VERFAHREN ZUR KONTINUIERLICHEN TRENNUNG VON GEMISCHEN MIKROSKOPISCH KLEINER, DIELEKTRISCHER TEILCHEN UND VORRICHTUNG ZUR DURCHFÜHRUNG DES VERFAHRENS

VERFAHREN UND VORRICHTUNG ZUR ISOELEKTRISCHEN TEILCHENTRENNUNG

EXCHANGEABLE CARTRIDGE FOR THE CLEANING OF MACROMOLECULES

CRACKING OF MEANS DIELEKTROPHORESIS AND CRACKING WITH APPLICATION OF A RIVER FIELD

METHOD AND APPARATUS FOR FRACTIONATION USING CONVENTIONAL DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

FRACTIONATION USING DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. The invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which matter are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position.

MICROMACHINED ELECTRICAL FIELD-FLOW FRACTIONATION SYSTEM

L'invention porte sur un système micro-usiné pouvant effectuer un fractionnement en continu de petits échantillons de fluide d'essai. Le système inclut un dispositif à microcanaux (12) qui comprend un premier substrat (14) présentant une surface interne planaire sur laquelle est formée une électrode (21). Un deuxième substrat (16), qui présente une surface interne planaire comportant une électrode (22), est placé sur le premier substrat de sorte que ces électrodes respectives soient disposées en vis-à-vis. Une couche intermédiaire isolante (18) est interposée entre le premier et le deuxième substrats. Cette couche intermédiaire est modelée de manière à former des parois latétales opposées d'au moins un microcanal, les électrodes formées sur les substrats délimitant des bordures continues opposées le long du microcanal. Des orifices d'entrée et de sortie (24, 26) sont formés sur un seul substrat ou sur les deux pour permettre l'écoulement du fluide à l'intérieur ou hors du microcanal. Ce ...

System and method for programmable illumination pattern generation

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present ...

PROCESS FOR THE CONTINUOUS SEPARATION OF MIXTURES OF MICROSCOPICALLY SMALL DIELECTRIC PARTICLES AND DEVICE FOR IMPLEMENTING THE PROCESS

The description relates to a process for separating mixtures of microscopically small dielectric particles in suspension and a device for implementing the process. Prior art separation processes separate particle mixtures according to the differing mobilities of their components. This requires extensive measures to suppress convection in the suspension. In the process of the invention, the particle mixture is forced onto tracks by dielectrophoretic forces or by the flow of the suspension medium. An additional force which, for specific particle types, offsets the force driving the particles onto tracks, separates particles of these types out of the mixture. The device for implementing the process is suitable for system integration. It can be integrated on surfaces of silicon wafers economically and in large numbers. The device is suitable for isolating small particles like biological cells, cell organella, bio-molecules and organic dielectric particles.

DNA extraction microchip, system and method

PROCEDURE AND DEVICE FOR THE ISO-ELECTRICAL PARTICLE SEPARATION

FRACTIONATION USING DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

The present disclosure is directed to a novel apparatus and novel methods fo r the separation, characterization, and manipulation of matter. The invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which matter are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position.

ELECTROPHORETIC SEPARATION OF COMPOUNDS

A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

ELECTROPHORETIC SEPARTION OF COMPOUNDS

Methods and apparatus for nonlinear mobility electrophoresis separation

APPLICATION OF STATIC LIGHT TO A FLUID FLOW OF CNTs FOR PURPOSES OF SORTING THE CNTs

A method is described that comprises sorting carbon nanotubes (CNTs) within a fluidic flow for a targeted subset of CNTs. The sorting comprises attracting at least a portion of the CNTs within the fluidic flow in a direction of increasing intensity of an electric field component of a substantially stationary beam of light. The electric field component has a frequency that is less than one or more resonant frequencies of the CNTs within the portion.

Method for continuous separation of a mixture of fine dielectric particles and apparatus for carrying out the method

Vorrichtung zur Trennung von Gemischen mikroskopisch kleiner, in einer Flüssigkeit oder einem Gel suspendierter dielektrischer Teilchen

ELEKTROPHORETISCHE TRENNUNG VON VERBINDUNGEN

APPARATUSES AND METHODS FOR FIELD FLOW FRACTIONATION OF PARTICLES USING ACOUSTIC AND OTHER FORCES

This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of mattes utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separtion, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods ...

Application of static light to a fluid flow of CNTs for purposes of sorting the CNTs

System and method for programmable illumination pattern generation

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (“gray scales”) of ...

