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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 2453. Отображено 100.
15-03-2012 дата публикации

Apparatus and method for elemental analysis of particles by mass spectrometry

Номер: US20120061561A1
Автор: Alexei Antonov, Dmitry Roman Bandura
Принадлежит: DVS Sciences Inc

An apparatus for elemental analysis of particles such as single cells or single beads by mass spectrometry is described. The apparatus includes means for particle introduction; means to vaporize, atomize and ionize elements associated with a particle; means to separate the ions according to their mass-to-charge ratio; means to detect the separated ions, means to digitize the output of the means to detect the ions; means to transfer and/or to process and/or record the data output of the means to digitize, having means to detect the presence of a particle in a mass spectrometer; and means to synchronize one of the means for ion detection, data digitization, transfer, processing and recording with the means to detect the presence of a particle. Methods and computer readable code implementing aspects of the apparatus, and for reducing the rates of data generation, digitization, transfer, processing and recording are also described.

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Номер записи: 1
17-05-2012 дата публикации

Ion generation using wetted porous material

Номер: US20120119079A1
Автор: HE Wang, Jiangjiang Liu, Nicholas E. Manicke, Qian Yang, Robert Graham Cooks, Zheng Ouyang
Принадлежит: PURDUE RESEARCH FOUNDATION

The invention generally relates to systems and methods for mass spectrometry analysis of samples. In certain embodiments, the invention provides a mass spectrometry probe including at least one porous material connected to a high voltage source, in which the porous material is discrete from a flow of solvent.

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Номер записи: 2
04-10-2012 дата публикации

Mass spectrometer

Номер: US20120248305A1
Автор: Hidetoshi Morokuma, Hiroyuki Noda, Koji Ishiguro, Mitsuru Onuma, Nami IBI, Shigeo Otsuki, Yoko Sato
Принадлежит: Hitachi High Technologies Corp

In the spectrometer, heavy loads are arranged centrally inside a case having a height smaller than a width, and having a depth smaller than the height. The heavy loads include a vacuum chamber, a vacuum pump which evacuates the vacuum chamber, a sample introduction unit which introduces a sample to be measured and evaporates the sample, an ionization unit which ionizes the evaporated sample and provides it to the vacuum chamber, and an ion detection unit which is connected to the vacuum chamber. Circuit board storage units which store a plurality of circuit boards with a predetermined space therebetween are formed on both sides along a width direction of the case.

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Номер записи: 3
13-12-2012 дата публикации

Enclosed desorption electrospray ionization probes and method of use thereof

Номер: US20120312979A1
Автор: Chien-Hsun Chen, Livia Schiavinato Eberlin, Robert Graham Cooks, Zheng Ouyang, Ziqing Lin
Принадлежит: PURDUE RESEARCH FOUNDATION

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

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Номер записи: 4
25-04-2013 дата публикации

IN-SITU CONDITIONING IN MASS SPECTROMETER SYSTEMS

Номер: US20130099113A1
Автор: FREED MICHAEL K., PREST HARRY F., QUIMBY BRUCE D., SZELEWSKI MICHAEL J.
Принадлежит: AGILENT TECHNOLOGIES, INC.

In a mass spectrometer or gas chromatograph/mass spectrometer system, a conditioning gas such as, for example, hydrogen is added to condition or clean one or more components or regions of the mass spectrometer such as the ion source. The conditioning gas may be added upstream of the mass spectrometer such as, for example, into a sample inlet or a chromatographic column, or may be added directly into the mass spectrometer. The conditioning gas may be added off-line, when the mass spectrometer is not analyzing a sample, or on-line during sample analysis. When added on-line, the conditioning gas may be mixed with a carrier gas such as, for example, helium. In another embodiment, the conditioning gas also serves as the carrier gas through the column; another gas such as, for example, helium may be added to the carrier gas stream. 120.-. (canceled)21. A method for operating a mass spectrometer (MS) system , the method comprising:introducing a sample and a carrier gas into an ionization chamber of the MS system; andflowing a conditioning gas into the MS system, the conditioning gas comprising hydrogen gas, wherein the conditioning gas in the MS system does not substantially change the mass spectral characteristics of analytes of the sample, and the carrier gas does not comprise hydrogen gas.22. The method of claim 21 , wherein the MS system comprises a collision cell.23. The method of claim 22 , wherein the conditioning gas is flowed directly into the collision cell.24. The method of claim 22 , wherein the conditioning gas is flowed into the MS system upstream from the collision cell.25. The method of claim 22 , wherein the conditioning gas is flowed into the MS system downstream from the collision cell.26. The method of claim 21 , wherein the conditioning gas is flowed directly into an ion detector of the MS system.27. The method of claim 21 , wherein the carrier gas comprises helium.28. The method of claim 21 , wherein the carrier gas is helium and the conditioning gas ...

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Номер записи: 5
25-04-2013 дата публикации

Early detection of thiamine deficiency

Номер: US20130102624A1
Автор: John V. Schloss
Принадлежит: Individual

A method is provided for determining the thiamine status of a human or animal based on the relative levels of thiamine and its metabolites. Certain embodiments of the present invention also provide methods for determining the effectiveness of a thiamine deficiency treatment.

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Номер записи: 6
02-05-2013 дата публикации

DISCONTINUOUS ATMOSPHERIC PRESSURE INTERFACE

Номер: US20130105683A1
Автор: Cooks Robert Graham, Gao Liang, Ouyang Zheng
Принадлежит:

A method of interfacing atmospheric pressure ion sources, including electrospray and desorption electrospray ionization sources, to mass spectrometers, for example miniature mass spectrometers, in which the ionized sample is discontinuously introduced into the mass spectrometer. Discontinuous introduction improves the match between the pumping capacity of the instrument and the volume of atmospheric pressure gas that contains the ionized sample. The reduced duty cycle of sample introduction is offset by operation of the mass spectrometer under higher performance conditions and by ion accumulation at atmospheric pressure. 127-. (canceled)28. An analysis system , the system comprising:an ionizing source that generates a continuous flow of gas phase ions;a discontinuous atmospheric pressure interface that receives the gas phase ions from the ionizing source; anda mass analyzer of a miniature mass spectrometer that discontinuously receives ions from the discontinuous atmospheric pressure interface, the system being configured such that the mass analyzer is periodically prevented from receiving any ions.29. The system according to claim 28 , wherein the discontinuous atmospheric pressure interface comprises a valve.30. The system according to claim 29 , further comprising a computer operably connected to the system claim 29 , wherein the computer contains a processor configured to execute a computer readable program claim 29 , the program controlling the position of the valve.31. The system according to claim 29 , wherein the valve operates to control entry of ions in a synchronized manner with respect to operation of the mass analyzer.32. The system according to claim 28 , wherein the ionizing source operates by a technique selected from the group consisting of: electrospray ionization claim 28 , nano-electrospray ionization claim 28 , atmospheric pressure matrix-assisted laser desorption ionization claim 28 , atmospheric pressure chemical ionization claim 28 , ...

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Номер записи: 7
09-05-2013 дата публикации

Method of Avoiding Space Charge Saturation Effects In An Ion Trap

Номер: US20130112865A1
Автор: Jason Lee Wildgoose, Martin Raymond Green
Принадлежит: Micromass UK Ltd

A mass spectrometer includes a first ion trap arranged upstream of an analytical second ion trap. The charge capacity of the first ion trap is set at a value such that if all the ions stored within the first ion trap up to the charge capacity limit of the first ion trap are then transferred to the second ion trap, then the analytical performance of the second ion trap is not substantially degraded due to space charge effects.

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Номер записи: 8
09-05-2013 дата публикации

ION GENERATION USING WETTED POROUS MATERIAL

Номер: US20130112866A1
Автор: Cooks Robert Graham, Liu Jiangjiang, Manicke Nicholas E., Ouyang Zheng, Wang He, Yang Qian
Принадлежит:

The invention generally relates to systems and methods for mass spectrometry analysis of samples. In certain embodiments, the invention provides a mass spectrometry probe including at least one porous material connected to a high voltage source, in which the porous material is discrete from a flow of solvent. 147-. (canceled)48. A method for analyzing a food or beverage sample , the method comprising:introducing a food or beverage sample to at least one porous material;applying a high voltage to the porous material to generate ions of an analyte in the sample that are expelled from the porous material; andanalyzing the expelled ions, thereby analyzing the food or beverage sample.49. The method according to claim 48 , further comprising applying a solvent to the porous material.50. The method according to claim 49 , wherein the porous material is kept separate from a flow of solvent.51. The method according to claim 49 , wherein the solvent assists transport of the sample through the porous material.52. The method according to claim 49 , wherein the solvent contains an internal standard.53. The method according to claim 49 , wherein the solvent allows for differential retention of sample components with different chemical properties.54. The method according to claim 49 , wherein the solvent minimizes salt and matrix effects.55. The method according to claim 49 , wherein the solvent allows for on-line chemical derivatization of selected analytes.56. The method according to claim 48 , wherein the food sample comprises fruit.57. The method according to claim 48 , wherein the food sample comprises coffee.58. The method according to claim 48 , wherein the beverage sample comprises soda.59. The method according to claim 48 , wherein the porous material is paper or PVDF membrane.60. The method according to claim 59 , wherein the paper is filter paper.61. The method according to claim 60 , wherein the filter paper is shaped to have a side that terminates in a point.62. The ...

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Номер записи: 9
09-05-2013 дата публикации

ION GENERATION USING WETTED POROUS MATERIAL

Номер: US20130112867A1
Автор: Cooks Robert Graham, Liu Jiangjiang, Manicke Nicholas E., Ouyang Zheng, Wang He, Yang Qian
Принадлежит:

The invention generally relates to systems and methods for mass spectrometry analysis of samples. In certain embodiments, the invention provides a mass spectrometry probe including at least one porous material connected to a high voltage source, in which the porous material is discrete from a flow of solvent. 147-. (canceled)48. A method for analyzing an agricultural sample , the method comprising:introducing an agricultural sample to at least one porous material;applying a high voltage to the porous material to generate ions of an analyte in the sample that are expelled from the porous material; andanalyzing the expelled ions, thereby analyzing the agricultural sample.49. The method according to claim 48 , further comprising applying a solvent to the porous material.50. The method according to claim 49 , wherein the porous material is kept separate from a flow of solvent.51. The method according to claim 49 , wherein the solvent assists transport of the sample through the porous material.52. The method according to claim 49 , wherein the solvent contains an internal standard.53. The method according to claim 49 , wherein the solvent allows for differential retention of sample components with different chemical properties.54. The method according to claim 49 , wherein the solvent minimizes salt and matrix effects.55. The method according to claim 49 , wherein the solvent allows for on-line chemical derivatization of selected analytes.56. The method according to claim 48 , wherein the agricultural sample comprises plant tissue.57. The method according to claim 48 , wherein the agricultural sample comprises fruit.58. The method according to claim 48 , wherein the porous material is paper or PVDF membrane.59. The method according to claim 58 , wherein the paper is filter paper.60. The method according to claim 59 , wherein the filter paper is shaped to have a side that terminates in a point.61. The method according to claim 60 , wherein the filter paper has a ...

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Номер записи: 10
23-05-2013 дата публикации

Measurement Of 25-Hydroxyvitamin D3 And C3 EP1-25-Hydroxyvitamin D3

Номер: US20130126721A1
Автор: Carlton Lisa J., Molloy Billy J.
Принадлежит: WATERS TECHNOLOGIES CORPORATION

The invention describes a method of quantification of an analyte selected from the group consisting of 25-hydroxyvitamin D3 and C3-epi-25-hydroxyvitamin D3 in a specimen containing the analyte; comprising the steps of subjecting the specimen to UPLC reverse-phase separation; and; detecting the protonated precursor pseudo-molecular ion of the analyte using a mass spectrometry technique to determine the amount of the analyte. 1. A method of quantification of an analyte selected from the group consisting of 25-hydroxyvitamin D3 and C3-epi-25-hydroxyvitamin D3 in a specimen containing the analyte;comprising the steps of subjecting the specimen to UPLC reverse-phase separation; and;detecting the protonated precursor pseudo-molecular ion of the analyte using a mass spectrometry technique to determine the amount of the analyte.2. A method as claimed in claim 1 , wherein the mass spectrometry technique comprises MRM detection of the protonated precursor pseudo-molecular ion.3. A method as claimed in or claim 1 , wherein the separation includes solid phase extraction.4. A method as claimed in any preceding claim claim 1 , wherein the C3-epi-25-hydroxy vitamin D3 is separated from 25-hydroxy vitamin D3 by ion mobility.5. A method as claimed in any preceding claim claim 1 , wherein the sample is a biological sample.6. A method as claimed in any preceding claim claim 1 , wherein deuterated 25OHD2 is used as an internal standard.7. A method as claimed in any preceding claim wherein the UPLC mobile phases comprise water and methanol.8. A method as claimed in any preceding claim wherein a solvent gradient curve is employed.9. A method as claimed in claim 8 , wherein a Waters (RTM) gradient curve 10 is employed. This invention relates to the separation and quantification of the C3-epimer of 25-hydroxyvitamin D3 (25OHD3) in serum or other specimens.Vitamin D is a generic designation for a group of fat-soluble structurally similar sterols. Vitamin D compounds are derived from dietary ...

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Номер записи: 11
23-05-2013 дата публикации

SYSTEMS AND METHODS FOR TRANSFER OF IONS FOR ANALYSIS

Номер: US20130126723A1
Автор: Charipar Nicholas Alan, Cooks Robert Graham, Garimella Sandilya Venkata, Harper Jason David, Ouyang Zheng
Принадлежит:

The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device. 1. A system for analyzing a sample , the system comprising:an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure;an ion analysis device; andan ion transfer member operably coupled to a gas flow generating device, wherein the gas flow generating device produces a laminar gas flow that focuses and transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.2. The system according to claim 1 , wherein the ions are transferred over a distance of at least about five centimeters.3. The system according to claim 1 , wherein the ions are sampled over a area at least 4 centimeters by 3 centimeters.4. The system according to claim 1 , wherein the gas flow generating device is a pump.5. The system according to claim 1 , wherein the gas flow generating device is a gas jet of the ionizing source.6. The system according to claim 1 , further comprising an electric focusing lens device operably coupled to the ion transfer member to facilitate transfer of ions to the inlet of the ion analysis device.7. The system according to claim 6 , wherein the electric focusing element further focuses the ions at the center of the transfer member during the transfer.812-. (canceled)12. The system according to claim 1 , wherein the ion transfer member is a tube.13. The system according to claim 12 , wherein the tube is composed of ...

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Номер записи: 12
23-05-2013 дата публикации

Electrostatic Trap

Номер: US20130126724A1
Автор: Balschun Wilko, Denisov Eduard V., HORNING Stevan R., Jung Gerhard, MAKAROV Alexander A.
Принадлежит:

An electrostatic trap such as an orbitrap is disclosed, with an electrode structure. An electrostatic trapping field of the form U′ (r, Φ, z) is generated to trap ions within the trap so that they undergo isochronous oscillations. The trapping field U′(r, Φ, z) is the result of a perturbation W to an ideal field U(r, Φ, z) which, for example, is hypologarithmic in the case of an orbitrap. The perturbation W may be introduced in various ways, such as by distorting the geometry of the trap so that it no longer follows an equipotential of the ideal field U(r, Φ, z), or by adding a distortion field (either electric or magnetic). The magnitude of the perturbation is such that at least some of the trapped ions have an absolute phase spread of more than zero but less than 2 n radians over an ion detection period T. 1. A method of trapping ions in an electrostatic trap having at least one trapping electrode , comprising:applying a substantially electrostatic potential to the at least one electrode to generate an electrostatic field that causes an ion to undergo oscillatory movement along a first axis;wherein a period of the oscillatory movement is dependent upon an amplitude of the oscillatory movement.2. The method of claim 1 , wherein the period increases with an increase in the amplitude.3. The method of claim 1 , wherein the at least one trapping electrode comprises first and second electrodes defining a trapping region therebetween claim 1 , the first and second electrodes being elongate along the first axis.4. The method of claim 1 , wherein the electrostatic field approximates a hyper-logarithmic field.5. The method of claim 1 , wherein the period varies according to the mass-to-charge ratio of the ion.6. An electrostatic trap claim 1 , comprising:at least first and second electrodes defining therebetween a trapping volume;wherein the first and second electrodes are arranged to generate a trapping field within the trapping volume when a trapping potential is applied ...

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Номер записи: 13
30-05-2013 дата публикации

POST-IONIZATION OF NEUTRALS FOR ION MOBILITY oTOFMS IDENTIFICATION OF MOLECULES AND ELEMENTS DESORBED FROM SURFACES

Номер: US20130134305A1
Автор: Egan Thomas F., Lewis Ernest K., Schultz J. Albert, Waters Kelley L.
Принадлежит: IONWERKS, INC.

The present invention relates to a method and apparatus for ionizing a neutral MALDI desorption plume, and in particular, for efficiently measuring the ionized MALDI desorption plume when post-ionization techniques are combined with a medium pressure MALDI-IM-oTOFMS instrument. Additionally, the present disclosure provides a method and apparatus that simultaneously separates tissue-sample MALDI ions by IM-oTOFMS according to their chemical family. After separation, the MALDI ions are directly compared to the ions created by post-ionizing the co-desorbed neutral molecules with a second laser wherein the second laser is delayed by a few hundred microseconds. The present disclosure further provides novel approaches that enhance the analysis of ions, including the use of giant fullerene internal standards to enhance mass accuracy, and ultraviolet (UV) declustering lasers to generate intact peptides and proteins, either of which may be followed by VUV post-ionization which generates identifiable structural fragments. 1. An apparatus comprising:an ion source for repetitively or continuously generating ions and neutrals;a post-ionization device fluidly coupled to said ion source to post-ionize or fragment at least a fraction of said ions and neutrals;an ion mobility cell capable of receiving the directly desorbed ions and the post-ionized ions;an ion extractor, fluidly coupled to said ion-mobility device capable of extracting said ions;a mass spectrometer fluidly coupled to and accepting said ions and fragment ions from said ion extractor,a position sensitive ion detector fluidly coupled to said time-of-flight mass spectrometer to detect said ions and fragment ions;a timing controller in electronic communication with said ion source and said ion extractor said timing controller tracking and controlling the time of activation of said ion source and controlling the activation of the post-ionization device and activation of said ion extractor according to a predetermined ...

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Номер записи: 14
06-06-2013 дата публикации

GENE DETECTING METHODS WITHOUT USING PCR

Номер: US20130140451A1
Автор: Huang Lequn
Принадлежит:

Gene detecting methods without using PCR are disclosed. The methods comprise forming sandwich complexes by target genes with nano-probes and capture probes, wherein nano-probes are modified with recognition molecules and magnetic microparticles modified with capture molecules; then separating the sandwich complexes; releasing the nano-probes; and detecting molecular ion peaks of encoding molecules on the surface of nano-probes by mass spectrometric detection directly, characterized in that the proportions of recognition molecules and encoding molecules on the nano-probes are 300-2000:1. 1. A gene detecting method , comprise forming sandwich complexes by target genes with nano-probes and capture probes , wherein nano-probes are modified with recognition molecules and magnetic microparticles modified with capture molecules; then separating the sandwich complexes; releasing the nano-probes; and detecting molecular ion peaks of encoding molecules on the surface of nano-probes by mass spectrometric detection directly , characterized in that the proportions of recognition molecules and encoding molecules on the nano-probes are 300-2000:1 , and 1300-2000:1 are preferred.2. The gene detecting method as claimed in claim 1 , characterized in that the salt concentrations of the hybridization reaction system in which formed the sandwich complexes are 0.2˜1.0M claim 1 , and 0.5˜0.7M are preferred.3. The gene detecting method as claimed in claim 1 , characterized in that the mass spectrometry is matrix-assisted laser desorption ionization time of flight mass spectrometry or electrospray ionization mass spectrometry claim 1 , and matrix-assisted laser desorption ionization time of flight mass spectrometry is preferred.4. The gene detecting method as claimed in claim 3 , characterized in that the matrix used in the matrix-assisted laser desorption ionization time of flight mass spectrometry can be any kind of α-cyano-4-hydroxy cinnamic acid claim 3 , 3 claim 3 ,5-diethoxy.-4- ...

