POLYMER DOT COMPOSITIONS AND RELATED METHODS

Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

METHODS OF DETERMINING RELATIVE POTENCY OF GLATIRAMER ACETATE AND USE THEREOF IN PREPARING A BATCH OF GLATIRAMER ACETATE AS ACCEPTABLE FOR PHARMACEUTICAL USE

A method of determining relative potency of glatiramer acetate (GA) is provided. Accordingly there is provided a method comprising: immunizing a mammal with Poly-YAK copolymer (pYAK); preparing a primary culture of T-cells from the immunized mammal; stimulating samples of the primary culture of T-cells with a reference standard batch of GA or a batch of GA; determining a stimulation parameter of the cells in each sample following a predetermined incubation time; and comparing the stimulation parameter in the cells so as to determine the relative potency of the batch of GA. Also provided are methods of preparing a batch of GA as acceptable for pharmaceutical use and treating multiple sclerosis (MS) in a subject. 1. A method of determining relative potency of a batch of glatiramer acetate (GA) , the method comprising:(a) immunizing a mammal with an immunization effective amount of a Poly-YAK copolymer (pYAK) so as to obtain an immunized mammal;(b) preparing a primary culture of T-cells from said immunized mammal;(c) individually stimulating samples comprising substantially identical number of cells of said primary culture of T-cells with a predetermined amount of:(i) a reference standard (RS) batch of GA; or(ii) the batch of GA,wherein said predetermined amount of (i) is substantially identical to said predetermined amount of (ii);(d) determining a stimulation parameter of said cells in each said samples following a predetermined incubation time; and(e) comparing said stimulation parameter in said cells of step (c)(i) with said stimulation parameter of said cells of step (c)(ii), so as to determine the relative potency of the batch of GA.2. The method of claim 1 , further comprising generating stimulation curves of said samples incubated with predetermined amount of said RS batch of GA and of said samples incubated with predetermined amount of said batch of GA claim 1 , so as to determine parallelism.3. The method of claim 1 , wherein said mammal is a rodent.46-. ( ...

CATIONIC POLYMER SYSTEMS FOR SELECTIVE BACTERIAL CAPTURE

A device and methods of use thereof for isolating one or more microorganisms from a biological sample, the device comprising a polymeric surface having one or more cationic polymers covalently grafted thereto, wherein the one or more cationic polymers have a selective affinity for the one or more microorganisms. 1. A device for isolating one or more microorganisms from a biological sample , the device comprising a polymeric surface having one or more cationic polymers covalently grafted thereto , wherein the one or more cationic polymers have a selective affinity for the one or more microorganisms.2. The device of claim 1 , wherein the polymeric surface is hydrophobic.3. The device of claim 1 , wherein the cationic polymer comprises poly-diallyldimethyl ammonium chloride (pDADMAC).4. The device of claim 1 , wherein the polymeric surface comprises a polymer selected from the group consisting of poly(ethylene terephthalate) (PET) claim 1 , polystyrene (PSt) claim 1 , polyethylene (PE) claim 1 , poly(methyl methacrylate) (PMMA) claim 1 , or a combination thereof.5. The device of claim 1 , wherein the polymeric surface comprises a cationic monomer that has undergone UV-initiated polymerization to form a cationic-grafted polymeric surface.6. The device of claim 1 , further comprising a receptacle configured to receive the biological sample.7. The device of claim 1 , further comprising a microscope.8. The device of claim 7 , wherein the microscope is selected from the group consisting of a light microscope and a fluorescence microscope.9. The device of claim 8 , wherein the fluorescence microscope is a light-emitting diode (LED) microscope.10. A method for isolating one or more microorganisms from a biological sample claim 8 , the method comprising:(a) providing a device comprising a polymeric surface having one or more cationic polymers covalently grafted thereto, wherein the one or more cationic polymers have a selective affinity for the one or more microorganisms;(b) ...

Diagnostic methods for the detection and quantification of blood-related diseases

A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field.

