Микрохроматограф с бинарными колонками на плоскости

Изобретение относится к газовой хроматографии и может быть использовано для эффективного экспресс-анализа сложных смесей веществ природного и техногенного происхождения. Микрохроматограф содержит сменные независимо управляемые аналитические модули для анализа компонентов сложных смесей, каждый из которых содержит термостатируемую микрохроматографическую колонку, выполненную на плоских пластинах с каналами для сорбента, изготовленными методом микрофрезерования, термостатируемый планарный микродозатор исследуемой пробы и термостатируемую детектирующую систему, отличающийся тем, что термостатируемая микрохроматографическая колонка является составной и включает в себя две микрохроматографические колонки на плоскости, при этом аналитический модуль дополнительно снабжен, по крайней мере, одним малогабаритным электропневмораспределителем для управления составными колонками. Техническим результатом является повышение чувствительности, увеличении разделительной способности и уменьшении времени анализа ...

СИСТЕМА И СПОСОБ АВТОМАТИЗАЦИИ НАБИВКИ КОЛОНКИ СРЕДОЙ

... 1. Способ автоматизации набивки колонки, включающий: !определение суспензионной концентрации хроматографической среды; ! определение объема колонки на по меньшей мере одной хроматографической колонке; ! определение коэффициента сжатия хроматографической среды на по меньшей мере одной хроматографической колонке; ! расчет объема суспензии на основе определенного объема колонки, определенного коэффициента сжатия и определенной суспензионной концентрации; ! размещение расчетного объема суспензии в суспензионном баке; ! установка по меньшей мере одной хроматографической колонки для подачи расчетного объема суспензии и набивки по меньшей мере одной хроматографической колонки; ! отслеживание подачи расчетного объема суспензии в по меньшей мере одну хроматографическую колонку; ! автоматическое прекращение подачи расчетного объема суспензии в по меньшей мере одну хроматографическую колонку, когда расчетный объем суспензии равен определенному объему колонки. ! 2. Способ по п.1, в котором отслеживание ...

Impregnation of capillary column - in which charge of impregnating soln. is forced through capillary column at constant speed

Capillary tubes made from long sections after the dynamic method for the gas chromatographic sepn. of mixtures of materials, have a thin film of sepn. fluid applied to the inner capillary surface. The novelty is that a charge of the impregnating soln. is forced through the capillary column at a constant speed, at the entrance of which a buffer vessel with defined vol. is connected which is pressurised to a given value; that the initial vol. of the buffer vessel is given by equation (I), that the initial pressure in the buffer vessel is given by equation (II) where: d is the capillary internal dia. (mm); df is the film thickness in the stationary phase (mm).

Chromatographiesäule mit verbesserten Aufschlämmeigenschaften

Verfahren und Vorrichtung zum Befuellen von Chromatographiesaeulen

Fuellmaterial fuer chromatographische Saeulen

Vorrichtung zum Packen von Chromatographiesäulen

Chromatography column and maintenance method

Method and apparatus for forming an homogeneous mixture of chromatography media in a vessel

APPARATUS AND METHOD FOR CHAGING AND DISCHARGING OF CHROMATOGRAPHY COLUMN BED

IMPROVED CHROMATOGRAPHY COLUMN.

A chromatography column piston 14 is adjustable in height within the column tube 4 by means of a friction clamp 22 which furthermore includes a threaded engagement with the associated end cap 8 of the column. As a result, with the friction clamp released the piston rod 16 can be rapidly traversed to or near a desired position, and the clamp then engaged following which cooperation of said threads allows fine tuning of the level of the piston 14 by fine adjustment of the position of the friction clamp relative to the end cap 8.

Packing chromatography columns

A method and system for packing an axial flow chromatography column 101 with a bed 109 of particulate medium of a target bed height wherein the column comprises a housing having an elongate tubular side wall 111 and opposed axially spaced first 112 and second end units 113 separated by the side wall. At least one of the end units is axially moveable by drive means (not shown) relative to the other end unit and further comprises a first filter 104 adjacent the first end unit and a second filter 106 adjacent the second unit which together with the side wall define an enclosed space for containing a bed of particulate medium. The relative movement of the first and/or second filter alters the bed height. The first end unit comprises a first valve for filling the bed space with the particulate medium 121 and a first port 133 for adding a liquid to or removing a liquid from the bed space and the second end unit comprises a second port 140 for adding liquid to or removing liquid from the bed space ...

Chromatographic columns

... 1,086,592. Chromatography column closure device. CESKOSLOVENSKA AKADEMIE VED. March 26, 1965 [April 13, 1964], No. 12891/65. Heading B1X. A device for compensating volumetric changes in chromatography column fillings comprises bracket 3 secured to fixed member 1 and having guide means to locate the bracket about closure 5 of the chromatography column 2, a clamp 4 attached to the closure below the guide means, lever means 14 at one end suspended from the bracket by pull rods 16 and at the other end attached to connecting rods 18 which engage with pins 17 on clamp 6, and spring 8 which bears on lever 14 and hence causes the urging of closure 5 into the column. In an alternative embodiment (Fig. 3, not shown), a U-shaped piece of resilient sheet metal (22) is used instead of the lever 14, and the action of spring (8) may be suppressed when desired by means of catches (28) suspended from toggle lever (30) operated by pusher member (32). In either form of apparatus the column filling may be ...

CHROMATOGRAPHY COLUMN AND END PLUG ASSEMBLY

Chromatography columns,systems and methods

Chromatography column

MICROPARTICLE COLUMN PACKING

INTELLIGENT SYSTEM AND METHOD TO AUTOMATED LUGGAGES OF CHROMATOGRAPHY COLUMNS

PROCEDURE FOR PACKING CAPILLARY AULA WITH PARTIKELFÍRMIGEN MATERIALS

RECTIFYING COLUMN FOR THE LIQUID CHROMATOGRAPHY.

VORRICHTUNG ZUR KONTINUIERLICHEN ANNULAREN CHROMATOGRAPHIE

CHROMATOGRAFIEGERAET AND PROCEDURES FOR ITS ENTERPRISE.

ENTRANCE VALVE, ITS USE IN A SEPARATING EQUIPMENT AND EQUIVALENT PROCEDURE

CHROMATOGRAPHI DEVICE

CHROMATOGRAPHY COLUMN WITH IMPROVED MIXING INTO A PASTE WITH CHARACTERISTICS

PROCEDURE FOR PACKING CHROMATOGRAPHY COLUMNS

Conditioning of packed chromatography columns

The present invention relates to a method of conditioning a dry-packed chromatography column, which method comprises providing a column packed with inert and hydrophilic chromatography resin; and driving a solvent or solvent mix across the column. The solvent(s) are selected to be less hydrophilic than the chromatography resin; and the rate of the solvent flow is controlled to a rate which does not cause the system pressure to exceed a predefined threshold. The present invention is advantageously used in FLASH chromatography, where the generation of heat may pose problems e.g. to plastic frits or filters at high flow rates and/or wetting of dry resins.

Ion chromatography system for exchanging an ion exchanger in a suppressor and a suppressor means

SEPARATION COLUMN FOR LIQUID CHROMATOGRAPHY AND SEALING ARRANGEMENT THEREFOR

CHROMATOGRAPHY COLUMN AND MAINTENANCE METHOD

A method of maintenance of a chromatography column is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. The provision of guide elements which can be reversibly attached to the column facilitates removal or insertion of column components.

ADSORPTION COLUMNS FOR USE IN RADIOIMMUNOASSAYS

Absorption columns are provided which can be utilized in radioimmunoassay systems, such as those involving the separation of antibody-antigen complexes from free antigens. The preparation of the columns includes the treatment of retaining substrate material to render it hydrophilic, preparation and degassing of the separation material and loading the column.

PROCESS FOR LARGE SCALE CHROMATOGRAPHY

MOLECULAR SEPARATION COLUMN AND USE THEREOF

CHROMATOGRAPHY SYSTEM WITH TILT-PREVENTION STRUCTURE AND ASSOCIATED PROCESS

Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube (1) has a process fluid input device (41) at the bottom and a movable piston (2) in the top. The piston (2) is enclosed in the column by a top cover plate (3). The piston body (21) has a peripheral inflatable seal (23), and is connected by an open frame (85) to a contact ring (81) above which carries another inflatable annular member (83) to contact the tube wall. Process fluid leaves the operating volume (13) through a central opening of the piston and flexible hose (9), through the enclosed space and out through the cover plate (3). The space (77) above the piston (2) can be pressurised to control piston movement. The contact ring (81) maintains piston alignment. The inflatable seals (23,83) can be inflated or deflated to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound ...

CHROMATOGRAPHY COLUMN SUPPORT

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.

CHROMATOGRAPHY COLUMNS WITH CONTINUOUS BEDS FORMED IN SITU FROM AQUEOUS SOLUTIONS

A chromatography column with a continuous solid bed spanning the cross section o f the column, the bed containing channels large enough for hydrodynamic flow, is p repared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solutio n. The monomers include a monofunctional monomer such as a vinyl, allyl, acrylic, o r methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), the to tal monomer concentration being in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammonium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impa rt specialized separation capabilities, notably anion and cation exchange, can be i ncluded in the monomer mixture.

ACCESS VALVE DEVICES, THEIR USE IN SEPARATION APPARATUS, AND CORRESPONDING METHODS

Füllmaterial für gaschromatographische Trennsäulen

Trennsäule für Geräte zur Gaschromatographie

Vorrichtung zur Trennung von Substanzgemischen mittels Gaschromatographie

Procédé pour la séparation chromatographique d'un fluide, appareil pour la mise en oeuvre de ce procédé et application de ce procédé

Verfahren zur Herstellung einer Trennsäule für die Chromatographie

Gerät zur chromatographischen oder elektrophoretischen Trennung gelöster Stoffgemische zu analytischen, präparativen oder diagnostischen Zwecken

Füllkörper, der insbesondere für Chromatographiekolonnen geeignet ist

Verfahren zur Herstellung von Trennsäulen für Gaschromatographie

Verfahren zur Herstellung von Kapillarsäulen für die Gaschromatographie

Procédé pour la séparation de substances contenues dans un mélange de composés chimiques et colonne chromatographie gaz-solide par la mise en oeuvre du procédé

Chromatographische Trennsäule

Procédé de préparation d'une masse frittée, masse frittée résultant du procédé et utilisation de ladite masse frittée

Verfahren und Vorrichtung zur Anzeige bzw. Messung mindestens eines oxydierbaren bzw. reduzierbaren Bestandteils eines Eluats auf elektrochemischem Weg

Porous synthetic resin structure

Apparatus for division-separation operation has a porous filling with a three dimensional pore structure, which serves to pass at least one phase, the adjoining faces of the structure serving as support for at least one other phase. The porous filling is characterised by a combination of (a) a large number of the connected hollow pores are enclosed by solid parts, which also have a three dimensional cohesive structure; (b) the hollow pores are interrupted in all cross-sections of the filling, in a direction perpendicular to the direction of flow, by solid particles, which are also mutually connected; and (c) the porosity characteristics of the filling as a whole are essentially homogeneous, at least perpendicular to the direction of flow.

Procédé de chromatographie gazeuse et appareil pour sa mise en oeuvre

Verfahren zur Herstellung einer Kapillare für die Gaschromatographie

PROCEDURE FOR PACKING A CHROMATOGRAPHY COLUMN.

PROCEDURE FOR THE CHROMATOGRAPHIEREN.

METHOD OF LOADING CHAMBER FOR LAYER OF PARTICLES IN ANNULAR CHROMATOGRAPH

METHOD OF DETERMINING CONTENT OF SUSPENSION CHROMATOGRAPHIC RESIN

CHROMATOGRAPHY DEVICE

METHOD OF PREPARATION OF COLUMNS Of ADSORPTION FOR RADIOIMMUNOLOGICAL ANALYSES

Adsorbent columns furnished with diamantiferous matter and their use in the separation of gases, especially with gas containing of fluorine or of composed of fluorine

Manufactoring process of separating columns of gas chromatography

PROCEDE ET DISPOSITIF DE REMPLISSAGE DE COLONNES CHROMATOGRAPHIQUES

PROCEDE ET DISPOSITIF DE REMPLISSAGE D'UNE COLONNE CHROMATOGRAPHIQUE AVEC UN GARNISSAGE CONTENU DANS UNE SUSPENSION FLUIDE. DES MOYENS DE MISE EN VIBRATION 6 SONT DISPOSES A LA PERIPHERIE DE LA COLONNE 4 ET DE LA CHAMBRE DE COMPRESSION 1 QUI LUI EST RAPPORTEE ET LE PISTON 2 EST TRAVERSE PAR DES CANALISATIONS D'ENTREE OU DE SORTIE D'UN FLUIDE DE CHROMATOGRAPHIE TEL QU'UN GARNISSAGE 1A OU SUSPENSION DANS UN LIQUIDE INTRODUIT DANS LA CHAMBRE DE COMPRESSION 1. APPLICATION A L'OBTENTION D'UNE COLONNE DE CHROMATOGRAPHIE A GARNISSAGE COMPACT.

METHOD OF CHROMATOGRAPHY

Sätt och anordning för vätskekromatografi

EXPANDED BED CHROMATOGRAPHIC SEPARATION COLUMN FOR BIOCHEMICAL SEPARATION PROCESS AND TECHNICAL PROCESS THEREOF

Provided are an expanded bed chromatographic separation column for a biochemical separation process and a technical process thereof. By providing a pressure equalization liquid distributor having a self-cleaning function below a porous blocking sieve plate (301) at the bottom of the expanded bed, and providing an upper part nozzle assembly having a backflush cleaning function at the top of the expanded bed, the expanded bed chromatographic separation column is suitable for treating a feedstock liquor (307) having solid particulate impurities, and at the same time a better distribution of the feedstock liquor (307) added into the expanded bed can be achieved, ensuring that the fluid passed through the expanded bed layer displays a state of piston flow. The expanded bed chromatographic separation column and the technical process thereof have the following advantages: they greatly increase the separation theoretical value and the chromatographic separation efficiency of the expanded bed; improve ...