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Номер записи: 15
06-06-2013 дата публикации

VOLATILE ORGANIC COMPOUNDS FOR DETECTING CELL DYSPLASIA AND GENETIC ALTERATIONS ASSOCIATED WITH LUNG CANCER

Номер: US20130143247A1
Автор: Haick Hossam, Peled Nir
Принадлежит: TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LTD.

The present invention provides methods of identifying a genetic abnormality such as mutation in EGFR or KRAS or ALK which is associated with the management of lung cancer or diagnosing, prognosing or monitoring the treatment of pre-cancerous conditions of the lung, such as bronchial dysplasia or atypical alveolar hyperplasia (AAH), through the detection of at least one volatile organic compound indicative of these states. 1. A method of identifying a genetic alteration selected from a mutation in EGFR , a mutation in KRAS , an ALK-ELM4 translocation and CMET amplification , wherein the genetic alteration is associated with lung cancer , the method comprising the steps of:a) obtaining a sample from a test subject;b) determining the level of at least one volatile organic compound in the test sample; andc) comparing the level of the at least one volatile organic compound from the test sample with the level of said at least one volatile organic compound in a negative control sample, whereby a significantly different level of said at least one volatile organic compound in the test sample as compared to the level of said compound in the negative control sample is indicative of the presence of said genetic alteration.2. The method according to claim 1 , wherein the at least one volatile organic compound is selected from the group consisting of 4-methyl-1-heptanol claim 1 , acetic acid octyl ester claim 1 , decane claim 1 , 3-methyl-decane claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , and tetradecene; or selected from the group consisting of 4-methyl-1-heptanol claim 1 , 6-methyl-1-heptanol claim 1 , 2-ethyl-1-hexanol claim 1 , acetic acid octyl ester claim 1 , benzaldehyde claim 1 , decance claim 1 , 3-methyl-dodecance claim 1 , tetrahydrofuran claim 1 , isopropyl myristate claim 1 , octanal claim 1 , pentadecanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-pentanenitrile claim 1 , 2 claim 1 ,2 claim 1 ,4-trimethyl-3-carboxyisopropyl-isobutyl ester ...

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Номер записи: 16
13-06-2013 дата публикации

SYSTEMS AND METHODS FOR SAMPLE ANALYSIS

Номер: US20130146759A1
Автор: Cooks Robert Graham, Hou Keyong, Ouyang Zheng
Принадлежит:

The invention generally relates to improved sensitivity and flexibility for mass spectrometers with limited pumping capacity, particularly mass spectrometers that are coupled with a Discontinuous Atmospheric Pressure Interface (DAPI). 1. A method for increasing the sensitivity of a mass spectrometer equipped with a discontinuous atmospheric pressure interface , the method comprising: increasing vacuum volume of the mass spectrometer equipped with the discontinuous atmospheric pressure interface.2. The method according to claim , wherein the larger volume is achieved by using an elongated tube.3. The method according to claim 2 , wherein the tube is flexible.4. The method according to claim 3 , wherein the configuration is used to construct a sampling wand.5. The method of according to claim 1 , further comprising claim 1 , analyzing a sample.6. The method according to claim 5 , wherein analyzing comprises:ionizing a sample to generate ions of an analyte in the sample;discontinuously transferring the ions into the mass spectrometer; andgenerating a mass spectrum of analytes in the sample.7. The method according to claim 6 , wherein the ionizing is by a technique selected from the group consisting of: electrospray ionization claim 6 , nano-electrospray ionization claim 6 , atmospheric pressure matrix-assisted laser desorption ionization claim 6 , atmospheric pressure chemical ionization claim 6 , desorption electrospray ionization claim 6 , atmospheric pressure dielectric barrier discharge ionization claim 6 , atmospheric pressure low temperature plasma desorption ionization claim 6 , and electrospray-assisted laser desorption ionization.8. The method according to claim 1 , wherein the mass spectrometer is a benchtop or a handheld mass spectrometer.9. The method according to claim 1 , wherein the mass spectrometer comprises a mass analyzer.10. The method according to claim 9 , wherein the mass analyzer is selected from the group consisting of: a quadrupole ion trap ...

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Номер записи: 17
27-06-2013 дата публикации

MASS SPECTROMETER AND MASS SPECTROMETRY

Номер: US20130161507A1
Автор: Hashimoto Yuichiro, Morokuma Hidetoshi, NISHIMURA Kazushige, Sugiyama Masuyuki, Yamada Masuyoshi
Принадлежит: HITACHI HIGH-TECHNOLOGIES CORPORATION

A mass spectrometer featured in including an ion source including a first electrode, a second electrode, and a dielectric unit having a sample introducing unit and a sample discharging unit and provided between the first electrode and the second electrode, a power source of ionizing a sample by a discharge generated between the first electrode and the second electrode by applying an alternating current voltage to either one of the first electrode and the second electrode, a mass spectrometry unit of analyzing an ion discharged from the sample discharging unit, and a light irradiating unit of irradiating an area of generating the discharge with light. 1. A mass spectrometer comprising:an ion source that configures a first electrode, a second electrode, and a dielectric unit having a sample introducing unit and a sample discharging unit and provided between the first electrode and the second electrode;a power source that applies an alternating current voltage to either one of the first electrode and the second electrode and ionizes the sample by a discharge generated between the first electrode and the second electrode;amass spectrometry unit that analyzes an ion discharged from the sample discharging unit; anda light irradiating unit that irradiates an area of generating the discharge with light.2. The mass spectrometer according to claim 1 , further comprising:an irradiation controlling unit that controls an illuminance of the light irradiating unit,wherein the illumination controlling unit lowers the illuminance of the light irradiating unit when the mass spectrometer analyzes the ion.3. The mass spectrometer according to claim 2 , wherein the irradiation controlling unit switches off the light irradiating unit when the mass spectrometry unit analyzes the ion.4. The mass spectrometer according to claim 2 , wherein the irradiation controlling unit switches on the light irradiating unit during a portion or a total of a time period of applying the alternating current ...

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Номер записи: 18
25-07-2013 дата публикации

ANALYSIS METHOD, ADHESIVE TAPE, AND PEN

Номер: US20130187034A1
Автор: Nakatani Yoshimasa, Noritake Yuka, Shimada Haruo
Принадлежит: SHISEIDO COMPANY, LTD.

There is provided an analysis method including the steps of forming a layer including a calibration reagent, that can generate ions by using a DART ion source apparatus, in a predetermined area of a sample, and performing mass spectrometry on the ions generated from an area of the sample including the layer by using DART or DESI while moving the sample having the layer formed therein. 1. An analysis method by comprising the steps of:forming a layer including a calibration reagent, that can generate ions by using a DART ion source apparatus, in a predetermined area of a sample; andperforming mass spectrometry on the ions generated from an area of the sample including the layer by using DART or DESI while moving the sample having the layer formed therein.2. The analysis method as claimed in claim 1 , wherein the layer including the calibration agent is formed by adhering an adhesive tape including the calibration reagent.3. The analysis method as claimed in claim 1 , wherein the layer including the calibration agent is formed by using a pen filled with an ink including the calibration reagent.4. The analysis method as claimed in claim 1 , wherein the calibration reagent includes one or both of polyethylene glycol having a mass spectrum including equally spaced peaks (PEG 60-PEG 2000) or a fatty acid having a carbon number of 4-36.5. An adhesive tape comprising:a calibration reagent that can generate ions by using a DART ion source apparatus.6. The adhesive tape as claimed in claim 5 , wherein the calibration reagent includes one or both of polyethylene glycol having a mass spectrum including equally spaced peaks (PEG 60-PEG 2000) or a fatty acid having a carbon number of 4-36.7. A pen comprising:an ink filled in the pen and including a calibration reagent that can generate ions by using a DART ion source apparatus.8. The pen as claimed in claim 7 , wherein the calibration reagent includes one or both of polyethylene glycol having a mass spectrum including equally ...

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Номер записи: 19
25-07-2013 дата публикации

PARALLEL ION MASS AND ION MOBILITY ANALYSIS

Номер: US20130187037A1
Автор: JR. Anthony Joseph, Krueger Clinton Alawn, Midey, Osgood Mark A., Wu Ching
Принадлежит: Excellims Corporation

The present invention relates to a parallel IMS and MS measurement method where a sample flow is split and delivered to an IMS and a MS in parallel. A parallel acquisition MS/IMS method is used to supplement LC-MS and or MS data by using a synchronized MS/IMS acquisition. 1. A method for identifying components of a sample in mass spectral data comprising:a. splitting a sample flow into a mass analyzer and an ion mobility based separator for independent parallel ion mass and ion mobility analysis;b. acquiring mass spectral data of the sample using the mass analyzer while synchronously acquiring ion mobility data using the ion mobility based separator;c. performing a normalization between corresponding peaks of the mass spectral data and that of the peaks in the ion mobility data; andd. displaying which ion mobility peaks correspond to the mass spectra peaks.2. The method in claim 1 , wherein the normalization is accomplished by using a timing index and/or a calibrant.3. The method in claim 2 , wherein the timing index is created based on the timing of each flow component's arrival at one or more analytical instrument.4. The method in claim 3 , wherein the analytical instrument can be claim 3 , but not limited to: IMS claim 3 , MS claim 3 , conductivity detector claim 3 , UV detector claim 3 , diode-array or a detector.5. The method in claim 2 , further comprises a LC separation prior to splitting the sample flow into the mass analyzer and the ion mobility based separator.6. The method in claim 2 , wherein the timing index is created based on the components of the sample's chromatography properties whereby the resolution obtained using the LC separation is used as a reference to determine the timing index resolution.7. The method in claim 2 , wherein the calibrant is added to the sample flow or as one of the components of the sample.8. The method in claim 1 , wherein the sample flow comprises isomers.9. The method in claim 8 , wherein the isomers are constitutional ...

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Номер записи: 20
15-08-2013 дата публикации

Nanomanipulation Coupled Nanospray Mass Spectrometry (NMS)

Номер: US20130206976A1
Автор: Guido Fridolin Verbeck, IV
Принадлежит: University of North Texas System

A coupled nanomanipulation and nanospray mass spectrometry (NMS) system for single cell, single organelle, and ultra-trace molecular analysis is disclosed herein. The system primarily comprises a bio-workstation coupled to a NMS. The bio-workstation primarily comprises of a nanomanipulator stage with a plurality of nano-positioners attached to a cabinet with a piezo voltage source and a pressure injector. The present invention further describes a fingerprint lift method that when coupled with the system disclosed herein can be used for retrieval and analysis of trace amounts of drug and explosive residues. The system described herein has been used in the areas of trace and document analysis within the forensic field, trace fiber analysis, and electrostatic lifts for illicit drugs, as well as document and painting analysis.

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Номер записи: 21
29-08-2013 дата публикации

METHOD AND APPARATUS FOR IMPROVING THE THROUGHPUT OF A CHARGED PARTICLE ANALYSIS SYSTEM

Номер: US20130221216A1
Автор: Giannakopulos Anastassios, Makarov Alexander
Принадлежит:

A method of increasing ion throughput within an accumulator, an energy lift and a pulsed ion extractor, operated in that order upon a batch of ions, comprising the steps of: firstly loading a batch of ions into the accumulator; secondly changing the electrical potential of the energy lift to raise the energy of the batch of ions contained therein; and thirdly ejecting the batch of ions from the pulsed ion extractor; and wherein: the energy lift is a separate device from the accumulator and the pulsed ion extractor, and whilst changing the electrical potential in the second step a fresh batch of ions is loaded into the accumulator and/or a previous batch of ions is prepared for ejection in the pulsed ion extractor; or the energy lift is incorporated into the pulsed ion extractor and whilst changing the electrical potential in the second step a fresh batch of ions is loaded into the accumulator; or the energy lift is incorporated into the accumulator and whilst changing the electrical potential in the second step a previous batch of ions is prepared for ejection in the pulsed ion extractor. A charged particle analyzer system is also provided. 1. A method of increasing ion throughput within an accumulator , an energy lift and a pulsed ion source , operated in that order upon a batch of ions , comprising the steps of:(1) loading a batch of ions into the accumulator;(2) changing the electrical potential of the energy lift to raise the energy of the batch of ions contained therein; and wherein one of the following conditions is satisfied:', '(i) the energy lift is a separate device from the accumulator and the pulsed ion source, and concurrently with changing the electrical potential in step (2): a fresh batch of ions is loaded into the accumulator and/or a previous batch of ions is prepared for ejection in the pulsed ion source; or', '(ii) the energy lift is incorporated into the pulsed ion source and concurrently with changing the electrical potential in step (2) a ...

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Номер записи: 22
05-09-2013 дата публикации

FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETER AND METHOD FOR CONCENTRATING IONS FOR FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY

Номер: US20130228681A1
Автор: Choi Myoung Choul, Kim Hyun Sik, Kim Seung Yong, Park A Leum, Yoo Jong Shin
Принадлежит: KOREA BASIC SCIENCE INSTITUTE

A Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) includes: an ionization source generating ions; a deceleration lens, on which the ions generated by the ionization source and spatially dispersed are incident, selectively decelerating the incident ions so as to decrease the distance between the ions; and an ion cyclotron resonance cell on which the ions passing through the deceleration lens are incident. By preventing dispersing of ions due to mass difference and converging the ions using the deceleration lens, the mass range that can be measured at one time can be extended. Also, measurement sensitivity can be improved since the ions are effectively introduced to the ICR cell. 1. A Fourier transform ion cyclotron resonance mass spectrometer comprising:an ionization source generating ions;a deceleration lens, on which the ions generated by the ionization source and spatially dispersed are incident, selectively decelerating the incident ions so as to decrease the distance between the ions; andan ion cyclotron resonance cell on which the ions passing through the deceleration lens are incident.2. The Fourier transform ion cyclotron resonance mass spectrometer according to claim 1 , wherein the deceleration lens comprises a plurality of electrodes which are arranged along a moving direction of the ions and to configured to be applied with an electric potential claim 1 , and wherein each of the plurality of electrodes comprises a hole configured to allow passage of the ions.3. The Fourier transform ion cyclotron resonance mass spectrometer according to claim 2 , wherein claim 2 , an electric potential is applied to the plurality of electrodes for a predetermined time period while the ions pass through the hole of the plurality of electrodes.4. The Fourier transform ion cyclotron resonance mass spectrometer according to claim 3 , wherein the electric potential of the plurality of electrodes forms an electric potential gradient along the moving ...

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Номер записи: 23
03-10-2013 дата публикации

BIOMARKERS FOR DETERMINING BREAST CANCER BONE METASTASIS

Номер: US20130260399A1
Автор: Byrum Stephanie, Suva Larry, Washam Charity
Принадлежит: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSA

The invention provides biomarkers and biomarker profiles that discriminate between a subject with breast cancer that has metastasized to the bone and a subject with breast cancer that has not metastasized to the bone. In particular, the biomarker and biomarker profile include polypeptides present or present at higher levels in the circulatory system of subjects having breast cancer bone metastasis compared to subjects having breast cancer without bone metastasis. 18-. (canceled)9. A method for detecting breast cancer bone metastasis in a subject with breast cancer , the method comprising:a. obtaining a biological sample from the subject; i. an amino acid sequence consisting of SEQ ID No: 2,', 'ii. an amino acid sequence that is at least 80% identical to SEQ ID No: 2, or', 'iii. an amino acid sequence of SEQ ID No: 2 with conservative amino acid substitutions, 'b. determining whether'}c. is present in the biological sample; andd. identifying the subject as having bone metastasis when an amino acid sequence of step (b) is present in the sample.10. A method for detecting breast cancer bone metastasis in a subject with breast cancer , the method comprising:a. obtaining a biological sample from the subject;b. determining whether the biological sample has the biomarker profile comprising SELDI polypeptide peaks M4260.92 Da, M4133.27 Da, and M4740.39 Da, andc. identifying the subject as having bone metastasis when SELDI polypeptide peaks M4260.92 Da, M4133.27 Da are present and SELDI polypeptide peak M4740.39 Da is absent in the sample.11. The method of claim 10 , further comprising detecting one or more of the SELDI polypeptide peaks selected from the group consisting of M11386.2 Da claim 10 , M11452.8 Da claim 10 , M 11525.9 Da claim 10 , M11629.0 Da claim 10 , M 11679.7 Da claim 10 , M11728.2 Da claim 10 , M11894.2 Da claim 10 , and M39096.5 Da.12. The method of claim 9 , wherein the presence of an amino acid sequence of step (b) is determined using Surface Enhanced ...

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Номер записи: 24
24-10-2013 дата публикации

Method Of Mass Spectrometry And A Mass Spectrometer

Номер: US20130277548A1
Автор: Brown Jeffery M., Claude Emmanuelle, Denny Richard, McDowall Mark, MURRAY Paul, Neeson Kieran, Towers Mark W.
Принадлежит:

The present invention relates to a method of mass spectrometry, an apparatus adapted to perform the method and a mass spectrometer. More particularly, but not exclusively, the present invention relates to a method of mass spectrometry comprising the step of associating parent and fragmentation ions from a sample by measuring the parent and fragmentation ions from two or more different areas of the sample and identifying changes in the number of parent ions between the areas in the sample, and corresponding changes in the number of fragmentation ions between the two areas. 1. A method of mass spectrometry comprising the steps of:providing a sample; exciting a first spot in the first area on the surface of the sample to produce a first set of parent analyte ions;', 'determining the mass to charge ratio of at least some of the ions from the first set of parent analyte ions as a function of ion mobility of the ions;', 'exciting a second spot in the first area on the surface to produce a second set of parent analyte ions;', 'determining the ion mobility of at least some of the ions of the second set of parent analyte ions;', 'fragmenting at least a portion of the second set of parent analyte ions to produce a set of fragment ions;', 'determining the mass to charge ratio of at least some of the ions from the set of fragment ions as a function of the ion mobility of the ions from the second set of parent analyte ions;', 'associating the set of fragment ions with the first set of parent analyte ions by comparison of the ion mobility of the ions from the first set of parent analyte ions with the ion mobility of the ions from the second set of parent analyte ions associated with the fragment ions;, 'performing a method of association of parent analyte ions from a first area of the sample with fragment ions from the first area of the sample comprising the steps ofperforming the method of association for at least a second area of the sample; andassigning at least one ion from ...