POLYMER DOT COMPOSITIONS AND RELATED METHODS

Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions. 1126-. (canceled)127. A lyophilized composition comprising:a lyophilization agent; anda plurality of fluorescent nanoparticles,wherein fluorescent nanoparticles of the plurality of fluorescent nanoparticles comprises at least one condensed conjugated, semiconducting polymer; andwherein the lyophilization agent is present between about 1% w/v and 50% w/v.128. The lyophilized composition of claim 127 , wherein the lyophilization agent comprises a carbohydrate129. The lyophilized composition of claim 128 , wherein the carbohydrate is selected from the group consisting of a monosaccharide claim 128 , a disaccharide claim 128 , an oligosaccharide claim 128 , a polysaccharide claim 128 , and combinations thereof.130. The lyophilized composition of claim 129 , wherein the disaccharide is selected from the group consisting of sucrose claim 129 , trehalose dihydrate claim 129 , maltose monohydrate claim 129 , and lactose monohydrate.131. The lyophilized composition of claim 128 , wherein the carbohydrate is selected from the group consisting of an alditol claim 128 , hydroxypropyl-cyclodextrin claim 128 , and combinations thereof.132. The lyophilized composition of claim 127 , wherein the lyophilization agent comprises bovine serum albumin (BSA).133. The lyophilized composition of claim 127 , wherein the plurality of fluorescent nanoparticles after lyophilization has a quantum yield that is greater than 80% of a quantum yield of the plurality of fluorescent nanoparticles that have not been lyophilized.134. The lyophilized composition of claim 127 , wherein the plurality of fluorescent nanoparticles after lyophilization comprises a similar or increased quantum yield when dispersed in an aqueous solution as compared to the ...

DIAGNOSTIC METHODS FOR THE DETECTION AND QUANTIFICATION OF BLOOD-RELATED DISEASES

A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field. 1. A diagnostic test kit for carrying out a diagnostic method for the detection of a biomarker in a biological sample , comprising:a first container comprising a plurality of atom transfer radical polymerization (ATRP) initiators;a second container comprising a plurality of radically ATRP polymerizable monomers that are able to form a polymer exhibiting a lower critical solution temperature (LCST) at or above which the polymer precipitates, and a reducing agent; anda device for determining a concentration of a biomarker in a biological sample.2. The test kit according to claim 1 , further including a light source claim 1 , a detector claim 1 , and a temperature control device.3. The test kit according to claim 2 , wherein the device for determining the concentration of the biomarker is a chart or a processor; and wherein the initiators claim 2 , the monomers and the reducing agent are stable for at least two months at 50° C.4. The test kit according to claim 1 , wherein the second container is a cuvette claim 1 , glass capillary claim 1 , microwell plate claim 1 , micro reactor or a test strip.5. The test kit according to claim 1 , wherein the plurality of atom transfer radically polymerizable monomers are N-isopropyl acrylamide monomers claim 1 , wherein the polymerization reaction polymerizes the N-isopropyl acrylamide monomers to form the polymer poly(N-isopropyl acrylamide).6. The test kit ...

ASSAY FOR ANTI-POLYVINYL ALCOHOL ANTIBODIES

An assay kit for use in detecting an anti-polyvinyl alcohol antibody (anti-PVAL), comprises: polyvinyl alcohol (PVAL) attached to a support material; and an indicator reagent comprising a binding member that is specific for a human antibody; wherein the binding member is conjugated to a detectable label, and the binding member is capable of forming a complex with an anti-PVAL antibody. The kit can be used in a method for detecting antipolyvinyl alcohol (anti-PVAL) antibodies in test samples, such as serum, which comprises combining a test sample (e.g., serum) with the PVAL-bound support material to form a binary complex of the PVAL with an anti-PVAL antibody in the sample, followed by reacting the indicator reagent with the binary complex to form a labeled ternary complex, and then detecting the presence or absence of the ternary complex. 1. A kit for use in detecting an anti-polyvinyl alcohol antibody (anti-PVAL) , comprising:(a) polyvinyl alcohol (PVAL) attached to a support material; and(b) an indicator reagent comprising a binding member that is specific for a human antibody;wherein the binding member of the indicator reagent is conjugated to a detectable label, and the binding member is capable of forming a complex with an anti-PVAL antibody.2. The kit of claim 1 , further comprising a wash composition for separating non-complexed materials from the support material when contacted therewith.3. The kit of claim 2 , wherein the wash composition comprises a buffer solution.4. The kit of claim 1 , wherein the detectable label comprises one or more material selected from the group consisting of a protein claim 1 , an enzyme claim 1 , a radioisotope claim 1 , a nucleic acid segment claim 1 , a fluorochrome claim 1 , and a fluorescent protein.5. The kit of claim 1 , wherein the detectable label is an enzyme6. The kit of claim 5 , wherein the indicator reagent affords a colorimetric or chemiluminescent signal in the presence of the enzyme.7. The kit of claim 5 , ...