AUTOMATED PACKING SYSTEM AND METHOD FOR CHROMATOGRAPHY COLUMNS

A column packing system comprises a control unit provided with software for monitoring the column interior pressure and/or flow through the column for use in calculating the breakpoint where a movable adapter comes into contact with a consolidated bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

DELIVERY AND ASSESSMENT SYSTEM FOR THE AUTOMATED MANUFACTURING OF HIGH PERFORMANCE NANOFLUIDIC SEPARATION DEVICES

A multi-column packing system and packed bed assessment system for producing small bore LC columns of superior quality, improved column-to-column reproducibility, and lower cost than current commercially available columns. The process utilizes an automated multi-column packing system with an integrated sensor that detects when the slurry-packed bed has reached the desired length. The optical packed bed assessment system examines the packed bed within the column and detects any packing inconsistencies or voids within the column. The multi-column packing system makes automated connections to tubes via an automated compression mechanism.

A CAKY CHROMATOGRAPHIC COLUMN AND THE METHOD FOR PRODUCING IT AND ITS APPLICATIONS

The present invention discloses a caky chromatographic column and the method for producing it and its application. The caky chromatographic column includes a chromatographic medium column with an inlet and an exit for mobile phase and chromatographic medium packed in the inner cavity of the chromatographic medium column. The rate of thickness to radial length of the said inner cavity of the chromatographic medium is less than or equal to 1. The method for producing the caky chromatographic column comprises the steps of: 1) producing a chromatographic medium column that includes upper and lower pressing plates combined together with the inlet and exit for the mobile phase and a column body with at least one side-hole, the rate of the thickness to the radial length of the inner cavity of the chromatographic medium is less than or equal to 1; 2) injecting the chromatographic medium into the inner cavity of the chromatographic medium column through the side-hole of the chromatographic column ...

High efficiency sol-gel gas chromatography column

A capillary column (10) includes a tube structure having inner walls (14) and a sol-gel substrate (16) coated on a portion of inner walls (14) to form a stationary phase coating (18) on inner walls (14). The sol solution used to prepare the sol-gel substrate (16) has at least one baseline stabilizing reagent and at least one surface deactivation reagent resulting in the sol-gel substrate (16) having at least one baseling stabilizing reagent residual and at least one surface deactivating reagent residual. A method of making the sol-gel solution is by mixing suitable sol-gel prescursors to form the solution, stabilizing the solution by adding at least one baseline stabilization reagent, deactivating the solution by adding at least one surface deactivation reagent to the solution, and reacting the solution in the presence of at least one catalyst.

Multicapillary column for chromatography and sample preparation

A multicapillary column especially useful for liquid chromatography and sample preparation comprising a plurality of uniform capillaries coated with an insoluble stationary phase, wherein the thickness of the stationary phase is correlated with the radius of the individual capillaries for high efficiency.

Open Tubular Capillaries Having a Connecting Layer

The invention relates to a method for producing open tubular capillaries containing monolithic sorbents and to said capillaries. The inventive capillaries are characterised by a connection layer between the capillary wall and the sorbent, said layer ensuring a particularly good adhesion of the sorbent to the capillary wall.

Method for forming a gel bed in a column for liquid chromatography and an axially adjustable-type column device used for this method

To form a gel bed in a cylindrical column for liquid chromatography, a gel bed section is provided between upper and lower liquid-tight partition members, positioned apart from each other at upper and lower positions in the column. Liquid is continuously percolated downward through the gel bed section to form a gel bed in the gel bed section and a liquid pressure is applied onto the upper partition to move the partition member downward following a downward movement of the upper surface of the gel bed.

METHOD OF PREPARING CHROMATOGRAPHIC COLUMNS

METHOD AND APPARATUS FOR CHROMATOGRAPHIC SEPARATIONS WITH SUPERFICIALLY POROUS GLASS BEADS HAVING SORPTIVELY ACTIVE CRUSTS

Separation column containing porous matrix and method of packing column

A mixture of chromatographic particles and a solution of water, alcohol and metal alkoxide may be injected by means of a syringe into a capillary column as a gel. The volatile components in the gel are evaporated by means of heating and gas pressure reduction to form a porous sol-gel glass matrix attached to the inner wall of the separation channel. The pores are large enough for the passage of protons, neutral and ionic species but are too small to permit significant leaching of the chromatographic particles. The separation column so formed requires no frits to maintain the glass matrix in place in the column. Electrical potential difference and/or pressure difference may be applied to cause fluid flow in the separation column to cause electrophoretic and chromatographic separation.

Method and device for extracting filter frits from liquid chromatographic columns

A method and apparatus for removing a small metal filter from a liquid chromatographic column, wherein a retaining member is clamped around the tube. A first centering device is releasably centered on the retaining member in alignment with the filter, whereupon a drill is slidably guided through the centering device for forming a hole of selected depth in the filter. The first centering device is removed and a second centering device is supported on the retainer, which second centering device has a threaded opening therethrough aligned with the filter. An extractor is threaded into the opening so that the end of the extractor is disposed directly adjacent the filter. A tapping unit having an elongated thread-forming tap is slidably inserted through an opening formed in the extractor, which opening is aligned with the hole formed in the filter. The tapping unit is threaded inwardly so that the tap threadably engages the filter and until a handle on the tapping unit abuts the extractor. The ...

Suppression of electroosmosis with hydrolytically stable coatings

Surfaces of silica-containing materials, such as the inner walls of silica capillaries, used in chromatographic, particularly electrophoretic, separations are coated with an organic polymer layer to reduce or eliminate surface charges. The layer is applied by first converting the silanol groups on the surface to silicon halide groups, then reacting these groups with an organometallic reagent having a terminal ethenyl moiety, preferably vinyl or allyl lithium or a vinyl or allyl magnesium halide, to convert the silicon halide groups to Si--R groups where the R retains the terminal ethenyl moiety, and finally reacting these ethenyl groups newly attached to the surface with a neutral organic monomer in an addition polymerization reaction to form a monomolecular noncrosslinked polymer layer over the surface. The resulting polymer layer is linked to the silica directly through a Si--C bond which is stable over a wide range of pH conditions.

CHROMATOGRAPHY SYSTEM

Polymer packing material for liquid chromatography and a producing method thereof

A method of producing a polymer packing material for liquid chromatography includes the steps of swelling seed polymer particles to form swelled particles, polymerizing monomers in the swelled particles under existence of diluent, and removing the diluent to form porous polymer particles, wherein the step of polymerizing the monomers is conducted under existence of a compound having a conjugated double bond not reactive with the monomers and having a molecular weight of 100-500. Alternatively, the polymerization process may be conducted by an oxidation reduction polymerization process. Further, the porous polymer particles obtained after removal of the diluent may be subjected to a heat treatment process at a temperature of 100 DEG C. or higher.

Method and devices for dry loading of chromatography resins

A module (1) for dry loading and unloading of a chromatography resin, a chromatography column (3) and a method for using such a module (1). The column (3) comprises an inlet valve (39) adapted to load dry chromatography resin particles and an outlet port (35) for pumping the air from de column (3). The outlet port (35) is located above the inlet valve (39).

IMPROVED CHROMATOGRAPHY COLUMN

The present invention relates to a chromatography column that removes the longitudinal load from the column tube (52) altogether and transfers it to a yoke (56) and stanchion (54) arrangement situated around but external to the column tube (52). By using the yoke/stanchion system, the central adjuster (60) can be used to move the top end (66) into and out of the column tube (52). Thus, when internal pressure is applied to the column, the tube (52) experiences no longitudinal load. Additionally, the yoke (56) and stanchion (54) design allows for complete removal of the end (66) from the tube without cumbersome disassembly. Moreover, the yoke (56) provides a place to retain the end (66) while the tube (52) is being filled, emptied, cleaned or repaired.

Intelligent system and method for automated packing of chromatography columns

Chromatography column

カラム及び媒体充填を自動化するためのシステム及び方法

LIQUID CHROMATOGRAPH COLUMN DEVICE

УСТРОЙСТВО И СПОСОБ АВТОМАТИЧЕСКОГО УПЛОТНЕНИЯ ДЛЯ ХРОМАТОГРАФИЧЕСКИХ КОЛОНОК

Изобретение относится к системе уплотнения рабочего материала для колонки. Способ уплотнения для колонок включает обеспечение наличия автоматического блока управления, который содержит программное и аппаратное обеспечение для автоматической работы. Также способ включает обеспечение наличия колонки, содержащей подвижный адаптер. При этом подвижный адаптер выполнен с возможностью перемещения путем нагнетания жидкости в герметичное пространство между адаптером и крышкой колонки или откачивания из него жидкости. Кроме того, способ включает использование блока управления для отслеживания перемещения подвижного адаптера в колонке. При этом блок управления программируют для наполнения колонки рабочим материалом слоя сорбента в соответствии с текущими параметрами рабочего материала. К текущим параметрам относятся заданная высота уплотнительного слоя, объем суспензии с заданной концентрацией частиц, скорость опускания адаптера и требуемая степень сжатия сорбента. Также заявленный способ включает ...

Способ хроматографического разделения полиолов

С1ЮСОБ ХРОМАТОГРАвИЧЕСКОГО : РАЗДБЛЕНШ ПОШОЛОВ в хроматопЕ афической колонке, заполненной частицами ионробменной смолы, при котором через хроматографическую колонку непрерывно пропускают подш1жную ясидкум фазу, периодически вводят р.азделяемую смесь полиолов и на выводе колонки отбирают фракции разделенных компонентов, отличающийся тем, что, с целыо повышения эффективности разделения, в качестве ионообменной смолы используют частиф суль мрированной nojraсгарольной катионообменной смолы с поперечными связями, содержанием 3,52 дивинипбензола и средним размером частиц 0,24) ,5 мм,причем 95% частиц смолы имеют размеры в диапазоне от среднего размера частиц, в качестве подвижной фазы О) используют воду, а раствор разделяемых полиолов вводят в колонку со .скоростыо .0,15-0,6 м/ч.

Adsorption and retrieval of mutually react- - ive components of a gaseous mixture for rev

The individual component gases of the mixture are passed through partial fillings of selectively adsorptive material in a separating column. A ring oven surrounding a part of the column may be used to create a constant temperature gradient in that part of the column. When the gases have been adequately adsorbed, the system may be put on reverse flow and the gas components extracted individually and in turn by moving hte ring oven over that section of the filling into which the individual gas has been adsorbed. An inert gas is passed through the column during this phase.

Chromatographisches Trennverfahren und Vorrichtungen zur Durchfuehrung des Verfahrens

Gradient-Formkoerper,insbesondere zur Anwendung bei der Chromatographie

Siloxane-Immobilized particulate stationary phases for chromatographic separations and extractions

COLUMN PACKING DEVICE

... 1457273 Chromatographic column packing device WELLCOME FOUNDATION Ltd 23 May 1974 [23 May 1973] 24629/73 Heading B1H A chromatographic column packing device comprises a drive plate 1 for receiving column locating means, slidably coupled through a drive shaft 11 to driving means 8 for rotating said drive plate and carrying cam following means 18 cooperating with a climb-face cam 16, and a rotatory member 19 mounted on the drive shaft and having a contact arm 20 positioned for intermittent impact with a cooperating member 21 on a shaft 24 supporting tappers 28 resiliently biassed relative to a column 4. The column is filled from a reservoir 33 via funnel 6, and during filling is simultaneously rotated, vertically reciproated and tapped to obtain even packing.

METHOD FOR PRODUCING COLD-WATER SOLUBLE GELATIN DESSERT MIXES AND PRODUCTS THEREFROM

A novel column cartridge for a chromatography apparatus, particularly HPLC, is comprised of a hollow metal tube, open at both ends and having internal shoulders facing each open end, and a pair of end plugs each having a frusto-conical shape at its outer end. The end plugs are sealed into place by rolling the tube end to compress it against the frustum surface, leaving a short length of plug extending beyond the tube end for compression sealing against a conventional holder or mounting device.

A method of producing a column for partition chromatography

A capillary column suitable for partition chromatography is made by filling a capillary tube with a bubble-free suspension or dispersion containing all the components of the fixed phase, closing one end of the tube and evaporating the liquid dispersant by heating the tube progressively from the open end. The temperature of the tube after evaporation of the liquid dispersant is preferably kept above the dew point of that liquid. The suspension which may be made using a high speed mixer or colloid mill, may comprise one or more active solid sorbents which are preferably finely divided, suspended in one or more high-boiling liquids which form a film on the particles when the coating is dried. The tube may be filled by the application of pressure and the liquid dispersant evaporated using a vacuum. The inside of the tube may be etched or coated with a lacquer or metal, e.g. silver, before the tube is filled with the dispersion. In one embodiment, the liquid dispersant is evaporated simultaneously ...

Chromatography column

Obtained is a column in which, at the time of chromatography, a filler can be supplied in an optimally compressed state, and a test-object-containing mobile phase is prevented from leaking out from a tube. A column 10 is provided mainly with a cylindrical tube 100, a plug 200, a first filter 310, a second filter 320, a stopper 400, and a connector 500. The tube 100 is provided mainly with: a cylindrical storage part 110 with a bottom; and a bottom-side connection part 130 which is cylindrical and which is connected to the bottom of the storage part 110. The storage part 110 and the bottom-side connection part 130 are coaxially provided. The plug 200 is provided mainly with: a coaxially provided cylindrical outer cylindrical part 210; and an inner cylindrical part 220 provided on the inner circumferential side of the outer cylindrical part 210. The first filter 310 and the second filter 320 are such that a filler 330 cannot pass therethrough. The stopper 400 is provided mainly with a stopper ...

Chromatography column and maintenance method

A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device.

Method for conducting maintenance on a chromatography column

The present invention relates to methods for conducting maintenance on chromatography columns used in industrial-scale chromatography. In particular, the invention is concerned with safer methods for performing maintenance on such columns, such as cleaning and replacing bed supports, distributors, nozzles, O-rings and other column components, by the use of a handling device to support, lift, carry and manipulate such column components.

