10-03-2022 дата публикации
Номер: US20220074853A1
Принадлежит:
Qualitative and quantitative methods are for the detection of alginate oligomers in body fluids based on analyzing the Fourier transform infrared spectroscopy (FTIR) spectrum of a body fluid sample, for example a sputum sample, at a specific wave number range and, more particularly, certain specific characteristic wavenumbers. 1. A method for determining the concentration of alginate oligomer in a sample of body fluid , said method comprising{'claim-text': [{'sup': ['−1', '−1'], '#text': '(a) 1596 cmto 1606 cm,'}, {'sup': ['−1', '−1'], '#text': '(b) 1407 cmto 1417 cm,'}, {'sup': ['−1', '−1'], '#text': '(c) 1120 cmto 1130 cm,'}, {'sup': ['−1', '−1'], '#text': '(d) 1081 cmto 1091 cm,'}, {'sup': ['−1', '−1'], '#text': '(e) 1023 cmto 1033 cm, and'}, {'sup': ['−1', '−1'], '#text': '(f) 943 cmto 953 cm;'}], '#text': '(i) obtaining for the sample of body fluid one or more normalized IR absorbance values, one or more normalized IR transmittance values, one or more second derivative IR absorbance values, or one or more second derivative IR transmittance values, at one or more wavenumbers selected from one or more of the following wavenumber ranges:'}(ii) for one or more of the wavenumbers selected in step (i) providing corresponding IR absorbance values, IR transmittance values, second derivative IR absorbance values, or second derivative IR transmittance values, for a plurality of samples of said body fluid which contain a range of known amounts of the alginate oligomer, and(iii) determining the concentration of the alginate oligomer in the sample of body fluid by determining the relative position of the values obtained in step (i) amongst the values provided in step (ii).2. The method of claim 1 , wherein:{'sup': ['−1', '−1'], '#text': '(i) the wavenumber range of (a) is 1597 cmto 1605 cm,'}{'sup': ['−1', '−1'], '#text': '(ii) the wavenumber range of (b) is 1408 cmto 1416 cm,'}{'sup': ['−1', '−1'], '#text': '(iii) the wavenumber range of (c) is 1121 cmto 1129 cm,'}{'sup': ...
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