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Номер записи: 25
14-11-2013 дата публикации

MASS SPECTROMETRY METHOD, ION PRODUCTION DEVICE, AND MASS SPECTROMETRY SYSTEM

Номер: US20130299692A1
Автор: Kinoshita Kazumasa, Nakatani Yoshimasa, Noritake Yuka, Shida Yasuo, Shimada Haruo
Принадлежит:

A mass spectrometry method of the present invention is such that a sample is heated to generate a gas and an ion that is produced from the gas is introduced into a mass spectrometer by using DART so that mass spectrometry is conducted. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. (canceled)12. (canceled)13. A mass spectrometry method , comprising:heating a sample to generate a gas;producing an ion from the gas by using DART; andintroducing the ion into a mass spectrometer.14. The mass spectrometry method as claimed in claim 13 , wherein the heating of the sample includes applying a voltage to a resistance heating wire.15. The mass spectrometry method as claimed in claim 14 , wherein the heating of the sample further includes putting the sample into a pot wrapped with the resistance heating wire.16. The mass spectrometry method as claimed in claim 14 , wherein the heating of the sample further includes attaching the sample to the resistance heating wire.17. A mass spectrometry method claim 14 , comprising:heating a sample;producing an ion from the sample by using DART; andintroducing the ion into a mass spectrometer.18. The mass spectrometry method as claimed in claim 17 , wherein the heating of the sample includes attaching the sample to the resistance heating wire and applying a voltage to the resistance heating wire.19. An ion production device claim 17 , comprising:a heating device configured to heat a sample to generate a gas; anda DART ion source configured to produce an ion from the gas.20. The ion production device as claimed in claim 19 , wherein the heating device includes a pot configured to put the sample therein claim 19 , the pot is wrapped with a resistance heating wire claim 19 , and the heating device further includes a voltage applying device configured to apply a voltage to the resistance heating wire.21. The ion production device as claimed in claim 19 , ...

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Номер записи: 26
19-12-2013 дата публикации

MASS SPECTROMETRY DATA PROCESSING DEVICE

Номер: US20130338935A1
Автор: SUMIYOSHI Takashi, WATANABE Jun
Принадлежит:

In the case where a peak on a mass spectrum is saturated due to, for example, signal saturation in a detector or an amplifier provided downstream thereof, a data processor performs fitting with a Gaussian function using data included in the rising part and the falling part (range A) of the peak which are not affected by the saturation, to thereby obtain a desired approximate peak shape B. Then, a mass spectrum in which the saturated peak is replaced with the approximate peak thus obtained is created, the mass-to-charge ratio of the peak top is calculated for this mass spectrum, and this mass spectrum is then displayed on a display screen. Moreover, an extracted ion chromatogram is created on the basis of information on mass-to-charge ratio to intensity of this modified mass spectrum and displayed. Accordingly, even in the case where peak saturation occurs, the accuracy of the mass-to-charge ratio for the peak is improved, and the accuracy of compound identification and compound structure estimation using a mass spectrum or quantitative properties using a peak area value (integral value) are improved. 1. A mass analysis data processing apparatus that processes data collected by a mass spectrometer , comprising:a) a peak waveform estimating section for estimating, for a peak waveform having a saturated peak top on a mass spectrum based on the data, a peak waveform shape without the saturation on a basis of data in a slope portion of a bottom of the peak waveform; andb) an approximate spectrum creating section for creating a mass spectrum using the peak waveform shape estimated by the peak waveform estimating section, instead of the peak having the saturated peak top.2. The mass analysis data processing apparatus according to claim 1 , whereinthe peak waveform estimating section estimates the peak waveform shape without the saturation according to Gaussian distribution on the basis of the data in the slope portion of the bottom of the peak waveform having the saturated ...

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Номер записи: 27
26-12-2013 дата публикации

IONIZATION METHOD, MASS SPECTROMETRY METHOD, EXTRACTION METHOD, AND PURIFICATION METHOD

Номер: US20130341279A1
Автор: Arakawa Ryuichi, Otsuka Yoichi
Принадлежит:

To achieve soft ionization more easily when a slight amount of substance is ionized under an atmosphere pressure. An ionization method for a substance contained in a liquid, including: supplying the liquid to a substrate from a probe and forming a liquid bridge made of the liquid containing the substance dissolved therein, between the probe and the substrate; oscillating the probe; and generating an electric field between an electrically conductive portion of the probe in contact with the liquid and an ion extraction electrode. 1. An ionization method for a substance contained in a liquid , comprising:(i) supplying the liquid onto a substrate from a probe and forming a liquid bridge made of the liquid containing the substance, between the probe and the substrate; and(ii) generating an electric field between an electrically conductive portion of the probe in contact with the liquid and an ion extraction electrode.2. The ionization method according to claim 1 , wherein one end of the probe is oscillated in a direction that intersects with an axis of the probe.3. The ionization method according to claim 1 , wherein a position of the one end of the probe is different between the (i) supplying and forming and the (ii) generating.4. The ionization method according to claim 1 , wherein claim 1 , in the (ii) generating claim 1 , the liquid forms a Taylor cone at an end of the probe.5. The ionization method according to claim 1 , wherein claim 1 , in the (ii) generating claim 1 , part of the liquid escapes as charged droplets from the end.6. The ionization method according to claim 5 , wherein the charged droplets escape from the Taylor cone.7. The ionization method according to claim 5 , wherein the charged droplets cause a Rayleigh fission.8. The ionization method according to claim 1 , wherein the probe includes a plurality of flow paths.9. The ionization method according to claim 1 , wherein the probe includes a protrusion.10. The ionization method according to claim 9 , ...

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Номер записи: 28
09-01-2014 дата публикации

Ion generation using wetted porous material

Номер: US20140008529A1
Автор: HE Wang, Jiangjiang Liu, Nicholas E. Manicke, Qian Yang, Robert Graham Cooks, Zheng Ouyang
Принадлежит: PURDUE RESEARCH FOUNDATION

The invention generally relates to systems and methods for mass spectrometry analysis of samples. In certain embodiments, the invention provides a mass spectrometry probe including at least one porous material connected to a high voltage source, in which the porous material is discrete from a flow of solvent.

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Номер записи: 29
09-01-2014 дата публикации

Method for predicting whether a cancer patient will not benefit from platinum-based chemotherapy agents

Номер: US20140012514A1
Автор: Grigorieva Julia, Röder Heinrich, Röder Joanna
Принадлежит:

A testing method for identification whether a cancer patient is a member of a group or class of cancer patients that are not likely to benefit from administration of a platinum-based chemotherapy agent, e.g., cisplatin, carboplatin or analogs thereof, either alone or in combination with other non-platinum chemotherapy agents, e.g., gemcitabine and paclitaxel. This identification can be made in advance of treatment. The method uses a mass spectrometer obtaining a mass spectrum of a blood-based sample from the patient, and a computer operating as a classifier and using a stored training set comprising class-labeled spectra from other cancer patients. 1. A method for guiding treatment of a cancer patient , comprising the steps of:a) obtaining a blood-based sample from the patient;b) obtaining a mass-spectrum of the blood-based sample with the aid of a mass spectrometer;c) in a programmed computer, performing predefined pre-processing steps on the mass spectrum, obtaining integrated intensity values of selected features at one or more predefined m/z ranges in the spectrum after the pre-processing steps are performed and comparing the integrated intensity values with a training set comprising class-labeled spectra from blood-based samples other cancer patients and classifying the mass spectrum with a class label, andd) if the class label is Poor or the equivalent, the patient is predicted, in advance, to not benefit from treatment in the form of administration of a platinum-based chemotherapy agent and is thereby guided towards a treatment regimen not containing platinum agents.2. The method of claim 1 , wherein in step d) if the class label is Poor or the equivalent claim 1 , the patient is predicted to not benefit from treatment in the form of administration of a combination of a non-platinum chemotherapy agent and a platinum-based chemotherapy agent and the patient is guided towards treatment regimen not containing platinum agents.3. The method of claim 1 , wherein ...

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Номер записи: 30
30-01-2014 дата публикации

METHOD AND MARKER FOR THE DIAGNOSIS OF A BILE DUCT STRICTURE AND OF A CHOLANGIOCELLULAR CARCINOMA IN BILE

Номер: US20140027283A1
Автор: Mischak Harald
Принадлежит: MOSAIQUES DIAGNOSTICS AND THERAPEUTICS AG

A method for the diagnosis of a benign or malignant bile duct stricture and/or of a CCC, comprising the step of determining at least three polypeptide markers in a body fluid, wherein the polypeptide marker belongs to those markers which in table 1 and/or table 2 a and/or b are characterized by values for the molecular mass and the migration time. 1. A process for the differential diagnosis between a benign or malignant bile duct stricture and a choledocholithiasis , comprising the step of measuring the presence or absence or amplitude of at least three polypeptide markers in a sample of body fluid , wherein said polypeptide markers are selected from the markers characterized in Table 1 by values for the molecular masses and migration times.2. The process according to claim 1 , wherein an evaluation of the markers is effected by means of the reference values stated in Table 1.3. A process for the differential diagnosis between a cholangiocellular carcinoma and primary sclerosing cholangitis claim 1 , comprising the step of measuring the presence or absence or amplitude of at least three polypeptide markers in a sample of body fluid claim 1 , wherein said polypeptide markers are selected from the markers characterized in Table 2a by values for the molecular masses and migration times if the sample is a non-bile sample claim 1 , and said polypeptide markers are selected from the markers characterized in Table 2b by values for the molecular masses and migration times if the sample is a bile sample.4. The process according to claim 3 , wherein an evaluation of the markers is effected by means of the reference values stated in Tables 2a and 2b.5. The process according to claim 3 , wherein at least five claim 3 , at least six claim 3 , at least eight claim 3 , at least ten claim 3 , at least 20 or at least 50 polypeptide markers as defined in are used.6. The process according to claim 3 , wherein a urine sample is used as said non-bile sample.7. The process according to ...

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Номер записи: 31
30-01-2014 дата публикации

Sampling of confined spaces

Номер: US20140027630A1
Автор: Brian D. Musselman
Принадлежит: IonSense Inc

In various embodiments of the invention, a cargo container can be monitored at appropriate time intervals to determine that no controlled substances have been shipped with the cargo in the container. The monitoring utilizes reactive species produced from an atmospheric analyzer to ionize analyte molecules present in the container which are then analyzed by an appropriate spectroscopy system. In an embodiment of the invention, a sorbent surface can be used to absorb, adsorb or condense analyte molecules within the container whereafter the sorbent surface can be interrogated with the reactive species to generate analyte species characteristic of the contents of the container.

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Номер записи: 32
06-02-2014 дата публикации

Diagnosis and Treatment of Alzheimer's Disease

Номер: US20140037658A1
Автор: Blennow Kaj, Brinkmalm Gunnar, Halim Adnan, Larson Göran, NILSSON Jonas, Portelius Erik, Zetterberg Henrik
Принадлежит:

Methods are provided for the prevention, treatment and diagnosis of Alzheimer's disease, based on the glycosylation pattern of amyloid-beta peptides in body fluids and tissues. 1. An in vitro method for diagnosing or prognosing Alzheimer's disease in a subject , or determining whether a subject is at increased risk of developing Alzheimer's disease , comprising:a. determining the amounts of Abeta peptide with O-linked Tyr10 glycosylation in a sample; andb. comparing said level to a reference value representing a known disease or health status, wherein a varied level in said sample relative to a said reference value representing a known health status indicates a diagnosis, or prognosis, or increased risk of Alzheimer's disease.2. The method according to claim 1 , wherein step a) comprises determining the amount of Abeta peptide with O-linked Tyr10 glycosylation relative to unglycosylated Abeta peptide.3. The method according to claim 1 , wherein step a) comprises determining the amount of unglycosylated Abeta peptide relative to the total amount of Abeta peptide for indirect determination of Tyr10 glycosylated Abeta amount.4. The method according to wherein said sample is selected from the group consisting of cerebrospinal fluid claim 1 , serum claim 1 , urine claim 1 , whole blood claim 1 , lymphatic fluid claim 1 , plasma claim 1 , saliva claim 1 , cells claim 1 , tissue claim 1 , and material secreted by cells or tissues cultured in vitro.5. The method according to wherein said determination of the level of O-linked glycosylation at Tyr10 of Abeta or APP is performed by a method or combination of methods selected from the group consisting of Enzyme-linked immunosorbent assays (ELISAs) including Plasmon-enhanced colorimetric ELISA or other single molecule immunoassays using fluorescent lipid vesicles as enhancer elements claim 1 , mass spectrometry (MS) claim 1 , positron emission tomography-computed tomography (PET) claim 1 , magnetic resonance imaging (MRI) claim ...

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Номер записи: 33
13-03-2014 дата публикации

Method for analyzing glycan structure

Номер: US20140070086A1
Автор: Atsuhiko Toyama, Koji Ueda
Принадлежит: RIKEN Institute of Physical and Chemical Research, Shimadzu Corp

In order to provide an analysis method that is capable of determining a glycan structure with high detection sensitivity, a method of the present invention includes the steps of: carrying out triple quadrupole mass spectrometry at various values of CID energy; creating an energy-resolved profile including yield curves representing relationships between (i) a value of the CID energy and (ii) measured amounts of specific types of product ions; preparing a reference profile, and identifying a glycan structure of a test material by comparing the energy-resolved profile with the reference profile.

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Номер записи: 34
01-01-2015 дата публикации

FAST SWITCHING, DUAL POLARITY, DUAL OUTPUT HIGH VOLTAGE POWER SUPPLY

Номер: US20150001390A1
Автор: Collings Bruce Andrew, Dima Martian Daniel
Принадлежит: DH Technologies Development Pte. Ltd.

Systems, devices, circuits, and methods are provided for an improved mass spectrometry detection system that comprises an ion source and a detector that operate at opposite polarities. In some embodiments, the system can comprise a positive and negative multiplier, each of which can be configured to provide voltage to each of the ion source and the detector. In some embodiments, the system can comprise switches that allow the change between positive and negative polarities for the ion source or detector to occur quickly. A variety of embodiments of systems, devices, circuits, and methods in conjunction with the disclosures are provided. 1. A mass spectrometer system , comprising:an ion source;a detector configured to receive at least a portion of ions generated by the ion source;a positive multiplier configured to provide voltage to each of the ion source and the detector; anda negative multiplier configured to provide voltage to each of the ion source and the detector.2. The mass spectrometer system of claim 1 , further comprising one or more switches for controlling application of voltage to the detector and the ion source.3. The mass spectrometer system of claim 2 , wherein the one or more switches comprises a first switch configured to allow the positive multiplier to supply a positive voltage to the ion source when the negative multiplier supplies a negative voltage to the detector claim 2 , and to allow the positive multiplier to supply a positive voltage to the detector when the negative multiplier supplies a negative voltage to the ion source.4. The mass spectrometer system of claim 1 , wherein the voltage provided by at least one of the positive multiplier and the negative multiplier is in the range of about ±1 kV to about ±20 kV.5. A mass spectrometer system claim 1 , comprising:an ion source;a detector configured to receive at least a portion of ions generated by the ion source; andone or more switches coupled to one or more power supplies, the one or ...

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Номер записи: 35
07-01-2016 дата публикации

METHODS OF MONITORING FOR ADHERENCE TO ARIPIPRAZOLE THERAPY

Номер: US20160003800A1
Автор: McIntire Gregory L., Morris Ayodele
Принадлежит:

Methods for helping to monitor subject adherence with a prescribed antipsychotic drug treatment regimen are disclosed. 1. A method of treating schizophrenia in a subject in need thereof , the method comprising:identifying a subject who has been diagnosed with schizophrenia and who has been prescribed aripiprazole therapy;receiving a urine sample from the subject;determining a concentration of at least one aripiprazole metabolite in the urine;determining a concentration of at least one aripiprazole analyte in the urine; andrecommending a modification to the prescribed aripiprazole therapy if the concentration of the at least one aripiprazole analyte falls outside a normal distribution of the aripiprazole analyte,wherein the normal distribution of the aripiprazole analyte comprises normalized concentrations of the aripiprazole analyte associated with subjects known to be adherent to the aripiprazole therapy.2. The method of claim 1 , wherein the at least one aripiprazole analyte is one or more of: aripiprazole claim 1 , a dehydrogenated aripiprazole metabolite claim 1 , a hydroxylated aripiprazole metabolite claim 1 , an N-dealkylated aripiprazole metabolite claim 1 , a glucuronidated aripiprazole metabolite claim 1 , and a sulfated aripiprazole metabolite.3. The method of claim 2 , wherein the dehydrogenated aripiprazole metabolite is selected from the group consisting of: dehydroaripiprazole claim 2 , OPC-14857 claim 2 , OPC-3952 claim 2 , DM-1459 and DM-1460.4. The method of claim 2 , wherein the hydroxylated aripiprazole derivative is selected from the group consisting of DM-1451 claim 2 , DM-1452 claim 2 , DM-1454 claim 2 , DM-1458 claim 2 , DM-1459 claim 2 , DM-1460 claim 2 , OPC-14857 and DM-1431.5. The method of claim 2 , wherein the N-dealkylated aripiprazole derivative is selected from the group consisting of OPC3373 claim 2 , DCPP claim 2 , DM-1457 claim 2 , DM-1431 and OPC-3952.6. The method of claim 2 , wherein the glucuronidated aripiprazole metabolite ...

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Номер записи: 36
07-01-2016 дата публикации

ANALYSIS METHOD FOR ASSESSING STAGE OF PROSTATE CANCER, PROSTATE-CANCER STAGE ASSESSMENT METHOD, PROSTATE-CANCER DETECTION METHOD, AND TEST KIT

Номер: US20160003831A1
Автор: Nakagawa Hidewaki, Ueda Koji
Принадлежит:

Each of (i) an analysis method of the present invention for assessing a degree of progression of prostate cancer and (ii) a method of the present invention for assessing a degree of progression of prostate cancer includes the step of measuring an amount of neuropeptide Y in a sample derived from blood or urine obtained from a living organism. A method of the present invention for detecting prostate cancer includes the step of measuring respective amounts of neuropeptide Y and prostate-specific antigen in a sample derived from blood or urine obtained from a living organism. 1. An analysis method for assessing a degree of progression of prostate cancer , comprising the step of:measuring an amount of neuropeptide Y in a sample derived from blood or urine obtained from a living organism.2. The analysis method as set forth in claim 1 , further comprising the step of:measuring an amount of at least one of prostate-specific antigen, mRNA of TMPRSS2:ERG, and PCA3 RNA in a sample derived from blood or urine obtained from the living organism.3. The analysis method as set forth in claim 1 , further comprising the step of:measuring an amount of prostate-specific antigen in a sample derived from blood or urine obtained from the living organism.4. The analysis method as set forth in claim 1 , wherein:the amount of neuropeptide Y is measured by mass spectrometry or luminance assay.5. The analysis method as set forth in claim 1 , wherein:the sample is whole blood, a serum, or plasma.6. The analysis method as set forth in claim 1 , wherein:in the step of measuring the amount of neuropeptide Y, the sample is subjected to reversed phase extraction twice or more so that a fraction containing neuropeptide Y is obtained, and the amount of neuropeptide Y is measured in the fraction thus obtained.7. A method for detecting prostate cancer claim 1 , comprising the step of:measuring respective amounts of neuropeptide Y and prostate-specific antigen in a sample derived from blood or urine ...

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Номер записи: 37
07-01-2016 дата публикации

Photo or chemolabile conjugates for molecules detection

Номер: US20160003836A1
Автор: Jean-Philippe Ebran, Jonathan Stauber, Oleg Melnyk
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, IMABIOTECH, Universite De Lille 1 Sciences Et Technologies

The present invention relates to the field of the detection of molecules of interest in a sample, preferably by mass spectrometry. The present invention concerns a label compound, a molecule labeled with said compound (a conjugate), a method of detection of a molecule of interest (a target molecule) in a sample involving said conjugate, a kit to implement said method and a process for the preparation of the label.