Method and System for Transferring Separation Resin

A method and a system and a container system for transferring separation resin from at least one first container (′) to a second container (′), wherein said first container is a deformable, single-use separation resin storage container, said method comprising the steps of:—preparing (S1) the at least one first container by providing a deformable, single-use container comprising an outlet port (′) with a predefined volume of separation resin in a storage solution;—fluidizing (S3) the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;—fluidically connecting (S5) the outlet port (′) of the at least one first container to an inlet port () of the second container;—transferring (S7) separation resin from the at least one first container to the second container by generating a pressure difference between an interior of the second container and an interior of the first container where the pressure is lower in the second container. 1. A method for transferring separation resin from at least one first container to a second container , wherein said first container is a deformable , single-use separation resin storage container , said method comprising the steps of:preparing the at least one first container by providing a deformable, single-use container comprising an outlet port with a predefined volume of separation resin in a storage solution;fluidizing the separation resin in the at least one first container to provide a resin slurry, said fluidizing being performed by mechanical interaction to the first container from an outside of the first container to provide a deformation of said first container;fluidically connecting the outlet port of the at least one first container to an inlet port of the second container;transferring separation resin from the at least one first ...

GUARD COLUMN AND METHOD FOR PRODUCING GUARD COLUMN

Provided is a guard column including a filling part having a length of 2.0 cm to 3.5 cm formed of a filler, in which the filler is made of porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, and a pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more. 1. A guard column , comprisinga filling part having a length of 2.0 cm to 3.5 cm formed of a filler, whereinthe filler includes porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm, anda pressure difference when an aqueous solution is fed at a linear flow rate of 2.1 cm/min is 4.0 MPa or more.2. The guard column according to claim 1 , wherein the porous silica gel has an average pore size of 25 nm to 35 nm and a specific surface area of 100 m/g to 300 m/g.3. The guard column according to claim 1 , the column is used for size-exclusion chromatography in which a column using the filler containing porous silica gel and a light scattering detector are used.4. A liquid chromatograph comprising the guard column according to claim 1 , a size-exclusion chromatography column filled with a filler containing porous silica gel claim 1 , and a light scattering detector in this order from an upstream side.5. A method of analyzing a polymer compound claim 1 , which comprises:{'claim-ref': {'@idref': 'CLM-00004', 'claim 4'}, 'analyzing a polymer compound with size-exclusion chromatography using the liquid chromatograph according to .'}6. A method for producing a guard column according to claim 1 , comprising:a step of filling a filler including porous silica gel having a hydrophilized surface and an average particle size of 1.5 μm to 2.5 μm at a filling pressure of 12 MPa to 40 MPa per 1 cm in length to form a filling part having a length of 2.0 cm to 3.5 cm.7. The guard column according to claim 1 , wherein a diameter of the filling part is 0.4 cm to 0.9 cm.8. The guard column according to ...

A MICROFLUIDIC DEVICE AND METHODS FOR MANUFACTURING SAME

The present invention relates generally to a sample processing device, such as a microfluidic device, comprising a substrate, wherein the substrate comprises a plurality of channels configured to transport a fluid, and wherein the plurality of channels are substantially coated with lubricin, or a functional variant thereof. Also disclosed herein are methods of manufacturing such devices, methods of preventing fouling of a channel in a device using lubricin, or a functional variant thereof and methods of controlling the electrokinetic flow of an analyte through a channel that is substantially coated with lubricin, or a functional variant thereof. Also disclosed herein is chromatographic material for the electrophoretic and/or chromatographic separation of an analyte, wherein the chromatographic material is substantially coated with lubricin, or a functional variant thereof. 1. A microfluidic device comprising a substrate , wherein the substrate comprises a plurality of channels configured to transport a fluid , and wherein the plurality of channels are substantially coated with lubricin , or a functional variant thereof.2. The device of claim 1 , wherein the plurality of channels comprise a surface selected from the group consisting of a hydrophobic surface claim 1 , an anionic surface claim 1 , a polar surface and combinations thereof.3. The device of claim 2 , wherein the plurality of channels comprise a hydrophobic surface.4. The device of claim 3 , wherein the hydrophobic surface is a gold surface.5. The device of claim 3 , wherein the hydrophobic surface is a thiol-modified hydrophobic surface.6. The device of claim 5 , wherein the thiol is a OH-thiol or a CH-thiol.7. The device of claim 1 , wherein the plurality of channels comprise a COOH-thiol-modified surface.8. The device of claim 1 , wherein the plurality of channels comprise a polystyrene-modified surface.9. The device of claim 1 , wherein at least one of the plurality of channels is a capillary.10. The ...

Chromatography columns and processes

A separation column for expanded bed adsorption comprises a column tube ( 2 ), a base ( 15 ) carrying an inlet rotor structure ( 6 ) for pumping in process liquid, and a top cap ( 3 ). The top cap ( 3 ) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange ( 22 ) of the column tube ( 2 ). The angle of the convergent interior surface ( 35 ) of the conical top cap ( 3 ) may be between 10 and 25°. A vortex-inhibitor component ( 8 ) projects down below the outlet structure ( 4 ) at the top of the cap, projecting into the operating space ( 15 ) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

METHOD AND SYSTEM FOR TRANSFERRING SEPARATION RESIN

A method and a system for transferring a slurry of a separation resin, from at least one first container to a second container, said method comprising the steps of: 1. A method for transferring a slurry of a separation resin , from at least one first container to a second container , said method comprising the steps of:connecting the at least one first container to the second container by a transferring connection;providing a degree of vacuum to the second container by a vacuum production device connected to the second container;allowing the vacuum in the second container to affect the content in the at least one first container through the transferring connection such that the slurry initially provided in the at least one first container is transferred to the second container through the transferring connection.2. A method according to claim 1 , wherein the step of connecting the at least one first container to the second container comprises connecting the two containers by disposable claim 1 , pre-sterilized connecting parts.3. A method according to claim 1 , wherein the step of allowing the vacuum in the second container to affect the content in the at least one first container comprises opening at least one valve provided in the transferring connection between the at least one first container and the second container.4. A method according to claim 1 , further comprising the step of:rinsing the at least one first container by transferring a rinsing solution through the at least one first container and further to the second container, said rinsing solution coming from a rinse bag connected to the at least one first container, said transferring of a rinsing solution being accomplished by the same vacuum as provided to the second container for transferring the slurry.5. A method according to claim 3 , wherein the step of rinsing the at least one first container comprises opening a rinsing valve provided in the connection between the at least one first container and ...

SINGLE USE SLURRYING AND CHROMATOGRAPHY SYSTEMS

Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration. 1. A disposable container for chromatography comprising:a flexible body configured to receive a chromatography resin, the flexible body comprising:a separation barrier that separates the flexible body into a filtration portion and a drainage portion;wherein the separation barrier retains the chromatography resin separately from the drainage portion during use while allowing fluids to pass therethrough.2. The disposable container of further comprising:at least one agitator disposed within the flexible body and configured to be raised and lowered therein.3. The disposable container of claim 2 , wherein the agitator in a raised position permits the chromatography resin to operate in a packed-bed configuration.4. The disposable container of claim 2 , wherein the agitator in a lowered position permits the chromatography resin to operate in a slurry configuration.5. The disposable container of further comprising:at least one probe coupled to the flexible body and operative to ...

TRANSITION ANALYSIS METHOD FOR CHROMATOGRAPHY COLUMN QUALIFICATION

The present disclosure is directed to a method of operating a chromatography column. This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is determined based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. The height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value. If during routine column monitoring, an adverse trend in HETP is observed or the control limits are exceeded, the eluate product quality, column process performance, and/or impurity removal data should be evaluated to ensure product quality for the identified batch. Should any of the product quality or column performance fail the criteria set, appropriate corrective action, such as conditioning, repacking or replacing the column, and qualification should be performed prior to release for further use. 117-. (canceled)19. The system of claim 18 , wherein the chromatography column is conditioned claim 18 , replaced claim 18 , or repacked based on said assessing.20. The system of claim 18 ,wherein the detector is configured to collect column outlet signals and accumulated flow parameters at two or more intervals of a corresponding mobile phase transition front during one or more subsequent uses of the chromatography column packing;wherein the computing device comprises processor and a non-transitory computer-readable medium with instructions stored thereon, which, when executed by the processor, perform steps comprising:a) said determining and said calculating using the column outlet signal and ...

HPLC COLUMN

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use. 137.-. (canceled)38. A chromatography column having a tubular body with an inner diameter , the column having opposing inlet and outlet ends , the column comprising:a bed of chromatographic media that has just been compressed to its maximum axial compression packing pressure during formation of the chromatographic bed;an upstream and downstream frit inside the tubular body and located on respective upstream and downstream ends of the bed of chromatographic media;a piston having opposing, upstream and downstream ends, the downstream end of the piston urging the upstream frit against the bed of chromatographic media and maintaining the axial compression packing pressure on the bed of chromatographic media, with no spring interposed between the first frit and the piston, the upstream end of the piston having an annular surface encircling a longitudinal axis of the column and facing away from the upstream frit;a fluid seal interposed between the piston and the inner diameter of the tubular body;a locking sleeve with multiple-parts that are connected together to encircle the inlet end of the column, each part of the locking sleeve having sleeve threads located thereon to form a threaded surface encircling the longitudinal axis;an annular locking collet having collet threads located to threadingly engage the ...

Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes

R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

METHOD OF PACKING CHROMATOGRAPHIC COLUMNS, PACKED CHROMATOGRAPHIC COLUMNS FOR USE AT HIGH PRESSURES AND USES THEREOF

A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure. 1. A method of packing a chromatography column , comprising:dispersing chromatographic media particles in an aqueous based slurry solution to form a slurry;filling a chromatography column with the chromatographic media particles by introducing the slurry to the column; andapplying a pressure to pack the chromatographic media particles in the chromatography column.2. The method of claim 1 , wherein the aqueous based slurry solution is 100% water claim 1 , or at least 90% claim 1 , or at least 95% claim 1 , or at least 99% claim 1 , by weight water.3. The method of claim 1 , wherein the aqueous based slurry solution contains organic solvent(s) in an amount not more than 100 parts by weight claim 1 , not more than 50 parts by weight claim 1 , or not more than 40 parts by weight claim 1 , or not more than 30 parts by weight claim 1 , or not more than 20 parts by weight claim 1 , or not more than 10 parts by weight claim 1 , or not more than 5 parts by weight claim 1 , or not more than 1 part by weight organic solvent(s) claim 1 , based on 100 parts by weight of water.4. The method of claim 1 , wherein the aqueous based slurry solution further comprises claim 1 , based on 100 parts by weight of water:0-50 parts by weight of surfactant;0-50 parts by weight of electrolyte or salt (other than ionic surfactant);0-100 ...

Liquid chromatography technique

LC techniques are disclosed. The LC technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150 degree Celsius, pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone.

Column packing material for supercritical fluid chromatography, column for supercritical fluid chromatography and preparation method therefor

A column packing material for supercritical fluid chromatography which allows good peak shape to be obtained in the analysis of free fatty acids, etc. and in the analysis of agricultural chemicals, etc., and has excellent durability for repeated analysis. The column packing material for supercritical fluid chromatography includes polymer particles containing cross-linked polymer, the degree of swelling of which after absorbing tetrahydrofuran and the degree of swelling of which after absorbing methanol are both 1.4 or less.

Method for Sanitization of a Chromatography Column

The invention discloses a method for sanitization of a bioprocess chro matography column, comprising the steps of: a) providing a bioprocess chromatogra phy column with a packed bed of separation resin particles and a movable adapter, b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet; c) raising the adapter to provide a gap between the packed bed and the adapter, d) lowering the adapter to close the gap; and e) conveying an equilibration liquid through the packed bed to a column outlet. 1. A method for sanitization of a bioprocess chromatography column , comprising the steps of:a) providing a bioprocess chromatography column with a packed bed of separation resin particles and a movable adapter,b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet;c) raising the adapter to provide a gap between the packed bed and the adapter,d) lowering the adapter to close the gap; ande) conveying an equilibration liquid through the packed bed to a column outlet.2. The method of claim 1 , further comprising claim 1 , between steps d) and e) claim 1 , a step d′) of conveying a sanitization fluid through the packed bed to a column outlet.3. The method of claim 1 , wherein steps c) and d) are repeated at least once claim 1 , such as twice.4. The method of claim 1 , further comprising claim 1 , after step b) and before step e) claim 1 , a step b′) of closing and opening a column resin valve.5. The method of claim 4 , wherein before step b′) claim 4 , said column resin valve has been equilibrated with sanitization fluid.6. The method of claim 1 , further comprising filling a flush channel between two adapter seals with sanitization fluid.7. The method of claim 1 , wherein said sanitization fluid comprises an oxidizing agent.8. The method of claim 7 , wherein said oxidizing agent comprises a peracid or hydrogen peroxide.9. The method of claim 7 ...

Methods for Chromatography Resin Slurry Determination

The present invention relates to methods for determining a slurry concentration for a slurry of a chromatography resin during a column packing. The present invention provides a more accurate and consistent method for determining a slurry concentration for packing a chromatography column. The method of the present invention utilizes an automated pump to provide a controlled flow rate to consolidate a resin sample. Based on the consolidated resin, a slurry concentration can be determined. The determined slurry concentration can be utilized to pack the resin in a chromatography column with highly accurate bed heights. 1. A method of determining a slurry concentration , comprising:adding a slurry comprising a resin to a first chromatography column;pumping a volume of liquid through the chromatography column at a constant flow rate to form a consolidated resin, wherein the volume of liquid is pumped through the chromatography column by an automated pump system;upon completion of the pumping step, measuring a bed height of the consolidated resin; anddetermining a slurry concentration from the measured bed height.2. The method of claim 1 , wherein the liquid is deionized water claim 1 , distilled water claim 1 , purified water claim 1 , a saline solution claim 1 , or an organic solvent.3. The method of claim 1 , wherein the step of adding the slurry further comprises adding water to the chromatography column and allowing the slurry to settle for about five minutes to about one hour prior to pumping.4. The method of claim 1 , further comprising utilizing the determined slurry concentration to pack a second chromatography column.5. The method of claim 4 , wherein the second chromatography column comprises a consolidation rate and wherein the flow rate is equivalent to the consolidation rate.6. The method of claim 1 , wherein the automated pump system comprises a syringe operatively coupled to a pump.7. A method for determining a slurry concentration for a slurry of a ...

