Sample Collection System And Method

An apparatus or method for removing water and concentrating an analyte in solution, wherein the concentrated analyte sample is delivered directly to a vial, such as an autosampler vial that is capable of use in a gas chromatography autosampler.

Sample processing device and method

A sample processing device is disclosed, which sample processing device comprises a first substrate and a second substrate, where the first substrate has a first surface comprising two area types, a first area type with a first contact angle with water and a second area type with a second contact angle with water, the first contact angle being smaller than the second contact angle. The first substrate defines an inlet system and a preparation system in areas of the first type which two areas are separated by a barrier system in an area of the second type. The inlet system is adapted to receive a sample liquid comprising the sample and the first preparation system is adapted to receive a receiving liquid. In a particular embodiment, a magnetic sample transport component, such as a permanent magnet or an electromagnet, is arranged to move magnetic beads in between the first and second substrates.

Microfluidic fluid separator and related methods

A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided.

Method for producing agglutinating reagent, agglutinating reagent or product produced thereby, and method for measuring analysis object using the same, and test kit and analysis device

Hemoglobin in a sample solution is quickly and reliably denatured; at the same time, quick and accurate measurement of hemoglobin and a hemoglobin derivative is realized. In a method for measuring hemoglobin and a hemoglobin derivative, and a reagent composition, a measurement kit, an analysis device, and an analysis system used in the method, a sample solution containing a blood component is treated with a nonionic surfactant, an oxidizing agent, and a metal salt to denature hemoglobin in the sample solution to measure the hemoglobin, and thereafter the amount of a hemoglobin derivative in the sample is measured by an immunological method using an antibody specifically binding to a denatured site of the denatured hemoglobin derivative.

Fluorescent detection of in vitro translated protein on a solid surface

Disclosed herein are methods and kits useful in the detection of protein folding and in the identification of compounds that promote proper protein folding. In one example approach, fluorophores and a protein tag are incorporated into a nascent polypeptide within a ribosome-nascent-chain complex during cell free translation and the resulting labeled ribosome-nascent-chain complex is conjugated to a solid surface via the tag. Fluorescence imaging via FRET is then preformed to assess the folding state of the ribosome-nascent-chain complex under a variety of conditions.

LIPOPROTEIN ANALYSIS BY DIFFERENTIAL CHARGED-PARTICLE MOBILITY

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention. 1. An apparatus for differential charged-particle mobility analysis , the apparatus comprising:one or more pumps adapted to transport sample through a capillary;an ionizer adapted to charge particles of the sample as the sample flows within the capillary; andan ion mobility analyzer adapted to perform a differential charged-particle mobility analysis on the sample of charged particles.2. The apparatus according to further comprising an autosampler adapted to provide a sample for differential charged-particle mobility analysis to the one or more pumps.3. The apparatus according to claim 1 , wherein the sample comprises lipoproteins.4. The apparatus according to claim 1 , wherein the one or more pumps comprise a high-flow pump adapted to provide the sample to a nanoflow pump claim 1 , the nanoflow pump adapted to provide the sample to the capillary.5. The apparatus according to claim 4 , wherein the high-flow pump pumps the sample at a rate of approximately 15-25 microliters per minute claim 4 , and wherein the nanoflow pump pumps the sample at a rate of approximately 100-200 nanoliters per minute.6. The apparatus according to claim 5 , wherein the ionizer comprises a conductive union around a part of the capillary.7. The apparatus according to claim 6 , wherein the conductive union applies a charge to ...

Light-controlled electrokinetic assembly of particles near surfaces

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

MULTIPLEX IMMUNOASSAYS FOR HEMOGLOBIN, HEMOGLOBIN VARIANTS, AND GLYCATED FORMS

Hemoglobin, its variants, and glycated forms of each are determined individually in a multiplex assay that permits correction of the measured level of HbA1c to account for glycated variants and other factors related to the inclusion of the variants in the sample. New antibodies that are particularly well adapted to the multiplex assay are also provided. 1. A monoclonal antibody that selectively binds to hemoglobin variant HbC and glycated HbC , wherein the antibody binds to HbC minimal epitope TPKEKSAVT(SEQ ID NO:1) and is raised against and HbC immunogen H2N-VHLTPKEKSAVTALW-C—CONH2 (SEQ ID NO:27).2. A monoclonal antibody that selectively binds to hemoglobin variant HbS and glycated HbS , wherein the antibody binds to HbS minimal epitope LTPVEKSAVT(SEQ ID NO:3) and is raised against an HbS immunogen H2N-VHLTPVEKSAVTALW-C—CONH2 (SEQ ID NO:26).3. An immunoassay reagent composition comprising a monoclonal antibody that selectively binds to hemoglobin variant HbC and glycated HbC , wherein the antibody binds to HbC minimal epitope TPKEKSAVT(SEQ ID NO:1) and is raised against and HbC immunogen H2N-VHLTPKEKSAVTALW-C—CONH(SEQ ID NO:27); or a monoclonal antibody that selectively binds to hemoglobin variant HbS and glycated HbS , wherein the antibody binds to HbS minimal epitope LTPVEKSAVT(SEQ ID NO:3) and is raised against an HbS immunogen H2N-VHLTPVEKSAVTALW-C—CONH(SEQ ID NO:26).4. The immunoassay reagent composition of claim 3 , comprising the monoclonal antibody that selectively binds to hemoglobin variant HbC and glycated HbC and the monoclonal antibody that selectively binds to hemoglobin variant HbS.5. The immunoassay composition of claim 3 , further comprising a monoclonal antibody that selectively binds to hemoglobin variant HbD and glycated HbD and is raised against an HbD immunogen HN-CYG-VLAHHFGKQFTPPVQAA-CONH(SEQ ID NO:32) or HN-QFTPPVQAAYQKVVAGV-GYC—CONH(SEQ ID NO:33); or a monoclonal antibody that selectively binds to hemoglobin variant HbE and glycated HbE ...

Tagged ligands for enrichment of rare analytes from a mixed sample

Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.

MASS SPECTROMETRIC DETERMINATION OF EICOSAPENTAENOIC ACID AND DOCOSAHEXAENOIC ACID

The invention relates to the detection of DHA and EPA. In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods. 1. A method for determining the amount of one or more omega-3 fatty acids in a sample by mass spectrometry , the method comprising:(i) ionizing said one or more omega-3 fatty acids from the sample to generate one or more ions detectable by mass spectrometry;(ii) determining the amount of said one or more omega-3 fatty acids ions by mass spectrometry; and(iii) relating the amount of DHA ions to the amount of one or more omega-3 fatty acids in the sample, wherein said one or more omega-3 fatty acids comprises docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA).2. The method of claim 1 , wherein said ionizing comprises atmospheric pressure chemical ionization (APCI).3. The method of claim 2 , wherein said APCI is in negative ionization mode.4. The method of claim 1 , wherein said ionizing comprises electrospray ionization (ESI).5. The method of claim 1 , wherein mass spectrometry comprises tandem mass spectrometry.6. The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 327.2±0.5.7. The method of claim 1 , wherein said one or more ions comprise an ion with a mass to charge ratio (m/z) of 301.2±0.5.8. The method of claim 1 , wherein said sample comprises human serum or plasma.9. The method of claim 1 , wherein said sample is subjected to a hydrolyzing agent prior to ionization.10. The method of claim 8 , wherein said hydrolyzing agent is an acid.11. The method of claim 1 , wherein said one or more omega-3 fatty acids is subjected to liquid/liquid extraction prior to ionization.12. The method of claim 1 , wherein said one or more omega-3 fatty acids is purified by liquid chromatography prior to ionization.13. The method of claim 12 , wherein said liquid chromatography comprises high performance liquid ...