Electrical field-flow fractionation using redox couple added to carrier fluid

Improved apparatus and procedures for electrical field-flow fractionation is disclosed. A test sample is injected into the liquid carrier stream flowing through the flow channel of the apparatus. The apparatus comprises a thin flow channel having a top, a bottom, and two side walls, with a fluid carrier inlet at one end and an outlet at the other end. The top and bottom walls are formed such that at least the inner surfaces thereof that face the flow channel are made of an electrically conductive material so that the inner surfaces thereof form electrodes as well as opposite, broad boundary surfaces of the flow channel. A voltage differential is applied to the electrically conductive inner surfaces of the top and bottom walls. The carrier fluid flowing through the channel can be deionized or distilled water as well as water containing a red-ox couple such as quinone/hydroquinone.

FRACTIONATION USING DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. The invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which matter are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position.

Electrophoretic separation of compounds

LIQUID FLOWING TYPE CARRIER ELECTROPHORESIS APPARATUS

PURPOSE: To obtain a new separating apparatus, wherein high-speed liquid chromatography using a new column filler is adopted in an electrophoresis method, by using a granular material as a carrier, and flowing a liquid forcibly and steadily in the direction perpendicular to a DC electric field. CONSTITUTION: An elute 1 folws through a filler 3, which is held between a pair of parallel plates, through a liquid dispersing plate 13 by a pump 11. A sample is continuously sent through a pipe 12 by switching a valve 18. Ion exchanged films are closely contacted with both side surfaces of the parallel plates, so that the filler is not spilled out of the side surfaces. An electric field is imparted by electrodes 5 and 5' from the side at the same time. At the inlet port at the lower end of the flat shaped column, several tens of pipes having filters are arranged. The elute flows out trough the pipes. Theses pipes are guided to a multi-path photometer 15, which projects a monochromatic light beam ...

FRACTIONATION USING DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. The invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which matter are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position.

DNA EXTRACTION MICROCHIP, SYSTEM AND METHOD

A DNA extraction microchip, system for and method of extracting DNA from a sample including DNA-containing material, including: a main channel through which first and second parallel flows are in use delivered, the first flow being of a sample including DNA-containing material and the second flow being of a separation medium; and an electrode unit for applying a first, electroporation field to the first flow of sample to including DNA-containing material, the electroporation field being such as to effect electroporation of DNA-containing material, and a second, electroseparation field across the main channel, the electroseparation field being such as to effect electroseparation of DNA from the electroporated DNA-containing material in the first flow into the second, parallel flow.

Vorrichtung zur Trennung von Gemischen mikroskopisch kleiner, in einer Flüssigkeit oder einem Gel suspendierter, dielektrischer Teilchen

Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation

Fractionation using dielectrophoresis and field flow fractionation

Method of continuously separating mixtures of microscopic dielectric particles and apparatus for carrying through this method

A method of separating mixtures of microscopic dielectric particles in sunsions in an apparatus for carrying the method. A mixture of particles is forced onto guide paths by dielectrophoretic forces or by a flow of the suspension medium with an additional force, which is provided to compensate the force causing the particles to move along the guide paths for specific particle species causing the specific particle species to be fed out from the mixture of particles. The apparatus may be integrated on surfaces of silicon wafers at low cost and in mass-production numbers, and is suitable for isolating minute particles such as biological cells, cell organelles, bio molecules as well as organic dielectric particles.

Apparatus for separating mixtures of microscopic small dielectric particles dispersed in a fluid or a gel

Verfahren zur Trennung von Gemischen mikroskopisch kleiner, in einer Flüssigkeit oder einem Gel suspendierter dielektrischer Teilchen und Vorrichtung zur Durchführung des Verfahrens

Anwendung von statischem Licht auf einen fluidischen Strom von CNTs zum Zwecke des Sortierens der CNTs

Verfahren, das aufweist Sortieren von Kohlenstoff-Nanoröhrchen (CNTs) innerhalb einer fluidischen Strömung für eine ins Ziel gefaßte Untermenge der CNTs, wobei das Sortieren das Anziehen wenigstens eines Teiles der CNTs innerhalb der fluidischen Strömung in eine Richtung anwachsender Intensität einer elektrischen Feldkomponente von im wesent lichen stationärem Laserlicht aufweist, wobei die elektrische Feldkomponente eine Frequenz hat, die kleiner ist als eine oder mehrere Resonanzfrequenzen der CNTs in dem Teil ist.

Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation

Micromachined electrical field-flow fractionation system

ELECTROPHORETIC SEPARTION OF COMPOUNDS

A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

Compound & method

ELEKTROPHORETISCHE TRENNUNG VON VERBINDUNGEN

Cassette for macromolecule purification

DNA EXTRACTION MICROCHIP, SYSTEM AND METHOD

Application of static light to a fluid flow of cnts for purposes of sorting the cnts

描述了一种方法，包括拣选流体流中的多个碳纳米管(CNT)以得到多个CNT的目标子集。拣选包括沿着基本静态的光束的电场分量的强度增强的方向，吸引流体流中的多个CNT的至少一部分。电场分量的频率低于该部分中的多个CNT的一个或多个谐振频率。

APPARATUSES AND METHODS FOR FIELD FLOW FRACTIONATION OF PARTICLES USING ACOUSTIC AND OTHER FORCES

This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of mattes utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

Electrokinetic concentration of charged molecules

ELECTROKINETIC CONCENTRATION OF CHARGED MOLECULES

A method for separating and concentrating charged species from uncharged or neutral species regardless of size differential. The method uses reversible electric field induced retention (135 and 130) of charged species, that can include molecules and molecular aggregates such as dimers, polymers, multimers, colloids, micelles, and liposomes, in volumes and on surfaces of porous materials (120). The retained charged species are subsequently quantitatively removed from the porous material by a pressure driven flow that passes through the retention volume and is independent of direction, thus, a multi-directional flow field is not required. Uncharged species pass through the system unimpeded thus effecting a complete separation of charged and uncharged species and making possible concentration factors greater than 1000-fold.

MINIATURIZED ELECTROTHERMAL FLOW INDUCED INFUSION PUMP

A micropump that pumps liquid using electrothermally-induced flow is described, along with a corresponding self-regulating pump and infusion pump. The micropump has applications in microfluidic systems, such as biochips. The self-regulating infusion pump is useful for administration of large and small volumes of liquids such as drugs to patients and can be designed for a wide range of flow rates by combining multiple micropumps in one infusion pump system. The micropump uses electrode sequences on opposing surfaces of a flow chamber that are staggered with respect to each other. The opposing surfaces include staggered electrodes that have the same phase and same electrode sequence. As such electrodes with the same phase are staggered and not eclipsed.

Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods ...

Fractionation using dielectrophoresis and field flow fractionation

Electrophoretic separation of compounds

Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exists at the opposite end of the chamber at a characteristic position. Methods ...

APPARATUSES AND METHODS FOR FIELD FLOW FRACTIONATION OF PARTICLES USING ACOUSTIC AND OTHER FORCES

This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of mattes utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exists at the opposite end of the chamber at a characteristic position. Methods ...

Fractionation device for blood or other extracted substances, has magnets that generate magnetic field in direction perpendicular to mainstream direction of substance or blood plasma received in plastic or glass container

The device has a container (1) for receiving the substance e.g. blood plasma (8) for fractionation. A magnetic field generating mechanism is arranged to produce a magnetic field in a direction perpendicular to the mainstream direction of the blood plasma. The container may be made of plastics, glass or acrylic glass. The magnetic field generating mechanism includes magnets (6) arranged with opposing poles surrounding the container, and aligned symmetrical to the longitudinal axis of the container.

Electrode design for electrical field flow fractionation

An improved channel for separation of molecular and particulate samples by means of electrical field flow fractionation (FFF) is disclosed. The electrodes therein are made of metal oxide coated flat glass plates and permit the achievement, thereby, all of the seven most desirable features for such an electrical FFF channel, viz. 1) hardness, 2) flatness, 3) smoothness, 4) chemical inertness, 5) durability (does not generate particles), 6) high conductivity, and 7) transparency. In addition, the design of the clamping plates permits high precision in aligning the electrodes for parallelism.

Elektrodendesign für die elektrische Feldflussfraktionierung

Encoded random arrays matrices

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present ...

Verfahren und Vorrichtung zur isoelektrischen Teilchentrennung

In order to isoelectrically separate particles with a pH-dependent net charge, the particles are exposed in a guiding liquid to electric field forces. The pH value of the guiding liquid is set in such a way that at least one predetermined type of particle is separated from the remaining particles and migrates to a fixing collecting means under the effect of the electric field forces. The collecting means is for example a porous hollow fibre delimited by electrodes which generate the electric field forces and crossed by the guiding liquid together with the sample to be separated. The particles whose isoelectric point matches the pH value of the guiding liquid run unimpeded through the fibres, whereas the remaining particles are pressed against the inner wall of the fibre and are prevented from being carried away with the liquid flow.

