15-09-2022 дата публикации
Номер: US20220287328A1
The invention relates to a polypeptide for the hydrolytic cleavage of zearalenone and/or at least one zearalenone derivative, said polypeptide being a hydrolase having an amino acid sequence selected from the group consisting of SEQ ID NO: 1-15 or a functional variant thereof, wherein the sequence of the functional variant is at least 40% identical to at least one of the amino acid sequences. The invention also relates to: an additive containing the polypeptide; an isolated polynucleotide that encodes the polypeptide; and a method for the hydrolytic cleavage of zearalenone and/or of at least one zearalenone derivative using the polypeptide. 16-. (canceled)7. A method for hydrolytic cleavage of zearalenone or at least one zearalenone derivative selected from the group of α-ZEL ((2E ,7R ,11S)-7 ,15 ,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]-octadeca-1(18) ,2 ,14 ,16-tetraen-13-one) , β-ZEL (2E ,7S ,11S)-7 ,15 ,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(18) ,2 ,14 ,16-tetraen-13-one) , α-ZAL ((7R ,11S)-7 ,15 ,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(18) ,14 ,16-trien-13-one) , β-ZAL ((7S ,11S)-7 ,15 ,17-trihydroxy-11-methyl-12-oxabicyclo[12.4.0]octadeca-1(14) ,15 ,17-trien-13-one) , Z14G ((2E ,11S)-15-hydroxy-11-methyl-17-[(3R ,4S ,5S ,6R)-3 ,4 ,5-trihydroxy-6-(hydroxymethyl)-tetrahydropyran-2-yl] oxy-12-oxabicyclo[12.4.0]octadeca 1(18)2 ,14 ,16-tetraene-7 ,13-dione) , Z14S ([(2E ,11S)-15-hydroxy-11-methyl-7 ,13-dioxo-12-oxabicyclo[12.4.0]octadeca-1(18) ,2 ,14 ,16-tetraen-17-yl] hydrogen sulfate) and ZAN ((11S)-15 ,17-dihydroxy-11-methyl-12-oxabicyclo-[12.4.0]octadeca-1(18) ,14 ,16-triene-7 ,13-dione) , wherein the zearalenone or at least one zearalenone derivative is/are hydrolyzed by a polypeptide having an amino acid sequence selected from SEQ ID NO: 13 or a functional variant thereof , wherein the sequence identity between the functional variant and the amino acid sequence selected from SEQ ID NO: 13 is at least 70%; and wherein the ...
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