Hydroxylase capable of efficiently hydroxylating 9 alpha position of 4-androstene-3-ketone series compound and application of hydroxylase

The invention relates to the technical field of biology, in particular to efficient hydroxylation 4-androstene-3-ketone series compound 9 alpha-site hydroxylase and application thereof, the amino acid sequence of the hydroxylase is shown as SEQ ID NO: 1, the nucleotide sequence of the hydroxylase is shown as SEQ ID NO: 2, and the hydroxylase can produce 9 alpha-hydroxy-4-androstene-3-ketone compounds. The C9 alpha hydroxylase KshA substrate recognition is wide, the 9 alpha-hydroxy-4-androstene-3-ketone compound is high in yield, the purification process is not limited by co-expression of a reducing component KshB, and the C9 alpha hydroxylase KshA can be used for producing the 9 alpha-hydroxy-4-androstene-3-ketone compound in vitro. The invention further provides an engineering bacterium for producing the 9 alpha-hydroxy-4-androstene-3, 17-diketone, and the engineering bacterium has the advantages that the C9 alpha hydroxylase KshA substrate recognition is wide, and the C9 alpha hydroxylase ...