Elektrodendesign für die elektrische Feldflussfraktionierung

APPLICATION OF STATIC LIGHT TO A FLUID FLOW OF CNTs FOR PURPOSES OF SORTING THE CNTs

A method is described that comprises sorting carbon nanotubes (CNTs) within a fluidic flow for a targeted subset of CNTs. The sorting comprises attracting at least a portion of the CNTs within the fluidic flow in a direction of increasing intensity of an electric field component of a substantially stationary beam of light. The electric field component has a frequency that is less than one or more resonant frequencies of the CNTs within the portion.

METHOD AND APPARATUS FOR FRACTIONATION USING CONVENTIONAL DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

Ｃｎｔを選別するためにｃｎｔの流体流に静的な光を当てる方法

従来の誘電泳動およびフィールドフロー分別法を使用する分別法のための方法および装置

... 本発明は、異なる型の粒子状物質および可溶化物質の識別のための方法および装置を提供する。この識別は、例えば、粒子状物質の分離、特徴付け、識別、および操作を包含し得る。本発明に従って、粒子状物質は、装置内に投入される前に、液体懸濁物中に配置され得る。識別は、装置内で生じ得、この装置は、薄い密封チャンバであり得る。粒子は、例えば、その密度、サイズ、誘電率、導電率、表面電荷、および/または表面構造における差異によって識別され得る。生物学的細胞の場合、これらは、そのサイズ、密度、膜の電気的なキャパシタンスおよびコンダクタンス、内部の導電率および誘電率、ならびに/または表面電荷における差異に従って識別され得る。 ...

Application of static light to a fluid flow of cnts for purposes of sorting the cnts

描述了一种方法，包括拣选流体流中的多个碳纳米管(CNT)以得到多个CNT的目标子集。拣选包括沿着基本静态的光束的电场分量的强度增强的方向，吸引流体流中的多个CNT的至少一部分。电场分量的频率低于该部分中的多个CNT的一个或多个谐振频率。

Verfahren zur kontinuierlichen Trennung von Gemischen mikroskopisch kleiner, dielektrischer Teilchen und Vorrichtung zur Durchführung des Verfahrens

The description relates to a process for separating mixtures of microscopically small dielectric particles in suspension and a device for implementing the process. Prior art separation processes separate particle mixtures according to the differing mobilities of their components. This requires extensive measures to suppress convection in the suspension. In the process of the invention, the particle mixture is forced onto tracks by dielectrophoretic forces or by the flow of the suspension medium. An additional force which, for specific particle types, offsets the force driving the particles onto tracks, separates particles of these types out of the mixture. The device for implementing the process is suitable for system integration. It can be integrated on surfaces of silicon wafers economically and in large numbers. The device is suitable for isolating small particles like biological cells, cell organella, bio-molecules and organic dielectric particles.

FRAKTIONIERUNG MITTELS DIELEKTROPHORESIS UND FRAKTIONIERUNG UNTER ANWENDUNG EINES FLUSSFELDES

FRAKTIONIERUNG MITTELS DIELEKTROPHORESIS UND FRAKTIONIERUNG UNTER ANWENDUNG EINES FLUSSFELDES

METHOD AND APPARATUS FOR FRACTIONATION USING CONVENTIONAL DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

L'invention concerne des procédés et des dispositifs permettant la discrimination de matière dans une chambre à orifice d'admission et orifice d'évacuation, par diélectrophorèse et fractionnement en continu. Un support vecteur est introduit dans l'orifice d'admission et dirigé depuis cet orifice d'admission vers l'orifice d'évacuation selon un profil de vitesse. Un signal de tension programmé est appliqué à une électrode couplée à la chambre, de manière à appliquer une force diélectrophorétique à la matière. Cette force est équilibrée au moyen d'une force de gravitation, ce qui permet de déplacer la matière pour lui donner des positions conformes au profil de vitesse sur le support vecteur, aux fins de discrimination de la matière. On peut utiliser une chambre à orifices d'évacuation supérieur et inférieur, afin d'extraire une première partie de support vecteur au niveau de l'orifice d'évacuation supérieur, selon un premier débit de liquide contrôlable, et afin d'extraire une seconde partie ...

