High-loading-capacity multi-mode chromatography medium as well as preparation method and application thereof

The invention discloses a high-load multi-mode chromatography medium and a preparation method and application thereof, the chromatography medium comprises a chromatography substrate and a ligand, the chromatography substrate is a porous microsphere with amido, hydroxyl, carboxyl, sulfydryl or chlorine-substituted C6-20 aryl on the surface; the ligand is a hydrophobic alkenyl monomer and an alkenyl monomer with an ion exchange function, and the hydrophobic alkenyl monomer is different from the alkenyl monomer with the ion exchange function. According to the invention, the hydrophobic alkene compound and the ion exchange alkene compound are grafted and polymerized on the surface of the chromatography substrate, and the combination of more chromatography modes is realized through different types of hydrophobic alkenyl monomers and alkenyl monomers with an ion exchange function in different proportions; meanwhile, the hydrophobicity of the ligand in the prepared chromatography medium and the ...