BLOOD AND BIOLOGICAL SAMPLE COLLECTION DEVICE AND METHOD

Specially designed collection strips and their processing. By using specially designed collection strips, having a backer and one or more absorbent pads, in conjunction with a unique processing method, the processes of analyzing biological samples such as blood, or the like, may be done efficiency with the elimination of cross contamination risk. Identification of the sample stays with the sample throughout the process as it resides on the collection strip. The strip absorbs a known volume. The sample with identification is placed directly in an elution solution, without mechanically separating the sample from its identification information. Elimination of the need for mechanical separation tends to reduce cross contamination, as well as reducing sample processing time. 1. A blood collection device that does not require punching , or folding to obtain a sample for processing comprising:a flat rectangular elongated backer having a first end and a second end;a planar label area disposed on the first end upon which identifying information of a collected sample is recorded; anda separate absorbent pad attached at the second end of the flat rectangular elongate backer that does not require punching to obtain a sample whereby a blood sample is applied to the absorbent pad and dried for later testing, the construction of the blood collection device not requiring a folding, a punching out, or detachment of a portion of the blood collection device containing the blood sample, and whereby the identifying information of the collected sample stays affixed to the sample during processing of the sample.2. The blood collection device of claim 1 , in which the absorbent pad is cellulose.3. The blood collection device of claim 1 , in which the absorbent pad is polyester.4. The blood collection device of claim 1 , in which the absorbent pad is glass fiber.5. The blood collection device of claim 1 , in which the absorbent pad is treated with a detergent.6. The blood collection device ...

Methods and Devices for Using Mucolytic Agents Including N-Acetyl Cysteine (NAC)

Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer. 1. A method for detecting MxA in a sample on a sample analysis device that includes a flow of an elution medium from an elution medium application zone to a detection zone , comprising the steps of:a) transferring a blood sample that includes lymphocyte cells onto a sample application zone on the sample analysis device;b) applying an elution medium that includes a lysis agent capable of lysing lymphocyte cells to the elution medium application zone, such that the elution medium, including the at least one lysis agent, flows through the sample application zone toward the detection zone lysing lymphocyte cells, thereby releasing intracellular MxA present within the lymphocyte cells into the elution medium prior to reaching the detection zone; andc) detecting the presence of MxA at the detection zone on the sample analysis device.2. The method of claim 1 , wherein a conjugate zone is located between the sample application zone and the detection zone.3. The method of claim 1 , wherein the sample is whole blood.4. The method of claim 1 , further comprising the step of detecting a presence of an extracellular protein at the detection zone.5. The method of claim 1 , wherein the device is a lateral flow immunoassay device.6. The method of claim 1 , wherein the device is a chromatography test strip.7. The method of claim 1 , further comprising a blocking zone comprising a ...

Method for Profiling Phytohormone Levels in Plant Tissue

The present invention provides a method for profiling phytohormone levels in plant tissue or tissue of other plastid containing organisms, i.e. a method for the simultaneous determination of a multitude of phytohormone levels in plant tissue or tissue of other plastid containing organisms, which method comprises the following steps: i. extraction of particulate tissue material of the plastid containing organism, in particulate tissue material of plants, with a liquid extractant, which is a mixture of at least one water miscible neutral organic solvent having from 1 to 3 carbon atoms and 1 heteroatom selected from O and N with water containing from 0.1 to 5% by weight, based on the extractant, of at least one acid, whereby a first liquid extract is obtained; ii. contacting the liquid extract obtained in step i. with a solid absorbent having a hydrophobically modified surface and removing the solid absorbent to obtain a second liquid extract; iii. evaporating the solvent from the second extract and then re-dissolving the obtained residue in a solvent mixture of at least one water miscible neutral organic solvent having from 1 to 3 carbon atoms and 1 heteroatom selected from O and N with water containing from 0.1 to 5% by weight, based on the solvent mixture, of at least one acid to obtain a reconstituted extract; iv. purification of the reconstituted extract by contacting it with a solid absorbent having a hydrophobically modified surface to obtain a purified reconstituted extract (eluate); and v. determining the relative concentrations of at least two, frequently at least 4, in particular at least 6, especially at least 8 or at least 10 phyto, e.g. from 2 to 60, frequently from 4 to 50, in particular from 6 to 45, especially from 8 to 40 or from 10 to 30 plant hormones (phytohormones) in the purified reconstituted extract obtained in step iv. by directly subjecting the purified reconstituted extract to an analyzing unit comprising a separation unit for separation the ...

Monoclonal antibodies with specificity for fetal erythroid cells

The present invention concerns a monoclonal antibody and corresponding hybridoma cells and antigens suitable for isolating fetal cells from maternal blood. The inventive monoclonal antibody reacts with a surface antigen present on fetal red blood cells including their nucleated precursor cells, but not with surface antigens on adult erythroid cell.

Compositions and methods for the detection of antibodies to native human leukocyte antigen

Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.

Method and apparatus for automated analysis

An apparatus for pretreatment of a sample of whole blood in a discrete fluid analyzing instrument comprises automated means for handling and analyzing the sample and means for performing a pretreatment step on the sample or a sub-sample of the sample. The means for pretreatment are used for immobilizing at least one substance or analyte from the sample or sub-sample wherein the substance or analyte is reversibly immobilized. Usually, the apparatus further comprises means for eluting the substance or analyte from the capture means prior to analysis. 1. An apparatus for automated pretreatment of a sample of whole blood in a discrete fluid analyzing instrument , said apparatus comprising:a hemolyzer configured to hemolyze at least a subsample of the whole blood,a pretreatment unit configured to retain at least one substance or analyte from the portion of whole blood hemolyzed by the hemolyzer, and 'wherein the apparatus is configured to operate the hemolyzer, pretreatment unit and dispenser such that hemolyzation, pretreatment and elution are performed in an automated fashion.', 'a dispenser configured to elute the at least one substance or analyte retained by the pretreatment unit,'}2. The apparatus according to wherein the pretreatment unit comprises capture means configured to retain the at least one substance or analyte.3. The apparatus according to wherein the pretreatment unit is configured to reversibly remove the at least one substance or analyte.4. The apparatus according to wherein the pretreatment unit is configured to reversibly remove the at least one substance or analyte.5. The apparatus according to further comprising a measurement unit configured to measure at least one property of the hemolyzed portion of whole blood retained by the pretreatment unit.6. The apparatus according to wherein the apparatus is configured to simultaneously or consecutively direct non-pretreated samples or non-pretreated subsamples to the measurement unit.7. The apparatus ...