CHROMATOGRAPHY COLUMN FRAME AND METHOD OF CONDUCTING MAINTENANCE ON AND PACKING OF A CHROMATOGRAPHY COLUMN

The invention relates to a chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder means connected to said support arrangement. Said holder means is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction. The invention also relates to method of conducting maintenance on and packing of a chromatography column. 1. A method of conducting maintenance on and packing of a chromatography column , the method comprising:providing a chromatography column comprising; a tubular housing;a first end unit removably connected to a first end of the tubular housing; a second end unit removably connected to a second end of the tubular an adaptor assembly moveable within said tubular housing; and 'an adaptor rod connected to said adaptor assembly, which adaptor rod is arranged to extend through an opening in the first end unit;', 'housing;'}providing a frame comprising at least two legs; 'disconnecting the first end unit or the second end unit from the tubular housing;', 'a support arrangement connected to said at least two legs; and a holder connected to said support arrangement;'} arranging a driver on the adaptor rod; and', 'activating the driver, so that the adaptor rod together with the first end unit and the adaptor assembly move vertically upwards separating the first end unit and the adaptor assembly from the tubular housing or also separating the tubular housing from the second end unit., 'positioning the adaptor rod in said holder, so that the adaptor rod is prevented from moving in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction;'}2. The method according to claim 1 , further comprising:performing maintenance on the chromatography column.3. The method according to claim 1 , further comprising:supplying a packing ...

ZWITTERIONIC COMPOUNDS AS GAS CHROMATOGRAPHIC COLUMN STATIONARY PHASES

A gas chromatographic (GC) column using a zwitterionic compound and methods of use thereof are disclosed herein. The volatile free acids were observed to strongly retain on these zwitterionic compounds-based columns with excellent peak symmetry. By carefully tuning the structures of these zwitterionic compounds, different selectivity toward volatile free acids was demonstrated. These stationary phases possess a wide working range with thermal stabilities at higher temperatures. 1. A method of chromatographic analysis or separation , the method comprises:separating an analyte by a column; wherein the column comprises a zwitterionic compound as its stationary phase.2. The method of claim 1 , wherein the column is a gas chromatographic (GC) column comprising a zwitterionic compound as its stationary phase claim 1 ,wherein the zwitterionic compound comprises one or more cationic systems and one or more anionic groups;{'sub': 3', '2, 'sup': −', '6', '−', '6, 'wherein the one or more anionic groups comprises at least a sulfonate, phosphonate, or a group containing a —SO or —P(OR)O group; where Ris H or substituted or unsubstituted alkyl group; and'}wherein the cationic system is a quaternary ammonium, phosphonium, sulfonium, guanidinium compound, or a positively charged heterocyclic group derived from a 5 or 6 membered heterocyclic group having at least one nitrogen or sulfur atom.3. The method of claim 1 , wherein the column is at a temperature from about 0° C. to about 120° C.7. The method of claim 6 , wherein the organic molecule is not derivatized before the sample is input into the GC column.8. The method of claim 6 , wherein the organic molecule is a C-C claim 6 , C-C claim 6 , C-C claim 6 , C-C claim 6 , C-Cester claim 6 , alcohol claim 6 , free acid claim 6 , ether claim 6 , aldehyde claim 6 , amine claim 6 , or mixture thereof and is volatile at room temperature or a temperature from about 0° C. to 50° C.9. The method of claim 6 , the method further comprises ...

Packing of Chromatography Columns

Disclosed herein is a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column. 1. A method for preparing a connected plurality of packed chromatography columns , comprising the steps of:a) providing dry chromatography medium particles and preparing a plurality of aliquots of said particles, which aliquots differ from each other by less than 5.0 percent by weight;b) providing a plurality of substantially identical chromatography columns and packing one of said aliquots into each chromatography column to obtain a plurality of packed chromatography columns; andc) fluidically connecting said plurality of packed chromatography columns in parallel.2. The method of claim 1 , wherein said aliquots differ from each other by less than 2.0 percent by weight claim 1 , such as by less than 1.0 percent by weight.3. The method of claim 1 , wherein in step a) said aliquots are prepared from a single homogeneous batch of dry chromatography medium particles.4. The method of claim 1 , further comprising claim 1 , after step a) and before step b) claim 1 , a step a′) of separately suspending said aliquots in a packing liquid.5. The method of claim 1 , further comprising claim 1 , after step b) or after step c) claim 1 , a step b′) of supplying a reswelling liquid to said plurality of packed chromatography columns.6. The method of claim 1 , wherein step c) further comprises fluidically connecting said plurality of chromatography columns to a single sample inlet.7. A method for manufacturing a chromatography subsystem comprising a plurality of chromatography columns connected in parallel claim 1 , said method ...

LIQUID CHROMATOGRAPHY TECHNIQUE

Liquid chromatography techniques are disclosed. Specifically, the liquid chromatography technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid-contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150° C., pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, and combinations thereof 1. A liquid chromatography technique , comprising:providing a liquid chromatography system having a coated stainless steel fluid-contacting element; andtransporting a fluid to contact the coated stainless steel fluid-contacting element;wherein the fluid includes adenosine triphosphate;wherein the coated stainless steel fluid-contacting element has a coating, the coating including carbon, silicon, oxygen, and hydrogen.2. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a frit.3. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a fitting.4. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a pump head.5. The technique of claim 1 , wherein the coated stainless steel fluid-contacting element is a valve.6. The technique of claim 1 , wherein the liquid chromatography system has a stationary phase claim 1 , the stationary phase having particles having a size of less than 6 micrometers.7. The technique of claim 1 , wherein the liquid chromatography system has a stationary phase claim 1 , the stationary phase having particles being hydrophobic.8. The technique of claim 1 , wherein the liquid chromatography system includes a solvent ...

Method and Apparatus for Packing a Chromatography Column Assembly

A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube () having a closed first end () comprising an inlet/outlet (), a media inlet () adjacent a second end of the column tube and an adaptor () positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume. 1. A method for packing a chromatography column assembly with chromatography media , comprising the steps of:providing: a column tube having a closed first end comprising an inlet/outlet; a media inlet adjacent a second end of the column tube; and an adaptor positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume;connecting a media slurry source to the media inlet;at least partially filling the internal volume with media slurry via the media inlet; andforcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.2. The method according to claim 1 , wherein the adaptor comprises an inlet/outlet.3. The method according to claim 1 , further comprising providing the system and ...

METHOD FOR MANUFACTURING A GAS PHASE CHROMATOGRAPHY COLUMN AND COLUMN OBTAINED USING SUCH A METHOD

The present invention relates to a method for manufacturing a chromatography column, in particular for a gas phase chromatography, comprising a stationary phase made from a sol. 1. A method for manufacturing a chromatography column comprising a stationary phase made from a sol comprising a pore-forming agent , comprising:(a) introducing a sol comprising a pore-forming agent at a first end of the column,(b) moving said sol towards a second end of the column, so that a sol layer is formed on the internal wall of the column wherein said sol layer forms a gel on the internal wall of the column, and(c) drying the gel, and(d) removing the pore-forming agent from the dried layer, so as to form a microporous, mesoporous layer or other porous layer, the size and/or the density of the pores being controlled, said porous layer forming the stationary phase.2. The method according to claim 1 , wherein the sol forms a plug claim 1 , extending from the first end of the column and over a length of less than two-thirds of the total length of the column claim 1 , the plug being moved along the column under the effect of a pressure.3. The method according to claim 1 , wherein (c) is carried out by circulating a gas inside the column and claim 1 , if required claim 1 , during (a).4. The method according to claim 2 , wherein the gas is air or helium claim 2 , nitrogen or another inert gas.5. The method according to claim 1 , wherein the pore-forming agent comprises cetyltrimethylammonium bromide (CTAB) claim 1 , diblock copolymers of ethylene oxide and of propylene oxide claim 1 , triblock copolymers of ethylene oxide and of propylene oxide claim 1 , or another surfactant.6. The method according to claim 1 , wherein (d) is carried out after (c) with a treatment selected from the group consisting of calcination claim 1 , washing with an organic solvent of the alcohol or acetone type claim 1 , and UV insolation.7. The method according to claim 6 , wherein the calcination is carried out by ...

Packing system and method for chromatography columns

The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.

CHROMATOGRAPHY COLUMN FRAME AND METHOD OF CONDUCTING MAINTENANCE ON AND PACKING OF A CHROMATOGRAPHY COLUMN

A chromatography column frame comprising at least two legs, a support arrangement connected to said at least two legs, and a holder connected to said support arrangement. Said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction. 1. A chromatography column frame , comprising:at least two legs;a support arrangement connected to said at least two legs; anda holder means connected to said support arrangement,wherein said holder is arranged to releasably hold an adaptor rod of a chromatography column, so that the adaptor rod is prevented to move in a horizontal direction and so the adaptor rod is allowed to move in a vertical direction.2. The frame according to claim 1 , wherein the holder comprises at least one support plate provided with a recess for the adaptor rod.3. The frame according to claim 1 , wherein the at least one support plate is provided with a bearing surface for a driver arranged on the adaptor rod.4. The frame according to claim 3 , wherein the driver is a threaded sleeve arranged to interact with threads on the adaptor rod claim 3 , so that the adaptor rod moves in the vertical direction when pivoting the sleeve.5. The frame according to claim 2 , wherein two support plates are arranged on the support arrangement claim 2 , each plate is provided with a recess claim 2 , which recesses together define an opening for the adaptor rod.6. The frame according to claim 5 , wherein at least one of the support plates are removably connected to the support arrangement.7. The frame according to claim 1 , further comprising a stopper arranged on the support arrangement claim 1 , wherein the stopper is adapted to interact with an axial groove in the adaptor rod claim 1 , so that the adaptor rod is prevented from pivoting in said holder.8. The frame according to claim 1 , wherein the support arrangement ...

CONDITIONING OF PACKED CHROMATOGRAPHY COLUMNS

The present invention relates to a method of conditioning a dry-packed chromatography column, which method comprises providing a column packed with inert and hydrophilic chromatography resin; and driving a solvent or solvent mix across the column. The solvent(s) are selected to be less hydrophilic than the chromatography resin; and the rate of the solvent flow is controlled to a rate which does not cause the system pressure to exceed a predefined threshold. The present invention is advantageously used in FLASH chromatography, where the generation of heat may pose problems e.g. to plastic frits or filters at high flow rates and/or wetting of dry resins. 2. A method according to claim 1 , wherein the one or more solvents provide an equilibration of the chromatography resin.3. A method according to claim 1 , wherein the passing the one or more solvents includes passing two column volumes of solvent across the column.4. A method according to claim 1 , wherein the resin comprises porous silica.5. A method according to claim 1 , whereinthe packed column comprises at least one filter or frit andthe at least one filter or frit is arranged downstream of the chromatography resin in the column.6. A method according to claim 5 , wherein the at least one filter or frit includes a heat-sensitive material.7. A method of controlling heat generation in a column claim 5 , the method comprising:passing a solvent across the column at a flow rate, such that a column pressure does not exceed a threshold,wherein the resin is more hydrophilic than the resin.8. A method according to claim 1 , wherein the control is performed by a computer.9. A system for conditioning a dry-packed chromatography column claim 1 , the system comprising:a column packed with at least one inert and hydrophilic chromatography resin;at least one solvent container;means for measuring pressure within the system;means for passing a solvent at an actively controlled flow rate across the column; anda computer configured ...

Liquid chromatography packing material

A liquid chromatography packing material employing crosslinked polymer particles, wherein the volume average particle size of the crosslinked polymer particles is at least 2 μm but not more than 10 μm, the amount of fine particles having a particle size of 1 μm or less among the crosslinked polymer particles is less than 1% by volume, and the identity coefficient in the particle size distribution is not more than 1.15.

USE OF VAPOR DEPOSITION COATED FLOW PATHS FOR IMPROVED ANALYTICAL ANALYSIS

A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I: 2. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of a polymeric material.3. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of glass.4. The sample preparation device of claim 1 , wherein a material forming the wetted surfaces of the fluidic path prior to coating are formed of metal.5. The sample preparation device of claim 1 , wherein the alkylsilyl coating has a contact angle of at least 15°.6. The sample preparation device of claim 5 , wherein the alkylsilyl coating has a contact angle of less than or equal to 60°7. The sample preparation device of claim 1 , wherein the alkylsilyl coating has a thickness of at least 100 Å.8. The sample preparation device of claim 1 , wherein the alkylsilyl coating of Formula I is bis(trichlorosilyl)ethane or bis(trimethoxysilyl)ethane.10. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula II is (3-glycidyloxypropyl)trimethoxysilane claim 9 , n-decyltrichlorosilane claim 9 , trimethylchlorosilane claim 9 , trimethyldimethyaminosilane claim 9 , methoxy-polyethyleneoxy(1-10) propyl trichlorosilane claim 9 , or methoxy-polyethyleneoxy (1-10) propyl trimethoxysilane.11. The sample preparation device of claim 10 , wherein the alkylsilyl coating of Formula II is (3-glycidyloxypropyl)trimethoxysilane followed by hydrolysis.12. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula I and II provides a desired contact angle of about 5° to about 60°.13. The sample preparation device of claim 9 , wherein the alkylsilyl coating of Formula I is bis(trichlorosilyl)ethane or bis ...

Method for Packing Chromatography Columns

The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns. 1. A method for packing a plurality of uniform chromatography columns , comprising the steps of:a) providing a plurality of chromatography columns;b) providing a plurality of chromatography resin aliquots;c) packing said chromatography resin aliquots in said chromatography columns to provide a plurality of packed chromatography columns; andd) subjecting said packed chromatography columns to repeated mechanical impacts.2. A method for packing a plurality of uniform chromatography columns , comprising the steps of:a) providing a plurality of chromatography columns;b) providing a plurality of chromatography resin aliquots;c) packing said chromatography resin aliquots in said chromatography columns to provide a plurality of packed chromatography columns; andd) subjecting said packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.3. The method of claim 1 , wherein said chromatography resin aliquots are dry and wherein in step c) said chromatography resin aliquots are filled in said chromatography columns and reswollen by adding a liquid to the chromatography columns.4. The method of claim 1 , wherein step b) comprises providing a plurality of dry chromatography resin aliquots and reswelling them with a liquid to provide a plurality of reswollen chromatography resin aliquots claim 1 , and wherein step c) comprises packing said reswollen chromatography resin aliquots in said chromatography columns.5. The method of ...

