25-08-2023 дата публикации
Номер: CN116640792A
Принадлежит:
The invention relates to a method for increasing the yield of xylanase in trichoderma reesei. According to the invention, a trichoderma reesei endogenous protein Xyn2 is used as a fusion expression element, the fusion expression element Xyn2 and a coding gene of xylanase Xyn10A are connected together to form a fusion protein gene, then the fusion protein is subjected to heterologous expression, and xylanase is synthesized. The nucleotide sequence of the fusion expression element Xyn2 is as shown in SEQ ID NO.2, and the amino acid sequence of the fusion expression element Xyn2 is as shown in SEQ ID NO.1. The enzyme activity of the xylanase obtained by the method is obviously higher than that of the xylanase Xyn10A which is directly expressed, the enzyme activity of the xylanase produced by the recombinant trichoderma reesei xyn2-xyn10A by taking glucose as a carbon source reaches 170U/mL, and the enzyme activity of the xylanase produced by the strain by taking cellulose as a carbon source ...
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