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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 13. Отображено 13.
15-08-2023 дата публикации

Chimeric antigen receptor co-expressing FOSB and application thereof

Номер: CN116589598A
Автор: ZHANG TONGCUN
Принадлежит:

The invention discloses a chimeric antigen receptor co-expressing FOSB and application of the chimeric antigen receptor. The chimeric antigen receptor is sequentially spliced with a signal peptide, a single-chain antibody ScFv targeting CD19, strepII, CD8hinge, a CD28 transmembrane region, a CD28 intracellular structural domain, an intracellular co-stimulatory domain 4-1BB and a CD3 zeta chain from the N end to the C end. And a P2A peptide and FOSB are further spliced at the C end of the CD3 zeta chain. Taking a CD19 antibody as an example, the anti-CD19 chimeric antigen receptor co-expressing the FOSB is used for modifying immune cells, and the killing effect of the modified immune cells on tumor cells is superior to that of common Anti-CD19 CAR-T cells.

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Номер записи: 1
16-04-2020 дата публикации

FUMONISIN DEGRADING ENZYME FUMDPS AND GENE AND USE THEREOF

Номер: WO2020073845A1
Автор: LI, Zhongyuan, ZHANG, Tongcun, ZHAO, Junqi, LIU, Huihui
Принадлежит:

Provided is a fumonisin degrading enzyme protein FumDPS derived from Pigmentiphaga sp. The amino acid sequence thereof is as shown in SEQ ID NO. 1. Further provided is a coding gene coding said fumonisin degrading enzyme. The fumonisin degrading enzyme FumDPS has the activity of degrading fumonisin B1, has an optimum pH of 8.0-9.0, and an optimum reaction temperature of 37 °C.

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Номер записи: 2
30-05-2023 дата публикации

Freezing centrifuge for CD30 positive lymphoma antibody test

Номер: CN116174172A
Автор: XIA XIANGCHANG, ZHOU JUN, LI SHENGHUA, HU JIALING, ZHANG TONGCUN, HU JIA
Принадлежит:

The invention relates to the technical field of centrifugal equipment, and provides a refrigerated centrifuge for a CD30 positive lymphoma antibody test, which comprises a shell and a cover, a centrifugal bin is arranged on the outer surface of the shell, a centrifugal disc is arranged in the centrifugal bin, a circulating structure is arranged in the centrifugal bin close to the centrifugal disc, an inner cover plate is movably arranged on the outer surface of the centrifugal disc, and the inner cover plate is arranged on the outer surface of the centrifugal disc. An embedding structure is arranged between the inner cover plate and the centrifugal disc, a sealing structure is arranged between the machine cover and the machine shell, and the outer surface of the machine cover is fixedly connected with a jacking block; the embedded structure comprises a clamping groove formed in the outer surface of the centrifugal disc, a clamping hole is formed in the side, corresponding to the clamping ...

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Номер записи: 3
25-04-2023 дата публикации

Lactobacillus delbrueckii for producing exopolysaccharide with antioxidant effect, polysaccharide, method and application of lactobacillus delbrueckii

Номер: CN116004443A
Автор: WANG NAN, ZHANG TONGCUN, ZHAO CHONGJIE, SHEN JING, XIE LINLIN, GE SAILUN, HE HONGPENG, ZUO FEIFEI, LUO XUEGANG
Принадлежит:

The invention discloses a strain of Lactobacillus delbrueckii capable of producing exopolysaccharides with an anti-oxidation effect, the name of the Lactobacillus delbrueckii is KY20, the classification name is Lactobacillus delbrueckii subsp. Bulgaricus, the preservation number is CGMCC No.20084, the preservation date is Institute of Biology, Chinese Academy of Sciences, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, June 15, 2020, the preservation number is CGMCC No.20084, and the preservation number is CGMCC No.20084. The preservation unit is China General Microbiological Culture Collection Center (CGMCC). The lactobacillus delbrueckii disclosed by the invention has good gastrointestinal tract environment tolerance, and the exopolysaccharide produced by the lactobacillus delbrueckii contains glucose, carboxyl and intermolecular and intramolecular hydrogen bonds and has an antioxidant function.

