Hydrophilic activated sorbent extraction disk

A one-piece solid phase extraction article to be used in solid phase extraction analysis, a filtration funnel comprising the one piece solid phase extraction article, and a method of use of the article. The article, device and method provide for improved analysis of hexane extractable material by eliminating the standard use of polar solvents to pre-condition the sorbent materials. The one-piece solid phase extraction article comprises water wettable monomeric phase type sorptive material embedded within a porous matrix. The water wettable monolithic polymeric sorptive material may be a hydrophilic polymeric gel, such as functionalized C-18 sorbent material, and the porous matrix may be fibrous matric, such as a glass fiber. The one-piece solid phase extraction article may further comprise one or more pre-filtration layers which do not contain the sorptive particles.

Method of making ionic liquid mediated sol-gel sorbents

Ionic liquid (IL)-mediated sol-gel hybrid organic-inorganic materials present enormous potential for effective use in analytical microextraction. One obstacle to materializing this prospect arises from high viscosity of ILs significantly slowing down sol-gel reactions. A method was developed which provides phosphonium-based, pyridinium-based, and imidazolium-based IL-mediated advanced sol-gel organic-inorganic hybrid materials for capillary microextraction. Scanning electron microscopy results demonstrate that ILs can serve as porogenic agents in sol-gel reactions. IL-mediated sol-gel coatings prepared with silanol-terminated polymers provided up to 28 times higher extractions compared to analogous sol-gel coatings prepared without any IL in the sol solution. This study shows that IL-generated porous morphology alone is not enough to provide effective extraction media: careful choice of the organic polymer and the precursor with close sol-gel reactivity must be made to ensure effective chemical bonding of the organic polymer to the created sol-gel material to be able to provide the desired sorbent characteristics.

Method and System for Transferring Separation Resin

A method and a system and a container system for transferring separation resin from at least one first container (′) to a second container (′), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of:—preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (′) with a predefined volume of separation resin in a storage solution;—fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;—fluidically connecting (S5) the outlet port (′) of the at least one first container to an inlet port () of the second container;—transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container. 1. A method for transferring separation resin from at least one first container to a second container , wherein said first container is a deformable , single-use separation resin storage container , said method comprising the steps of:preparing the at least one first container by providing a deformable, single-use container comprising an outlet port with a predefined volume of separation resin in a storage solution;fluidizing the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;fluidically connecting the outlet port of the at least one first container to an inlet port of the second container;transferring separation resin from the at least one first ...

GUARD COLUMN AND METHOD FOR PRODUCING GUARD COLUMN

Provided is a guard column including a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, in which the filler is made of porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, and a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more. 1. A guard column , comprisinga filling part having a length of 2.0 cm to 3.5 cm formed of a filler, whereinthe filler includes porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, anda pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.2. The guard column according to claim 1 , wherein the porous silica gel has an average pore size of 25 nm to 35 nm and a specific surface area of 100 m/g to 300 m/g.3. The guard column according to claim 1 , the column is used for size-exclusion chromatography in which a column using the filler containing porous silica gel and a light scattering detector are used.4. A liquid chromatograph comprising the guard column according to claim 1 , a size-exclusion chromatography column filled with a filler containing porous silica gel claim 1 , and a light scattering detector in this order from an upstream side.5. A method of analyzing a polymer compound claim 1 , which comprises:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'analyzing a polymer compound with size-exclusion chromatography using the liquid chromatograph according to .'}6. A method for producing a guard column according to claim 1 , comprising:a step of filling a filler including porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm at a filling pressure of 12 MPa to 40 MPa per 1 cm in length to form a filling part having a length of 2.0 cm to 3.5 cm.7. The guard column according to claim 1 , wherein a diameter of the filling part is 0.4 cm to 0.9 cm.8. The guard column according to ...

Parallel Separation and Washing in Size Exclusion Chromatography Separation or in Desalting of a Target from a Sample

Systems and methods are provided for performing washing of columns in a simulated moving bed chromatography system while separation is being performed in the system. 1. A method for performing size exclusion chromatography separation (SEC) or desalting of a target from a sample in a system comprising:i) providing a system with at least three columns at least two of which are connectable in series at the same time;ii) connecting in series a column containing the target with another of the columns; andiii) washing at least one further column with a washing fluid at the same time as the target is being transported by a flow of buffer from said column containing the target to said another of the columns.2. The method according to claim 1 , further comprising the subsequent step of connecting the outlet of said another column to the inlet of the washed further column and subsequently collecting the target from the outlet of the washed column.3. The method according to claim 1 , further comprising the steps of transporting the target in series from the outlet of said another column in any order through said washed further column and one or more additional columns claim 1 , and finally collecting the target from the last additional column or the washed further column.4. The method in accordance with for a size exclusion chromatography (SEC) or desalting system wherein said at least three columns comprise four columns for separating a target from a sample claim 1 , wherein the target passes through three or four columns before being collected claim 1 , wherein each column has an inlet and an outlet fluidly connectable to a valve arrangement for selectively simultaneously connecting said at least two chromatography columns in series and one of the other chromatography columns to flow of washing fluid claim 1 , wherein said valve arrangement comprises a first inlet for inputting a buffer solution claim 1 , a second inlet for inputting a washing fluid claim 1 , an inlet for ...

Metal components with inert vapor phase coating on internal surfaces

The invention provides metal liquid chromatography components with uniformly coated internal surfaces and methods for achieving the same. The invention addresses the problem of corrosion or interference of metal components in the flow path for LC analyses in which the sample interacts with metal ions or surfaces. The invention also alleviates the difficulties in coating very long metal tubes and very small metal channels with an inert, continuous coating that adheres well to metal surfaces. The metal flow path is rendered inert by the coating, and thus compatible with bioanalytical separations, for example, by using a vapor phase deposition process to coat the inner surfaces with a coating that continuously covers all metal surfaces in the flow path.

SINGLE USE SLURRYING AND CHROMATOGRAPHY SYSTEMS

Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration. 1. A disposable container for chromatography comprising:a flexible body configured to receive a chromatography resin, the flexible body comprising:a separation barrier that separates the flexible body into a filtration portion and a drainage portion;wherein the separation barrier retains the chromatography resin separately from the drainage portion during use while allowing fluids to pass therethrough.2. The disposable container of further comprising:at least one agitator disposed within the flexible body and configured to be raised and lowered therein.3. The disposable container of claim 2 , wherein the agitator in a raised position permits the chromatography resin to operate in a packed-bed configuration.4. The disposable container of claim 2 , wherein the agitator in a lowered position permits the chromatography resin to operate in a slurry configuration.5. The disposable container of further comprising:at least one probe coupled to the flexible body and operative to ...

Temperature-responsive monolithic porous body, method for producing same, and temperature-responsive chromatography method using same

A temperature responsive monolithic porous material is obtained that comprises a polymer having a hydration ability that changes in a temperature range of 0 to 80° C. and being immobilized to a surface of the porous material at a high density by binding an atom transfer radical polymerization initiator to a surface of the porous material, and inducing a growth reaction of a polymer, having a hydration ability that changes in a temperature range of 0 to 80° C., from the initiator using an atom transfer radical process under a presence of a catalyst.

TEMPERATURE-RESPONSIVE MONOLITHIC POROUS BODY, METHOD FOR PRODUCING SAME, AND TEMPERATURE-RESPONSIVE CHROMATOGRAPHY METHOD USING SAME

A temperature responsive monolithic porous material is obtained that comprises a polymer having a hydration ability that changes in a temperature range of 0 to 80° C. and being immobilized to a surface of the porous material at a high density by binding an atom transfer radical polymerization initiator to a surface of the porous material, and inducing a growth reaction of a polymer, having a hydration ability that changes in a temperature range of 0 to 80° C., from the initiator using an atom transfer radical process under a presence of a catalyst. 1. A temperature responsive monolithic porous material comprising a polymer having a hydration ability that changes in a temperature range of 0 to 80° C. and being bound to a surface of the porous material at a density of 0.01 molecular chain/nmor higher.2. The temperature responsive monolithic porous material of claim 1 , wherein the porous material consists of silica.3. The temperature responsive monolithic porous material according to claim 1 , wherein an amount of bound-polymer on the surface of the porous material is 0.2 to 10.0 mg/m.4. The temperature responsive monolithic porous material according to claim 1 , wherein a polymer molecular chain is non-crosslinked.5. The temperature responsive monolithic porous material according to claim 1 , wherein the polymer is one or a plurality of poly-N-substituted acrylamide derivative claim 1 , poly-N-substituted methacrylamide derivative claim 1 , their copolymer claim 1 , polyvinylmethyl ether claim 1 , a partially acetylated polyvinyl alcohol.6. The temperature responsive monolithic porous material according to claim 1 , wherein the polymer is poly-N-isopropylacrylamide.7. The temperature responsive monolithic porous material according to claim 5 , wherein the polymer is a copolymer containing a hydrophilic molecule claim 5 , a hydrophobic molecule and an ionic molecule in a polymer molecular chain claim 5 , at a range that retains a feature of the hydration ability to ...

TRANSITION ANALYSIS METHOD FOR CHROMATOGRAPHY COLUMN QUALIFICATION

The present disclosure is directed to a method of operating a chromatography column. This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is determined based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. The height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value. If during routine column monitoring, an adverse trend in HETP is observed or the control limits are exceeded, the eluate product quality, column process performance, and/or impurity removal data should be evaluated to ensure product quality for the identified batch. Should any of the product quality or column performance fail the criteria set, appropriate corrective action, such as conditioning, repacking or replacing the column, and qualification should be performed prior to release for further use. 117-. (canceled)19. The system of claim 18 , wherein the chromatography column is conditioned claim 18 , replaced claim 18 , or repacked based on said assessing.20. The system of claim 18 ,wherein the detector is configured to collect column outlet signals and accumulated flow parameters at two or more intervals of a corresponding mobile phase transition front during one or more subsequent uses of the chromatography column packing;wherein the computing device comprises processor and a non-transitory computer-readable medium with instructions stored thereon, which, when executed by the processor, perform steps comprising:a) said determining and said calculating using the column outlet signal and ...

HPLC COLUMN

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use. 137.-. (canceled)38. A chromatography column having a tubular body with an inner diameter , the column having opposing inlet and outlet ends , the column comprising:a bed of chromatographic media that has just been compressed to its maximum axial compression packing pressure during formation of the chromatographic bed;an upstream and downstream frit inside the tubular body and located on respective upstream and downstream ends of the bed of chromatographic media;a piston having opposing, upstream and downstream ends, the downstream end of the piston urging the upstream frit against the bed of chromatographic media and maintaining the axial compression packing pressure on the bed of chromatographic media, with no spring interposed between the first frit and the piston, the upstream end of the piston having an annular surface encircling a longitudinal axis of the column and facing away from the upstream frit;a fluid seal interposed between the piston and the inner diameter of the tubular body;a locking sleeve with multiple-parts that are connected together to encircle the inlet end of the column, each part of the locking sleeve having sleeve threads located thereon to form a threaded surface encircling the longitudinal axis;an annular locking collet having collet threads located to threadingly engage the ...

Materials Transport Device for Diagnostic and Tissue Engineering Applications

Devices that can transport biological materials are described. The devices incorporate capillary channeled fibers that can effectively transport living cells as well as other biological materials such as nutrients, growth factors, waste materials, etc. The devices can include a sorptive material at one end of the fibers that can improve transport of materials through the devices. The devices can differentially transport different cell types, particularly when the fibers are held in a vertical orientation. Diagnostic devices that incorporate the capillary channeled fibers are described that can be utilized to separate cell types from one another. Tissue engineering scaffolds that incorporate the capillary channeled fibers are described that can more efficiently transport materials into and out of the scaffolds. 1. A device for transporting biological materials comprising:a plurality of capillary channeled fibers, each capillary channeled fiber having a longitudinal axis along a length of the fiber, an outer surface, and a non-circular cross section that is perpendicular to the longitudinal axis of the fiber, each capillary channeled fiber including a plurality of co-linear open-topped channels extending along the entire length of the fiber, each open-topped channel being open at the outer surface of the fiber, each open-topped channel being defined by two opposed walls, each wall extending in a first direction that is along the length of and parallel to the longitudinal axis of the fiber and each wall also extending in a second direction that is perpendicular to the longitudinal axis of the fiber, with each wall forming a side wall of one channel and each wall forming a part of the exterior surface of an open-topped fiber, the plurality of capillary channeled fibers being held adjacent to one another and generally aligned with one another in a bundle such that at least one closed channel is formed between a first open-topped channel of a first fiber and a second open ...