Flow device

The present invention relates to methods for the detection of an analyte, such as coronavirus (e.g. SARS-CoV-2) or other virus particles and proteins, in a test sample. The invention also provides a flow device for use in such methods. Additionally, there is provided a coronavirus-binding reagent having the structure [sialic acid]-[linker]-[polymer]-[gold nanoparticle] for use in the devices and methods of the invention.

PEPTIDE COMPOSITION AND USES THEREOF

Subject of the invention is a composition comprising at least one fragment of the peptide ESAT-6 and at least one fragment of the peptide CFP-10. Preferably, the fragments comprise at least two sets of peptides, a first set comprising at least one peptide of from about 7 to 14 amino acid residues in length and a second set comprising at least one peptide of from 16 amino acid residues or greater. The invention also relates to diagnostic methods using the composition. 1. A composition comprising at least one fragment of ESAT-6 and at least one fragment of CFP-10.2. The composition of claim 1 , wherein the at least one fragments of ESAT-6 and the at least one fragment of CFP-10 comprise at least two sets of peptides claim 1 , a first set comprising at least one peptide of from about 7 to 14 amino acid residues in length and a second set comprising at least one peptide of from 16 amino acid residues or greater.3. The composition of claim 2 , wherein the at least one peptide of from about 7 to 14 amino acid residues is recognized by CD8+ lymphocytes and the at least one peptide of from 16 amino acid or greater peptides is recognized by CD4+ lymphocytes.4. The composition of claim 1 , further comprising at least one sugar.5. The composition according to claim 4 , wherein the at least one sugar is a non-reducing sugar claim 4 , or wherein the at least one sugar is trehalose.6. A process for measuring cell-mediated immune response activity in a subject claim 4 , said method comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'contacting immune cells comprising lymphocytes obtained from the subject with the composition of ; and'}measuring presence or elevation in a level of an immune effector molecule produced by said immune cells,wherein the presence or level of the immune effector molecule is indicative of a level of cell-mediated responsiveness of the subject.7. The process of claim 6 , wherein either or both of (i) the immune cells are present in a sample of ...

PEPTOIDS THAT BIND SPECIFIC ANTIGENS

Combinatorial libraries were generated providing a vast number of diverse peptoid ligands. From these libraries, ligands were identified which specifically bind molecules associated with autoimmune diseases, such as antibodies specific to aquaporin-4 (AQP4), binding of which to AQP4 causes the autoimmune disease, Neuoromyelitis Optica. Methods of generating peptoid libraries and for diagnosing Neuromyelitis Optica are also provided. 1. A method of identifying ligands which specifically bind to receptors associated with autoimmune diseases , the method comprising:providing a compound library of candidate ligands wherein each ligand is coupled to a support;contacting the library with a control sample and removing ligands associated with non-specific binding and/or specific ligands against antibodies common in healthy people;contacting the remaining ligands with a test sample and a labeled secondary antibody; and, identifying ligands which specifically bind to receptors associated with autoimmune diseases.3. The method of claim 2 , wherein the peptoid of formula (I) is attached to a bead via a linker molecule wherein the linker molecule is non-variable.4. The method of claim 1 , wherein the receptors comprise antibodies claim 1 , T cell receptors or molecules associated with an autoimmune response.5. The method of claim 4 , wherein the receptor is an antibody.6. The method of claim 5 , wherein the antibody is specific for aquaporin 4 (AQP4).7. The method of claim 1 , wherein the substrate comprises: bead claim 1 , a chip claim 1 , a filter claim 1 , a dipstick claim 1 , a membrane claim 1 , a polymer matrix or a well.11. A peptoid combinatorial library produced by the method according to .12. A method of diagnosing Neuromyelitis optica (NMO) comprising:contacting a patient sample with a combinatorial library of peptoids, wherein peptoids which specifically bind to antibodies specific for aquaporin 4 (AQP4) or NMO antigens, are detected.13. The method of claim 12 , ...