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Номер записи: 4
03-01-2019 дата публикации

CONSTRUCTION OF RECOMBINED GENE OF CHIMERIC ANTIGEN RECEPTOR FOR TREATMENT OF HIV INFECTION, AND APPLICATION THEREOF

Номер: WO2019000620A1
Автор: ZHANG, Tongcun, GU, Chaojiang, LIAO, Xinghua
Принадлежит:

Provided is a single-chain antibody ScFv capable of recognizing gp120 on the surface of cells infected with HIV and an application thereof. The antibody is obtained by tandemly linking antibody light chain and heavy chain variable regions of gp120 on the surface of cells infected with HIV. The single-chain antibody is prepared into a chimeric antigen receptor (CAR), a CAR coding gene is transferred into a plasmid vector, and the lentiviral vector, into which the CAR coding gene is inserted, is transduced into CD8+ T lymphocytes. The resultant CAR-T lymphocytes exhibit inhibition and killing activity with respect to HIV-infected cells in both in vitro and in vivo experiments, and can be used for the preparation of anti-HIV infection drugs.

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Номер записи: 5
12-05-2023 дата публикации

Fusion chimeric antigen receptor modified T cell for targeted expression of GPR87 surface antigen cell and application thereof

Номер: CN116102661A
Автор: ZHANG TONGCUN, SUN FAN, CUI YETING, LYU MENGQING
Принадлежит:

The invention provides a fusion chimeric antigen receptor for targeted expression of GPR87 surface antigen cells and application of the fusion chimeric antigen receptor, and belongs to the technical field of gene engineering. The chimeric antigen receptor is at least one of (1) a chimeric antigen receptor for targeted expression of GPR87 surface antigen cells; (2) expressing GPR87 surface antigen cells in a targeted manner, and simultaneously secreting IL-15SA fusion protein, IL-36 gamma or a recruitment chimeric antigen receptor of CXCL11; (3) carrying out targeted expression on GPR87 surface antigen cells, and simultaneously carrying out membrane expression on an infiltrated chimeric antigen receptor of CXCR4 or CXCR6; and (4) performing targeted expression on GPR87 surface antigen cells, secreting IL-15SA fusion protein, IL-36 gamma or CXCL11, and performing membrane expression on a fusion chimeric antigen receptor of CXCR4 or CXCR6 at the same time. The invention provides an efficient ...

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Номер записи: 6
07-04-2023 дата публикации

Establishment and application of method for detecting pathogenic microorganisms in CAR-T cell product based on mNGS

Номер: CN115927565A
Автор: ZHANG TONGCUN, LIU YU, ZHU HAICHUAN, WU HAN, SUN FAN
Принадлежит:

The invention provides a method for detecting pathogenic microorganisms in a CAR-T (Chimeric Antigen Receptor-T) cell product based on an mNGS (Metagenome sequencing) technology and application of the method, and belongs to the technical field of gene detection. The invention discloses a method for detecting pathogenic microorganisms in a CAR-T cell product based on an mNGS technology. The method comprises the following steps: S1, extracting nucleic acid of the CAR-T cell product based on mNGS detection; s2, preparing a library for detecting a CAR-T cell product based on mNGS; s3, detecting the sequencing of the CAR-T cell product based on mNGS; and S4, analyzing sequencing data of the CAR-T cell product. The invention establishes a detection method which is efficient, standard and easy to standardize and can be used for rapidly releasing the CAR-T cell product. By greatly shortening the detection time of the exogenous pathogenic microorganism risk factors of the CAR-T cell product, the ...

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Номер записи: 7
15-08-2023 дата публикации

Selenium-rich nostoc extract, pharmaceutical composition, preparation method and application thereof

Номер: CN116585363A
Автор: ZHANG TONGCUN, HE XIAOHONG, YUAN FAHU
Принадлежит:

The invention provides a pharmaceutical composition. The pharmaceutical composition comprises a selenium-rich nostoc extract and an AIDS auxiliary drug. The pharmaceutical composition provided by the invention has good biological activity and antiviral effect, can effectively inhibit the replication and infection of HIV-1 and improve the immunity of the organism, and has no toxic or side effect.

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Номер записи: 8
12-05-2023 дата публикации

Fusion chimeric antigen receptor targeting CA9, recombinant expression vector and application of fusion chimeric antigen receptor

Номер: CN116102662A
Автор: ZHANG TONGCUN, SUN FAN, LYU MENGQING, CUI YETING
Принадлежит:

The invention provides a fusion chimeric antigen receptor targeting CA9, a recombinant expression vector and application thereof, and belongs to the technical field of gene engineering. The invention relates to a fusion chimeric antigen receptor for expressing CA9 surface antigen cells in a targeted manner, secreting IL-15SA fusion protein or IL-36gamma and CXCL11 and expressing CCR8 or CXCR6 on a membrane, which comprises a CD8 alpha signal peptide, CA9-targeted scFv, Strep II tag protein, a CD8 alpha hinge domain, a CD28 transmembrane structural domain, a CD28 intracellular signal structural domain, a 41-BB costimulatory domain, a CD3 zeta signal transduction domain and a structural domain for secreting IL-15SA, IL-36gamma or CXCL11 fusion protein, and the membrane expresses a structural domain of CCR8 or CXCR6. The fusion chimeric antigen receptor is expressed in T cells, immune cell infiltration is enhanced, more immune cells are collected, and a better tumor inhibition effect is achieved ...