COMPOSITIONS AND PROCESSES FOR IMPROVED MASS SPECTROMETRY ANALYSIS

The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry. 1. (canceled)2. A method of preparing a substrate suitable for use in Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry , comprising depositing the matrix material on the substrate , wherein:the matrix material is deposited on the substrate in an array of spots; andthe adduct-reducing additive comprises ascorbic acid, or a salt, tautomer or analog thereof, in an amount that reduces the amount of an adduct formed under mass spectrometry conditions.3. The method of claim 2 , wherein the substrate comprises silica.4. The method of claim 2 , wherein the matrix comprises one or more of 3-hydroxypicolinic acid claim 2 , di-ammoniumcitrate claim 2 , 2 claim 2 ,5-dihydroxybenzoic acid claim 2 , Alpha-cyano-4-hydroxycinnamic acid claim 2 , 2 claim 2 ,4 claim 2 ,6-trihydroxyacetophenone claim 2 , T-2-(3-(4-t-Butyl-phenyl)-2-methyl-2-propenylidene)malononitrile claim 2 , dithranol claim 2 , sinapic acid claim 2 , trans-3-indoleacrylic acid claim 2 , or 2-(4-hydroxyphenylazo)benzoic acid.6. The method of claim 2 , wherein the matrix comprises 3-hydroxypicolinic acid.7. The method of claim 2 , wherein the matrix comprises di-ammonium citrate.8. The method of claim 2 , further comprising ammonium oxalate.9. The method of claim 2 , wherein the additive consists essentially of ascorbic acid claim 2 , or a salt claim 2 , tautomer or analog thereof.10. The method of claim 9 , wherein the additive consists of ascorbic acid claim 9 , or a salt claim 9 , tautomer or analog thereof.11. The method of claim 9 , wherein the additive consists essentially of ascorbic acid.12. The method of claim 11 , wherein ...

LIPOPROTEIN ANALYSIS BY DIFFERENTIAL CHARGED-PARTICLE MOBILITY

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention. 1. A method for purifying lipoproteins for ion mobility analysis , the method comprising:admixing a solution comprising lipoproteins and non-lipoproteins with one or more lipoprotein-capture ligands capable of binding lipoproteins to form a lipoprotein/lipoprotein-capture ligand complex;isolating the lipoprotein/lipoprotein-capture ligand complex; andreleasing the lipoproteins from the lipoprotein/lipoprotein-capture ligand complex and subjecting the lipoproteins to ion mobility;wherein the method does not include centrifugation.2. The method of claim 1 , wherein the lipoproteins are selected from the group consisting of HDL claim 1 , LDL claim 1 , Lp(a) claim 1 , IDL and VLDL.3. The method of claim 1 , wherein the lipoprotein-capture ligand is selected from the group consisting of an aptamer and an antibody.4. The method of claim 1 , wherein the aptamer or antibody comprises a biotinylated aptamer or a biontinylated antibody.5. The method of claim 1 , wherein the lipoprotein-capture ligand is an aptamer.6. The method of claim 5 , wherein the aptamer comprises RNA or DNA.7. The method of claim 5 , wherein the aptamer binds Apo A1 claim 5 , Apo B claim 5 , or Apo(a).8. The method of claim 1 , wherein the lipoprotein-capture ligand is an antibody.9. The method of wherein the antibody complexes the ...

AUTOMATIC ANALYZER AND METHOD FOR WASHING SAMPLE-PIPETTING PROBE

When the type is to be changed from serum (preceding sample) to urine (current sample), “serum” is set to a preceding type and “urine” is set to a measurement type at number 1 in a condition number. At condition number 1, the wash type is pattern 1, with washing performed once with detergent 1. Where the preceding sample is serum and the current sample is CSF, the condition number is 2 and the wash type is pattern 2, with washing performed twice using detergent 1 and once with detergent 2. Where the preceding sample is urine and the current sample is CSF, the condition number is 3 and the wash type is pattern 3, with washing performed once with detergent 1, once with detergent 2, and once with water. In the case of pattern 4, washing is performed three times with detergent 1. 1. An automatic analyzer for analyzing samples of a plurality of types , the automatic analyzer comprising:a reaction disk for holding a plural of reaction vessels;a sample-pipetting probe for pipetting a sample to be measured into the reaction vessel;a rinse bath in which the sample-pipetting probe is washed;a display unit for displaying a setup screen for previously setting a washing method having wash fluid types for washing the sample-pipetting probe on a basis of a combination of a type of a first sample that has been pipetted and a type of a second sample to be pipetted consecutively next in case that there exists the combination affecting to an analysis of a sample with regard to a relationship between the type of the first sample and the type of the second sample;an information storing memory for storing information set by the setup screen and a type of a measurement sample requested to analyze the sample; anda controller for determining a method of washing the sample-pipetting probe on a basis of the information stored in the information storing memory, the controller causing a washing operation of the sample-pipetting probe by use of the rinse bath,wherein the controller comprises:a ...

DIAGNOSTIC TEST SYSTEM USING MEASUREMENT OBTAINED FROM REFERENCE FEATURE TO MODIFY OPERATIONAL PARAMETER OF READER

A diagnostic test system includes a housing, a reader, and a data analyzer. The housing includes a port constructed and arranged to receive a test strip that includes a flow path for a fluid sample, a sample receiving zone couple to the flow path, a label that specifically binds a target analyte, a detection zone coupled to the flow path and comprising a test region exposed for optical inspection and having an immobilized test reagent that specifically binds the target analyte, and at least one reference feature. The reader is operable to obtain light intensity measurements from exposed regions of the test strip when the test strip is loaded in the port. The data analyzer is operable to perform operations including at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature. 130-. (canceled)31. A diagnostic test system , comprising:a housing comprising a port constructed and arranged to receive a test strip that comprises a flow path for a fluid sample, a sample receiving zone coupled to the flow path, a label that specifically binds a target analyte, a detection zone coupled to the flow path and comprising a test region exposed for optical inspection and having an immobilized test reagent that specifically binds the target analyte, and at least one reference feature;a reader operable to obtain light intensity measurements from exposed regions of the test strip when the test strip is loaded in the port; anda data analyzer operable to perform operations comprising at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a ...

HIGH SENSITIVITY QUANTITATION OF PEPTIDES BY MASS SPECTROMETRY

The instant invention provides an economical flow-through method for determining amount of target proteins in a sample. An antibody preparation (whether polyclonal or monoclonal, or any equivalent specific binding agent) is used to capture and thus enrich a specific monitor peptide (a specific peptide fragment of a protein to be quantitated in a proteolytic digest of a complex protein sample) and an internal standard peptide (the same chemical structure but including stable isotope labels). Upon elution into a suitable mass spectrometer, the natural (sample derived) and internal standard (isotope labeled) peptides are quantitated, and their measured abundance ratio used to calculate the abundance of the monitor peptide, and its parent protein, in the initial sample. 1. A method for quantifying the amount of at least on target protein in a sample comprising the steps of:1. digesting the sample to provide peptides,2. preparing at least one labeled monitor peptide comprising a subsequence of said target protein(s) to provide an internal standard,3. adding an aliquot of the product of step 2 containing a known amount of labeled synthetic peptide to the product of step 1,4. loading the product of step 3 onto at least one support which has attached thereto at least one binding agent which binds to at least one monitor peptide,5. washing the support(s) of step 4 to remove unbound peptides,6. eluting the monitor peptide (s) from the binding agent(s) bound to the support (s),7. subjecting at least one of the monitor peptides present in the eluate(s) obtained in step 6 to mass spectrometry to determine the amount of monitor peptide(s) in the digest by comparison with the labeled synthetic standard(s).2. The method of wherein the label is a stable isotope or mixture thereof3. The method of wherein the isotope is N.4. The method of wherein the isotope is C.5. The method of wherein the isotope is O.6. The method of wherein claim 1 , in step 4 claim 1 , the binding agent is an ...

