timely drilling and production decisions at the oil reservoir or for routine laboratory extraction of oils and bitumens. The invention also permits the collection of samples from simulated thermal recovery operations and also allows the collection of bitumens and oils for online analysis of live oil physical properties.

Подробнее
09-01-2014 дата публикации

Automated systems and methods for detection of chemical compounds

Номер: US20140007710A1
Автор: Paul Danilchik
Принадлежит: Brooks Rand Inc

In accordance with one embodiment of the present disclosure, a method for processing a liquid test sample includes using a needle assembly to introduce a first flow of gas through an inlet in the needle assembly into a container containing a liquid test sample and to provide an outlet from the container through the needle assembly, separating at least one volatile component from the sample in a gas and liquid separator using the first flow of gas, adsorbing the at least one volatile component onto a trapping material to provide at least one adsorbed component, and releasing the at least one adsorbed component from the trapping material to provide at least one released component.

Подробнее
23-01-2014 дата публикации

Method and apparatus for precise seletion and extraction of a focused component in isoelectric focusing performed in micro-channels

Номер: US20140021053A1
Принадлежит: Individual

An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.

Подробнее
30-01-2014 дата публикации

Apparatus for detecting tumor cells

Номер: US20140030799A1
Автор: Chris C. Yu, He Yu, Xuedong Du
Принадлежит: Anpac Bio Medical Science Co Ltd

Among others, the present invention provides apparatus for detecting circulating tumor cells, comprising a system delivery biological subject and a probing and detecting device, wherein the probing and detecting device includes a first micro-device and a first substrate supporting the first micro-device, the first micro-device contacts a biologic material to be detected and is capable of measuring at the microscopic level an electrical, magnetic, electromagnetic, thermal, optical, acoustical, biological, chemical, electro-mechanical, electro-chemical, electro-optical, electro-thermal, electro-chemical-mechanical, bio-chemical, bio-mechanical, bio-optical, bio-thermal, bio-physical, bio-electro-mechanical, bio-electro-chemical, bio-electro-optical, bio-electro-thermal, bio-mechanical-optical, bio-mechanical thermal, bio-thermal-optical, bio-electro-chemical-optical, bio-electro-mechanical-optical, bio-electro-thermal-optical, bio-electro-chemical-mechanical, physical or mechanical property, or a combination thereof, of the biologic subject.

Подробнее
20-03-2014 дата публикации

Monolithic silicone and method of separation, purification and concentration therewith

Номер: US20140076070A1
Принадлежит: GL Science Inc, KYOTO UNIVERSITY

The present invention provides a monolithic silicone in the form of an aerogel or a xerogel having flexibility and capable of dissolving molecules of a substance. This silicone monolithic body having continuous through passages is synthesized by copolymerizing starting materials of both a bifunctional alkoxysilane and a trifunctional alkoxysilane or tri- or higher functional alkoxysilanes through a sol-gel reaction for forming a Si—O network while causing phase separation.

Подробнее
20-03-2014 дата публикации

High Definition Nanomaterials

Номер: US20140079601A1

A microfluidic device for manipulating particles can include a substrate and one or more obstacles, each obstacle comprising a plurality of aligned nanostructures including a plurality of nanoparticles or a plurality of polymer layers, or a combination thereof. The obstacle on a substrate can be forests with intra-carbon nanotube spacing ranging between 5-100 nm for isolation of particles such as very small viruses and proteins.

Подробнее
07-01-2021 дата публикации

DISPOSABLE POLYMER-STRUCTURED FILTER

Номер: US20210001249A1
Автор: Cai Jianjian, Liu Xiaogao
Принадлежит: Chemrus Inc.

The disposable polymer-structured filtering kit includes a disposable, polymer-structured filtering funnel with a stem having a distal tip. A flow discharge end is formed at the distal tip. Preferably, a polymer fritted filter disc is positioned in the funnel, providing filtering for liquids passing therethrough. The kit preferably also includes a glass vacuum take-off adapter having a port for connecting to a vacuum source for providing negative pressure. The adapter securely and snuggly receives the funnel and maintains position of the distal tip thereof with the flow discharge end below the port, thus preventing contaminants from entering the adapter. A reusable, glass round bottle flask or a disposable vial receives the adapter and the stem of the funnel. The funnel and fritted disc are formed from disposable materials, thus removing the necessity of cleaning them following use. The adapter is reusable, since no contaminants come in contact therewith during filtering. 120-. (canceled)21. A disposable polymer-structured filtering kit , comprising:a disposable funnel having an upper portion and a detachable lower portion, the lower portion comprising a base, a top end of said base having a concavity and the lower end thereof defining a stem having a flow discharge end positioned at a distal tip thereof;a filter disc received within the concavity of the lower portion of the funnel, the filtering funnel being constructed of a polymer;a vacuum take-off adapter having a port adapted for connection to an external vacuum source, the vacuum-take off adapter having a body portion with a funnel engaging end and a flask or vial engaging end, wherein the funnel engaging end of the vacuum take-off adapter receives the lower portion of the filtering funnel, and wherein the vacuum take-off adapter has a funnel joint that receives the stem of the funnel; anda bottle flask or vial releasably connected to the adapter at the flask or vial engaging end; whereinthe stem passes ...

Подробнее
07-01-2021 дата публикации

Device for analysis of cellular motility

Номер: US20210001335A1
Принадлежит: MOTILITYCOUNT APS

A mesoscale fluidic system comprises a substrate having a sample chamber and an analysis chamber. The sample chamber comprises a cell permeable filter defining a sample application compartment and a conditioning medium compartment. The sample chamber has a sample inlet port in the sample application compartment. The analysis chamber has an entry port and an exit port. The conditioning medium compartment is in fluid communication with the entry port of the analysis chamber via a channel. The sample application compartment is below the cell permeable filter and the conditioning medium compartment is above the cell permeable filter. The mesoscale fluidic system is suited for analysing cellular motility in a sample. Also disclosed is a method of estimating the quantity of motile cells in a sample and a method of extracting motile cells from non-motile cells.

Подробнее
04-01-2018 дата публикации

DEVICE FOR SOLID PHASE EXTRACTION AND METHOD FOR USE THEREOF

Номер: US20180001229A1
Принадлежит:

Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention. 1. A separation device comprising two or more sorbents wherein:the matrix interferences retained on one of the sorbents is substantially removed by the other sorbents as compared to a separation device comprising only one sorbent;the flowrate of the sorbents is substantially improved as compared to a separation device comprising only one sorbent; orthe backpres sure of the sorbents is substantially reduced as compared to a separation device comprising only one sorbent.2. The separation device of claim 1 , wherein at least one sorbent contains at least one hydrophobic component and at least one hydrophilic component.3. The separation device of claim 2 , wherein the sorbent that contains at least one hydrophobic component and at least one hydrophilic component is water-wettable.4. The separation device of claim 1 , wherein at least one of the sorbents has retention for analytes having a log P of 0.05 or greater in aqueous solutions.5. The separation device of claim 4 , wherein the remainder of the sorbents claim 4 , in total claim 4 , have a specific affinity for matrix interferences in greater than 40% organic solvent solutions.6. The separation device of claim 1 , wherein at least one sorbent has a specific affinity to phospholipids.7. The separation device of claim 1 , wherein the sorbents are selected from the group consisting of a silica claim 1 , a modified silica claim 1 , an alumina claim 1 , an modified alumina claim ...

Подробнее
04-01-2018 дата публикации

DEVICES FOR SEPARATION OF PARTICULATES, ASSOCIATED METHODS AND SYSTEMS

Номер: US20180001231A1
Принадлежит:

A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber. 1. A separation device for separating particulates dispersed in a base fluid , the device comprising:a fluid inlet;a fluid outlet;a first microchannel disposed between the fluid inlet and the fluid outlet;a microporous body defining at least a portion of the first microchannel; anda mesofluidic collection chamber on one side of the microporous body;wherein the particulates dispersed in the base fluid traverse through the first microchannel under an influence of a force field, andwherein the particulates delaminate from the base fluid in the first microchannel and trace a fluidic expansion while entering to the mesofluidic collection chamber, and wherein at least a portion of the particulates in at least a portion of the base fluid are collected in the mesofluidic collection chamber.2. The device of claim 1 , further comprising a second microchannel having a second fluid outlet disposed at the second side of the mesofluidic collection chamber claim 1 , such that the fluid inlet claim 1 , first microchannel claim 1 , second microchannel claim 1 , and second fluid outlet are in a fluidic communication via the mesofluidic collection chamber claim 1 , and wherein the second microchannel has a length lin a range from about 5 millimeters to about 100 millimeters and a height h.3. The device of claim 2 , further comprising an input ...

Подробнее
03-01-2019 дата публикации

Filtration device with multiple post-filtration orientations

Номер: US20190001323A1
Принадлежит: Boston Scientific Scimed Inc

A cell filtration assembly adapted to capture cells from a biological sample during centrifugation includes a centrifugation tube, a cell collection device adapted to be secured within a tapered end of the centrifugation tube and a funnel structure adapted to direct fluid into the cell collection device. The cell collection device includes a rectilinear structure that is adapted to permit fluid to flow through the rectilinear structure and a cell capture surface that is secured relative to the rectilinear structure. The cell collection device may be sectioned in either a horizontal or vertical orientation.

Подробнее
04-01-2018 дата публикации

Single-Platform Integrated Aquatic Species and Habitat Sampling System

Номер: US20180001974A1
Автор: Joseph Merz

Low or no disturbance sampling can be accomplished such as through a single-platform aquatic species and habitat sampling system with data integration and rapid processing capabilities that can address the need for sampling at variable depths over varied habitats, along with the simultaneous collection of linked physical and biological data. The platform may be based on a 24-36 foot boat, and may include a net mouth opener brace for an adjustable concentrator net and smaller drift net which may be attached to an adjustable sample chamber, perhaps containing variable mesh capture nets as well as cameras, water sampling equipment, and water quality sensors integrated with a fish finder, GPS, and other monitoring and data recording equipment. The depth of the net mouth opener brace and sample chamber may be adjustable using a depth control.

Подробнее
02-01-2020 дата публикации

SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING

Номер: US20200002663A1
Принадлежит:

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. 1. (canceled)2. A lysing apparatus , comprising:a container having at least one chamber to hold a material to be lysed and a lysing particulate material, the chamber having a first opening that provides fluid communication into the chamber from an exterior thereof;an impeller having a number of blades positionable in the chamber of the container; anda micromotor coupled to turn the impeller, at least a portion of the micromotor removably receivable in the first opening of the container to seal the first opening in use.3. The lysing apparatus of wherein the container comprises a second opening positioned above the first opening claim 2 , the second opening provides fluid communication into the chamber from the exterior thereof.4. The lysing apparatus of wherein the container comprises a third opening that provides fluid communication into the chamber from the exterior thereof.5. The lysing apparatus of wherein the first opening is the only opening in the container.6. The lysing apparatus of wherein the micromotor is disposable.7. The lysing apparatus of wherein the micromotor pulsates.8. The lysing apparatus of wherein the micromotor drives the impeller at a rate of greater than 10 claim 2 ,000 RPM.9. The lysing apparatus of wherein the ...

Подробнее
05-01-2017 дата публикации

Volatile Hydrocarbon Separation and Analysis Apparatus and Methods

Номер: US20170003264A1

At least one embodiment of the inventive technology may be described as a method for analyzing a hydrocarbon that comprises volatiles, said method comprising the steps of: segregating said volatiles from said hydrocarbon without oxidizing said hydrocarbon; generating a hydrocarbon residue and segregated hydrocarbon volatiles; and analyzing at least one of said hydrocarbon residue and said segregated hydrocarbon volatiles. The advantageous avoidance of oxidation may be achieved by placing the hydrocarbon under a vacuum, which may also enable the avoidance of cracking of the hydrocarbon while still achieving segregation of volatiles as desired. One other of the several embodiments disclosed and claimed herein may focus more on vacuum transfer and vacuum distillation of hydrocarbon volatiles. These and other methods disclosed herein may be used to achieve improved hydrocarbon analysis results.

Подробнее
05-01-2017 дата публикации

Device and Method for Separating A Fluid Mixture such as Blood

Номер: US20170003270A1
Принадлежит:

A lab-on-chip device for the processing, in particular the separation, of a fluid mixture comprising two immiscible phases (liquid and/or solid), said device comprising a fluid line () which successively includes an inlet reservoir (), a separation channel (), a collection channel () and an outlet (), said separation channel () being designed in a way as to allow a separation of the fluid mixture into said two phases. 118- (canceled)19. A lab-on-chip device for processing a fluid mixture having two immiscible phases , the device comprising:a fluid line which successively includes an inlet reservoir, a separation channel, a collection channel, and an outlet,wherein the separation channel is configured to allow a separation of the fluid mixture into the two immiscible phases.20. The device according to claim 19 , wherein a size of the separation channel is configured to induce sedimentation and separation of the fluid mixture and generation of a purified fluid by capillary action.21. The device according to claim 19 , further comprising:an air bubble actuator configured to, when actuated, generate an air bubble into the fluid line to isolate a defined volume of processed fluid.22. The device according to claim 19 , the device being configured to generate a purified fluid by capillary driven forces within the separation channel.23. The device according to claim 19 , the device configured to generate a purified fluid by sedimentation within the separation channel.24. The device according to claim 19 , wherein dimensions of the separation channel and wettability of surfaces of the separation channel are configured to allow precise control of a filling speed.25. The device according to claim 19 , wherein dimensions of the separation channel and wettability of surfaces of the separation channel are configured to allow precise control of a shape of a front flow of the fluid mixture.26. The device according to claim 19 , further comprising:a metering channel that ...