SIMPLE SINGLE-STEP POROUS POLYMER MONOLITH FOR DNA EXTRACTION

A method and microfluidic device with a porous polymer monolith in a channel of the device with capture affinity element (such as an oligonucleotide complementary to a DNA target from the KPC antibiotic resistance gene) on the monolith surface. 1. A method for creating a monolith in a void space of a microfluidic device , comprising:filling the internal void spaces of the monolith with a polymerization mixture comprising one or more photopolymerizable monomers, a photo initiator, and a capture affinity element;masking microfluidic device to prevent light from reaching the internal void spaces with the exception of a gap to allow light to reach a preselected portion of the void spaces;exposing the microfluidic device to light to polymerize polymerization solution in the preselected portion to form in the preselected portion a porous polymer monolith with capture affinity element on a polymer monolith surface, and with the polymer monolith anchored on a device surface within the preselected portion;rinsing unpolymerized polymerization solution from the internal void spaces.2. The method of wherein the capture affinity element is a capture oligonucleotide.3. The method of wherein the polymerizable mixture comprises one or more photopolymerizable acrylate monomers.4. The method of wherein the microfluidic device is exposed to ultra-violet light.5. The method of wherein the filling claim 1 , masking claim 1 , exposing claim 1 , and rinsing steps are conducted once in a single step.6. The method of wherein the acrylate monomers are one or more from the group of PEGDA and EDMA.7. The method of wherein the capture oligonucleotide is complementary to a target DNA sequence.8. The method of wherein the capture oligonucleotide is complementary to a target from a gene from a microorganism.9. The method of wherein the capture oligonucleotide is complementary to a target from a gene from DNA of a bacterium.10. The method of wherein the capture oligonucleotide is complementary to a ...

Chromatography Media And Method

Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes

R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

Modular-type analysis system

In the non-volatile storage unit ( 341 ) of the local control unit ( 34 ) of each unit ( 3 to 6 ) configuring an LC system, periodic inspection information, such as the installation date and the next inspection scheduled date, is stored at an appropriate time. When an analysis is carried out, in the system control unit ( 2 ) which controls each unit, a periodic inspection notification processing unit ( 211 ) collects periodic inspection information from each unit ( 3 to 7 ) upon power on or start up, compares the next inspection scheduled date with the current time by the real-time clock ( 212 ), and determines a unit for which a periodic inspection notification is required. If there is a unit that requires a periodic inspection notification, a notification request is sent to the unit, and the local control unit ( 34 ) of the unit outputs an indication prompting periodic inspection to the display unit ( 35 ). As a result, even if the installation times are different for each unit and even if each unit does not have a real-time clock, it is possible to notify a user when a predetermined age of service has passed from the installation time of a given unit.

Liquid chromatography technique

LC techniques are disclosed. The LC technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150 degree Celsius, pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone.

Column packing material for supercritical fluid chromatography, column for supercritical fluid chromatography and preparation method therefor

A column packing material for supercritical fluid chromatography which allows good peak shape to be obtained in the analysis of free fatty acids, etc. and in the analysis of agricultural chemicals, etc., and has excellent durability for repeated analysis. The column packing material for supercritical fluid chromatography includes polymer particles containing cross-linked polymer, the degree of swelling of which after absorbing tetrahydrofuran and the degree of swelling of which after absorbing methanol are both 1.4 or less.

Vacuum liquid extraction and purification systems and methods

The invention features compact systems and methods for vacuum liquid purification and extraction of a liquid sample. 1. A vacuum system for purification of and extraction of at least one substance from a liquid sample comprising:a rotatable base for rotation between at least a first position and a second position;at least one assay column assembly having at least a first assay column and at least one second assay column;at least one Stage 1 solvent reservoir and having at least one easy connect fitting adapted for fluidic and reversible connection to the first assay column;at least one Stage 1 manifold mounted on a Stage 1 portion of the rotatable base and having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column;at least one Stage 2 reservoir and having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column;at least one Stage 2 manifold mounted on a Stage 2 portion of the rotatable base and having at least one easy connect fitting adapted for fluidic and reversible connection with the second assay column;wherein the first assay column has an easy connect fitting disposed at a first entry which is adapted for fluidic and reversible connection to the easy connect fitting of the Stage 1 solvent reservoir;wherein the first assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which is adapted for fluidic and reversible connection to the second assay column;wherein the second assay column has an easy connect fitting disposed at a first entry of the second assay column which is adapted for fluidic and reversible selective alternate connection with the first assay column and the Stage 2 manifold;wherein the second assay column has an easy connect fitting disposed at a first exit opposing the first entry of the first assay column which adapted for fluidic and reversible selective alternate connection ...

COMPOSITIONS, METHODS AND MICROFLUIDICS DEVICE FOR TELOMERASE BASED IN VITRO DIAGNOSTIC ASSAYS FOR DETECTING CIRCULATING TUMOR CELLS (CTC)

A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5′ and 3′ ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3′ ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample. Because the system is non-lytic, the testing can be repeated on the cells which remain viable in culture. Also provided is a method for enriching test samples having circulating tumor cells and a microfluidics device suitable for CTC-specification identification and enumeration. 1. A method for detecting circulating tumor cells in vitro , said method comprising (i) a first replication-defective adenoviral particle having an adenoviral capsid in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5′ ITR, a tumor-specific promoter which is specifically activated in the presence of circulating tumor cells, and an adenoviral 3′ ITR, wherein said adenoviral particle is rendered replication defective and non-lytic by a deletion in one or more adenoviral early genes; and', '(ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3′ ITR;, '(a) combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and ...

Methods for Chromatography Resin Slurry Determination

The present invention relates to methods for determining a slurry concentration for a slurry of a chromatography resin during a column packing. The present invention provides a more accurate and consistent method for determining a slurry concentration for packing a chromatography column. The method of the present invention utilizes an automated pump to provide a controlled flow rate to consolidate a resin sample. Based on the consolidated resin, a slurry concentration can be determined. The determined slurry concentration can be utilized to pack the resin in a chromatography column with highly accurate bed heights. 1. A method of determining a slurry concentration , comprising:adding a slurry comprising a resin to a first chromatography column;pumping a volume of liquid through the chromatography column at a constant flow rate to form a consolidated resin, wherein the volume of liquid is pumped through the chromatography column by an automated pump system;upon completion of the pumping step, measuring a bed height of the consolidated resin; anddetermining a slurry concentration from the measured bed height.2. The method of claim 1 , wherein the liquid is deionized water claim 1 , distilled water claim 1 , purified water claim 1 , a saline solution claim 1 , or an organic solvent.3. The method of claim 1 , wherein the step of adding the slurry further comprises adding water to the chromatography column and allowing the slurry to settle for about five minutes to about one hour prior to pumping.4. The method of claim 1 , further comprising utilizing the determined slurry concentration to pack a second chromatography column.5. The method of claim 4 , wherein the second chromatography column comprises a consolidation rate and wherein the flow rate is equivalent to the consolidation rate.6. The method of claim 1 , wherein the automated pump system comprises a syringe operatively coupled to a pump.7. A method for determining a slurry concentration for a slurry of a ...

CHROMATOGRAPHIC CASSETTE

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute. 1. An adsorptive device comprising:a shell including a chamber;chromatographic media disposed within the chamber;a distribution network fluidly coupled to the chromatographic media; andan inlet port and an outlet port coupled to the distribution network.2. The adsorptive device of claim 1 , wherein the chamber comprises a lattice; andthe chromatographic media comprises adsorptive beads.3. The adsorptive device of claim 1 , wherein the chromatographic media comprises a chromatographic adsorptive web.4. The adsorptive device of claim 1 , wherein the chromatographic media comprises one of:a monolithic block;a polymeric media;a hydrogel media;a compressible adsorptive media; anda semi-compressible adsorptive media.5. The adsorptive device of claim 1 , wherein the shell is manufactured by at least one of:3-D printing;molding; andextrusion.6. The adsorptive device of claim 5 , wherein the 3-D printing comprises FDM printing.7. The adsorptive device of claim 1 , wherein the adsorptive device further comprises:a top planar surface; anda bottom planar surface.8. The adsorptive device of claim 7 , wherein the distribution network comprises a planar distributor and provides a direction of fluid flow through the chromatographic media which is perpendicular to the top and bottom planar surfaces of the adsorptive ...

CHROMATOGRAPHY COLUMN FRAME AND METHOD OF CONDUCTING MAINTENANCE ON AND PACKING OF A CHROMATOGRAPHY COLUMN

The invention relates to a chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder means connected to said support arrangement. Said holder means is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction. The invention also relates to method of conducting maintenance on and packing of a chromatography column. 1. A method of conducting maintenance on and packing of a chromatography column , the method comprising:providing a chromatography column comprising; a tubular housing;a first end unit removably connected to a first end of the tubular housing; a second end unit removably connected to a second end of the tubular an adaptor assembly moveable within said tubular housing; and 'an adaptor rod connected to said adaptor assembly, which adaptor rod is arranged to extend through an opening in the first end unit;', 'housing;'}providing a frame comprising at least two legs; 'disconnecting the first end unit or the second end unit from the tubular housing;', 'a support arrangement connected to said at least two legs; and a holder connected to said support arrangement;'} arranging a driver on the adaptor rod; and', 'activating the driver, so that the adaptor rod together with the first end unit and the adaptor assembly move vertically upwards separating the first end unit and the adaptor assembly from the tubular housing or also separating the tubular housing from the second end unit., 'positioning the adaptor rod in said holder, so that the adaptor rod is prevented from moving in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction;'}2. The method according to claim 1 , further comprising:performing maintenance on the chromatography column.3. The method according to claim 1 , further comprising:supplying a packing ...

ZWITTERIONIC COMPOUNDS AS GAS CHROMATOGRAPHIC COLUMN STATIONARY PHASES

A gas chromatographic (GC) column using a zwitterionic compound and methods of use thereof are disclosed herein. The volatile free acids were observed to strongly retain on these zwitterionic compounds-based columns with excellent peak symmetry. By carefully tuning the structures of these zwitterionic compounds, different selectivity toward volatile free acids was demonstrated. These stationary phases possess a wide working range with thermal stabilities at higher temperatures. 1. A method of chromatographic analysis or separation , the method comprises:separating an analyte by a column; wherein the column comprises a zwitterionic compound as its stationary phase.2. The method of claim 1 , wherein the column is a gas chromatographic (GC) column comprising a zwitterionic compound as its stationary phase claim 1 ,wherein the zwitterionic compound comprises one or more cationic systems and one or more anionic groups;{'sub': 3', '2, 'sup': −', '6', '−', '6, 'wherein the one or more anionic groups comprises at least a sulfonate, phosphonate, or a group containing a —SO or —P(OR)O group; where Ris H or substituted or unsubstituted alkyl group; and'}wherein the cationic system is a quaternary ammonium, phosphonium, sulfonium, guanidinium compound, or a positively charged heterocyclic group derived from a 5 or 6 membered heterocyclic group having at least one nitrogen or sulfur atom.3. The method of claim 1 , wherein the column is at a temperature from about 0° C. to about 120° C.7. The method of claim 6 , wherein the organic molecule is not derivatized before the sample is input into the GC column.8. The method of claim 6 , wherein the organic molecule is a C-C claim 6 , C-C claim 6 , C-C claim 6 , C-C claim 6 , C-Cester claim 6 , alcohol claim 6 , free acid claim 6 , ether claim 6 , aldehyde claim 6 , amine claim 6 , or mixture thereof and is volatile at room temperature or a temperature from about 0° C. to 50° C.9. The method of claim 6 , the method further comprises ...

Method of Separating Ions

The present disclosure relates generally to a method of separating ions according to their ion mobility, comprising (i) accumulating a first population of ions in a first region of an ion mobility separator, (ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator, and (iii) accumulating a second population of ions in said first region of said ion mobility separator whilst said first population of ions are being separated according to their ion mobility in said ion mobility separator. 1. A method of separating ions according to their ion mobility , comprising:(i) accumulating a first population of ions in a first region of an ion mobility separator;(ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator; and(iii) accumulating a second population of ions in said first region of said ion mobility separator whilst said first population of ions are being separated according to their ion mobility in said ion mobility separator,wherein said ion mobility separator comprises a second region downstream of said first region, and step (ii) comprises separating said first population of ions according to their ion mobility in said first and second regions of said ion mobility separator.2. A method as claimed in claim 1 , wherein step (ii) comprises accumulating said second population of ions in an accumulation region upstream of said first region claim 1 , whilst said first population of ions are being separated according to their ion mobility in said first and second regions of said ion mobility separator.3. A method as claimed in claim 2 , wherein step (iii) comprises transferring said second population of ions from said upstream accumulation region and into said first region claim 2 , and continuing to pass ions through said upstream accumulation region and into said first region so as to accumulate said second population of ions ...