LIQUID CHROMATOGRAPHIC COLUMN AND LIQUID CHROMATOGRAPHIC APPARATUS INCLUDING THE SAME

A liquid chromatographic column includes: a cylindrical column body; an inflow-side filter that is disposed at an eluent inflow-side end of the column body; an outflow-side filter that is disposed at an eluent outflow-side end of the column body; and a filler that is filled between the inflow-side filter and the outflow-side filter, in which the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler, and the first resin filter member has an indentation elastic modulus lower than that of the second resin filter member. 1. A liquid chromatographic column , comprising:a cylindrical column body;an inflow-side filter that is disposed at an eluent inflow-side end of the column body;an outflow-side filter that is disposed at an eluent outflow-side end of the column body; anda filler that is filled between the inflow-side filter and the outflow-side filter,wherein the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler, andthe first resin filter member has an indentation elastic modulus lower than that of the second resin filter member.2. A liquid chromatographic column , comprising:a cylindrical column body;an inflow-side filter that is disposed at an eluent inflow-side end of the column body;an outflow-side filter that is disposed at an eluent outflow-side end of the column body; anda filler that is filled between the inflow-side filter and the outflow-side filter,wherein the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler,the first resin filter member is made of polyethylene, polypropylene, or polytetrafluoroethylene, andthe second resin filter member is made of polyether ether ketone or a ...

COLUMN PACKING APPARATUS AND PACKING METHOD

A column packing apparatus includes a tubular structure, a packing material supplier disposed on one end of the tubular structure for permitting a packing material slurry formed by a packing material dispersed in a solvent to be packed into the tubular structure, and a packing controller disposed on the other end of the tubular structure for determining the flow rate and pressure of the packing material slurry formed by the packing material being packed so that they can be maintained at predetermined flow rate and pressure level. A column packing method includes the steps of providing a packing controller disposed on the other end of the tubular structure, and permitting the packing controller to variably adjust the flow rate and pressure of the packing material slurry so that they can be maintained at the predetermined flow rate and pressure level. 1. A column packing apparatus wherein the column structure is designed for use in the chromatographic separation , the apparatus comprising:a tubular structure;a packing material supplier disposed on one end of said tubular structure for permitting a packing material slurry formed by a packing material dispersed in a solvent to be packed into said tubular structure; anda packing controller disposed on the other end of said tubular structure for maintaining the flow rate and pressure of said packing material slurry formed by said packing material being packed at predetermined flow rate and pressure level.2. A column packing apparatus as defined in claim 1 , wherein said packing controller includes:a resistive element for variably adjusting the discharging rate of said solvent being discharged from said other end of said tubular structure, wherein said resistive element is a resistor capable of variably adjusting the discharging rate of said solvent so that the flow rate and pressure of said packing material slurry can be maintained at said predetermined flow rate and pressure level.3. A column packing method wherein the ...

Laterally perfused chromatography element

A microfluidic chromatography element in which all components are situated in one plane and the mobile phase perfuses the stationary phase laterally.

Systems and methods for failure mode detection in process chromatography

The disclosure provides systems and methods useful for predicting or detecting a malfunction in a chromatography process in real-time. In some embodiments, the disclosure provides systems and methods for detecting an atypical profile in a process chromatogram in ion-exchange chromatography of a biologic product.

COMPREHENSIVE 2DGC SYSTEM COMPRISING OF A MODULATION COLUMN, A MODULATOR, AND A GAS CHROMATOGRAPH

A comprehensive two-dimensional gas chromatography system comprising of a sample inlet, a primary dimension column, a secondary dimension column, a thermal modulator, and a detector. The thermal modulator has a first modulation position and a second modulation position. The sample inlet connects to the primary dimension column. The exit of the primary dimension column connects to the first modulation position via a fluidic path, the first modulation position connects to the second modulation position via a fluidic path, the second modulation position connects to the secondary dimension column via a fluidic path, and the exit of the secondary dimension column connects to the detector. The fluidic path between the exit of the primary dimension column and the inlet of the secondary dimension column is a modulation column. 1. A modulation column used in a comprehensive two-dimensional gas chromatography system: the modulation column has only part of its length coated with a stationary phase. The modulation column is either an independent part from the primary dimension column and the secondary dimension column , or an integral part of the primary dimension column or the secondary dimension column.2. The modulation column of claim 1 , has a first and a second modulation positions claim 1 , wherein (1) only the first and second modulation positions have stationary phase coating; or (2) only the first modulation position has stationary phase coating; or (3) only the first modulation position and a distance extending to its upstream has stationary phase coating; or (4) only the first modulation position claim 1 , the second modulation position claim 1 , and the length between these two positions have stationary phase coating; or (5) only the first modulation position claim 1 , the second modulation position claim 1 , the length between these two positions claim 1 , and a distance extending to upstream of the first modulation position have stationary phase coating; or (6) ...

CHROMATOGRAPHY COLUMN SUPPORT

Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column. 2. The use according to claim 1 , characterized in that the legs are plates and that the connectors and the legs form a unit in which the upper edge of the respective leg is the connector.3. The use according to any one of the preceding claims claim 1 , characterized in that the legs are perforated plates.4. The use according to any one of the preceding claims claim 1 , characterized in that the support comprises one or more circular legs that have different radii with respect to each other and with respect to the axis of symmetry.5. The use according to any one of the preceding claims claim 1 , characterized in that a lateral flow between the sections defined by the legs of the support is possible.7. The support according to claim 6 , characterized in that the legs are plates and that the connectors and the legs form a unit in which the upper edge of the respective leg is the connector.8. The support according to any one of to claim 6 , characterized in that the legs are perforated plates.9. The support according to any one of to claim 6 , characterized in that the support comprises one or more circular legs that have different radii with respect to each other and with respect to the axis of symmetry.10. The support according to any one of to claim 6 , characterized in that a lateral flow between the sections defined by the legs of the support is possible.11. The ...

UNIFORMLY DENSE STATIONARY PHASE FOR CHROMATOGRAPHY

The present disclosure relates to a chromatographic stationary phase having a uniform polymer density, and related methods. In particular, the present disclosure relates to a method of forming a uniformly dense stationary phase inside a chromatography column. 1. A method of preparing a chromatography column , comprising(i) packing the chromatography column with support particles to form packed support particles within said column; and(ii) forming a polymer coating on the packed support particles.2. The method of claim 1 , wherein the support particles comprise silica claim 1 , alumina claim 1 , titania claim 1 , zirconia claim 1 , magnetite claim 1 , or combinations thereof.3. The method of claim 1 , wherein the support particles comprise an initiator for polymerization.4. The method of claim 1 , further comprising introducing a pre-polymer solution to the packed support particles.5. The method of claim 1 , wherein the step of forming a polymer coating comprises growing the polymer coating on the packed support particles.6. The method of claim 1 , wherein the step of farming a polymer comprises activators generated by electron transfer and atom-transfer radical polymerization.7. The method of claim 3 , wherein the step of forming a polymer comprises reversible addition fragmentation chain transfer claim 3 , and wherein the initiator is cyanomethyl [3-(trimethoxysilyl)propyl]trithiocarbonate.8. The method of claim 3 , wherein the step of forming a polymer atom-transfer radical polymerization claim 3 , and wherein the initiator is ((chloromethypphenylethyl)trimethoxysilane claim 3 , 3-trimethoxysilyl) propyl 2-bromo 2-methylpropionate claim 3 , [11-(2-bromo-2-methyl)propionyloxy]undecyltrichlorosilane or combinations thereof.9. The method of claim 1 , wherein the support particles comprise an initiator on a surface of the support claim 1 , and wherein the step of forming a polymer coating comprises growing the polymer coating on the surface of the packed support ...

LIQUID CHROMATOGRAPHY BASED DETECTION AND QUANTITATION OF PHOSPHO PRODRUGS AND THEIR ACTIVE METABOLITES

The present disclosure relates to the use of vapor deposition coated flow paths for improved chromatography and sample analysis using liquid chromatography-mass spectrometry (LC/MS) or liquid chromatography-optical detection (LC/UV). More specifically, this technology relates to separating and quantitation of analytes (e.g., phospho prodrugs and its phosphorylated metabolites) from a sample matrix (e.g., mammalian blood, plasma) using chromatographic devices and fluidic systems having coated flow paths. The LC-MS or LC-UV techniques provide improved recovery, peak shape and dynamic range in the analysis of the prodrug and its metabolites. 1. A method of detecting remdesivir in a sample , the method comprising:providing a sample to a chromatography column housing a mixed-mode stationary phase disposed therein, the chromatography column comprising an alkylsilyl coating covering at least a portion of wetted internal surfaces of the chromatography column;separating and eluting remdesivir from the sample by applying a gradient of a mobile phase solution comprising ammonium acetate; anddetecting remdesivir in the eluent using a mass spectrometry detector or an optical detector.2. The method of claim 1 , wherein the mobile phase solution does not include an ion pairing reagent.3. The method of claim 1 , wherein the mobile phase solution has a pH within the range of 4.8 to 7.4. The method of claim 1 , further comprising detecting one or more phosphorylated metabolites of remdesivir in the eluent using the mass spectrometry detector or the optical detector.5. The method of claim 1 , wherein the gradient is a linear gradient.6. The method of claim 1 , wherein the gradient is achieved by varying a concentration of ammonium acetate.7. The method of claim 6 , wherein the gradient is achieved by varying a concentration of acetonitrile.8. The method of claim 7 , wherein the concentration of acetonitrile ranges from 0 to 60 volume percent.9. The method of claim 1 , wherein the ...

Chromatography Column Packing Medium Recovery

Systems include a chromatography column tube having an inlet and outlet and port assembly arranged in a wall of the column tube between flow distributors that together form a chamber within the tube that is filled with packing medium are described. The port assembly facilitates the removal of resin from the pre-packed column and the port assembly does not affect fluid flow in the normal use of the column for chromatographic separation or ability to maintain sanitary conditions within the column. Also described are methods that include attaching tubing to a pump and to a column inlet and a column outlet, opening the port assembly, attaching tubing to the port assembly and to a second reservoir, and pumping aqueous solution from a reservoir into the chamber and out through the port assembly into the second reservoir, thereby removing packing medium from the column along with the flowing aqueous solution. 1. A method of recovering packing medium from a pre-packed chromatography column comprising a column tube having an inlet and an outlet and a port assembly arranged in a wall of the column tube between first and second flow distributors that together form a chamber within the column tube that is filled with packing medium , the method comprising:attaching tubing from a first reservoir of aqueous solution to a pump and to the column inlet and the column outlet;opening the port assembly;attaching tubing to the port assembly and to a second reservoir; andpumping the aqueous solution from the first reservoir into the column inlet and column outlet into the chamber and out through the port assembly into the second reservoir, thereby removing packing medium from the column along with the flowing aqueous solution.2. The method of claim 1 , wherein opening the port assembly comprises removing a sanitary clamp from the port assembly.3. The method of any one of to claim 1 , wherein the second reservoir is the same as the first reservoir.4. The method of any one of to claim 1 , ...

CENTRIFUGAL-DRIVEN MICROFLUIDIC PLATFORM AND METHOD OF USE THEREOF

In this invention, chromatography is integrated on a centrifugal platform to enable low-cost automated purification. Differing from the traditional chromatography method, purification and separation of a centrifugal compound collecting platform disclosed in the present invention mainly uses a centrifugal force to drive the fluid to flow outward in the radial direction when the motor rotates. The compounds to be separated react with the column packing during the flow, and the compounds with different polarities in the sample are gradually separated. The flow of the fluid can be governed by the motor and the geometry of the fluidic design such that compounds with different characteristics can be separated and collected in different collecting chambers. 1. A centrifugal-driven microfluidic platform , comprising:a microfluidic module, comprising a microfluidic disk and a microfluidic structure; at least one injection chamber module, disposed on the microfluidic disk;', 'at least one separation column, disposed on the microfluidic disk and connected with the injection chamber module;', 'at least one dispensing channel, disposed on the microfluidic disk and connected with the separation column by a connection channel module;', 'a plurality of collection chambers, disposed on the microfluidic disk; wherein each of the collection chamber connects with the dispensing channel respectively;', 'a waste chamber, disposed at the end of the dispensing channel;, 'wherein the microfluidic structure further comprisesa driving module, wherein a microfluidic module is disposed on the driving module;an operation module, wherein the operation module drives the driving module; anda detection module, wherein the detection module connects with the microfluidic module and the driving module respectively;wherein at least one separation column is spiral shaped;wherein the at least one dispensing channel is spiral structure or arc shaped.2. The centrifugal-driven microfluidic platform as ...

Chromatography system with tilt-prevention structure and associated process

Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube has a process fluid input device at the bottom and a movable piston in the top. The piston is enclosed in the column by a cover plate. The piston body has an inflatable seal, and is connected by a frame to a contact ring which carries another inflatable member to contact the tube wall. Process fluid leaves the operating volume through an opening of the piston and flexible hose, through the enclosed space and out through the cover plate. The space above the piston can be pressurised to control piston movement. The contact ring maintains piston alignment. The inflatable seals are used to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound sensors.

CONTAINERS FOR CHROMATOGRAPHY MEDIA

The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use. 1. A method of packing a chromatography column , said method comprising the steps ofa. inserting the flexible bag for chromatographic medium of comprising: an exterior wall of a non-porous material attached to both a first liquid distribution element and a second liquid distribution element at respective opposed ends of said exterior wall_thereby defining a compartment for chromatographic medium therein; said first liquid distribution element facing said second liquid distribution element, the first liquid distribution element being welded or moulded to the exterior wall; a medium filling port; and a first end piece and a second end piece attached to said exterior wall and adjacent to the first liquid distribution element and the second liquid distribution element respectively, the first end piece and the second end piece each comprising an opening for receipt of an inlet or an outlet for carrier liquid_and wherein the first liquid distribution element and the second liquid distribution element is each selected from the group consisting of filter, mesh, net, and sinter, and each of the first and second liquid distribution elements includes pores that are porous to liquids and do not allow the chromatographic medium to pass through into a chamber of a chromatography column;b. closing the column housing; andc. forming or compressing a packed bed or a consolidated bed or a fluidised bed of chromatographic medium.2. The method of claim 1 , wherein said bag contains dry chromatographic medium and said packed bed is formed by adding liquid to swell said medium to give a swollen volume Vs in a liquid of about 105-120% of the column chamber.3. The method of claim 1 , ...