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Номер записи: 38
07-01-2016 дата публикации

BIOMARKERS FOR THE PREDICTION OF PRETERM BIRTH

Номер: US20160003837A1
Автор: Feng Liping, MURTHA Amy
Принадлежит:

The present disclosure provides biomarkers useful for determining the risk of, prognosis of, and/or diagnosis of conditions such as preterm birth in a subject. 114.-. (canceled)15. A method of diagnosing preterm birth comprising:quantifying the amount of Progesterone Receptor Membrane Component 1 (PGRMC1) present in a biological sample derived from a pregnant subject, whereinthe subject is indicated as having an increased risk for preterm birth if the amount of the PGRMC1 biomarker is altered in the biological sample derived from the subject compared to a reference control.16. The method of claim 15 , wherein the biological sample is selected from the group consisting of tissues claim 15 , cells claim 15 , biopsies claim 15 , blood claim 15 , lymph claim 15 , serum claim 15 , plasma claim 15 , urine claim 15 , saliva claim 15 , mucus claim 15 , and tears.17. The method of claim 16 , wherein the biological sample comprises plasma and the amount of the PGRMC1 biomarker is greater in the plasma derived from the subject compared to the reference control.18. The method of claim 15 , wherein the PGRMC1 is a polypeptide and the quantifying is carried out by an assay comprising one or a combination of Western Blotting claim 15 , Array system claim 15 , affinity matrice claim 15 , and immunoassay.1925.-. (canceled)26. The method of claim 15 , wherein the biological sample is derived from the subject prior to 24 weeks of gestation.27. The method of claim 15 , wherein the biological sample is derived from the subject at about 28 weeks of gestation.28. The method of claim 15 , further comprising administering an appropriate prophylactic progesterone therapy if the subject is predicted as having an increased risk for preterm birth.29. A method for determining the efficacy of a preterm birth treatment comprising:determining a baseline value for the amount of Progesterone Receptor Membrane Component 1 (PGRMC1) present in a first biological sample comprising plasma derived from a ...

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Номер записи: 39
03-01-2019 дата публикации

METHOD AND APPARATUS FOR ION MOBILITY SEPARATIONS UTILIZING ALTERNATING CURRENT WAVEFORMS

Номер: US20190004011A1
Автор: Garimella Venkata BS, Hamid Ahmed M., Ibrahim Yehia M., Smith Richard D.
Принадлежит: BATTELLE MEMORIAL INSTITUTE

Methods and apparatuses for ion manipulations, including ion trapping, transfer, and mobility separations, using traveling waves (TW) formed by continuous alternating current (AC) are disclosed. An apparatus for ion manipulation includes a surface to which are coupled a first plurality of continuous electrodes and a second plurality of segmented electrodes. The second plurality of segmented electrodes is arranged in longitudinal sets between or adjacent to the first plurality of electrodes. An RF voltage applied to adjacent electrodes of the first plurality of electrodes is phase shifted by approximately 180° to confine ions within the apparatus. An AC voltage waveform applied to adjacent electrodes within a longitudinal set of the second plurality of segmented electrodes is phase shifted on the adjacent electrodes by 1°-359° to move ions longitudinally through the apparatus for separation. 1. An apparatus for ion manipulations , comprising:at least one surface;a first plurality of continuous electrodes coupled to the at least one surface and in electrical communication with a radiofrequency (RF) voltage source, wherein an RF voltage applied to adjacent electrodes of the first plurality of electrodes by the RF voltage source is phase shifted on the adjacent electrodes of the first plurality of electrodes by approximately 180°; anda second plurality of segmented electrodes coupled to the at least one surface and arranged in longitudinal sets between or adjacent to the first plurality of electrodes, the second plurality of segmented electrodes being further in electrical communication with an alternating current (AC) voltage source, wherein an AC voltage waveform applied to adjacent electrodes within a longitudinal set of the second plurality of segmented electrodes by the AC voltage source is phase shifted on the adjacent electrodes of the second plurality of electrodes by 1°-359°.2. The apparatus of claim 1 , further comprising a plurality of guard electrodes ...

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Номер записи: 40
07-01-2016 дата публикации

MULTI-REFLECTION MASS SPECTROMETER

Номер: US20160005585A1
Автор: GRINFELD Dmitry, Makarov Alexander
Принадлежит:

A multi-reflection mass spectrometer is provided comprising two ion-optical mirrors, each mirror elongated generally along a drift direction (Y), each mirror opposing the other in an X direction, the X direction being orthogonal to Y, characterized in that the mirrors are not a constant distance from each other in the X direction along at least a portion of their lengths in the drift direction. In use, ions are reflected from one opposing mirror to the other a plurality of times while drifting along the drift direction so as to follow a generally zigzag path within the mass spectrometer. The motion of ions along the drift direction is opposed by an electric field resulting from the non-constant distance of the mirrors from each other along at least a portion of their lengths in the drift direction that causes the ions to reverse their direction. 1. (canceled)2. A method of mass spectrometry comprising the steps of:injecting ions into a multi-reflection mass spectrometer comprising two ion-optical mirrors, each mirror elongated generally along a drift direction Y, each mirror opposing the other in an X direction, the X direction being orthogonal to Y direction;reflecting the ions from one mirror to the other, generally orthogonally to the drift direction, a plurality of times by turning the ions within each mirror whilst the ions proceed along the drift direction Y, wherein the distance between consecutive points in the X-direction at which the ions turn monotonously changes with Y during at least a part of the motion of the ions along the drift direction; anddetecting at least some of the ions during or after their passage through the multi-reflection mass spectrometer.3. The method of mass spectrometry of in which the multi-reflection mass spectrometer further comprises one or more electrically biased compensation electrodes extending along at least a portion of the drift direction claim 1 , each electrode being located in or adjacent a space between the mirrors.4. ...

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Номер записи: 41
03-01-2019 дата публикации

INTELLIGENTLY CONTROLLED SPECTROMETER METHODS AND APPARATUS

Номер: US20190006160A1
Автор: Osgood Mark A, Wu Ching
Принадлежит: Excellims Corporation

The present invention relates to improving the ability of a hyphenated instrument to analyze a sample benefiting from having the first instrument's analysis of the same sample. A fast switching mechanism can be used as the interface between an ion mobility spectrometer (IMS) and a mass spectrometer (MS) such that the obtained IMS spectrum is converted into a timing diagram that controls the vacuum inlet's size dynamically during analysis of a neutral and/or charged chemical and/or biological species such that a smaller pumping system can be used. 1. A vacuum inlet for a mass spectrometer comprising , a mechanism that controls a vacuum inlet structure for the mass spectrometera) to maintain the mass spectrometer at a pressure by dynamically controlling the size of the vacuum inlet at a first state; andb) to pulse the inlet structure according to a timing diagram to allow some part of a sample to enter the mass spectrometer, by temporarily changing the inlet structure to a second state;wherein each of the inlet structure states involves opening or closing the vacuum inlet to a particular size.2. The apparatus in claim 1 , further comprises an ion mobility spectrometer that is outside the vacuum inlet structure.3. The apparatus in claim 2 , wherein the timing diagram is generated by the ion mobility spectrometer.4. The apparatus of claim 1 , wherein the mechanism that controls a vacuum inlet structure includes driving component(s) that are used for maintaining the pressure and/or for pulsing to allow samples to enter the mass spectrometer.5. The apparatus of claim 4 , wherein the driving component(s) are selected from: magnetic actuator claim 4 , piezoelectric actuator claim 4 , mechanical actuator.6. The apparatus of claim 1 , wherein dynamically controlling the size of the vacuum inlet is performed by deforming a conductive elastomer.7. The apparatus of claim 6 , wherein the conductive elastomer is deformed by compressing claim 6 , expanding claim 6 , rotating and/or ...

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Номер записи: 42
08-01-2015 дата публикации

DIAGNOSTIC AND PROGNOSTIC BIOMARKERS FOR CANCER

Номер: US20150008314A1
Автор: LIU Jinbo, Sessler Daniel I.
Принадлежит: THE CLEVELAND CLINIC FOUNDATION

A method for detecting cancer from a biological sample previously withdrawn from the subject, in a subject includes determining in vitro the level of at least one biomarker in the biological sample. The at least one biomarker includes a phospholipid or a free fatty acid. A level of the at least one biomarker that is 2-fold greater than the level of at least one biomarker in a control is indicative of cancer in the subject. 1. A method for detecting cancer in a subject from a biological sample previously withdrawn from the subject , the method comprising determining in vitro the level of at least one biomarker in the biological sample , the at least one biomarker including a phospholipid or a free fatty acid , wherein a level of the at least one biomarker that is at least about 2-fold greater than the level of at least one biomarker in a control is indicative of cancer in the subject.2. The method of claim 1 , wherein the biological sample is selected from the group consisting of whole blood claim 1 , plasma and serum.3. The method of claim 2 , wherein the biological sample is about 0.5 ml of blood.4. The method of claim 1 , wherein the level of the at least one biomarker in the subject is determined using mass spectroscopy.5. The method of claim 4 , wherein the level of the at least one biomarker in the subject is determined using electrospray ionization tandem mass spectroscopy.6. The method of claim 1 , wherein the free fatty acid is at least one of linoleic acid (LA) or arachidonic acid (AA).7. The method of claim 1 , wherein the free fatty acid is at least one of a hydroxyeicosatetraenoic acid (HETE) or a hydroxyoctadecadienoic acid (HODS).8. The method of claim 7 , wherein the HETE is selected from the group consisting of 15-HETE claim 7 , 12-HETE claim 7 , 11-HETE and 5-HETE.9. The method of claim 1 , wherein the phospholipid is a lysophosphatylcholine (LPC) or HODE-PC.10. The method of claim 9 , wherein the LPC is LPC-C16.11. The method of claim 7 , wherein ...

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Номер записи: 43
08-01-2015 дата публикации

APPARATUS AND METHOD FOR SAMPLING OF CONFINED SPACES

Номер: US20150008315A1
Автор: Musselman Brian D.
Принадлежит: IONSENSE, INC

In various embodiments of the invention, a cargo container can be monitored at appropriate time intervals to determine that no controlled substances have been shipped with the cargo in the container. The monitoring utilizes reactive species produced from an atmospheric analyzer to ionize analyte molecules present in the container which are then analyzed by an appropriate spectroscopy system. In an embodiment of the invention, a sorbent surface can be used to absorb, adsorb or condense analyte molecules within the container whereafter the sorbent surface can be interrogated with the reactive species to generate analyte species characteristic of the contents of the container. 1. An atmospheric ionization sorbent module (AISM) system to analyze a cargo comprising:a container with a volume, where the container can be substantially confined on all sides, where the container includes at least a distal end and a proximal end;the atmospheric ionization sorbent module (AISM) including a sorbent surface and a region in front of the sorbent surface, where the AISM is in connection with one or both the distal end and the proximal end; andone or more fans to introduce ambient air to the region in front of the sorbent surface.2. The system of claim 1 , where the one or more fans are adapted to re-circulate air through the volume.3. The system of claim 1 , where the one or more fans one or both blow and suck air from one or more regions within the container across the sorbent surface.4. The system of claim 1 , where one or more tubes located in the container connect the AISM to one or both the distal end and the proximal end.5. The system of claim 4 , where the one or more fans are adapted to draw ambient air into the one or more tubes.6. The system of claim 4 , further comprising a partition claim 4 , where the partition separates the proximal end from the distal end claim 4 , where the proximal end has a first atmosphere claim 4 , where the distal end has a second atmosphere ...

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Номер записи: 44
14-01-2016 дата публикации

Clinical decision support (cds) for radiotherapy in prostate cancer

Номер: US20160008629A1
Автор: Carolina Ribbing, Katrin Bitter
Принадлежит: Koninklijke Philips NV

A system ( 30 ) and method for detecting or predicting toxicity induced by radiation therapy. A device ( 34 ) is configured for determining polypeptide biomarkers present in a urine sample. At least one processor ( 36 ) is programmed to detect or predict radiation toxicity based on one or more polypeptide biomarkers determined to be in the urine sample.

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Номер записи: 45
08-01-2015 дата публикации

P-CRESOL SULPHATE AS A BIOMARKER FOR HEALTHY AGING

Номер: US20150010673A1
Автор: Collino Sebastiano, Guy Philippe Alexandre, Martin Francois-Pierre, Rezzi Serge Andre Dominique, Roura Ivan Montoliu
Принадлежит:

Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose an in vitro method of diagnosing a lifestyle that allows to delay and/or avoid ageing related chronic inflammatory disorders using p-cresol sulphate as biomarker. 1. An in vitro method of diagnosing a lifestyle that allows to delay and/or avoid ageing related chronic inflammatory disorders , comprisingobtaining a urine sample from a subjectdetermining the level of p-cresol sulphate (PCS), in the sample, andcomparing the subject's PCS level to a predetermined reference value,wherein the predetermined reference value is based on an average urine PCS level in a control population, andwherein an elevated urine PCS level in the sample compared to the predetermined reference value indicates an increased likelihood to delay and/or avoid ageing related chronic inflammatory disorders.2. The method of claim 1 , further comprisingdetermining the level of phenylacetylglutamine (PAG)in the sample, andcomparing the subject's PAG level to a predetermined reference value,wherein the predetermined reference value is based on average urine PAG level in a control population, andwherein elevated urine PCS and PAG levels in the sample compared to the predetermined reference values indicate an increased likelihood to delay and/or avoid ageing related chronic inflammatory disorders.3. The method of to diagnose a lifestyle that permits healthy ageing.4. The method of to diagnose longevity.5. The method of to diagnose healthier gut microflora-host interactions.6. The method of claim 5 , wherein the healthier gut microflora-host interactions are diagnosed in elderly.7. The method of to diagnose a healthier lifestyle claim 1 , wherein the predetermined ...

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Номер записи: 46
14-01-2016 дата публикации

HEXANOYLGLYCINE AS BIOMARKER FOR THE PREDISPOSITON FOR WEIGHT GAIN AND OBESITY

Номер: US20160011203A1
Автор: Boulange Claire L., Collino Sebastiano, Dumas Marc-Emmanuel, Holmes Elaine, Kochhar Sunil, Martin Francois-Pierre, Montoliu Roura Ivan, Nicholson Jeremy, Rezzi Serge Andre Dominique
Принадлежит:

The present invention relates generally to the field of nutrition and health. In particular, the present invention relates to a new biomarker, its use and a method that allows it to diagnose the likelihood to resist diet induced weight gain, and/or to be susceptible to a diet induced weight gain. For example, the biomarker may be hexanoylglycine. 1. A biomarker in urine for detecting and/or quantifying the likelihood to resist high fat diet induced weight gain , wherein the biomarker is hexanoylglycine.2. (canceled)3. A method of diagnosing the likelihood of a subject to resist high fat diet induced weight gain , the method comprising:determining the level of hexanoylglycine in a urine sample previously obtained from a subject to be tested, andcomparing the subject's hexanoylglycine level to a predetermined reference value,wherein the predetermined reference value is based on an average hexanoylglycine level in urine in a control population, andwherein an increased hexanoylglycine level in the sample compared to the predetermined reference value indicates an increased likelihood to resist high fat diet induced weight gain.4. The method of claim 3 , further comprising the steps ofdetermining the level of at least one further biomarker selected from the group consisting of trimethylamine-N-oxide, isovalerylglycine, leucine, isobutyrate, acetate, guanidoacetate, sucrose, tartaric acid, hippuric acid and hydroxyphenylacetylglycine in the urine sample, andcomparing the subject's level of the at least one further biomarker to a predetermined reference value,wherein the predetermined reference value is based on average levels of that at least one further biomarker in a urine sample of a normal healthy control population, andwherein an increased isovalerylglycine, leucine, acetate, and/or a decreased trimethylamine-N-oxide, guanidoacetate, sucrose, tartaric acid, hippuric acid and/or hydroxyphenylacetylglycine level in the urine sample compared to the predetermined ...

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Номер записи: 47
14-01-2016 дата публикации

Mass spectrometry method using matrix

Номер: US20160011205A1
Автор: Shunsuke Izumi, Yuko Fukuyama
Принадлежит: Shimadzu Corp

The present invention provides amass spectrometry method using a matrix that is capable of easily and efficiently improving ionization efficiency in mass spectrometry without modifying a molecule to be analyzed, and a matrix for mass spectrometry. A mass spectrometry method using, as a matrix, a 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): where R is an alkyl group having 4 to 12 carbon atoms. The mass spectrometry method as described above, wherein an analysis object is a hydrophobic compound, particularly, a hydrophobic peptide.

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Номер записи: 48
11-01-2018 дата публикации

Method of Charge State Selection

Номер: US20180011055A1
Автор: Green Martin Raymond, Richardson Keith, Wildgoose Jason Lee
Принадлежит:

A method of mass spectrometry or ion mobility spectrometry is disclosed in which analyte ions of a desired charge state are isolated. The method comprises: separating analytes according to their electrophoretic mobility; ionising the analytes; and mass filtering the resulting analyte ions, wherein the mass to charge ratios of the ions transmitted by a mass filter are varied as a function of the electrophoretic mobility and according to a predetermined relationship such that substantially only ions having said desired charge state are transmitted by the mass filter. 1. A method of mass spectrometry or ion mobility spectrometry comprising:separating analytes in a separator such that analytes having different electrophoretic mobilities elute from the separator at different times;ionising the separated analytes as they elute from the separator so as to form analyte ions that are separated from each other;transmitting the analyte ions to at least one device that manipulates the ions; andvarying the operation of the at least one device based on the elution time of the analytes from the separator.2. The method of claim 1 , wherein the at least one device comprises a gas phase ion mobility separator that accumulates the analyte ions and periodically releases them into an ion mobility separation region of the ion mobility separator as a packet of ions; and wherein the frequency and/or duty cycle at which the packets of ions are released into the ion mobility separator is varied as a function of elution time of the electrophoretic mobility separator.3. The method of claim 2 , wherein for a given packet of ions that is released into the separation region of the ion mobility separator claim 2 , one or more further packet of ions is subsequently released into the separation region whilst at least some of the ions from said given packet of ions are still travelling through and/or being separated in the ion mobility separator.4. The method of claim 1 , wherein the at least one ...

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Номер записи: 49
12-01-2017 дата публикации

BEAM TRANSMISSION SYSTEM AND METHOD THEREOF

Номер: US20170011898A1
Автор: Boeker Jeff, CHEN Jiong, Hong Junhua, ZHANG Jin
Принадлежит:

A beam current transmission system and method are disclosed. The beam current transmission system comprises an extraction device, a mass analyzer, a divergent element, a collimation element and a speed change and turning element, wherein an analysis plane of the mass analyzer is perpendicular to a convergent plane of the extracted beam, and after entering an entrance, the beam is converged on a convergent point in a plane perpendicular to the analysis plane, and then is diverged from the convergent point and transmitted to the divergent element from an exit; the collimation element is used for parallelizing the beam in a transmission plane of the beam; and the speed change and turning element is used for enabling the beam to change speed so as to achieve a target energy while the beam is deflected so that the transmission direction of the beam changes by a first pre-set angle. Through the coordinated cooperation among a plurality of beam current optical elements, a relatively wider distribution can be formed in a vertical plane, so the invention is suitable to the processing of a wafer with a large size and also ensure better injection uniformity on the premise of avoiding energy contamination. 1. A beam transmission system , comprising an ion source and an extraction device , wherein the extraction device is for extracting a focused beam , the beam transmission system further including: a mass analyzer , a divergent element , a collimation element and a speed change and turning element provided next to the collimation element , the mass analyzer includes an entrance and an exit , wherein an analysis plane of the mass analyzer is perpendicular to a convergent plane of the extracted beam ,the mass analyzer is used for deflecting the beam in the analysis plane so that trajectories of ion beams with different mass-to-charge ratios are formed in the analysis plane, and after entering the entrance, the beam is converged on a convergent point in a plane perpendicular to ...