Подробнее
05-01-2017 дата публикации

MULTI-SITE PARTICLE SENSING SYSTEM

Номер: US20170003283A1
Принадлежит: PURDUE RESEARCH FOUNDATION

A particle sensing system which includes a plurality of micro-lenses which focus light from an unfocused or loosely focused light source onto a corresponding plurality of focus regions on a surface containing plasmonic structures. The absorption of light by the plasmonic structures in the focus regions results in heat dissipation in the plasmonic structures and consequently increases surface temperature in the focus regions. When an electrical field is applied to a sample fluid in contact with the surface, multiple electrothermal flows are induced in the fluid which rapidly transport suspended particles to the focus regions on the surface. The particles can then be captured and/or sensed. 1. A particle sensing system , comprising:a first substrate having a first conductive layer on a first side of the first substrate, and a plurality of microlenses mounted to the first conductive layer;a second substrate having a second conductive layer, the second conducting layer facing the first conductive layer, the second conductive layer having a plurality of light absorbing plasmonic structures; andat least one channel separating the first and second conductive layers and configured to hold a liquid sample;wherein the microlenses are configured to create a plurality of focus regions on the second conductive layer when light is directed through the microlenses.2. The system of claim 1 , further comprising an alternating current (AC) source connected between the first and second conductive layers to induce an electric field in the liquid sample.3. The system of claim 1 , wherein the distance between the microlenses and the second conductive layer is chosen to be equal to a focal length of the microlenses.4. The system of claim 1 , wherein the plurality of microlenses are arranged in arrays on the first conductive layer.5. The system of claim 1 , wherein a thin dielectric spacer layer is placed between the microlenses and the first conductive layer.6. The system of claim 1 , ...

Подробнее
01-01-2015 дата публикации

Spectrometric device for the analysis of environmental and geological samples

Номер: US20150004714A1
Автор: John David HANBY
Принадлежит: HANBY INTERNATIONAL LLC

A system and method for analyzing contaminants such as hydrocarbons in soil and ground water utilizes a reaction device comprising a catalyst encapsulated in a permeable material and processes that device in contact with a contaminant in an analytical device in order to generate a spectrogram indicative of the contaminants in the soil and ground water.

Подробнее
04-01-2018 дата публикации

PROCESS AND DEVICE FOR SEPARATING BIOLOGICAL PARTICLES CONTAINED IN A FLUID BY MEANS OF FILTRATION

Номер: US20180003602A1
Принадлежит:

The invention relates to a method of separating biological particles from the liquid containing same for purification, analysis and optionally diagnostic purposes. The inventive method comprises at least one step involving vertical filtration through a filter having a porosity that is adapted to the type of biological particles to be separated, such that said particles are retained by the filter. The invention is characterised in that: (i) the method involves the use of a filter comprising at least one basic filtration zone, whereby each basic filtration zone has a limited surface area; and (ii) the surface area of each basic filtration zone and the number of basic filtration zones are selected as a function of the type of liquid to be filtered, the type of biological particles to be separated and the volume of liquid to be filtered. 131-. (canceled)32. A process for separating biological particles and the fluid that contains them comprisingsubjecting a fluid containing biological particles to at least one vertical filtration stage through a filter having a porosity suited to the nature of the biological particles to be separated so that said biological particles are retained by the filter, and the fluid passes through the filter the filter comprises at least one elementary filtration area, each elementary filtration area having a limited surface,', 'each elementary filtration area and the number of elementary filtration areas are set according to the nature of the fluid to be filtered, the nature of the biological particles to be separated and the volume of fluid to be filtered,', 'each elementary filtration area has a surface equal to that of a disk with a diameter of between 0.6 cm and 3 cm, and', {'sup': '2', 'the number of elementary filtration areas is set so that the ratio of the volume of fluid filtered and of the filtration surface is less than 40 ml/cm.'}], 'wherein'}33. The process according to claim 32 , wherein each elementary filtration area has a ...

Подробнее
04-01-2018 дата публикации

ANALYTICAL SYSTEM AND METHOD FOR DETECTING VOLATILE ORGANIC COMPOUNDS IN WATER

Номер: US20180003682A1
Принадлежит:

An analytical system and method for detecting volatile organic chemicals in water including a coated SAW detector that provides for improved reduction of moisture at the coating of the SAW detector. A stabilized SAW sensitivity and long lasting calibration is achieved. The analytical system further includes an improved sample vessel and sparger that allow for easy grab sample analysis, while also providing efficient purging of the volatile organic compounds from the water sample. In addition, an improved preconcentrator provides a stabilized sorbent bed. 1. A system for detecting organic compounds in water including:a preconcentrator configured to collect the organic compounds;a gas chromatograph column configured to separate the organic compounds as desorbed from the preconcentrator;a surface acoustic wave detector configured to detect the mass of the organic compounds separated by the gas chromatograph;a housing that houses the preconcentrator, the gas chromatograph column, and the surface acoustic wave detector; anda sample vessel removably attached to the housing configured to contain a water sample from which the organic compounds are purged.2. A system according to claim 1 , wherein the sample vessel is configured to contain a water sample of about 40 mL.3. A system according to claim 1 , wherein a level of the water sample in the sample vessel ranges from about 2.5 inches to about 4 inches claim 1 , and the distance between a fill line of the water sample and the top end of the sample vessel ranges from about 3.5 inches to about 5 inches.4. A system according to claim 1 , wherein the sample vessel includes a retaining member.5. A system according to claim 4 , wherein the retaining member includes threads.6. A system according to claim 4 , wherein the housing includes a complimentary retaining member.7. A system according to claim 6 , wherein the complimentary retaining member is a retaining tube nut.8. A system according to claim 1 , wherein the sample vessel ...

Подробнее
02-01-2020 дата публикации

Centrifugal filtration device and method of capturing and observing fine particles in liquid using the same

Номер: US20200003661A1
Принадлежит: Organo Corp

A centrifugal filtration device is provided. The centrifugal filtration device has filtration membrane that filtrates liquid; cartridge that supports filtration membrane and that forms liquid chamber together with filtration membrane, wherein liquid chamber holds the liquid therein; and rotating member that rotates around rotation center and that supports cartridge such that filtration membrane is positioned outward of liquid chamber with respect to rotation center. Rotating member has a path that is connected to liquid chamber, and at least a part of a liquid contact part of the path that is in contact with the liquid is formed of titanium or a titanium alloy.

Подробнее
02-01-2020 дата публикации

GENERIC PRETREATMENT REAGENTS FOR ANALYTE DETERMINATIONS

Номер: US20200003666A1
Принадлежит: ROCHE DIAGNOSTICS OPERATIONS, INC.

The present disclosure relates to an in vitro method for releasing analytes from a sample involving contacting the sample with (i) a chaotropic agent, (ii) an organic solvent, (iii) a detergent, and (iv) at least one agent providing bicarbonate ions. The present disclosure further relates to a release agent for releasing analytes from a sample having the aforesaid compounds. Moreover, the present disclosure relates to uses, kits, methods and devices related to the method and the release agent of the present disclosure. 1. An in vitro method for releasing analytes from a sample comprising contacting said sample with (i) a chaotropic agent , (ii) an organic solvent , (iii) a detergent , and (iv) at least one agent providing bicarbonate ions.2. The method of claim 1 , wherein the concentration of said chaotropic agent is of from 1 M to 4 M;wherein the concentration of said organic solvent is of from 2% (v/v) to 20% (v/v);wherein the concentration of said detergent is of from 0.2% (w/v) to 20% (w/v); andwherein the concentration of bicarbonate ions formally provided by said at least one agent providing bicarbonate ions is of from 0.1 M to 2 M.3. The method of claim 1 , wherein said chaotropic agent is guanidine hydrochloride and/or urea;wherein said organic solvent is acetonitrile;wherein said detergent is a non-ionic detergent; andwherein said at least one agent providing bicarbonate ions comprises a cyclic carbonate ester.4. The method of claim 1 , wherein said sample is a bodily fluid sample.5. The method of claim 1 , wherein said analytes comprise at least one of a steroid hormone claim 1 , an oligopeptide claim 1 , a polyketide claim 1 , and a vitamin.6. The method of claim 1 , wherein said steroid hormone is at least one of an androgen claim 1 , an estrogen claim 1 , and a progestogen;wherein said oligopeptide is a cyclic oligopeptide antibiotic;wherein said polyketide is a macrolide, tacrolimus, sirolimus, or everolimus; andwherein said vitamin is a vitamin D.7. ...

Подробнее
02-01-2020 дата публикации

Methods for predicting overall and progression free survival in subjects having cancer using circulating cancer associated macrophage-like cells (camls)

Номер: US20200003781A1
Автор: Cha-Mei Tang, Daniel Adams
Принадлежит: Creatv Microtech Inc

Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number arid size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

Подробнее
07-01-2021 дата публикации

READY-TO-EXTRACT PLATFORMS FOR CHEMICAL ANALYSIS AND QUANTIFICATION OF UNKNOWN SAMPLES USING SPIKED MATRIX STANDARDS

Номер: US20210003488A1
Принадлежит: PinPoint Testing, LLC

The present disclosure provides a set of spiked matrix standards comprising: one or more carriers, each carrier comprising: (a) a reference standard analyte in an amount that is different in each carrier; and (b) a blank matrix that is in admixture or in contact with the reference standard analyte. Methods for preparing and quantitating an analyte in a test sample comprises (a) providing one or more test samples to be tested for the presence of the analyte (b) providing a set of spiked matrix standards; (c) processing the standards and test samples and (d) quantitating the amount of analyte in each of the standards and test samples. 1. A set of spiked matrix standards comprising:(a) a reference standard analyte in an amount that is different in each spiked matrix standard level;(b) a blank matrix that is in admixture with the reference standard analyte; and(c) a carrier which houses or contains the blank matrix and reference standard analyte.2. The set of spiked matrix standards according to claim 1 , wherein the carrier contains an internal volume ranging from about 5.0 mL to about 0.1 mL.3. The set of spiked matrix standards according to claim 1 , wherein the blank matrix comprises a solid plant extract claim 1 , corn starch claim 1 , brownie mix claim 1 , pulverized cookie crumbles claim 1 , or a gelatin containing food product.4. The set of spiked matrix standards according to claim 1 , wherein the blank matrix comprises a solid plant extract.5. The set of spiked matrix standards according to claim 4 , wherein the solid plant extract comprises a plant material that has a moisture content ranging from about 2% to about 0.001% claim 4 , and comprises plant tissue selected from at least one plant part comprising leaves claim 4 , stems claim 4 , flowers claim 4 , roots claim 4 , or combinations thereof.6Cannabis sativa, Cannabis indincaCannabis ruderalis.. The set of spiked matrix standards according to claim 4 , wherein the reference standard analyte is at least ...

Подробнее
03-01-2019 дата публикации

Enzymatic sample purification

Номер: US20190003937A1
Принадлежит: Hewlett Packard Development Co LP

An enzymatic purification method involves the introduction of a sample comprising a target analyte and amino acids into a porous matrix of a reaction chamber. The reaction chamber includes first pores and second pores. The first pores contain polypeptide synthesis enzymes that react with the amino acids to form polypeptides. First pores having a first size to be accessible by amino acids but inaccessible by the subsequently formed polypeptides. The second pores have a second size greater than the first size, are in contact with the first pores and form a series extending from within the reaction chamber to a waste chamber. The formed polypeptides are migrated through the series of second pores to the waste chamber. The target analyte of the sample is extracted from the reaction chamber.

Подробнее
03-01-2019 дата публикации

Means and methods for determination of a metabolic state of a plant

Номер: US20190004035A1
Принадлежит: BASF Plant Science Co GmbH

Provided herein is a method for determining a metabolic state of a plant or part thereof comprising a) rapid evaporating a multitude of metabolites of said plant or part thereof; b) determining the amount of at least one metabolite characteristic of said metabolic state; and c) thereby, determining a metabolic state of a plant thereof. Further provided is a method for in vivo determining a metabolite distribution in a plant or part thereof comprising a) in vivo rapid evaporating at least one metabolite of interest in at least a first and a second location of said plant or part thereof; b) determining the amounts of at least one metabolite at said first and a second location; and, c) thereby, in vivo determining metabolite distribution in a plant or part thereof. Moreover, provided are devices, data collections, and uses relating to the aforesaid methods.