LC-Column with Dynamic-Axial Compression (DAC)

A separating column for use with a filling tube to prepare for chromatography is disclosed. The separating column includes an axially movable spring stamp; and a plurality of springs coupled to the stamp. Moreover, the stamp is depressed directly into the column tube during column packing via the filling tube. Additionally, the stamp is fixed in the column tube while maintaining the packing pressure so that the springs are compressed during the packing process and press the stamp permanently and dynamically onto a chromatographic bed. 1. A separating column for use with a filling tube to prepare for chromatography , the separating column comprising:an axially movable spring stamp; anda plurality of springs coupled to the stamp,the stamp being depressed directly into the column tube during column packing via the filling tube; andthe stamp being fixed in the column tube while maintaining the packing pressure so that the springs are compressed during the packing process and press the stamp permanently and dynamically onto a chromatographic bed.2. The separating column according to claim 1 , wherein the plurality of springs hold the stamp in a fixed position inside the column tube.3. The separating column according to claim 1 , wherein the plurality of springs are comprised of stainless steel.4. The separating column according to claim 1 , further comprising a plurality suspension elements backing the stamp.5. The separating column according to claim 1 , wherein a DAC separating column is removed from the filling tube after a packing process.6. The separating column according to claim 2 , further comprising a plurality of spacers that comprise a plurality of half shells holding the spring stamp in the fixed position.7. A liquid chromatography column for use with a filling tube containing a particle-liquid mixture and a packing apparatus pressing the particle-liquid mixture from the filling tube into the liquid chromatography column claim 2 , the liquid chromatography ...

Chromatography column and method of conducting maintenance on a chromatography column

A chromatography column including: a tubular housing; a first end unit removably connected to a first end of the tubular housing; a second end unit removably connected to a second end of the tubular housing; an adaptor assembly movable within said tubular housing; an adaptor rod connected to said adaptor assembly, which adaptor rod is arranged to extend through an opening in the first end unit; a filter removably connected to the adaptor assembly; and a frame connected to said tubular housing for supporting the column on a floor. At least an actuating means is arranged on the frame, so that the column can be lifted and lowered in a substantially vertical direction.

METHOD OF AND DEVICE FOR PACKING A CHROMATOGRAPHY COLUMN

A method and device for packing a chromatography column formed of one or more vibration devices attached to top and/or bottom flanges of the column. Media is added in one or more steps to the column, allowed to settle under the effects of gravity and then subjected to one or more treatments of vibration from the vibration devices until a suitably packed column is obtained. Liquid used to suspend the media while being placed into the column may be at least partially removed before or during the vibration step(s). The remaining liquid is then removed or replaced after the packing has been obtained. 1. A method of packing a chromatography column comprising the steps of:a. providing a chromatography column having one or more vibration devices attached to its outer surface;b. forming a slurry with a media and a buffer solution;c. transferring the slurry into the column and immediately applying flow for 1 minute to settle the slurry in the column;d. activating the one or more vibration devices to generate vibrations for 1 minute following the settling of the slurry under flow for 1 minute;e. repeating flow for 1 minute followed by vibrations for 1 minute, until a packed bed of media is obtained.2. The method of claim 1 , wherein the media comprises controlled pore glass with a Protein A ligand.3. The method of claim 1 , wherein the buffer solution is present from about 10 to about 90% of the volume of the slurry.4. The method of claim 2 , wherein the controlled pore glass media has a 100 μm diameter and pore size of 1000A.5. The method of claim 1 , wherein the column comprises two or more vibration devices on the outer surface of the column.6. The method of claim 5 , wherein the two or more vibration devices are evenly spaced apart on the outer surface of the column.7. The method of claim 1 , wherein the column comprises three or more vibration devices on the outer surface of the column.8. The method of claim 7 , wherein the three or more vibration devices are evenly ...

Chromatography column qualification in manufacturing methods for producing anti-il12/il23 antibody compositions

A method of operating a chromatography column is described for use in methods of manufacture for producing anti-IL-12/IL-23p40 antibodies, e.g., the anti-IL-12/IL-23p40 antibody STELARA® (ustekinumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value.

CHROMATOGRAPHY COLUMN QUALIFICATION IN MANUFACTURING METHODS FOR PRODUCING ANTI-TNF ANTIBODY COMPOSITIONS

A method of operating a chromatography column is described for use in methods of manufacture for producing anti-TNF antibodies, e.g., the anti-TNFα antibody SIMPONI® (golimumab). This method involves collecting column outlet signal and accumulated flow parameters at two or more intervals of at least one mobile phase transition front during operation of the chromatography column comprising column packing. A model gamma cumulative distribution curve is calculated based on the collected column outlet signal and accumulated flow parameters for the at least one mobile phase transition front. A height equivalent theoretical plate (HETP) value is calculated for the at least one mobile phase transition front using parameters of the model gamma cumulative distribution curve and the quality of the chromatography column packing is assessed based on the calculated HETP value. 2. The method of claim 1 , further comprising:conditioning, replacing, or repacking the chromatography column based on said assessing.3. The method of further comprising:collecting column outlet signal and accumulated flow parameters at two or more intervals of a corresponding mobile phase transition front during one or more subsequent uses of the chromatography column packing;performing said determining and said calculating using the column outlet signal and accumulated flow parameters collected during each of the one or more subsequent uses of the chromatography column packing;determining an HETP value of the chromatography column packing during each of said one or more subsequent uses based on said performing;compiling a trend of the determined HETP values of the chromatography column packing of the two or more subsequent uses; andidentifying a change in the quality of the chromatography column packing based on said compiled trend, wherein said conditioning, replacing or repacking the chromatography column is based on said identifying.4. The method of claim 3 , wherein an increase in the HETP value of ...

CRESCENT PLOT COLUMNS AND METHODS FOR PREPARING CRESCENT PLOT COLUMNS

A crescent PLOT column is disclosed, including a capillary column having an inlet, an outlet, a bore, and an inner surface surrounding the bore and extending between the inlet and the outlet. A layer of particles is localized on a radial portion of the inner surface. The layer of the particles includes a radial thickness decreasing from a center of the radial portion to a periphery of the radial portion, forming a crescent shape in a radial frame of reference. A method for preparing the crescent PLOT column is disclosed, including loading the capillary column with a fluid including a carrier and particles such that the fluid is contained within the capillary column. The capillary column and the fluid contained within the capillary column are subjected to a centrifugal force. The carrier is removed, and a layer of the particles is localized on the radial portion of the inner surface. 1. A crescent porous layer open tubular (“PLOT”) column , comprising: an inlet;', 'an outlet;', 'a bore; and', 'an inner surface, the inner surface surrounding the bore and extending between the inlet and the outlet; and, 'a capillary column, the capillary column includinga layer of particles localized on a radial portion of the inner surface, the layer of the particles including a radial thickness decreasing from a center of the radial portion to a periphery of the radial portion, forming a crescent shape in a radial frame of reference.2. The crescent PLOT column of claim 1 , wherein the layer of the particles is essentially uniform along an axial length of the inner surface.3. The crescent PLOT column of claim 2 , wherein the layer of the particles is uniform along the axial length of the inner surface.4. The crescent PLOT column of claim 1 , wherein the capillary column includes a capillary material claim 1 , and the capillary material includes fused silica.5. The crescent PLOT column of claim 1 , wherein the capillary column includes a capillary material claim 1 , and the capillary ...

DETECTION OF A SUSPECT COUNTERFEIT PART BY CHROMATOGRAPHY

Parts are exposed to liquid chromatography to generate a corresponding chromatogram, wherein the chromatogram is compared to a chromatogram of a genuine part to determine if the tested part is suspect counterfeit. Depending on the selected predetermined target analytes, the generated chromatogram can be used to assess an associated manufacturing process as conforming or non-conforming. 1. A method of assessing a suspect part , the method comprising:(a) exposing a portion of a suspect part to a solvent by contacting a sealing interface with a surface of the suspect part to form a reservoir volume partly defined by the portion of the suspect part and disposing a solvent in the reservoir volume to contact the portion of the suspect part partly defining the reservoir volume, the solvent configured to dissolve at least one predetermined target analyte;(b) generating a first chromatogram corresponding to the at least one predetermined analyte dissolved in the solvent exposed to the portion of the suspect part; and(c) comparing the first chromatogram to a preexisting chromatogram corresponding to at least one of a known genuine part and a conforming process to output an indicator, the indicator corresponding to a level of the predetermined analyte relative to a predetermined threshold, wherein the indicator provides for assessing the suspect part.2. The method of claim 1 , further comprising one of rejecting or accepting the suspect part based on the indicator.3. The method of claim 1 , wherein the at least one predetermined target analyte includes at least one target anion and one target cation.4. The method of claim 1 , wherein the at least one predetermined target analyte includes at least three target anions and three target cations.5. The method of claim 1 , further comprising adjusting a volume of the solvent corresponding to a volume of the suspect part.6. The method of claim 1 , further comprising determining a status of the suspect part as at least one of a ...

ZWITTERIONIC COMPOUNDS AS GAS CHROMATOGRAPHIC COLUMN STATIONARY PHASES

A gas chromatographic (GC) column using a zwitterionic compound and methods of use thereof are disclosed herein. The volatile free acids were observed to strongly retain on these zwitterionic compounds-based columns with excellent peak symmetry. By carefully tuning the structures of these zwitterionic compounds, different selectivity toward volatile free acids was demonstrated. These stationary phases possess a wide working range with thermal stabilities at higher temperatures. 1. A gas chromatographic column comprising a zwitterionic compound as its stationary phase ,wherein the zwitterionic compound comprises one or more cationic systems and one or more anionic groups;{'sub': 3', '2, 'sup': −', '6', '−', '6, 'wherein the one or more anionic groups comprises at least a sulfonate, phosphonate, or a group containing a —SO or —P(OR)O group; where Ris H or substituted or unsubstituted alkyl group; and'}wherein the cationic system is a quaternary ammonium, phosphonium, sulfonium, guanidinium compound, or a positively charged heterocyclic group derived from a 5 or 6 membered heterocyclic group having at least one nitrogen or sulfur atom.3. The gas chromatographic column of claim 2 , wherein one of Rand Ris a sulfonate claim 2 , phosphonate claim 2 , or a group containing a —SO or —P(OR)O group; where Ris H or substituted unsubstituted alkyl group; and another of Rand Ror one of R claim 2 , R claim 2 , and Ris an alkyl group.5. The column of claim 1 , wherein the zwitterionic compound is a neutral or charged.6. The column of claim 1 , wherein the zwitterionic compound is a liquid at a temperature between about 0° C. and 100° C.7. The column of claim 2 , wherein Ris a straight or branched C-Cnonionic alkyl group and Ris a straight or branched C-Calkyl sulfonate claim 2 , phosphonate claim 2 , or group containing a —SO claim 2 , or —P(OR)O group; where Ris H or substituted or unsubstituted alkyl group.8. The column of claim 2 , wherein at least one of Rand Ris a C- ...

PACKAGE FOR BATCH CHROMATOGRAPHY

A method for separating at least one target compound from a feed solution is provided. The method includes filling a bioprocess package with a chromatography resin. The bioprocess package includes a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet. The method further includes flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin, washing the chromatography resin in the bioprocess package, and eluting the chromatography resin. 1. A method for separating at least one target compound from a feed solution , the method comprising: a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container; and', 'a channel-forming feature in the interior compartment of the container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet;, 'filling a bioprocess package with a chromatography resin, the bioprocess package comprisingflowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin;washing the chromatography resin in the bioprocess package; andeluting the chromatography resin such that substantially all of the at least one target compound is released from the chromatography resin.2. The method of claim 1 , wherein filling a bioprocess ...

Chromatography Column Comprising an Internal Bracing

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber. 1. A chromatography column for bioprocess separations , said column comprising:a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen;b) a first end wall, secured to or integral with said at least one side wall, with a first port fluidically connected via a first distributor to said first bed support screen;c) a second end wall, secured to or integral with said at least one side wall, with a second port fluidically connected via a second distributor to said second bed support screen;d) a packing port in one of said at least one side wall and first and second end walls; ande) an internal bracing secured to, or integral with, at least one of said first and second end walls and extending into said bed chamber.2. The chromatography column of claim 1 , wherein at least part of said internal bracing spans the entire axial height of the bed chamber claim 1 , being secured to claim 1 , or integral with claim 1 , both of the first and second end walls.3. The chromatography column of claim 1 , wherein said internal bracing comprises one or more rods claim 1 , each having a first rod end secured to claim 1 , or integral with claim 1 , said first end wall and a second rod end secured to claim 1 , or integral with claim 1 , said second end wall.4. The ...

Superficially porous supports for chromatography

Separating agent for chromatography and process for producing the same

상기 다당류에 존재하는 수산기 내의 일부의 수산기가 서로 가교 분자를 통해 가교되어 있고, 상기 다당류에 존재하는 수산기 내의 가교되어 있지 않은 수산기는 수식 분자에 의해 수식된 구조로 되어 있는 다당류 유도체를, 담체에 담지하지 않고, 예를 들면 비드 형상으로 성형시킨 크로마토그래피용 분리제에 관한 것이다. 본 발명에 의하면, 용출 용매에 의한 용출의 우려가 적고, 한번에 광학 분할을 할 수 있는 양이 크고, 큰 압력에 견딜 수 있는 것이 가능한 크로마토그래피용 분리제를 제공할 수 있다. Some of the hydroxyl groups in the hydroxyl group present in the polysaccharide are crosslinked with each other via a crosslinking molecule, and the uncrosslinked hydroxyl group in the hydroxyl group present in the polysaccharide carries a polysaccharide derivative having a structure modified by a modified molecule on a carrier. Instead, the present invention relates to a separation agent for chromatography molded into a bead shape, for example. Advantageous Effects of Invention The present invention can provide a separation agent for chromatography that is less likely to be eluted by an eluting solvent, has a large amount capable of optical division at one time, and can withstand a large pressure. 크로마토그래피용 분리제, 광학 분할, 다당류 유도체 Separators for Chromatography, Optical Fractionation, Polysaccharide Derivatives

色谱分离剂及其生产方法

色谱分离剂，它包括具有其中存在于多糖中的一部分羟基通过交联分子彼此交联和存在于多糖中的未交联羟基各自用改性分子改性的结构的多糖衍生物。该多糖衍生物不是以担载于载体的状态存在，并且已经成形为例如珠粒。该分离剂在洗脱溶剂中不容易溶解，可以一次光学离析大量化合物。它能够耐受高压。

Method of filling columns

Изобретение позвол ет ускорить процесс заполнени  хроматографичес- ких колонок дисперсным сорбентом путем подачи пневмоимпульсов в процессе засыпани  и уплотнени  сорбента попеременно с обоих концов колонки с контролем степени заполнени  в процессе уплотнени . 1 ил. The invention makes it possible to accelerate the process of filling the chromatographic columns with a dispersed sorbent by supplying pneumatic pulses in the process of backfilling and compacting the sorbent alternately at both ends of the column with control of the degree of filling in the compaction process. 1 il.