LOW POLLUTANT DIALYSIS SOLUTION

The present invention relates to a method for determination of pollutants and leachables in dialysis solutions by stir bar sorptive extraction comprising the steps of 1. Method for determination of pollutants and leachables in dialysis solutions by stir bar sorptive extraction comprising the steps ofa. Conditioning of the stir bar coated with a sorptive materialb. Stirring the dialysis solution with a coated stir barc. Desorption of pollutants and leachables from the coated stir bar.2. Method of further comprising the step ofd. Analysis of the pollutants and leachables by GC-MS.3. Method of claim 1 , wherein the conditioning step a) comprises washing in a mixture comprising methanol and dichloromethane.4. Method of claim 3 , wherein the conditioning step a) additionally comprises ultrasonic treatment.5. Method of claim 1 , wherein the sorptive material claim 1 , of the stir bar is poly-dimethylsiloxane (PDMS).6. A process for validating a batch of a dialysis solution in a polymer container comprising the steps of{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a. determining the total amount of diethyl phthalate or dibutyl phthalate in a sample of the batch after heat sterilization by the method according to , and'}b. validating the batch for distribution only if the sample of the batch is determined in step a) to contain less than 150 ng/L by weight of a leachable or pollutant.7. The process of claim 6 , wherein the batch is validated for distribution only if the sample of the batch is determined in step a) to contain less than 150 ng/L of diethylphthalate or dibutyl phthalate.8. The process of claim 7 , wherein the batch is validated for distribution only if the sample of the batch is determined in step a) to contain less than 100 ng/L of diethylphthalate or dibutylphthalate.9. Process for validating primary packaging material for dialysis solutions comprising the steps of:a. filling a dialysis solution in a primary packaging material,b. heat sterilizing the ...

Device for substance separation

The invention relates to a device for substance separation with monolithic sorbents which can be produced by means of 3-D printing. They comprise pressure- and solvent-stable thermoplastics.

Detection System and Method with Nanostructure Flow Cell

A system and method for determining concentration of a constituent of a sample fluid includes a flow cell with a light source emitting incident light to a proximal end thereof. Media disposed within the flow cell supports nanostructures that are substantially transparent in at least a portion of the incident light spectrum. The nanostructures adsorb or absorb the constituent to attain a concentration that is a multiple of the concentration of the constituent in the sample fluid. A sensor detects transmitted light exiting from the media, and generates outputs corresponding to a spectrum of the transmitted light. A processor captures the sensor outputs and compares the incident light spectrum to the transmitted light spectrum to generate an absorbance spectrum. The absorbance spectrum is used to calculate the concentration in the nanostructures, which is then used with the predetermined multiple to calculate the sample concentration. 1. A system for optically determining concentration of a constituent of a sample fluid in real time , the system comprising:a flow cell configured to convey the sample fluid therethrough from a proximal end to a distal end;a light source disposed in optical communication with the proximal end of the flow cell, the light source configured to emit incident light of an incident light spectrum into the flow cell;media disposed within the flow cell;nanostructures supported by the media, the nanostructures being substantially transparent in at least a portion of the incident light spectrum, the nanostructures configured to adsorb or absorb the constituent to attain a nanostructures concentration that is a predetermined multiple of a sample concentration of the constituent in the sample fluid passing through the flow cell;at least one sensor disposed at the distal end of the flow cell, the sensor configured to detect transmitted light exiting from the media, and to generate one or more outputs corresponding to a transmitted light spectrum of the ...

SORBENT TUBE APPARATUS

A sorbent tube apparatus () for high-pressure fluid sample analysis, the sorbent tube apparatus () comprising a pressurisable housing () having first and second fluid ports () and defining a fluid chamber () therein; and a sorbent tube () mountable within the pressurisable housing (), the sorbent tube () extending from one of the first and second fluid ports () and spaced apart from the other of the first and second fluid ports () to be in fluid communication with the fluid chamber (), thereby enabling in use pressure equalisation between the sorbent tube () and fluid chamber (). A method of analysing high-pressure fluid, an analytic probe apparatus, a further sorbent tube apparatus and method of preventing or limiting damage to a sorbent tube during high-pressure fluid sampling are also provided. 1. A sorbent tube apparatus for high-pressure fluid sample analysis , the sorbent tube apparatus comprising:a pressurisable housing havingfirst and second fluid ports and defining a fluid chamber therein; anda sorbent tube mountable within the pressurisable housing, the sorbent tube extending from one of the first and second fluid ports and spaced apart from the other of the first and second fluid ports to be in fluid communication with the fluid chamber, thereby enabling in use pressure equalisation between the sorbent tube and fluid chamber.2. The sorbent tube apparatus as claimed in claim 1 , wherein the sorbent tube is mounted so as to be cantilevered to the said one of the first or second fluid ports.3. The sorbent tube apparatus as claimed in claim 2 , wherein the said other of first or second fluid ports is positioned in alignment with a free end of the sorbent tube.4. The sorbent tube apparatus as claimed in claim 2 , further comprising a mounting connector engageable with the sorbent tube and the said one of the first or second fluid ports.5. The sorbent tube apparatus as claimed in claim 4 , wherein the mounting connector is a mounting sleeve.6. The sorbent tube ...

Method for manufacturing monoclonal antibody using yeast, and screening method

Disclosed is a method for manufacturing a monoclonal antibody without using animal individuals. This method includes a step of introducing a DNA fragment comprising a gene that encodes a secretory signal, a gene that encodes a nanobody, and a gene that encodes a peptide barcode, or a vector containing the DNA fragment, into a yeast cell; and a step of collecting a polypeptide comprising the nanobody and the peptide barcode that has been expressed in the cell and secreted to the outside of the cell. According to the method, it is possible to manufacture a monoclonal nanobody more efficiently in a shorter period of time without using animal individuals.

USE OF VAPOR DEPOSITION COATED FLOW PATHS FOR IMPROVED CHROMATOGRAPHY OF METAL INTERACTING ANALYTES

A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit. 1. A chromatographic column , comprising:an uncoated tube comprising stainless steel or titanium or both; anda coated frit comprising a coating, wherein the coated frit is disposed proximate to an inlet of the tube,wherein the uncoated tube does not have the coating, andthe coating reduces binding of one or more sample components to the coated frit relative to binding of the one or more sample components to the uncoated tube.2. The chromatographic column of claim 1 , wherein the coating is an organosilica.3. The chromatographic column of claim 2 , wherein the organosilica comprises an alkylsilyl.5. The chromatographic column of claim 1 , further comprising a second coated frit disposed within the uncoated tube.6. The chromatographic column of claim 1 , wherein the coated frit includes an underlying substrate of stainless steel.7. The chromatographic column of claim 1 , wherein the coated frit includes an underlying substrate of titanium.8. The chromatographic column of claim 1 , wherein the uncoated tube has a length to diameter ratio of between 40 and 4.9. The chromatographic column of claim 1 , wherein the coating has a thickness of at least 100 Å.10. The chromatographic column of claim 4 , wherein a vapor reagent used to form coating is bis( ...

Packing of Chromatography Columns

Disclosed herein is a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column. 1. A method for preparing a connected plurality of packed chromatography columns , comprising the steps of:a) providing dry chromatography medium particles and preparing a plurality of aliquots of said particles, which aliquots differ from each other by less than 5.0 percent by weight;b) providing a plurality of substantially identical chromatography columns and packing one of said aliquots into each chromatography column to obtain a plurality of packed chromatography columns; andc) fluidically connecting said plurality of packed chromatography columns in parallel.2. The method of claim 1 , wherein said aliquots differ from each other by less than 2.0 percent by weight claim 1 , such as by less than 1.0 percent by weight.3. The method of claim 1 , wherein in step a) said aliquots are prepared from a single homogeneous batch of dry chromatography medium particles.4. The method of claim 1 , further comprising claim 1 , after step a) and before step b) claim 1 , a step a′) of separately suspending said aliquots in a packing liquid.5. The method of claim 1 , further comprising claim 1 , after step b) or after step c) claim 1 , a step b′) of supplying a reswelling liquid to said plurality of packed chromatography columns.6. The method of claim 1 , wherein step c) further comprises fluidically connecting said plurality of chromatography columns to a single sample inlet.7. A method for manufacturing a chromatography subsystem comprising a plurality of chromatography columns connected in parallel claim 1 , said method ...

LIQUID CHROMATOGRAPHY TECHNIQUE

Liquid chromatography techniques are disclosed. Specifically, the liquid chromatography technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid-contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150° C., pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, and combinations thereof 1. A liquid chromatography technique , comprising:providing a liquid chromatography system having a coated stainless steel fluid-contacting element; andtransporting a fluid to contact the coated stainless steel fluid-contacting element;wherein the fluid includes adenosine triphosphate;wherein the coated stainless steel fluid-contacting element has a coating, the coating including carbon, silicon, oxygen, and hydrogen.2. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a frit.3. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a fitting.4. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a pump head.5. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a valve.6. The technique of claim 1 , wherein the liquid chromatography system has a stationary phase claim 1 , the stationary phase having particles having a size of less than 6 micrometers.7. The technique of claim 1 , wherein the liquid chromatography system has a stationary phase claim 1 , the stationary phase having particles being hydrophobic.8. The technique of claim 1 , wherein the liquid chromatography system includes a solvent ...

MONOLITHIC COLUMN CHROMATOGRAPHY

Provided herein are methods of liquid column chromatography in which preparative chromatography is performed in-line with analytical chromatography. In particular aspects a monolithic preparative column is used to purify an analyte of interest from a mixture of other substances by applying the mixture to the column, reversing the flow through the column to elute the analyte, which is applied to an analytical column provided in-line with the preparative column. In other aspects, a single monolithic column is used to perform both the preparative chromatography and analytical chromatography steps in succession. In another aspect, a chromatography system is provided to perform preparative and analytical chromatography using a single monolithic column. 1. A chromatographic method for detecting one or more analytes in a body fluid sample containing one or more other substances , said method comprising:a) applying a body fluid sample to a monolithic chromatography column comprising a monolithic sorbent having macropores and mesopores;b) applying a first mobile phase to said monolithic chromatography column after applying said body fluid under conditions such that one or more analytes are retained on said monolithic chromatography column and one or more other substances present in said body fluid sample are removed, wherein said one or more retained analytes have a molecular weight of less than about 5,000 Daltons;c) eluting said one or more retained analytes by applying a second mobile phase to said monolithic chromatography column; andd) detecting said one or more analytes eluted in step c).2. The method of claim 1 , wherein said second mobile phase is applied to said monolithic chromatography column in the opposite direction of the flow of step (b).3. The method of claim 1 , wherein said body fluid sample comprises an isolated body fluid selected from the group consisting of blood claim 1 , plasma claim 1 , serum claim 1 , bile claim 1 , saliva claim 1 , urine claim 1 , ...

LOW PRESSURE ANION EXCHANGE CHROMATOGRAPHY-TURBIDIMETRIC METHOD FOR SIMULTANEOUS ONLINE ANALYSIS OF TRACE SULFIDE AND CHLORIDE IN WATER SAMPLES

The present invention provides a low pressure anion exchange chromatography—turbidimetric method for simultaneous online analysis of trace S and Cl in water samples using an apparatus comprising a low pressure pump, a sample valve, a sample loop, a low pressure anion chromatographic column, a reactor, an optical flow cell, an optical detector, a computer system, a mixer, a sample flow path, a propelling solution flow path, and a color developer solution flow path, the method comprising: (a) mapping a baseline; (b) mapping spectrogram of S and Cl in test samples; (c) mapping standard working curves; and (d) calculating the concentrations of S and Cl in the test samples based on the peak heights of S and Cl in the spectrogram and the regression equations of standard working curves. In this method, a chromatography method is combined with a turbidimetric method for the first time to realize simultaneous online analysis of trace S and Cl in water samples, and the method is endowed with the advantages of fast analysis speed, high analysis efficiency and low analysis costs. 1123456789. A low pressure anion exchange chromatography—turbidimetric method for simultaneous online analysis of trace Sand Cl in water samples using an apparatus comprising a low pressure pump () , a sample valve () , a sample loop () , a low pressure anion chromatographic column () , a reactor () , an optical flow cell () , an optical detector () , a computer system () , a mixer () , a sample flow path , a propelling solution flow path , and a color developer solution flow path , the method comprising:{'sub': 0', '0, 'b': 1', '3', '2', '1', '9', '1', '3', '2', '3', '9', '4', '9', '6', '5', '7', '8, '(a) setting the apparatus in sample injection state, in which a blank sample (S) is driven by the low pressure pump () to enter the sample loop () through the sample flow path and the sample valve (); and then setting the apparatus in analytical state, in which a color developer solution (R) is driven by ...