Подробнее
20-01-2022 дата публикации

HIGH/HYPERVELOCITY PARTICLE CAPTURE AND ANALYSIS METHOD AND APPARATUS

Номер: US20220018741A1

In various embodiments a capture surface for capturing high velocity and hypervelocity dust and ice particles is provided. In certain embodiments the capture surface is comprised of a soft metal that is chosen to optimize particle capture efficiency, to minimize thermal degradation of chemicals and biochemical in the particles, and to present the captured particles to an analyzer for chemical and biochemical analysis of the particles and their contents. In various embodiments capture chambers comprising one or more such capture surfaces are provided as well as methods of use thereof. 1. A particle capture surface configured for capture of high and/or hyper velocity dust , aerosol , and/or ice particles , wherein said capture surface is comprised of soft metal that maximizes particle capture efficiency , minimizes thermal degradation and shock degradation of chemical and biochemical components in the particles , and said surface is configured to present the captured particles , or components therein , on said surface for direct analysis or to deliver said particles , or component therein , to an analyzer for chemical and/or biochemical analysis of the particles and their component contents.2. The particle capture surface of claim 1 , wherein said surface is configured to deliver said particles to an analyzer for chemical and/or biochemical analysis of the particles and their contents.3. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles.4. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles in high earth orbit.5. The particle capture surface according to any one of - claim 1 , wherein said surface is configured to capture extraterrestrial dust claim 1 , aerosol claim 1 , and/or ice particles at high altitude.6. ...

Подробнее
20-01-2022 дата публикации

BRIDGING LIQUID BETWEEN MICROFLUIDIC ELEMENTS WITHOUT CLOSED CHANNELS

Номер: US20220018744A1
Принадлежит:

According to a first aspect, the present invention is embodied as a method of processing a filtered liquid with a microfluidic device. The method includes positioning a porous filtering medium with respect to the microfluidic device, so as to allow a flow path between the filtering medium and a channel of the microfluidic device. The method further includes introducing a liquid in the porous filtering medium for the liquid to advance along the filtering medium and be filtered by the medium. The method further includes applying compression to the filtering medium to extract a given volume of the filtered liquid from the filtering medium, where the extracted liquid volume reaches said channel via the flow path. The method further includes processing the extracted volume with the microfluidic device. 1. A method of processing a liquid with a microfluidic device , the method comprising:positioning a porous filtering medium with respect to the microfluidic device, so as to allow a flow path between the filtering medium and a channel of the microfluidic device;introducing a liquid in the porous filtering medium for the liquid to advance along and be filtered by the filtering medium;applying compression to the filtering medium to extract a given liquid volume of the filtered liquid from the filtering medium, whereby the extracted liquid volume reaches said channel via the flow path; andprocessing the extracted liquid volume with the microfluidic device.2. The method according to claim 1 , wherein the porous filtering medium is positioned so as to contact one or more of an entity selected from the group consisting of: a holder and the microfluidic device.3. The method according to claim 2 , wherein:the microfluidic device comprises a substrate structured so as to form said channel and a cover layer covering a first portion of a top surface of the structured substrate; andthe porous filtering medium is positioned so as for:a bottom surface of the filtering medium to contact ...

Подробнее
12-01-2017 дата публикации

Apparatus and Methods for Controlling Application of a Substance to a Substrate

Номер: US20170007995A1
Принадлежит: RR Donnelley and Sons Co

Apparatus and methods for controlling application of a substance to a substrate involve the use of a functional agent that helps determine the association of a substance with the substrate. The apparatus and methods may utilize jet technology to apply the functional agent directly to the substrate or to an intermediate surface. The substance may be biological substance or a chemical in nature.

Подробнее
14-01-2021 дата публикации

Cell capture in microfluidic devices

Номер: US20210008554A1
Принадлежит: Astrego Diagnostics AB

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device(1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).

Подробнее
08-01-2015 дата публикации

SPECIMEN CONCENTRATION CONTAINER AND SPECIMEN CONCENTRATING METHOD USING SAME

Номер: US20150011012A1
Принадлежит:

A specimen concentration container contains a specimen-containing liquid mixture, and the liquid mixture is concentrated in the specimen concentration container. The specimen concentration container includes: a tubular container main body including an upper surface on which an upper surface opening portion is formed; an upper surface opening portion communicating with an inside of the container main body; a specimen concentration portion formed at a bottom portion side of the container main body and containing the concentrated liquid mixture; and a specimen lid provided in the container main body and configured to cover the upper surface opening portion. 1. A specimen concentration container which contains a specimen-containing liquid mixture and in which the liquid mixture is concentrated ,the specimen concentration container comprising:a tubular container main body including an upper surface on which a first opening portion is formed;a specimen concentration portion which includes a second opening portion communicating with an inside of the container main body, is formed at a bottom portion side of the container main body, and contains the concentrated liquid mixture; anda specimen lid provided in the container main body and configured to cover the second opening portion.2. The specimen concentration container according to claim 1 , wherein:an inner diameter of the second opening portion is smaller than that of the container main body; andthe specimen concentration portion has a bottomed tubular shape including an upper portion on which the second opening portion is formed.3. The specimen concentration container according to claim 1 , wherein when the liquid mixture is concentrated claim 1 , and a liquid surface of the liquid mixture becomes lower than the second opening portion of the specimen concentration portion claim 1 , the specimen lid covers the second opening portion.4. The specimen concentration container according to claim 1 , wherein the specimen lid ...

Подробнее
09-01-2020 дата публикации

Density Phase Separation Device

Номер: US20200009552A1
Принадлежит:

A mechanical separator for separating a fluid sample into first and second phases within a collection container is disclosed. The mechanical separator may have a separator body having a through-hole defined therein, with the through-hole adapted for allowing fluid to pass therethrough. The separator body includes a float, having a first density, and a ballast, having a second density greater than the first density. A portion of the float is connected to a portion of the ballast. Optionally, the float may include a first extended tab adjacent a first opening of the through-hole and a second extended tab adjacent the second opening of the through-hole. In certain configurations, the separator body also includes an extended tab band disposed about an outer surface of the float. The separator body may also include an engagement band circumferentially disposed about at least a portion of the separator body. 1. A mechanical separator for separating a fluid sample into first and second phases within a collection container , comprising: a float, having a first density; and', 'a ballast having a second density greater than the first density and comprising an attachment portion connected to the float and at least one projection extending from the attachment portion., 'a separator body having a through-hole defined therein, the through-hole adapted for allowing fluid to pass therethrough, the separator body comprising2. The mechanical separator of claim 1 , wherein the ballast has at least two projections extending from the attachment portion.3. The mechanical separator of claim 1 , wherein at least a portion of the separator body is adapted to resiliently deform upon application of rotational force.4. The mechanical separator of claim 1 , wherein the separator body has a generally spheroid shape and an outer surface of the float at an interface between the float and the ballast is offset from an outer surface of the ballast at the interface.5. The mechanical separator of ...

Подробнее
12-01-2017 дата публикации

SYSTEMS AND METHODS FOR DETECTING AN ANALYTE OF INTEREST IN A SAMPLE USING MICROSTRUCTURED SURFACES

Номер: US20170010199A1
Принадлежит:

Method for detecting an analyte of interest in a sample. The method can include providing a container comprising a microstructured surface, and centrifuging the container toward the microstructured surface to form a sediment and a supernatant of the sample. Following centrifugation, the container can be inverted to decant at least a portion of the supernatant of the sample from the second portion, such that a concentrate (e.g., comprising the sediment) of the sample is retained in the microstructured surface. The concentrate can then be interrogated in the microstructured surface for the analyte of interest. In some embodiments, at least a portion of the second portion can be substantially transparent, such that the concentrate can be interrogated from the outside of the container, without requiring that the container be opened prior to interrogation. 1. A method for interrogating a sample for an analyte of interest , the method comprising:providing a container adapted to contain the sample, the container comprising a microstructured surface configured to provide capillary forces to retain a sample of interest;centrifuging the container toward the microstructured surface to form a sediment and a supernatant of the sample;inverting the container, after centrifuging the container, to decant at least a portion of the supernatant of the sample from the microstructured surface, such that a concentrate of the sample is retained in the microstructured surface, the concentrate comprising the sediment; andinterrogating the concentrate in the microstructured surface for the analyte of interest;wherein interrogating the concentrate in the microstructured surface includes interrogating for at least one of absorbance, transmittance, fluorescence, chemiluminescence, and a combination thereof.2. The method of claim 1 , wherein interrogating the concentrate in the microstructured surface includes optically interrogating concentrate in the microstructured surface.3. The method of ...

Подробнее
12-01-2017 дата публикации

METHODS OF DETERMINING A HIGH DENSITY LIPOPROTEIN PHOSPHOLIPID LEVEL IN A SAMPLE

Номер: US20170010290A1
Принадлежит:

The present disclosure provides economical and scalable (e.g., high-throughput) methods of determining an HDL-PL level in a sample from a subject, and methods of treating a subject comprising determining an HDL-PL level in a sample from the subject. 1. A method of determining a level of HDL-PL associated with a subject , the method comprising:removing non-HDL lipoproteins from a sample associated with the subject to produce a purified HDL-PL composition;contacting the purified HDL-PL composition with an indicator system; andmeasuring a concentration of the HDL-PL as a function of at least an absorbance of the indicator system.2. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises contacting the sample with a precipitation reagent comprising dextran claim 1 , a Mgsalt and sodium azide.3. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises contacting the sample with apolipoprotein B antisera to immunoprecipitate non-HDL particles.4. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises ultracentrifuging the sample and thereafter removing any fractions having a density of at least 1.063 g/mL.5. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises gel filtration of the sample.6. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises gel filtering the sample.7. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises filtering the sample through a column.8. The method of claim 1 , wherein the step of removing non-HDL lipoproteins comprises contacting the sample with cholesterol oxidase claim 1 , peroxidase claim 1 , phospholipidase D and N claim 1 ,N-bis-(4-sulfobutyl)-m-toulidine disodium.9. The method of claim 1 , wherein the indicator system comprises phospholipase D claim 1 , choline oxidase claim 1 , N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3 claim 1 ,5-dimethoxyaniline claim 1 , 4- ...

Подробнее
11-01-2018 дата публикации

AUTOMATED SYSTEM FOR PROCESSING PARTICLES

Номер: US20180010990A1
Принадлежит:

A method for processing particles contained in a liquid biological sample is presented. The method uses a rotatable vessel for processing particles contained in a liquid biological sample. The rotatable vessel has a longitudinal axis about which the vessel is rotatable, an upper portion comprising a top opening for receiving the liquid comprising the particles, a lower portion for holding the liquid while the rotatable vessel is resting, the lower portion comprising a bottom, and an intermediate portion located between the upper portion and the lower portion, the intermediate portion comprising a lateral collection chamber for holding the liquid while the rotatable vessel is rotating. The method employs dedicated acceleration and deceleration profiles for sedimentation and re-suspension of the particles of interest. 1. A method for separating particles from a liquid biological sample and re-suspending the particles in a secondary liquid , the method comprising: a longitudinal axis about which the vessel is rotatable,', 'an upper portion comprising a top opening for receiving the liquid comprising the particles,', 'a lower portion for holding the liquid while the rotatable vessel is resting, the lower portion comprising a bottom, and', 'an intermediate portion located between the upper portion and the lower portion, the intermediate portion comprising a lateral collection chamber for holding the liquid while the rotatable vessel is rotating;, 'a) introducing the liquid biological sample comprising the particles into a rotatable vessel through the top opening of the rotatable vessel such that the liquid is held by the lower portion of the rotatable vessel, wherein the rotatable vessel comprises'}b) rotating the rotatable vessel about its longitudinal axis at a rotational speed, wherein the liquid comprising the particles is moved to the lateral collection chamber by centrifugal force, and wherein the centrifugal force is sufficient to sediment the particles in the ...

Подробнее
10-01-2019 дата публикации

Rapid Sample Preparation for Analytical Analysis Using Dispersive Energized Extraction

Номер: US20190011337A1
Принадлежит: CEM CORPORATION

An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure. 124-. (canceled)25. An extraction based sample preparation method comprising:placing an extraction solvent, and a sample matrix that contains an analyte into a heat conductive sample cup surrounded by a pressure-resistant reaction chamber with the heat conductive sample cup having one opened filtered end and a mouth opposite from its open filtered end;adding liquid extraction solvent to both the inside of the sample cup and to the reaction chamber outside of the sample cup;sealing the sample cup between a sealing cover at the cup mouth and an outlet valve at the opposite opened filtered end of the reaction chamber so that the heating step increases the vapor pressure of the liquid extraction solvent above the solvent's vapor pressure at ambient temperature and pressure; andheating the liquid extraction solvent in the reaction chamber outside of the sample cup to in turn heat the sample cup, the sample matrix and the liquid extraction solvent until the temperature generates an above-atmospheric pressure that together with the increased temperature drives the analyte substantially from the sample matrix into the liquid extraction solvent;releasing the liquid solvent extract from the sample cup into a cooling tube at atmospheric pressure in which the cooling ...