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Номер записи: 50
15-01-2015 дата публикации

FIRST AND SECOND ORDER FOCUSING USING FIELD FREE REGIONS IN TIME-OF-FLIGHT

Номер: US20150014522A1
Автор: Haufler Robert E., Loyd William Morgan
Принадлежит:

In some embodiments, a time of flight mass spectrometer can comprise an input orifice for receiving ions, a first ion accelerator stage for accelerating the ions along a first path, at least one ion reflector for receiving said accelerated ions and redirecting said ions along a second path different than the first path, a detector for detecting at least a portion of the ions redirected by said at least one ion reflector, and at least first and second field free drift regions disposed between said first acceleration stage and said detector, wherein said second field free region is disposed in proximity of the detector. In some embodiments, the lengths of the field free drift regions can be selected so as to provide 1st and 2nd order corrections of the time of flight of the ions with respect to variation in their initial positions. 1. A time of flight mass spectrometer , comprising:an input orifice for receiving ions,a first ion acceleration stage for accelerating the ions along a first path,a first ion reflector for receiving said accelerated ions and redirecting said ions along a second path different than the first path,a second ion reflector configured to redirect the ions propagating along the second path onto a third path,a detector for detecting at least a portion of the ions redirected by said second ion reflector,at least first and second field free drift regions disposed between said first acceleration stage and said detector, wherein said second field free region is disposed in proximity of the detector, anda second acceleration stage disposed between said first and second field free drift regions.2. The mass spectrometer of claim 1 , wherein said first and second field free drift regions are configured to correct for a spread in initial positions of ions entering the spectrometer relative to a reference position.3. The mass spectrometer of claim 2 , wherein the detector is positioned to receive the ions propagating along the third path.4. The mass ...

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Номер записи: 51
14-01-2016 дата публикации

Improved Lock Component Corrections

Номер: US20160013036A1
Автор: Wildgoose Jason Lee
Принадлежит:

A method of mass spectrometry is disclosed comprising initially calibrating or recalibrating a mass spectrometer at a time Tand at the same time measuring a time of flight or mass to charge ratio Mof one or more lockmass ions. The mass spectrometer is then operated at a subsequent time Tand the time of flight or mass to charge ratio Mof the one or more lockmass ions is measured at subsequent time T. The time of flight or mass to charge ratio of ions is then adjusted by or based upon the difference between the time of flight or mass to charge ratio Mof the one or more lockmass ions as measured at time Tand the time of flight or mass to charge ratio Mof the one or more lockmass ions as measured at time T. 1. A method of mass spectrometry comprising:{'sub': 0', '0, 'initially calibrating or re-calibrating a mass spectrometer at a time Tand at substantially the same time measuring a time of flight or mass to charge ratio Mof one or more lockmass ions;'}{'sub': '1', 'operating the mass spectrometer at a subsequent time T;'}{'sub': 1', '1, 'measuring the time of flight or mass to charge ratio Mof said one or more lockmass ions at said time T; and'}{'sub': 1', '1', '0', '0, 'adjusting the time of flight or mass to charge ratio of ions by or based upon the difference between the time of flight or mass to charge ratio Mof said one or more lockmass ions as measured at said time Tand said time of flight or mass to charge ratio Mof said one or more lockmass ions as measured at said time T.'}2. A method as claimed in claim 1 , wherein the step of initially calibrating or re-calibrating said mass spectrometer at said time Tcomprises performing a calibration routine to produce a calibration curve.3. A method as claimed in claim 2 , wherein said calibration curve corresponds to a curve of best fit which relates the measured mass to charge ratio or time of flight of a plurality of known ions with the actual or known mass to charge ratio or time of flight of said plurality of known ...

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Номер записи: 52
14-01-2021 дата публикации

Low Power Mass Analyzer and System Integrating Same For Chemical Analysis

Номер: US20210013018A1
Автор: Christian Noah
Принадлежит: Leidos, Inc.

A low power mass spectrometer (LPMS) includes an ionization source for generating an ionized sample beam; ion focusing optics for focusing the sample beam; and a static magnetic field region contained within an electric field-free drift region created between magnets acting as equipotential electrodes combined with a third equipotential surrounding electrode for receiving the focused sample beam and deflecting ions therein to different points on a detector array in accordance with an individual mass thereof. The LPMS operates at less than 1.2 Watts and has a physical footprint equal to or less than 12 inches at its largest length. 1. A handheld low power mass spectrometer for analyzing a sample's chemical contents comprising:an ion focusing component for focusing a sample ion beam containing individual sample ions having a range of masses to a focal point;a magnet assembly located at an output of the ion focusing component for creating a permanent magnetic field region beginning at the focal point of the ion focusing component for deflecting the focused sample ions using zero power; anda linear detector array for simultaneously detecting the deflected sample ions, wherein the linear detector array lies in the same plane as the focal point of the ion focusing component and further wherein individual sample ions are deflected 180 degrees from the focal point to different points along the linear detector array in accordance with an individual mass thereof;wherein the low power mass spectrometer has a physical footprint equal to or less than 12 inches at its largest length.2. The handheld low power mass spectrometer of claim 1 , further comprising an electrostatic energy filter.3. The handheld low power mass spectrometer of claim 1 , wherein the magnet assembly consists of first and second magnets connected to a surrounding yoke.4. The low power mass spectrometer of claim 1 , wherein the magnet assembly generates an approximately 1 Tesla static magnetic field.5. The ...

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Номер записи: 53
09-01-2020 дата публикации

LOW CROSS-TALK FAST SAMPLE DELIVERY SYSTEM BASED UPON ACOUSTIC DROPLET EJECTION

Номер: US20200013605A1
Автор: Morris Michael Raymond, Pringle Steven Derek, Richardson Keith, Sinclair Ian
Принадлежит: MICROMASS UK LIMITED

An ion source for a mass spectrometer is disclosed comprising an ultrasonic transducer which focuses ultrasonic energy onto a surface of a sample fluid without directly contacting the sample fluid. 127-. (canceled)28. An ion source for a mass spectrometer comprising:a transducer arranged and adapted to focus acoustic energy onto a surface of a sample fluid without said transducer directly contacting said sample fluid, wherein said transducer is arranged and adapted to eject one or more droplets from said sample fluid in a substantially controlled manner, and wherein said one or more droplets comprise a majority of un-ionised droplets; andan ionisation device arranged and adapted to ionise a volume of liquid comprising said one or more droplets ejected from said sample fluid by said transducer.29. An ion source as claimed in claim 28 , wherein said ionisation device is arranged and adapted to emit a stream of charged particles.30. An ion source as claimed in claim 29 , wherein said stream of charged particles emitted by said ionisation device comprise charged droplets and/or ions.31. An ion source as claimed in claim 28 , wherein said ionisation device comprises an Electrospray ion source claim 28 , an Atmospheric Pressure Chemical Ionisation (“APCI”) ion source claim 28 , an Impactor ion source wherein a sample is ionised upon impacting a target claim 28 , a Laser ion source claim 28 , an ultra-violet (“UV”) photoionisation device or an infra-red (“IR”) photoionisation device.32281. An ion source as claimed in claim claim 28 , wherein said ionisation device comprises an Electrospray ion source arranged and adapted to ionise said volume of liquid.33. An ion source as claimed in claim 28 , wherein said ionisation device is arranged and adapted to act as a source of secondary ionisation for said volume of liquid comprising said one or more droplets ejected from said sample fluid by said transducer.34. An ion source as claimed in claim 28 , wherein said transducer is ...

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Номер записи: 54
21-01-2016 дата публикации

Molecular markers in bladder cancer

Номер: US20160017434A1
Автор: Daphne Hessels, Franciscus Petrus Smit, Jacobus A. SCHALKEN
Принадлежит: Mdxhealth Research BV, NOVIOGENDIX RESEARCH BV

The Present invention relates methods for establishing the presence, or absence, of a bladder tumour and/or classification of the tumor according to the aggressiveness and/or establishing the prediction of prognosis and disease outcome for a human individual suffering from bladder cancer. Specifically, the present invention relates to methods for establishing the presence, or absence, of a bladder tumour in a human individual comprising: determining the expression of one or more genes chosen from the group consisting of ADAMTS12, ASPN, CDC20, COL10A1, CTHRC1, FAP, SFRP4, FOXM1, KRT6A, ANLN, CHI3L1, TPX2, CCNB2, IGF2BP2, INHBA, PDCD1LG2, transcript cluster 2526893, and transcript cluster 2526896 in a biological sample (tissue or bodyfluid) originating from said human individual; establishing up regulation of expression of said one or more genes as compared to expression of said respective one or more genes in a sample originating from said human individual not comprising tumour cells or tissue.

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Номер записи: 55
16-01-2020 дата публикации

EXTRACTION OF CANNABINOIDS, CURCUMINOIDS AND GINSENOSIDES

Номер: US20200016508A1
Автор: Hari V., Stockwell John
Принадлежит:

An example method for extracting phytochemical oil from plant parts includes freezing plant parts from at least one of Cannabis sativa, Curcuma longa, Panax ginseng, and Panax quinquefolius. The frozen plant parts are reduced to a plant powder, which is suspended in an aqueous buffer. The aqueous buffer containing the suspended plant powder is incubated with at least one pectinase and at least one cellulose. An aqueous phase of the incubated aqueous buffer is evaporated through steam heating to obtain a steam dried product. Phytochemical oil, which includes at least one of cannabinoids, curcuminoids, and ginsenosides, is extracted from the steam dried product. 1. A method for extracting phytochemical oil from plant parts , comprising:freezing plant parts from at least one of Cannabis sativa, Curcuma longa, Panax ginseng, and Panax quinquefolius;reducing the frozen plant parts to a plant powder;suspending the plant powder in an aqueous buffer;incubating the aqueous buffer containing the suspended plant powder with at least one pectinase and at least one cellulase;evaporating an aqueous phase of the incubated aqueous buffer through steam heating to obtain a steam dried product; andextracting phytochemical oil from the steam dried product, the phytochemical oil including at least one of cannabinoids, curcuminoids, and ginsenosides.2. The method of claim 1 , comprising claim 1 , prior to said freezing:sanitizing the plant parts, the sanitizing including at least one of washing, ultraviolet irradiation, and ozonolysis.3. The method of claim 2 , wherein said washing and disinfecting removes microorganisms from the plant parts.4. The method of claim 3 , wherein said reducing the frozen plant parts to a plant powder comprises pulverizing the frozen plant parts.5. The method of claim 1 , wherein:said freezing comprises cooling the plant parts with dry ice; andsaid reducing is performed while the plant parts are being cooled by the dry ice.6. The method of claim 1 , wherein ...

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Номер записи: 56
21-01-2016 дата публикации

Methods of Prognosing Preeclampsia

Номер: US20160018413A1
Автор: Alev Cantas, Butte Atul J., Ling Bruce Xuefeng, Miller Linda Liu, Sheng Guojun, Wen Qiaojun, Yang Ting
Принадлежит:

Preeclampsia peptide biomarkers are provided. Also provided are methods for using these biomarkers, including in prognosing or diagnosing preeclampsia in a pregnant individual by detecting these biomarkers in a sample from the pregnant individual. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.

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Номер записи: 57
21-01-2016 дата публикации

METHODS AND SYSTEMS FOR THE DIAGNOSIS AND TREATMENT OF ANDROGEN DISORDERS

Номер: US20160018420A1
Автор: Bhasin Shalender, Jasuja Ravi, Zakharov Mikhail N.
Принадлежит: FUNCTION PROMOTING THERAPIES, LLC

The technology described herein is directed to the diagnosis and treatment of androgen disorders and/or deficiencies, e.g. low testosterone. 1. A method of determining a free testosterone concentration in a biological sample comprising the following steps: i. A total SHBG concentration,', 'ii. A total testosterone concentration, and', 'iii. An albumin concentration;, 'a) Identifying in the biological sample'}b) Attributing at least two distinct interconverting microstates of an unliganded SHBG dimer having a first monomer and a second monomer;c) Calculating the free testosterone concentration in the biological sample using an ensemble allostery model encompassing readjustment of a first equilibria between the microstates upon binding of a first testosterone molecule to the first monomer and an allosteric interaction between two binding sites of the SHBG dimer.2. The method of claim 1 , wherein the step of identifying in the biological sample further comprises identifying in the biological sampleiv. A concentration of at least one non-testosterone steroid.3. The method of claim 2 , wherein the non-testosterone steroid is selected from the group consisting of an estradiol steroid claim 2 , an estrone steroid claim 2 , and a dihydrotestosterone steroid.4. The method of claim 1 , wherein the step of identifying in the biological sample further comprises identifying in the biological sampleiv. at least one analyte concentration.5. The method of claim 1 , wherein the step of identifying in the biological sample further comprises measuring in the biological sample using at least one analytical method.6. The method of claim 5 , wherein the analytical method is selected from the group consisting of an immunoassay and a mass spectrometry-based assay.7. The method of claim 1 , wherein the method further comprises the step of:d) using the free testosterone concentration to diagnose a medical disease in men, women, and children.8. The method of claim 7 , wherein the medical ...

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Номер записи: 58
03-02-2022 дата публикации

Desorption ion source with dopant-gas assisted ionization

Номер: US20220037142A1
Автор: Christoph Hauke Manfred BOOKMEYER, Jens Soltwisch, Klaus Dreisewerd
Принадлежит: Bruker Daltonics GmbH and Co KG, WESTFAELISCHE WILHELMS UNIVERSITAET MUENSTER

Disclosed is a device to generate ions from a deposited sample, comprising: A chamber which is arranged and designed to keep the deposited sample in a conditioned environment comprising a dopant gas, A desorption device which is arranged and designed to desorb the deposited sample in the chamber using an energy burst, An ionization device which, for the purpose of ionization, is arranged and designed to irradiate the desorbed sample in the chamber using coherent electromagnetic waves or expose it to an electric discharge, a plasma, or light of an arc discharge lamp with broadband emission spectrum, which are chosen such that the dopant gas is receptive to them, and An extraction device which is arranged and designed to extract ions from the desorbed sample and transfer them into an analyzer. Disclosed is also a method which is preferably conducted on such a device.

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Номер записи: 59
17-01-2019 дата публикации

METHODS OF DETECTING REVERSE TRIIODOTHYRONINE BY MASS SPECTROMETRY

Номер: US20190019660A1
Автор: BANKS J. Fred, CHOU Peter P., MATT Noriya M.
Принадлежит:

Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample. 1. A method for determining the amount of reverse triiodothyronine (rT3) in a sample by mass spectrometry , said method comprising:a. ionizing rT3 from the sample to generate one or more rT3 ions detectable by mass spectrometry, wherein ionizing is by electrospray ionization (ESI);b. determining the amount of one or more rT3 ions by mass spectrometry; andc. determining the amount of rT3 in the body fluid sample from the amount of said rT3 ions determined in (b).2. The method of claim 1 , further comprising subjecting the rT3 from the sample to liquid chromatography prior to ionizing.3. The method of claim 2 , wherein liquid chromatography comprises high performance liquid chromatography (HPLC) claim 2 , reverse phase liquid chromatography (RPLC) claim 2 , reverse-phase high performance liquid chromatography (RP-HPLC) claim 2 , or high turbulence liquid chromatography (HTLC).4. The method of claim 2 , further comprising subjecting the sample to protein precipitation prior to liquid chromatography.5. The method of claim 4 , wherein said protein precipitation comprises organic solvent precipitation.6. The method of claim 4 , wherein said protein precipitation comprises methanol precipitation.7. The method of claim 1 , wherein said mass spectrometry is tandem mass spectrometry.8. The method of claim 1 , wherein further comprising enriching rT3 in the sample.9. The method of claim 8 , wherein enriching comprises liquid chromatography claim 8 , filtration claim 8 , centrifugation claim 8 , thin layer chromatography (TLC) claim 8 , electrophoresis including capillary electrophoresis claim 8 , affinity separations including immunoaffinity separations claim 8 , or extraction.10. The method of claim 1 , wherein ...

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Номер записи: 60
16-01-2020 дата публикации

In-vehicle biochemical sensors

Номер: US20200020514A1
Автор: Paul Timothy FANSON
Принадлежит: Toyota Motor Engineering and Manufacturing North America Inc

The devices, systems, and methods described herein generally relate to chemical profiling of an occupant in a vehicle. The devices, systems and methods described herein can detect enclosure-related chemicals, the enclosure-related chemicals including biochemicals expelled by one or more occupants. The enclosure-related chemicals can then be associated to an associated occupant of the one or more occupants. A biological profile can then be created for the associated occupant, the biological profile comprising medical information and historical information related to the enclosure-related chemicals.

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Номер записи: 61
22-01-2015 дата публикации

Systems and Methods for Sequential Windowed Acquisition Across a Mass Range Using an Ion Trap

Номер: US20150025813A1
Автор: Collings Bruce Andrew
Принадлежит:

Systems and methods are provided to perform sequential windowed acquisition of mass spectrometry data. A mass range and a mass window width parameter are received for a sample. A plurality of ions from the sample that are within the mass range are collected in an ion trap of a mass spectrometer. Two or more mass adjacent or overlapping windows are calculated to span the mass range using the mass window width parameter. Ions within each mass window are ejected from the ion trap. A mass spectrum is then detected from the ejected ions of the each mass window with a mass analyzer of the mass spectrometer, producing a collection of mass spectra for the mass range. The two or more mass windows can all have the same width, can all have different widths, or can have at least two mass windows with different widths. 1. A system for sequential windowed acquisition of mass spectrometry data , comprising:a mass spectrometer that includes an ion trap and a mass analyzer; and receives a mass range and a mass window width parameter for a sample,', 'instructs the mass spectrometer to collect in the ion trap a plurality of ions from the sample that are within the mass range,', 'calculates two or more adjacent or overlapping mass windows that span a mass range using the mass window width parameter, and', 'instructs the mass spectrometer to eject ions within each mass window of the two or more adjacent or overlapping mass windows from the ion trap and detect a mass spectrum from the ejected ions of the each mass window with the mass analyzer, producing a collection of mass spectra for the mass range., 'a processor in communication with the mass spectrometer that'}2. The system of claim 1 , wherein the two or more adjacent or overlapping mass windows have the same width.3. The system of claim 2 , wherein processor instructs the mass spectrometer to eject ions within each mass window of the two or more adjacent or overlapping mass windows from the ion trap by using a different waveform ...

