Microfluidic Sensing

A device including a microfluidic channel structure formed on a substrate and including a first channel and a fluid actuator within the microfluidic channel structure. A sense region within the first channel is to receive a fluid flow of target biologic particles for counting in a single file pattern, with the sense region having a volume on a same order of magnitude as a volume of a single one of the target biologic particles. 1. A biologic test chip comprising:a substrate;a microfluidic channel structure formed on the substrate and including a first channel;a fluid actuator within the microfluidic channel structure.a sense region within the first channel to receive a fluid flow of biologic particles on a one-at-a-time basis via operation of the fluid actuator, the sense region having a volume on a same order of magnitude as a volume of a single respective one of the biologic particles.2. The chip of claim 1 , wherein the sense region operates according to a volume fraction in which a ratio of the volume of each single biologic particle relative to the volume of the sense region is on an order of tenths.3. The chip of claim 2 , comprising:at least one impedance sensor generally coextensive within the sense region to count biologic particles passing through the sense region.4. The chip of claim 3 , wherein the biologic particles are subject to a dilution factor on the order of tens.5. The chip of claim 4 , wherein the channel structure provides a non-uniform flow portion to align the biological particles into a single file flow pattern through the sense region claim 4 , the non-uniform flow portion including at least one of:an exclusion structure upstream from the sense region to exclude biologic particles larger than the volume of the sense region; andan inlet including a progressively narrowing cross-sectional area in the downstream orientation.6. The chip of claim 5 , wherein the first channel generally defines a first cross-sectional area and the first channel ...

DOWNHOLE LOCAL SOLID PARTICLES COUNTING PROBE, PRODUCTION LOGGING TOOL COMPRISING THE SAME AND SAND ENTRY INVESTIGATION METHOD FOR HYDROCARBON WELLS

A downhole local solid particles counting probe () for counting solid particles () in a fluid () present in a hydrocarbon well in production comprising: 1. A downhole local solid particles counting probe for counting solid particles in a fluid present in a hydrocarbon well in production comprising:an elongated and flexible protective tube defining an internal cavity terminating by a membrane wall defining a tip, the protective tube and the membrane wall isolating the internal cavity from the fluid of the hydrocarbon well, the protective tube and membrane wall are made of metal or metal alloy and have a thickness such as to resist to a downhole hydrocarbon well pressure;a passive acoustic sensor mounted inside the internal cavity, the passive acoustic sensor having a front side mechanically coupled on the membrane wall of the tip;wherein:a characteristic dimension of the passive acoustic sensor is similar to solid particles average characteristic dimension, ranging from 0.5 mm to 1.5 mm, and a characteristic dimension of the membrane wall defining the tip ranges from 1 mm to 2 mm;the passive acoustic sensor is arranged to detect acoustic waves generated by solid particles impacting the membrane wall defining the tip so as to resolve an individual impact from a single solid particle and to produce a signal representative of a count of solid particles.2. The probe of claim 1 , wherein the internal cavity is under a pressure ranging from 0 to 4 atm.3. The probe according to claim 1 , wherein the passive acoustic sensor has a disk shape claim 1 , and the protective tube claim 1 , internal cavity and membrane wall have a cylindrical shape.4. The probe according to claim 1 , wherein the passive acoustic sensor is a piezoelectric ceramic.5. The probe according to claim 1 , wherein the protective tube and the membrane wall are made of austenite nickel-chromium-based super-alloys.6. The probe according to claim 4 , wherein the piezoelectric ceramic comprises metallization ...

Methods for predicting overall and progression free survival in subjects having cancer using circulating cancer associated macrophage-like cells (camls)

Means for predicting overall survival (OS) and progression free survival (PFS) of subjects having cancer are disclosed, where the predictions are based on the number arid size of circulating cancer associated macrophage-like cells (CAMLs) found in a biological sample, such as blood, from the subject.

Particle Detection Methods and Systems for Practicing Same

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided. 123-. (canceled)24. A method for detecting cells in a flow cytometer , comprising:flowing a cellular sample comprising cells through a flow cell of a flow cytometer;detecting optical signals from the cells flowing through the flow cell at a first gain setting; anddetecting optical signals from the cells flowing through the flow cell at the second gain setting, wherein the second gain setting is different from the first gain setting.25. The method according to claim 24 , wherein the second gain setting is greater than the first gain setting.26. The method according to claim 25 , wherein the second gain setting is to account for the cellular sample having a high cell concentration.27. The method according to claim 24 , wherein the second gain setting is less than the first gain setting.28. The method according to claim 27 , wherein the second gain setting is to account for the cellular sample having a low cell concentration.29. The method according to claim 24 , wherein the first and second gain settings comprise a photo diodes gain setting claim 24 , a photo multiplier tubes (PMT) gain setting claim 24 , or both.30. The method according to claim 24 , wherein the cellular sample is a blood sample.31. The method according to claim 24 , comprising:analyzing the optical signals detected at the first gain setting to detect a first cell type; andanalyzing the optical signals detected at the second gain setting to detect a second cell type.32. The method according to claim 31 , wherein the first gain setting is higher than the second gain setting claim 31 , and wherein platelets are detected at the ...

DIAGNOSTIC CHIP

A microfluidic diagnostic chip may comprise a main fluid channel comprising a main pump, a secondary fluid channel branching off from the main fluid channel, and a secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes. A method of analyzing an analyte on a microfluidic chip may comprise pumping, with a main microfluidic pump, a fluid comprising an analyte particle through a main microfluidic channel fluidly coupled to a fluid slot and sorting the analyte particle within the fluid through a secondary microfluidic channel by pulling the analyte particle into the secondary microfluidic channel with a secondary microfluidic pump. 1. A microfluidic diagnostic chip , comprising:a main fluid channel comprising a main pump;a secondary fluid channel branching off from the main fluid channel; anda secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes.2. The microfluidic diagnostic chip of claim 1 , wherein the secondary fluid channel comprises a smaller diameter than the main fluid channel.3. The microfluidic diagnostic chip of claim 2 , wherein the secondary fluid channel comprises a first sensor to count a number of the particles of the first size passing therethrough.4. The microfluidic diagnostic chip of claim 2 , wherein the diameter of the secondary fluid channel excludes the number of larger sizes of particles.5. The microfluidic diagnostic chip of claim 3 , wherein the main fluid channel comprises a second sensor to count particles of the analyte of the first size and larger sizes passing therethrough.6. The microfluidic diagnostic chip of claim 5 , ...

Methods and Apparatus for Real-Time Detection and Clearing of a Clog

A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

TREATMENT OF CANCERS USING PI3 KINASE ISOFORM MODULATORS

Provided herein are methods, kits, and pharmaceutical compositions that include a PI3 kinase inhibitor for treating cancers or hematologic disorders. 138-. (canceled)40. The method of claim 39 , wherein the hematologic malignancy has a high expression level of PI3K-δ claim 39 , or PI3K-γ claim 39 , or both PI3K-δ and PI3K-γ.41. The method of claim 39 , wherein the compound or pharmaceutically acceptable salt claim 39 , solvate claim 39 , hydrate claim 39 , co-crystal claim 39 , clathrate claim 39 , or polymorph thereof is administered in an amount sufficient to provide a plasma concentration of the compound at steady state at a level higher than the ICfor PI3K-δ and at a level higher than the ICfor PI3K-γ.42. The method of claim 39 , wherein the compound or pharmaceutically acceptable salt claim 39 , solvate claim 39 , hydrate claim 39 , co-crystal claim 39 , clathrate claim 39 , or polymorph thereof is administered in an amount sufficient to provide a plasma concentration of the compound at steady state of 350 ng/ml to 450 ng/ml.43. The method of claim 39 , wherein the hematologic malignancy is chronic lymphocytic leukemia (CLL) claim 39 , indolent non-Hodgkin lymphoma (iNHL) claim 39 , small lymphocytic lymphoma (SLL) claim 39 , CLL/SLL claim 39 , or follicular lymphoma.44. The method of claim 39 , wherein the hematologic malignancy is relapsed or refractory.45. The method of claim 43 , wherein the hematologic malignancy is relapsed or refractory.46. The method of claim 39 , wherein the hematologic malignancy is refractory to rituximab therapy claim 39 , chemotherapy claim 39 , and/or radioimmunotherapy.47. The method of claim 39 , wherein the PI3K inhibitor is administered in combination with a second active agent selected from the group consisting of a BCL-2 inhibitor claim 39 , an HDAC inhibitor claim 39 , a MEK inhibitor claim 39 , an EZH2 inhibitor claim 39 , a BTK inhibitor and a PLK-1 inhibitor.48. The method of claim 47 , wherein the second active agent is ...

Systems and Methods for the Detection of Biomolecules

A system and method for the detection and quantification of biomolecules by measuring a piezoelectric signal is described. The system comprises a plurality of elongate zinc oxide nanowires mounted generally parallel to each another on a semi conductive silicon substrate. The free ends of the nanowires are provided with biomolecules that are capable of associating with complementary biomolecules within a biological or water sample. Following incubation of the system in a sample, the association of molecules of interest with the immobilised biomolecules on the system results in the displacement of the zinc oxide nanowires. The displacement of the nanowires produces a piezoelectric voltage signal that is useful in diagnosing a pathogenic infection or the contamination of a sample. 1. A system for the detection of biomolecules , comprising a plurality of piezoelectric nanowires having ends mounted on a semi conductive substrate and opposite free ends extending generally parallel in a direction substantially perpendicular to the semi conductive substrate , wherein each nanowire has biomolecules immobilised onto at least a portion of a surface of a its free end of said each nanowire , wherein base portions of the nanowires are coated with an insulating layer of material which fills the spaces between the nanowires whilst the free ends remain substantially uncoated and uninsulated , and wherein displacement of the nanowires owing to the association of a second type of biomolecule with the biomolecules immobilised on the free ends produces a piezoelectric signal.2. (canceled)3. The system of claim 1 , wherein the semi conductive substrate comprises silicon wafers wherein claim 1 , a first section of a surface of the silicon wafers is coated or partially coated with a layer of titanium or titanium oxide claim 1 , the titanium/titanium oxide-coated silicon wafers are coated with a conductive layer claim 1 , a zinc oxide (ZnO) seed layer is provided on the conductive layer so ...

ULTRASOUND IMAGE-BASED CONCENTRATION MEASUREMENT

The systems and methods of the present disclosure are directed to ultrasound-based concentration measurement techniques in which both scatterer count and image volume are measured concurrently to provide absolute concentration measurements. In particular, through the techniques of the present disclosure, the effective thickness of an ultrasound beam can be determined based on the spreading of individual scatterers within ultrasound images. Based on the effective thickness of the ultrasound beam, the volume of the image and, thus, the concentration of particles in the image can be determined directly, without the need for estimation, approximation, or use of a reference sample. 1. A method comprising:acquiring an ultrasound image of a medium with an ultrasound transducer, wherein the ultrasound image includes at least a portion of a two-dimensional image obtained from the ultrasound transducer and wherein the medium contains a number of scatterers;determining an effective volume of the ultrasound image in which one or more of the number of scatterers in the medium produce an echo detectable within the two-dimensional image;counting the scatterers in the ultrasound image; andbased on the counted scatterers and the effective volume of the ultrasound image, determining an absolute concentration of the scatterers in the medium.2. The method of claim 1 , wherein determining the absolute concentration of the scatterers in the medium includes determining the absolute concentration of the scatterers without contacting the medium.3. The method of claim 1 , wherein determining the effective volume of the ultrasound image is based on echogenicity of the scatterers and attenuation of the medium.4. The method of claim 1 , wherein determining the effective volume of the ultrasound image is based on a ratio of an elevational beam profile to a lateral beam profile.5. The method of claim 1 , wherein determining the effective volume of the two-dimensional image includes determining an ...

PREPARATION OF NUCLEATED RBC (NRBC) ANALOGS FOR USE AS REFERENCE HEMATOLOGY CONTROLS IN AUTOMATED HEMATOLOGY ANALYZERS

The subject invention pertains to compositions of novel analogs of red blood cells that are distinguishable from white blood cells in a hematological instrument and processes for manufacturing such analogs. The processes for creating the compositions comprise washing, shrinking, and fixing cells at temperatures at or below room temperature. 1. A process for manufacture of red blood cells which are distinguishable from white blood cells for use as a reference control in a blood cell analyzer , the process comprising the steps of:a) shrinking and washing red blood cells of a non-human vertebrate by application of a salt solution to extract cellular fluid from the red blood cells; andb) applying a quantity of a fixing agent to the red blood cells from step (a), said salt solution comprising a denaturing agent.2. The process of claim 1 , wherein the blood cell analyzer uses optical measurements to distinguish cell types.3. The process of claim 1 , wherein the non-human vertebrate is a chicken.4. The process of claim 1 , wherein the red blood cells are nucleated red blood cells.5. The process of claim 1 , wherein the salt solution comprises a salt and a naphthol or a derivative thereof as the denaturing agent.6. The process of claim 5 , wherein the denaturing agent is α-naphthol.7. The process of claim 5 , wherein the denaturing agent concentration is about 0.1 g/L to about 100 g/L claim 5 , about 1 g/L to about 10 g/L claim 5 , or about 2.5 g/L to about 7.5 g/L or about 4.5 g/L.8. The process of claim 5 , wherein the salt concentration is about 0.1 g/L to about 100 g/L claim 5 , about 1 g/L to about 10 g/L claim 5 , or about 2.5 g/L to about 7.5 g/L.9. The process of claim 1 , wherein the fixing agent is glutaraldehyde.10. The process of claim 1 , wherein the fixing agent concentration is about 0.01% to about 10% claim 1 , about 0.1% to about 1% claim 1 , or about 0.24% to about 0.48%.11. The process of claim 1 , wherein step (a) further comprises cooling the cells to a ...