LIPOPROTEIN ANALYSIS BY DIFFERENTIAL CHARGED-PARTICLE MOBILITY

The invention provides methods of preparation of lipoproteins from a biological sample, including HDL, LDL, Lp(a), IDL, and VLDL, for diagnostic purposes utilizing differential charged particle mobility analysis methods. Further provided are methods for analyzing the size distribution of lipoproteins by differential charged particle mobility, which lipoproteins are prepared by methods of the invention. Further provided are methods for assessing lipid-related health risk, cardiovascular condition, risk of cardiovascular disease, and responsiveness to a therapeutic intervention, which methods utilize lipoprotein size distributions determined by methods of the invention. 1. A system for analyzing size distribution of lipoproteins , the system comprising a processor and memory having machine instructions stored thereon , the instructions being executable by the processor to cause the processor to:collect, via an ion mobility analyzer configured to receive samples for differential charged-particle mobility analysis, differential ion mobility data for a sample having one or more lipoproteins;determine, using the differential ion mobility data, a lipoprotein particle size distribution for one or more lipoproteins, wherein determining the lipoprotein particle size distribution comprises determining a best fit for a set of one or more regions of interest for lipoprotein particle sizes;generate a visual representation of the lipoprotein particle size distribution; andoutput the visual representation via a display or a printer.2. The system of claim 1 , wherein the instructions are executable to cause the processor to generate a graphical representation of the lipoprotein particle size distribution as the visual representation.3. The system of claim 2 , wherein the instructions are further executable to cause the processor to determine a curve corresponding to the best fit claim 2 , and include the curve in the visual representation.4. The system of claim 1 , wherein the ...

Method for preparing a sample for chromatographic separation processes and system for carrying out a sample preparation

A method for preparing a sample for chromatographic separation processes, in which a sample vessel is partially filled with a substance to be examined and is closed, is described. The substance to be examined is subjected to a thermo-chemical reaction in which at least one sample component is converted into another substance, and in which by use of a removing device samples are removed from the sample vessel for analytical examination. Also, the sample vessel forms a cavity, into which the substance to be examined is introduced as a core and a heating section for indirect heat transfer is applied along the filling of substance to be examined.

METHODS AND DEVICES FOR USING MUCOLYTIC AGENTS INCLUDING N-ACETYL CYSTEINE (NAC)

Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer. 1. A method for detecting MxA in a sample on a sample analysis device that includes a flow of an elution medium from an elution medium application zone to a detection zone , comprising the steps of:a) transferring a blood sample that includes lymphocyte cells onto a sample application zone on the sample analysis device;b) applying an elution medium that includes a lysis agent capable of lysing lymphocyte cells to the elution medium application zone, such that the elution medium, including the at least one lysis agent, flows through the sample application zone toward the detection zone lysing lymphocyte cells, thereby releasing intracellular MxA present within the lymphocyte cells into the elution medium prior to reaching the detection zone; andc) detecting the presence of MxA at the detection zone on the sample analysis device.2. The method of claim 1 , wherein a conjugate zone is located between the sample application zone and the detection zone.3. The method of claim 1 , wherein the sample is whole blood.4. The method of claim 1 , further comprising the step of detecting a presence of an extracellular protein at the detection zone.5. The method of claim 1 , wherein the device is a lateral flow immunoassay device.6. The method of claim 1 , wherein the device is a chromatography test strip.7. The method of claim 1 , further comprising a blocking zone comprising a ...

Systems and methods for preparing samples for chemical analysis using a cooled digestion zone

An apparatus for preparing samples for chemical analysis includes a container receptacle for receiving a sample container having a crucible portion and an expansion portion. The container receptacle includes a heating compartment and a cooling compartment spaced apart from the heating compartment. The heating compartment is shaped to receive the crucible portion of the sample container, and the cooling compartment is shaped to receive the expansion portion of the sample container. The apparatus also includes a heating mechanism for heating the sample within the crucible portion of the sample container, a first cooling mechanism for cooling the expansion portion of the sample container, and a second cooling mechanism for cooling the crucible portion of the sample container.

Quantitation of tamoxifen and metabolites thereof by mass spectrometry

Provided are methods for determining the amount of tamoxifen and its metabolites in a sample by mass spectrometry. In some aspects, the methods provided herein comprise determining the amount of norendoxifen. In some aspects, the methods provided herein comprise determining the amount of norendoxifen and tamoxifen. In some aspects, the methods provided herein comprise determining the amount of norendoxifen and other tamoxifen metabolites. In some aspects, the methods provided herein comprise determining the amount of tamoxifen, norendoxifen, and other tamoxifen metabolites.

Methods and Devices for Using Mucolytic Agents Including N-Acetyl Cysteine (NAC)

Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer.

INDEPENDANT HEATING OF SAMPLES IN A SAMPLE HOLDER

There is described a method for heating a sample material in a sample holder, the method comprising receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein; dynamically forming an individual mini microwave cavity around the sample recipient; and applying microwaves generated by at least one microwave generator directly to the sample. 1. A method for heating a sample material in a sample holder , the method comprising:receiving the sample holder in a heating chamber of a heating system, the sample holder having at least one sample recipient with the sample material therein;dynamically forming an individual mini microwave cavity around the at least one sample recipient inside the heating chamber; andapplying microwaves generated by at least one microwave generator directly to the sample in the at least one sample recipient within the mini microwave cavity.2. The method of claim 1 , wherein dynamically forming the mini microwave cavities comprises mating a set of first partial mini cavities with a set of complementary second partial mini cavities.3. The method of claim 2 , wherein the set of first partial mini cavities are in the heating chamber and the set of complementary second partial mini cavities are on the sample holder.4. The method of claim 1 , further comprising mechanically positioning the sample holder in the heating chamber to align the set of first partial mini cavities with the set of complementary second partial mini cavities.5. The method of claim 1 , wherein dynamically forming an individual mini microwave cavity comprises forming a plurality of individual mini microwave cavities around each one of a plurality of sample recipients in the sample holder.6. The method of claim 5 , wherein applying microwaves comprises applying microwaves generated using at least two separate microwave generators.7. The method of claim 6 , wherein applying microwaves ...

Capillary hematocrit separation structure and method

A capillary hematocrit separation structure is included within a housing having a fluid inlet port, a reaction region, and a capillary pathway connecting the inlet port and the reaction region. The capillary pathway is dimensioned so that the driving force for the movement of liquid through the capillary pathway arises from capillary pressure. A plurality of obstructions are fixed in the capillary pathway, each obstruction having a concave portion facing toward the vented reaction region on the down stream side of the obstructions as viewed with reference to a liquid flowing from the inlet port to the reaction region. The capillary pathway in a hematocrit separation structure for a single drop sample size includes about 10<5> obstructions, each obstruction including a concave portion having a volume of between about 10<-4> to 10<-5> mu l for selectively receiving hematocrit. <IMAGE>

Protein separation via multidimensional electrophoresis

The present method provides methods and apparatus for separating proteins using a series of electrophoretic methods that utilize controlled fractionation and labeling techniques to resolve mixtures of proteins. The samples for each electrophoretic method other than the initial method, contain only a subset of proteins resolved in the preceding method. The methods can be used in a variety of different applications including, creating proteomic databases, comparative expression studies, diagnostics, structure activity relationships and metabolic engineering investigations.