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Номер записи: 62
25-01-2018 дата публикации

MASS SPECTROMETRIC DETERMINATION OF EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID

Номер: US20180024151A1
Автор: GOLDMAN Scott M., NEIDICH Julie A.
Принадлежит:

The invention relates to the detection of DHA and EPA. In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods. 1. A method for determining the amount of one or more omega-3 fatty acids in a sample by mass spectrometry , the method comprising:(i) ionizing said one or more omega-3 fatty acids from the sample to generate one or more ions detectable by mass spectrometry;(ii) determining the amount of said one or more omega-3 fatty acids ions by mass spectrometry; and(iii) relating the amount of DHA ions to the amount of one or more omega-3 fatty acids in the sample, wherein said one or more omega-3 fatty acids comprises docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA).2. The method of claim 1 , wherein said ionizing comprises atmospheric pressure chemical ionization (APCI).3. The method of claim 2 , wherein said APCI is in negative ionization mode.4. The method of claim 1 , wherein said ionizing comprises electrospray ionization (ESI).5. The method of claim 1 , wherein mass spectrometry comprises tandem mass spectrometry.6. The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 327.2±0.5.7. The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 301.2±0.5.8. The method of claim 1 , wherein said sample comprises human serum or plasma.9. The method of claim 1 , wherein said sample is subjected to a hydrolyzing agent prior to ionization.10. The method of claim 8 , wherein said hydrolyzing agent is an acid.11. The method of claim 1 , wherein said one or more omega-3 fatty acids is subjected to liquid/liquid extraction prior to ionization.12. The method of claim 1 , wherein said one or more omega-3 fatty acids is purified by liquid chromatography prior to ionization.13. The method of claim 12 , wherein said liquid chromatography comprises high performance liquid ...

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Номер записи: 63
24-01-2019 дата публикации

Apparatus for and method of mass analysis

Номер: US20190025174A1
Автор: masahiro Sakuta
Принадлежит: Hitachi High Tech Science Corp

Disclosed is an apparatus for and a method of mass analysis in which a presence of an accessory substance which is difficult to be analyzed can be recognized visually and clearly. The apparatus for mass analysis analyzes a sample containing a substance to be measured and includes: a display unit; a memory unit storing a theoretical peak obtained by calculation with respect to a region of a mass spectrum of the substance; a matching degree calculation unit calculating a matching degree from multiple peaks that each of the mass spectrum of the sample in the region and the theoretical peak have; a matching degree displaying control unit displaying the matching degree on the display unit; and a superimposition displaying control unit displaying the mass spectrum of the sample and the theoretical peak in a superimposed way in a manner that is consistent with a mass-to-charge ratio.

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Номер записи: 64
24-01-2019 дата публикации

DETERMINATION OF ORGANIC SILICON IN HYDROCARBONACEOUS STREAMS

Номер: US20190025220A1
Автор: Al-Sabawi Mustafa, Mitchell Lindsay A.
Принадлежит:

Systems and methods are provided for determining the organic silicon content of petroleum fractions, such as refinery fractions. This can be achieved in part based on performing solvent-enhanced selective filtration on a hydrocarbonaceous sample to substantially remove inorganic silicon from the sample while retaining at least a substantial portion of the organic silicon in the sample. After the solvent-enhanced selective filtration, the organic silicon content of the filtered sample can be determined. The ability to determine the organic silicon content of a sample can be used to identify crude fractions and/or refinery fractions that may cause contamination problems within a refinery while reducing or minimizing the occurrence of false positive tests that could result from detection of inorganic silicon. 1. A method for determining the silicon content of a hydrocarbonaceous sample , comprising:mixing a hydrocarbonaceous sample with an aromatic solvent to form a mixture, the mixture comprising about 20 wt % to about 80 wt % of an aromatic solvent relative to a weight of the mixture;performing a solids removal process on the mixture suitable for removing particles having a particle size of about 1.0 μm or larger to form a reduced solids mixture; andcharacterizing the silicon content of the hydrocarbonaceous sample using a detection method comprising inductively coupled plasma.2. The method of claim 1 , wherein characterizing the silicon content of the hydrocarbonaceous sample comprises:separating a first fraction comprising a majority of the aromatic solvent and a second fraction comprising a majority of the silicon content from the reduced solids mixture; andcharacterizing the silicon content of the second fraction.3. The method of claim 2 , wherein separating a first fraction comprising a majority of the aromatic solvent from the reduced solids mixture comprises performing a separation based on distillation claim 2 , evaporation claim 2 , or a combination thereof. ...

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Номер записи: 65
04-02-2016 дата публикации

2-AAA as a Biomarker and Therapeutic Agent for Diabetes

Номер: US20160030373A1
Автор: Clish Clary, Gerszten Robert, WANG Thomas
Принадлежит:

Methods for treating a glucose-related metabolic disorder (e.g., diabetes) comprising administration of 2-aminoadipic acid (2-AAA) to subjects in need thereof. Also described are methods for predicting a subject's risk of developing a glucose-related metabolic disorder, and to methods for selecting and monitoring a treatment for a glucose-related metabolic disorder (e.g., diabetes). 19.-. (canceled)10. A method for determining risk of developing diabetes in a subject , the method comprising:determining a level of 2-aminoadipic acid (2-AAA) in a test sample from the subject;comparing the level of 2-AAA in the test sample to a reference level; anddetermining the subject has an increased risk of developing diabetes when the test sample has an increased level of 2-AAA as compared to the reference level.11. The method of claim 10 , wherein the test sample comprises serum or plasma from the subject.12. The method of claim 10 , wherein the subject has normal glucose tolerance.13. The method of claim 10 , further comprising selecting a treatment based on the level of 2-aminoadipic acid in the test sample.14. The method of claim 13 , further comprising administering the selected treatment to the subject.15. The method of claim 13 , wherein the treatment comprises administering to the subject an effective amount of at one or more additional anti-diabetes compound selected from the group consisting of acarbose claim 13 , miglitol claim 13 , metformin claim 13 , phenformin claim 13 , buformin claim 13 , repaglinide claim 13 , nateglinide claim 13 , tolbutamide claim 13 , chlorpropamide claim 13 , tolazamide claim 13 , acetohexamide claim 13 , glyburide claim 13 , glipizide claim 13 , glimepiride claim 13 , gliclazide claim 13 , troglitazone claim 13 , rosiglitazone claim 13 , pioglitazone claim 13 , peptide analogs claim 13 , glucagon-like peptide I (GLP1) and analogs thereof claim 13 , GLP agonists claim 13 , vildagliptin sitagliptin; dichloroacetic acid; amylin claim 13 , ...

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Номер записи: 66
29-01-2015 дата публикации

CITRULLINATED BRAIN AND NEUROLOGICAL PROTEINS AS BIOMARKERS OF BRAIN INJURY OR NEURODEGENERATION

Номер: US20150031048A1
Автор: Everett Allen Dale, Jin Zhicheng, Van Eyk Jennifer E.
Принадлежит: THE JOHNS HOPKINS UNIVERSITY

The present invention relates to the field of biomarkers. More specifically, the present invention relates to biomarkers useful in diagnosing brain injury or neurodegeneration. In one embodiment, a method for diagnosing brain injury in a patient comprises the steps of (a) obtaining a sample from the patient; (b) determining the ratio of citrullinated to unmodified arginine residues at one or more arginine residues of one or more brain injury biomarker proteins; and (c) correlating the ratio to a patient having brain injury or to a patient not having brain injury, thereby providing the diagnosis. 1. A method for diagnosing brain injury in a patient comprising the steps of:a. obtaining a sample from the patient;b. determining the ratio of citrullinated to unmodified arginine residues at one or more arginine residues of one or more brain injury biomarker proteins; andc. correlating the ratio to a patient having brain injury or to a patient not having brain injury, thereby providing the diagnosis.2. The method of claim 1 , wherein the sample is selected from the group consisting of blood claim 1 , peripheral blood claim 1 , serum claim 1 , plasma claim 1 , cerebrospinal fluid claim 1 , urine claim 1 , saliva claim 1 , stool and synovial fluid.3. The method of claim 1 , wherein the sample is blood claim 1 , plasma serum claim 1 , cerebrospinal fluid (CSF) claim 1 , or urine.4. The method of claim 3 , wherein the sample is CSF.5. The method of claim 3 , wherein the sample is blood.6. The method of claim 3 , wherein the sample is serum.7. The method of claim 1 , wherein the determining step is accomplished using multiple reaction monitoring mass spectrometry (MRM-MS).8. The method of claim 1 , wherein the determining step is accomplished using an immunoassay.9. The method of claim 1 , wherein the one or more brain injury biomarker proteins is neurogranin (NRGN) claim 1 , myelin basic protein (MBP) claim 1 , glial fibrillary acid protein (GFAP) claim 1 , peptidylarginine ...

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Номер записи: 67
29-01-2015 дата публикации

Phylogenetic Analysis of Mass Spectrometry or Gene Array Data for the Diagnosis of Physiological Conditions

Номер: US20150031069A1
Автор: Abu-asab Mones, Amri Hakima, Chaouchi Mohamed
Принадлежит:

A universal data-mining platform capable of analyzing mass spectrometry (MS) serum proteomic profiles and/or gene array data to produce biologically meaningful classification; i.e., group together biologically related specimens into clades. This platform utilizes the principles of phylogenetics, such as parsimony, to reveal susceptibility to cancer development (or other physiological or pathophysiological conditions), diagnosis and typing of cancer, identifying stages of cancer, as well as post-treatment evaluation. To place specimens into their corresponding clade(s), the invention utilizes two algorithms: a new data-mining parsing algorithm, and a publicly available phylogenetic algorithm (MIX). By outgroup comparison (i.e., using a normal set as the standard reference), the parsing algorithm identifies under and/or overexpressed gene values or in the case of sera, (i) novel or (ii) vanished MS peaks, and peaks signifying (iii) up or (iv) down regulated proteins, and scores the variations as either derived (do not exit in the outgroup set) or ancestral (exist in the outgroup set); the derived is given a score of “1”, and the ancestral a score of “0”—these are called the polarized values. Furthermore, the shared derived characters that it identifies are potential biomarkers for cancers and other conditions and their subclasses. 1. A method of quantifying progression of disease in an individual comprising:a) obtaining a first blood serum specimen, wherein the first blood serum specimen is a diseased specimen comprising cells from at least one mammal afflicted with a disease;b) obtaining a second blood serum specimen, wherein the second blood serum specimen is an outgroup comprising cells from at least one mammal not afflicted with the disease.c) determining m/z values by mass spectrometry for proteins contained in the diseased specimen and the outgroup;d) extracting an absolute minimum and an absolute maximum for each m/z value determined for the outgroup, wherein ...

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Номер записи: 68
29-01-2015 дата публикации

BIOMARKER PANELS, DIAGNOSTIC METHODS AND TEST KITS FOR OVARIAN CANCER

Номер: US20150031561A1
Автор: Bertenshaw Greg P., Seshaiah Partha, Yip Ping F.
Принадлежит: Vermillion, Inc.

Methods are provided for predicting the presence, subtype and stage of ovarian cancer, as well as for assessing the therapeutic efficacy of a cancer treatment and determining whether a subject potentially is developing cancer. Associated test kits, computer and analytical systems as well as software and diagnostic models are also provided. 1. A method of determining the ovarian cancer status of a subject , comprising the steps of:measuring the level of CA-125, HE4 and one or more biomarkers selected from the group consisting of IL-2 receptor alpha (IL-2Ra), Alpha-1-Antitrypsin (AAT), C-Reactive Protein (CRP), YKL-40, Cellular Fibronectin (cFib), prostasin, Tissue Inhibitor of Metalloproteinases 1 (TIMP-1), IL-8, IL-6, Vascular Endothelial Growth Factor B (VEGF-B), Matrix Metalloproteinase-7 (MMP-7), calprotectin, Insulin-like Growth Factor-Binding Protein 2 (IGFBP-2), Lectin-Like Oxidized LDL Receptor 1 (LOX-1), neuropilin-1, TNFR2, and MPIF-1 in a biological sample obtained from the subject; andcorrelating the measurements with ovarian cancer status.2. The method of claim 1 , further comprising measuring the level of Cancer Antigen 72-4 (CA-72-4).3. The method of claim 1 , wherein the ovarian cancer status is the presence claim 1 , absence claim 1 , or risk of ovarian cancer.4. The method of further comprising:managing subject treatment based on the status.5. The method of claim 4 , wherein managing subject treatment is selected from the group consisting of ordering more tests claim 4 , performing surgery claim 4 , and taking no further action.6. The method of further comprising:measuring the level of said biomarkers after subject management;correlating the measurements with ovarian cancer status; anddetermining if subject management resulted in a change in ovarian cancer status.7. The method of claim 1 , wherein measuring is selected from detecting the presence or absence of the biomarkers claim 1 , quantifying the amount of biomarkers claim 1 , and qualifying the ...

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Номер записи: 69
02-02-2017 дата публикации

Elemental Analysis of Organic Samples

Номер: US20170031033A1
Автор: MAKAROV Alexander A., Schwieters Johannes
Принадлежит:

A method of imaging analyte elements in an organic sample includes providing the sample as a layer on a substrate and reacting the sample on the substrate to produce one or more volatile products that leave the sample while the one or more elements remain in the sample. A majority of the sample layer by weight is removed from the substrate by the reaction and the remaining sample layer is enriched in the one or more elements which are not spatially disturbed by the reaction. The method including subsequently detecting the one or more elements in the concentrated sample layer using an imaging elemental analyzer. 1. A method of imaging one or more analyte elements in an organic sample , comprising:providing the sample as a layer on a substrate;reacting the sample on the substrate to produce one or more volatile products that leave the sample and enter the gas phase, whilst the one or more analyte elements remain in the sample and are spatially disturbed by the reaction on average not more than the spatial resolution of the imaging analysis, whereby a majority of the sample layer by weight is removed from the substrate by the reaction and the remaining sample layer is enriched in the one or more analyte elements; and subsequentlydetecting the one or more analyte elements in the enriched sample layer using an imaging elemental analyzer.2. The method of wherein the sample is a biological sample selected from tissue claim 1 , cells claim 1 , bacterial culture and bioorganic solution.3. The method of wherein the one or more elements are naturally occurring in the sample and/or have been introduced into the sample as elemental tags.4. The method of wherein the one or more elemental tags comprise one or more rare earth elements claim 3 , heavy metal elements and/or radioisotopes.5. The method of wherein the one or more elemental tags are attached to a binding member selected from a polypeptide claim 3 , polynucleotide claim 3 , antibody claim 3 , affibody claim 3 , and an ...

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Номер записи: 70
01-05-2014 дата публикации

Method for analyzing glycan structure

Номер: US20140117225A1
Автор: Atsuhiko Toyama, Koji Ueda
Принадлежит: RIKEN Institute of Physical and Chemical Research, Shimadzu Corp

In order to provide an analysis method that is capable of determining a glycan structure with high detection sensitivity, a method of the present invention includes the steps of: carrying out triple quadrupole mass spectrometry at various values of CID energy; creating an energy-resolved profile including yield curves representing relationships between (i) a value of the CID energy and (ii) measured amounts of specific types of product ions; preparing a reference profile, and identifying a glycan structure of a test material by comparing the energy-resolved profile with the reference profile.

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Номер записи: 71
05-02-2015 дата публикации

IONIZATION DEVICE, MASS SPECTROMETRY APPARATUS, MASS SPECTROMETRY METHOD, AND IMAGING SYSTEM

Номер: US20150034817A1
Автор: Kyogaku Masafumi, Otsuka Yoichi
Принадлежит:

A mass spectrometry apparatus includes a holding table that holds a specimen to be ionized, a probe that identifies a portion of the specimen to be ionized, an ion extraction electrode that extracts ions obtained by ionizing the specimen, a liquid supplying unit that supplies liquid to between the specimen and the probe to form a liquid bridge between the specimen and the probe, a vibrating unit that vibrates one of the probe and the holding table, an electric field generating unit that generates an electric field between the probe and the ion extraction electrode, a mass spectrometry unit that mass analyzes ions extracted by the ion extraction electrode, and a synchronization unit configured to synchronize a time at which ions are generated from the portion with a time at which the mass spectrometry unit measures the ions. 1. An ionization device comprising:a holding table configured to hold a specimen to be ionized;a probe configured to identify a portion of the specimen to be ionized;an ion extraction electrode configured to extract ions obtained by ionizing the specimen;a liquid supplying unit configured to supply liquid to between the specimen and the probe to form a liquid bridge between the specimen and the probe;a vibrating unit configured to vibrate one of the probe and the holding table;an electric field generating unit configured to generate an electric field between the probe and the ion extraction electrode; anda synchronization unit configured to perform at least one of the following two synchronization processes on the basis of vibration of one of the probe and the holding table:(i) synchronizing a time at which ions are generated from the portion with a time at which a mass spectrometry unit for mass analyzing the ions extracted by the ion extraction electrode measures the ions, and(ii) synchronizing vibration of the probe with vibration of the holding table.2. A mass spectrometry apparatus comprising:a holding table configured to hold a specimen to ...

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Номер записи: 72
04-02-2016 дата публикации

ION TRAP MASS ANALYZER APPARATUS, METHODS, AND SYSTEMS UTILIZING ONE OR MORE MULTIPLE POTENTIAL ION GUIDE (MPIG) ELECTRODES

Номер: US20160035555A1
Автор: HANSON CURTISS DWIGHT
Принадлежит:

In one aspect of the invention, an ion trap mass analyzer includes a variable- or multi-potential type ion guide (MPIG) assembly which has been pre-configured to produce a parabolic-type potential field. Each MPIG electrode has a resistive coating of designed characteristics. In one example the coating varies in thickness along the length of an underlying uniform substrate. The MPIG assembly can be a single MPIG electrode or an array of a plurality of MPIG electrodes. An array can facilitate delocalization for improved performance. This chemical modification of a uniform underlying substrate promotes cheaper and flexible instruments. The modified MPIG electrodes also allow miniaturization (e.g. micro and perhaps even nano-scale), which allows miniaturization of the instrument in which the single or plural modified MPIG electrode(s) are placed. This promotes portability and field use instead of limitation to laboratory settings. 1. An ion trap mass analyzer comprising:a. a housing; i. a feed wire;', 'ii. an insulator around the feed wire;', 'iii. an semi-conductive coating on the insulator, the semi-conductive coating having a predetermined variation in thickness;, 'b. an elongated multi-potential ion guide in the housing, the ion guide having a length and comprisingc. a potential supply electrode sub-assembly operatively connected to the ion guide;d. a reference electrode sub-assembly spaced from the supply electrode and along the ion guide;e. so that the predetermined variation in thickness of the semi-conductive coating on the ion guide and the supply and reference electrode sub-assemblies can be correlated to produce a customizable potential energy field relative to the length of the ion guide.2. The analyzer of wherein the insulator comprises a tubular member.3. The analyzer of wherein the tubular member comprises silica.4. The analyzer of wherein the semi-conductive coating comprises polymer.5. The analyzer of wherein the polymer provides resistance in the mega ...

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Номер записи: 73
01-02-2018 дата публикации

Techniques for controlling distance between a sample and sample probe while such probe liberates analyte from a sample region for analysis with a mass spectrometer

Номер: US20180033597A1
Автор: Andrey V. Liyu, Julia Laskin, Son N. Nguyen
Принадлежит: Battelle Memorial Institute Inc

A system includes a mass spectrometer and associated sample interfacing equipment. The sample interfacing equipment includes a platform structured to support a sample thereon, a fluid source, a high voltage source, a dispensing probe electrically coupled to the high voltage source and defining a fluid dispensing passage therethrough, a collection probe defining a collection passage therethrough, a sensing arrangement coupled to the dispensing probe, and control logic responsive to the sensing arrangement to control distance between the dispensing probe and the sample. The dispensing probe facilitates formation of one or more ionized sample analytes when dispensing the fluid through the dispensing passage proximate to the sample on the platform. The collecting probe receives at least some of the one or more ionized sample analytes to pass through the collection passage into the mass spectrometer for analysis.