METHOD OF TREATING PATIENTS WITH HEPATORENAL SYNDROME TYPE 1

The principles and embodiments of the present disclosure relate to methods for using terlipressin to treat a patient having impaired renal function associated with liver disease. A patient identified as suffering from HRS-1 is tested to determine if the patient meets at least two out of three criteria, wherein the three criteria include a WBC<4 or >12 cells/4; HR>90 bpm; and any one of HCO3<21 mmol/L or PaCO2<32 mmHg or >20 breaths per minute. If the patient meets at least two of the criteria, he or she is administered terlipressin in an amount effective to produce a reduction in serum creatinine of at least 1.0 mg/dL. 1. A method of reversing type 1 hepatorenal syndrome (HRS-1) , the method comprising:administering, to a patient having HRS-1 that is Systemic Inflammatory Response Syndrome (SIRS) positive, an amount of terlipressin effective to reverse the HRS-1 in the patient; [{'sup': 3', '3, '(i) a white blood cell count (WBC) less than 4,000 cells/mmor greater than 12,000 cells/mm,'}, '(ii) a heart rate of greater than 90 beats per minute (BPM), and', {'sub': 2', '3, '(iii) either a partial pressure of carbon dioxide in the blood (PaCO) <32 mmHg or a blood bicarbonate (HCO) level <23 mmol/L;'}], 'wherein the patient is SIRS positive by exhibiting at least two of the following three criteriawherein the patient does not have overt sepsis, septic shock, or uncontrolled infection, andwherein administration of terlipressin to the patient produces a decrease in serum creatinine level to ≤1.5 mg/dl and reverses HRS-1.2. The method of claim 1 , wherein the amount of terlipressin administered to the patient is in the range of 2.0 mg to 12.0 mg per day for 1 to 28 days.3. The method of claim 1 , wherein the terlipressin is administered as an IV.4. The method of claim 1 , comprising treating the patient with up to a maximum of 100 g per day of albumin for each day of the time period that the patient is administered terlipressin.5. The method of claim 1 , wherein ...

ELECTRIC-CHARGE GENERATING ELEMENT AND PARTICLE COUNTER

An electric-charge generating element that generates electric charges by gaseous discharge includes a dielectric layer, a discharge electrode disposed on one surface of the dielectric layer, a ground electrode disposed on the other surface or inside of the dielectric layer and a nozzle that is disposed in the dielectric layer at a position such that the nozzle does not interfere with the discharge electrode and the ground electrode so as to penetrate through the dielectric layer. 1. An electric-charge generating element that generates electric charges by gaseous discharge , comprising:a dielectric layer;a discharge electrode disposed on one surface of the dielectric layer;a ground electrode disposed on the other surface or inside of the dielectric layer; anda nozzle that is disposed in the dielectric layer at a position such that the nozzle does not interfere with the discharge electrode and the ground electrode so as to penetrate through the dielectric layer.2. The electric-charge generating element according to claim 1 ,wherein the electric-charge generating element adds the generated electric charges to particles included in a gas.3. The electric-charge generating element according to claim 1 ,wherein the dielectric layer has the nozzle at a center of the dielectric layer.4. The electric-charge generating element according to claim 1 ,wherein an opening shape of the nozzle is a polygon, a circle, or an ellipse.5. The electric-charge generating element according to claim 1 ,wherein the dielectric layer has a cone shape, andwherein the nozzle is disposed at a vertex of the dielectric layer.6. The electric-charge generating element according to claim 1 ,wherein the dielectric layer has a pyramid shape, andwherein the nozzle is disposed at a vertex of the dielectric layer.7. The electric-charge generating element according to claim 5 ,wherein the discharge electrode is disposed on an inner surface of the dielectric layer, andwherein the ground electrode is disposed ...

DEVICE FOR COUNTING PARTICLES

A device for counting particles comprises a detector arranged to produce an electrical measurement signal in response to the passage of one or more particles, and a comparator arranged to compare the measurement signal with a threshold signal and to increment a counting value when the measurement signal exceeds the threshold signal, characterized in that it furthermore comprises a threshold-adjusting circuit that applies a lowpass filter to the measurement signal, and that is connected to the comparator in order to use the resulting signal as threshold signal. 1. A device for counting particles comprisinga detector arranged to produce an electrical measurement signal in response to the passage of one or more particles,a comparator arranged to compare the measurement signal with a threshold signal and to increment a counting value when the measurement signal exceeds the threshold signal, anda threshold-adjusting circuit that applies a lowpass filter to the measurement signal, and that is connected to the comparator in order to use the resulting signal as threshold signal.2. The device of claim 1 , wherein the threshold-adjusting circuit comprises a resistor and a capacitor mounted in series claim 1 , and the comparator being an operational amplifier in comparator mode one input of which receives the measurement signal claim 1 , and the other input of which is connected to the threshold-adjusting circuit between the resistor and the capacitor.3. The device of claim 1 , wherein the comparator is mounted between at least two hysteresis resistors.4. The device of claim 1 , furthermore comprising an analogue-digital converter for digitizing the measurement signal claim 1 , and wherein the comparator and the threshold-adjusting circuit are produced in digital form.51. The device of claim 4 , wherein the threshold-adjusting circuit is a finite-impulse-response filter of order .6. The device of claim 1 , further comprising a circuit ensuring the threshold signal has a ...

Micro-Array Devices for Capturing Cells in Blood and Methods of Their Use

The present disclosure provides micro-array devices for capturing cells in blood and methods of their use. In some aspects, a method for counting cells in a blood sample is provided, the method comprising applying a blood sample onto a CNT device; allowing cells in the blood sample to differentially settle on the CNT device, and identifying and counting cells of preselected type in the blood sample. 1) A method for counting cells in a blood sample comprising:applying a blood sample onto a CNT device;allowing cells in the blood sample to differentially settle on the CNT device, and identifying and counting cells of a pre-selected type in the blood sample.2) The method of wherein the cellular targets are breast cancer cells.3) The method of wherein the CNT device comprises:a substrate;a thin film of carbon nanotubes (CNTs) disposed on the substrate, the CNTS being functionalized with one or more antibodies capable to bind to a cellular target to be captured; anda plurality of conductive contacts disposed on the substrate and electrically coupled to the thin film, wherein the plurality of conductive contacts are configured detect a capture of the cellular targets by the one or more antibodies.4) The method of wherein the film is formed from a single layer of carbon nanotubes.5) The method of wherein the one or more antibodies include with EpCAM and anti-Her2.6) The method of wherein the film includes a passivation layer so only the functionalized carbon nanotubes are exposed to the blood sample.7) The method of wherein the film has a density may be between 3 and 5 nanotubes per micrometer.8) The method of wherein the film is functionalized with 1-Pyrenebutanoic Acid Succinimidyl Ester (PASE) to conjugate one or more antibodies to the film.9) A method for specific detection of cellular targets in the blood sample comprising:applying a blood sample onto a CNT device;receiving an electrical signal from the CNT device, the signal being indicative of interactions between ...

DEVICE AND A METHOD FOR LIGHTING, CONDITIONING AND CAPTURING IMAGE(S) OF ORGANIC SAMPLE(S)

Micro-biological colony counters and more particularly, to a device and a method for lighting, conditioning and capturing image(s) of organic sample(s) such as but not limited to micro-organisms. The device () captures accurate image(s) of organic sample(s) and has a fixed focus imaging for repeatability in quality of images. The device () can capture images of organic sample in different lighting and color conditions thereby improving detection of microbiological colonies by increasing the contrast from the background medium. The color calibrated imaging device () provides diffused illumination by using polychromatic LED lights, light reflectors and light diffusers for optimal color reproduction of micro-biological colonies contained in organic sample(s). The device () is adapted for automatic capturing of images of organic sample(s) cultivated on petri dishes of different sizes.

Method of Identifying Biologic Particles

A method of analysing and identifying particles in an input fluid by electrical field analysis preferably in combination with imaging analysis and towards establishing characterises of the particles that can be compared with characteristics of known particles, preferably biologic particles such as bacteria and viruses. Preferably in a focusing step, the particles are focused as in a microfluidic particle sorting cartridge followed by electrical field analysis to determine impedance data. Preferably, the electrical field analysis is carried out in a water and alcohol solution. 1. A method of identifying biologic particles in an input fluid comprising:a focusing step in which the input fluid is sorted into a focused fluid stream including particles from the input stream in a desired range of particle sizes and/or a desired range of particle shapes,an imaging analysis step of determining with a first electromagnetic imaging apparatus image characteristics of the particles in the focused fluid stream,an electrical field analysis step of determining with an electrical field measurement apparatus electrical field characteristics of the particles in the focused fluid stream,using an image identification method comprising a review of the image characteristics to determine a primary estimated identity of the particles in the focused fluid stream as a primary probability profile of one or more known biologic particles,estimating an estimated concentration of the particles in the focused fluid stream when the electrical field characteristics is determined,using an electrical field identification method to estimate a secondary estimated identity of the particles in the focused fluid stream as a secondary probability profile of one or more known biologic particles based on the determined electrical field characteristics and the estimated concentration, andusing the primary estimated identity and the secondary estimated identity to estimate a final estimated identity of the ...

METHOD OF TREATING PATIENTS WITH HEPATORENAL SYNDROME TYPE 1

The principles and embodiments of the present disclosure relate to methods for using terlipressin to treat a patient having impaired renal function associated with liver disease. A patient identified as suffering from HRS-1 is tested to determine if the patient meets at least two out of three criteria, wherein the three criteria include a WBC<4 or >12 cells/4; HR>90 bpm; and any one of HCO<21 mmol/L or PaCO<32 mmHg or >20 breaths per minute. If the patient meets at least two of the criteria, he or she is administered terlipressin in an amount effective to produce a reduction in serum creatinine of at least 1.0 mg/dL. 1. A method of treating type 1 hepatorenal syndrome (HRS-1) , the method comprising:identifying a plurality of patients as having HRS-1; [{'sup': 3', '3, '(i) a white blood cell count (WBC) less than 4,000 cells/mmor greater than 12,000 cells/mm,'}, '(ii) a heart rate of greater than 90 beats per minute (BPM), and', {'sub': 2', '3, '(iii) either a partial pressure of carbon dioxide in the blood (PaCO)<32 mmHg or a blood bicarbonate (HCO) level<23 mmol/L;'}], 'determining that a first patient of the plurality exhibits at least two of the following three criteriadetermining that, because the first patient exhibits at least two of the three criteria, the HRS-1 of the first patient is likely to respond to treatment with terlipressin;administering to the first patient an amount of terlipressin effective to treat HRS-1 in the first patient;determining that a second patient of the plurality exhibits only one or none of the three criteria;determining that, because the second patient does not exhibit at least two of the three criteria, the HRS-1 of the second patient is unlikely to respond to treatment with terlipressin; andexcluding the second patient from treatment with terlipressin.2. The method of claim 1 , wherein a treatment other than terlipressin is administered to the second patient.3. The method of claim 1 , wherein criterion (iii) is a HCOlevel<23 ...

METHOD FOR DETECTING PARTICLES IN A FLUID STREAM

The invention relates to a method for detecting particles in a fluid stream, comprising: generating a measurement field that can be passed through by the fluid stream; acquiring and evaluating measurement values of the fluid stream passing through the measurement field, and detecting at least one particle by way of a distinctive sequence of measurement values. The invention is characterized in that each of the distinctive successions of measurement values is acquired and evaluated in order to determine if a particle or a gas bubble is passing through the measurement field. 1. A method for detecting particles in a fluid stream , comprising:generating a measurement field which may be passed through by a fluid stream,recording and evaluating measurement values in the fluid stream passing the measuring field, anddetecting at least one particle, in each case, by means of a characteristic sequence of measurement values,characterized in that the characteristic sequence of measurement values is recorded and evaluated in terms of whether a particle or gas bubble is passing the measurement field.2. The method according to claim 1 , characterized in that the measurement values in each case are recorded and evaluated for an observation period claim 1 , which is greater than the measurement duration of a sequence of measurement values characteristic of one particle and/or of one glass bubble.3. The method according to claim 2 , characterized in that the observation period is at least double claim 2 , preferably 3.5 times the corresponding measurement duration.4. The method according to claim 1 , characterized in that the sequence of measurement values characteristic of one particle has a particular signal shape claim 1 , and in that another sequence of measurement values characteristic of a gas bubble has another signal shape.5. The method according to claim 4 , characterized in that the signal shape for a particle and the other signal shape for a gas bubble at least partially ...

MICROFLUIDIC DEVICE FOR FULL BLOOD COUNT

A microfluidic device () for full blood count includes a first measurement channel () and a second measurement channel () separated from the first measurement channel (). The microfluidic device () furthermore includes a first inlet () for providing a whole blood sample to the first and second measurement channel (), a second inlet () for providing a lysis agent for white blood cell count in to the first channel (), a third inlet () for providing a quench solution to the first channel (), and a fourth inlet () for providing a lysis agent for hemoglobin measurement to the second channel (). A method for forming such a microfluidic device () and a method for performing a full blood count test using such a microfluidic device () are described. 1. A method for manufacturing a microfluidic device for full blood count , the method comprising:providing a first measurement channel,providing a second measurement channel the second measurement channel separated from the first measurement channel,providing a first inlet for providing a whole blood sample to the first and second measurement channel,providing a second inlet at the first measurement channel for providing a lysis agent for white blood cell count in to the first channel,providing a third inlet at the first measurement channel for providing a quench solution to the first channel, andproviding a fourth inlet at the second measurement channel for providing a lysis agent for hemoglobin measurement to the second channel.2. A method for performing full blood count , the method comprising:providing a blood sample to a first and a second measurement channel of a microfluidic device, the first and second measurement channel being separated from each other,providing a lysis agent suitable for white blood cells to the blood sample in the first channel,providing a quench solution to the blood sample in the first channel,providing a lysis agent for hemoglobin to the blood sample in the second channel,at the end of the first ...

