16-05-2013 дата публикации
Номер: US20130121530A1
The invention relates to a microscopy method for identifying target objects () having a predetermined optical property in material () to be analyzed. 132632. A microscopy method for identifying target objects () in material () to be analyzed , the target objects () having a predetermined optical property , wherein{'b': 36', '14', '4', '6, 'in a first step, an overview field of view () of a microscope optical system () is directed to an overview region of a sample carrier () containing the material () to be analyzed;'}{'b': 6', '16', '4', '48', '8, 'the material () to be analyzed is illuminated by an illumination unit (), which irradiates the sample carrier () from outside a field of view tube (), and is recorded by a camera ();'}{'b': 6', '32', '6, 'the material () to be analyzed is optically analyzed for the optical property such that even a single target object () having the predetermined optical property is identified as such in the material () to be analyzed;'}{'b': 52', '14', '32', '32, 'in a subsequent second step, a target field of view () of the microscope optical system () is aligned with a target region around the target object () using the known position of the target object (); and'}{'b': '32', 'the identified target object () is analyzed in a manner differentiated for various additional optical properties.'}25236. The microscopy method according to claim 1 , characterized in that the target field of view () has a magnification by at least a factor of 3 over the overview field of view ().33232. The microscopy method according to claim 1 , characterized in that the identification of the target object () in the first step is carried out with a first optical method and the differentiation of the target object () in the second step is carried out with a second optical method which is different from the first.461832. The microscopy method according to claim 1 , characterized in that claim 1 , in the second step claim 1 , the material () to be analyzed is ...
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