将可收缩支撑料用于多孔整体材料的方法和设备

本发明提供了一种加工制品和装置及其形成和使用方法，其中所述制品包括容纳在无机材料制成的支承体中或由该支承体限制边界的多孔无机基材，其中所述多孔基材和支承体被加热到一定温度，使支承体有效地收缩到多孔基材上，从而在多孔基材与支承体之间形成液密封接触。在一个优选方面，多孔无机基材的总孔隙率至少为5％，并且是用溶胶－凝胶法形成的多孔整体料。这样形成的制品提供了通过多孔基材的受限流体流，在分离、催化、过滤等方面提供了优异的性能。

Method and apparatus for forming an homogeneous mixture of chromatography media in a vessel

The present invention relates to methods and apparatus for forming an homogeneous mixture of chromatography media in a vessel. The invention also relates to methods and apparatus for transferring the homogeneous media from the vessel into a second vessel in preparation for packing a chromatography column. The invention can also be used directly to pack the column with homogeneous media.

Zeolitic capillary columns for gas chromatography

Methods have been found to prepare fused silica capillary gas chromatographic columns where the stationary phase is a molecular sieve affixed to the silica capillary wall without the aid of an organic binder by modifying the interior surface of the silica prior to contact with the molecular sieve. These totally inorganic columns greatly expand the application and range of gas chromatographic separations and allow the use of non-traditional carrier gases, even air, while not degrading the separation of components. The columns are films of small molecular sieve particles affixed to a silica surface modified by such treatments as hydrogen peroxide, alumina deposition, or silica deposition followed by fixation at 80°-160° C.

Patent FR2522154B1

Micro column reactor

Mikrosäulenreaktor zur Durchführung von Reaktionen an festen Phasen und/oder mit biologischen Zellen bestehend aus mindestens einem ersten und einem zweiten Substratplättchen (1; 2), die miteinander flächig verbunden sind, wobei in wenigstens eins der Substratplättchen (1; 2) wenigstens ein längserstreckter Kanal (3) eingebracht ist, der in einem vorgebbaren Abschnitt (a) seiner Länge von zwei durch das Substratplättchen geführten Durchtrittsöffnungen (41; 42) für den Zulauf von Substrat bzw. Ablauf von Produkt erfasst ist, wobei die Durchtrittsöffnungen (41; 42) vom Kanal (3) durch eine teildurchlässige siebartige Membran (5; 50) getrennt sind, deren Durchlassbereiche (51) so bemessen sind, dass sie in den Kanal (3) eingebrachte Mikroperlen und/oder Zellen (7) vorgebbar am Eintritt in die Durchtrittsöffnungen (41; 42) hindern, und dass der Kanal (3), außerhalb des durch die Durchtrittsöffnungen (41; 42) erfassten Abschnitts (a), mit wenigstens zwei weiteren Öffnungen (61; 62) versehen ist und Mittel (92) zum zeitweisen Verschluss wenigstens einer der Durchtrittsöffnungen...

Apparatus and method for trapping bead based reagents within microfluidic analysis systems

The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.

Apparatus and method for trapping bead based reagents within microfluidic analysis system

The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.

CHROMATO COLUMN HOLDER

1. Применение держателя для хроматографической колонки для поддержки хроматографического материала в хроматографической колонке, при этом указанный держатель для хроматографической колонки изготовлен из нержавеющей стали, или силикона, или политетрафторэтилена, или стекла и включает- по меньшей мере одну плоскость симметрии,- одну ось симметрии,- по меньшей мере три опоры,- по меньшей мере три прямых соединительных элемента,при этом указанные соединительные элементы образуют плоскость, перпендикулярную оси симметрии держателя,при этом указанные соединительные элементы соединены друг с другом на или вокруг оси симметрии,при этом каждая опора прикреплена к соединительному элементу, при этом каждая опора перпендикулярна плоскости, образуемой соединительными элементами,при этом все опоры расположены на одной стороне плоскости, образуемой соединительными элементами.2. Применение по п.1, отличающееся тем, что указанные опоры представляют собой пластины, и тем, что указанные соединительные элементы и опоры образуют единый блок, в котором верхняя грань соответствующей опоры представляет собой соединительный элемент.3. Применение по п.1, отличающееся тем, что указанные, опоры представляют собой перфорированные пластины.4. Применение по п.1, отличающееся тем, что держатель включает одну или более кольцевых опор, имеющих различные радиусы по отношению друг к другу и по отношению к оси симметрии.5. Применение по п.1, отличающееся тем, что возможен латеральный поток между отсеками, образуемыми опорами держателя.6. Держатель для хроматографической колонки, включающей- по меньшей мере одну плоскость симметрии,- одну ос РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (51) МПК G01N 30/60 (13) 2013 138 115 A (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2013138115/28, 31.01.2012 (71) Заявитель(и): Ф. ХОФФМАНН-ЛЯ РОШ АГ (CH) Приоритет(ы): (30) Конвенционный приоритет: (72) Автор(ы): БЛАШИК Андреас (DE) 02.02.2011 EP 11153063.0 (85) Дата ...

Adapter for changing the height of the gel bed in a chromatographic separation column

System for filling column of high performance liquid chromatography

The present invention relates to a high performance liquid chromatography column filling system. More specifically, provided is a high performance liquid chromatography column filling system which comprises: a tank part storing a solvent; a pump including an inlet and an outlet and filling the column with the solvent through the outlet by driving a cam; and a controlling part taking control to linearly raising filling pressure for the solvent by the pump in proportion to a time.

Preparation method and application of load-controllable strong-cation solid-phase extraction filler

本发明涉及一种担载量可控的强阳离子固相萃取填料的制备方法及应用，其结构式如图。以含有苯环结构的聚合物为反应物，选择不同的磺化剂，采用表面修饰法一步制备固相萃取填料。该固相萃取填料具有担载量可控、比表面积大、富集效率高、制备简单和节约成本等优点。本发明制备的固相萃取填料适用于动物源食品中β‑受体激动剂的检测前处理工作，具有良好的经济和社会效益。 图1一种强阳离子固相萃取填料的结构示意图其中，

Preparation and application of hydrogel @ silicon dioxide liquid chromatography filler

本发明提供了一种水凝胶@二氧化硅液相色谱填料的制备方法，是首先在介孔二氧化硅表面涂覆一层由N,N‑二甲基丙烯酰胺形成的水凝胶，然后再在粘性涂层表面涂覆一层由N,N‑二甲基丙烯酰胺和正十八烯形成的水凝胶层而得。本发明在硅胶小球表面通过物理涂覆和化学涂覆相结合的制备技术和溶剂蒸发法的后处理技术制备而得，合成路径简单易操作，由于水凝胶中的酰胺键和十八烷基的存在，使得该色谱填料具有很好的色谱分离性能，尤其是在亲水相互作用模式下，对极性分析物核苷碱基、苯甲酸类化合物、磺胺类药物、氨基酸、糖类化合物都具有较好的分离选择性。

Chromatography columns with continuous beds formed in situ from aqueous solutions

A chromatography column with a continuous solid bed spanning the cross section of the column, the bed containing channels large enough for hydrodynamic flow, is prepared by polymerizing a mixture of monomers and ammonium sulfate in an aqueous solution. The monomers include a monofunctional monomer such as a vinyl, allyl, acrylic, or methacrylic compound, and a polyfunctional monomer (i.e., a crosslinker), the total monomer concentration being in the range of 10% to 20% by weight, the mole ratio of crosslinker to total monomer being in the range of 0.3 to 0.4, and the ammonium sulfate having a concentration in the range of 0.4 M to 0.8 M. Functional groups to impart specialized separation capabilities, notably anion and cation exchange, can be included in the monomer mixture.

Gas chromatographic separating material

Method of manufacturing a metal column

본 발명의 실시예에 따른 금속 컬럼을 제조하는 방법을 제공한다. 3D 프린팅 기술을 이용하여 금속 컬럼을 제조하는 방법은 상기 금속 컬럼을 프린팅 하기 위한 3D-CAD 디자인을 생성하는 단계, 상기 금속 컬럼을 프린팅하는 단계, 상기 금속 컬럼의 채널 내부 표면을 저온에서 전 처리하는 단계 및 혼합 기체를 분리할 수 있도록 상기 채널 내부 표면을 정지상으로 코팅하는 단계를 포함한다.

Holder for chromatographic column

FIELD: instrument making. SUBSTANCE: present invention disclosed is holder for chromatographic column, which enables to establish movable frit inside chromatographic column in desired position. Holder for chromatographic column includes at least one plane of symmetry, one axis of symmetry, at least three supports, at least three straight connecting elements. At that, said connection elements form plane perpendicular to axis of symmetry of holder. At that, said connection elements are connected to each other on axis of symmetry. Each support is connected to connection element, and each support is perpendicular to plane formed by connecting elements, wherein all supports are located on one side of plane formed by connecting elements for stabilization of filler chromatographic column. EFFECT: providing stability and homogeneity of filling of chromatographic material from inside, providing possibility of varying and selecting height of holder and together with height of corresponding horizontal segment of chromatographic column in accordance with properties of chromatographic material. 15 cl, 8 dwg РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (51) МПК G01N 30/60 (13) 2 584 176 C2 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ (21)(22) Заявка: ИЗОБРЕТЕНИЯ К ПАТЕНТУ 2013138115/28, 31.01.2012 (24) Дата начала отсчета срока действия патента: 31.01.2012 (72) Автор(ы): БЛАШИК Андреас (DE) (73) Патентообладатель(и): Ф. ХОФФМАНН-ЛЯ РОШ АГ (CH) Приоритет(ы): (30) Конвенционный приоритет: (43) Дата публикации заявки: 10.03.2015 Бюл. № 7 R U 02.02.2011 EP 11153063.0 (45) Опубликовано: 20.05.2016 Бюл. № 14 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 02.09.2013 2 5 8 4 1 7 6 (56) Список документов, цитированных в отчете о поиске: JP 62042051 A 24.02.1987 . US 2010276370 A1 04.11.2010 . JP 2008241624 A 09.10.2008 . RU 2009482 C1 15.03.1994 . US 20030146159 A1 07.08.2003 . (86) Заявка PCT: 2 5 8 4 1 7 6 R U C 2 C 2 EP 2012/051512 (31.01.2012) (87) ...

Method for forming a concentrating device

The present invention relates to novel methods and apparatus for improving the sensitivity of capillary zone electrophoresis (CZE). The invention particularly concerns devices comprising a channel that contains an in-line sol-gel column to concentrate samples being subjected to capillary zone electrophoresis, and the use of such devices to enhance the sensitivity of capillary zone electrophoresis.

Methods and apparatus for improving the sensitivity of capillary zone electrophoresis

The present invention relates to novel methods and apparatus for improving the sensitivity of capillary zone electrophoresis (CZE). The invention particularly concerns devices comprising a channel that contains an in-line sol-gel column to concentrate samples being subjected to capillary zone electrophoresis, and the use of such devices to enhance the sensitivity of capillary zone electrophoresis.

Siloxane-immobilized particulate stationary phase for chromatographic separation and extraction

Device including a hydrophilic, non-ionic coating for size exclusion chromatography

The present disclosure is directed to a coating process for chromatographic surfaces. Embodiments of the present disclosure feature a two-step, vapor-liquid phase organosilane deposition method for creating a hydrophilic, non-ionic surface in a chromatographic system.

Microfluidic device and method for batch adsorption

The invention relates to a fluidic device (1) having a particle trap functionality for a particle (2) in a fluid, the fluidic device (1) comprising a first fluid flow channel (11), a second fluid flow channel (12), and a control channel (13), wherein the first fluid flow channel (11) is in fluid contact with the second fluid flow channel (12) and intersects the second fluid flow channel (12) at an intersection (14), wherein the control channel (13) at the location of the intersection (14) is arranged adjacent to the first fluid flow channel (11) and separated by a flexible membrane (17) from the intersection (14), wherein the flexible membrane (17) is configured as a valve (31), configured in operation at a first pressure in the control channel (13) in a first position not to block propagation of a particle (2) of a predetermined size in the first fluid flow channel (11), and at a second pressure in the control channel (13) in a second position to block with said flexible membrane (17) propagation of said particle (2) through the first fluid flow channel (11), while in this second position not fully blocking fluid flow through the first fluid flow channel (11) wherein the valve (31) is configured to provide in said second position a gradual increasing blockade of a cross sectional area (19) of the first fluid flow channel (11) in a direction parallel to a longitudinal axis of the first fluid flow channel (11).