LEUKOCYTE QUANTITATION MICROFLUIDIC METHOD AND DEVICE

A microfluidic solenoid point of use device for discrete quantitation of magnetized cells. The inventive device provides higher accuracy, lower cost, and less bulk than other counting devices. 1. A quantitation device for a target cell population , the device comprising housing a conductive vertical integrated array (via) for the discrete passage of the target cell population therethrough, and', 'operatively contacting a plurality of metal coils by wrapping the coils around the solenoid and securing the metal coils to the conductive via by a conductive wire, or inserting the coils in grooves on the surface of the solenoid, or drawing vias that penetrate shells of the solenoid, forming a closed conductive loop around a microfluidic channel,, 'at least one microfluidic solenoid that measures electrical signals induced by a change of magnetic permeability from a target cell population where the target cell population has been magnetized, the at least one microfluidic solenoid'}a source of current to the solenoid, such that a sample containing a plurality of magnetized target cells flowing through the via in the solenoid produces a series of discrete changes in voltage induced by changes in permeability resulting from the magnetized target cells flowing therethrough,computer readable instructions that, when executed, causes quantitation of the number of discrete voltage changes indicative of the number of target cells in the target cell population.2. The device of further comprising interconnectively stacking a plurality of the microfluidic solenoids to provide parallel processing and high throughput.3. The device of having both horizontal arrays claim 1 , vertical arrays claim 1 , or both horizontal arrays and vertical arrays of microfluidic channels on a single device.4. A method of quantitating target cells in a sample claim 1 , the method comprisingusing a microelectromechanical system (MEMS) to fabricate one or more microfluidic solenoids, the one or more ...

SYSTEMS AND METHODS FOR COUNTING PARTICLES

Systems and methods are provided for counting particles in a fluid flow. In an aspect, coordinates of particles are obtained from video data of particles in a fluid, the video data made up of a sequence of image frames. The particle positions are linked in each pair of consecutive image frames of the video data. The linked particle positions are used to calculate particle trajectories through sequential image frames of the video data, and the particles are counted based on the particle trajectory. In another aspect, the particle positions within each image frame are transformed to estimated positions within a common coordinate frame. The estimated particle positions of a particle are grouped into a cluster center, and the particle count is calculated based on the cluster centers. 1. A method , comprising:obtaining a set of (x, y) coordinates corresponding to particle centers in video data of a set of particles within a fluid, the video data including a sequence of image frames and each set of (x, y) coordinates corresponds to an image frame of the sequence of image frames;linking a coordinate of each particle center in each pair of consecutive image frames of the sequence of image frames by fitting the coordinates to a statistical model of particle displacements;tracking, for each particle of the set of particles, a trajectory of that particle through links in each sequential image frame until that particle is not linked in a next image frame of the sequence of image frames;calculating, for each particle of the set of particles, a length of the tracked particle trajectory for that particle; andcalculating particle count based on the tracked particle trajectory of each particle of the set of particles.2. The method of claim 1 , further comprising capturing video data of the set particles claim 1 , the video data comprising the sequence of image frames take over time.3. The method of claim 2 , wherein the video data is captured using a lens-free imager.4. The method ...

A METHOD OF PREPARING A MILK SAMPLE, AND A DEVICE CONFIGURED TO BE USED WHEN PREPARING A MILK SAMPLE

A method for and a device to be used when preparing a milk sample that contains a first constituent and a second constituent. The device includes a first section (), a second section (), a third section () and fourth section (). The milk sample is centrifuged to obtain a plurality of sample parts including a first sample part of a first volume, in which first sample part the first constituent is contained, and a second sample part of a second volume, in which second sample part the second constituent is contained. A solution is provided and supplied to the device. At least one of the sample parts is removed from the milk sample. A remaining sample part and the solution are mixed to form a sample mixture in which the remaining sample part is distributed. The sample mixture is discharged from the device. 120-. (canceled)21. A method for preparing a milk sample , the method comprising the steps of:{'b': 1', '2', '3', '4', '6', '1', '11', '1', '2', '21', '2', '22', '2', '3', '31', '3', '32', '3', '4', '41', '42', '43', '4', '2', '3', '1', '2', '3', '4', '6', '1', '2', '3', '4', '6, 'providing a milk sample, containing at least a first constituent and a second constituent, to a device comprising a plurality of sections (, , , , ) that include at least i) a first section () forming a first communication passage () extending through the first section (), ii) a second section () forming a first fill chamber (), defining a first volume and extending through the second section (), and a first mixing chamber (), defining a volume equal to the first volume and extending through the second section (), iii) a third section () forming a second fill chamber (), defining a second volume and extending through the third section (), and a second mixing chamber (), defining a volume equal to the second volume and extending through the third section (), and iv) a fourth section () forming a second communication passage (, ; ) extending through the fourth section (), wherein at least the ...

AUTOMATED PLATELET FUNCTION ANALYZER AND ITS ANALYTICAL METHODS

An automated platelet function analyzer includes a sampling vessel, a preparation vessel, an analysis vessel, a sampling needle, a blood sample syringe, an analysis solution syringe, and a blood mixing device. A method for platelet analysis is also disclosed. 17-. (canceled)8. An analysis method of an automated platelet function analyzer having a sampling vessel , a preparation vessel , an analysis vessel , a sampling needle , a blood sample syringe , an analysis solution syringe , a blood mixing device , and a sample needle cleaner , the method comprising the following steps:Step 1: connecting the sampling needle with the blood sample syringe, using the sampling needle and the blood sample syringe to draw a whole blood sample from an associated test tube, moving the sampling needle into the sampling vessel to inject the whole blood sample into the sampling vessel for storing the whole blood sample in the sampling vessel, wherein the sampling vessel is placed in a sample holder, wherein the blood sample is mixed and stored in the sampling vessel;Step 2: using the sampling needle and the blood sample syringe to absorb a partial blood sample from the sampling vessel and transfer the partial blood sample to the preparation vessel;Step 3: using the analysis solution syringe to dispense analysis solution into the preparation vessel and mixing the partial blood sample to prepare a first blood sample dilution to generate a first diluted blood sample, wherein the analysis solution syringe is connected with an analysis solution storage container, the preparation vessel and the analysis vessel for dispensing analysis solution into the preparation vessel for preparing the first blood sample dilution and for dispensing analysis solution into the analysis vessel for preparing a second blood sample dilution, wherein the blood mixing device is an air pump connected with the sampling vessel, the preparation vessel, and the analysis vessel for mixing blood samples in the sampling ...

Bacterial counting method

Disclosed is a bacterial counting method, comprising the following steps, S1: preparing a polyaniline/bacterial composite film on the surface of a glassy carbon electrode by electric polymerization; S2: drawing the standard curve of the polyaniline/bacterial composite film modified electrode; and S3: determining the bacterial concentration of the bacteria solution sample to be tested according to the standard curve obtained in step S2. The method does not require the cultivation of the bacteria in the implementation process, such that same takes a short time and is easy to operate; the method uses aniline as the main reagent, and the consumption of the solution to be tested is small, such that the testing cost is low and the equipment is simple; the method has the advantages of a good repeatability and a wide detection linear range; and the method is an easy to operate, short time-consuming, and low cost bacterial counting method. The method is a bacterial counting method based on the polyaniline/bacterial composite film, and can achieve rapid and accurate detection of the bacterial thallus concentration.

GAS DETECTION SYSTEM FOR GYNECOLOGICAL DISEASE DETECTION AND DETECTION METHOD USING THE SAME

A gas detection system for gynecological disease detection and a detection method using the same are provided. The gas detection system is configured to detect an analyte from a female vagina and includes: a main body, a sleeve, a detection module, a pump, and a control module. The main body includes a body portion and a head portion having an intake channel. The body portion includes a detection chamber and an exhaust channel. The detection module includes at least one sensor configured to detect at least one target of the analyte and produce at least one detection signal. The pump is communicated with the detection chamber and the exhaust channel. The control module includes a processing unit and a first communication unit. The processing unit receives the at least one detection signal and controls the first communication unit to send the at least one detection signal. 1. A gas detection system for gynecological disease detection , configured to detect an analyte from a female vagina , including:a main body, including a body portion and a head portion protrudingly disposed on the body portion, the head portion having an intake channel disposed therethrough and configured to input the analyte, the body portion including a detection chamber communicated with the intake channel and an exhaust channel;a sleeve, detachably sleeved to the head portion, including a through slot corresponding to the intake channel;a detection module, including at least one sensor disposed in the detection chamber, the at least one sensor configured to detect at least one target of the analyte and produce at least one detection signal;a pump, disposed on the main body and communicated respectively with the detection chamber and the exhaust channel; anda control module, controlling operation of the detection module and the pump, including a processing unit and a first communication unit communicated with the processing unit, the processing unit receiving the at least one detection signal ...

SYSTEMS AND METHODS FOR COUNTING PARTICLES

Systems and methods are provided for counting particles in a fluid flow. In an aspect, coordinates of particles are obtained from video data of particles in a fluid, the video data made up of a sequence of image frames. The particle positions are linked in each pair of consecutive image frames of the video data. The linked particle positions are used to calculate particle trajectories through sequential image frames of the video data, and the particles are counted based on the particle trajectory. In another aspect, the particle positons within each image frame are transformed to estimated positions within a common coordinate frame. The estimated particle positions of a particle are grouped into a cluster center, and the particle count is calculated based on the cluster centers. 1. A method , comprising:obtaining a set of (x,y) coordinates corresponding to particle centers in video data of a set of particles within a fluid, the video data comprising a sequence of image frames and each set of (x,y) coordinates corresponds to an image frame;transforming each particle center of each image frame to an expected particle center in a common coordinate frame;grouping the transformed particle centers into cluster centers; andcalculating a particle count based on the number of cluster centers.2. The method of claim 1 , wherein transforming each particle center comprises computing a transform to describe particle displacement between consecutive image frames.3. A system claim 1 , comprising:a flow cell through including a fluid passage; capture video data of particles within the fluid using the image sensor, the video data comprising a sequence of image frames;', 'obtain a set of (x,y) coordinates corresponding to particle centers in the video data, each set of (x,y) coordinates corresponding to an image frame of the sequence of image frames;', 'transform each particle center of each image frame to an expected particle center in a common coordinate frame;', 'group the ...

HIGH EFFICIENCY MICROFLUIDIC DEVICE FOR TRAPPING CIRCULATING TUMOR CELLS

A microfluidic device for trapping circulating tumor cells includes at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel having a height of less than 50 μm and a width less than 30 μm. A plurality of expansion regions are disposed along the length of the at least one microfluidic channel, each of the plurality of expansion regions is formed by an abrupt increase in the width of the at least one microfluidic channel, wherein the width of each expansion region is within the range of 526 μm to 626 μm and continues for a length within the range of 814 μm to 914 μm along a length of the expansion region, followed by an abrupt decrease in the width of the at least one microfluidic channel back to a width less than 30 μm. 1. A microfluidic device for trapping cells comprising:at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel having a height of less than 50 μm and a width less than 30 μm; anda plurality of expansion regions disposed along a length of the at least one microfluidic channel, each of the plurality of expansion regions comprising an abrupt increase in the width of the at least one microfluidic channel, wherein the width of each expansion region is within the range of 526 μm to 626 μm and continues for a length within the range of 814 μm to 914 μm along a length of the expansion region, followed by an abrupt decrease in the width of the at least one microfluidic channel back to a width less than 30 μm.2. The microfluidic device of claim 1 , further comprising a pump connected to the inlet.3. The microfluidic device of claim 1 , wherein the at least one microfluidic channel comprises a plurality of microfluidic channels coupled to the inlet and the outlet.4. The microfluidic device of claim 1 , wherein the height of the at least one microfluidic channel is about 44 μm.5. The microfluidic device of claim 1 , wherein the width of the at least one ...

METHODS FOR DETECTING EVENTS IN A FLOW CYTOMETER

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided. 166-. (canceled)67. A flow cytometer , comprising:a flow cell;an optical particle interrogation system optically coupled to the flow cell; and extract putative event features from particles flowing through the flow cell;', 'time-stamp putative events;', 'determine a time difference between a putative previous event and a putative current event;', if the time difference is greater than the threshold duration, store the putative current event as a current event; and', if the peak height feature of the putative current event is less than the threshold peak height, discard the putative current event; and', 'if the peak height feature of the putative previous event is greater than the threshold peak height, store the putative previous event as a previous event., 'if the time difference is less than the threshold duration, compare a peak height feature of the putative current event and a peak height feature of the putative previous event to a threshold peak height, wherein], 'compare the time difference to a threshold duration, wherein], 'a non-transitory computer readable medium storing instructions that, when executed by a computing device, cause the computing device to68. The flow cytometer of claim 67 , wherein an event is distinguished from a signal selected from a group consisting of: optical system side-lobes claim 67 , fluidics drift claim 67 , baseline drift claim 67 , flow cell contamination claim 67 , and combinations thereof.69. The flow cytometer of claim 67 , wherein the particles are cells and an event is a cell event.70. The flow cytometer of claim 69 , wherein the cell event is a ...