Periodic Countercurrent Chromatography Separation of Plasmids

A method of continuous separation of a plasmid from a process feed in an apparatus with at least three chromatography columns packed with separation matrix particles, wherein while one chromatography column is loaded with the process feed, another chromatography column is eluted with an eluent to recover the separated plasmid, and yet another chromatography column is eluted with a further eluent to remove contaminants. 1. A method of continuous separation of a plasmid from a process feed comprising separating the plasmid in an apparatus with at least three chromatography columns packed with separation matrix particles , wherein while one chromatography column is loaded with the process feed , another chromatography column is eluted with an eluent to recover the separated plasmid , and yet another chromatography column is eluted with a further eluent to remove contaminants.2. The method of claim 1 , comprising the steps of:a) conveying the process feed comprising the plasmid through a first chromatography column comprising a packed bed of separation matrix particles;b) conveying an eluent through said first chromatography column, recovering the eluent with the plasmid after passage of the first chromatography column; and conveying said process feed through a second chromatography column packed with the same separation matrix as the first chromatography column; andc) conveying a further eluent through said first chromatography column; conveying an eluent through said second chromatography column, recovering the eluent with the plasmid after passage of the second chromatography column; and conveying said process feed through a third chromatography column packed with the same separation matrix as the first and second chromatography columns.3. The method of claim 2 , wherein after step c) claim 2 , steps a)-c) are repeated for the duration of the continuous process.4. The method of claim 3 , wherein in the repetitions:step a) further comprises conveying a further eluent ...

STACKABLE PLANAR ADSORPTIVE DEVICES

Adsorptive bed devices include a scaffold in housing having a stress absorbing rigid structure and open cells filled with adsorptive beads. The scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow and transfers a portion of the induced compressive stress to the housing. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal. The adsorptive media includes multiple layers of planarly cohesive media and when operated in a shallow bed configuration possesses significant tensile strength along its planar dimensions enabling it to support the hydraulic pressures that will be generated by the fluids being processed through the adsorptive devices. 1. An adsorptive bed to receive a liquid flow comprising:a housing having a first surface; a stress absorbing substantially planarly cohesive rigid structure; and', 'a plurality of open cells disposed within the rigid structure;, 'a scaffold disposed within the housing in contact with the first surface comprisinga plurality of adsorptive beads filling the plurality of open cells forming a packed bed of the plurality of adsorptive beads; andwherein the scaffold restricts movement of the plurality of adsorptive beads, absorbs tensile stress induced by a hydraulic pressure within the bed, absorbs compressive stress induced on the plurality of adsorptive beads by a hydraulic pressure gradient along a direction of the liquid flow and transfers a portion of the induced compressive stress to the first surface of the housing.2. The adsorptive bed of claim 1 , wherein the rigid structure comprises a plurality of stacked planarly cohesive separator sheets claim 1 , each of the plurality of stacked planarly ...

SYSTEM AND METHOD OF APPLIED RADIAL TECHNOLOGY CHROMATOGRAPHY

A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250 Å to about 5000 Å, and each bead having an average radius between about 100 μm to about 250 μm. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column. 1. A radial flow chromatography column comprising:a plurality of beads, with each bead comprising one or more pores therein, andinterstitial channels formed between the beads,wherein each pore has a diameter of about 250 Å to about 5000 Å,wherein at least about 80% of the plurality of beads have a diameter of about 200 μm to about 500 μm andwherein the beads have an average radius R of about 100 μm to about 250 μm.2. The radial flow chromatography column of claim 1 , wherein the bead is made of a polymer claim 1 , glass claim 1 , alumina claim 1 , silica claim 1 , controlled pore glass (CPG) claim 1 , cellulose claim 1 , encapsulated iron particles claim 1 , encapsulated CPG claim 1 , or encapsulated silica.3. The radial flow chromatography column of claim 2 , wherein the bead is a polymer bead.4. The radial flow chromatography column of claim 1 , wherein any beads having r<0.414 R have been removed.5. The radial flow chromatography column of claim 1 , wherein any beads having r<0.225 R have been removed.6. The radial flow chromatography column of claim 1 , wherein the interstitial channels have: a tetrahedron site radius r=0.225 R claim 1 , an octahedron site radius r=0.414 R claim 1 , or both.7. The radial flow chromatography column of claim 1 , wherein the interstitial channels have a narrowest channel radius r=0.155 R.8. The radial flow chromatography column of claim 1 , wherein the beads are monodisperse.9. A process for selecting beads for use in a radial flow chromatography column comprising: a) ...

BEAD PACKING IN MICROFLUIDIC CHANNELS

A microfluidic bead-packing method includes activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel; packing the microbeads in the adsorbing channel; and activating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads. 1. A method comprising:activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel;packing the microbeads in the adsorbing channel; andactivating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads.2. The method of claim 1 , further comprising quantifying flow through the adsorbing channel.3. The method of claim 2 , wherein the activating the second micropump is based on determining that the quantified flow through the adsorbing channel is less than a predetermined threshold.4. The method of claim 3 , wherein the flow through the adsorbing channel is measured with a flow meter.5. The method of claim 1 , further comprising activating a microfluidic mixer located in the adsorbing channel.6. The method of claim 1 , further comprising activating an outlet nozzle to drain the sample fluid from the adsorbing channel.7. The method of claim 1 , wherein a bead clogging structure is located between the adsorbing channel and an outlet microchannel to pack the microbeads in the adsorbing channel.8. The method of claim 7 , wherein the bead clogging structure comprises a number of posts and gaps located between the adsorbing channel and the outlet microchannel to pack the ...

DYNAMIC AXIAL COMPRESSION FOR PREPARATIVE COLUMNS USING EXTERNAL COMPRESSION

A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly. 1. A dynamic axial compression column comprising: a first opening,', 'a second opening, and', 'a lumen extending from the first opening through the tube to the second opening;, 'a tube defininga first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening;a second end plate assembly sealing the second opening;a plurality of rods each extending along an outside of the tube and connecting the first end plate assembly and the second end plate assembly; anda first plurality of compression devices external to the tube, each of the first plurality of compression devices engaging one of the plurality of rods and biasing the first end plate assembly towards the second end plate assembly.2. The dynamic axial compression column of claim 1 , further comprising media filling the lumen.3. The dynamic axial compression column of claim 2 , wherein the media is compressible.4. The dynamic axial compression column of claim 2 , wherein the media is incompressible5. The dynamic axial compression column of claim 4 , wherein the media comprises at least one of:silica;alumina;zirconia;glass; ...

Method and Apparatus for Packing a Chromatography Column Assembly

A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube () having a closed first end () comprising an inlet/outlet (), a media inlet () adjacent a second end of the column tube and an adaptor () positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume. 1. A method for packing a chromatography column assembly with chromatography media , comprising the steps of:providing: a column tube having a closed first end comprising an inlet/outlet; a media inlet adjacent a second end of the column tube; and an adaptor positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume;connecting a media slurry source to the media inlet;at least partially filling the internal volume with media slurry via the media inlet; andforcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.2. The method according to claim 1 , wherein the adaptor comprises an inlet/outlet.3. The method according to claim 1 , further comprising providing the system and ...

METHOD FOR MANUFACTURING A GAS PHASE CHROMATOGRAPHY COLUMN AND COLUMN OBTAINED USING SUCH A METHOD

The present invention relates to a method for manufacturing a chromatography column, in particular for a gas phase chromatography, comprising a stationary phase made from a sol. 1. A method for manufacturing a chromatography column comprising a stationary phase made from a sol comprising a pore-forming agent , comprising:(a) introducing a sol comprising a pore-forming agent at a first end of the column,(b) moving said sol towards a second end of the column, so that a sol layer is formed on the internal wall of the column wherein said sol layer forms a gel on the internal wall of the column, and(c) drying the gel, and(d) removing the pore-forming agent from the dried layer, so as to form a microporous, mesoporous layer or other porous layer, the size and/or the density of the pores being controlled, said porous layer forming the stationary phase.2. The method according to claim 1 , wherein the sol forms a plug claim 1 , extending from the first end of the column and over a length of less than two-thirds of the total length of the column claim 1 , the plug being moved along the column under the effect of a pressure.3. The method according to claim 1 , wherein (c) is carried out by circulating a gas inside the column and claim 1 , if required claim 1 , during (a).4. The method according to claim 2 , wherein the gas is air or helium claim 2 , nitrogen or another inert gas.5. The method according to claim 1 , wherein the pore-forming agent comprises cetyltrimethylammonium bromide (CTAB) claim 1 , diblock copolymers of ethylene oxide and of propylene oxide claim 1 , triblock copolymers of ethylene oxide and of propylene oxide claim 1 , or another surfactant.6. The method according to claim 1 , wherein (d) is carried out after (c) with a treatment selected from the group consisting of calcination claim 1 , washing with an organic solvent of the alcohol or acetone type claim 1 , and UV insolation.7. The method according to claim 6 , wherein the calcination is carried out by ...

ENCAPSULATED PRE-ANALYTIC WORKFLOWS FOR FLOW-THROUGH DEVICES, LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRIC ANALYSIS

This invention relates to encapsulated workflow reagents comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same. 1. An encapsulated workflow reagent comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material.2. The encapsulated workflow reagent according to claim 1 , wherein the encapsulating material is attached to a surface of a scaffolding material.3. The encapsulated workflow reagent according to claim 1 , wherein the workflow reagent encapsulated within the encapsulating material is attached to or adsorbed onto the surface of a chromatographic material.4. The encapsulated workflow reagent according to claim 1 , wherein the encapsulating material is one or more polymers capable of providing a controlled release of the workflow reagents.5. The encapsulated workflow reagent according to claim 2 , wherein the scaffolding material is a solid claim 2 , a porous solid claim 2 , a non-porous solid claim 2 , a macroporous solid claim 2 , a mesoporous solid claim 2 , a microporous solid claim 2 , a nanoporous solid claim 2 , a superficially porous solid claim 2 , a perfusive solid claim 2 , a controlled pore solid claim 2 , an amorphous solid claim 2 , a radially aligned porous solid claim 2 , a non-radially aligned porous solid claim 2 , a circular ordered porous solid claim 2 , a crystalline solid claim 2 , a sintered solid claim 2 , a liquid claim 2 , a hydrogel claim 2 , an aerogel claim 2 , a xerogel claim 2 , a cryo-gel claim 2 , a soft-gel claim 2 , a gel-like material claim 2 , a wall-anchored monolith claim 2 , a wall-anchored polymeric high internal phase material claim 2 , a particle claim 2 , a monolith claim 2 , a membrane claim 2 , a poly-HIPE claim 2 , a mesh claim 2 , a fiber claim 2 , a screen claim 2 , an ...

Packing system and method for chromatography columns

The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.

CHROMATOGRAPHY COLUMN FRAME AND METHOD OF CONDUCTING MAINTENANCE ON AND PACKING OF A CHROMATOGRAPHY COLUMN

A chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder connected to said support arrangement. Said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction. 1. A chromatography column frame , comprising:at least two legs;a support arrangement connected to said at least two legs; anda holder means connected to said support arrangement,wherein said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction.2. The frame according to claim 1 , wherein the holder comprises at least one support plate provided with a recess for the adaptor rod.3. The frame according to claim 1 , wherein the at least one support plate is provided with a bearing surface for a driver arranged on the adaptor rod.4. The frame according to claim 3 , wherein the driver is a threaded sleeve arranged to interact with threads on the adaptor rod claim 3 , so that the adaptor rod moves in the vertical direction when pivoting the sleeve.5. The frame according to claim 2 , wherein two support plates are arranged on the support arrangement claim 2 , each plate is provided with a recess claim 2 , which recesses together define an opening for the adaptor rod.6. The frame according to claim 5 , wherein at least one of the support plates are removably connected to the support arrangement.7. The frame according to claim 1 , further comprising a stopper arranged on the support arrangement claim 1 , wherein the stopper is adapted to interact with an axial groove in the adaptor rod claim 1 , so that the adaptor rod is prevented from pivoting in said holder.8. The frame according to claim 1 , wherein the support arrangement ...