Molecular design to suppress desorption of self-assembled monolayers

The compositions described herein include a substrate, wherein the substrate is a metal, metal oxide, metal nitride or a silicon containing material; a self-assembled monolayer (SAM) bonded to the substrate, wherein the self-assembled monolayer comprises: a surface binding unit bonded to the substrate, wherein the surface binding unit is selected from the group consisting of hydroxamates, phosphonates, catechols, halosilanes, alkoxysilanes, phosphonic acids, alkenes, alkynes, alcohols, 1,2-diols, and thiols; a separator unit bonded to the surface binding unit; a mass altering unit bonded to the separator unit; and a detector unit bonded to the separator unit.

Production of polymeric microarrays

A method of preparing a microarray of polymer elements. The method includes providing a substrate surface, providing a plurality of individual monomers in a liquid phase, depositing said monomers as a plurality of discrete monomer elements on the substrate surface, and exposing the plurality of discrete monomer elements to initiating conditions to form polymer elements. A portion of the discrete monomer elements may include more than one type of monomer.

Method for functionalizing a porous membrane covering of an optical sensor to facilitate coupling of an antithrombogenic agent

Methods of covalently attaching heparin to a membrane comprising plasma treating the membrane to produce an amino-functionalized membrane; and reacting the amino-functionalized membrane with heparin under conditions in which heparin becomes covalently attached to the amino-functionalized membrane, wherein said heparin is indirectly attached via a spacer to said amino-functionalized membrane and/or said heparin is attached from a single site in said heparin to a single site on said amino-functionalized membrane or to said spacer. Also disclosed are analyte sensors.

Method for functionalizing a porous membrane covering of an optical sensor to facilitate coupling of an antithrombogenic agent

Methods of covalently attaching heparin to a membrane comprising plasma treating the membrane to produce an amino-functionalized membrane; and reacting the amino-functionalized membrane with heparin under conditions in which heparin becomes covalently attached to the amino-functionalized membrane, wherein said heparin is indirectly attached via a spacer to said amino-functionalized membrane and/or said heparin is attached from a single site in said heparin to a single site on said amino- functionalized membrane or to said spacer. Also disclosed are analyte sensors.

Immobilized substrate, separation gels and method for detecting enzymatic activity

The invention relates to a method for fixing substrate molecules in gel matrices. Said method is characterized in that a substrate is covalently linked to a monomeric or oligomeric structural unit of a gel and the resulting product is polymerized. The invention also relates to gels produced using said method.

Polymerizable compounds and methods for preparing synthetic polymers that integrally contain polypeptides

A method is disclosed for the de novo synthesis of polypeptide-containing polymers. This disclosure also includes a description of, and a method for the preparation of, a class of polymerizable compounds used in the synthesis of polypeptide-containing polymers. These polymerizable compounds are chemical conjugates prepared by covalent linkage of polymerizable organic monomers with specific polypeptides. Soluble monomer/polypeptide conjugates can be polymerized in solution with additional nonderivatized organic monomers to form desired polypeptide-containing polymers. The amount and composition of monomer and monomer/polypeptide conjugates can be varied in order to provide control of (a) molecular spacing, steric accessibility, and number of polypeptide molecules that are integrally incorporated into the polymer backbone, and (b) the chemical and physical structure of the polymer itself. This enables the specific tailoring of polypeptide-containing polymers for particular end-use applications.

Immobilized substrate, separation gels and method for detecting enzymatic activity

The present invention refers to a method for attaching substrate molecules to gel matrices, characterized in that a substrate is covalently bound to a monomeric or oligomeric constitutional unit of a gel and the resultant product is polymerized. The present invention further refers to gels obtainable by this process.