Подробнее
10-01-2019 дата публикации

Instrument for analytical sample preparation

Номер: US20190011338A1
Принадлежит: CEM Corp

An instrument for extraction based molecular sample preparation and related processes is disclosed. The instrument includes a thermally conductive pressure resistant heating chamber and a thermally conductive sample cup positioned in the thermally conductive pressure resistant heating chamber for heating liquids and solids together in the thermally conductive sample cup. A liquid delivery inlet fixture in the thermally conductive pressure resistant heating chamber delivers liquids (solvent) from a supply to the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber, and a chiller in liquid communication with the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber receives heated liquids from the thermally conductive pressure resistant heating chamber when the chamber is opened to atmospheric pressure.

Подробнее
10-01-2019 дата публикации

Rapid Energized Dispersive Solid Phase Extraction (SPE) for Analytical Analysis

Номер: US20190011339A1
Принадлежит: CEM CORPORATION

An energized dispersive extraction method for sample preparation for analysis is disclosed. The method includes the steps of placing an extraction solvent, sorbent particles, and a sample matrix containing an analyte in a heat conductive sample cup; positioning the sample cup in a pressure-resistant reaction chamber; dispersing the solvent and the sample matrix in the sample cup in the reaction chamber; heating the sample matrix and the solvent in the sample cup in the reaction chamber to a temperature that generates an above-atmospheric pressure; draining the solvent extract from the sample cup at atmospheric pressure; and collecting the solvent extract for analysis. 126-. (canceled)27. A dispersive extraction based sample preparation method comprising:placing liquid extraction solvent, sorbent particles, and a sample matrix that contains an analyte into a heat conductive sample cup surrounded by a pressure-resistant reaction chamber with the heat conductive sample cup having one opened filtered end opening into the reaction chamber;adding liquid extraction solvent to both the inside of the sample cup and to the reaction chamber outside of the sample cup;heating the liquid extraction solvent in the reaction chamber outside of the sample cup to in turn heat the sample cup, the sample matrix, the sorbent particles and the liquid extraction solvent until the temperature generates an above-atmospheric pressure that together with the increased temperature drives the analyte substantially from the sample matrix into the liquid extraction solvent; anddraining the liquid solvent extract from the sample cup through the one open filtered end into a cooling tube at atmospheric pressure.28. A dispersive extraction based sample preparation method according to wherein:the draining step is carried out until the drained liquid extraction solvent approaches or reaches ambient temperature; andthereafter collecting the cooled release solvent for analysis.29. A dispersive extraction ...

Подробнее
10-01-2019 дата публикации

Instrument for Analytical Sample Preparation

Номер: US20190011340A1
Принадлежит: CEM CORPORATION

An instrument for extraction based molecular sample preparation and related processes is disclosed. The instrument includes a thermally conductive pressure resistant heating chamber and a thermally conductive sample cup positioned in the thermally conductive pressure resistant heating chamber for heating liquids and solids together in the thermally conductive sample cup. A liquid delivery inlet fixture in the thermally conductive pressure resistant heating chamber delivers liquids (solvent) from a supply to the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber, and a chiller in liquid communication with the thermally conductive sample cup in the thermally conductive pressure resistant heating chamber receives heated liquids from the thermally conductive pressure resistant heating chamber when the chamber is opened to atmospheric pressure. 1. A combination for solvent extraction comprising:a heated, pressure-sealed reaction chamber;a heat conductive sample cup in said reaction chamber, said reaction vessel including one open filtered end and a mouth opposite said open filtered end;an extraction sample in said sample cup; anda liquid extraction solvent inside said sample cup and outside said sample cup between the exterior of said sample cup and the interior of said reaction chamber.2. A combination according to wherein said reaction chamber is formed of a thermally conductive material.3. A combination according to wherein said opened filtered end is a foraminous floor and a filter media on foraminous floor.4. A combination according to wherein said filter media is selected from the group consisting of filter paper claim 3 , glass fibers claim 3 , quartz fibers claim 3 , nonwoven polymers and combinations thereof.5. A combination according to wherein said extraction sample is selected from the group consisting of soil claim 1 , plastics claim 1 , prepared foods claim 1 , food packaging claim 1 , agricultural products claim ...

Подробнее
10-01-2019 дата публикации

Rapid Sample Preparation for Analytical Analysis Using Dispersive Energized Extraction

Номер: US20190011341A1
Принадлежит: CEM CORPORATION

An extraction method for preparing samples for analytical analysis is disclosed. The method includes the steps of placing a sample matrix containing one or more analytes in a heat conductive sample cup, positioning the heat conductive sample cup in a pressure-resistant reaction chamber, dispensing solvent into the heat conductive sample cup, dispersing the solvent and the sample matrix in the sample cup in the reaction chamber, heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure, and releasing the extraction solvent extract from the sample cup at atmospheric pressure. 1. An extraction method for preparing samples for analytical analysis comprising the steps of:placing a sample matrix containing one or more analytes in a heat conductive sample cup;positioning the heat conductive sample cup in a pressure-resistant reaction chamber;dispensing solvent into the heat conductive sample cup;heating the sample matrix and the extraction solvent in the heat conductive sample cup in the reaction chamber to a temperature at which the dispensed solvent generates an above-atmospheric pressure; andreleasing the extraction solvent extract from the sample cup at atmospheric pressure.2. An extraction method according to further comprising:releasing the solvent into a cooling coil that has a length sufficient to reduce the temperature of the solvent extract to ambient or near-ambient temperature in the coil; andcollecting the filtered solvent extract from the coil.3. An extraction method according to wherein the sample cup has an open mouth at one end and a partially open floor at the opposite end claim 1 , and the step of dispensing solvent into the sample cup is carried out from positions selected from the group consisting of the top of the sample cup claim 1 , the bottom of the sample cup claim 1 , and top and bottom of the sample cup.4. ...

Подробнее
10-01-2019 дата публикации

PARTICLE SIZE PURIFICATION METHOD AND DEVICES

Номер: US20190011342A1
Принадлежит: University of Notre Dame

A panicle separation multi-membrane matrix device and method are provided. The particles isolated may comprise noun-scale particles, such extracellular membrane vesicles, having a size of about 50 to about 150 nm. The vesicles are released by many different cell types, and may be efficiently isolated at high yield and purity according to the present methods from various body fluids (e.g., blood, saliva, breast milk, serum, plasma, ascites fluid, etc.). Such isolated exosome preparations may include biomarkers, such as disease biomarkers (diagnostic markers) for various disease (early stage and late stage cancers, neurological disorders (Parkinson disease, Alzheimer disease), diabetes, pancreatic diseases, renal failure, infectious diseases (HIV, tuberculosis, malaria, hepatitis)). The present methods and devices may be used to detect and monitor animals (human, live-stock, companion animal) for infectious diseases, such as tuberculosis and other diseases. The methods and devices require minimal sample material (10 μl), are rapid, economical, yield highly enriched small molecule (e.g., exosoines) preparations, and do not require electricity. 2. The particle size separation device of wherein the series of membrane filters of decreasing size comprise an 80 μm 10 μm and 0.2 μm filter membrane.3. The particle size separation device of wherein a washer is included between each membrane filter in the series of membrane filters.4. The particle size separation device of claim lwhrein the resin matrix comprises5. The particle size separation device of further defined as a separation column.6. The particle size separation devise of wherein the device removes particles having a size of greater than about 150 nm to about 200 nm from a sample.7. The particle size separation devise of wherein the sample is a body fluid.8. The particle size separation device of wherein the body fluid is blood claim 7 , saliva claim 7 , breast milk claim 7 , serum claim 7 , plasma claim 7 , or ...

Подробнее
14-01-2021 дата публикации

A METHOD OF DETECTING AND DIAGNOSING THE PROGRESSION OF DIABETES

Номер: US20210010939A1
Принадлежит:

The subject of the invention is the method of detecting and diagnosing the progression of diabetes using Raman spectroscopy which involves the examination of the changes in the composition of urinary extracellular vesicles which confirm the existence of the condition and its progression. The invention can be applied in clinical practice, in particular in the early clinical diagnostics of diabetes and in the monitoring of its progression, in particular diabetic nephropathy and advanced renal impairment caused by diabetes. 1. The method of detecting and diagnosing diabetes wherein the change in the composition of microvesicles in a tissue or liquid sample collected from a patient is examined , and this change confirms the presence of diabetes and its progression.2. The method according to wherein the change in the composition of urinary extracellular vesicles (UEV) is examined.3. The method according to wherein there are the following stages:a) Urinary extracellular vesicles (UEV) are isolated from the urine sample,b) Raman spectra are registered and the analysis of the distribution of the intensity of characteristic bands is performed,c) If it is confirmed that the value of RI for the intensity of the Raman spectrum is lower than the value of RI for the Raman spectrum obtained in an identical way for the sample collected from a healthy individual, the patient is diagnosed with diabetes.4. The method according to wherein the urine sample is subject to centrifugation at 2000×g for about 30 minutes in stage a).5. The method according to wherein claim 3 , in stage a) claim 3 , the urine sample is concentrated using a dialysis membrane with large-diameter pores permeable for the molecules of the average molecular weight below 1000 Da (MWCO) claim 3 , which is followed by washing.6. The method according to wherein the washing solution contains silver chloride and sodium dichloroisocyanurate in the amount of 1 mg of silver chloride and 4.5 mg of sodium dichloroisocyanurate ...

Подробнее
15-01-2015 дата публикации

MICRO-ANALYZER WITH PASSIVE AGGREGATOR

Номер: US20150013439A1
Принадлежит:

A micro-analyzer is described. This micro-analyzer includes an outer surface region on a sampler surface that receives liquid droplets, and aggregates and moves the droplets radially toward an inner surface region on the sampler surface that receives the droplets. For example, the outer surface region may include a set of micro-patterned concentric rings, each of which includes a set of radially oriented wall-groove pairs. Moreover, the sampler surface may be increasingly less hydrophobic along a radial direction toward the center of the sampler surface, thereby creating an axisymmetric wettability gradient. After the droplets are aggregated, an analysis mechanism in an area within the inner surface region performs analysis on the aggregated droplets. 1. A micro-analyzer configured for on-chip micro-analysis , comprising:a substrate; an outer surface region configured to collect and transport liquid droplets radially toward a center of the patterned surface; and', 'an inner surface region within the outer surface region, wherein the inner surface region is configured to receive the liquid droplets collected by the outer surface region; and, 'a patterned surface disposed on the substrate, which further comprisesan analysis mechanism in an area in the inner surface region configured to analyze the collected droplets.2. The micro-analyzer of claim 1 , wherein the patterned surface is configured so that the surface is increasingly less hydrophobic along a radial direction toward the center of the patterned surface.3. The micro-analyzer of claim 1 , wherein the outer surface region is micro-patterned to create a wettability gradient which is distributed axisymmetrically.4. The micro-analyzer of claim 3 , wherein the wettability gradient increases in an inward radial direction.5. The micro-analyzer of claim 1 , wherein the outer surface region comprises a set of micro-patterned concentric rings.6. The micro-analyzer of claim 5 , wherein a given micro-patterned concentric ...

Подробнее
09-01-2020 дата публикации

Electromagnetic Assemblies for Processing Fluids

Номер: US20200011773A1
Принадлежит:

Methods and apparatus for processing fluids are described. In various aspects, a fluid processing system may include a magnetic assembly that includes a plurality of magnetic structures configured to generate a magnetic field gradient within a fluid container. The magnetic structures may be formed as a plurality of electromagnets configured to be individually actuated by a controller. Each of the electromagnets may generate a magnetic field within the fluid container. The electromagnets may be differentially actuated to create a magnetic field gradient within the fluid container to agitate, mix, or otherwise influence magnetic particles disposed within the fluid container. Activation of the electromagnets of an electromagnetic structure may generate a magnetic field gradient that influences magnetic particles in an x-y direction. In addition, activation of the electromagnets of a plurality of electromagnetic structures may generate magnetic field gradients that influences magnetic particles in an x-y direction and z-direction. 1. A fluid processing system , comprising: wherein the magnetic structure is configured to receive a fluid container defining a fluid chamber therein for containing a fluid and a plurality of magnetic particles, and', "wherein each of the plurality of electromagnets are configured to generate a magnetic field within the fluid container disposed on the center axis of the magnetic structure when an electrical signal is applied to each of the electromagnets' electrically-conductive coil; and"], 'a magnetic assembly comprising at least one magnetic structure, each magnetic structure comprising a plurality of electromagnets disposed about a center axis, wherein each of the plurality of electromagnets has an electrically-conductive coil disposed about a centerline extending toward the center axis of the magnetic structure,'}a control component coupled to the at least one magnetic structure, the control component being configured to control the ...