Protein separation by electrophoresis

The present method provides methods and apparatus for separating proteins using a series of electrophoretic methods that utilize controlled fractionation and labeling techniques to resolve mixtures of proteins. The samples for each electrophoretic method other than the initial method, contain only a subset of proteins resolved in the preceding method. The methods can be used in a variety of different applications including, creating proteomic databases, comparative expression studies, diagnostics, structure activity relationships and metabolic engineering investigations.

Diagnostic test element with multilayered test field and metod to assay analytes using it

Diagnostic support system (I) containing a reagent system held on the support comprises a film on one side for observation or measurement of the response and one on the other for administering the test solution .

Analysis of circulating tumor cells, fragments, and debris

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

Tagged ligands for enrichment of rare analytes from a mixed sample

Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.

Analysis of Circulating Tumor Cells, Fragments, and Debris

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

Analysis of circulating tumor cells, fragments, and debris

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CELLSPOTTER® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

Swab-based diagnostic systems

A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.

Method of making a microbead array with attached biomolecules

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Biological diagnostic device and method of use

A biological diagnostic device and process for efficiently and accurately analyzing a sample of a biological fluid for in analyte of interest. The device, which is a preferred embodiment utilized for the analysis of whole blood samples, comprises a diagnostic test element and a sample application unit comprising a fluid delivery element and means for providing sample fluid to the fluid delivery element. The fluid delivery element comprises a layer having a plurality of grooves, or channels, in the surface thereof which is adjacent the test element. The fluid which is provided to the grooves is subsequently delivered to the test element.

Method of making a microbead array with attached biomolecules

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Swab-based diagnostic systems

A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.

Swab-Based Diagnostic Systems

A diagnostic test system for detecting the presence or absence of an analyte within a test sample is provided. For instance, the system may include a swab and a detection unit. The detection unit includes a first component that is capable of receiving the swab, the first component defining an insertion chamber within which a fluid is capable of being retained. The detection unit also includes a second component that defines a detection chamber within which an assay for detecting the presence or absence of the analyte is capable of being contained. The first component is rotatable relative to the second component from an inactive position to an active position. In the inactive position, the fluid remains substantially contained within the insertion chamber. In the active position, the fluid may flow from the insertion chamber to the detection chamber and contact the assay.

Methods and compositions related to determination and use of white blood cell counts

Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Retentate chromatography and protein chip arrays with applications in biology and medicine

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Analysis of circulating tumor cells, fragments, and debris

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

Method and apparatus for automated analysis

A method and apparatus for pretreatment of a sample of whole blood in a discrete fluid analyzing instrument comprises automated means for handling and analyzing the sample and means for performing a pretreatment step on the sample or a sub-sample of the sample. The means for pretreatment are used for immobilizing at least one substance or analyte from the sample or sub-sample wherein the substance or analyte is reversibly immobilized. Usually, the apparatus further comprises means for eluting the substance or analyte from the capture means prior to analysis.

Method using an apparatus for separation of biological fluids

A cell containing sample is separated into a cell containing portion and a substantially cell depleted portion, by mixing the sample with particles to produce a cell containing network, and separating the network from the remaining substantially cell depleted portion within a plurality of confining walls, wherein at least one of the walls is flexible. While in some aspects the separation is performed employing a magnetic force, in other aspects the separation is performed using two forces, wherein one force is a magnetic force and the other force is a mechanical force. It is contemplated that whole blood may be used as the sample, and that the cell-containing portion largely comprises a network of inter-linked red blood cells. It is especially contemplated that separation involves antiligands, preferably primary antibodies that bind to a ligand such as antigen on or in red blood cell membranes, and secondary antibodies that bind to the primary antibodies. It is also contemplated that the primary antibodies can be coupled to the surfaces of paramagnetic beads.

Retentate chromatography and protein chip arrays with applications in biology and medicine

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Retentate chromatography and protein chip arrays with applications in biology and medicine

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Retentate chromatography and protein chip arrays with applications in biology and medicine

This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.

Method and apparatus for automated analysis

A method for pretreatment of a sample of whole blood in a discrete fluid analyzing instrument comprises automated means for handling and analyzing the sample and means for performing a pretreatment step on the sample or a sub-sample of the sample. The means for pretreatment are used for immobilizing at least one substance or analyte from the sample or sub-sample wherein the substance or analyte is reversibly immobilized. Usually, the apparatus further comprises means for eluting the substance or analyte from the capture means prior to analysis.

Apparatus for automated analysis

An apparatus for pretreatment of a sample of whole blood in a discrete fluid analyzing instrument comprises automated means for handling and analyzing the sample and means for performing a pretreatment step on the sample or a sub-sample of the sample. The means for pretreatment are used for immobilizing at least one substance or analyte from the sample or sub-sample wherein the substance or analyte is reversibly immobilized. Usually, the apparatus further comprises means for eluting the substance or analyte from the capture means prior to analysis.

Method for rapid base sequencing in DNA and RNA

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

Microfluidic-based electrospray source for analytical devices

This invention provides microfluidic devices and methods for using the same. Microfluidic devices of the present invention comprises a first elastic layer, a fluid flow channel within the elastic layer; and a means for providing a fluid sample from the fluid flow channel to an analytical device. The present invention also provides an analytical apparatus comprising such a microfluidic device and an analytical device.

Microfluidic device based sample injection system for analytical devices

This invention provides a microfluidic sample injection apparatus (14, 100) for injecting a fluid sample into an analytical device (120) and a method for using the same. The microfluidic sample injection apparatus (14, 100) comprises a microfluidic device (100) and an integrated sample injection capillary (14) which is in fluid communication with a fluid flow channel of the microfluidic device (100).

Active programmable electronic devices for molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micromachining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific microlocations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Methods of registering trans-membrane electric potentials

Sensitive methods for identifying compounds having biological activity comprising combining living cells with two fluorescent membrane permeable ionic dyes having the same charge sign, the first of which has an emission spectrum which overlaps the excitation spectrum of the second fluorescent membrane penetrative dye. The fluorescence is then induced by illuminating the dyes at a wavelength corresponding to the excitation spectrum of the first fluorescent dye and emission is then registered at a wavelength corresponding to the emission spectrum of the second fluorescent dye (FRET). The change in the FRET is indicative of a modulation of cell membrane potential by the biologically active compounds.

Reticulocyte analyzing method and apparatus utilizing light scatter techniques

A reticulocyte analyzing method and apparatus in which a biological sample stream of ghosted red cells is passed into and through a point focused beam of light, such as laser light. A light detector structure is positioned with respect to the axis of the light beam to provide a light output pulse indicative of the passage of each cell. Electrically conductive contacts within the fluid stream can provide additional electrical pulse outputs of each cell. A staining reagent can be utilized with a ghosting reagent to further differentiate the reticulocytes. The light and the light and electronic produced output pulses or signals can be combined to define and quantify reticulocytes as distinct from other known cell types.

Methods for transport in molecular biological analysis and diagnostics

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridizations, antibody/antigen reactions, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Method for the electronic analysis of a sample oligonucleotide sequence

A self-addressable, self-assembling microelectronic device is designed and fabricated to actively carry out and control multi-step and multiplex molecular biological reactions in microscopic formats. These reactions include nucleic acid hybridization, antibody/antigen reaction, diagnostics, and biopolymer synthesis. The device can be fabricated using both microlithographic and micro-machining techniques. The device can electronically control the transport and attachment of specific binding entities to specific micro-locations. The specific binding entities include molecular biological molecules such as nucleic acids and polypeptides. The device can subsequently control the transport and reaction of analytes or reactants at the addressed specific micro-locations. The device is able to concentrate analytes and reactants, remove non-specifically bound molecules, provide stringency control for DNA hybridization reactions, and improve the detection of analytes. The device can be electronically replicated.