Methods for isoelectric particle separation

In order to isoelectrically separate particles with a pH-dependent net charge, the particles are exposed in a guiding liquid to electric field forces. The pH value of the guiding liquid is set in such a way that at least one predetermined type of particle is separated from the remaining particles and migrates to a fixing collecting means under the effect of the electric field forces. The collecting means is for example a porous hollow fiber delimited by electrodes which generate the electric field forces and crossed by the guiding liquid together with the sample to be separated. The particles whose isoelectric point matches the pH value of the guiding liquid run unimpeded through the fibers, whereas the remaining particles are pressed against the inner wall of the fiber and are prevented from being carried away with the liquid flow.

Materials classifier, method of using, and method of making

The present invention relates to a method of classifying charged molecules such as proteins for quantitative analysis. An analyte solution of the molecules is subjected to separational forces may be fluid drag and electrophoretic force in opposition. The analyte solution may be subjected to a two-phase process. The two-phase process may add both electrophoretic force based upon molecule charge, and differential mobility resistance based upon molecule mass and/or size.

METHODS AND APPARATUS FOR NONLINEAR MOBILITY ELECTROPHORESIS SEPARATION

L'invention se rapporte à un procédé permettant de déplacer, d'isoler et/ou d'identifier des particules dans un échantillon et consistant à placer ledit échantillon dans un champ électrique à variation spatiale qui agit indépendamment et sélectivement sur les moments d'une particule chargée, dipolaire et d'ordre supérieur dans un milieu. Cette invention concerne plusieurs procédés nouveaux d'utilisation de l'effet de mobilité non linéaire et de celui d'un champ électrique non uniforme pour la séparation préparatoire et l'analyse approfondie de macromolécules présentant une grande variété de dimensions et particulièrement pour la séparation d'ADN et de ses fragments et d'autres molécules biologiques. L'invention se rapporte plus particulièrement à l'utilisation de corrections non linéaires de la mobilité moléculaire dans un champ électrique au sein de milieux, qui sont considérées comme des paramètres importants d'une séparation améliorée et d'un resserrement des pics. L'invention concerne ...

PROCESS FOR THE CONTINUOUS SEPARATION OF MIXTURES OF MICROSCOPICALLY SMALL DIELECTRIC PARTICLES AND DEVICE FOR IMPLEMENTING THE PROCESS

The description relates to a process for separating mixtures of microscopically small dielectric particles in suspension and a device for implementing the process. Prior art separation processes separate particle mixtures according to the differing mobilities of their components. This requires extensive measures to suppress convection in the suspension. In the process of the invention, the particle mixture is forced onto tracks by dielectrophoretic forces or by the flow of the suspension medium. An additional force which, for specific particle types, offsets the force driving the particles onto tracks, separates particles of these types out of the mixture. The device for implementing the process is suitable for system integration. It can be integrated on surfaces of silicon wafers economically and in large numbers. The device is suitable for isolating small particles like biological cells, cell organella, bio-molecules and organic dielectric particles.

Cross-flow split-thin-flow cell

A split thin-flow separations device can include a fluid channel having an inlet zone, an outlet zone, and a transport region between the inlet zone and outlet zone. The inlet zone includes a sample inlet and a carrier fluid inlet which are fluidly separated by an inlet splitter to minimize mixing of fluids from respective inlets in the inlet zone. The transport region can be a substantially open channel. Similar to the inlet zone, the outlet zone can include a sample outlet and a carrier outlet which are fluidly separated by an outlet splitter to segregate portions of a fluid into each of the two outlets as the fluid enters the outlet zone. A plurality of cross-flow inducers can also be oriented along a wall of the fluid channel in the transport region. The cross-flow inducers are oriented to form a cross-flow field across the transport region.

PROCESS AND DEVICE FOR ISOELECTRIC PARTICLE SEPARATION

Electrocapturing flow cell

A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.

PROCESS AND DEVICE FOR ISOELECTRIC PARTICLE SEPARATION

In order to isoelectrically separate particles with a pH-dependent net charge, the particles are exposed in a guiding liquid to electric field forces. The pH value of the guiding liquid is set in such a way that at least one predetermined type of particle is separated from the remaining particles and migrates to a fixing collecting means under the effect of the electric field forces. The collecting means is for example a porous hollow fibre delimited by electrodes which generate the electric field forces and crossed by the guiding liquid together with the sample to be separated. The particles whose isoelectric point matches the pH value of the guiding liquid run unimpeded through the fibres, whereas the remaining particles are pressed against the inner wall of the fibre and are prevented from being carried away with the liquid flow.