METHODS FOR PREDICTING OVERALL SURVIVAL OF CANCER PATIENTS BASED ON NUMBERS OF CIRCULATING TUMOR CELLS

Cytokeratin (CK)+/CD45−/DAPI+ cells in the blood of cancer patients have been considered by most as circulating tumor cells (CTCs). Different methods of isolating CTCs results in a wide range of numbers and subgroups of CTCs from same patient. As a result, the clinical significance of the number of CTCs becomes cloudy. Provided herein is methodology to categorize CTCs into morphologically distinct subpopulations, and to use one of the subpopulations in methods for predicting overall survival of a patient having cancer. 1. A method for predicting overall survival of a patient having cancer , comprising enumerating pathologically-defined circulating tumor cells (PDCTCs) in a blood sample from the patient , wherein when five or more PDCTCs are present in 7.5 ml of blood , the overall survival of the patient is predicted to be lower than a patient having cancer with four or less PDCTCs present in 7.5 ml of blood , and wherein the PDCTCs are cytokeratin (CK) 8 , 18 , 19 , CD45 , DAPI cells , possess a malignant nucleus , and display a filamentous CK pattern.2. The method of claim 1 , wherein the cancer is breast cancer.3. The method of claim 1 , wherein the PDCTCs are enumerated by (i) isolating CTCs from the blood sample using a filter having pores of 7-8 microns in diameter and (ii) counting PDCTCs present in the isolated population of CTCs. Many recent publications reporting the presence of hundreds, to thousands, of circulating tumor cells (CTCs) in the blood of cancer patients have raised questions regarding the prevalence of CTCs, as enumerated by the CellSearch® Test. Although CellSearch® detects clinically relevant CTCs, the ability to capture only EpCAM+CTCs has led to speculation that CellSearch® captures only limited subsets of CTCs. In contrast, alternative isolation approaches often capture large numbers of CTCs from similar patient blood samples and, not surprisingly, these alternative approaches have poor correlations to CellSearch®. Given these problems, ...

Cell processing techniques

The present disclosure relates to cell processing techniques. By way of example, a cell processing system may include a plurality of sample processing devices configured to process patient samples and a plurality of readers respectively associated with the plurality of sample processing devices, wherein each reader is configured to read information from tracking devices associated with respective patient samples. The system may also include a controller that uses information from the readers to provide an estimated completion time for a patient sample based on availability of the sample processing devices.

System And Method For Fetal And Maternal Red Blood Cell Counting

A system for counting fetal and maternal red blood cells (RBCs) including a microscope and image capturing device to capture at least one image from a slide holding the fetal and maternal RBCs; a computer readable medium for storing the at least one image; a processor for executing computer readable instructions stored on a computer readable medium; wherein the computer executable instructions include instructions for: indentifying red blood cells from the at least one image; distinguishing between fetal and maternal red blood cells; and counting the fetal and maternal red blood cells. 1. A system for counting fetal and maternal red blood cells (RBCs) comprisinga microscope and image capturing device to capture a plurality of images from a slide holding the fetal and maternal RBCs; wherein said microscope is an X-Y stage microscope configured to centre said slide such that said plurality of images are captured by following a predetermined path around the centre of said slide;a computer readable medium for storing said at least one image;a processor for executing computer readable instructions stored on a computer readable medium;wherein said computer executable instructions include instructions for:identifying RBCs from said at least one image;distinguishing between fetal and maternal RBCs; andcounting said fetal and maternal RBCs.2. The system according to claim 1 , wherein said microscope includes an X-axis linear motion system and a Y-axis linear motion system.3. The system according to claim 2 , wherein said microscope is in communication with a controller including a driver for each of said X and Y axis linear motion systems.4. The system according to claim 3 , wherein said X-axis linear motion system and said Y-axis linear motion system are independently controllable.5. The system according to claim 1 , wherein said predetermined path consists of an S-shaped path.6. The system according to claim 6 , wherein said plurality of images comprises approximately 120 ...

METHOD OF OPERATING A PARTICLE SENSOR AND EVALUATION OF THE RESULTS THEREOF

The invention concerns a method of operating a particle sensor and evaluation of a result. A method of operating a particle sensor and evaluation of the results thereof comprises at least the following method steps: determining the frequency of the particle occurrences by means of the particle sensor; and signalling the exceeding of a predefinable threshold value, determined from the maximum particle number detected per time interval. 1. A method for operating a particle sensor together with the evaluation of its results , comprising at least the following method steps:Determining the frequency of particles events by means of the particle sensor andSignaling the exceeding of a predefinable limit value, determined from the maximum of the detected number of particles per time interval.2. The method according to claim 1 , characterized in that several time intervals (A claim 1 , B claim 1 , C) are defined in a sequence of increasing time and that each time interval (A claim 1 , B claim 1 , C) is assigned a predefined maximum number of particles (n claim 1 , n claim 1 , n).3. The method according to claim 1 , characterized in that a first time interval (A) is predefined in the range of minutes (min) claim 1 , a second time interval (B) in the range of hours (24 h) claim 1 , and a third time interval (C) in the range of days (d).4. The method according to claim 1 , characterized in that claim 1 , in the event of exceeding the respective predefinable limit value (n claim 1 , n claim 1 , n) and each time interval (A claim 1 , B claim 1 , C) claim 1 , an individual alarm signal claim 1 , identifiable as such (alarm A claim 1 , B claim 1 , C) claim 1 , is output.5. The method according to claim 1 , characterized in that both the length of the time intervals (A claim 1 , B claim 1 , C) claim 1 , as well as the maximum number of particles to be detected claim 1 , are predefinable by the user of the method.6. The method according to claim 1 , characterized in that the particle ...

Blood analysis method, control device, and blood cell analyzer

The present disclosure provided a blood cell analyzer, a control device and a blood analysis method thereof. In the method, a first reagent is mixed with a sample to obtain a first testing sample, and then a second reagent is mixed with the first testing sample for a further reaction to get a second testing sample for basophil classification and/or HGB measurement. A blood sample may be tested in one reaction cell through time-division multiplexing technology to obtain four groups leukocytes classification result and HGB result by single detection channel. Thus, the structure of the analyzer may be greatly simplified on the premise of guaranteeing the performance of the analyzer, the size and cost of the analyzer may reduce and a performance-price ratio of the analyzer may increase.

DEVICE FOR HOME MONITORING OF HAEMATOLOGICAL PARAMETERS OF PATIENTS

The present invention relates to a device for home monitoring of haematological parameters of patients. In particular, the device may be configured for home monitoring of patients undergoing chemotherapy. A white blood cell counter is presented in combination with a physically separated user device. The user interface and the white blood cell counter are configured to exchange data with each other. The present invention allows the white blood cell counter to be produced at lower costs, since no display is integrated into it directly. The patients may use the user interface device anywhere in their home or even on the go, allowing the maximum in freedom to fill in patient-reported outcomes or health assessment questionnaires. Also taking temperature readings is facilitated. In the home, a white blood cell test can be performed if required. The user interface guides the patient through the correct process and may therefore be brought to the cell counter. 3. Device according to claim 2 ,wherein the thermometer comprises an interface, in particular a Bluetooth interface, for wireless communication with the user interface.4. Device according to claim 1 ,wherein the white blood cell counter and the user interface are configured to provide the determined value of white blood cells in the received blood sample probe to the user interface.5. Device according to claim 2 ,wherein the user interface stores a white blood cell intervention level,wherein the user interface stores a temperature intervention level,wherein the user interface is configured for comparing the provided, determined value of the body temperature with the temperature intervention level, andwherein the user interface is configured for comparing the determined value of white blood cells in the received blood sample probe with the white blood cell intervention level.6. Device according to claim 5 ,wherein the user interface is configured for generating a signal based on a result of the comparison, andwherein ...

PORTAL AND METHOD FOR MANAGEMENT OF DIALYSIS THERAPY

System and method for point-of-care monitoring of neutrophils in a peritoneal dialysis sample with the use of a microfluidic system. The immunoassay based chip is configured to bound neutrophils to a microfluidic channel surface while leaving auxiliary cells and particles unattached and suspended in the sample and flushable with a wash buffer. Data representing images of neutrophils, formed by an (optionally lensless) imaging system, are processed to determine a count of neutrophils based on statistical parameters including characteristics of the microfluidic channel. 1. A system for at least one of identifying and counting target cells in a bodily fluid sample , the system comprising:an element with a network of microfluidic channels,an optical detector positioned adjacently to the element to receive light through the element, and acquiring data representing an initial image, of at least one channel from the network of microfluidic channels, formed on a surface of the optical detector in light that has traversed the at least one channel;', 'converting the acquired data to data representing a gray-scale image of said at least one channel;', 'data-processing of so-converted data by filtering these data in at least one of a frequency domain and a spatial domain; and', 'when said at least one channel of said network of microfluidic channels contains the bodily fluid sample with identified biological cells, identifying and optionally counting the target cells in relation to parameters of said at least one channel and a predefined threshold value., 'a non-transitory computer-readable medium having computer readable program code disposed therein, for at least one of identifying and counting the target cells in the bodily fluid sample, and comprising a series of computer-readable program steps to effect2. A system according to devoid of an optical component between the element and the optical detector.3. A system according to claim 1 , further comprising a display in ...

DEVICES AND METHODS FOR FRACTIONATED PHOTOACOUSTIC FLOW CYTOMETRY

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams. 1. A fractionated photoacoustic flow cytometry and theranostics system for in vivo detection , therapy , and monitoring of responses to the therapy for at least one target object in a biofluid system of a living organism , comprising:a laser system comprising more than one picosecond pulsed laser operable to provide more than one time-color encoded pulse of laser energy;a fractionated optical system configured to separate each laser beam into a plurality of laser beam fragments having a spatial configuration on the skin above the biofluid system of the living organism;at least one focused ultrasound transducer;a recording system operable for recording a combination of photoacoustic signals received by the at least one focused ultrasound transducer and emitted by the at least one target object in response to the more than one pulse of laser energy; anda triggering system operable to control the more than one pulsed lasers, synchronization of laser pulses from the more than one pulsed lasers with at least one time delay, and the recording system.2. The system of claim ...

CALIBRATED PARTICLE ANALYSIS APPARATUS AND METHOD

Calibrated particle analysis apparatus and method are provided. In the calibrated particle analysis apparatus, a gas exchange device and several flow controllers are disposed in front of a particle analyzer. Therefore, when the calibrated particle analysis apparatus is used, gases of a sample can be exchanged with a carrier gas suggested to be used with the particle analyzer. Hence, the accuracy of analyzing the particles can be increased, and possible hazards from dangerous or toxic materials can be avoided. 1. A calibrated particle analysis apparatus , comprising: an inner pipe having an inner gas inlet disposed at the first end, an inner gas outlet disposed at the second end, and a porous partition wall made from a porous material having properties of resistant to corrosion, non-reactive and not easily adhered to particles; and', 'an outer pipe sleeving outside the inner pipe and having an outer gas inlet adjacent to the second end, an outer gas outlet adjacent to the first end, and an outer pipe wall made from a corrosion resistant material;, 'a gas exchange device having a first end and a second end, the gas exchange device comprisinga first flow controller coupled to the inner gas inlet;a second flow controller coupled to the outer gas inlet;a third flow controller coupled to the outer gas outlet; anda particle analyzer coupled to the inner gas outlet for introducing particles in a sample carried by a carrier gas.2. The apparatus of claim 1 , wherein the inner pipe has an inner diameter of 1 to 30 mm and a length of 10 to 300 cm.3. The apparatus of claim 1 , wherein the first claim 1 , second claim 1 , and third flow controllers respectively comprise a mass flow controller claim 1 , a rotameter claim 1 , an orifice flow meter claim 1 , an ultrasonic flowmeter claim 1 , a flow-limiting valve claim 1 , or a needle valve.4. The apparatus of claim 1 , wherein the particle analyzer comprises a scanning mobility particle sizer (SMPS) claim 1 , a condensation ...

PARTICLE SENSOR

A particle mass concentration in an aerosol volume can be detected by means of an optical particle sensor (). In order to ensure that different degrees of contamination of the optical particle sensor () can be detected by means of said sensor and can be taken into consideration, the optical particle sensor () has a means () for identifying individual particles at low particle concentrations, for example up to 1000 particles/cm. 1110. A particle sensor , by means of which a particle mass concentration in an aerosol volume can be detected , characterized in that the particle sensor () has a means () for identifying individual particles at low particle concentrations , for example up to 1000 particles/cm.2210102102. The particle sensor according to claim 1 , which is configured as an aerosol photometer () and is equipped with an optical particle counter () as means for identifying individual particles claim 1 , wherein claim 1 , in a valid measurement range of the optical particle counter () claim 1 , a measured value thereof can be compared with that of the aerosol photometer () claim 1 , and a change in the ratio between the measured value of the optical particle counter () and the measured value of the aerosol photometer () can be recalculated into a degree of contamination of the particle sensor.31018. The particle sensor according to claim 1 , which is configured as an optical particle counter () and has a means () for detecting the static signal component.418161019. The particle sensor according to claim 3 , in which the means for detecting the static signal component is a DC voltage-coupled amplifier () claim 3 , the input of which is connected to a photodetector () of the optical particle counter () and the output of which can be scanned and processed by software by means of an A/D converter ().518. The particle sensor according to claim 4 , in which the DC voltage-coupled amplifier () has a very small temperature drift. The invention relates to a particle ...