Self-addressable self-assembling microelectronic systems and devices for molecular biological analysis and diagnostics

A method for analyzing nucleic acid obtained from a cell sample on a platform is described. A platform having a cell selector, a nucleic acid selector, and an array of microlocations, wherein at least one microlocation has an associated capture sequence, is provided. The cell selector is contacted with a cell sample, wherein a portion of the cells remain associated with the cell selector. At least a portion of cells associated with the cell selector are lysed to release a nucleic acid sample. The nucleic acid selector is then contacted with the nucleic acid sample, such that a portion of the nucleic acid sample remains associated with the nucleic acid selector. The associated nucleic acid sample is then released from the nucleic acid selector and then is contacted with the array of microlocations, such that at least a portion of the released nucleic acid sample hybridizes with the capture sequence.

Method and apparatus for quantification of high density lipoprotein cholesterol

Determination of analytes in biological fluids in the presence of substances interfering with assays therefor

A method is provided, in one embodiment, for the determination of an analyte in a biological fluid sample in the presence of a substance interfering with an assay for the analyte. This embodiment is implemented by using antibodies to cause the selective immunoreaction of at least one of the analyte or the interfering substance and then conducting an assay for the analyte in at least one of the immunoreactants or the non-reactants. Another embodiment provides a disposable reaction device to implement the method. The invention is applicable to the detection of a wide variety of analytes, including cholesterol in a targeted lipoprotein class in the presence of cholesterol in another class; to targeted isozymes of enzymes such as creatine kinase, lactate dehydrogenase, amylase, and alkaline or acid phosphatases in the presence of other isozymes; as well as to targeted immunoglobulins in the presence of non-targeted immunoglobulins.

Amelioration of heterophile antibody immunosensor interference

The invention is directed to methods and devices for reducing interference from heterophile antibodies in an analyte immunoassay. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with non-human IgM or fragments thereof by dissolving into said sample a dry reagent to yield a non-human IgM concentration of at least about 20 μg/mL or equivalent fragment concentration; and (b) performing an electrochemical immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG or fragments thereof in addition to the IgM of fragments thereof.

Reducing leukocyte interference in competitive immunoassays

The invention is directed to methods and devices for reducing interference from leukocytes in competitive analyte immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads opsonized for leukocytes; and (b) performing a competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

Reducing leukocyte interference in non-competitive immunoassays

The invention is directed to methods and devices for reducing interference from leukocytes in an analyte immunoassay, and in particular in non-competitive immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads; and (b) performing a non-competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

Magnetic beads for reducing leukocyte interference in immunoassays

Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample with magnetic sacrificial beads opsonized to leukocytes, binding leukocytes in the sample to the magnetic sacrificial beads, and magnetically retaining the beads out of contact from an immunosensor.

Amelioration of heterophile antibody immunosensor interference

The invention is directed to methods and devices for reducing interference from heterophile antibodies in an analyte immunoassay. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with non-human IgM or fragments thereof by dissolving into said sample a dry reagent to yield a non-human IgM concentration of at least about 20 μg/mL or equivalent fragment concentration; and (b) performing an electrochemical immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG or fragments thereof in addition to the IgM of fragments thereof.

Reducing leukocyte interference in non-competitive immunoassays

The invention is directed to methods and devices for reducing interference from leukocytes in an analyte immunoassay, and in particular in non-competitive immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads; and (b) performing a non-competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

Reducing leukocyte interference in competitive immunoassays

The invention is directed to methods and devices for reducing interference from leukocytes in an competitive analyte immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads opsonized for leukocytes; and (b) performing a competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.

Magnetic beads for reducing leukocyte interference in immunoassays

Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample with magnetic sacrificial beads opsonized to leukocytes, binding leukocytes in the sample to the magnetic sacrificial beads, and magnetically retaining the beads out of contact from an immuno sensor.

Magnetic beads for reducing leukocyte interference in immunoassays

Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample with magnetic sacrificial beads opsonized to leukocytes, binding leukocytes in the sample to the magnetic sacrificial beads, and magnetically retaining the beads out of contact from an immunosensor.

Apparatus for determination of HDL cholesterol

The invention relates to an apparatus and a method useful in separating non-high density lipoproteins, referred to as "non-HDLs", from biological fluids containing them. A porous carrier is provided which contains a non-HDL precipitating agent. One need only contact the sample of interest to the carrier for one minute or less, after which the precipitated non-HDLs are no longer present in the sample being tested. The applications of the method include the ability to determine high density lipoproteins in the sample without interference from non-HDLs.

Manifold assembly

A manifold assembly that directs fluid to and from individually selectable sample receiving trays of a tissue processing system includes a manifold, fluid conduits machined in the manifold, and valves that may be selectively configured to provide a direct fluid path to or from a particular tray. The valves are controlled by a controller that positions each valve such that a desired path is created. Pressure is created in supply and/or drain bottles to supply or drain fluid from the trays supported by the manifold assembly. Independently operated heaters are provided on the manifold assembly to heat the trays to desired temperatures. Thermoelectric cooling elements may also provided to cool the heaters and/or trays.

Manifold assembly

A manifold assembly that directs fluid to and from individually selectable sample receiving trays of a tissue processing system includes a manifold, fluid conduits machined in the manifold, and valves that may be selectively configured to provide a direct fluid path to or from a particular tray. The valves are controlled by a controller that positions each valve such that a desired path is created. Pressure is created in supply and/or drain bottles to supply or drain fluid from the trays supported by the manifold assembly. Independently operated heaters are provided on the manifold assembly to heat the trays to desired temperatures. Thermoelectric cooling elements may also provided to cool the heaters and/or trays.

Methods and compositions related to determination and use of white blood cell counts

Described herein are methods and compositions for analyte detection with a portable instrument suitable for point-of-care analyses wherein the device may be used for a differential assay of blood components, such as lymphocyte populations or other cell populations, which can be used in diagnosis, and for monitoring treatment of diseases, such as HIV infection. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular-based analyses in conjunction with microsieve-based analysis cartridges.

Purification of silicon compounds

在竞争性免疫测定中减少白细胞干扰

本发明涉及用于在竞争性分析物免疫测定中减少来自白细胞的干扰的方法和设备。在一个实施方案中，本发明是包括如下步骤的方法：(a)用针对白细胞进行了调理作用的消耗珠子修正生物样品例如全血样品；和(b)对修正的样品进行竞争性免疫测定以测定所述分析物在所述样品中的浓度。优选，用IgG-涂覆的消耗珠子修正样品。

ＣａＣＯ↓３及びＣａＳＯ↓３の濃度の測定方法

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

Retentate chromatography and protein chip arrays with applications in biology and medicine

본 발명은 시료 중의 피분석물을 분할하기 위한 보유물 크로마토그래피 방법을 제공한다. 이 방법은 복수의 상이한 선택성 조건하에 피분석물을 기재에 흡착시키는 단계, 이 기재 상에 보유된 피분석물을 탈착 분광분석으로 검출하는 단계를 포함한다. 이 방법은 임상 진단과 약물 개발을 비롯한 의학 및 생물학에 유용하다. The present invention provides a retent chromatography method for partitioning analytes in a sample. The method includes adsorbing analyte to a substrate under a plurality of different selectivity conditions, and detecting analyte retained on the substrate by desorption spectroscopy. This method is useful for medicine and biology, including clinical diagnosis and drug development.