Подробнее
09-01-2020 дата публикации

SIZE-BASED SEPARATION OF DISSOCIATED FIXED TISSUES

Номер: US20200011775A1
Принадлежит:

The present disclosure provides a method of separating cellular particles from a tissue sample and then sorting the cellular particles into two or more cellular particle populations. 1. A method of segregating cellular particles from a tissue sample comprising: (i) homogenizing the tissue sample to provide a homogenized sample; and (ii) sorting cellular particles in the homogenized tissue sample by size into at least a first cellular particle population and a second cellular particle population.2. The method of claim 1 , wherein the cellular particles include cells.3. The method of claim 1 , wherein the cellular particles include cell nuclei.4. The method of claim 1 , wherein the second cellular particle population comprises cellular particles derived from tumor cells.5. The method of claim 4 , wherein the cellular particles derived from tumor cells have an average diameter ranging from between about 12 μm to about 50 μm or from between about 8.5 μm to about 30 μm.6. The method of claim 5 , wherein the tumor cells are derived from at least one of a whole tumor claim 5 , a partial tumor claim 5 , a metastatic tumor claim 5 , a partial metastatic tumor claim 5 , or lymph nodes.7. The method of claim 1 , wherein the tissue sample is derived from at least one of residual surgical material or a biopsy sample.8. The method of claim 1 , wherein the tissue sample is one that was fixed in a crosslinking solution.9. The method of claim 1 , wherein the tissue sample is derived from a sample embedded in paraffin.10. The method of claim 1 , wherein the sorting of the cellular particles in the homogenized sampled by size does not require the step of staining the cellular particles.11. The method of claim 1 , wherein the cellular particles within the homogenized tissue sample are sorted with a microfluidic device.12. A method of segregating cells from a tissue sample comprising:homogenizing a tissue sample to provide a homogenized tissue sample;sorting cells in the homogenized ...

Подробнее
03-02-2022 дата публикации

System for imaging andmonitoring fluids

Номер: US20220032299A1
Автор: Dan Zemer, Oz Bornstein
Принадлежит: VBACT Ltd

The present disclosure concerns a system for imaging and monitoring fluids to identify the presence of objects therein. Objects that are being identified by the system include, for example, microorganisms, particles, bacteria, cells, foreign substances (e.g. bubbles of air in a liquid, substance that may change visual parameters of the main liquid such as color and transparency), etc. Identification of the objects by the system is then may be followed by analysis to conclude the status of the system (e.g. identifying contamination, impurities, etc.)

Подробнее
19-01-2017 дата публикации

APPARATUS, SYSTEM, AND METHOD FOR COLLECTING A TARGET MATERIAL

Номер: US20170014819A1
Принадлежит: RareCyte, Inc.

This disclosure is directed to an apparatus, system and method for retrieving target material from a suspension. A system includes a processing vessel, a displacement fluid, and a tube. The tube includes a funnel, a cannula, and a cavity. The cannula allows for fluid communication between the funnel and the cavity, such that the processing vessel is inserted into the cavity, and the cannula extends into the processing vessel. 1. A system comprising: a first end,', 'a second end comprising an aperture,', 'a biological suspension comprising a target material, and', a funnel comprising a mouth proximal to the first end and an apex distal to the first end,', 'a cavity extending from the aperture in the second end towards the apex of the funnel, and', 'a cannula extending from the apex of the funnel into the cavity; and, 'a collection segment between the first and second ends, the collection segment comprising'}], 'a tube comprising'}a processing vessel comprising a closed end and a displacement fluid having a density greater than a density of the target material,wherein at least a portion of the processing vessel is located within the cavity of the tube, andwherein the cannula extends through the closed end of the processing vessel.2. The system of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone claim 1 , a polysaccharide solution claim 1 , iodixanol claim 1 , an organic solvent claim 1 , a liquid wax claim 1 , an oil claim 1 , a gas claim 1 , olive oil claim 1 , mineral oil claim 1 , silicone oil claim 1 , immersion oil claim 1 , mineral oil claim 1 , paraffin oil claim 1 , silicon oil claim 1 , fluorosilicone claim 1 , perfluorodecalin claim 1 , perfluoroperhydrophenanthrene claim 1 , perfluorooctylbromide claim 1 , organic solvents claim 1 , 1 claim 1 ,4-Dioxane claim 1 , acetonitrile claim 1 , ethyl acetate claim 1 , tert-butanol claim 1 , cyclohexanone claim ...

Подробнее
03-02-2022 дата публикации

DEVICE FOR CAPTURING IN SITU AQUATIC MICROBIOMES

Номер: US20220033872A1
Принадлежит:

The present disclosure relates to a portable device for collecting and/or concentrating in situ plankton microbiome, configured for submersion in water. The device herein disclosed is a compact and low-cost autonomous biosampler, with the ability to yield DNA samples for later genomic analysis. 1. A portable device for collecting and/or concentrating in situ plankton microbiome configured for submersion in water , comprising:an inlet for water containing the plankton microbiome;an outlet for water depleted of plankton microbiome;a plurality of valves placed between the inlet and the outlet;a set of sensors for measuring flow and pressure;a pump for pumping water from the inlet to the outlet such that water is passed across a filter cartridge,wherein the filter cartridge comprises a plurality of filters for in situ filtration of water containing plankton microbiome;a microcontroller for controlling the opening and closing of a plurality of valves and the speed of water pumping such that the device collects and/or concentrates in situ plankton microbiome;a reservoir containing a preserving solution for preserving nucleic acids.2. The device according to claim 1 , wherein the filter cartridge comprises at least 16 filters each having a pore size of 0.22 μm.3. The device according to claim 1 , comprising at least 2 filter cartridges.4. The device according to claim 1 , wherein the preserving solution is injectable into the filter cartridge.5. The device according to claim 1 , wherein the set of sensors comprises a pressure sensor for controlling and maintaining the pressure of the filter cartridge from 1 bar to 1.3 bar.6. The device according to claim 1 , wherein the set of sensors comprises a flow sensor for detecting and/or controlling the flow of water passing through the filter cartridge.7. The device according to claim 1 , for concentrating in situ plankton microbiome nucleic acids by filtration.8. The device according to claim 1 , wherein said device operates at a ...

Подробнее
03-02-2022 дата публикации

SAMPLE PREPARATION FOR DIFFICULT SAMPLE TYPES

Номер: US20220034770A1
Принадлежит:

Devices and methods are provided for collecting and handling difficult sample types. 1. A method of amplifying RNA from a direct blood sample comprising:(a) adding the direct blood sample to a sample buffer,(b) bead beating the sample buffer,(c) extracting the RNA from the sample buffer, and(d) amplifying the RNA.2. The method of claim 1 , wherein steps (b) through (d) are performed in a closed sample vessel.3. The method of claim 1 , wherein steps (a) through (d) are performed without centrifugation claim 1 , ethanol precipitation claim 1 , and DNA digestion.4. The method of claim 1 , wherein the RNA is host RNA.5. The method of claim 1 , wherein the direct blood sample is collected in a RNA stabilized solution.6. The method of claim 1 , wherein the direct blood sample is collected in a non-RNA stabilizing solution.7. The method of claim 1 , wherein the direct blood sample is separated prior to step (a) and a blood fraction is used.8. The method of claim 1 , further comprising filtering the sample between steps (a) and (b).9. The method of claim 1 , wherein the RNA is provided in high copy number.10. The method of claim 1 , wherein the RNA is from microorganisms.11. The method of claim 1 , further comprising washing the RNA following the extracting step claim 1 , wherein the washing step comprises no more than three washes.12. The method of claim 1 , wherein the sample buffer includes a guanidinium compound.13. The method of claim 1 , wherein step (g) includes a first-stage multiplex PCR and a plurality of individual second-stage PCR reactions. This application is a divisional application of U.S. patent application Ser. No. 15/340,612, filed Nov. 1, 2016, which claims the benefit, under 35 U.S.C. § 119(e), of U.S. Provisional Application Ser. No. 62/249,592, filed Nov. 2, 2015, the entire contents of each of which are incorporated by reference herein.Embodiments of the present disclosure relate generally to methods and devices for extracting nucleic acids from a ...

Подробнее
03-02-2022 дата публикации

System and Method of Automatically Preparing and Analyzing Urine Samples for Identifying Cancer Cells

Номер: US20220034919A1
Автор: Zarate Alfredo R.
Принадлежит:

A system and method of automatically preparing and analyzing urine samples for identifying cancer cells is able to complete conventional diagnostic tasks without lab technicians, cytopathologists, or other medical professionals. The method is provided with at least one source sample, at least one manipulator arm, at least one centrifuge, at least one electronic microscope, and at least one unitary controller. The method is further provided with a cytopathological index containing a visual characteristic database and identification confidence threshold rubrics supporting the automation of visual analyses typically performed manually with a conventional microscope. This method is further provided with a data processing function, wherein data stemming from multiple testing cycles may be collated, formatted, and presented for use by medical professionals in determining and projecting the effectiveness of a course of treatment. 1. A method of automatically preparing and analyzing urine samples for identifying cancer cells , the method comprises the steps of:(A) providing at least one source sample, at least one manipulator arm, at least one centrifuge, at least one electronic microscope, and at least one unitary controller, wherein the unitary controller is communicably coupled to the manipulator arm, the centrifuge, and the electronic microscope, wherein a cytopathological index is stored on the unitary controller;(B) preparing the source sample into a plurality of sample tubes with the manipulator arm, wherein each sample tube includes a sample identification;(C) loading the sample tubes into the centrifuge with the manipulator arm;(D) executing a separation process on the sample tubes with the centrifuge;(E) removing the sample tubes from the centrifuge with the manipulator arm;(F) extracting a plurality of sediment samples with the manipulator arm, wherein each sample tube is associated to a corresponding sediment sample from the plurality of sediment samples;(G) ...

Подробнее
21-01-2016 дата публикации

Quick extraction kit adapted to a procedure of detecting pesticide residues in agricultural products and a method of obtaining a primary test liquid from an agricultural sample by the quick extraction kit

Номер: US20160018305A1
Принадлежит:

The present invention provides a quick extraction kit adapted to a procedure of detecting pesticide residues in agricultural products and a method of obtaining a primary test liquid from an agricultural sample by the quick extraction kit. The quick extraction kit comprises a pipe, a first powder mixture layer and a second powder mixture layer. The method of taking primary test liquid is performed as follows. First, obtaining fragments of the agricultural sample. Second, adding an extraction solvent into the fragments of the agricultural sample to obtain a sample solution. Third, adding the sample solution into the pipe. Finally, driving the sample solution to export from the pipe to become the primary test liquid. The quick extraction kit and the method solve the problem of being unable to quickly obtain the result of detecting pesticide residues. 1. A quick extraction kit for a procedure of detecting pesticide residues in agricultural products , comprising:a pipe, having an output port located at the bottom of the pipe and an input port located at the top of the pipe, wherein the output port is adapted to be input a sample solution, and the sample solution is a mixture solution obtained by a treatment of shaking homogenized fragments of an agricultural sample with an extraction solvent;a first powder mixture layer, being a form of powder and filled in the pipe, the first powder mixture layer capable of absorbing most of water of the sample solution and buffering a pH value of the sample solution when the sample solution flows through the first powder mixture layer; anda second powder mixture layer, being a form of powder, filled in the pipe and between the first powder mixture layer and the output port, the second powder mixture layer capable of absorbing the rest of water of the sample solution and impurities interfering with a detection of the sample solution.2. The quick extraction kit of claim 1 , wherein a density of the first powder mixture layer in the pipe ...

Подробнее
21-01-2021 дата публикации

REAGENT CARTRIDGE

Номер: US20210016286A1
Автор: Koch Dale, SWANSON Todd
Принадлежит:

In one embodiment, a cartridge includes at least one compartment and a reagent in the at least one compartment. The reagent is a chemical composition for testing at least one of soil and vegetation for a chemical contained in the soil or vegetation. The reagent can be used in a soil and/or vegetation analysis test. The cartridge can contain an authentication chip to ensure that the reagent is the correct reagent for the analysis test. 1. A cartridge comprising:at least one compartment;a reagent in the at least one compartment, wherein the reagent is a chemical composition for testing at least one of soil and vegetation for a chemical contained in the soil or vegetation; andwherein the cartridge is adapted to cooperate with a soil and/or vegetation analysis system to supply the reagent to the soil and/or vegetation analysis system.2. The cartridge of claim 1 , further comprising an authentication device.3. The cartridge of claim 2 , wherein the authentication device comprises a chip adapted to be connected to a network claim 2 , wherein when connected to the network claim 2 , the authentication chip is accessed by the network to confirm that the cartridge is an authorized cartridge containing the reagent that is specific for a soil and/or vegetation test.4. The cartridge of further comprising a meter to measure an amount of reagent dispensed from the cartridge claim 1 , wherein the meter communicates the amount of reagent dispensed from the cartridge to a network.5. (canceled)6. The cartridge of further comprising a counter to count a number of times reagent is dispensed from the cartridge to determine consumed volume of reagent claim 1 , wherein the counter communicates with a network to communicate the number of times.7. The cartridge of further comprising a time counter to count time for determining an amount of reagent dispensed from the cartridge claim 1 , wherein the time counter communicates with a network to communicate the amount of time.8. The cartridge of ...