CONDITIONING OF PACKED CHROMATOGRAPHY COLUMNS

The present invention relates to a method of conditioning a dry-packed chromatography column, which method comprises providing a column packed with inert and hydrophilic chromatography resin; and driving a solvent or solvent mix across the column. The solvent(s) are selected to be less hydrophilic than the chromatography resin; and the rate of the solvent flow is controlled to a rate which does not cause the system pressure to exceed a predefined threshold. The present invention is advantageously used in FLASH chromatography, where the generation of heat may pose problems e.g. to plastic frits or filters at high flow rates and/or wetting of dry resins. 2. A method according to claim 1 , wherein the one or more solvents provide an equilibration of the chromatography resin.3. A method according to claim 1 , wherein the passing the one or more solvents includes passing two column volumes of solvent across the column.4. A method according to claim 1 , wherein the resin comprises porous silica.5. A method according to claim 1 , whereinthe packed column comprises at least one filter or frit andthe at least one filter or frit is arranged downstream of the chromatography resin in the column.6. A method according to claim 5 , wherein the at least one filter or frit includes a heat-sensitive material.7. A method of controlling heat generation in a column claim 5 , the method comprising:passing a solvent across the column at a flow rate, such that a column pressure does not exceed a threshold,wherein the resin is more hydrophilic than the resin.8. A method according to claim 1 , wherein the control is performed by a computer.9. A system for conditioning a dry-packed chromatography column claim 1 , the system comprising:a column packed with at least one inert and hydrophilic chromatography resin;at least one solvent container;means for measuring pressure within the system;means for passing a solvent at an actively controlled flow rate across the column; anda computer configured ...

Liquid chromatography packing material

A liquid chromatography packing material employing crosslinked polymer particles, wherein the volume average particle size of the crosslinked polymer particles is at least 2 μm but not more than 10 μm, the amount of fine particles having a particle size of 1 μm or less among the crosslinked polymer particles is less than 1% by volume, and the identity coefficient in the particle size distribution is not more than 1.15.

Column oven

A column oven includes a substantially sealed space surrounded by wall surfaces made of a heat conductive material to accommodate an analytical column, a heater for heating the wall surfaces made of the heat conductive material, and a heat insulating material surrounding an outside of the wall surfaces made of the heat conductive material. That is, a space inside the column oven in which the analytical column is accommodated is formed as a substantially sealed structure surrounded by the heat conductive wall surfaces, and the wall surfaces surrounding the substantially sealed space are heated by the heater, thereby heating the substantially sealed space uniformly from circumferential directions.

METHOD FOR ANALYZING RELATED SUBSTANCES OF A PHARMACEUTICAL COMPOSITION CONTAINING A POLYMERIC CARRIER

A method for analyzing related substances in a pharmaceutical composition containing an amphiphilic block copolymer comprising a hydrophilic block and a hydrophobic block as a polymeric drug carrier, related substances identified thereby, and a method for evaluating a pharmaceutical composition by using the same are provided. 1. A method for analyzing related substances of a polymeric micelle pharmaceutical composition , comprising:(1) preparing a sample for analysis of a polymeric micelle pharmaceutical composition comprising an amphiphilic block copolymer comprising a hydrophilic block (A) and a hydrophobic block (B), and a poorly water-soluble drug; and(2) analyzing the prepared sample by High Performance Liquid Chromatography (HPLC) using the following conditions (a) and (b):(a) a stationary phase of porous particles having a particle size of 4 μm or less; and(b) a column having an inner diameter of 5 mm or less and a length of 50 mm or more.2. The method for analyzing related substances of a polymeric micelle pharmaceutical composition according to claim 1 , wherein the stationary phase of porous particles is a pentafluorophenyl stationary phase.3. The method for analyzing related substances of a polymeric micelle pharmaceutical composition according to claim 1 , wherein the High Performance Liquid Chromatography is one using a stationary phase of porous particles having a particle size of 1.5 to 4 μm; and a column having an inner diameter of 2 to 5 mm and a length of 50 to 250 mm.4. The method for analyzing related substances of a polymeric micelle pharmaceutical composition according to claim 1 , further comprising qualitatively analyzing the related substances isolated in step (2) by LC/MS claim 1 , LC/MS/MS claim 1 , NMR or a combination thereof.5. The method for analyzing related substances of a polymeric micelle pharmaceutical composition according to claim 4 , wherein the LC/MS or LC/MS/MS is one using (c) a hybrid ODS stationary phase; and (d) a column ...

METHOD FOR AMINO ACID ANALYSIS AND SYSTEM FOR AMINO ACID ANALYSIS

The present invention provides a highly versatile method for amino acid analysis, the method enabling separation and analysis of amino acids in a sample with high precision in a shorter time. This method is a method for amino acid analysis, the method including a step of allowing a sample containing a plurality of amino acids to flow together with an eluent through a separation column packed with a cation exchange resin, thereby separating the amino acids, and a step of detecting the separated amino acids, wherein the eluent is an eluent containing a divalent or higher inorganic acid, a cation source, and water, and having a pH of 5.0 or lower, and the sample is allowed to flow through the separation column heated by applying a temperature gradient including a temperature region of 100° C. or higher. 1. A method for amino acid analysis , the method comprising:a step of allowing a sample containing a plurality of amino acids including a basic amino acid to flow together with an eluent through a separation column packed with a cation exchange resin, thereby separating the amino acids; anda step of detecting the separated amino acids, wherein:the eluent is an eluent containing a polybasic acid, a cation source, and water, and having a pH of 5.0 or lower;the cation source is an alkali metal salt, and an alkali metal constituting the alkali metal salt is at least any one of lithium (Li) and Na (sodium); andthe sample is allowed to flow through the separation column heated by applying a temperature gradient including a temperature region of 100° C. or higher.2. The method for amino acid analysis according to claim 1 , wherein the polybasic acid is at least one selected from the group consisting of sulfuric acid claim 1 , selenic acid claim 1 , phosphoric acid claim 1 , diphosphoric acid claim 1 , citric acid claim 1 , sulfosalicylic acid claim 1 , and fluorophthalic acid.3. The method for amino acid analysis according to claim 1 , wherein a highest temperature in the ...

USE OF VAPOR DEPOSITION COATED FLOW PATHS FOR IMPROVED ANALYTICAL ANALYSIS

A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I: 2. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of a polymeric material.3. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of glass.4. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of metal.5. The sample preparation device of claim 1 , wherein the alkylsilyl coating has a contact angle of at least 15°.6. The sample preparation device of claim 5 , wherein the alkylsilyl coating has a contact angle of less than or equal to 60°7. The sample preparation device of claim 1 , wherein the alkylsilyl coating has a thickness of at least 100 Å.8. The sample preparation device of claim 1 , wherein the alkylsilyl coating of Formula I is bis(trichlorosilyl)ethane or bis(trimethoxysilyl)ethane.10. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula II is (3-glycidyloxypropyl)trimethoxysilane claim 9 , n-decyltrichlorosilane claim 9 , trimethylchlorosilane claim 9 , trimethyldimethyaminosilane claim 9 , methoxy-polyethyleneoxy(1-10) propyl trichlorosilane claim 9 , or methoxy-polyethyleneoxy (1-10) propyl trimethoxysilane.11. The sample preparation device of claim 10 , wherein the alkylsilyl coating of Formula II is (3-glycidyloxypropyl)trimethoxysilane followed by hydrolysis.12. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula I and II provides a desired contact angle of about 5° to about 60°.13. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula I is bis(trichlorosilyl)ethane or bis ...

Method for Packing Chromatography Columns

The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns. 1. A method for packing a plurality of uniform chromatography columns , comprising the steps of:a) providing a plurality of chromatography columns;b) providing a plurality of chromatography resin aliquots;c) packing said chromatography resin aliquots in said chromatography columns to provide a plurality of packed chromatography columns; andd) subjecting said packed chromatography columns to repeated mechanical impacts.2. A method for packing a plurality of uniform chromatography columns , comprising the steps of:a) providing a plurality of chromatography columns;b) providing a plurality of chromatography resin aliquots;c) packing said chromatography resin aliquots in said chromatography columns to provide a plurality of packed chromatography columns; andd) subjecting said packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.3. The method of claim 1 , wherein said chromatography resin aliquots are dry and wherein in step c) said chromatography resin aliquots are filled in said chromatography columns and reswollen by adding a liquid to the chromatography columns.4. The method of claim 1 , wherein step b) comprises providing a plurality of dry chromatography resin aliquots and reswelling them with a liquid to provide a plurality of reswollen chromatography resin aliquots claim 1 , and wherein step c) comprises packing said reswollen chromatography resin aliquots in said chromatography columns.5. The method of ...

LIQUID CHROMATOGRAPHIC COLUMN AND LIQUID CHROMATOGRAPHIC APPARATUS INCLUDING THE SAME

A liquid chromatographic column includes: a cylindrical column body; an inflow-side filter that is disposed at an eluent inflow-side end of the column body; an outflow-side filter that is disposed at an eluent outflow-side end of the column body; and a filler that is filled between the inflow-side filter and the outflow-side filter, in which the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler, and the first resin filter member has an indentation elastic modulus lower than that of the second resin filter member. 1. A liquid chromatographic column , comprising:a cylindrical column body;an inflow-side filter that is disposed at an eluent inflow-side end of the column body;an outflow-side filter that is disposed at an eluent outflow-side end of the column body; anda filler that is filled between the inflow-side filter and the outflow-side filter,wherein the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler, andthe first resin filter member has an indentation elastic modulus lower than that of the second resin filter member.2. A liquid chromatographic column , comprising:a cylindrical column body;an inflow-side filter that is disposed at an eluent inflow-side end of the column body;an outflow-side filter that is disposed at an eluent outflow-side end of the column body; anda filler that is filled between the inflow-side filter and the outflow-side filter,wherein the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler,the first resin filter member is made of polyethylene, polypropylene, or polytetrafluoroethylene, andthe second resin filter member is made of polyether ether ketone or a ...

Laterally perfused chromatography element

A microfluidic chromatography element in which all components are situated in one plane and the mobile phase perfuses the stationary phase laterally.

Multi-capillary monolith made from amorphous silica and/or activated alumina

The invention relates to a monolithic porous material based on amorphous silica or activated alumina or on one of their mixtures, the material comprising substantially rectilinear capillary ducts that lie parallel to one another, and being intended to be used as packing in a chromatography column, characterised in that: the ducts have, relative to one another, a substantially uniform cross section; the cross-section of each duct is uniform over its entire length; the ducts pass right through the material; the volume of micropores smaller than 0.3 nm is smaller than 50% of the total porous volume of the material.

Systems and methods for failure mode detection in process chromatography

The disclosure provides systems and methods useful for predicting or detecting a malfunction in a chromatography process in real-time. In some embodiments, the disclosure provides systems and methods for detecting an atypical profile in a process chromatogram in ion-exchange chromatography of a biologic product.

COMPREHENSIVE 2DGC SYSTEM COMPRISING OF A MODULATION COLUMN, A MODULATOR, AND A GAS CHROMATOGRAPH

A comprehensive two-dimensional gas chromatography system comprising of a sample inlet, a primary dimension column, a secondary dimension column, a thermal modulator, and a detector. The thermal modulator has a first modulation position and a second modulation position. The sample inlet connects to the primary dimension column. The exit of the primary dimension column connects to the first modulation position via a fluidic path, the first modulation position connects to the second modulation position via a fluidic path, the second modulation position connects to the secondary dimension column via a fluidic path, and the exit of the secondary dimension column connects to the detector. The fluidic path between the exit of the primary dimension column and the inlet of the secondary dimension column is a modulation column. 1. A modulation column used in a comprehensive two-dimensional gas chromatography system: the modulation column has only part of its length coated with a stationary phase. The modulation column is either an independent part from the primary dimension column and the secondary dimension column , or an integral part of the primary dimension column or the secondary dimension column.2. The modulation column of claim 1 , has a first and a second modulation positions claim 1 , wherein (1) only the first and second modulation positions have stationary phase coating; or (2) only the first modulation position has stationary phase coating; or (3) only the first modulation position and a distance extending to its upstream has stationary phase coating; or (4) only the first modulation position claim 1 , the second modulation position claim 1 , and the length between these two positions have stationary phase coating; or (5) only the first modulation position claim 1 , the second modulation position claim 1 , the length between these two positions claim 1 , and a distance extending to upstream of the first modulation position have stationary phase coating; or (6) ...