REDUCING FALSE COUNTS IN CONDENSATION PARTICLE COUNTERS

Various embodiments include methods and apparatuses to reduce false-particle counts in a water-based condensation particle counter (CPC). In one embodiment, a cleanroom CPC has three parallel growth tube assemblies. A detector is coupled to an outlet of each of the three parallel growth tube assemblies, and is used to compare the particle concentrations measured from each of the three growth tube assemblies. An algorithm compares the counts from the three detectors and determines when the particles counted are real and when they are false counts. Any real particle event shows up in all three detectors, while false counts will only be detected by one detector. Statistics are used to determine at which particle count levels the measured counts are considered to be real versus false. Other methods and apparatuses are disclosed. 1. A system to determine false-particle counts in a condensation particle counter (CPC) , the system comprising:at least one aerosol sample inlet configured to be coupled to a sample flow pump;an aerosol flow path in fluid communication with and disposed between the aerosol sample inlet and the sample flow pump:a plurality of wicks each comprising a porous media forming at least a portion of the aerosol flow path; anda plurality of optical particle detectors each coupled to a respective outlet of one of the plurality of wicks to measure counts within the respective one of the plurality of wicks.2. The system of claim 1 , further comprising a processor coupled to each optical particle detector to compare a count from one of the plurality of wicks with a count of remaining ones of the plurality of wicks to make a determination whether the count from the one of the plurality of wicks is statistically greater than the counts of the remaining ones of the plurality of wicks claim 1 , the statistically greater count being considered to he a false count.3. The system of further comprising claim 2 , based on the determination that the count is ...

SYSTEM AND METHOD FOR ASSESSING QUANITITES OR SIZES OF LIPOPROTEIN PARTICLES FROM LIPOPROTEIN PARTICLE COMPOSITIONS

This invention relates to methods for assessing quantities of spherical or substantially spherical lipoprotein particles or portions thereof present in a biological sample based on the measurement of free cholesterol and/or phospholipid content in the lipoprotein particles. The invention also relates to the use of the assessed quantities of the lipoprotein particles or portions thereof to determine whether a subject is at increased risk for cardiovascular diseases and cardiodiabetes. 1. A method for assessing the quantities of one or more classes or subclasses of spherical or substantially spherical lipoprotein particles present in a biological sample , comprising:isolating one or more classes or subclasses of lipoprotein particles from the non-lipoprotein components in the biological sample, wherein the lipoprotein particles are spherical or substantially spherical;separating at least one of free cholesterol and phospholipid from the isolated spherical or substantially spherical lipoprotein particles;measuring the amount of the at least one of free cholesterol and phospholipid that has been separated from the isolated spherical or substantially spherical lipoprotein particles; anddetermining the quantities of the one or more classes or subclasses of spherical or substantially spherical lipoprotein particles based on the measured amount of the at least one of free cholesterol and phospholipid.2. The method of claim 1 , wherein the quantities of a plurality of different classes or subclasses of spherical or substantially spherical lipoprotein particles in the biological sample are accurately determined.3. The method of claim 1 , further comprising claim 1 , prior to the separating step:isolating the lipoprotein particles into two or more classes and/or subclasses, wherein the subsequent separating step is performed on one or more lipoprotein particles in one or more of the isolated classes and/or subclasses.4. The method of claim 1 , wherein either or both of the ...

METHOD OF TREATING PATIENTS WITH HEPATORENAL SYNDROME TYPE 1

The principles and embodiments of the present disclosure relate to methods for using terlipressin to treat a patient having impaired renal function associated with liver disease. A method of treating an adult patient with type 1 hepatorenal syndrome (HRS-1) may include assessing a baseline serum creatinine level prior to administration of terlipressin to the patient, initiating dosing of about 0.5 mg to about 1 mg of terlipressin to the patient every 6 hours by IV for 1-3 days, assessing a serum creatinine level in the patient at day 4±1 day from initiating dosing, administering a modified dosage of terlipressin based on a comparison of the assessed serum creatinine level at day 4±1 day and the baseline serum creatinine level, and continuing administration until 24 hours after two consecutive serum creatinine levels of ≤1.5 mg/dL at least 2 hours apart for a maximum of 14 days. The treatment may result in verified reversal of the HRS-1. 1. A method of reversing type 1 hepatorenal syndrome (HRS-1) , the method comprising:administering, to a patient having HRS-1, about 0.5 mg to about 1 mg of terlipressin every 6 hours for up to 3 days;measuring serum creatinine in the patient after 3 days administration; and if serum creatinine decreased by at least 30%, continue administering about 0.5 mg to about 1 mg terlipressin every 6 hours;', 'if serum creatinine has not decreased by 30%, administering about 1 mg to about 2 mg of terlipressin every 6 hours; and', 'if serum creatinine is at or above the baseline serum creatinine level, discontinue administering terlipressin., 'comparing the measured serum creatinine to a baseline serum creatinine level, wherein2. The method of claim 1 , wherein the terlipressin administered is terlipressin acetate.3. The method of further comprising continuing administration of terlipressin until 24 hours after two consecutive measured serum creatinine levels of ≤1.5 mg/dl at least 2 hours apart.4. The method of claim 3 , wherein administration ...

Method of inspecting liquid chemicals

In a method of inspecting liquid chemicals discharged from an ink jet head according to example embodiments, at least two laser beams may be irradiated onto the liquid chemicals discharged from the ink jet head, and the conditions of the liquid chemicals may be identified by detecting an interference pattern obtained from a laser scattering generated by passing the liquid chemicals through the at least two laser beams.

Station and method for measuring particle contamination of a transport carrier for conveying and storing semiconductor substrates at atmospheric pressure

The present invention relates to a station for measuring particle contamination of a transport carrier for conveying and storing semiconductor substrates at atmospheric pressure, said transport carrier comprising a rigid casing ( 2 ) containing an aperture and a removable door ( 3 ) allowing the aperture to be closed, the measuring station comprising: a controlled environment chamber ( 4 ) comprising at least one load port ( 8 ) capable of coupling, on the one hand, to the rigid casing ( 2 ), and on the other hand, to the door ( 3 ) of the transport carrier, in order to move the door ( 3 ) into the controlled environment chamber ( 4 ) and bring the interior of the rigid casing ( 2 ) into communication with the interior of the controlled environment chamber ( 4 ); and a measuring module ( 5 ) comprising a particle measuring unit ( 14 ) and a casing-measuring interface ( 16 ) configured to couple to the rigid transport carrier casing ( 2 ) coupled to the controlled environment chamber ( 4 ) in the place of the door ( 3 ), characterized in that said casing-measuring interface ( 16 ) comprises a measuring head protruding from a base of the casing-measuring interface, bearing a first sampling orifice ( 12 ) connected to the particle measuring unit ( 14 ), and at least two injecting nozzles ( 20 ) configured to direct a gas jet onto at least two separate locations on the rigid casing ( 2 ) coupled to the controlled environment chamber ( 4 ), the respective orientations of the injecting nozzles ( 20 ) being fixed relative to the coupled rigid casing ( 2 ). The invention also relates to a method for measuring particle contamination of a transport carrier for conveying and storing semiconductor substrates at atmospheric pressure

INTEGRATED AND STANDALONE LABEL AND REAGENT-FREE MICROFLUIDIC DEVICES AND MICROSYSTEMS FOR DIFFERENTIAL WHITE BLOOD CELL COUNTS

A method of establishing a differential white blood cell count includes directing at least one stream of deionized water into a microfluidic device containing a sample of whole blood or a cell-rich fraction to generate a lysate stream of intact white blood cells; directing at least one stream of deionized water into the lysate stream to form a virtual non-conductive aperture in a channel of the device; and performing impedance cytometry of the lysate stream via coplanar electrodes to detect the presence of intact white blood cells. A microfluidic device includes a blood separation section. An analyte sensor detects electrical changes in a cell-free fraction. Lysate from a cell-rich fraction is analyzed to detect circulating tumor cells or white blood cells including neutrophils, lymphocytes, monocytes, eosinophils, and basophils. A method of fabricating and a standalone cell-rich microfluidic device are disclosed for differential white blood cell counts. 1. A method of establishing a differential white blood cell count comprising:directing at least one stream of deionized water into a microfluidic device containing a sample of whole blood of a subject or a cell-rich fraction of a whole blood sample or a cell-free fraction of whole blood of a subject or combinations thereof to generate a lysate stream of intact white blood cells;directing at least one stream of deionized water into the lysate stream such that the lysate stream with intact white blood cells is forced to flow in a direction of motion by the at least one stream of deionized water to form a virtual non-conductive aperture in a channel of the microfluidic device; andperforming impedance cytometry of the lysate stream in the virtual non-conductive aperture via coplanar electrodes to detect the presence of intact white blood cells in the lysate stream.2. The method according to claim 1 , further comprisingquantitatively differentiating between neutrophils, lymphocytes, monocytes, eosinophils, and basophils ...

TREATMENT OF CANCERS USING PI3 KINASE ISOFORM MODULATORS

Provided herein are methods, kits, and pharmaceutical compositions that include a PI3 kinase inhibitor for treating cancers or hematologic disorders. 1. A method for treating a specific cancer or hematologic malignancy having a high expression level of one or more isoform(s) of phosphoinositide 3-kinase (PI3K) in a subject , comprising administering a PI3K modulator that selectively reduces the activity of said one or more isoform(s) of PI3K over other isoforms of PI3K.2. The method of claim 1 , wherein the method comprises the steps of: (1) determining the expression level of one or more PI3K isoform(s) in the cancer or hematologic malignancy; (2) selecting a PI3K modulator having a particular selectivity profile toward one or more PI3K isoform(s); and (3) administering the PI3K modulator to a patient having the cancer or disease claim 1 , alone or in combination with one or more other agents or therapeutic modalities.3. A method for treating a specific patient or group of patients claim 1 , having a cancer or hematologic malignancy claim 1 , wherein the patient(s) has(ve) a high expression level of one or more isoform(s) of PI3K claim 1 , comprising administering a PI3K modulator that selectively reduces the activity of said one or more isoform(s) of PI3K over other isoforms of PI3K.4. The method of claim 3 , wherein the method comprises the steps of: (1) determining the expression level of one or more PI3K isoform(s) in the patient or group of patients having the cancer or hematologic malignancy; (2) selecting a PI3K modulator having a particular selectivity profile toward one or more PI3K isoform(s); and (3) administering the PI3K modulator to the patient(s) claim 3 , alone or in combination with one or more other agents or therapeutic modalities.5. A method of determining whether a subject having a cancer or hematologic malignancy is more or less likely to respond to a treatment with a PI3K modulator that selectively reduces the activity of one or more isoform( ...

Devices for Detecting or Filtering Tumor Cells

Among others, the present invention provides apparatus comprising two micro-devices each fabricated by the method comprising: the first step of depositing a first material onto a substrate; the second step of depositing a second material onto the first material and then patterning the second material with a microelectronic technology or process; and repeating the second step at least once with a material that can be the same as or different from the first or second material. The micro-devices can pierce through the membrane of a circulating tumor cell and can move in different direction. 1. An apparatus comprising two micro-devices each fabricated by the method comprising:the first step of depositing a first material onto a substrate;the second step of depositing a second material onto the first material and then patterning the second material with a microelectronic technology or process; andrepeating the second step at least once with a material that can be the same as or different from the first or second material,wherein the micro-devices can pierce through the membrane of a circulating tumor cell and can move in different directions.2. The apparatus of claim 1 , wherein the micro-devices can measure at least a mechanical property claim 1 , an electrical property claim 1 , or an electro-magnetic property of the circulating tumor cell or cell membrane.3. The apparatus of claim 2 , wherein the electric property comprises surface charge claim 2 , surface potential claim 2 , resting potential claim 2 , electrical current claim 2 , electrical field distribution claim 2 , surface charge distribution claim 2 , cell electronic properties claim 2 , cell surface electronic properties claim 2 , electrical conductivity claim 2 , resistivity or resistance claim 2 , dynamic changes in electronic properties claim 2 , dynamic changes in cell electronic properties claim 2 , dynamic changes in cell surface electronic properties claim 2 , dynamic changes in surface electronic ...

APPARATUS FOR DETECTING TUMOR CELLS

Among others, the present invention provides apparatus for interacting with a biological subject to detect circulating tumor cells therein, comprising one device for sending a signal to the biological subject and optionally receiving a response to the signal from the biological entity. 1. An apparatus for interacting with a biological subject to detect circulating tumor cells therein , comprising one device configured for sending a signal to the biological subject and optionally receiving a response to the signal from the biological entity.2. The apparatus of claim 1 , wherein the interaction with the biological subject is probing claim 1 , detecting claim 1 , analyzing claim 1 , sorting claim 1 , communicating with claim 1 , treating claim 1 , correcting claim 1 , or modifying with an electrical claim 1 , magnetic claim 1 , electromagnetic claim 1 , thermal claim 1 , optical claim 1 , acoustical claim 1 , biological claim 1 , chemical claim 1 , electro-mechanical claim 1 , electro-chemical claim 1 , electro-optical claim 1 , electro-thermal claim 1 , electro-chemical-mechanical claim 1 , bio-chemical claim 1 , bio-mechanical claim 1 , bio-optical claim 1 , bio-thermal claim 1 , bio-physical claim 1 , bio-electro-mechanical claim 1 , bio-electro-chemical claim 1 , bio-electro-optical claim 1 , bio-electro-thermal claim 1 , bio-mechanical-optical claim 1 , bio-mechanical thermal claim 1 , bio-thermal-optical claim 1 , bio-electro-chemical-optical claim 1 , bio-electro-mechanical-optical claim 1 , bio-electro-thermal-optical claim 1 , bio-electro-chemical-mechanical claim 1 , physical or mechanical property claim 1 , or a combination thereof claim 1 , of the biologic subject.3. The apparatus of claim 1 , wherein the device comprises multiple surfaces coated with one or more elements or combinations of elements claim 1 , and a control system configured for releasing the elements.4. The apparatus of claim 1 , wherein the device comprises a first component configured for ...