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Номер записи: 9
26-05-2023 дата публикации

Chimeric antigen receptor taking CD244 antigen as target spot as well as preparation method and application of chimeric antigen receptor

Номер: CN116162170A
Автор: ZHANG TONGCUN, HUANG ZICHENG, LIU YU, CHAO ZIJIAN
Принадлежит:

The invention provides a chimeric antigen receptor (CAR) taking a CD244 antigen as a target spot as well as a preparation method and application of the chimeric antigen receptor, and belongs to the technical field of chimeric antigen receptors. A polynucleotide sequence of the chimeric antigen receptor taking the CD244 antigen as the target spot is sequentially spliced with a signal peptide, a CD48 extracellular domain (CD48 extracellular domain, hereinafter referred to as CD48ECD), a CD8 hinge, a CD28 transmembrane region, a CD28 intracellular domain, an intracellular costimulatory domain 4-1BB and a CD3 zeta chain from an N end to a C. According to the chimeric antigen receptor, a ligand CAR-T cell carrying the targeted CD244 is constructed, tumor cells with surface expression of the CD244 can be efficiently and specifically killed, and the chimeric antigen receptor has the advantages that the chimeric antigen receptor can be used for killing tumor cells with surface expression of the ...

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Номер записи: 10
01-08-2023 дата публикации

CD19 targeting chimeric antigen receptor with optimized signal peptide sequence and application of CD19 targeting chimeric antigen receptor

Номер: CN116514992A
Автор: ZHANG TONGCUN
Принадлежит:

The invention discloses optimization and application of a chimeric antigen signal peptide taking CD19 as a target spot, and the optimized signal peptide can significantly improve the expression efficiency and stability of a chimeric antigen receptor taking a CD19 receptor as a target spot in T cells, thereby better playing a role in killing target cells.

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Номер записи: 11
23-06-2023 дата публикации

Targeting CD200 chimeric antigen receptor and application thereof

Номер: CN116284446A
Автор: ZHANG TONGCUN, TIAN GAOHUI
Принадлежит:

The invention provides a targeting CD200 chimeric antigen receptor and an application thereof. The targeting CD200 chimeric antigen receptor comprises a signal peptide, a single-chain antibody ScFv, strepII, a CD8 alpha hinge, a CD28 transmembrane region, a CD28 intracellular structural domain, an intracellular costimulatory domain 4-1BB and a CD3 zeta chain which are sequentially spliced from an N terminal to a C terminal, wherein the signal peptide, the single-chain antibody ScFv, the strepII, the CD8 alpha hinge, the CD28 transmembrane region, the CD28 intracellular structural domain, the intracellular costimulatory domain 4-1BB and the CD3 zeta chain are sequentially spliced from the N terminal to the C terminal; the single-chain antibody ScFv specifically recognizes a CD200 antigen on the surface of a tumor cell. The chimeric antigen receptor provided by the invention has a remarkable effect in preparation of drugs for treating acute myelogenous leukemia.

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Номер записи: 12
06-06-2023 дата публикации

Pediococcus acidilactici exopolysaccharide with antioxidant, antibacterial and antitumor activity, method and application of Pediococcus acidilactici exopolysaccharide

Номер: CN116218930A
Автор: WANG NAN, ZHANG TONGCUN, JIANG JIAYUN, ZHAO CHONGJIE, LI XUENING, HE HONGPENG, LIU MEILING, ZUO FEIFEI
Принадлежит:

The invention discloses a pediococcus acidilactici extracellular polysaccharide with antioxidant activity, antibacterial activity and anti-tumor activity. The extracellular polysaccharide is an extracellular polysaccharide produced by pediococcus acidilactici which is an extracellular polysaccharide with antioxidant activity, antibacterial activity and anti-tumor activity. The extracellular polysaccharide with high yield of the pediococcus acidilactici HAO2018 disclosed by the invention has good antioxidant activity, antibacterial activity and anti-tumor activity. The extracellular polysaccharide produced by the pediococcus acidilactici disclosed by the invention has remarkable antioxidant capacity, and can be used for preparing medicines, foods, health-care products, cosmetics and microecological preparations with antioxidant effects. The extracellular polysaccharide produced by the pediococcus acidilactici disclosed by the invention can be used for preparing the medicines, the foods, ...

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Номер записи: 13