Подробнее
17-01-2019 дата публикации

MITOCHONDRIA EXTRACTION APPARATUS

Номер: US20190017909A1
Принадлежит:

A mitochondria extraction apparatus provided in the present invention includes a container used for holding a mixture fluid and a diafiltration assembly disposed in the container and used for isolating mitochondria. A basement of the mitochondria extraction apparatus is disposed in the container. During a circular motion of the mitochondria extraction apparatus, a centrifugal force in the mitochondria extraction apparatus acts on the mixture fluid in a hole-shaped microfluidic channel included in the diafiltration assembly, so as to provide a pushing force for the mixture fluid, enabling the mixture fluid to rapidly flow in the microfluidic channel for diafiltration. 1. A mitochondria extraction apparatus , making a circular motion with a moving axis being the center under the effect of an external force , comprising:a container, having a first receiving chamber used for receiving an external mitochondria-containing mixture fluid to be separated, and a second receiving chamber and the first receiving chamber being separated from each other;a diafiltration assembly, having a basement, located between the first receiving chamber and the second receiving chamber, at least one hole-shaped microfluidic channel being disposed in the basement in a manner of extending in a direction perpendicular to the moving axis, a flow inlet hole opening at one end being connected to the first receiving chamber, a flow outlet hole opening at the other end being connected to the second receiving chamber, and the flow inlet hole opening being located between the flow outlet hole opening and the moving axis, whereinafter the mitochondria-containing mixture fluid to be separated enters the microfluidic channel from the first receiving chamber via the flow inlet hole opening and diafiltration is performed, mitochondria flow out from the flow outlet hole opening and flow into the second receiving chamber, and when the mitochondria extraction apparatus makes a circular motion, the mitochondria ...

Подробнее
21-01-2021 дата публикации

MICROWAVE EXTRACTION SYSTEM

Номер: US20210017469A1
Автор: Hsieh Chen
Принадлежит:

A microwave extraction system comprises a sealed microwave box, a stirrer, a vacuum extraction tank, a vacuum machine, and an ice-water circulation unit; wherein the sealed microwave box is a tank-body set with microwave generators for accommodating the vacuum extraction tank and the stirrer, and the microwave generated by the microwave generators can penetrate into the tank-body; wherein the tank-body is set with a sealing cap to form a sealed space, and the stirrer can stir the object to be extracted; wherein the vacuum extraction tank is for placing the object to be extracted and providing the microwave penetration; wherein the ice-water circulation unit provides ice water to the tank-body; after completing the extraction operation, the ice-water circulation unit is turned-off and microwave-control to an appropriate temperature by the microwave generators; then turning-on the vacuum to perform concentration under reduced pressure; thereby improving microwave efficiency and reducing maintenance cost. 1wherein the sealed microwave box is a tank-body for accommodating the vacuum extraction tank and the stirrer;wherein at least two sets of notches are recessedly set on the side walls of the tank-body, and the side walls of the notches are set with waterproof wave-transmittable plates;wherein at least two sets of notches are recessedly set on the side walls of the tank-body, and the side walls of the notches are set with waterproof wave-transmittable plates;wherein the two sets of notches are set with microwave generators, which the microwave generated by the microwave generators can penetrate into the tank-body through the waterproof wave-transmittable plates;wherein the notches are set with protective plates facing the outer sides of the microwave generators, and the protective plates can prevent and block the microwave generated by the microwave generators from leaking out;wherein the tank-body is set with a sealing cap which is pivotally set with sealing drivers, ...

Подробнее
22-01-2015 дата публикации

Method and apparatus for sorting fibers

Номер: US20150021238A1

An apparatus and methods for sorting and determining the length distribution of fibers in a sample are disclosed. One method of sorting and determining the length distribution of fibers includes immersing the fibers in a liquid to form a mixture; placing the mixture into a stack of sieves; progressively applying a predetermined number of frequencies of sound energy to each sieve in the stack of sieves; draining the mixture from the stack of sieves; and quantifying the collected fibers in each sieve in the stack of sieves.

Подробнее
21-01-2021 дата публикации

Devices and Methods for Processing Fluid Samples

Номер: US20210018465A1
Принадлежит: DH TECHNOLOGIES DEVELOPMENT PTE LTD

Provided is the processing of sample fluids containing one or more analytes of interest and to methods and devices for separating and/or purifying components of a sample fluid using electric and hydrodynamic forces. Though the fluid processing systems and methods are generally described herein as applied to microfluidics, it will be appreciated that the fluid processing systems may process any fluid volume suitable for use in embodiments described herein. Y-shaped and multiple-branched shaped 2-D EFD devices have been used to separate and/or purify one or more analytes from a mixture. Systems and methods in accordance with various aspects of the present teachings utilize hydrodynamic pressure (e.g., using a pump) to drive the sample liquid from the sample inlet to the separation stream, and can, in some aspects, provide improved control of the movement of the analytes, improved processing times, and decreased buffer depletion.

Подробнее
28-01-2016 дата публикации

Automated Sample Processing, Fluid Distribution, and Sedimentation Assay

Номер: US20160023204A1
Принадлежит:

The disclosure describes methods and devices with which to process and analyze difficult chemical, biological, environmental samples including but not limited to those containing bulk solids or particulates. The disclosure includes a cartridge which contains a separation tube as well as one or more valves and cavities for receiving raw sample materials and for directing and containing various fluids or samples. The cartridge may contain a separation fluid or density medium of defined density, and structures which direct particulates toward defined regions of the cartridge. Embodiments can include a rotational device for rotating the cartridge at defined rotational rates for defined time intervals. Embodiments allowing multiple assays from a single sample are also disclosed. In some embodiments, this device is used for direct processing and chemical analysis of food, soil, blood, stool, motor oil, semen, and other samples of interest. 1. An apparatus comprising: a receiving cavity configured to hold the sample, the receiving cavity having a sample entry hole configured to allow entry of the sample into the receiving cavity;', 'a second cavity located farther from the center of rotation of the cartridge relative to a location of the receiving cavity; and', 'a passage connecting the receiving cavity and the second cavity, the passage having a cross-sectional area that is smaller than a cross-sectional area of the receiving cavity,', 'wherein the cartridge is configured to transfer the sample from the receiving cavity to the second cavity during rotation of the cartridge for sedimentation of the particles or cells., 'a cartridge having a center of rotation and configured to be rotated to cause sedimentation of particles or cells suspended in a sample, the cartridge comprising2. The apparatus of wherein the cartridge is configured for conducting a sandwich assay method comprising:generating complexes on a plurality of the particles in the sample, wherein a complex ...

Подробнее
17-04-2014 дата публикации

Microfluidic system for high-throughput, droplet-based single molecule analysis with low reagent consumption

Номер: US20140106462A1
Принадлежит: JOHNS HOPKINS UNIVERSITY

A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region.

Подробнее
22-01-2015 дата публикации

SYSTEM, APPARATUS AND METHOD FOR MATERIAL PREPARATION AND/OR HANDLING

Номер: US20150024480A1
Принадлежит:

Oscillating angularly rotating a container containing a material may cause the material to be separate. Denser or heavier material may unexpectedly tend to collected relatively close to the axis of rotation, while less dense or light material may tend to collect relatively away from the axis of rotation. Oscillation along an arcuate path provides high lysing efficiency. Alternatively, a micromotor may drive an impeller removably received in a container. Lysing may be implemented in batch mode, flow-through stop or semi-batch mode, or flow-through continuous mode. Lysing particulate material may exceed material to be lysed or lysed material and/or air may be essentially eliminated from a chamber to increase lysing efficiency. 1110-. (canceled)111. An article to perform flow-through lysis by oscillation on material to be lysed , the article comprising:at least one wall forming at least one chamber having a first opening and at least a second opening spaced from the first opening, the first and the second openings to provide fluid communication into the chamber from an exterior thereof;a particulate lysing material received in the chamber, the particulate material including a plurality of particles sized to lyse a material to be lysed and having a combined particulate volume;a first filter received in the chamber between the first opening and the particulate material, the first filter having a plurality of apertures sized to substantially pass the material to be lysed and to retain the particulate material;a second filter received in the chamber between the second opening and the particulate material, the second filter having a plurality of apertures sized to pass the material to be lysed and to retain the particulate material, wherein the first filter and the second filter form a particulate retainment area therebetween having a volume sufficiently greater than said combined particulate volume to permit the plurality of particles to move relative to one another to ...

Подробнее
25-01-2018 дата публикации

HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS USING MAGNETIC BEADS FOR HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS

Номер: US20180023112A1
Принадлежит:

A method of transferring material from a first microfabricated device to a second microfabricated device. At least one magnetic bead is loaded into at least one microwell of the first microfabricated device, where a plurality of cells are cultivated. The second microfabricated device is positioned such that the at least one microwell of the first array of microwells is aligned with at least one microwell of the second array of microwells. A magnetic field is applied so as to move the at least one magnetic bead contained in the at least one microwell of the first microfabricated device into the at least one microwell of the second microfabricated device. In this manner, at least one cell from the plurality of cells in the at least one microwell of the first microfabricated device is transferred to the at least one microwell of the second microfabricated device. 1. A method of transferring material from a first microfabricated device including a first array of microwells to a second microfabricated device including a second array of microwells , the method comprising:loading at least one magnetic bead into at least one microwell of the first array of microwells;incubating the first microfabricated device to cultivate a plurality of cells in at least one microwell of the first array of microwells;positioning the second microfabricated device relative to the first microfabricated device such that the at least one microwell of the first array of microwells of the first microfabricated device is aligned with at least one microwell of the second array of microwells of the second microfabricated device; andapplying a magnetic field so as to move the at least one magnetic bead contained in the at least one microwell of the first array of microwells into the at least one microwell of the second array of microwells, whereby at least one cell from the plurality of cells in the at least one microwell of the first array of microwells is transferred to the at least one microwell ...

Подробнее
28-01-2016 дата публикации

METHOD AND APPARATUS FOR THE EXTRACTION OF VITAMIN D METABOLITES FROM HUMAN PLASMA

Номер: US20160025606A1
Принадлежит:

This invention relates to a method and apparatus or kit for extracting major metabolites of vitamin D from human plasma or serum. More particularly, the invention provides for the extraction from human plasma or serum samples comprising vitamin D metabolites such as 1,25-dihydroxy vitamin D3, 25-hydroxy vitamin D2, and D3 from protein binding, removal of protein and phospholipids, and isolation of the metabolites using a combination of ion-exchange and Lewis acid mechanisms without the requirement to acidify the samples. The method and apparatus of the invention comprise a cartridge or plurality of cartridges comprising at least one protein crash frit, a strong cation exchanged sorbent, and an acidified alumina sorbent to provide higher recoveries of vitamin D metabolites than existing phospholipid depletion plate techniques. Accurately quantifying 1,25-dihydroxy vitamin D3 is useful in differential diagnosis of vitamin D-related diseases and for monitoring vitamin D therapy in patients with chronic renal disease. 1. An apparatus for isolating metabolites of vitamin D or small molecules from a non-acidized serum or plasma sample comprising proteins , metabolites of vitamin D , small molecules , phospholipids and interfering compounds in an aqueous media , said apparatus comprising:at least one well having a generally tubular side wall defining an interior well space having an upper inlet and a lower outlet;a first protein crash frit disposed in the interior well space between the upper inlet and the lower outlet defining an introduction zone above the first protein crash frit and between the upper inlet and the first protein crash frit;a first sorbent zone comprising a cation exchanged silica sorbent disposed adjacent to and below the first protein crash frit;a second protein crash frit disposed in the interior well space between the first sorbent zone and the second sorbent zonea second sorbent zone disposed below said first adsorbent zone comprising an acidized ...

Подробнее
28-01-2016 дата публикации

SAMPLE CONCENTRATOR AND METHOD OF USE

Номер: US20160025607A1
Принадлежит:

The present disclosure provides an assembly for preparing a sample for analysis. The assembly comprises a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; a filter element operatively interposed in the fluid pathway; and a closure comprising a third opening and a chamber, the closure being slideably engaged with the outlet. The chamber and the outlet are dimensioned so that the outlet is sealingly engaged with the chamber. A method of using the assembly to detect a microorganism is also provided. 1. A method , comprising: a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; and', 'a filter element operatively interposed in the fluid pathway;, 'depositing a liquid sample suspected of containing a microorganism into a sample preparation device, the sample preparation device comprisingpassing the liquid sample through the filter element to retain the microorganism; 'wherein urging the outlet into the chamber comprises urging the outlet into the chamber to an extent sufficient to cause the back-flush liquid to contact the filter element; and', 'urging the outlet into a chamber configured to sealingly receive the outlet, the chamber containing a back-flush liquid;'}analyzing a portion of the back-flush liquid to detect a presence or an absence of the microorganism.2. The method of claim 1 , wherein urging the outlet into the chamber comprises urging the outlet into the chamber to an extent sufficient to cause a portion of the back-flush liquid to pass through the filter element.3. The method of claim 2 , wherein analyzing a portion of the back-flush liquid to detect a presence or an absence of the microorganism comprises analyzing a quantity of the portion of the back-flush liquid.4. The method of claim 1 , further comprising passing the liquid sample through a prefilter5. The method of claim 4 , wherein passing the liquid sample through a ...