用于分析血液样品的方法和系统

公开了用于分析血液样品的方法、系统、以及计算机程序产品。一个实施方式是对血液样品中难以血影化细胞进行检测并计数的方法。另一个实施方式是分析血液样品中网织红细胞的方法。还提供了使用血细胞计数参数的方法。

用于分析血液样品的方法和系统

公开了用于分析血液样品的方法、系统、以及计算机程序产品。一个实施方式是对血液样品中难以血影化细胞进行检测并计数的方法。另一个实施方式是分析血液样品中网织红细胞的方法。还提供了使用血细胞计数参数的方法。

膜における分析物の光学的定量

(57)【要約】 膜に清澄剤を適用する工程を含む、膜における分析物の量を測定する方法が開示される。清澄剤は、膜とほぼ同じ屈折率を有する薬品であることができ、あるいは、清澄剤は、膜を溶解する溶解剤であることができる。分析物は、検出を容易にするように標識することができる。代表的な標識には、蛍光標識および検出可能粒子が含まれる。

Method and device for determining parameters of system in order to reduce corrosion in installation of primary oil processing

FIELD: measurement technology. SUBSTANCE: invention relates to determination of number of different substances in a liquid sample. Device contains at least one optical analysis method, not depending on volume and/or concentration, for determination of one of following properties: hydrogen potential pH, amount of chloride and/or amount of iron in sample. Optical property may be examined, fluorescent, or both, wherein it can be a result of adding into sample dyes, complexing agents, compounds, causing turbidity, and other reagents, causing optical effect. Method also involves use of BDD electrode for oxidation of substances (such as sulphoxide compounds), which otherwise would hinder optical analysis, and/or for washing sample gas. EFFECT: measurements can be carried out continuously, quickly, thereby avoiding inconvenience to beginning and termination of process, and device can be reused with such samples of very hard water, as water of settler of refinery. 15 cl, 11 dwg РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 594 659 C2 (51) МПК G01N 21/77 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ (21)(22) Заявка: ИЗОБРЕТЕНИЯ К ПАТЕНТУ 2013124969/28, 26.04.2012 (24) Дата начала отсчета срока действия патента: 26.04.2012 Приоритет(ы): (30) Конвенционный приоритет: (72) Автор(ы): ЦИОТА Стивен Р. (US), ВЕЛЦ Сасча (US), БАНКС Родни Х. (US) 27.04.2011 US 13/095,042 (43) Дата публикации заявки: 10.04.2015 Бюл. № 10 R U (73) Патентообладатель(и): НАЛКО КОМПАНИ (US) (45) Опубликовано: 20.08.2016 Бюл. № 23 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 02.10.2013 2 5 9 4 6 5 9 (56) Список документов, цитированных в отчете о поиске: EP 0469773 A2, 05.02.1992. US 2009/ 0177143 A1, 09.07.2009. US 5094958 A, 10.03.1992. US 5462880 A, 31.10.1995. US 2005/0009194 A1, 13.01.2005. US 3992109 A, 16.11.1976. RU 2300771 C1, 10.06.2007. RU 2011968 C1, 30.04.1994. US 2012/035075 (26.04.2012) C 2 C 2 (86) Заявка PCT: (87) Публикация заявки PCT: R U 2 5 9 ...

Liquid sampling system

In preparing a sample of a liquid material, particularly a sample suitable for chromatographic analysis, an apparatus and method are provided whereby dissolved precipitatable material is removed from the liquid material by precipitation followed by filtering to obtain a liquid sample which can be suitably analyzed. In a preferred embodiment a combination of dilution and cooling are used to precipitate trinitrotoluene from a solution containing sulfuric and nitric acids prior to the chromatographic analysis of the remaining sample liquid material.

Method of producing albumin and hemoglobin

Изобретение относитс  к медицине и касаетс  способа получени  получени  основных протеинов плацентарной гемолизированной крови. Цель изобретени  - повышение производительности процесса. Способ осуществл ют следующим образом. 150 кг человеческой плаценты дроб т в замороженном виде, диспергируют в 150 л воды и 22 л этанола при T=°С до конечной концентрации 8%, затем эту суспензию перемешивают , фильтруют, к полученной жидкости добавл ют сол ную кислоту нормальной концентрации дл  доведени  РНдо 5,1 при перемешивании T=0°С. Осветвленную кровь подвергают ультрафильтрации и после концентрации до 60 Л диафильтруют. The invention relates to medicine and relates to a method for the preparation of the main proteins of placental hemolyzed blood. The purpose of the invention is to increase the productivity of the process. The method is carried out as follows. 150 kg of human placenta are crushed in frozen form, dispersed in 150 l of water and 22 l of ethanol at T = ° C to a final concentration of 8%, then this suspension is stirred, filtered, hydrochloric acid of normal concentration is added to the resulting liquid to bring the pH to 5.1 with stirring T = 0 ° C. The clarified blood is subjected to ultrafiltration and after concentration to 60 L, it is diafiltered.

A method of measuring the concentration of hydrogen and methane in nitrogen by means of ion mobility spectrometry

본 발명은 이온 이동 분광분석기에 의해 질소 내의 메탄 및 수소의 함유량을 정량 분석하는 방법을 개시한다. 이러한 방법은, a) 검사 중에 질소 내의 겉보기 수소 농도를 측정하는 단계와, b) 메탄을 제외한 모든 불순물로부터 정화된, 동일한 표본의 질소의 흐름 중에 겉보기 수소 농도를 측정하는 단계와, 그리고 c) 이들 2개의 측정치를 비교하는 단계를 포함한다. 상술한 방법을 실행하는 시스템을 또한 개시한다. The present invention discloses a method for quantitatively analyzing the contents of methane and hydrogen in nitrogen by ion transfer spectroscopy. This method comprises the steps of a) measuring the apparent hydrogen concentration in nitrogen during the test, b) measuring the apparent hydrogen concentration in the flow of nitrogen of the same sample, purified from all impurities except methane, and c) these Comparing the two measurements. Also disclosed is a system for implementing the method described above.

Method for determining component of hdl-cholesterol- bearing liquid

Использование: медицина, биохими . Сущность изобретени : пробу биологической жидкости на средство, состо щее из двух носителей, содержащих осаждающее вещество дл  всех липопротеинов кроме HDL. Жидкость, прошедша  через носитель, содержит HDL-холестерин, В качестве носител  возможно использовать бумагу и синтетические волокна с диаметром плетени  3-100/ м и толщиной 0,03 0-0, 150 мл или стекл нных волокон, а также возможно использование гидрофильных мембран толщиной 20-250 JUM и диаметром пор 0.2-20 . При этом второй носитель выполнен из волокон с неупор доченным плетением с диаметром плетени  0,5-5,0 /IM и влагоемкостью 250-500 г/м2. 2 з.п. и ф-лы, 1 табл.

Reagent and reagent kit for analysis of immature leukocyte

본 발명은 적혈구 및 성숙 백혈구의 세포막에 손상을 주는 계면활성제와 손상된 혈구를 수축시키는 가용화제와 핵산 염색 색소를 포함하고, 침투압이 1O mOsm/kg 이상 15O mOsm/kg 미만인 유약 백혈구의 분석용 시약을 제공한다. The present invention comprises a surfactant for damaging the cell membranes of erythrocytes and mature leukocytes, a solubilizer for shrinking damaged blood cells, and a nucleic acid dye, and a reagent for analyzing glaze leukocytes having a penetration pressure of 10 mOsm / kg or more and less than 15OmOsm / kg. to provide.