POLYMERIZABLE COMPOUNDS CONTAINING INTEGRAL POLYPEPTIDES AND THEIR APPLICATIONS IN IMMUNE TESTS WITH SEPARATION INDUCED BY POLYMERIZATION.

Production of polymeric microarrays

A method of preparing a microarray of polymer elements (6). The method includes providing a substrate surface (4), providing a plurality of individual monomers in a liquid phase, depositing said monomers as a plurality of discrete monomer elements on the substrate surface (4), and exposing the plurality of discrete monomer elements to initiating conditions to form polymer elements (6). A portion of the discrete monomer elements may include more than one type of monomer.

Peptide composition and uses thereof

본 발명의 대상은 펩티드 ESAT-6의 적어도 1개의 단편 및 펩티드 CFP-10의 적어도 1개의 단편을 포함하는 조성물이다. 바람직하게는, 단편은 펩티드의 적어도 2개 세트를 포함하며, 제1 세트는 약 7 내지 14개의 아미노산 잔기 길이의 적어도 1개의 펩티드를 포함하고, 제2 세트는 16개 이상의 아미노산 잔기의 적어도 1개의 펩티드를 포함한다. 본 발명은 또한 조성물을 사용하는 진단 방법에 관한 것이다.

POLYMERIZABLE COMPOUNDS CONTAINING POLYPEPTIDES AS AN INTEGRAL PART AND THEIR USE IN IMMUNOLOGICAL TESTS OF POLYMERIZATION-INDUCED SEPARATION

Production of polymeric microarrays

A method of preparing a microarray of polymer elements (6). The method includes providing a substrate surface (4), providing a plurality of individual monomers in a liquid phase, depositing said monomers as a plurality of discrete monomer elements on the substrate surface (4), and exposing the plurality of discrete monomer elements to initiating conditions to form polymer elements (6). A portion of the discrete monomer elements may include more than one type of monomer.

Methods for altering surface characteristics of microspheres

Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.

Polymer dot compositions and related methods

Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.

Peptide combinations and application thereof

本发明主题是含肽ESAT‑6的至少一个片段和肽CFP‑10的至少一个片段的组合物。优选地，片段包含至少两组肽，第一组包含长度为约7至14个氨基酸残基的至少一个肽，第二组包含16个或更多氨基酸残基的至少一个肽。本发明还涉及用所述组合物的诊断方法。

New covalently crosslinked imprint poly:peptide, e.g. enzyme

The invention relates to imprint polypeptides covalently cross-linked and having a fixed and stabilised arrangement, process for the preparation and uses thereof. The properties of said imprint polypeptides (VIP) covalently cross-linked are present in aqueous and in organic solvent systems, and can therefore be used in both types of system.

Method for recovering extracellular vesicles

本发明提供能够高效地回收细胞外囊泡的、代替以往技术的方法。更具体而言，本发明提供包含以下(a)和(b)的细胞外囊泡的回收方法：(a)将(i)含细胞外囊泡的试样、(ii)固定有与细胞外囊泡膜具有亲和性的物质的粒子和(iii)聚合物进行混合，得到包含(i’)借助所述物质与细胞外囊泡进行了结合的目标粒子和(ii’)聚合物的混合液；和(b)从所述混合液中分离所述目标粒子。在所述工序(a)和(b)之间，优选进一步包含：使所述混合液的粘度降低的步骤。

Fixing substrate molecules in gel matrixes

A process for fixing substrate molecules in gel matrixes, comprises covalently binding the substrate molecule to a monomer or oligomer building block of the gel and polymerising the resulting compounds. Independent claims are also included for: (1) an immobilised substrate produced by the method above; and (2) gel monomers or oligomers with covalently bound substrate molecules.

Production of polymeric microarrays

Methods for altering surface characteristics of microspheres

Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.

Three-dimensional hydrogel-graphene-based biosensor and preparation method thereof

The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Detection and Quantification of Molecular Species

The invention relates to a conjugate comprising a binding element and a self-polymerising biopolymer. The invention also relates to a kit comprising the conjugate and self-polymerising biopolymer subunits. The invention also relates to a method of using the conjugate to measure the concentration of a target substance comprising binding the conjugate to the substance, isolating the bound conjugate, polymerising biopolymer filament, and calculating the concentration of the target substance.