APPARATUSES AND METHODS FOR FIELD FLOW FRACTIONATION OF PARTICLES USING ACOUSTIC AND OTHER FORCES

This invention relates generally to the field of field-flow-fractionation. In particular, the invention provides apparatuses and methods for the discrimination of mattes utilizing acoustic force, or utilizing acoustic force with electrophoretic or dielectrophoretic force, in field flow fractionation.

Micromachined electrical field-flow fractionation system

A micromachined system for electrical field-flow fractionation of small test fluid samples is provided. The system includes a microchannel device comprising a first substrate having a planar inner surface with an electrode formed thereon. A second substrate having a planar inner surface with an electrode formed thereon is positioned over the first substrate so that the respective electrodes face each other. An insulating intermediate layer is interposed between the first and second substrates. The intermediate layer is patterned to form opposing sidewalls of at least one microchannel, with the electrodes on the substrates defining opposing continuous boundaries along the length of the microchannel. Inlet and outlet ports are formed in one or both substrates for allowing fluid flow into and out of the microchannel. The microchannel device can be fabricated with single or multiple microchannels therein for processing single or multiple test fluids. During operation, a voltage differential ...

Materials classifier, method of using, and method of making

The present invention relates to a method of classifying charged molecules such as proteins for quantitative analysis. An analyte solution of the molecules is subjected to separational forces may be fluid drag and electrophoretic force in opposition. The analyte solution may be subjected to a two-phase process. The two-phase process may add both electrophoretic force based upon molecule charge, and differential mobility resistance based upon molecule mass and/or size.

Making a matrix of random encoded arrays

Disclosed is an apparatus and method of maintaining chips or arrays of particles, or arrays of particles deposited on chip surfaces, in a designated area on a substrate surface. This is accomplished by having hydrophilic chips or arrays of particles, and a substrate which has a series of hydrophilic regions designed to accommodate the chips/arrays of particles, surrounded by hydrophobic regions. In the presence of aqueous solution, the chips or arrays of particles are held in the designated regions.

AUSTAUSCHBARE KASSETTE ZUR REINIGUNG VON MAKROMOLEKÜLEN

Chips in fluid confinement regions

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (“gray scales”) of ...

Electrocapturing flow cell

A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.

Electrophoretic separation of compounds

A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into. the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

A new electrode design for electrical field flow fractionation

An improved channel for separation of molecular and particulate samples by means of electrical field flow fractionation (FFF) is disclosed. The electrodes therein are made of metal oxide coated flat glass plates and permit the achievement, thereby, all of the seven most desirable features for such an electrical FFF channel, viz. 1) hardness, 2) flatness, 3) smoothness, 4) chemical inertness, 5) durability (does not generate particles), 6) high conductivity, and 7) transparency. In addition, the design of the clamping plates permits high precision in aligning the electrodes for parallelism.

Method and apparatus for continuous annular electrochromatography

Separation of complex mixtures and solutions can be carried out using a method and apparatus for continuous annular electrochromatography. Solutes are diverted radially by an imposed electrical field as they move downward in a rotating chromatographic column.

Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separtion, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods ...

Materials classifier, method of using, and method of making

The present invention relates to a method of classifying charged molecules such as proteins for quantitative analysis. An analyte solution of the molecules is subjected to separational forces may be fluid drag and electrophoretic force in opposition. The analyte solution may be subjected to a two-phase process. The two-phase process may add both electrophoretic force based upon molecule charge, and differential mobility resistance based upon molecule mass and/or size.

Microfluid molecular-flow fractionator and bioreactor with integrated active/passive diffusion barrier

A microfluidic device and method is disclosed for fractionating and/or trapping selected molecules with a diffusion barrier or porous membrane. The device includes a source fluid flow channel and a target fluid flow channel. The target fluid flow channel and the source fluid flow channel meet at cross-channel area and are in fluid communication with each other. A porous membrane separates the source fluid flow channel from the target fluid flow channel in the cross-channel area. A field-force/gradient mechanism may be positioned proximate the porous membrane with or without detection/state monitoring devices.