Подробнее
28-01-2016 дата публикации

PARTICLE CONCENTRATION SYSTEM

Номер: US20160025677A1
Принадлежит: UNIVERSITY OF WASHINGTON

Methods and systems are provided for concentrating particles (e.g., bacteria, viruses, cells, and nucleic acids) suspended in a liquid. Electric-field-induced forces urge the particles towards a first electrode immersed in the liquid. When the particles are in close proximity to (e.g., in contact with) the first electrode, the electrode is withdrawn from the liquid and capillary forces formed between the withdrawing electrode and the surface of the liquid immobilize the particles on the electrode. Upon withdrawal of the electrode from the liquid, the portion of the electrode previously immersed in the liquid has particles immobilized on its surface. 1. A particle concentrating system , comprising:(a) a first electrode having a high aspect ratio, wherein the first electrode comprises a shaft having a shaft latitudinal dimension and a distal end having a distal latitudinal dimension, wherein the distal latitudinal dimension is from one nanometer to one millimeter;(b) an actuator configured to immerse and withdraw the first electrode from the first liquid such that a capillary force formed between the withdrawing first electrode and the first liquid immobilizes the first particle on a surface of the first electrode; and(c) an electric signal generator configured to generate an electrically induced force through the first electrode such that when the first electrode is immersed in a first liquid, a first particle in the first liquid is preferentially urged toward the first electrode.2. The system of claim 1 , further comprising the first liquid comprising the first particle.3. The system of claim 1 , wherein the shaft comprises a material selected from the group consisting of a metal claim 1 , a doped semiconductor claim 1 , and a conductive polymer.4. The system of claim 1 , wherein the shaft comprises a material selected from the group consisting of carbide nanowires claim 1 , carbon nanotubes claim 1 , and combinations thereof.5. The system of claim 1 , wherein the ...

Подробнее
26-01-2017 дата публикации

SYSTEMS, DEVICES, AND METHODS FOR BODILY FLUID SAMPLE COLLECTION, TRANSPORT, AND HANDLING

Номер: US20170023546A1
Принадлежит:

Bodily fluid sample collection systems, devices, and method are provided. The sample is collected at a first location and subjected to a first sample processing step. The sample may be shipped to a second location and subjected to a second sample processing step that does not introduce contaminants into a plasma portion of the sample formed from the first processing step. 1. A method for use with a bodily fluid sample from a subject , the method comprising:shipping a plurality of sample containers from a first location to a second location, wherein each of said sample containers contains about 500 μL or less but greater than about 30 μL of the bodily fluid sample, wherein interior volume of each of the sample containers is about 600 μL or less,subjecting the bodily fluid sample to a first accelerated sedimentation force of at least about 1400 g or greater to form a first processed sample;shipping the first processed sample from a first location to a second location; andsubjecting the first processed sample at the second location to a second accelerated sedimentation force of greater than about 10 g but less than about 500 g.2. (canceled)3. The method of wherein the first processed sample contains a heparin-based anti-coagulant.4. The method of wherein the first processed sample contains a plasma portion separated by a separation gel from a formed-blood component portion.5. The method of wherein the second processed sample does not force electrolytes though the separation gel from the formed-blood component portion to the plasma portion.6. The method of wherein the second processed sample does not force liquid though the separation gel from the formed-blood component portion to the plasma portion.7. The method of wherein the first accelerated sedimentation force is provided through centrifugation.8. The method of wherein the second accelerated sedimentation force is provided through centrifugation.9. The method of wherein a plurality of samples are shipped from a ...

Подробнее
25-01-2018 дата публикации

Plasma spectroscopic analysis method and plasma spectroscopic analyzer

Номер: US20180024069A1
Автор: Tsuyoshi Takasu
Принадлежит: Arkray Inc

A plasma spectroscopic analysis method includes a concentration process including a voltage application period in which voltage is applied to a pair of electrodes in the presence of a sample thereby concentrating an analyte contained in the sample in the vicinity of at least one of the pair of electrodes; and a detection process of generating a plasma by applying voltage to the pair of electrodes and detecting light emitted by the analyte due to the plasma. An electric current between the pair of electrodes is constant while applying voltage for at least a part of the duration of the concentration process.

Подробнее
24-01-2019 дата публикации

Particle detection system and method

Номер: US20190025165A1
Принадлежит: Kanomax Fmt Inc

A particle detector for rapidly detecting and identifying sub 20 nm particles in Ultra Pure Water (UPW) is disclosed. The detector has a nano particle extractor, a nanoparticle collector, and a tracer particle introducer. The extractor limits the size of droplets output to a predetermined size. The extractor includes (1) a liquid sample inlet, (2) a nebulizer connected to the liquid sample inlet (the nebulizer has a gas supply, and an outlet), (3) an impactor arranged to receive material output from the nebulizer, (4) an evaporator connected to the nebulizer and impactor for providing an aerosol at the extractor outlet, and (5) an aerosol connected to the evaporator. The collector us connected to the extractor and has: (1) a collector inlet connected to the aerosol outlet of the extractor, (2) a vapor condensation growth tube connected to the collector inlet, and (3) a repositionable particle capture plate arranged to receive material output from the growth tube at spatially varying positions. The tracer particle introducer is connected to the liquid sample inlet of the extractor. It includes a tracer particle supply connected to a pump which is connected to the extractor. A method for rapid identification of sub −20 nm particles in UPW is also disclosed.

Подробнее
24-01-2019 дата публикации

Methods for Determining Transition Metal Compound Concentrations in Multicomponent Liquid Systems

Номер: US20190025200A1
Автор: QING Yang, Richard M. Buck
Принадлежит: Chevron Phillips Chemical Co LP

Methods for determining the concentration of transition metal compounds in a solution containing more than one transition metal compound are described. Polymerization reactor systems providing real-time monitoring and control of the concentrations of the transition metal components of a multicomponent catalyst system are disclosed, as well as methods for operating such polymerization reactor systems and for improving methods of preparing the multicomponent catalyst system.

Подробнее
28-01-2021 дата публикации

Portable multimode reverse osmosis water purification system

Номер: US20210024381A1
Автор: Clinton W. Hulme
Принадлежит: Mar Cor Purification Inc

A water purification system is disclosed which, includes a reverse osmosis (RO) system or component that is connectable to a city or other outside water feed that is capable of responding to and compensating for low or no feed water pressure coming into the RO system to ensure the outgoing supply of purified water is provided consistently and at a minimum water pressure. This can be accomplished without the need for communication with another device or system-wide facility, such as a hospital, or a pharmaceutical or semiconductor manufacturing system, requiring a constant water supply.

Подробнее
24-01-2019 дата публикации

DETERMINATION OF ORGANIC SILICON IN HYDROCARBONACEOUS STREAMS

Номер: US20190025220A1
Принадлежит:

Systems and methods are provided for determining the organic silicon content of petroleum fractions, such as refinery fractions. This can be achieved in part based on performing solvent-enhanced selective filtration on a hydrocarbonaceous sample to substantially remove inorganic silicon from the sample while retaining at least a substantial portion of the organic silicon in the sample. After the solvent-enhanced selective filtration, the organic silicon content of the filtered sample can be determined. The ability to determine the organic silicon content of a sample can be used to identify crude fractions and/or refinery fractions that may cause contamination problems within a refinery while reducing or minimizing the occurrence of false positive tests that could result from detection of inorganic silicon. 1. A method for determining the silicon content of a hydrocarbonaceous sample , comprising:mixing a hydrocarbonaceous sample with an aromatic solvent to form a mixture, the mixture comprising about 20 wt % to about 80 wt % of an aromatic solvent relative to a weight of the mixture;performing a solids removal process on the mixture suitable for removing particles having a particle size of about 1.0 μm or larger to form a reduced solids mixture; andcharacterizing the silicon content of the hydrocarbonaceous sample using a detection method comprising inductively coupled plasma.2. The method of claim 1 , wherein characterizing the silicon content of the hydrocarbonaceous sample comprises:separating a first fraction comprising a majority of the aromatic solvent and a second fraction comprising a majority of the silicon content from the reduced solids mixture; andcharacterizing the silicon content of the second fraction.3. The method of claim 2 , wherein separating a first fraction comprising a majority of the aromatic solvent from the reduced solids mixture comprises performing a separation based on distillation claim 2 , evaporation claim 2 , or a combination thereof. ...

Подробнее
29-01-2015 дата публикации

Collection Tubes Apparatus, Systems, and Methods

Номер: US20150027957A1
Автор: Emerson Jane F.
Принадлежит:

Methods of producing collection tubes are presented. The methods include providing a separator substance that can rapidly polymerize in a short time to a desired hardness and disposing the separator substance within the lumen of the tube. The separator substance is formulated to have a density between an average density of a serum fraction of whole blood and a cell-containing fraction of whole blood, and to be flowable with whole blood. Upon centrifugation of a tube having blood, the separator substance forms a barrier between the whole blood fractions. The tube and barrier maintain stability of one or more analyte levels, including potassium and glucose, within 10% of their initial values before centrifugation for at least four days. 1. A sample collection tube , comprising:a tube having a lumen;a first separator substance disposed within the lumen,wherein the first separator substance is formulated to isolate a first phase of a sample of whole blood from a second phase of the sample of whole blood upon centrifugation, the first separator substance comprising a curable compound with a density between an average density of the first phase and the second phase and having sufficient reactive groups to form a first solid crosslinked composition within ten minutes of exposure to a suitable energy source; andwherein the first solid crosslinked composition, upon formation, provides a solid barrier seal between the first phase and the second phase.2. The tube of claim 1 , further comprising a second separator substance formulated to migrate between a third phase of the sample of whole blood and at least one of the first phase and the second phase claim 1 , wherein the second separator substance comprises a curable compound having sufficient reactive groups to form a second solid crosslinked composition between the third phase and the at least one of the first phase and the second phase within ten minutes of exposure to a suitable energy source.3. The tube of claim 1 , ...

Подробнее
23-01-2020 дата публикации

Automated Protein Precipitation and/or Dispersive Solid Phase Extraction Using Filter Tips

Номер: US20200025755A1
Принадлежит:

Devices and methods for performing pre-analysis sample processing of biological and chemical samples using robotic liquid handlers are disclosed. Methods for solid phase extraction, protein precipitation and filtration of biological and chemical samples using automation and the devices in a rapid and convenient way are described. 1. A method of automated filtering of a solution , comprising:a) introducing a sample solution comprising at least one target compound into a first sample well on a robotic liquid handler sample tray;b) aspirating said sample solution from said first sample well into a top pipette tip;c) moving said top pipette tip to a pipette tray having at least one filter pipette tip, wherein the filter pipette tip contains at least one screen or porous frit inside the filter pipette top located at a distal delivery end opposite of a hub;d) inserting top pipette tip containing said sample solution into said filter pipette tip, such that an air tight seal is made at or below said hub of said filter pipette tip; ande) dispensing said sample solution through said filter pipette tip into a second sample well to form a filtered solution.2. The method of claim 1 , further comprising step f) extracting said filtered solution using solid phase or liquid-liquid extraction.3. The method of claim 1 , further comprising step f) injecting said filtered solution into an analytical instrument.4. The method of claim 1 , further comprising step f) extracting said filtered solution using solid phase or liquid-liquid extraction claim 1 , and step g) injecting said extracted filtered solution into an analytical instrument.5. The method of claim 1 , wherein said filter pipette tip contains a substrate above said screen or porous frit claim 1 , wherein said substrate is chosen from a group comprising resin claim 1 , polymeric sorbent claim 1 , glass wool claim 1 , fibrous material claim 1 , silica or combinations thereof.6. The method of claim 1 , wherein said filter pipette ...

Подробнее
24-04-2014 дата публикации

System and Method for Charging Fluids

Номер: US20140111901A1
Принадлежит: Stokes Bio Ltd

Devices, systems, and methods for charging fluids are disclosed. The charging of fluids improves the mixing of fluids in microfluidic systems. The charging is performed by producing an ion field ( 50 ) between an ionizing electrode ( 20 ) and an opposed ground electrode ( 30 ). A fluid-containing vessel ( 40 ) is positioned between the opposed electrodes and the ion field charges the fluid ( 41 ) in the vessel.

Подробнее
28-01-2021 дата публикации

USE OF HOLLOW FIBERS TO OBTAIN BLOOD OR A BLOOD DERIVATIVE IMPOVERISHED FROM BLOOD CELLS AND PLATELETS DERIVED EXTRACELLULAR VESICLES

Номер: US20210025795A1
Принадлежит:

A method is provided for using hollow fibers having a porosity above 20 nm, in particular polyethersulfone hollow fibers, to impoverish blood and blood-derivatives from blood-derived extracellular vesicles, in particular exosomes and exomers. Methods for obtaining and analyzing the impoverished samples are also provided. 1. A method for using hollow fibers , the method comprising impoverishing , using hollow fibers having a porosity above 20 nm , by gravity filtering , a blood or blood-derived liquid sample from extracellular vesicles , the extracellular vesicles having a size that is inferior to the porosity of the hollow fibers and originating from blood components selected from the group consisting of blood red cells , blood white cells , platelets , and combinations thereof.2. The method of claim 1 , wherein the hollow fibers comprise polyethersulfone hollow fibers.3. The method of claim 1 , wherein the hollow fibers are polyethersulfone hollow fibers.4. The method of claim 1 , wherein the extracellular vesicles are selected from the group consisting of microvesicles claim 1 , exosomes claim 1 , exomers claim 1 , and combinations thereof.5. The method of claim 1 , wherein the porosity of the hollow fibers is of about 200 nm.6. The method of claim 1 , wherein the porosity of the hollow fibers is comprised between about 150 and about 500 nm.7. The method of claim 1 , wherein the blood-derived liquid sample is a plasma sample.8. A method for obtaining blood or a blood derivative impoverished in extracellular vesicles originating from blood components selected from the group consisting of red blood cells claim 1 , white blood cells claim 1 , platelets claim 1 , and combination thereof claim 1 , the method comprising gravity filtering a blood or blood-derived liquid sample through a filter comprising hollow fibers having a porosity above 20 nm claim 1 , thereby obtaining blood or a blood derivative impoverished in extracellular vesicles claim 1 , the extracellular ...