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Номер записи: 74
01-02-2018 дата публикации

Apparatus and Methods for an Atmospheric Sampling Inlet for a Portable Mass Spectrometer

Номер: US20180033601A1
Автор: Qiu Yongqiang
Принадлежит: BaySpec, Inc.

Atmospheric sampling system designed to minimize cross-contamination between successive samples acquired by a portable, or handheld, mass spectrometer. Techniques to reduce the overall sample load on portable mass spectrometers having limited pumping capacity, such as capture pumps. Techniques and methods employing simple manual devices and micro vacuum pumps for purging the inlet system of a mass spectrometer. Reduction of cross-contamination between successive samples, permitting a portable mass spectrometer to correctly associate sample positives with specific sample sites or individuals. 1. A pulsed atmospheric sampling system for a portable mass spectrometer comprising:an inlet port for sample injection;a capillary line for transfer of said sample injection into said portable mass spectrometer;a pulse valve for controlling the time period of said sample injection through said capillary line;a rubber bulb used to manually evacuate the previously injected sample from said capillary line and said pulse valve.2. The sampling system of claim 1 , in which said rubber bulb is used to purge the sample inlet line by compressing said rubber bulb claim 1 , placing said rubber bulb over said inlet port claim 1 , and releasing said rubber bulb claim 1 , effectively purging said sample inlet line.3. The sampling system of claim 1 , in which an additional port with a corresponding cap or valve claim 1 , is connected to said capillary line.4. The sampling system of claim 3 , in which said rubber bulb is placed over said additional port claim 3 , and alternately compressed and released claim 3 , effectively purging said sample inlet line.5. The sampling system of claim 3 , in which said additional port is opened to atmosphere claim 3 , and said rubber bulb is placed over said inlet port and alternately compressed and released claim 3 , effectively purging said sample inlet line.6. A method for reducing sample cross-contamination from a pulsed atmospheric sampling system for a ...

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Номер записи: 75
01-02-2018 дата публикации

LOW TEMPERATURE PLASMA PROBE WITH AUXILIARY HEATED GAS JET

Номер: US20180033602A1
Автор: Berkout Vadym, Saul Thomas D.
Принадлежит:

A low temperature plasma probe, a mass spectrometry system, and a method for using a low temperature plasma probe are described. In an embodiment, a low temperature plasma probe includes an intake capillary that provides an ion flow from a sample surface to a mass spectrometer; at least one low temperature plasma tube that provides low temperature plasma gas; at least one heated gas tube that provides heated gas to the sample surface, where the heated gas enhances desorption and ionization of a sample on the sample surface. 1. A low temperature plasma probe , comprising:an intake capillary that provides an ion flow from a sample surface to a mass spectrometer;at least one low temperature plasma tube that provides low temperature plasma gas;at least one heated gas tube that provides heated gas to the sample surface, where the heated gas enhances desorption and ionization of a sample on the sample surface.2. The low temperature plasma probe of claim 1 , wherein the intake capillary is configured as a first electrode.3. The low temperature plasma probe of claim 1 , wherein the at least one low temperature plasma tube includes two low temperature plasma tubes disposed on an intake capillary outer surface claim 1 , and where a low temperature plasma tube end is flush with an entrance of the intake capillary.4. The low temperature plasma probe of claim 1 , wherein the at least one low temperature plasma tube includes an outer tube that is concentric with the intake capillary claim 1 , and where a gas is pumped through the outer tube claim 1 , the intake capillary configured as a first electrode and the outer tube configured as a second electrode.5. The low temperature plasma probe of claim 4 , wherein the at least one heated gas tube is concentric to the outer tube and the intake capillary.6. The low temperature plasma probe of claim 1 , wherein air is pumped through the at least one low temperature plasma tube.7. The low temperature plasma probe of claim 1 , where at ...

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Номер записи: 76
17-02-2022 дата публикации

Method for evaluating mass spectrometry device, method for calibrating mass spectrometry device, analysis method, mass spectrometry device, and mass spectrometry reagent

Номер: US20220051885A1
Автор: Katsuhiro Nakagawa, Kenichi OBAYASHI, Yukihiko KUDO
Принадлежит: Shimadzu Corp

A method for evaluating a mass spectrometry device includes: by a mass spectrometry device, performing mass spectrometry of an ester of phthalic acid and detecting a plurality of types of ions produced by dissociation of the ester of phthalic acid; and obtaining information concerning whether the mass spectrometry device is in a state suitable for analysis, based on a ratio of intensities of the plurality of types of ions detected.

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Номер записи: 77
31-01-2019 дата публикации

SYSTEMS AND METHODS FOR PERFORMING MULTIPLE PRECURSER, NEUTRAL LOSS AND PRODUCT ION SCANS IN A SINGLE ION TRAP

Номер: US20190035614A1
Автор: Cooks Robert Graham, Snyder Dalton
Принадлежит:

The invention generally relates to systems and methods for performing multiple precursor, neutral loss and product ion scans in a single ion trap. In certain aspects, the invention provides systems including a mass spectrometer having a single ion trap, and a central processing unit (CPU), and storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to apply at least one of the following ion scans to a single ion population in the single ion trap: multiple precursor ion scans, a plurality of segmented neutral loss scans, or multiple simultaneous neutral loss scans. 1. A system comprising:a mass spectrometer comprising a single ion trap; anda central processing unit (CPU), and storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to apply at least one of the following ion scans to a single ion population in the single ion trap: multiple precursor ion scans, a plurality of segmented neutral loss scans, or multiple simultaneous neutral loss scans.2. The system according to claim 1 , wherein the multiple precursor ion scans are applied sequentially to the single ion population.3. The system according to claim 1 , wherein the multiple precursor ion scans are applied simultaneously to the single ion population.4. The system according to claim 1 , wherein the ion scans are multiple precursor ion scans claim 1 , and the CPU is configured to cause the system to apply at least one additional scan to the single ion trap.5. The system according to claim 4 , wherein the at least one additional scan is a neutral loss scan.6. The system according to claim 5 , wherein the CPU causes the system to apply the neutral loss scan simultaneously with the multiple precursor ion scans.7. The system according to claim 5 , wherein the CPU causes the system to apply the neutral loss scan sequentially with the multiple precursor ion scans.8. The system according to claim 4 , wherein the at least one ...

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Номер записи: 78
31-01-2019 дата публикации

SYSTEMS AND METHODS FOR COLLISION INDUCED DISSOCIATION OF IONS IN AN ION TRAP

Номер: US20190035619A1
Автор: Cooks Robert Graham, Snyder Dalton
Принадлежит:

The invention generally relates to systems and methods for collision induced dissociation of ions in an ion trap. In certain aspects, the invention provides a system that includes a mass spectrometer having an ion trap, and a central processing unit (CPU). The CPU includes storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to generate one or more signals, and apply the one or more signals to the ion trap in a manner that all ions within the ion trap are fragmented at a same Mathieu q value. 1. A mass spectrometry system comprising:a mass spectrometer comprising an ion trap; anda central processing unit (CPU), and storage coupled to the CPU for storing instructions that when executed by the CPU cause the system to:generate one or more signals; andapply the one or more signals to the ion trap in a manner that all ions within the ion trap are fragmented at a same Mathieu q value.2. The system according to claim 1 , wherein claim 1 , the one or more signals comprises a radio frequency (RF) signal in which an amplitude of the RF signal ramps in a reverse direction from high amplitude to low amplitude.3. The system according to claim 2 , wherein the radio frequency (RF) signal is applied with a second signal that is a fixed frequency resonance excitation waveform.4. The system according to claim 3 , wherein the fixed frequency resonance excitation waveform is a supplementary alternating current (AC) signal.5. The system according to claim 4 , wherein an amplitude of the supplementary alternating current (AC) signal is varied as a function of time.6. The system according to claim 5 , wherein the amplitude of the supplementary alternating current (AC) signal is ramped from a high amplitude to a low amplitude.7. The system according to claim 1 , wherein claim 1 , the one or more signals comprises a radio frequency (RF) signal in which an amplitude of the RF signal ramps in a forward direction from low amplitude to high ...

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Номер записи: 79
30-01-2020 дата публикации

User interface for ion mobility separation device

Номер: US20200035472A1
Автор: Jakub Ujma, Keith George Richardson, Kevin Giles, Sandra Richardson
Принадлежит: Micromass UK Ltd

A method of controlling the operation of an ion mobility separation device is disclosed. The method comprises displaying to a user via a user interface a pool of modes of operation of the ion mobility separation device, wherein each one of the modes is selectable by the user for inclusion in an experiment, and the modes are displayed in a first area 202 of the user interface. The method comprises receiving, via the user interface, an indication from the user of a selection of one or more instance of each one of a plurality of the modes from the pool to be included in an experiment, and an indication from the user of a set of one or more parameters for controlling the ion mobility separation device in respect of one or more selected instances of a mode. The selected instances of modes are displayed in a sequence in a second area 204 of the user interface. The operation of the ion mobility separation device is controlled in accordance with the received indications.

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Номер записи: 80
11-02-2016 дата публикации

Fingerprinting for gas lift diagnostics

Номер: US20160041132A1
Автор: Michael C. Romer, Michael K. Johnson, Ted A. Long
Принадлежит: Individual

Method for determining the depth of entry of lift gas into a production tube of a gas lift well includes the step of determining the concentration of GCOI endogenous to formation gas produced from the production tube of a gas lift well and absent from gas used as the lift gas in said gas lift well versus time after a perturbation of the rate of introduction of said lift gas into said production tube via one or more gas lift valves. The invention includes a system configured for evaluating the performance of gas lift well, including a separator, a flow regulation device, a measurement device for measuring over time the concentration of a GCOI in production fluids, a data collection and storage device for recording data from the measurement device, and a computer program product embodied on a computer-readable medium for analyzing the data collected by the measuring device.

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Номер записи: 81
11-02-2016 дата публикации

IDENTIFICATION AND MONITORING OF MONOCLONAL IMMUNOGLOBULINS BY MOLECULAR MASS

Номер: US20160041184A1
Автор: Barnidge David R., Murray David L.
Принадлежит:

Disclosure herein are methods for determining whether or not an immunoglobulin is present above the polyclonal background level in a biological sample, and methods for determining whether an immunoglobulin contains a kappa or lambda light chain. These methods are useful for screening biological samples for the presence or absence of monoclonal gammopathy, and for diagnosing and monitoring monoclonal gammopathy in a subject. 1. A method of determining whether or not an immunoglobulin is present above the polyclonal background in a sample , the method comprising:a. providing a sample comprising variable region-containing immunoglobulins;b. subjecting the sample to a mass spectrometry technique to obtain a mass spectrum of the sample; andc. determining whether or not an immunoglobulin is present above the polyclonal background level by detecting the presence or absence of an M-protein peak in the mass spectrum.2. The method of claim 1 , wherein variable region-containing immunoglobulins are selected from immunoglobulin light chains claim 1 , immunoglobulin heavy chains claim 1 , and antigen binding fragments (Fabs) of immunoglobulins claim 1 , and mixtures thereof.3. The method of claim 1 , further comprising determining the presence or absence of monoclonal gammopathy in the sample based on the presence or absence of an M-protein peak in the mass spectrum.4. A method of diagnosing monoclonal gammopathy in a subject claim 1 , the method comprising:a. subjecting a subject sample comprising variable region-containing immunoglobulins to a mass spectrometry technique to obtain a mass spectrum;b. determining whether or not an immunoglobulin of the subject is present above the polyclonal background level by detecting the presence or absence of an M-protein peak in the mass spectrum; andc. diagnosing the presence or absence of monoclonal gammopathy in the subject based on whether or not an immunoglobulin is present above the polyclonal background.5. A method of monitoring a ...

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Номер записи: 82
12-02-2015 дата публикации

CHARACTERIZATION AND PREDICTION OF JET FUEL QUALITY

Номер: US20150041634A1
Автор: GAUGHAN Roger G., Gooding Beatrice M., Novak William J., QIAN Kuangnan, Quann Richard J., Riedinger William E.
Принадлежит: EXXONMOBIL RESEARCH AND ENGINEERING COMPANY

Systems and methods are provided for characterizing kerosene fractions in order to determine whether the fractions will satisfy a desired thermal breakpoint specification. Additionally, hydrotreating conditions can be determined that will result in a hydrotreated kerosene fraction that satisfies the desired thermal breakpoint specification. The hydrotreating conditions can be determined based on a model constructed from data corresponding to a plurality of reference samples. The model can include data for compositional groups within the reference samples. The data for compositional groups can be derived from Fourier transform ion cyclotron resonance mass spectrometry data or from another suitable characterization technique. 1. A method for preparing a jet fuel or kerosene product , comprising:measuring a thermal breakpoint for a plurality of kerosene fractions;measuring one or more of a sulfur content, a nitrogen content, an aromatics content, and an API gravity for the plurality of kerosene fractions;analyzing the plurality of kerosene fractions, using Fourier transform ion cyclotron resonance mass spectrometry, to determine weights for the plurality of compositional groups within each kerosene fraction;constructing a model for correlating a thermal breakpoint of a kerosene fraction with a) the one or more of a sulfur content, a nitrogen content, an aromatics content, and an API gravity for a kerosene fraction, and b) weights for a plurality of compositional groups in a kerosene fraction, the model being constructed based on the measured thermal breakpoints for the plurality of kerosene fractions, the measured values for the one or more of a sulfur content, a nitrogen content, an aromatics content, and an API gravity for the plurality of kerosene fractions, and the determined weights for the plurality of compositional groups for the plurality of kerosene fractions;determining, for a first kerosene fraction, a thermal breakpoint and the one or more of a sulfur ...

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Номер записи: 83
18-02-2021 дата публикации

Enclosed desorption electrospray ionization probes and method of use thereof

Номер: US20210045662A1
Автор: Chien-Hsun Chen, Livia Schiavinato Eberlin, Robert Graham Cooks, Zheng Ouyang, Ziqing Lin
Принадлежит: PURDUE RESEARCH FOUNDATION

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

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Номер записи: 84
15-02-2018 дата публикации

Physically Guided Rapid Evaporative Ionisation Mass Spectrometry ("REIMS")

Номер: US20180042582A1
Автор: Daniel Simon, Daniel Szalay, Emrys Jones, James Ian Langridge, Julia BALOG, Keith Richardson, Lajos Godorhazy, Michael Raymond MORRIS, Steven Derek Pringle, Zoltan Takats
Принадлежит: Micromass UK Ltd

A method is disclosed comprising obtaining physical or other non-mass spectrometric data from one or more regions of a target using a probe. The physical or other non-mass spectrometric data may be used to determine one or more regions of interest of the target. An ambient ionisation ion source may then used to generate an aerosol, smoke or vapour from one or more regions of the target.

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Номер записи: 85
06-02-2020 дата публикации

SYSTEMS AND METHODS FOR DISCOVERY AND ANALYSIS OF MARKERS

Номер: US20200041487A1
Автор: Greenquist Alfred, Sassi Alexander, Stults John T.
Принадлежит:

A business method for use in classifying patient samples. The method includes steps of collecting case samples representing a clinical phenotypic state and control samples representing patients without said clinical phenotypic state. Preferably the system uses a mass spectrometry platform system to identify patterns of polypeptides in said case samples and in the control samples without regard to the specific identity of at least some of said polypeptides. Based on identified representative patterns of the state, the business method provides for the marketing of diagnostic products using representative patterns. The present invention relates to systems and methods for identifying new markers, diagnosing patients with a biological state of interest, and marketing/commercializing such diagnostics. The present invention relates to systems and methods of greater sensitivity, specificity, and/or cost effectiveness. 120-. (canceled)21. A method of identifying a biological state of a subject , the method comprising:(a) obtaining a biological sample from the subject, wherein the biological sample is a fluid sample;(b) depleting the biological sample by removing highly abundant proteins to yield a depleted sample, wherein the highly abundant proteins are present in the biological sample at a concentration of at least 10 μg/mL;(c) assaying proteins in the depleted sample to detect a plurality of biomarkers and generate a biomarker pattern; and(d) processing the biomarker pattern using a trained classifier, wherein the trained classifier assigns one or more biological states to the biomarker pattern based on the concentration of at least one biomarker of the plurality of biomarkers to identify the biological state of the subject.22. The method of claim 21 , wherein the at least one biomarker of the plurality of biomarkers is present in the biological sample at a concentration of no more than 7 pg/ml.23. The method of claim 21 , wherein the at least one biomarker of the ...

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Номер записи: 86
18-02-2021 дата публикации

Systems and Methods for Using Variable Mass Selection Window Widths in Tandem Mass Spectrometry

Номер: US20210050197A1
Автор: Bonner Ronald F., Tate Stephen A.
Принадлежит:

Systems and methods are used to analyze a sample using variable mass selection window widths. A tandem mass spectrometer is instructed to perform at least two fragmentation scans of a sample with different mass selection window widths using a processor. The tandem mass spectrometer includes a mass analyzer that allows variable mass selection window widths. The selection of the different mass selection window widths can be based on one or more properties of sample compounds. The properties may include a sample compound molecular weight distribution that is calculated from a molecular weight distribution of expected compounds or is determined from a list of molecular weights for one or more known compounds. The tandem mass spectrometer can also be instructed to perform an analysis of the sample before instructing the tandem mass spectrometer to perform the at least two fragmentation scans of the sample. 1. A system for analyzing a sample using variable precursor mass selection window widths , comprising:a tandem mass spectrometer that includes a mass analyzer that allows variable precursor mass selection window widths across a mass range of a sample; and creating a list of molecular weights for known target compounds in the sample;', 'generating a molecular weight distribution from a database using the list of molecular weights, and', 'selecting narrow mass selection window widths for the known target compounds and selecting wider mass selection window widths for areas in between the known target compounds., 'a processor in communication with the tandem mass spectrometer that instructs the tandem mass spectrometer to perform at least two fragmentation scans of at least two variable precursor mass selection window widths with different precursor mass selection window widths across the mass range of the sample in a single scan of the mass range, wherein the different precursor mass selection window widths are calculated by'}2. The system of claim 1 , wherein the ...

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Номер записи: 87
18-02-2016 дата публикации

METHOD FOR DETECTING AND QUANTIFYING A TARGET ANALYTE IN A SAMPLE

Номер: US20160049284A1
Автор: Bonnel David, Hamm Grégory, Pamelard Fabien, STAUBER JONATHAN
Принадлежит:

The invention relates to a method for identifying and quantifying by mass spectrometry at least one target analyte in a sample, comprising the following steps: 1. A method for detecting and quantifying by mass spectrometry imaging at least one target analyte in a sample , comprising the following steps:a) depositing the sample containing at least one target analyte to be analyzed on a support for mass spectrometry imaging and depositing a known concentration of isotopically labeled target analyte, as an internal standard, on said sample;b) analyzing the sample by a mass spectrometry imaging technique so as to obtain a mass spectrum signal of said at least one target analyte and a mass spectrum signal of the isotopically labeled target analyte in said sample;c) weighting the signal associated with the mass spectrum of said at least one target analyte in said sample by an extinction coefficient specific to each of said at least one target analyte and to the sample, wherein said extinction coefficient corresponds to a ratio of the signal of the at least one target analyte to the signal of the isotopically labeled target analyte, or the inverse; andd) using each weighted signal of each of said at least one target analyte to determine the quantity and distribution of each of said at least one target analyte in the sample.2. The method according to claim 1 , wherein said at least one target analyte is a protein claim 1 , a peptide claim 1 , a lipid claim 1 , a metabolite or a small molecule.3. The method according to claim 1 , wherein the sample is an organic sample or an inorganic sample.4. The method according to claim 1 , wherein the sample is a biological sample.5. The method according to claim 1 , wherein said at least one target analyte is a protein and an enzymatic and/or chemical pretreatment of said protein is performed prior to detection step b) claim 1 , and wherein detection and quantification are carried out for at least one of the peptides resulting from ...