CHROMATOGRAPHY COLUMN SUPPORT

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column. 2. The use according to claim 1 , characterized in that the legs are plates and that the connectors and the legs form a unit in which the upper edge of the respective leg is the connector.3. The use according to any one of the preceding claims claim 1 , characterized in that the legs are perforated plates.4. The use according to any one of the preceding claims claim 1 , characterized in that the support comprises one or more circular legs that have different radii with respect to each other and with respect to the axis of symmetry.5. The use according to any one of the preceding claims claim 1 , characterized in that a lateral flow between the sections defined by the legs of the support is possible.7. The support according to claim 6 , characterized in that the legs are plates and that the connectors and the legs form a unit in which the upper edge of the respective leg is the connector.8. The support according to any one of to claim 6 , characterized in that the legs are perforated plates.9. The support according to any one of to claim 6 , characterized in that the support comprises one or more circular legs that have different radii with respect to each other and with respect to the axis of symmetry.10. The support according to any one of to claim 6 , characterized in that a lateral flow between the sections defined by the legs of the support is possible.11. The ...

UNIFORMLY DENSE STATIONARY PHASE FOR CHROMATOGRAPHY

The present disclosure relates to a chromatographic stationary phase having a uniform polymer density, and related methods. In particular, the present disclosure relates to a method of forming a uniformly dense stationary phase inside a chromatography column. 1. A method of preparing a chromatography column , comprising(i) packing the chromatography column with support particles to form packed support particles within said column; and(ii) forming a polymer coating on the packed support particles.2. The method of claim 1 , wherein the support particles comprise silica claim 1 , alumina claim 1 , titania claim 1 , zirconia claim 1 , magnetite claim 1 , or combinations thereof.3. The method of claim 1 , wherein the support particles comprise an initiator for polymerization.4. The method of claim 1 , further comprising introducing a pre-polymer solution to the packed support particles.5. The method of claim 1 , wherein the step of forming a polymer coating comprises growing the polymer coating on the packed support particles.6. The method of claim 1 , wherein the step of farming a polymer comprises activators generated by electron transfer and atom-transfer radical polymerization.7. The method of claim 3 , wherein the step of forming a polymer comprises reversible addition fragmentation chain transfer claim 3 , and wherein the initiator is cyanomethyl [3-(trimethoxysilyl)propyl]trithiocarbonate.8. The method of claim 3 , wherein the step of forming a polymer atom-transfer radical polymerization claim 3 , and wherein the initiator is ((chloromethypphenylethyl)trimethoxysilane claim 3 , 3-trimethoxysilyl) propyl 2-bromo 2-methylpropionate claim 3 , [11-(2-bromo-2-methyl)propionyloxy]undecyltrichlorosilane or combinations thereof.9. The method of claim 1 , wherein the support particles comprise an initiator on a surface of the support claim 1 , and wherein the step of forming a polymer coating comprises growing the polymer coating on the surface of the packed support ...

Compositions, methods and microfluidics device for telomerase based in vitro diagnostic assays for detecting circulating tumor cells (ctc)

A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5′ and 3′ ITRs and a tumor-specific promoter; and (ii) a coding sequence for a reporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3′ ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample.

System and valve for liquid chromatography

Systems for use with liquid chromatography for provision of continuous flow or gradient flow in connection with two pumps providing mobile phase to a valve.

Chromatography Column Packing Medium Recovery

Systems include a chromatography column tube having an inlet and outlet and port assembly arranged in a wall of the column tube between flow distributors that together form a chamber within the tube that is filled with packing medium are described. The port assembly facilitates the removal of resin from the pre-packed column and the port assembly does not affect fluid flow in the normal use of the column for chromatographic separation or ability to maintain sanitary conditions within the column. Also described are methods that include attaching tubing to a pump and to a column inlet and a column outlet, opening the port assembly, attaching tubing to the port assembly and to a second reservoir, and pumping aqueous solution from a reservoir into the chamber and out through the port assembly into the second reservoir, thereby removing packing medium from the column along with the flowing aqueous solution. 1. A method of recovering packing medium from a pre-packed chromatography column comprising a column tube having an inlet and an outlet and a port assembly arranged in a wall of the column tube between first and second flow distributors that together form a chamber within the column tube that is filled with packing medium , the method comprising:attaching tubing from a first reservoir of aqueous solution to a pump and to the column inlet and the column outlet;opening the port assembly;attaching tubing to the port assembly and to a second reservoir; andpumping the aqueous solution from the first reservoir into the column inlet and column outlet into the chamber and out through the port assembly into the second reservoir, thereby removing packing medium from the column along with the flowing aqueous solution.2. The method of claim 1 , wherein opening the port assembly comprises removing a sanitary clamp from the port assembly.3. The method of any one of to claim 1 , wherein the second reservoir is the same as the first reservoir.4. The method of any one of to claim 1 , ...

SILICA-BASED STATIONARY PHASE FOR A CHROMATOGRAPHY COLUMN AND METHODS FOR PRODUCING THE SAME

A silica-based stationary phase for chromatography columns and the methods of preparing such. More particularly, but not by way of limitation, a silica-based stationary phase that is substantially free of polyethers (e.g., polymer glycols). Also, a chromatography column comprising a silica-based stationary phase substantially free of polyethers (e.g., polymer glycols) within its channels as either a thin-film coating and/or a monolith and/or a monolithic coating. More particularly, a micro-electro-mechanical system (MEMS) chromatograph comprising a silica-based monolith substantially free of polyethers (e.g., polymer glycols) as the stationary phase within the micro-channels of the column. 1. A method for the formation of a silica-based stationary phase for a chromatography column having an internal surface , wherein at least a portion of the internal surface is in contact with a polymerized sol-gel comprising reaction products of a silica precursor , a polyether , and a solvent , the method comprising:washing the chromatography column with an acid to substantially remove the polyether from the polymerized sol-gel, wherein the acid is at least one of an organic acid and a mineral acid, and further wherein the acid does not comprise hydrofluoric acid;washing the acid-washed chromatography column with a basic neutralization solvent; anddrying the neutralized chromatography column to form a silica-based stationary phase substantially free of the polyether.2. The method of claim 1 , wherein the polymerized sol-gel is formed by the method comprising:preparing a sol-gel solution comprising the silica precursor, the polyether, and the solvent;introducing the sol-gel solution into the chromatography column such that at least a portion of the internal surface of the chromatography column is in contact with the sol-gel solution; andheating the chromatography column to polymerize the sol-gel solution.3. The method of claim 1 , wherein the silica-based stationary phase ...

CONTAINERS FOR CHROMATOGRAPHY MEDIA

The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use. 1. A method of packing a chromatography column , said method comprising the steps ofa. inserting the flexible bag for chromatographic medium of comprising: an exterior wall of a non-porous material attached to both a first liquid distribution element and a second liquid distribution element at respective opposed ends of said exterior wall_thereby defining a compartment for chromatographic medium therein; said first liquid distribution element facing said second liquid distribution element, the first liquid distribution element being welded or moulded to the exterior wall; a medium filling port; and a first end piece and a second end piece attached to said exterior wall and adjacent to the first liquid distribution element and the second liquid distribution element respectively, the first end piece and the second end piece each comprising an opening for receipt of an inlet or an outlet for carrier liquid_and wherein the first liquid distribution element and the second liquid distribution element is each selected from the group consisting of filter, mesh, net, and sinter, and each of the first and second liquid distribution elements includes pores that are porous to liquids and do not allow the chromatographic medium to pass through into a chamber of a chromatography column;b. closing the column housing; andc. forming or compressing a packed bed or a consolidated bed or a fluidised bed of chromatographic medium.2. The method of claim 1 , wherein said bag contains dry chromatographic medium and said packed bed is formed by adding liquid to swell said medium to give a swollen volume Vs in a liquid of about 105-120% of the column chamber.3. The method of claim 1 , ...

LC-Column with Dynamic-Axial Compression (DAC)

A separating column for use with a filling tube to prepare for chromatography is disclosed. The separating column includes an axially movable spring stamp; and a plurality of springs coupled to the stamp. Moreover, the stamp is depressed directly into the column tube during column packing via the filling tube. Additionally, the stamp is fixed in the column tube while maintaining the packing pressure so that the springs are compressed during the packing process and press the stamp permanently and dynamically onto a chromatographic bed. 1. A separating column for use with a filling tube to prepare for chromatography , the separating column comprising:an axially movable spring stamp; anda plurality of springs coupled to the stamp,the stamp being depressed directly into the column tube during column packing via the filling tube; andthe stamp being fixed in the column tube while maintaining the packing pressure so that the springs are compressed during the packing process and press the stamp permanently and dynamically onto a chromatographic bed.2. The separating column according to claim 1 , wherein the plurality of springs hold the stamp in a fixed position inside the column tube.3. The separating column according to claim 1 , wherein the plurality of springs are comprised of stainless steel.4. The separating column according to claim 1 , further comprising a plurality suspension elements backing the stamp.5. The separating column according to claim 1 , wherein a DAC separating column is removed from the filling tube after a packing process.6. The separating column according to claim 2 , further comprising a plurality of spacers that comprise a plurality of half shells holding the spring stamp in the fixed position.7. A liquid chromatography column for use with a filling tube containing a particle-liquid mixture and a packing apparatus pressing the particle-liquid mixture from the filling tube into the liquid chromatography column claim 2 , the liquid chromatography ...

Hydrophilic anion exchange chromatography media

An anion exchange stationary phase comprises substrate particles, a based condensation polymer layer attached to the substrate particles, one or more alkylamine condensation polymer layers covalently attached to base condensation polymer layer, and a terminating condensation layer covalently attached to the alkylamine condensation polymer layer. They layers are formed from amines and polyethylene oxide where the hydroxyl groups are spaced from the amines by two carbon atoms.

Chromatography method

Method for exchanging materials, wherein a gaseous, liquid or supercritical mobile phase containing species to be separated is circulated through a packing comprising a stationary phase, the method being characterised in that:—the packing comprises a plurality of capillary ducts formed in at least one first material, the ducts passing through the packing between an upstream face through which the mobile phase enters the packing and a downstream face through which the mobile phase leaves the packing,—each duct comprises, on at least one portion of the inner wall thereof, at least one secondary material consisting of an organic gel or porous mineral,—the thickness of the secondary material defines, inside the duct, at least one empty tubular channel of solid material, the channel being open so as to allow the mobile phase to enter and extending continuously between the upstream and downstream faces of the capillary duct—the secondary material has a thickness between 0.05 times and 0.5 times the diameter of the channel—the method is carried out with a velocity of the mobile phase between 5.0 times and 50 times the speed of the optimum mobile phase defined by the minimum of the Van Deemter curve of the majority separating compound under the method conditions—the cumulative volume of the capillary ducts is more than 15% of the total packing volume.

METHOD OF AND DEVICE FOR PACKING A CHROMATOGRAPHY COLUMN

A method and device for packing a chromatography column formed of one or more vibration devices attached to top and/or bottom flanges of the column. Media is added in one or more steps to the column, allowed to settle under the effects of gravity and then subjected to one or more treatments of vibration from the vibration devices until a suitably packed column is obtained. Liquid used to suspend the media while being placed into the column may be at least partially removed before or during the vibration step(s). The remaining liquid is then removed or replaced after the packing has been obtained. 1. A method of packing a chromatography column comprising the steps of:a. providing a chromatography column having one or more vibration devices attached to its outer surface;b. forming a slurry with a media and a buffer solution;c. transferring the slurry into the column and immediately applying flow for 1 minute to settle the slurry in the column;d. activating the one or more vibration devices to generate vibrations for 1 minute following the settling of the slurry under flow for 1 minute;e. repeating flow for 1 minute followed by vibrations for 1 minute, until a packed bed of media is obtained.2. The method of claim 1 , wherein the media comprises controlled pore glass with a Protein A ligand.3. The method of claim 1 , wherein the buffer solution is present from about 10 to about 90% of the volume of the slurry.4. The method of claim 2 , wherein the controlled pore glass media has a 100 μm diameter and pore size of 1000A.5. The method of claim 1 , wherein the column comprises two or more vibration devices on the outer surface of the column.6. The method of claim 5 , wherein the two or more vibration devices are evenly spaced apart on the outer surface of the column.7. The method of claim 1 , wherein the column comprises three or more vibration devices on the outer surface of the column.8. The method of claim 7 , wherein the three or more vibration devices are evenly ...