Подробнее
04-02-2016 дата публикации

Blood separation container for extracting self-platelet

Номер: US20160030661A1
Автор: Eui Jae HWANG
Принадлежит: GOOD MORNING BIO CO Ltd

The present invention relates to a blood separation container for extracting self-platelet. According to the present invention, since a second coupling portion is formed at a release prevention member coupled to a lower portion of a main body in a PRP separation container, and an ascending member having a bolt structure is screwed to the second coupling portion to ascend a lower cover disposed in a lower fluid chamber of the main body, a separate second main body is unnecessary unlike the related art. Therefore, the PRP separation container is easy to carry, manufacturing costs can be reduced to thereby ensure competitive price, and a PRP can be easily separated and extracted.

Подробнее
29-01-2015 дата публикации

Devices, Apparatus, Kit and Method for Treating a Biological Sample

Номер: US20150031040A1
Принадлежит: SILICON BIOSYSTEMS S.P.A.

Method for the treatment of a biological sample comprising at least one cell and a liquid component; according to the method, a force is applied to the sample inserted in an inner chamber of a hollow device towards a filter which has pores with diameters from 2 nm to 1 μm, so that at least part of the liquid component passes through the filter and the cell remains in the inner chamber, thus obtaining a concentrated sample; the filter has a surface facing the inner chamber of less than 12.6 mm. 1. A hollow device for treating a biological sample (A; B) , in particular for separating at least a particle (C) and at least part of a liquid (D) from each other;{'b': 2', '3', '6', '5', '6', '3', '7, 'sup': '2', 'the hollow device () comprises an inner chamber (), which has a volume up to 2 mL; a first end (); a first opening (), which is arranged in the area of the first end (), establishes a communication between the outside and the inner chamber () and has an area of at least 9 mm; and a second end ();'}{'b': 3', '9', '7', '3', '3, 'sup': '2', 'the hollow device () is characterised in that it comprises a filter (), which is arranged in the area of the second end (), separates the inner chamber () from the outside, has pores with diameters ranging from 2 nm to 1 μm, an area (S) facing the inner chamber () up to 12.6 mm, and a thickness up to 500 μm.'}293. A hollow device according to claim 1 , wherein the filter () has a hold-up volume lower than 2 μL and an area (S) facing the inner chamber () of at least 0.1 mm.34310510910439. A hollow device according to claim 1 , and comprising at least one wall () claim 1 , which delimits the inner chamber () and has a second opening () opposite to the first opening () claim 1 , which second opening () has an area from 0.2 mmto 13 mm; the filter () having a thickness from 1 μm to 250 μm and covering in a substantially complete manner the second opening (); the wall () having a thermal conductivity from 0.08 W/mK to 0.7 W/mK and a ...

Подробнее
04-02-2016 дата публикации

CENTRIFUGE CONFIGURATIONS

Номер: US20160032361A1
Принадлежит:

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. 130-. (canceled)31. A sample processing device for use with a sample vessel , the device comprising:a housing;a programmable fluid handling system within said housing;a cartridge receiving area at a first location in the housing accessible by the programmable fluid handling system;at least one optical detector positioned at a second location in the housing accessible by the programmable fluid handling system;a nucleic acid amplification station at a third location in the housing accessible by the programmable fluid handling system; and{'sup': '2', 'a centrifuge positioned at a fourth location in the housing accessible by the programmable fluid handling system, wherein the centrifuge comprises: a motor assembly comprising a rotor configured to rotate about a stator about an axis of rotation, wherein the motor assembly has a height that is oriented along a direction of the axis of rotation, wherein along said height said stator is circumscribed by said rotor; and a base affixed to the rotor, wherein the base rotates about the stator, wherein the base comprises a bucket that is characterized in that (a) the bucket comprises a cavity to receive the sample vessel and (b) the bucket is configured to pivot about a pivot axis to move the cavity from a first orientation when the base is at rest to a second orientation when the base is rotating, wherein the centrifuge is capable of providing G-forces of up to about 1200 m/s.'}32. The centrifuge of claim 31 , wherein the cavity is configured to ...

Подробнее
31-01-2019 дата публикации

BLOOD AND BIOLOGICAL SAMPLE COLLECTION DEVICE AND METHOD

Номер: US20190029581A1
Принадлежит: Sedia Biosciences Corporation

Specially designed collection strips and their processing. By using specially designed collection strips, having a backer and one or more absorbent pads, in conjunction with a unique processing method, the processes of analyzing biological samples such as blood, or the like, may be done efficiency with the elimination of cross contamination risk. Identification of the sample stays with the sample throughout the process as it resides on the collection strip. The strip absorbs a known volume. The sample with identification is placed directly in an elution solution, without mechanically separating the sample from its identification information. Elimination of the need for mechanical separation tends to reduce cross contamination, as well as reducing sample processing time. 1. A blood collection device that does not require punching , or folding to obtain a sample for processing comprising:a flat rectangular elongated backer having a first end and a second end;a planar label area disposed on the first end upon which identifying information of a collected sample is recorded; anda separate absorbent pad attached at the second end of the flat rectangular elongate backer that does not require punching to obtain a sample whereby a blood sample is applied to the absorbent pad and dried for later testing, the construction of the blood collection device not requiring a folding, a punching out, or detachment of a portion of the blood collection device containing the blood sample, and whereby the identifying information of the collected sample stays affixed to the sample during processing of the sample.2. The blood collection device of claim 1 , in which the absorbent pad is cellulose.3. The blood collection device of claim 1 , in which the absorbent pad is polyester.4. The blood collection device of claim 1 , in which the absorbent pad is glass fiber.5. The blood collection device of claim 1 , in which the absorbent pad is treated with a detergent.6. The blood collection device ...

Подробнее
02-02-2017 дата публикации

Biofluid Collection and Filtration Device

Номер: US20170030811A1
Принадлежит: CellectGen, LLC

The present specification discloses filtration devices for processing a biofluid sample, methods of purifying a biofluid sample using the disclosed filtration devices, and systems comprising components of the filtration devices. 1. A filtration device comprisinga) a collection container comprising a collection chamber and a filter device comprising a filter mount, a filter, and O-ring; and 'wherein the quantitative container is removably attached to the collection container.', 'b) a quantitative container comprising a quantitative chamber;'}2. The filtration device according to claim 1 , wherein the filter device further comprises a port and a one-way check valve.3. The filtration device according to claim 1 , wherein the quantitative container further comprises a base.4. The filtration device according to claim 3 , wherein the base is removable.5. The filtration device according to claim 1 , wherein the collection container is designed to have flexible walls.6. The filtration device according to claim 1 , wherein the collection container is designed to have rigid walls.7. The filtration device according to claim 1 , wherein the quantitative container is configured to move into the collection chamber upon the application of force.8. The filtration device according to claim 1 , further comprising a plunger device claim 1 , wherein the plunger device is removably attached to the quantitative container and wherein the plunger device is configured to move into the quantitative chamber upon the application of force.9. The filtration device according to claim 1 , wherein the plunger device comprises a plunger including a channel claim 1 , a valve claim 1 , and a plunger O-ring claim 1 , and a plunger attachment including a plunger attachment face seal.10. The filtration device according to claim 1 , wherein the collection container includes a collection chamber cap attached to the collection container.11. The filtration device according to claim 1 , wherein the collection ...

Подробнее
01-02-2018 дата публикации

NUCLEIC ACID AMPLIFICATION REACTION METHOD, REAGENT SET, AND METHOD OF USING REAGENT SET

Номер: US20180030508A1
Принадлежит:

A nucleic acid amplification reaction method includes a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer, a temperature increasing step of increasing the temperature of the reaction solution to a temperature at which the amplified nucleic acid is denatured after the thermal cycling step, a temperature decreasing step of decreasing the temperature of the reaction solution to a temperature at which a probe hybridizes after the temperature increasing step, and a probe addition step of adding the probe to the reaction solution. 1. A nucleic acid amplification reaction method , comprising:a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer;a temperature increasing step of increasing the temperature of the reaction solution to a temperature at which the amplified nucleic acid is denatured after the thermal cycling step;a temperature decreasing step of decreasing the temperature of the reaction solution to a temperature at which a probe hybridizes after the temperature increasing step; anda probe addition step of adding the probe to the reaction solution, whereinin the thermal cycling step,the time per cycle of the thermal cycling is 9 seconds or less,the Tm value of the primer is 70° C. or higher and 80° C. or lower,the probe addition step is performed after the thermal cycling step and before the temperature increasing step, or after the temperature increasing step and before the temperature decreasing step, or after the temperature decreasing step.2. The nucleic acid amplification reaction method according to claim 1 , wherein the time per cycle of the thermal cycling is 2.5 seconds or less.3. The nucleic acid amplification reaction method according to claim 1 , wherein the probe contains a minor groove binder molecule.4. The nucleic acid amplification reaction method according to claim 1 , whereinthe reaction solution ...

Подробнее
01-02-2018 дата публикации

NUCLEIC ACID AMPLIFICATION REACTION METHOD, REAGENT, AND METHOD OF USING REAGENT

Номер: US20180030509A1
Принадлежит:

A nucleic acid amplification reaction method includes a heating step of heating a reaction solution containing a reverse transcriptase, a polymerase, a primer, and a probe for performing a reverse transcription reaction, and a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for the reaction solution after the heating step, wherein the Tm value of the primer is 65° C. or higher and 80° C. or lower, and in the heating step, a heating time for the reaction solution is 5 seconds or more and 480 seconds or less. 1. A nucleic acid amplification reaction method , comprising:a heating step of heating a reaction solution containing a reverse transcriptase, a polymerase, a primer, and a probe for performing a reverse transcription reaction; anda thermal cycling step of performing thermal cycling for amplifying a nucleic acid for the reaction solution after the heating step, whereinthe Tm value of the primer is 65° C. or higher and 80° C. or lower, andin the heating step,a heating time for the reaction solution is 5 seconds or more and 480 seconds or less.2. The nucleic acid amplification reaction method according to claim 1 , wherein in the heating step claim 1 , the reaction solution is heated to 50° C. or higher and 70° C. or lower.3. The nucleic acid amplification reaction method according to claim 2 , wherein in the heating step claim 2 , a heating time for the reaction solution is 10 seconds or more and 60 seconds or less.4. The nucleic acid amplification reaction method according to claim 1 , wherein the amount of the reverse transcriptase contained in the reaction solution is 30 units or more and 60 units or less.5. The nucleic acid amplification reaction method according to claim 4 , wherein in the heating step claim 4 ,the reaction solution is heated to 60° C. or higher and 70° C. or lower, anda heating time for the reaction solution is 10 seconds or more and 30 seconds or less.6. The nucleic acid amplification reaction method ...

Подробнее
01-02-2018 дата публикации

NUCLEIC ACID AMPLIFICATION REACTION METHOD AND NUCLEIC ACID AMPLIFICATION REACTION REAGENT

Номер: US20180030510A1
Принадлежит:

A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer and a probe, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, and the Tm value of the primer is 70° C. or higher and 80° C. or lower. 1. A nucleic acid amplification reaction method , comprising:performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer and a probe, whereinin the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, andthe Tm value of the primer is 70° C. or higher and 80° C. or lower.2. The nucleic acid amplification reaction method according to claim 1 , wherein a heating time for an annealing reaction for the primer is 6 seconds or less.3. The nucleic acid amplification reaction method according to claim 1 , whereinthe reaction solution contains a divalent cation, andthe concentration of the divalent cation contained in the reaction solution is 2 mM or more and 7.5 mM or less.4. The nucleic acid amplification reaction method according to claim 3 , wherein{'sub': '2', 'the reaction solution contains MgCl,'}{'sub': '2', 'the divalent cation is derived from MgCl, and'}{'sub': '2', 'the concentration of MgClcontained in the reaction solution is 4 mM or more and 7.5 mM or less.'}5. The nucleic acid amplification reaction method according to claim 3 , wherein{'sub': '4', 'the reaction solution contains MgSO,'}{'sub': '4', 'the divalent cation is derived from MgSO, and'}{'sub': '4', 'the concentration of MgSOcontained in the reaction solution is 2 mM or more and 3 mM or less.'}6. The nucleic acid amplification reaction method according to claim 1 , wherein the Tm value of the primer is 70° C. or higher and 75° C. or lower.7. The nucleic acid amplification reaction method according to claim 1 , wherein the primer contains an artificial nucleic acid.8. The nucleic acid amplification ...

Подробнее