Column manufacturing apparatus and manufacturing method

カラム内に圧力が均一な充填剤の堆積層が形成されたカラムの製造方法を提供すること。また、カラム内の圧力の個体差が極小化され、安定した試料の分解能を有するカラムの製造装置及び製造方法を提供すること。管体と、前記管体の一方の端部に配備されて、充填剤を溶媒に分散してなる充填剤スラリーを前記管体に充填する充填剤供給機構と、前記管体の他方の端部側に配備されて、充填中の前記充填剤スラリーの流量及び圧力を予め定められた流量及び圧力で保持する充填制御手段とを備えることを特徴とするカラムの製造装置。また、管体の一方の端部に備える充填剤供給機構から、充填剤を溶媒に分散してなる充填剤スラリーを前記管体に充填するカラムの製造方法であって、前記充填剤供給機構から前記管体に前記充填剤スラリーを充填しつつ、前記管体の他方の端部側に配備されている充填制御手段によって前記充填剤スラリーの流量及び圧力を予め定められた流量及び圧力で保持することを特徴とするカラムの製造方法。 To provide a method for producing a column in which a deposit layer of a filler having a uniform pressure is formed in the column. Also, the present invention provides a column manufacturing apparatus and a manufacturing method in which individual differences in pressure in the column are minimized and stable sample resolution is provided. A tubular body, a filler supply mechanism that is disposed at one end of the tubular body and fills the tubular body with a filler slurry obtained by dispersing a filler in a solvent, and the other end of the tubular body A column manufacturing apparatus, comprising: packing control means that is disposed on the side and holds the flow rate and pressure of the filler slurry during packing at a predetermined flow rate and pressure. Also, a method for producing a column in which a filler slurry prepared by dispersing a filler in a solvent is packed into the pipe body from a filler supply mechanism provided at one end of the pipe body, the method comprising: While filling the tube body with the filler slurry, the flow rate and pressure of the filler slurry are maintained at a predetermined flow rate and pressure by the filling control means arranged on the other end side of the tube body. A method for producing a column characterized by the above.

Chromatography tubing string

本发明获得一种当进行色谱法时，可将填充材料以最合适的压缩状态填充，进而防止包含试验体的流动相从管中漏出的管柱。管柱10主要包括：筒状的管100、管塞200、第一过滤器310、第二过滤器320、塞子400、及连接器500。管100主要包括：有底圆筒状的收纳部110、及与收纳部110的底部连接的圆筒状的底侧连接部130。收纳部110与底侧连接部130设置在同轴上。管塞200主要包括：设置在同轴上的圆筒形的外筒部210、及设置在外筒部210的内周侧的内筒部220。第一过滤器310及第二过滤器320是填充材料330无法穿过的程度的过滤器。塞子400主要包括塞子握持部401与塞子插入部402。连接器500主要包括连接器握持部501与连接器插入部502。

Gas chromatograph column and its production

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Chromatography device

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Filter holder in chromatography column

【課題】クロマトグラフィーカラムに関し、カラム内の流体分配に使用される粒子保持フィルターの安定した固定に関する。【解決手段】クロマトグラフィーカラムに使用されるフィルターホルダーであって、フィルターホルダーは、プレートの形態である、カラムのフィルターに面するフィルター締付け部品３５６と、フィルターホルダーとフィルター２０４との間でのカラムの吸着床スペース２０９からの粒状媒体の漏れを防ぐため、フィルター締付け部品３５６をフィルター２０４に対して締め付けるように適合された締付け手段３５４とを備える。フィルター締付け部品３５６は、フィルター締付け部品３５６を通してフィルター締付け部品３５６のフィルター２０４に面する面からフィルター締付け部品３５６の吸着床スペース２０９に面する面への流体接続を提供する、１つ以上のチャネル３３８を備える。【選択図】図２

Chromatography system

A chromatography system comprises a chromatography column 1 consisting of a column tube 7 having end flanges 19 and 27 at its lower and upper ends, supporting an upper end cap 3 carrying a piston rod 9 and a piston 11. The system further comprises a container 20 for the separation media used in the chromatography column, this container having a neck ring 24 of injection moulded construction with a mouth end of a bag 22 embedded therein.

PROCESS FOR FILLING A NARROW COLUMN WITH MICRO PARTICLES AND CORRESPONDING PRODUCT

L'INVENTION CONCERNE LES TECHNIQUES CHROMATOGRAPHIQUES. ON FABRIQUE UNE COLONNE CHROMATOGRAPHIQUE DE LA MANIERE SUIVANTE: ON CHOISIT UNE COLONNE FLEXIBLE 10 DE DIAMETRE INFERIEUR A 500MM; ON FORME DANS UN RESERVOIR 17 UNE BOUILLIE CONSTITUEE PAR UN SOLVANT MOBILE ET PAR DES PARTICULES 18 DE TAILLE SPECIFIEE; ON PLACE UNE RESTRICTION A L'EXTREMITE DE LA COLONNE POUR PERMETTRE L'ECOULEMENT DU SOLVANT MOBILE TOUT EN EMPECHANT LE PASSAGE DES PARTICULES; ON RELIE LE RESERVOIR A LA COLONNE ET ON INTRODUIT DANS LA COLONNE LA BOUILLIE SOUS PRESSION, EN PARTANT D'UNE PRESSION INITIALE QU'ON AUGMENTE ENSUITE JUSQU'A UNE PRESSION FINALE. APPLICATION A L'ANALYSE CHIMIQUE. THE INVENTION CONCERNS CHROMATOGRAPHIC TECHNIQUES. A CHROMATOGRAPHIC COLUMN IS MANUFACTURED IN THE FOLLOWING MANNER: A FLEXIBLE COLUMN 10 WITH A DIAMETER LESS THAN 500MM IS CHOSED; A SLURRY CONSTITUTED BY A MOBILE SOLVENT AND PARTICLES 18 OF A SPECIFIED SIZE IS FORMED IN A TANK 17; A RESTRICTION IS PLACED AT THE END OF THE COLUMN TO ALLOW THE FLOW OF THE MOBILE SOLVENT WHILE PREVENTING THE PASSAGE OF PARTICLES; THE RESERVOIR IS CONNECTED TO THE COLUMN AND THE SPRAY UNDER PRESSURE IS INTRODUCED INTO THE COLUMN, STARTING FROM AN INITIAL PRESSURE THAT IS THEN INCREASED UNTIL A FINAL PRESSURE. APPLICATION TO CHEMICAL ANALYSIS.

Patent FR2028220A1

Method and apparatus for collecting gas chromatographic fractions

METHOD FOR COATING A SOLID SURFACE CARRYING EXPOSED SILANOL GROUPS TO REDUCE THE PRESENCE OF THEIR ELECTROSTATIC CHARGES, AND APPLICATION TO PROTEIN SEPARATION

Procédé pour revêtir une surface solide, portant des groupes silanols exposés, pour diminuer la présence de leurs charges électro-statiques, et application à la séparation de protéines. On applique une couche de revêtement, stable à l'hydrolyse, en transformant les groupes silanols de la surface solide en des groupes halogénures de silicium, que l'on fait ensuite réagir avec un réactif organométallique comportant un fragment éthényle terminal pour obtenir des groupes Si-R (R conservant le fragment éthényl terminal) et en faisant ensuite reagir ces groupes éthényles avec un monomère organique, neutre, par polymérisation d'addition, pour former sur la surface à protéger une couche monomoléculaire de polymère non réticulé La couche de polymère, reliée directement, est stable dans une large gamme de pH. Application: traitement des parois intérieures de tubes capillaires en silice (fondue) pour des séparations par chromatographie et électrophorèse. Process for coating a solid surface, bearing exposed silanol groups, to decrease the presence of their electrostatic charges, and application to the separation of proteins. A coating layer, stable to hydrolysis, is applied by transforming the silanol groups of the solid surface into silicon halide groups, which is then reacted with an organometallic reagent comprising a terminal ethenyl moiety to obtain Si groups. -R (R retaining the terminal ethenyl moiety) and then reacting these ethenyl groups with an organic, neutral monomer, by addition polymerization, to form on the surface to be protected a monomolecular layer of uncrosslinked polymer The polymer layer, directly linked, is stable over a wide pH range. Application: treatment of the inner walls of capillary tubes in silica (fused) for separations by chromatography and electrophoresis.

Patent FR2020351A1

CUP BED CARTRIDGE

L'INVENTION CONCERNE LA FABRICATION DE CARTOUCHES A LIT TASSE POUR L'ANALYSE CHIMIQUE. UNE CARTOUCHE CORRESPONDANT A L'INVENTION COMPREND ESSENTIELLEMENT UN TUBE THERMORETRACTABLE 10 QUI CONTIENT UN LIT TASSE DE PARTICULES, ENTRE DEUX ELEMENTS DE RETENUE 12 ET 14 CONSTITUES PAR UNE MATIERE SOLIDE POREUSE. LA RETRACTION DU TUBE SOUS L'EFFET DE LA CHALEUR EXERCE UNE COMPRESSION TRI-AXIALE SUR LE LIT 18, CE QUI AMELIORE SES CARACTERISTIQUES. APPLICATION A LA CHROMATOGRAPHIE EN PHASE LIQUIDE. THE INVENTION RELATES TO THE MANUFACTURE OF CUP BED CARTRIDGES FOR CHEMICAL ANALYSIS. A CARTRIDGE CORRESPONDING TO THE INVENTION ESSENTIALLY CONSISTS OF A HEAT SHRINKABLE TUBE 10 WHICH CONTAINS A CUMPED BED OF PARTICLES, BETWEEN TWO RETAINERS 12 AND 14 CONSTITUTED BY A SOLID POROUS MATERIAL. THE RETRACTION OF THE TUBE UNDER THE EFFECT OF THE HEAT EXERCISES A TRI-AXIAL COMPRESSION ON THE BED 18, WHICH IMPROVES ITS CHARACTERISTICS. APPLICATION TO LIQUID PHASE CHROMATOGRAPHY.

Patent FR1570940A

Improvement to chromatography apparatuses

The subject of the present invention is a chromatography apparatus or chromatography column. This apparatus comprises a sliding body 5, which is displaced by the pressure exerted by a pneumatic or hydraulic pressurised fluid injected into an enclosure 9 formed between the face of the sliding body opposite that which is intended to be placed in contact with the packing and the end of the tube 2 which faces it. It relates to an improvement to chromatography apparatuses.

Patent FR2413933B1

Method for the analysis of liquids or solutions by liquid chromatography

Improvements to the filling of isotope separation columns by chromatography, and preparation process

LIQUID PHASE CHROMATOGRAPHY APPARATUS

PROCESS FOR FILLING CHROMATOGRAPHY COLUMNS

L'invention concerne les colonnes de chromatographie et leur procédé de garnissage. Le garnissage est réalisé par introduction de la matière dans la colonne en présence d'une première pression différentielle et sans vibration périodique régulière, puis par application d'une vibration périodique sensiblement régulière en présence d'une seconde pression différentielle supérieure à la première. Application au remplissage des colonnes de chromatographie. The invention relates to chromatography columns and their packing method. The packing is carried out by introducing the material into the column in the presence of a first differential pressure and without regular periodic vibration, then by applying a substantially regular periodic vibration in the presence of a second differential pressure greater than the first. Application to the filling of chromatography columns.

CHROMATOGRAPHY DEVICE AND METHOD FOR IMPLEMENTING THE SAME

DISPOSITIF DE CHROMATOGRAPHIE ET PROCEDE DE MISE EN OEUVRE. L'INVENTION EST RELATIVE A UN DISPOSITIF DE CHROMATOGRAPHIE PREPARATIVE EN PHASE LIQUIDE COMPRENANT UNE COLONNE SOUS FORME D'UN TUBE CYLINDRIQUE MUNI A SA PARTIE INFERIEURE D'UN COUVERCLE; UN DISPOSITIF DE REMPLISSAGE PR DESTINE A ETRE RELIE PAR UNE BUSE DE COMPRESSION ET COMPRENANT UN VERIN ACTIONNANT UN CORPS SOLIDE NON POREUX PERMETTANT DE COMPRIMER LA PHASE STATIONNAIRE REMPLISSANT LADITE COLONNE PAR L'INTERMEDIAIRE DE LA BUSE DE COMPRESSION; UNE TETE D'INJECTION DESTINEE A ETRE FIXEE SUR LA COLONNE APRES SON REMPLISSAGE C, LADITE TETE D'INJECTION COMPRENANT UNE PAROI INFERIEURE ET UN BOITIER D'INJECTION D'UN DIAMETRE INFERIEUR A LA TETE D'INJECTION MUNI DE PLUSIEURS ARRIVEES LATERALES DE SOLUTE, LADITE TETE D'INJECTION COMPORTANT SUR LA FACE SUPERIEURE UNE ARRIVEE CENTRALE DE SOLVANT ET DES MOYENS PERMETTANT AU SOLVANT DE CONTOURNER LE BOITIER D'INJECTION; UNE POMPE P RELIEE A LADITE TETE D'INJECTION COMPRENANT QUATRE PISTONS ACTIONNES PAR DES CAMES OU DES BIELLES DISPOSEES A 90 LES UNES PAR RAPPORT AUX AUTRES DONT LE SUPPORT N'EST PAS SOLIDAIRE DU BLOC; UN DISPOSITIF DE DETECTION CONSISTANT EN UN MONTAGE EN DOUBLE DETECTION D; UN COLLECTEUR DE FRACTIONS RELIE AU DISPOSITIF DE DETECTION COMPRENANT DEUX COMPARTIMENTS SUPERPOSES. CHROMATOGRAPHY DEVICE AND PROCESS FOR IMPLEMENTATION. THE INVENTION RELATES TO A PREPARATIVE LIQUID PHASE CHROMATOGRAPHY DEVICE INCLUDING A COLUMN IN THE FORM OF A CYLINDRICAL TUBE WITH ITS LOWER PART WITH A COVER; A PR FILLING DEVICE INTENDED TO BE CONNECTED BY A COMPRESSION NOZZLE AND INCLUDING A CYLINDER ACTIVATING A SOLID NON-POROUS BODY ALLOWING TO COMPRESS THE STATIONARY PHASE FILLING THE said COLUMN THROUGH THE COMPRESSION NOZZLE; AN INJECTION HEAD INTENDED TO BE FIXED ON THE COLUMN AFTER ITS FILLING C, THE AID INJECTION HEAD INCLUDING A BOTTOM WALL AND AN INJECTION BOX WITH A DIAMETER LOWER THAN THE INJECTION HEAD ...

IMPROVEMENT IN CHROMATOGRAPHY DEVICES

Patent FR2300598B1

Separation column for chromatographic analysis

Carrier or adsorbent for chromatography and process for their production