21-07-2023 дата публикации
Номер: CN116463282A
Принадлежит:
The invention discloses a chicken mandible branch cell separation and culture method, and the primary culture method comprises the following steps: taking out a chicken mandible branch in an embryonic period under a sterile condition, rapidly putting the chicken mandible branch into PBS (Phosphate Buffer Solution) containing 1% of three antibodies, cleaning, cutting the mandible branch into 1.5 mm < 3 > after cleaning, adding 3ml of trypsin with the mass concentration of 0.25%, and pre-digesting for 20 minutes in a water bath box with the temperature of 37 DEG C; after pre-digestion is finished, adding an isovolumetric complete culture solution to terminate digestion, centrifuging to remove supernatant, then adding 5ml of I-type collagenase with the mass concentration of 0.1%, then transferring into a water bath at 37 DEG C to digest for 40 minutes, centrifuging to remove supernatant, then uniformly paving tissue blocks at the bottom of a cell culture dish, adding the complete culture solution ...
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