Chromatography column qualification in manufacturing methods for producing anti-il12/il23 antibody compositions

A method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA® (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

CHROMATOGRAPHY COLUMN QUALIFICATION IN MANUFACTURING METHODS FOR PRODUCING ANTI-TNF ANTIBODY COMPOSITIONS

A method of operating a chromatography column is described for use in methods of manufacture for producing anti-TNF antibodies, e.g., the anti-TNFα antibody SIMPONI® (golimumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value. 2. The method of claim 1 , further comprising:conditioning, replacing, or repacking the chromatography column based on said assessing.3. The method of further comprising:collecting column outlet signal and accumulated flow parameters at two or more intervals of a corresponding mobile phase transition front during one or more subsequent uses of the chromatography column packing;performing said determining and said calculating using the column outlet signal and accumulated flow parameters collected during each of the one or more subsequent uses of the chromatography column packing;determining an HETP value of the chromatography column packing during each of said one or more subsequent uses based on said performing;compiling a trend of the determined HETP values of the chromatography column packing of the two or more subsequent uses; andidentifying a change in the quality of the chromatography column packing based on said compiled trend, wherein said conditioning, replacing or repacking the chromatography column is based on said identifying.4. The method of claim 3 , wherein an increase in the HETP value of ...

Method of Separating Ions

The present disclosure relates generally to a method of separating ions according to their ion mobility, comprising (i) accumulating a first population of ions in a first region of an ion mobility separator, (ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator, and (iii) accumulating a second population of ions in said first region of said ion mobility separator whilst said first population of ions are being separated according to their ion mobility in said ion mobility separator. 1. A method of separating ions according to their ion mobility , comprising:(i) accumulating a first population of ions in a first region of an ion mobility separator;(ii) separating said first population of ions according to their ion mobility in said first region of said ion mobility separator; and(iii) accumulating a second population of ions in said first region of said ion mobility separator whilst said first population of ions are being separated according to their ion mobility in said ion mobility separator.2. A method as claimed in claim 1 , wherein step (ii) comprises accumulating said second population of ions in an accumulation region upstream of said first region claim 1 , whilst said first population of ions are being separated according to their ion mobility in said first region of said ion mobility separator.3. A method as claimed in claim 2 , wherein step (iii) comprises transferring said second population of ions from said upstream accumulation region and into said first region claim 2 , and continuing to pass ions through said upstream accumulation region and into said first region so as to accumulate said second population of ions in said first region.4. A method as claimed in claim 1 , wherein said ion mobility separator comprises a second region downstream of said first region claim 1 , and step (ii) comprises separating said first population of ions according to their ion mobility in said ...

GC-FTIR and Mode of Operation to Address Water Interference

Samples are analyzed in a system that includes a gas chromatography column for separating components in a sample and a spectrometry system for detecting these components. An interferent present in the sample, water for example, flows through the column and the sample cell of the spectrometry system before beginning the analysis of analytes. 1. An analysis method comprising:allowing a fluid interferent from an interferent-containing sample to flow through a gas chromatography column and a sample cell of a spectrometry system before beginning an analysis of analytes.2. The method of claim 1 , wherein a flow rate of another carrier fluid added to the fluid interferent is within the range of from 0 mL/min to about 0.5 mL/min.3. The method of claim 1 , wherein another carrier gas is added to the fluid interferent at a flow rate sufficient to prevent back flow of the fluid interferent claim 1 ,4. The method of claim 1 , wherein a flow rate of another carrier fluid added to the fluid interferent is no greater than about 5% relative to the flow rate of the fluid interferent.5. The method of claim 1 , wherein the interferent is water and the analytes include at least one non-polar volatile organic compound.6. The method of claim 5 , wherein the at least one non-polar volatile organic compound is benzene or toluene.7. The method of claim 1 , wherein the gas chromatography column has a length claim 1 , an inner diameter and/or a coating thickness selected to enhance complete elution of the interferent before elution of the analyte.8. The method of claim 1 , wherein a length of the gas chromatography column is not greater than about 30 meters.9. The method of claim 1 , wherein an inner diameter of the gas chromatography column is equal to or greater than about 0.53 mm.10. The method of claim 1 , wherein a coating on the gas chromatography column is non-polar and/or has a thickness of at least about 3.0 microns.11. The method of claim 1 , further comprising purging the sample ...

Thermally Modified Polymeric Organosilicon Material, Method for Preparing Said Material and the Uses Thereof

Materials obtained by thermal modification of polymeric organosilicons such as polydimethylsiloxane (PDMS) or derivatives of PDMS. Methods of preparing said materials and uses of said materials. 1. A material that is a modified PDMS or derivative of PDMS prepared by thermal treatment of a column containing PDMS or a derivative of PDMS at a temperature in the range of about 400° C. to about 1000° C.2. The material according to wherein the column is a capillary column.3. The material according to wherein the capillary column has an outer tube comprising metal or fused silica claim 2 , and an inner coating of PDMS or a derivative of PDMS.4. The material according to wherein the thermal treatment takes place in the presence of oxygen.5. The material according to wherein a flow of an oxygen containing gas is passed through the column during the thermal treatment.6. The material according to wherein the modified PDMS or derivative of PDMS is in the form of nanoparticles.7. The material according to wherein the thermal treatment comprises heating the column to a temperature in the range of 400° C. to 1000° C. for 1 to 5 heating cycles of 2-15 minutes per cycle.8. The material according to wherein the thermal treatment comprises heating the column to a temperature in the range of 750° C. to 850° C. for 1 cycle of 10 minutes followed by 2 cycles of 5 minutes.9. A column containing a modified PDMS material or a modified PDMS derivative material prepared by thermal treatment of a column containing PDMS or a derivative of PDMS at a temperature in the range of about 400° C. to about 1000° C.10. The column according to wherein the column is a capillary column having an outer tube comprising metal or fused silica and an inner coating of PDMS or a derivative of PDMS.11. The column according to wherein the thermal treatment process takes place in the presence of oxygen.12. The column according to wherein a flow of an oxygen containing gas is passed through the column during thermal ...

MONOLITHIC SORBENTS HAVING A METAL CLADDING

The invention relates to monolithic sorbents which are clad with tubes made of metal. The metal cladding can be applied directly to the monolithic sorbents by cold forming. This enables very mechanically stable cladding of the monolithic sorbents with minimal dead space. 1. Process for the production of chromatography columns containing a monolithic sorbent which has been clad with a metal tube , bya) provision of a cylindrical monolithic sorbent and a metal tube having an internal diameter which is larger than the diameter of the monolithic sorbentb) introduction of the monolithic sorbent into the metal tubec) application of the metal tube to the monolithic sorbent by cold forming.2. Process according to claim 1 , characterised in that the metal tube is a stainless-steel tube.3. Process according to claim 1 , characterised in that the cold forming in step c) is carried out by rotary swaging.4. Process according to claim 3 , characterised in that the rotary swaging in step c) is carried out using a hammering tool having two hammering jaws and the speed of rotation is between 500 minand 1500 min.5. Process according to claim 1 , characterised in that claim 1 , in step a) claim 1 , the internal diameter of the metal tube is 0.1 to 0.25 mm greater than the external diameter of the monolithic sorbent.6. Process according to claim 1 , characterised in that the monolithic sorbent consists of silica gel or inorganic/organic hybrid materials.7. Process according to claim 1 , characterised in that a monolithic sorbent having a length of 1 cm to 30 cm and a diameter of 1 mm to 5 cm is provided in step a).8. Process according to claim 1 , characterised in that the monolithic sorbent clad with the metal tube is provided in an additional step d) with connections for solvent feed and discharge.9. Process according to claim 1 , characterised in that the monolithic sorbent provided in step a) has been derivatised with separation effectors.10. Chromatography column having a ...

Spin coated stationary phase microfabricated gas chromatographic columns

Presented herein is a new concept of uniformly spin coating a flat surface with a stationary phase and creating a gas chromatography column by pressing a grooved lid, with micro-stamped ridges, down onto the coated substrate. The lids are molded out of commercially available rigid materials including epoxies so that when pressed onto a flat surface it will create an air tight seal. The epoxy material is rendered inert by a thin layer of gold.

PACKAGE FOR BATCH CHROMATOGRAPHY

A method for separating at least one target compound from a feed solution is provided. The method includes filling a bioprocess package with a chromatography resin. The bioprocess package includes a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet. The method further includes flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin, washing the chromatography resin in the bioprocess package, and eluting the chromatography resin. 1. A method for separating at least one target compound from a feed solution , the method comprising: a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container; and', 'a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet;, 'filling a bioprocess package with a chromatography resin, the bioprocess package comprisingflowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin;washing the chromatography resin in the bioprocess package; andeluting the chromatography resin such that substantially all of the at least one target compound is released from the chromatography resin.2. The method of claim 1 , wherein filling a bioprocess ...

STACKABLE PLANAR ADSORPTIVE DEVICES

Adsorptive bed devices include a monolithic scaffold having a stress absorbing rigid structure and open cells filled with adsorptive beads. The monolithic scaffold restricts movement of the plurality of adsorptive beads, absorbs stress induced by a hydraulic pressure gradient along a direction of liquid flow. In one embodiment the adsorptive bed is packed into a chromatography column, and in another embodiment the adsorptive bed is sealed in a monolithic block. In another embodiment, the adsorptive bed device includes an adsorptive block, first and second planar distributors and peripheral seal. 1. An adsorptive bed to receive a liquid flow comprising:a monolithic scaffold including a structural shell;an interconnected lattice disposed within the structural shell; a plurality of vertical plates; and', 'a plurality of horizontal plates intersecting the plurality of vertical plates;, 'wherein the interconnected lattice comprises an array of channels comprisinga plurality of adsorptive beads filling the array of channels forming a packed bed of the plurality of adsorptive beads; andwherein the monolithic scaffold restricts movement of the plurality of adsorptive beads, absorbs tensile stress induced by a hydraulic pressure within the adsorptive bed.2. The adsorptive bed of claim 1 , wherein the plurality of vertical plates and the plurality of horizontal plates are fused together to form the interconnected lattice as the monolithic scaffold by virtue of an extrusion process.3. The adsorptive bed of claim 2 , wherein a cross-section of each of the channels of the array of channels is one of:a regular cross-section;an irregular cross-section;a circular cross-section;a triangular cross-section;a rectangular cross-section; andan hexagonal cross-section.4. The adsorptive bed of claim 2 , wherein the monolithic scaffold comprises one of:plastic;metal; andceramic.5. The adsorptive bed of claim 2 , wherein the further comprises:a top planar surface;a bottom planar surface;a ...

Porous silica particles

The present disclosure provides a porous silica having an average pore diameter of from 20 to 450 Å, a median (D50) pore diameter of from 20 to 450 Å, a pore volume of from 0.15 to 1.2 cm 3 g −1 , a surface area of from 100 to 600 m 2 g −1 , and a span of 0.80 or less. The present disclosure also provides a method of producing the porous silica. The method includes the step of mixing together an aqueous phase comprising nanoparticulate silica and an organic phase to form a water-in-oil dispersion or emulsion. The organic phase includes an organic solvent that is insoluble or partially soluble in water and optionally also includes a non-polar organic compound that is insoluble in water and at least partially soluble in the organic solvent. A gelling agent is present in the aqueous phase such that the nanoparticulate silica gels form the porous silica.