Mass spectrometry system and method employing measurement/survey scan strategy

A gas chromatography plus mass spectrometry system implements a scan strategy in which each full range scan alternates between a normal measurement mode and a survey mode based on a block/gap map made during the previous scan. Survey mode is used within regions that were determined in the previous scan to lack signal above a predetermined threshold. Spectral data is generated during measurement mode operation. Each scan serves both measurement and mapping functions in a way that avoids mass filter jumps, since each scan is monotonic over the entire scanning range.

Method and apparatus for continuous annular electrochromatography

Separation of complex mixtures and solutions can be carried out using a method and apparatus for continuous annular electrochromatography. Solutes are diverted radially by an imposed electrical field as they move downward in a rotating chromatographic column.

Continuous flow particle concentrator

An apparatus for extracting and concentrating bioagents within a continuously flowing fluid medium includes a flow channel fluid inlet, in which bioagents are concentrated from three dimensions to a two-dimensional transport layer in a preconcentration area. Traveling wave grids cause the preconcentrated bioagents to migrate to one side of the flow channel and then to an extraction port. Each of the traveling wave grids includes a substrate, a collection of closely spaced and parallel electrically conductive electrodes extending across said substrate, and a collection of buses providing electrical communication with the collection of conductive electrodes. A voltage controller provides a multiphase electrical signal to the collection of buses and electrodes of the traveling wave grids. Fluid exits through an outlet port.

Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods ...

FRACTIONATION USING DIELECTROPHORESIS AND FIELD FLOW FRACTIONATION

Apparatus for macromolecule purification

An electrophoresis apparatus, comprises a cartridge configured to be removably mounted in the apparatus, and a separation membrane positioned in the cartridge. The separation membrane has a first side along which a first flow path defined in a first grid element and a second side along which a second flow path defined in a second grid element is provided. Restriction membranes separate buffer flow from the first flow path and the second flow path. The apparatus includes connection blocks which house electrodes and inlets and outlets for buffer flow and sample flows. The cartridge is removable from the connection blocks for replacement with another cartridge.

Electrophoretic separation of compounds

A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.

Electrochromatographic device for use in enantioselective separation, and enantioselective separation medium for use therein

An electrochromatographic device is provided for conducting enantioselective separation of enantiomers. The device is comprised of a conduit containing a monolithic enantioselective separation medium, and may be, for example, a capillary tube or a microchannel in a substrate. The enantioselective separation medium is prepared by copolymerization of (a1) an ionizable chiral monomer or (a2) a chiral monomer and an ionizable comonomer, along with (b) a crosslinking comonomer and (c) a polymerization initiator, in (d) a porogenic solvent. Following ionization, the enantioselective separation medium serves as a charge carrier as well as a chiral separation medium, and further acts as an electroosmotic pump to facilitate the flow of a fluid. The invention also provides methods for preparing the enantioselective separation medium and electrochromatographic devices fabricated therewith.

Continuous flow particle concentrator

An apparatus for extracting and concentrating bioagents within a continuously flowing fluid medium includes a flow channel fluid inlet, in which bioagents are concentrated from three dimensions to a two-dimensional transport layer in a preconcentration area. Traveling wave grids cause the preconcentrated bioagents to migrate to one side of the flow channel and then to an extraction port. Each of the traveling wave grids includes a substrate, a collection of closely spaced and parallel electrically conductive electrodes extending across said substrate, and a collection of buses providing electrical communication with the collection of conductive electrodes. A voltage controller provides a multiphase electrical signal to the collection of buses and electrodes of the traveling wave grids. Fluid exits through an outlet port.

Microfluid molecular-flow fractionator and bioreactor with integrated active/passive diffusion barrier

A microfluidic device and method is disclosed for fractionating and/or trapping selected molecules with a diffusion barrier or porous membrane. The device includes a source fluid flow channel and a target fluid flow channel. The target fluid flow channel and the source fluid flow channel meet at cross-channel area and are in fluid communication with each other. A porous membrane separates the source fluid flow channel from the target fluid flow channel in the cross-channel area. A field-force/gradient mechanism may be positioned proximate the porous membrane with or without detection/state monitoring devices.

Method of continuously separating mixtures of microscopic dielectric particles and apparatus for carrying through this method

A method of separating mixtures of microscopic dielectric particles in sunsions in an apparatus for carrying the method. A mixture of particles is forced onto guide paths by dielectrophoretic forces or by a flow of the suspension medium with an additional force, which is provided to compensate the force causing the particles to move along the guide paths for specific particle species causing the specific particle species to be fed out from the mixture of particles. The apparatus may be integrated on surfaces of silicon wafers at low cost and in mass-production numbers, and is suitable for isolating minute particles such as biological cells, cell organelles, bio molecules as well as organic dielectric particles.