펩티드 조성물 및 그의 용도

본 발명의 대상은 펩티드 ESAT-6의 적어도 1개의 단편 및 펩티드 CFP-10의 적어도 1개의 단편을 포함하는 조성물이다. 바람직하게는, 단편은 펩티드의 적어도 2개 세트를 포함하며, 제1 세트는 약 7 내지 14개의 아미노산 잔기 길이의 적어도 1개의 펩티드를 포함하고, 제2 세트는 16개 이상의 아미노산 잔기의 적어도 1개의 펩티드를 포함한다. 본 발명은 또한 조성물을 사용하는 진단 방법에 관한 것이다.

Three-dimensional hydrogel-graphene-based biosensor and preparation method thereof

The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Detection assay

The present invention relates to improved detection assays via the utilisation of a substrate with an analyte immobilised on its surface by means of a linking agent and a cross-linker. The present invention also relates to a method of producing a substrate for use in detection assays and a method of detecting an analyte using said substrate.

Verfahren zur veränderung der oberflächeneigenschaften von mikrokügelchen

Soluble and polymeric metal complexing materials for measurement of sugars and related molecules in solution

Methods of determining relative potency of glatiramer acetate and use thereof in preparing a batch of glatiramer acetate as acceptable for pharmaceutical use

Methods of determining relative potency of glatiramer acetate and use thereof in preparing a batch of glatiramer acetate as acceptable for pharmaceutical use

A method of determining relative potency of glatiramer acetate (GA) is provided. Accordingly there is provided a method comprising: immunizing a mammal with Poly- YAK copolymer (pYAK); preparing a primary culture of T-cells from the immunized mammal; stimulating samples of the primary culture of T-cells with a reference standard batch of GA or a batch of GA; determining a stimulation parameter of the cells in each sample following a predetermined incubation time; and comparing the stimulation parameter in the cells so as to determine the relative potency of the batch of GA. Also provided are methods of preparing a batch of GA as acceptable for pharmaceutical use and treating multiple sclerosis (MS) in a subject.

Sensors for sugars and other metal binding analytes

Method for harvesting extracellular vesicles

Early entry - no title

Three-dimensional hydrogel-graphene-based biosensor and preparation method therefor

A three-dimensional hydrogel-graphene-based biosensor and a preparation method therefor, relating to the technical field of biosensors. The three-dimensional hydrogel-graphene-based biosensor comprises a substrate, an electrode layer, a graphene thin film and a three-dimensional hydrogel material layer which are sequentially stacked; the three-dimensional hydrogel material layer is formed by a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerizing a raw material comprising an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule in which an acrylamide group is modified. The three-dimensional hydrogel-graphene-based biosensor has good stability and high sensitivity.

Diagnostic methods for the detection and quantification of blood-related diseases

A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field.

Detection assay

The present invention relates to improved detection assays via the utilisation of a substrate with an analyte immobilised on its surface by means of a linking agent and a cross-linker. The present invention also relates to a method of producing a substrate for use in detection assays and a method of detecting an analyte using said substrate.

细胞外囊泡的回收方法

本发明提供能够高效地回收细胞外囊泡的、代替以往技术的方法。更具体而言，本发明提供包含以下(a)和(b)的细胞外囊泡的回收方法：(a)将(i)含细胞外囊泡的试样、(ii)固定有与细胞外囊泡膜具有亲和性的物质的粒子和(iii)聚合物进行混合，得到包含(i’)借助所述物质与细胞外囊泡进行了结合的目标粒子和(ii’)聚合物的混合液；和(b)从所述混合液中分离所述目标粒子。在所述工序(a)和(b)之间，优选进一步包含：使所述混合液的粘度降低的步骤。

聚合物点组合物和相关方法

提供了冻干的发色聚合物点组合物。还公开了制备和使用所述冻干组合物的方法，在水溶液中分散所述冻干组合物的方法以及提供所述组合物的试剂盒。

Diagnostic methods for the detection and quantification of blood-related diseases

A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field.