COLUMN, COLUMN FILLING APPARATUS, COLUMN FILLING SYSTEM, AND COLUMN PROCESSING METHOD

A column filling apparatus mainly includes a tank, a stirrer being an agitator, a pump, a resin bottle, first to eleventh pipes, first to fourth valves composed of three-way valves, and fifth and sixth valves composed of two-way valves. A suction port is mounted on a leading end of the fifth pipe, and the suction port is inserted into an inner part from a top opening of the resin bottle. The other end of the fifth pipe is connected to the first valve. The first valve is connected to the first pipe, the second pipe, and the fifth pipe, and connects or disconnects these pipes to or from each other. An end of the first pipe is mounted near a bottom of the tank, and is opened near an inner bottom surface of the tank. 1. A column filling apparatus comprising:a tank storing liquid and a filler;a transfer pipe transferring slurry from the tank to a column, the slurry comprising the liquid and the filler; anda reflux pipe for refluxing the slurry from the column to the tank.2. The column filling apparatus according to claim 1 , wherein the transfer pipe allows the slurry to flow into the column from an upper side with respect to a filling completion position of the filler in the column.3. The column filling apparatus according to claim 1 , wherein the reflux pipe takes out the slurry from the column from an upper side with respect to a filling completion position of the filler in the column.4. The column filling apparatus according to claim 1 , wherein the transfer pipe takes out the slurry from a vicinity of a bottom of the tank.5. The column filling apparatus according to claim 1 , wherein the transfer pipe includes a pump that transfers the slurry from the tank to the column.6. The column filling apparatus according to claim 1 , further comprising an agitator that is provided in the tank claim 1 , and mixes the liquid and the filler.7. The column filling apparatus according to claim 1 , further comprising:a container storing the liquid and the filler;a container take-out ...

Chromatography Column Comprising an Internal Bracing

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber. 1. A chromatography column for bioprocess separations , said column comprising:a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen;b) a first end wall, secured to or integral with said at least one side wall, with a first port fluidically connected via a first distributor to said first bed support screen;c) a second end wall, secured to or integral with said at least one side wall, with a second port fluidically connected via a second distributor to said second bed support screen;d) a packing port in one of said at least one side wall and first and second end walls; ande) an internal bracing secured to, or integral with, at least one of said first and second end walls and extending into said bed chamber.2. The chromatography column of claim 1 , wherein at least part of said internal bracing spans the entire axial height of the bed chamber claim 1 , being secured to claim 1 , or integral with claim 1 , both of the first and second end walls.3. The chromatography column of claim 1 , wherein said internal bracing comprises one or more rods claim 1 , each having a first rod end secured to claim 1 , or integral with claim 1 , said first end wall and a second rod end secured to claim 1 , or integral with claim 1 , said second end wall.4. The ...

Selection and design of columns for liquid chromatography

The present disclosure is directed at the selection and design of columns for liquid chromatography including liquid chromatography devices and systems and corresponding methods of operation, particularly in the field of high pressure liquid chromatography (HPLC).

Membrane adsorber device

The present invention is an adsorber membrane and a device containing one or more such membranes. Both the membrane and the device have a Peclet number (Pe) of at least 100. The membrane and the device are designed for the removal of trace contaminants in protein containing streams such as exist in the biopharmaceutical industry. A preferred membrane has tight pore size distribution and high permeabilities that allow for high throughput separations. A device of the present invention can contain a flat sheet membrane such as a pleated filter, a tangential flow filter or a spiral wound filter. Preferably, the device is formed in a stacked disk arrangement where one or more layers of membrane are sealed to each of the two large surface of the disk. One such device is formed of a series of disks, each disk having eight layers of membranes sealed to each of the two large surfaces of the disk. These disks are placed within a sealed capsule having an inlet on one end and an outlet on the other. The disks are sealed so that all fluid that exits the outlet does so by having first passed through the membranes on one side of a disk.

Suppression of electroosmosis with hydrolytically stable coatings

Surfaces of silica-containing materials, such as the inner walls of silica capillaries, used in chromatographic, particularly electrophoretic, separations are coated with an organic polymer layer to reduce or eliminate surface charges. The layer is applied by first converting the silanol groups on the surface to silicon halide groups, then reacting these groups with an organometallic reagent having a terminal ethenyl moiety, preferably vinyl or allyl lithium or a vinyl or allyl magnesium halide, to convert the silicon halide groups to Si--R groups where the R retains the terminal ethenyl moiety, and finally reacting these ethenyl groups newly attached to the surface with a neutral organic monomer in an addition polymerization reaction to form a monomolecular noncrosslinked polymer layer over the surface. The resulting polymer layer is linked to the silica directly through a Si--C bond which is stable over a wide range of pH conditions.

Measurement of unsaturation level in butyl and EPDM rubbers

A method is provided for quantitatively determining the amount of unsaturation in butyl and EPDM rubbers using a gel permeation chromatograph in combination with an ultraviolet detector. Placement of a differential refractive index detector in series with said ultraviolet detector provides a method for the simultaneous measurement of the amount of unsaturation, the molecular weight distribution and the amount of antioxidant in said rubbers.

Metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same

Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.

Methods for coating metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same

Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.

CHROMATOGRAPHIC CASSETTE

A chromatographic cassette includes a cassette including a chamber, chromatographic media disposed within the cassette chamber, a distribution network fluidly coupled to the chromatographic media and an inlet port and an outlet port coupled to the distribution network. A hyper-productive chromatography technique includes providing a scalable and stackable chromatographic cassette, loading a sample to be processed, operating the scalable chromatographic cassette having an adsorptive chromatographic bed having a volume greater than 0.5 liter by establishing a flow at a linear velocity greater than 500 cm/hr with a residence time of the loading step of less than one minute.

Capillary column chromatography process and system

A microfluidic system is provided for separating components of many fluid samples in a parallel fashion through an array of capillary conduits. The system includes capillary conduits for receiving fluid samples, and for effecting separation of substituents of the sample by passing the sample through a corresponding array of capillary separation conduits containing a solid separation medium. The microfluidic circuit is made of three or more substrate layers and preferably includes etched channels in different layers that are dimensioned relative to one another to provide for retaining the solid composition within the capillary separation conduits. The system also includes an array of detector flow cells in fluid communication with the capillary separation conduits, and an array of high pressure connectors for connecting discrete capillary tubes to the fluid passages in the microfluidic circuit for introducing and removing the fluid samples from the system. The connections for the capillary tubes have a small footprint, are readily reversible (allowing ease of maintenance and replacement), and can seal against high pressures.

Capillary column chromatography process and system

A microfluidic system is provided for separating components of many fluid samples in a parallel fashion through an array of capillary conduits. The system includes capillary conduits for receiving fluid samples, and for effecting separation of substituents of the sample by passing the sample through a corresponding array of capillary separation conduits containing a solid separation medium. The microfluidic circuit is made of three or more substrate layers and preferably includes etched channels in different layers that are dimensioned relative to one another to provide for retaining the solid composition within the capillary separation conduits. The system also includes an array of detector flow cells in fluid communication with the capillary separation conduits, and an array of high pressure connectors for connecting discrete capillary tubes to the fluid passages in the microfluidic circuit for introducing and removing the fluid samples from the system. The connections for the capillary tubes have a small footprint, are readily reversible (allowing ease of maintenance and replacement), and can seal against high pressures.

Method for producing a chromatography column

Molecular topological frationation of macromolecules

A process for characterizing a sample comprising a population of linear macromolecules of interest (104) and a population of long chain branched macromolecules of interest (103), the process including four steps. The first step is to provide a flow through separating medium (100) and a liquid eluant (101) in which the macromolecules of interest dissolve, the separating medium defining flow through channels (102), the eluant flow rate and the average diameter of the channels being in a range so that the linear macromolecules of interest elute before the long chain branched macromolecules of interest (105) (106). The second step is to introduce a sample into the liquid eluant. The third step is to flow the liquid eluant under pressure through the channels (102) of the separating medium (100). The fourth step is to differentiate the linear macromolecules of interest (104) from the long chain branched macromolecules of interest (103) based on their successive elution volumes established in the third step such as by determining the refractive index of the successive elution volumes or by subjecting the successive elution volumes to size exclusion chromatography.

Column for analytical temperature rising elution fractionation (atref)

The present invention relates to a column for performing analytical temperature rising elution fractionation (ATREF) characterized in that the packing of said column comprises elastic wires having a good elasticity and a good thermal conductivity. The present invention also relates to a device comprising the column according to the invention, a temperature controlling system for controlling the temperature of said column, a sample injector for injecting a polymer sample solution into said column, a pump for eluting the polymer fractions from said column and a detector for detecting eluting fractions of said sample solution. The present invention further relates to a method for performing ATREF analyses using the column according to the invention.

Column for analytical temperature rising elution fractionation (atref)

The present invention relates to a column for performing analytical temperature rising elution fractionation (ATREF) characterized in that the packing of said column comprises elastic wires having a good elasticity and a good thermal conductivity. The present invention also relates to a device comprising the column according to the invention, a temperature controlling system for controlling the temperature of said column, a sample injector for injecting a polymer sample solution into said column, a pump for eluting the polymer fractions from said column and a detector for detecting eluting fractions of said sample solution. The present invention further relates to a method for performing ATREF analyses using the column according to the invention.

Column for analytical temperature rising elution fractionation (ATREF)

The present invention relates to a column for performing analytical temperature rising elution fractionation (ATREF) characterized in that the packing of said column comprises elastic wires having a good elasticity and a good thermal conductivity. The present invention also relates to a device comprising the column according to the invention, a temperature controlling system for controlling the temperature of said column, a sample injector for injecting a polymer sample solution into said column, a pump for eluting the polymer fractions from said column and a detector for detecting eluting fractions of said sample solution. The present invention further relates to a method for performing ATREF analyses using the column according to the invention.

将可收缩支撑料用于多孔整体材料的方法和设备

本发明提供了一种加工制品和装置及其形成和使用方法，其中所述制品包括容纳在无机材料制成的支承体中或由该支承体限制边界的多孔无机基材，其中所述多孔基材和支承体被加热到一定温度，使支承体有效地收缩到多孔基材上，从而在多孔基材与支承体之间形成液密封接触。在一个优选方面，多孔无机基材的总孔隙率至少为5％，并且是用溶胶－凝胶法形成的多孔整体料。这样形成的制品提供了通过多孔基材的受限流体流，在分离、催化、过滤等方面提供了优异的性能。

Method and apparatus for forming an homogeneous mixture of chromatography media in a vessel

The present invention relates to methods and apparatus for forming an homogeneous mixture of chromatography media in a vessel. The invention also relates to methods and apparatus for transferring the homogeneous media from the vessel into a second vessel in preparation for packing a chromatography column. The invention can also be used directly to pack the column with homogeneous media.

分离装置,分离方法和生产分离装置的方法

本发明提供了在短时间内利用少量样品以优异分辨率分离样品的分离技术，所述技术很少引起诸如阻塞的问题。多个疏水区705以大约等间距排列在样品通过的槽路中，而且亲水基材701的表面暴露在除疏水区705以外的区域。

ガスクロマトグラフ用キヤピラリ−カラム

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Nucleophilic bodies bonded to siloxane and use thereof for separations from sample matrices

A structure comprising bodies having a functional surface property bonded to a substrate via a siloxane polymer adhesive. The structure may comprise a novel composition comprising a siloxane polymer having carbon bodies bonded thereto by direct carbon to silicon bonds. This composition may be used to bond carbon particles through a medium comprising the siloxane polymer to a vitreous, metal, plastic or other nucleophilic substrate. Alternatively, the bodies may comprise alumina, silicon, zeolite, organic polymers or other nucleophilic compositions, which are bonded directly to silicon atoms of the siloxane polymer. To bond carbon or other nucleophilic bodies to the substrate, the substrate is contacted with a mixture of the bodies and a hydrosiloxane polymer. The mixture is heated to cause the polymer to be bonded to the nucleophilic bodies, typically by C--Si, C--O--Si, Si--O--Si or Si--O--Al bonds, and to the substrate by reaction with the surface silanol or other nucleophilic groups. Chromatographic methods using a column comprising the novel composition are also disclosed.

Zeolitic capillary columns for gas chromatography

Methods have been found to prepare fused silica capillary gas chromatographic columns where the stationary phase is a molecular sieve affixed to the silica capillary wall without the aid of an organic binder by modifying the interior surface of the silica prior to contact with the molecular sieve. These totally inorganic columns greatly expand the application and range of gas chromatographic separations and allow the use of non-traditional carrier gases, even air, while not degrading the separation of components. The columns are films of small molecular sieve particles affixed to a silica surface modified by such treatments as hydrogen peroxide, alumina deposition, or silica deposition followed by fixation at 80°-160° C.