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Номер записи: 88
08-05-2014 дата публикации

Device for obtaining the ion source of a mass spectrometer using an ultraviolet diode and a cem

Номер: US20140124662A1
Автор: Hyun Sik Kim, Mo Yang, Seung Yong KIM
Принадлежит: Korea Basic Science Institute KBSI

The present invention relates to a device for obtaining the ion source of a mass spectrometer using an ultraviolet diode and a CEM module, having the purpose of inducing initial electron emission using a CEM module and by radiating ultraviolet photons emitted from the ultraviolet diode to the entrance of the CEM module to obtain a large amount of amplified electron beams from the exit and to produce electron beams the emission times of which are accurately controlled at low temperature and at low power. The present invention is characterized by a device for obtaining the ion source of a mass spectrometer using an ultraviolet diode and a CEM module, the device consisting essentially of: an ultraviolet diode emitting ultraviolet rays by means of supplied power; an electron multiplier inducing and amplifying the initial electron emission of ultraviolet photons from the ultraviolet diode and obtaining a large amount of electron beams from the exit; an electron condenser lens condensing the electron beams amplified by the electron multiplier; an ion trap mass separator ionizing gas sample molecules by the electron beams injected through the electron xondensing lens; and an ion detector detecting ions separated from the ion trap mass separator by mass spectrum, wherein the electron multiplier is a CEM module.

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Номер записи: 89
15-02-2018 дата публикации

Method and Apparatus for Chemical Ionization of a Gas Mixture

Номер: US20180047550A1
Автор: Breitenlechner Martin, Hansel Armin
Принадлежит:

A method and apparatus for chemical ionization of analyte gas particles in a carrier gas by introducing primary ions, characterized in that the primary and product ions are accelerated by a rotating electric field orthogonal to that direction () in which the ions are transported towards the exit () of the reaction volume (). This can, for example, reduce unwanted cluster formation without increasing the transport speed of the ions through the reaction chamber, which improves, for example, the product ion yield. The apparatus of the invention achieves this by means of N≧3 rod electrodes () to which N AC voltages U(t), . . . , U(t) with N different phase positions ascending in one sense of rotation φ, . . . , φare applied. 115-. (canceled)16. A method for chemical ionization of a gas mixture by ion-atom reactions or ion-molecule reactions , wherein the gas mixture comprises at least one main component or a carrier gas and one or more reactant gases or analytes , wherein ionization is carried out by reactions with primary ions additionally introduced into the gas mixture , such that product ions are generated from neutral atoms or molecules of the reactant gases , the method comprising: 'wherein the reaction volume comprises a first top surface, a second top surface and a lateral surface extending between the two top surfaces and wherein the central line extends from the first top surface to the second top surface and completely within the reaction volume;', '(a) introducing the gas mixture into a reaction volume having a central line parallel to an axial direction and orthogonal to a radial direction and a radial plane,'}(b) introducing primary ions suitable for the chemical ionization of the one or more reactant gas components into the reaction volume;(c) accelerating the primary ions and generated product ions in the reaction volume by means of a temporally periodic electric field E(x,t),{'sub': r', 'r, '(d) wherein E(x,t) has a radial field vector component E(Z,t) ...

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Номер записи: 90
15-02-2018 дата публикации

Rapid Evaporative Ionisation Mass Spectrometry ("REIMS") and Desorption Electrospray Ionisation Mass Spectrometry ("DESI-MS") Analysis of Swabs and Biopsy Samples

Номер: US20180047554A1
Автор: Daniel Simon, Daniel Szalay, Emrys Jones, James Ian Langridge, Julia BALOG, Keith Richardson, Lajos Godorhazy, Michael Raymond MORRIS, Steven Derek Pringle, Tamas Karancsi, Zoltan Takats
Принадлежит: Micromass UK Ltd

A method is disclosed comprising providing a biological sample on a swab, directing a spray of charged droplets onto a surface of the swab in order to generate a plurality of analyte ions, and analysing the analyte ions.

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Номер записи: 91
26-02-2015 дата публикации

METHOD FOR DIAGNOSING STOMACH CANCER USING CHANGE OF TRYPTOPHAN METABOLISM

Номер: US20150053852A1
Автор: Jung Byung Hwa, KIM Myung Soo, Lee Soo Hyun
Принадлежит: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

The present disclosure relates to stomach cancer diagnosis using tryptophan metabolism rate in one aspect, and it relates to an invention using change of tryptophan metabolism rate in a stomach cancer patient, which is different from a normal person. 1. A method for diagnosing stomach cancer , comprising:measuring concentrations of tryptophan, anthranilic acid, serotonin, kynurenine, indole sulfate, nicotinic acid and nicotinamide in blood collected from an evaluation subject; anddiagnosing to have stomach cancer possibility in the case that blood concentration of at least one selected from the group consisting of tryptophan, anthranilic acid, serotonin, kynurenine and indole sulfate is lower than that of blood concentration of a normal person, or in the case that blood concentration of at least one selected from the group consisting of nicotinic acid and nicotinamide is higher than that of blood concentration of a normal person.2. The method for diagnosing stomach cancer according to claim 1 , wherein the diagnosing is to diagnose to have stomach cancer possibility in at least one case selected from the group consisting of: decreasing of blood concentration ratio of anthranilic acid/tryptophan claim 1 , decreasing of blood concentration ratio of serotonin/tryptophan claim 1 , increasing of blood concentration ratio of kynurenine/tryptophan claim 1 , increasing of blood concentration ratio of nicotinic acid/tryptophan and increasing of blood concentration ratio of nicotinamide/tryptophan claim 1 , compared to a normal person.3. The method for diagnosing stomach cancer according to claim 1 , wherein the diagnosing is to diagnose to have stomach cancer possibility in at least one case selected from the group consisting of: concentration ratio of anthranilic acid/tryptophan of 0.0005 to 0.003 claim 1 , concentration ratio of serotonin/tryptophan of 0.003 to 0.006 claim 1 , concentration ratio of kynurenine/tryptophan of 0.01 to 0.04 claim 1 , concentration ratio of ...

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Номер записи: 92
25-02-2016 дата публикации

TRIFLUOROBORATE MASS SPECTROMETRIC TAGS

Номер: US20160052942A1
Автор: Huang Yumei
Принадлежит:

The invention provides compositions and methods for mass spectrometric (MS), organic synthesis, and applications of organo-trifluoroborate, for example, as mass tags for use in negative ion mode. When subject to MS fragmentation, organo-trifluoroborates preferentially undergo neutral losses of hydrogen fluoride (HF) or boron trifluoride (BF) molecules, transferring the negative charge to the rest of the molecule. Such a fragmentation pattern is used to detect and quantitate analytes of interest after derivatization with organo-trifluoroborates. 1. A method for mass spectrometric analysis , comprisingfragmenting organo-trifluoroborate or an analyte tagged with one or more organo-trifluoroborate mass tags comprising one or more trifluoroborate groups in negative ion mode to produce one or more negatively charged fragment ions and one or more molecules with no charge.2. The method of claim 1 , wherein the mass spectrometric analysis provides qualitative monitoring and/or analysis of the analyte.3. The method of claim 1 , wherein the mass spectrometric analysis provides quantitative monitoring and/or analysis of the analyte.4. The method of claim 1 , wherein the fragmentation energy is zero.6. The method of claim 5 , wherein one or more of the organo-trifluoroborate mass tags is labeled with one or more stable heavy isotopes selected from B claim 5 , B claim 5 , C claim 5 , C claim 5 , N claim 5 , N claim 5 , O claim 5 , O claim 5 , H and H isotopes at select atomic sites such that the total additional mass due to heavy isotope labeling is distributed between the reactive group claim 5 , the linker claim 5 , and trifluoroborate group to produce a set of isobaric and mass differential tags.7. The method of claim 1 , wherein one or more of the organo-trifluoroborate mass tags comprise two or more trifluoroborate groups claim 1 , which organo-trifluoroborate mass tags undergo fragmentation(s) in negative ion mode to produce one or more fragment ions that are mono-negative ...

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Номер записи: 93
25-02-2021 дата публикации

ADVANCED GLYCATION END PRODUCT ANALOGUES

Номер: US20210054012A1
Автор: NASH Robert James
Принадлежит: Phytoquest Limited

Disclosed are processes for the production of composition comprising one or more fructose amino acids, said process comprising the steps of: (a) providing plant material derived from a botanical source selected from plants of the families Solanaceae, Compositae, (Asteraceae), Guttiferae, Umbelliferae, Papaveraceae, Vitidaceae or Acanthaceae; (b) extracting one or more fructose amino acid(s) from said plant material; and optionally (c) detecting the presence and/or measuring the amount of said fructose amino acid(s) in the extract of step (b). 1. A process for the production of composition comprising one or more fructose amino acids , said process comprising the steps of:(a) providing plant material derived from a botanical source selected from plants of the families Solanaceae, Compositae (Asteraceae), Guttiferae, Umbelliferae, Papaveraceae, Vitidaceae or Acanthaceae;(b) extracting one or more fructose amino acid(s) from said plant material; and optionally(c) detecting the presence and/or measuring the amount of said fructose amino acid(s) in the extract of step (b).2Hypericum perforatum.. The process of wherein the botanical source comprises plants of the species3. The process of for the production of a pharmaceutical composition claim 1 , further comprising the step of formulating said extracted fructose amino acid(s) with a pharmaceutically-acceptable excipient or carrier.4. The process of for the production of a cosmetic composition claim 1 , further comprising the step of formulating said extracted fructose amino acid(s) with a cosmetically-acceptable excipient or carrier.5. The process of for the production of a nutraceutical composition claim 1 , further comprising the step of formulating said extracted fructose amino acid(s) with a nutraceutically-acceptable excipient or carrier.6. The process of wherein the amount and concentration of the extracted fructose amino acids is: (a) sufficient to treat AGE-mediated disease in a subject; (b) sufficient to produce ...

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Номер записи: 94
25-02-2016 дата публикации

ION MODIFICATION

Номер: US20160054263A1
Автор: Atkinson Jonathan, SHARP David
Принадлежит:

An ion mobility spectrometry method comprising determining whether a sample comprises ions having a first characteristic, and in the event that it is determined that the sample comprises ions having the first characteristic, applying thermal energy together with a radio frequency, RF, electric field to parent ions so as to obtain daughter ions having a second characteristic for inferring at least one identity for the parent ions based on the first characteristic and the second characteristic. 1. An ion mobility spectrometry method comprising:determining whether a sample comprises ions having a first characteristic;in the event that it is determined that the sample comprises ions having the first characteristic, applying thermal energy together with a radio frequency, RF, electric field to parent ions so as to obtain daughter ions having a second characteristic for inferring at least one identity for the parent ions based on the first characteristic and the second characteristic.2. The method of in which determining whether the sample comprises the ions having the first characteristic comprises applying one of an RF electric field and thermal energy claim 1 , to modify a first plurality of ions derived from the sample.3. The method of in which applying the thermal energy comprises applying thermal energy to a region of a spectrometer drift chamber for a selected period.4. The method of in which the spectrometer drift chamber comprises an electrode for applying the RF electric field claim 3 , and the thermal energy is localised within a selected distance of the electrode.5. The method of in which applying thermal energy together with the RF electric field comprises applying the thermal energy prior to applying the RF electric field.6. The method of further comprising determining a temperature of a region of a spectrometer drift chamber claim 1 , and only applying the thermal energy in the event that the temperature is less than a selected threshold temperature.7. The ...

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Номер записи: 95
25-02-2016 дата публикации

Prenatal Screening

Номер: US20160054293A1
Автор: Butler Stephen Andrew, Iles Raymond Kruse
Принадлежит:

The present invention relates to a method for screening maternal urine samples for changes in the pattern of mass spectral fingerprinting which have been found to be characteristic of fetal aneuploidies such as Down's Syndrome and have application for the screening of other fetal abnormalities and disorders of pregnancy including gestational trophoblastic diseases. 1. A method of detecting a disorder of pregnancy or fetal aneuploidy up to the second trimester comprising:subjecting a maternal urine sample from a pregnant woman to direct mass spectral analysis, andcomparing the patterns resulting from said analysis to mass spectral patterns obtained from normal pregnancies or non-aneuploid pregnancies to determine whether said patterns from said sample are indicative of a disorder of pregnancy or fetal aneuploidy.2. The method according to for detecting fetal aneuploidy up to the second trimester comprising:providing a maternal urine sample from a pregnant woman,subjecting the urine sample to direct mass spectral analysis, andcomparing the patterns resulting from said analysis to mass spectral patterns obtained from non-aneuploid pregnancies to determine whether said patterns from said sample are indicative of fetal aneuploidy.3. The method according to claim 1 , wherein the maternal urine sample is from a pregnant woman at between 7 and 16 weeks gestation.4. The method according to claim 1 , wherein the urine sample is diluted prior to direct mass spectral analysis.5. The method according to claim 1 , wherein the sample is subjected to direct mass spectral analysis without any prior processing.6. The method according to claim 1 , wherein the disorder of pregnancy is selected from Ectopic pregnancy claim 1 , Threatened Miscarriage claim 1 , Hyperemesis Gravidarum claim 1 , Gestational Trophoblastic Diseases claim 1 , insufficiency claim 1 , preeclampsia claim 1 , gestational diabetes claim 1 , obstetric cholestasis claim 1 , and recurrent miscarriage in both normal ...

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Номер записи: 96
25-02-2016 дата публикации

FUTURE CARDIAC EVENT BIOMARKERS

Номер: US20160054334A1
Автор: Biessen Ericus Anna Leonardus, Kraaijeveld Adriaan Otto, Van Berkel Theodorus Josephus Cornelis
Принадлежит:

The present invention relates to the identification of chemokine biomarkers predictive of future acute coronary syndromes including unstable angina pectoris (UAP). The present invention also identifies particular chemokines as potential therapeutic targets for intervention in cardiovascular diseases. 1. A method for identifying a subject at increased risk of a future acute cardiovascular syndrome or event , the method comprising:a) determining an amount of at least one compound selected from the group consisting of CCL3 (chemokine (C—C motif) ligand 3), CCL18 (chemokine (C—C motif) ligand 18), and CCL5 (chemokine (C—C motif) ligand 5) in a sample obtained from the subject by contacting the sample with:i) an antibody specific for CCL3, and quantifying the amount of CCL3 bound to the antibody,ii) an antibody specific for CCL18, and quantifying the amount of CCL18 bound to the antibody, oriii) an antibody specific for CCL5, and quantifying the amount of CCL5 bound to the antibody; andb) identifying the subject as having an increased risk of a future acute cardiovascular syndrome or event if the sample from the subject was determined to have greater CCL3, greater CCL5, or greater CCL18 relative to a subject with no risk.2. The method according to claim 1 , wherein the cardiovascular syndrome or event may comprise coronary artery disease claim 1 , atherosclerosis claim 1 , acute myocardial infarction claim 1 , arteriosclerosis claim 1 , unstable angina pectoris claim 1 , embolism claim 1 , deep vein thrombosis claim 1 , stroke claim 1 , congestive heart failure or arrhythmia.3. The method according to claim 1 , wherein the indication of an increased risk of a future acute cardiovascular syndrome or event may be used for monitoring the status and/or progression of said syndrome or event.4. The method according to claim 1 , wherein the indication of an increased risk of a future acute cardiovascular syndrome or event may be used for monitoring therapeutic regimes and/or ...

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Номер записи: 97
23-02-2017 дата публикации

PAPER CONE TIP, AND PAPER CONE SPRAY IONIZATION MASS SPECTROMETRY USING THE SAME

Номер: US20170053788A1
Автор: Cha Sangwon, Kim Purum
Принадлежит:

A paper cone tip, having a triangular-pyramidal shape with a vertex angle of 12.5° to 45°, and to a paper cone spray ionization mass spectrometry (PCSI MS) method using a paper cone tip, including: preparing the paper cone tip having a triangular-pyramidal shape; placing a measuring sample in the paper cone tip and locating the paper cone tip in front of a mass spectrometer; and adding a spraying solvent to the paper cone tip and applying a voltage thereto. The paper cone tip having a triangular-pyramidal shape is suitable for use in PCSI MS for the direct analysis of solid samples of raw materials, and the three-dimensional paper cone tip serves as a sample container, a solid-liquid extraction chamber, an analyte transport channel, and an electrospray tip. 1. A paper cone tip , having a triangular-pyramidal shape with a vertex angle of 12.5° to 45°.2. The paper cone tip of claim 1 , wherein the paper cone tip is formed by folding a circular-sector-shaped paper into quarters.3. The paper cone tip of claim 1 , wherein the paper cone tip has a volume of 19.5 microliters (μL) to 303 μL.4. The paper cone tip of claim 1 , wherein the paper is a weighing paper.5. The paper cone tip of claim 1 , wherein the paper cone tip is transparent.6. A paper cone spray ionization mass spectrometry method using a paper cone tip claim 1 , comprising:preparing the paper cone tip having a triangular-pyramidal shape with a vertex angle of 12.5° to 45°;placing a measuring sample in the paper cone tip and locating the paper cone tip in front of a mass spectrometer; andadding a spraying solvent to the paper cone tip and applying a voltage thereto.7. The method of claim 6 , wherein the spraying solvent is an alcoholic solvent.8. The method of claim 6 , wherein the paper cone tip serves as a sample container claim 6 , an extraction chamber claim 6 , a transport channel for an extracted analyte claim 6 , and an electrospray tip.9. The method of claim 6 , wherein the measuring sample is a solid ...

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Номер записи: 98
13-02-2020 дата публикации

Monitoring Ion Mobility Spectrometry Environment for Improved Collision Cross Section Accuracy and Precision

Номер: US20200051803A1
Автор: Farnoush Salarzaei, Jason Lee Wildgoose, Nicholas TOMCZYK, Steven Derek Pringle
Принадлежит: Micromass UK Ltd

A mass spectrometer is disclosed comprising an ion mobility separator 4 for separating ions according to their ion mobility, one or more first devices or stages arranged upstream of the ion mobility separator and a control system. The control system is arranged and adapted to monitor directly or indirectly the operating environment within the ion mobility separator 4 , and to control the operating environment within the ion mobility separator 4 based on the monitoring by controlling one or more gas flows to or within one or more of the one or more first devices or stages.

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Номер записи: 99
13-02-2020 дата публикации

Quantitation Throughput Enhancement by Differential Mobility Based Pre-Separation

Номер: US20200051805A1
Автор: Mikhail V. UGAROV
Принадлежит: Thermo Finnigan LLC

A system for analyzing a sample includes a source configured to generate ions from constituent components of the sample; a mobility separator configured to separate ions received from the source based on the mobility in a gas; a plurality of ion channels arranged adjacent to the plurality of exit apertures of the mobility separator such that ions from the mobility separator are directed to different channels according to their respective mobility; a mass analyzer configured to determine the mass-to-charge ratio of the ions; and a controller. The controller is configured to identify retention time windows with minimum overlap of ions with similar mobility and sets of ions within the retention time windows; adjust mobility separation parameters for specific sets of ions to optimize separation of compounds; and quantify a plurality of target analytes.

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Номер записи: 100