Separation method and apparatus using dielectrophoresis and field flow fractionation

(57)【要約】 本願発明は、物質の分離、特徴付け、操作のための新規装置と新規方法とに関するものである。特に、本発明は、そのような物質を識別及び分離するために、粒状物質および溶解物質の周波数依存性の誘電および導電特性と、懸濁輸送培地の特性の使用を組み合わせている。本装置は、細胞を導入し、取り出すための、少なくとも一の電極素子と少なくとも一の入口ポートと出口ポートとを備えた室を含む。流体の流れにより室を通って運ばれた物質は、その後、エネルギーを与えられた電極により引き起こされる誘電電気泳動（DEP）力によって流体内で分離される。流体の中での分離に続いて、物質は、速度プロフィールに応じた速度で室を通って移動する。物質が室を通って通過した後、物質は特徴的な位置で室の反対側の端から出ていく。本発明の方法は、調査、状態の診断、治療目的で物質を識別及び分離するための装置を使用することを含む。そのような方法の例は、癌細胞の正常細胞からの分離、正常赤血球からの寄生された赤血球の分離、核酸の分離などといった、細胞混合物の分離を含む。

Liquid flowing type carrier electrophoresis apparatus

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Method and apparatus for fractionation using generalized dielectrophoresis and field flow fractionation

The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transmitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to the invention involve using the apparatus for discriminating and separating matter for research, diagnosis of a condition, and therapeutic purposes. Examples of such methods may include separation of mixtures of cells, such as cancer cells from normal cells, separation of parasitized erythrocytes from normal erythrocytes, separation of nucleic acids, and others.

Fraktionierung mittels dielektrophoresis und fraktionierung unter anwendung eines flussfeldes

Verfahren und vorrichtung zur isoelektrischen teilchentrennung

Application of static light to a fluid flow of CNTs for purposes of sorting the CNTs

A method is described that comprises sorting carbon nanotubes (CNTs) within a fluidic flow for a targeted subset of the CNTs. The sorting comprises attracting at least a portion of the CNTs within the fluidic flow in a direction of increasing intensity of an electric field component of a substantially stationary beam of light. The electric field component has a frequency that is less than one or more resonant frequencies of the CNTs within the portion.

APPLICATION OF STATIC LIGHT TO A FLUID FLOW OF CNTs FOR PURPOSES OF SORTING THE CNTs

A method is described that comprises sorting carbon nanotubes (CNTs) within a fluidic flow for a targeted subset of CNTs. The sorting comprises attracting at least a portion of the CNTs within the fluidic flow in a direction of increasing intensity of an electric field component of a substantially stationary beam of light. The electric field component has a frequency that is less than one or more resonant frequencies of the CNTs within the portion.

Ｃｎｔを選別するためにｃｎｔの流体流に静的な光を当てる方法

流体流内のカーボンナノチューブ（ＣＮＴ）をＣＮＴの標的サブセットを得るために選別することを含む方法が説明される。選別は、流体流内のＣＮＴの少なくとも一部分を、実質的に固定した光のビームの電場成分の強度が増大する方向において吸引することを含む。電場成分は部分内のＣＮＴの１つ又は複数の共鳴振動数を下回る振動数を有する。 【選択図】なし

Electrical field flow fractionator

The present disclosure describes a field flow fractionator including (a) a top plate assembly including a top electrically conductive electrode, (b) an o-ring, (c) an electrically insulating frit, (d) an electrically insulating spacer between a bottom surface of the top electrode and the o-ring and the frit, (e) a membrane between the spacer and the frit, and (f) a bottom plate assembly including a bottom electrically conductive electrode.

Austauschbare kassette zur reinigung von makromolekülen

Miniaturized electrothermal flow induced infusion pump

A micropump that pumps liquid using electrothermally-induced flow is described, along with a corresponding self-regulating pump and infusion pump. The micropump has applications in microfluidic systems, such as biochips. The self-regulating infusion pump is useful for administration of large and small volumes of liquids such as drugs to patients and can be designed for a wide range of flow rates by combining multiple micropumps in one infusion pump system. The micropump uses electrode sequences on opposing surfaces of a flow chamber that are staggered with respect to each other. The opposing surfaces include staggered electrodes that have the same phase and same electrode sequence. As such electrodes with the same phase are staggered and not eclipsed.