PARTICLE COUNTING APPARATUS

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level. 2. The particle counting apparatus according to claim 1 , whereinthe plurality of counting chambers are cylindrically-shaped containers with a same inner diameter, anda liquid level of the sample liquid in a counting chamber in which the counting is completed last is higher than the liquid level of the sample liquid in the one or more predetermined counting chambers at the time point when the sample liquid has been injected into each of the plurality of counting chambers.3. The particle counting apparatus according to claim 1 , whereinthe plurality of counting chambers comprise a red blood cell counting chamber and a white blood cell counting chamber,a counting chamber in which the counting is completed last is the red blood cell counting chamber, andthe one or more predetermined counting chambers are white blood cell counting chambers.4. The particle counting apparatus according to claim 1 , whereinthe liquid supplied by the liquid supplying part is a diluent, andthe liquid supplying part comprises one or both of a diluent supplying device of the following (I) and a sampling-nozzle washer of the following (II):(I) a diluent supplying device comprising a diluent supplying source, a diluent supply port provided in the one or more predetermined counting chambers, and a diluent supplying piping connecting the diluent supplying source and the diluent supply port(II) a sampling nozzle washer comprising a diluent supplying source, a diluent outlet part accompanying a sampling nozzle provided in the particle counting ...

SYSTEMS AND METHODS FOR DIAGNOSING A FLUIDICS SYSTEM AND DETERMINING DATA PROCESSING SETTINGS FOR A FLOW CYTOMETER

The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. Systems and methods for determining data processing settings require an accurate measurement of peak times among various channels and being able to adjust time delay settings wherein peak time is the measurement of time elapsed from the beginning of the data collection time window to the highest peak in the window. 131.-. (canceled)32. A fluidic diagnostic system for a flow cytometer comprising:a flow cell configured to flow calibration particles;at least two light sources each configured to emit a light beam, wherein each light beam is associated with a channel, and wherein the light beams pass through the flow cell;a detector associated with each channel, wherein each detector is configured to collect light emitted from each of the set of calibration beads;a memory buffer configured to record data from each of the detectors;a trigger channel configured to initiate a transfer of data from a first data collection time window associated with the trigger channel when a data signal threshold for the trigger channel is exceeded;a second channel configured to transfer data from a second data collection time window associated with the second channel when the data signal threshold for the trigger channel is exceeded; transfer the data from the first data collection time window to a data storage each time the data signal intensity threshold is exceeded; and', 'transfer the data from the second data collection time window to the data storage each time the data signal intensity threshold is exceeded; and, 'a trigger processor configured toa computer processor configured to compare a distribution of data intensity peak times within the second data collection time window to ...

Microfluidic interrogation device

A portable, stand-alone microfluidic interrogation device including a microprocessor and a touch-screen display. The touch-screen display can receive one or more user input to select a particular particle interrogation procedure, and subsequently show interrogation results. A microfluidic path extending through the interrogation device includes alignment structure that defines an interrogation zone in which particles carried in a fluid are urged toward single-file travel. Operable alignment structure may define sheath-, or non-sheath fluid flow. Desirably, a portion of the alignment structure is removable from the device in a tool-free procedure. The device may operate under the Coulter principle, and/or detect Stokes' shift phenomena, and/or other optically-based signal(s).

SYSTEM AND METHOD FOR ASSESSING QUANTITIES OR SIZES OF LIPOPROTEIN PARTICLES FROM LIPOPROTEIN PARTICLE COMPOSITIONS

This application discloses methods for assessing quantities of spherical or substantially spherical lipoprotein particles or portions thereof present in a biological sample based on the measurement of free cholesterol and/or phospholipid content in the lipoprotein particles. Methods of treating a subject at increased risk for cardiovascular disease and/or cardiodiabetes are also disclosed. 1. A method of treating a subject at increased risk for cardiovascular disease and/or cardiodiabetes , the method comprising: isolating one or more classes or subclasses of lipoprotein particles from the non-lipoprotein components in the biological sample, wherein the lipoprotein particles are spherical or substantially spherical;', 'separating at least one of free cholesterol and phospholipid from the isolated spherical or substantially spherical lipoprotein particles;', 'measuring the amount of the at least one of free cholesterol and phospholipid that has been separated from the isolated spherical or substantially spherical lipoprotein particles; and', 'determining the quantities or the average sizes of one or more classes or subclasses of spherical or substantially spherical lipoprotein particles based on the measured amount of the at least one of free cholesterol and phospholipid;, 'a) assessisng the quantities or the average sizes of one or more classes or subclasses of spherical or substantially spherical lipoprotein particles present in a biological sample, by a method comprisingb) comparing the determined quantities or average sizes of the one or more classes or subclasses of spherical or substantially spherical lipoprotein particles to a control or reference value to determine if the subject is at an increased risk for cardiovascular disease and/or cardiodiabetes; andc) administering a drug or a supplement to the subject.2. The method of claim 11 , wherein the quantities of a plurality of different classes or subclasses of spherical or substantially spherical lipoprotein ...

METHOD OF TREATING PATIENTS WITH HEPATORENAL SYNDROME TYPE 1

The principles and embodiments of the present disclosure relate to methods for using terlipressin to treat a patient having impaired renal function associated with liver disease. A patient identified as suffering from HRS-1 is tested to determine if the patient meets at least two out of three criteria, wherein the three criteria include a WBC<4 or >12 cells/4; HR>90 bpm; and any one of HCO3<21 mmol/L or PaCO2<32 mmHg or >20 breaths per minute. If the patient meets at least two of the criteria, he or she is administered terlipressin in an amount effective to produce a reduction in serum creatinine of at least 1.0 mg/dL.

SYSTEMS FOR SINGLE OR MULTIPLE CELL COUNTING AND DISPENSING

The present invention provides methods, device, assemblies, and systems for counting and dispensing single or multiple cells (e.g., into the open wells of a multi-well testing device). In certain embodiments, the systems comprise: a) a fluid movement component composed of upstream and downstream electrode conduits connected to a non-conductive conduit, and b) an electronic signal detector electrically linked to the upstream and downstream electrode conduits such that, when a fluid is present in the fluid movement component, an electrical circuit is established that is altered when a cell passes through the non-conductive conduit. In other embodiments, the systems comprises a) a fluid movement component composed of an upstream electrode conduit connected to a non-conductive conduit, b) an in-well electrode, and c) an electronic signal detector electrically linked to the upstream electrode conduit and the in-well electrode. 1. A system comprising: i) an upstream electrode conduit comprising a proximal end, a distal end, and an upstream fluid-carrying channel, wherein said upstream electrode conduit is electrically conductive and able to transmit said cell in said fluid therethrough;', 'ii) a downstream electrode conduit comprising a proximal end, a distal end, and a downstream fluid-carrying channel, wherein said downstream electrode conduit is electrically conductive and able to transmit said cell in said fluid therethrough; and', 'iii) a non-conductive conduit comprising a proximal end, a distal end, and a non-conductive fluid-carrying channel, wherein said non-conductive conduit is non-electrically conductive and able to transmit said cell in said fluid therethrough,', 'wherein said proximal end of said non-conductive conduit is connected to said distal end of said upstream electrode conduit, and said distal end of said non-conductive conduit is connected to said proximal end of said downstream electrode conduit; and, 'a) a fluid movement component configured to ...

Reducing false counts in condensation particle counters

Various embodiments include methods and apparatuses to reduce false-particle counts in a water-based condensation particle counter (CPC). In one embodiment, a cleanroom CPC has three parallel growth tube assemblies. A detector is coupled to an outlet of each of the three parallel growth tube assemblies, and is used to compare the particle concentrations measured from each of the three growth tube assemblies. An algorithm compares the counts from the three detectors and determines when the particles counted are real and when they are false counts. Any real particle event shows up in all three detectors, while false counts will only be detected by one detector. Statistics are used to determine at which particle count levels the measured counts are considered to be real versus false. Other methods and apparatuses are disclosed.

MODULAR PACKAGING SYSTEM FOR A LUBRICANT CONDITION MONITOR

An apparatus for assessment of a fluid system includes a scaffold housing with a plurality of internal cavities; a debris monitor module assembly to be selectively inserted into a first cavity of the plurality of internal cavities, the debris monitor module assembly to determine wear debris information in a lubricant; a lubricant condition monitor module assembly to be selectively inserted into a second cavity of the plurality of internal cavities, the lubricant condition monitor module assembly to determine lubricant condition information in the lubricant; and a processing module assembly that is configured to be selectively inserted into a third cavity of the plurality of internal cavities, the processing module assembly to provide communication to an external interface of at least one of the wear debris information and the lubricant condition information. 1. An apparatus for assessment of a fluid system , comprising:a scaffold housing with a plurality of internal cavities;a debris monitor module assembly to be selectively inserted into a first cavity of the plurality of internal cavities, the debris monitor module assembly to determine wear debris information in a lubricant;a lubricant condition monitor module assembly to be selectively inserted into a second cavity of the plurality of internal cavities, the lubricant condition monitor module assembly to determine lubricant condition information in the lubricant; anda processing module assembly that is configured to be selectively inserted into a third cavity of the plurality of internal cavities, the processing module assembly to provide communication to an external interface of at least one of the wear debris information and the lubricant condition information.2. The apparatus of claim 1 , further comprising an electrical connector to receive signals from each of the debris monitor module assembly claim 1 , the lubricant condition monitor module assembly claim 1 , and the processing module assembly.3. The ...

Particle Manipulation System with Out-of-plane Channel and Recovery Algorithm

A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has an inlet channel, output channels, and a movable member formed on a substrate. The movable member moves parallel to the fabrication plane, as does fluid flowing in the inlet channel. The movable member separates a target particle from the rest of the particles, diverting it into an output channel. However, at least one output channel is not parallel to the fabrication plane. The device may be used to separate a target particle from non-target material in a sample stream. In the event that the microfabricated particle manipulation device malfunctions as a result of a particle of debris becoming lodged in the microfabricated particle manipulation device, the system may invoke a recovery algorithm, that includes vibrating the microfabricated particle manipulation device using a pulse train at a frequency near its mechanical resonance. 1. A particle manipulation system comprising:a microfabricated particle sorting device,a detecting device that generates a signal indicating that a malfunction has occurred; anda controller that invokes a recovery algorithm upon receiving the signal, wherein the recovery algorithm includes generating a plurality of pulses that vibrate the particle sorting device.2. The particle manipulation system of claim 1 , wherein the plurality of pulses comprises at least one of a series of square waves claim 1 , sinusoids claim 1 , trapezoid and ramps.3. The particle manipulation system of claim 1 , wherein at least one second pulse follows within about 50 microseconds an initial pulse.4. The particle manipulation system of claim 1 , wherein the plurality of pulses comprises a train of pulses with a range of frequencies.5. The particle manipulation system of claim 1 , wherein the range of frequencies includes a mechanical resonance frequency of the microfabricated particle sorting device.6. The particle manipulation system of claim 4 , wherein ...

Methods and Apparatus for Real-Time Detection and Clearing of a Clog

A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well. 1. A method , comprising:providing a flow cytometer system comprising (a) a flow cell having a first end and a second end, (b) a sample fluidic pathway having a first end and a second end, wherein the second end of the sample fluidic pathway is coupled to the first end of the flow cell, (c) a sample probe coupled to the first end of the sample fluidic pathway, (d) a sample pump in fluid communication with the sample fluidic pathway, (e) a waste line having a first end and a second end, wherein the first end of the waste line is coupled to the flow cell, and (f) a waste pump in fluid communication with the waste line;activating the waste pump to apply negative pressure to one or more of the waste line, the flow cell, and the sample fluidic pathway;activating the sample pump; andcycling the sample probe into and out of a decontamination solution reservoir and driving a decontamination fluid, via the sample pump, through one or more of the flow cell, the sample fluidic pathway and the waste line, thereby clearing a clog.2. The method of claim 1 , further comprising:cycling the sample probe into and out of a cleaning solution reservoir; ...

SOLVENT COMPOUND FOR A PARTICLE COUNTER/IMAGER AND ASSOCIATED METHOD

A solvent compound for a particle counter/imager system, the solvent compound includes a liquid solvent miscible with oil to remove oil from the particle counter/imager system and a liquid dispersive surfactant configured to break large water droplets into smaller droplets less than a predetermined size. The liquid dispersive surfactant is miscible with the solvent and is nontoxic and nonflammable. 1. A solvent compound for a particle counter/imager system , the solvent compound comprising:a liquid solvent miscible with oil to remove oil from the particle counter/imager system; anda liquid dispersive surfactant configured to break large water droplets into smaller droplets less than a predetermined size, the liquid dispersive surfactant miscible with the solvent, nontoxic, and nonflammable.2. The solvent compound of in which a volume of the liquid solvent in the compound is in the range of about 16% to about 99% and a volume of the liquid dispersive surfactant in the compound is in the range of about 1% to about 15%.3. The solvent compound of in which the volume of the liquid solvent in the compound is about 97.5% and the volume of the liquid dispersive surfactant in the compound is about 2.5%.4. The solvent compound of in which the liquid solvent comprises hydrotreated isoparaffins and naphthenics.5. The solvent compound of in which the liquid dispersive surfactant comprises Octylphenol Ethoxlate.6. The solvent compound of in which the liquid dispersive surfactant is configured to break the large water droplets having a size greater than about 20 microns into smaller particles having a size less than about 20 microns.7. The solvent compound of in which the liquid dispersive surfactant is configured to break large water droplets having a size greater than about 20 microns into smaller droplets having a size less than about 4 microns.8. A method of testing an oil sample claim 6 , the method comprising:urging a first oil sample through an imaging subsystem to image ...

