Display substrate, preparation method thereof and display device

The invention provides a display substrate, a preparation method thereof and a display device. The display substrate comprises a substrate body, a plurality of sub-pixels located on the substrate body, an auxiliary electrode located on the substrate body and an isolation structure located on the side, away from the substrate body, of the auxiliary electrode. The sub-pixel comprises a first electrode, a luminescent material layer and a second electrode. The isolation structure comprises a first conductive part, a second conductive part located on the side, away from the substrate, of the first conductive part, and a third conductive part located on the side, away from the substrate, of the second conductive part. The orthographic projection of the second conductive part on the substrate falls in the orthographic projection of the third conductive part on the substrate, and the area of the orthographic projection of the second conductive part on the substrate is smaller than that of the orthographic ...