29-03-2018 дата публикации
Номер: US20180092177A1
Принадлежит:
A high intensity, ultra-short LED pulsing driver circuit is developed for use with a system designed to perform real-time time-resolved, transient recording of fluorescence. Details of the timing circuitry used to pulse the LED and to provide synchronized PMT gating and ADC trigger pulses are also developed. The LED pulses are intended for fluorophores with lifetimes on the order of about 1.6 ns or longer and gating is used to maintain the detector off or partially off during excitation, thereby maximizing the available detector gain without saturation of the detector by the excitation light. 1. A system effective for time-resolved , transient recording of fluorescent lifetimes , the system comprising:{'b': 101', '101', '102', '101', '102, 'a. a personal computer (PC) graphical interface (), operatively coupled to a microcontroller, wherein the PC () initiates a series of pulses from the microcontroller (), the PC () and microcontroller () collectively referred to as a microcontroller edge trigger;'}{'b': '102', 'b. the microcontroller ();'}{'b': 300', '304, 'claim-text': [{'b': '302', 'i. a signal shaping circuit (), configured to generate a positive rectangular wave pulse signal upon receiving the microcontroller edge trigger, wherein the timing of a rising edge and a falling edge of the positive rectangular wave pulse signal are controlled by adjustable delay elements;'}, {'b': 303', '302', '303', '302, 'ii. a buffer circuit (), operatively connected to the signal shaping circuit (), wherein the buffer circuit () is configured to convert the positive rectangular wave pulse signal of the signal shaping circuit () to a differential pulse,'}], 'c. a light emitting diode (LED) pulsing driver circuit (), operatively connected to an LED (), triggered by the microcontroller edge trigger, the LED pulsing driver circuit comprising{'b': 302', '303', '303', '303', '304, 'wherein when the microcontroller edge trigger is received, the signal shaping circuit () generates a ...
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