Retentate chromatography and protein chip arrays with applications in biology and medicine

본 발명은 샘플내 피분석물을 분할하는 용도의 보유물 크로마토그래피 방법을 제공한다. 상기 방법은 다수의 상이한 선택 조건하에 피분석물을 기재에 흡착시키는 단계 및 기재상에 보유된 피분석물을 탈착 분광 분석에 의해 검출하는 단계를포함한다. 이 방법은 임상적 진단 및 약물 발견을 포함하는 생물학 및 의학에 유용하다. The present invention provides a retentate chromatography method for partitioning analytes in a sample. The method includes adsorbing analyte to a substrate under a number of different selection conditions and detecting analyte retained on the substrate by desorption spectroscopy. This method is useful in biology and medicine, including clinical diagnosis and drug discovery. 도 2 2

Polypeptide fingerprinting methods and bioinformatics database system

The invention provides methods, compositions, apparatus, and a computer data retrieval system for conducting proteomics.

Assay for quantifying arthritic conditions

Method for the measurement of creatine or creatinine and reagents for these measurements

A method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above, peroxidase and a color reagent for H 2 O 2 .

REAGENT AND REAGENTS FOR ANALYSIS OF IMMATURE LEUKOCYTES

1. Реактив для анализа незрелых лейкоцитов, включающий: ! поверхностно-активное вещество, которое способно повреждать клеточные мембраны эритроцитов и зрелых лейкоцитов, ! солюбилизирующее вещество, которое вызывает сжатие поврежденных клеток крови, и ! краситель для окрашивания нуклеиновой кислоты, ! причем реактив имеет осмотическое давление не ниже, чем 10 мОсм/кг и ниже, чем 150 мОсм/кг. ! 2. Реактив по п.1, где концентрация поверхностно-активного вещества в реактиве составляет от 500 до 15000 м.д. ! 3. Реактив по п.1, где поверхностно-активным веществом является полиоксиэтиленовое неионное поверхностно-активное вещество. ! 4. Реактив по п.3, где полиоксиэтиленовое неионное поверхностно-активное вещество имеет следующую формулу (I): ! ! где R1 представляет C9-25 алкильную, алкенильную или алкинильную группу; R2 представляет -O-, -COO- или ! ! и n является целым числом от 10 до 40. ! 5. Реактив по п.3, где полиоксиэтиленовым неионным поверхностно-активным веществом является полиоксиэтиленолеиловый эфир. ! 6. Реактив по п.5, где полиоксиэтиленолеиловый эфир имеет формулу (I), в которой n составляет от 15 до 20. ! 7. Реактив по п.1, где солюбилизирующее вещество выбирают их группы, состоящей из производного саркозина, производного холевой кислоты и метилглюканамида. ! 8. Реактив по п.1, где концентрация солюбилизирующего вещества в реактиве составляет от 100 до 400 м.д., когда солюбилизирующим веществом является производное саркозина; от 50 до 1300 м.д., когда солюбилизирующим веществом является производное холевой кислоты; и от 500 до 1100 м.д., когда солюбилизирующим веществом является метилглюканамид. ! 9. Реактив по п.1, где красителем для окрашивания нук РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2010 102 038 (13) A (51) МПК G01N 33/49 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2010102038/15, 25.06.2008 (71) Заявитель(и): СИСМЕКС КОРПОРЕЙШН (JP) (86) Заявка PCT: JP 2008/061572 ( ...

Cell assay kit and method

在此披露了一种用于对细胞样本测定存在至少一个阈值数目的给定类型细胞的方法和套件。该套件包括：一种测定装置，该测定装置具有一个用于接受该细胞样品的样品室、以及一个长形的收集室，该收集室含有一种选定密度和/或粘度的介质并且沿着它的长度具有多个细胞收集区，以及多个颗粒，这些颗粒能够与该选定细胞类型的细胞进行特异性连接，并且这些颗粒当与这些细胞连接时对增加这些细胞的密度或磁化率是有效的。在实施中，该细胞样品中结合颗粒的细胞以及颗粒在一种重力或所选定的离心力或磁场力的影响下被牵引穿过该长形的收集室，直到这些结合颗粒的细胞以及颗粒完全填满该收集室中的多个依次的细胞收集区。与至少一个收集区相关联的标记表示了该选定类型的细胞对于至少部分地填满该收集区是有效的。

Ascorbate intrinsic detection method of hydrogen peroxide

본 발명은, 용액을 산화-환원 지시제 및 전이 금속 착물과 접촉시킴을 포함하여, 생물학적 체액 또는 수용액 중에서 과산화수소를 검출하는 방법에 관한 것이다. 전이 금속 착물은 철 배위의 크레아티닌 또는 철 배위의구아니딘이다.

Penetrable cap

The present invention relates to a cap which can form an essentially leak-proof seal with a vessel capable of receiving fluid specimens for clinical analysis and diagnosis. To minimize potentially contaminating contact between the fluid specimen and humans or the environment, the present invention features a cap which is penetrable by a plastic pipette tip or other fluid transfer device, and may include a plurality of striations which were discovered to further improve penetrability of the cap. In this way, substances can be dispensed into or withdrawn from the vessel without having to physically separate the cap from the vessel. Also featured are fluid transfer devices and caps having surface ribs and/or grooves which aid in creating passageways for venting displaced air from a penetrated collection device.

Open platform hybrid manual-automated sample processing system

An automated sample processing system having a sample input adapted to simultaneously receive a number of sample containers, a reagent input adapted to receive one or more new reagent supplies, a consumable input adapted to receive one or more new consumable supplies, a solid waste output adapted to receive used consumable supplies, a liquid waste output adapted to receive one or more used reagent supplies, and a processing center. The processing center includes a decapper adapted to remove a lid from at least one sample container, an aspirator adapted to remove a specimen from the at least one sample container and transfer the specimen to an output vessel, and a capper adapted to replace the lid on the at least one sample container. The system also includes a sample output adapted to receive the output vessel, and a user interface adapted to receive an input from the user to indicate the identity of the at least one sample container, and control at least one operation based on a physical property of the at least one sample container.

Open platform automated sample processing system

An automated sample processing system having a sample input adapted to simultaneously receive a number of sample containers, a reagent input adapted to receive one or more new reagent supplies, a consumable input adapted to receive one or more new consumable supplies, a solid waste output adapted to receive used consumable supplies, a liquid waste output adapted to receive one or more used reagent supplies, and a processing center. The processing center includes a decapper adapted to remove a lid from at least one sample container, an aspirator adapted to remove a specimen from the at least one sample container and transfer the specimen to an output vessel, and a capper adapted to replace the lid on the at least one sample container. The system also includes a sample output adapted to receive the output vessel, and a user interface adapted to receive an input from the user to indicate the identity of the at least one sample container, and control at least one operation based on a physical property of the at least one sample container.

Array cytometry

A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled elektrokinetic assembly of particles near surfaces relies on the combination of three functional elements, the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. This invention is also for a method and apparatus to direct the lateral motion and induce the assembly into planar arrays of cells on semiconductor surfaces in response to temporally and spatially varying electric fields and to projected patterns of illumination.