21-03-2013 дата публикации
Номер: US20130071940A1
Chemoselective derivatization of biological amines, carboxylic acids, aldehydes or ketones are employed in methods to detect a plurality of components, or members of a component, such as metabolites, that vary in molecular structure. The methods of the invention can be employed in aqueous and nonaqueous conditions. 1. A method of identifying components in a biological sample , comprising the steps of: i) an amino acid or an amine,', 'ii) an aldehyde or a ketone, or', 'iii) a carboxylic acid or an alcohol, with an isocyanide and Ugi reactants that consists essentially of two of i), ii) and iii) apart from the component, thereby derivatizing at least a portion of that component; and, 'a) combining at least a portion of a biological sample, the biological sample including at least one component that is, or includes,'}b) identifying the derivatized component of the biological sample.2. The method of claim 1 , wherein the component is a first component claim 1 , and wherein the biological sample includes a second component that is claim 1 , or includes claim 1 ,i) an amino acid or an amine,ii) an aldehyde or a ketone, oriii) a carboxylic acid or an alcohol, that is not any of i), ii) or iii) of the first component, and further including the steps ofa) combining at least a portion of the remainder of the biological sample with an isocyanide and Ugi reactants that consists essentially of two of i), ii) and iii) apart from the second component, thereby derivatizing at least a portion of the second component; andb) identifying the derivatized second component.3. The method of claim 1 , wherein the isocyanide is labeled claim 1 , isotopic or ionic.4. The method of claim 1 , wherein the component is selected from the group consisting of proteins claim 1 , polyamines claim 1 , fatty acids claim 1 , neurotransmitters claim 1 , hormones claim 1 , α-amino acids claim 1 , intermediates in central metabolism claim 1 , antibiotics claim 1 , carbohydrates claim 1 , co-factors and co- ...
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