Magnetic flow cytometry

Die Erfindung betrifft die magnetische Durchflusszytometrie. Zur Vermeidung von Probenkontamination, sowie zur Vermeidung von Kontakt mit infektiösen Zellproben wird eine Vorrichtung angegeben, die ein geschlossenes System für die Zellmessung darstellt. Dabei umfasst das geschlossene Durchflusssystem ein Probenreservoir, einen Durchflusskanal sowie eine Ansaugeinrichtung. Insbesondere ist das geschlossene System modular und separat von einer Mess- und Auswertestation ausgeführt. Auf die Messstation können mehrere derartiger Durchflusssysteme aufgesetzt werden. Infektiöses Zellmaterial ist mitsamt der Vorrichtung entsorgbar. The invention relates to magnetic flow cytometry. To avoid sample contamination and to avoid contact with infectious cell samples, a device is specified which represents a closed system for cell measurement. The closed flow system includes a sample reservoir, a flow channel and a suction device. In particular, the closed system is modular and designed separately from a measuring and evaluation station. Several such flow systems can be placed on the measuring station. Infectious cell material can be disposed of together with the device.

Target cell detection method

Counting particles using an electrical differential counter

Sorting and counting of biological particles, comprises marking with paramagnetic or dielectric nanoparticles, to be passed a sensor which registers field changes to give signals which can be counted electronically

Sorting and counting biological particles, where the non-agglutinate particles are marked with paramagnetic or dielectric nano particles of 5-500 nm. The marking is through bi-functional molecules, genetic probes, viruses or specific adsorbent proteins, and the marked biological particles are boosted in an electrical or magnetic field before or after counting. Sorting and counting biological particles, where the non-agglutinate particles are marked with paramagnetic or dielectric nano particles of 5-500 nm. The marking is through bi-functional molecules, genetic probes, viruses or specific adsorbent proteins, and the marked biological particles are boosted in an electrical or magnetic field before or after counting. The biological particles are carried by a shrouding liquid continuously or separately as a string of pearls past a sensor (46), which can be capacitative or magnetic e.g. gradiometeric, magnetoresistive or flux-gate. The sensor is a component part of a micro-electromechanical system (40) with micro-fluid elements of 5-500 mum. The field changes registered as the marked particles flow past are converted into signals which can be counted electronically.

Cartridge for a magnetic flow cytometer, magnetic flow cytometer and method for analyzing a sample with such a cartridge

Die Erfindung bezieht sich auf eine Kartusche (1) für ein magnetisches Durchflusszytometer, die sich hauptsächlich in einer xy-Ebene erstreckt, mit einem Einlass (2) zum Injizieren einer Probe (15) in die Kartusche (1), einem Blister (3) für eine Pufferlösung (21) mit magnetischen Markern zum Markieren von vorgegebenen Teilchen (16, 16') der Probe (15), einem Auslass und einem Fluidkanal (9), wobei der Fluidkanal (9) einen ersten Teil, der den Einlass (2) mit dem Blister (3) verbindet, und einen zweiten Teil, der den ersten Teil mit dem Auslass verbindet, aufweist, wobei der zweite Teil des Fluidkanals (9) eine Anreicherungszone (5) mit mechanischen Führungsstrukturen zum Fokussieren der markierten Teilchen (16, 16') der Probe (15) in einem vorbestimmten Teilabschnitt des Fluidkanals (9) und eine Messzone (6) zwischen der Anreicherungszone (5) und dem Auslass aufweist, wobei die Messzone (6) einen Magnetfeldsensor (14) in dem vorbestimmten Teilabschnitt des Fluidkanals (9) aufweist, um vereinfachte und schnellere Mittel zum Messen von Teilchen, insbesondere Konzentrationen von Teilchen, einer Probe bereitzustellen. The invention relates to a cartridge (1) for a magnetic flow cytometer, which extends mainly in an xy plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3). for a buffer solution (21) with magnetic markers for marking predetermined particles (16, 16 ') of the sample (15), an outlet and a fluid channel (9), wherein the fluid channel (9) has a first part connecting the inlet (2 ) and a second part connecting the first part to the outlet, the second part of the fluid channel (9) having an enrichment zone (5) with mechanical guiding structures for focusing the marked particles (16, 16). 16 ') of the sample (15) in a predetermined section of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, wherein the measuring zone (6) comprises a magnetic field sensor (14) in ...

Apparatus for registering and classifying biological particles bonded to magnetic beads, comprises a sensor coil separate from the magnetic field generator, for reduced interference in the signals

Apparatus to register and classify particles in a suspension, which can be magnetized and especially biological particles or molecules, generates an alternating magnetic field for the suspension fluid to pass through with the magnetized particles. Apparatus to register and classify particles in a suspension, which can be magnetized and especially biological particles or molecules, generates an alternating magnetic field for the suspension fluid to pass through with the magnetized particles. The oscillating magnetic field is set at an excitation frequency. A sensor, with a separate coil (1) assembly from the magnetic field generator, detects the magnetic condition of the particles to register those with higher magnetic oscillation signals and especially higher than the excitation of those triggered by the magnetic field by a whole number multiple. The sensor is linked to an electronic evaluation unit to classify the detected particles from the reaction to the detector signals.

Method and apparatus for magnetic flow cytometry

A magnetic flow cytometry apparatus (1) for detection of cells labeled with magnetic nanoparticles comprising at least one pair of oppositely oriented magnets (2) to provide between said magnets a first magnetic field region (3) with a low magnetic field strength and to provide at poles of said magnets second magnetic field regions (4, 5) with a high magnetic field strength, wherein the magnetic labeled cells (8) provided within a flow input into said magnetic flow cytometry apparatus (1) are enriched in at least one of the second magnetic field regions (4) and supplied to the first magnetic field region (3), where a magnetic field is applied to the enriched magnetic labeled cells (8) to measure the magnetic relaxation of the magnetic labeled cells (8) in response to the applied magnetic field.

Particulate matter sensor device

Fine particle counter and fine particle counter chip

Cartridge for a magnetic flow cytometer, a magnetic flow cytometer, and method for analysing a sample with such a cartridge

The invention relates to a cartridge (1) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet (2) for injecting a sample (15) into the cartridge (1), a blister (3) for a buffer solution (21) with magnetic markers to mark pregiven particles (16, 16') of the sample (15), an outlet, and a fluid channel (9), the fluid channel (9) comprising a first part that connects the inlet (2) with the blister (3) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel (9) comprises an enrichment zone (5) with mechanical guiding structures to focus marked particles (16, 16') of the sample (15) in a predetermined subsection of the fluid channel (9) and a measuring zone (6) between the enrichment zone (5) and the outlet, the measuring zone (6) comprising a magnetic field sensor (14) in the predetermined subsection of the fluid channel (9)in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.

Method of Characterizing Particles

Mixtures containing homogeneously-sized particles with a minimum concentration of agglomerates or larger particles are desired in various manufacturing processes such as, for example, in the manufacture and use of chemical mechanical polishing slurries, food emulsions, pharmaceutical products, paints, and print toner. The method disclosed herein provides these industries with an accurate and efficient method of screening such mixtures for such agglomerates and large particles. The method generally includes preparing a suspension of the mixture in an electrolyte, wherein the suspension includes a specified concentration of small particles per unit of electrolyte. The method further includes passing the prepared suspension, and a plurality of the particles therein, through an aperture of a device capable of characterizing particles according to the Coulter principle to obtain data on the particles. Still further, the method includes deriving a particle size distribution of the large particles from the obtained data. The suspension includes at least one small particle per aperture volume. The large particles have an average diameter that is at least five times greater than the average diameter of the small particles. The aperture has a diameter that is (i) at least 50 times greater than the average diameter of the small particles, and (ii) about 1.2 times greater than the average diameter of the large particles to less than about 50 times greater than the average diameter of the large particles.

PARTICLE COUNTING DEVICE

Un dispositif de comptage de particules comprend un détecteur agencé pour produire un signal de mesure électrique en réponse au passage d'une ou plusieurs particules, et un comparateur agencé pour comparer le signal de mesure avec un signal seuil et pour incrémenter une valeur de comptage lorsque le signal de mesure dépasse le signal seuil, caractérisé en ce qu'il comprend en outre un circuit d'ajustement de seuil qui applique un filtre passe-bas au signal de mesure, et qui est relié au comparateur pour utiliser le signal résultant comme signal seuil. A particle counting device includes a detector arranged to produce an electrical measurement signal in response to the passage of one or more particles, and a comparator arranged to compare the measurement signal with a threshold signal and to increment a count value when the measurement signal exceeds the threshold signal, characterized in that it further comprises a threshold adjustment circuit which applies a low-pass filter to the measurement signal, and which is connected to the comparator to use the resulting signal as signal threshold.

Particle count correction method and apparatus

A method is disclosed which allows more accurate counting of particles where the sample has significant size variability between particles. The method uses flight time and wait time to obtain a corrected count of particles.

Systems and methods for particle counting

Systems and methods consistent with embodiments of the present invention provide a method for the measurement and analysis of particle counts in flow cytometry and hematology instruments. In some methods for the measurement and analysis of particle counts, a corrected histogram of particle distributions is calculated and used to obtain an accurate count of particles and an accurate measurement of other particle parameters.

Method and apparatus for magnetic flow cytometry

A magnetic flow cytometry apparatus (1) for detection of cells labeled with magnetic nanoparticles comprising at least one pair of oppositely oriented magnets (2) to provide between said magnets a first magnetic field region (3) with a low magnetic field strength and to provide at poles of said magnets second magnetic field regions (4, 5) with a high magnetic field strength, wherein the magnetic labeled cells (8) provided within a flow input into said magnetic flow cytometry apparatus (1) are enriched in at least one of the second magnetic field regions (4) and supplied to the first magnetic field region (3), where a magnetic field is applied to the enriched magnetic labeled cells (8) to measure the magnetic relaxation of the magnetic labeled cells (8) in response to the applied magnetic field.

Solvent compound for a particle counter/imager and associated method

A solvent compound for a particle counter/imager system, the solvent compound includes a liquid solvent miscible with oil to remove oil from the particle counter/imager system and a liquid dispersive surfactant configured to break large water droplets into smaller droplets less than a predetermined size. The liquid dispersive surfactant is miscible with the solvent and is nontoxic and nonflammable.

Method for detecting particles in a fluid stream

Method for testing a cell sample

In this invention, a cell parameter, for example cell volume, is determined by subjecting one or more aliquots of a sample cell suspension to one or more alterations of at least one parameter of the cell environment to identify a point at which the cells achieve a particular shape to obtain a sample specific shape compensation factor. Preferably, the environmental parameter change is a reduction in osmolality.

Device and process for detecting particles in a flowing liquid

A device for detecting electrically conductive particles in a liquid flowing in a pipe section, with a transmitter coil surrounding the pipe section for inducing eddy currents in the particles, at least one first inductive receiver coil surrounding the pipe section and a second inductive receiver coil which is spaced axially to the first receiver coil and which surrounds the pipe section, the first and the second receiver coils being located in the region of the transmitter coil and being subtractively connected, in order to output a difference signal according to the eddy currents induced by the transmitter coil, and the transmitter coil forming the primary side and the receiver coils forming the secondary side of a transformer arrangement. An evaluation unit evaluates the difference signal in order to detect passage of electrically conductive particles in the pipe section.

Method and device for measuring particles in a fluid stream

Die Erfindung betrifft eine Vorrichtung zum Erfassen von elektrisch leitenden Partikeln (20) in einer in einem Rohrstück (10) strömenden Flüssigkeit (16), mit einer das Rohrstück (10) umgebenden Senderspule (18) zum Induzieren von Wirbelströmen in den Partikeln (20), mindestens einer ersten das Rohrstück (10) umgebenden induktiven Empfängerspule (12) und einer zweiten das Rohrstück (10) umgebenden, axial zur ersten Empfängerspule (12) beabstandeten, induktiven Empfängerspule (14), wobei die erste (12) und die zweite Empfängerspule (14) im Bereich der Senderspule (18) angeordnet sind und subtraktiv geschaltet sind, um ein Differenzsignal entsprechend den von der Senderspule (18) induzierten Wirbelströmen auszugeben, und wobei die Senderspule (18) die Primärseite und die Empfängerspulen (12, 14) die Sekundärseite einer transformatorischen Anordnung bilden, sowie einer Auswerteeinheit (38) zum Auswerten des Differenzsignals, um den Durchgang von elektrisch leitenden Partikeln (20) in dem Rohrstück (10) zu erfassen. The invention relates to a device for detecting electrically conductive particles (20) in a liquid (16) flowing in a pipe section (10) with a transmitter coil (18) surrounding the pipe section (10) for inducing eddy currents in the particles (20). at least a first inductive receiver coil (12) surrounding the tubular member (10) and a second inductive receiver coil (14) surrounding the tubular member (10) axially spaced from the first receiver coil (12), the first (12) and second receiver coils (14) are arranged in the region of the transmitter coil (18) and are switched subtractive in order to output a difference signal corresponding to the eddy currents induced by the transmitter coil (18), and wherein the transmitter coil (18) the primary side and the receiver coils (12, 14) Form secondary side of a transformer arrangement, and an evaluation unit (38) for evaluating the difference signal to the passage of electrically conductive particles ln (20) in the ...

SENSOR DEVICES

Cartridge for a magnetic flow cytometer, a magnetic flow cytometer, and method for analysing a sample with such a cartridge

The invention relates to a cartridge ( 1 ) for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet ( 2 ) for injecting a sample ( 15 ) into the cartridge ( 1 ), a blister ( 3 ) for a buffer solution ( 21 ) with magnetic markers to mark pregiven particles ( 16, 16 ′) of the sample ( 15 ), an outlet, and a fluid channel ( 9 ), the fluid channel ( 9 ) comprising a first part that connects the inlet ( 2 ) with the blister ( 3 ) and a second part that connects the first part with the outlet, wherein the second part of the fluid channel ( 9 ) comprises an enrichment zone ( 5 ) with mechanical guiding structures to focus marked particles ( 16, 16 ′) of the sample ( 15 ) in a predetermined subsection of the fluid channel ( 9 ) and a measuring zone ( 6 ) between the enrichment zone ( 5 ) and the outlet, the measuring zone ( 6 ) comprising a magnetic field sensor ( 14 ) in the predetermined subsection of the fluid channel ( 9 ) in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.

Flow cytometry system with bubble detection

The flow cytometry system of the present invention includes a flow channel including an interrogation zone. A light source and a light detector are connected to the interrogation zone, such that a sample flowing through the interrogation zone can be optically analyzed through methods known in the art of flow cytometry. A bubble detector is connected to the flow channel. A controller is connected to the bubble detector and is adapted to perform a predetermined output in response to the detection of a bubble in the flow channel. The predetermined output may include alerting a user as to the presence of a bubble, flagging potentially corrupted data, and ceasing data collection until the interrogation zone is clear of bubbles.

System and method for particle counting

Automated analyzer and method for screening cells or formed bodies for enumeration of populations expressing selected characteristics

내용없음 No content

Analyzer, assay cartridge and analyzing method

Analyzers are disclosed that comprises an assay cartridge (1) comprising a mixture measuring chamber (7) for receiving a mixture of a sample and a dilution fluid, and a detector (9) for detecting a signal from the mixture supplied from the mixture measuring chamber (7), the mixture measuring chamber (7) having a predetermined capacity, and an amount of the mixture supplied to the detector (9) being substantially equal to the capacity of the mixture measuring chamber; and an analyzing unit comprising a controller for analyzing the signal detected by the detector; wherein the assay cartridge (1) is detachably mountable to the analyzing unit. An assay cartridges and analyzing method are also described.

Vertical suction type Beta-ray fine dust measuring device

본 발명은 기존의 베타레이를 이용한 미세먼지 측정 장치의 공기 유입부 구조를 수직형으로 개선하고, 베타레이를 이용한 미세먼지의 연속측정 방법을 제공하고자 하는 것이다. 이를 위하여 외부의 대기에서 공기를 유입하여, 상기 공기 중에 포함된 미세입자를 분리하여 미세먼지의 농도를 측정할 수 있도록 미세먼지를 분리하는 미세먼지분리부(200); 및 상기 미세먼지 분리부에서 분리된 측정대상인 미세먼지와 공기가 수직하게 하부로 흐를 수 있는 통로가 되는 수직공기샘플유입부(210); 및 상기 수직공기샘플유입부를 지나는 상기 미세먼지를 모두 걸러주는 롤타입여지(400); 및 상기 롤타입여지의 하단에 연결되어 상기 공기를 흡입하는 흡입부로 구성되며, 상기 롤타입여지 상기 걸러진 미세먼지를 측정하기 위하여 베타레이를 방출하는 베타레이발생부(300); 및 상기 베타레이발생부(300)에서 발생된 베타레이가 상기 롤타입여지에 걸러진 미세먼지를 투과하여 감소한 베타레이를 측정하는 베타레이측정부(310);를 포함하는 것을 특징으로 하는 수직흡입형 베타레이 미세먼지 측정장치를 제공한다. 본 발명의 상기와 같은 구성에 의하여 미세먼지가 공기통로에 흡착되거나, 눌러 붙지 않고, 롤타입여지에 모두 걸러져 미세먼지를 정확하게 측정할 수 있는 효과가 있다. An object of the present invention is to improve the structure of the air inlet of the existing fine dust measuring device using beta-ray to a vertical type, and to provide a method for continuous measurement of fine dust using beta-ray. To this end, a fine dust separation unit 200 that introduces air from the outside atmosphere, separates the fine particles contained in the air, and separates the fine dust so as to measure the concentration of the fine dust; and a vertical air sample inlet 210 that is a passage through which the fine dust and air, which are the measurement target separated by the fine dust separation unit, can flow vertically downward; And a roll type filter 400 that filters all the fine dust passing through the vertical air sample inlet; And it is connected to the lower end of the roll-type filter paper consists of a suction unit for sucking the air, the roll-type filter paper Beta-ray generator 300 for emitting beta-ray to measure the filtered fine dust; and a beta-ray measuring unit 310 for measuring the reduced beta-ray by penetrating the fine dust in which the beta-ray generated by the beta-ray generator 300 is filtered through the roll-type filter paper; Beta-ray fine dust measuring device is provided. According to the above configuration of the present invention, the fine dust is not adsorbed or pressed into the air ...

Fine dust measuring device using beta-ray and measuring method using the same

본 발명은 기존의 베타레이를 이용한 미세먼지 측정 장치의 공기 유입부의 구조를 개선하여 공기유입부 파이프에 미세 먼지가 침칙하는 것을 막고자 하는 것이다. 이를 위하여, 공기유입부(110)에서 유입된 공기는, 직관식공기유입파이프(125)를 통과하여 베타레이 발생부(200)와 베타레이 측정기(300)를 구비한 측정몸체의 측면에 사선방향으로 형성된 공기통로를 통하여 롤타입여지를 통과하면서 미세먼지가 상기 롤타입 여지에 걸러지고, 상기 베타레이 발생부에서 발생된 베타레이는 상기 미세먼지가 걸러진 여지를 통과하며, 상기 미세먼지의 양이 많아짐에 따라 상기 여지를 통과한 베타레이 신호의 세기가 감소하게 되는 것이어서, 상기 베타레이 측정기에서 측정된 베타레이 신호의 크기 변화로부터 미세먼지의 양을 측정하는 것을 특징으로 하는 베타레이를 이용한 미세먼지 측정 장치를 제공한다. 상기와 같은 구성에 의하여 공기유입파이프 중간에 미세 먼지가 쌓이지 않는 효과가 있어 정확한 미세먼지 측정이 가능하다.

Measuring method of immature granulocytes

Device for counting small particles and a sorting apparatus comprising such a device

A device for counting small particles, especially carriers, comprises a capillary (2) for receiving the particles to be counted, a light source (3) for emitting measuring light (M) and a photoelectric detector (5) for receiving the measuring light (M). Also provided are light-delivering means which deliver the measuring light (M) emitted by the light source (3) to the capillary (2) and light-removing means which deliver the measuring light (M) coming from the capillary (2) to the photoelectric detector (5). The internal diameter (ID) of the capillary (2) is such that the individual particles can enter the capillary (2) only one behind the other and can move along inside it only one behind the other. The light-delivering means irradiate the capillary (2) with the measuring light (M) transversely to the longitudinal axis (A) thereof, the measuring light (M) being in the form of a light beam, the width of which is such that only one of the particles can be completely in the light beam at any one time. A sorting apparatus for distributing small particles comprises such a device.

Particle sorting apparatus, particle sorting method, program, and particle sorting system

Provided are a particle sorting apparatus, particle analysis method, program, and particle sorting system capable of simply detecting air bubbles, foreign matter, and the like, in a droplet. A determination unit provided in a particle sorting apparatus determines whether photographed image information including photographed droplet image information pertaining to the brightness of an image of a droplet including particles that has been photographed after being discharged from an orifice has changed in relation to preset reference image information including reference droplet image information pertaining to the brightness of an image of a droplet that has been photographed after being discharged from an orifice.

Microfluidic apparatuses for fluid movement control

According to an example, a microfluidic apparatus may include a fluid slot and a foyer that is in fluid communication with the fluid slot via a channel having a relatively smaller width than the foyer. The microfluidic apparatus may also include an electrical sensor to measure a change in an electrical field caused by a particle of interest in a fluid passing through the channel from the fluid slot to the foyer, an actuator to apply pressure onto fluid contained in the foyer, and a controller to receive the measured change in the electrical field from the electrical sensor, determine, from the received change in the electrical field, an electrical signature of the particle of interest, and control the actuator to control movement of the particle of interest based upon the determined electrical signature of the particle of interest.

Particulate matter sensor device

미립자 센서 장치(1)는, 유동 입구(11)와 유동 출구(12) 및 이들 사이에 연장되어 있는 유동 채널(2)을 포함하는 인클로저(21); 에어로졸 샘플이 유동 채널(2)을 통해 안내될 때 그 에어로졸 샘플의 유동(20) 내에 있는 미립자와 방사선의 상호 작용을 위해 방사선을 유동 채널(2) 안으로 방출하기 위한 방사선원; 미립자와의 상호 작용 후에 방사선의 적어도 일부분을 검출하기 위한 방사선 검출기(4)를 포함한다. 센서 장치는, 방사선 검출기(4) 및/또는 방사선원(3) 상으로의 그리고/또는 방사선원(3) 및/또는 방사선 검출기(4)에 가까운 채널 벽 부분 상으로의 미립자 침전을 줄이도록 유동(20)을 변경시키기 위해 방사선 검출기(4) 및/또는 방사선원(3)의 상류에 배치되는 유동 변경 장치(511)를 포함한다. 본 발명은 또한 그러한 미립자 센서 장치를 사용하여 에어로졸 샘플 내의 미립자의 파라미터를 결정하는 방법에 관한 것이다.

Analysis method and analysis device

An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.

Blood cell counter, diagnosis support method and computer program product

A blood cell counter apparatus comprising: a detector for detecting blood cells in blood of a subject; and a controller for obtaining, based on a detection result by the detector, first analytical information about nucleated red blood cells in the blood, and second analytical information about granulocytes in the blood or third analytical information about platelets in the blood, and for outputting diagnosis support information for supporting prognosis of the subject, based on the first analytical information and on the second analytical information or the third analytical information that have been obtained. A method and computer program product are also disclosed.

METHOD AND DEVICE FOR ANALYZING A BLOOD SAMPLE

APPARATUS FOR COUNTING AND DETERMINING AT LEAST ONE LEUKOCYTAIC SUB-POPULATION.

Apparatus for counting and determining at least one leucocytic sub-population

An apparatus is disclosed for counting and determining at least one leucocytic sub-population, comprising a case (1) for injecting a flow of sample to be analyzed inside a measurement tank (5) through which a light beam (24) passes, comprising internal (16) and external (10) nozzles for sleeving the sample by means of a pressurized liquid. Electrodes for the resistivity measurements of the leucocytic sub-population are component elements (8, 16) of the injection case. Units for collecting and processing the signals delivered by the optical sensor and the electrodes are also provided.

METHOD AND SYSTEM FOR COUNTING CELLS ONLINE AND IN SITU IN A BIOLOGICAL CULTURE MEDIUM

Analysis method and analyzer

Microfluidic component used for electrical impedance measurement through a biological object

L’invention concerne un composant microfluidique (1) employé pour une mesure d'impédance électrique à travers un objet biologique, ledit composant comprenant : Un espace microfluidique (10) comprenant une zone dite de mesure (2),Au moins deux électrodes (40, 41) agencées en vis-à-vis, de part et d'autre de la zone de mesure (2),Le composant (1) étant formé par l'assemblage d'au moins deux couches superposées suivant un plan de jonction longitudinal, dites couche inférieure (3) et couche supérieure (5),Les deux couches présentant chacune au moins une cavité (30, 50),Les deux couches étant assemblées l'une sur l'autre de manière à placer les deux cavités en vis-à-vis pour former ledit espace microfluidique. Figure à publier avec l'abrégé : Figure 1 The invention relates to a microfluidic component (1) used for electrical impedance measurement through a biological object, said component comprising: A microfluidic space (10) comprising a so-called measurement zone (2),At least two electrodes (40 , 41) arranged facing each other, on either side of the measurement zone (2), the component (1) being formed by assembling at least two superposed layers along a longitudinal junction plane , called lower layer (3) and upper layer (5),The two layers each having at least one cavity (30, 50),The two layers being assembled one on the other so as to place the two cavities facing -à-vis to form said microfluidic space. Figure to be published with abstract: Figure 1

METHOD AND SYSTEM FOR COUNTING CELLS ONLINE AND IN SITU IN A BIOLOGICAL CULTURE MEDIUM

Procédé pour compter en ligne et in situ dans cellules dans un milieu de culture biologique, comprenant les étapes suivantes:-une pluralité de mesures de la capacitance du milieu ou une pluralité de mesures de la conductance de ce milieu, à des fréquences distinctes variant dans une plage de fréquences de mesure prédéterminée,- une extraction d'une information de variation de permittivité due à la beta-dispersion dans ce milieu, à partir des mesures de capacitance, et- un traitement de la variation de cette information de variation de permittivité en fonction de la fréquence, pour délivrer une information de comptage de cellules dans ledit milieu. A method for in-line and in-cell counting in cells in a biological culture medium, comprising the following steps: a plurality of measurements of the medium capacitance or a plurality of measurements of the conductance of this medium, at different frequencies varying in a predetermined measurement frequency range, - an extraction of a permittivity variation information due to the beta-dispersion in this medium, from the capacitance measurements, and a processing of the variation of this permittivity variation information as a function of frequency, for outputting cell count information in said medium.