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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 19. Отображено 19.
06-06-2023 дата публикации

Method for improving thermal stability of alkaline protease, mutants ThAPT3-M1 and ThAPT3-M2 and application of mutants ThAPT3-M1 and ThAPT3-M2

Номер: CN116218821A
Автор: LUO HUIYING, WANG XIAO, QIN XING, YAO BIN, WANG YARU, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, WANG YUAN, TU TAO, ZHANG JIE, WANG XIAOLU, ZHANG HONGLIAN, YU HUIMIN
Принадлежит:

The invention relates to the field of gene engineering and protein engineering. In particular to a method for improving the thermal stability of alkaline protease, mutants ThAPT3-M1 and ThAPT3-M2 and application of the mutants ThAPT3-M1 and ThAPT3-M2. According to the invention, alkaline protease PA3 is used as a parent, and the protease mutant with significantly improved thermal stability is obtained through segment substitution and site-specific mutagenesis. After the parent PA3 is treated for 10 minutes and 20 minutes under the condition of 60 DEG C, the residual enzyme activities are 1.9 percent and 1.6 percent respectively. After the mutants ThAPT3-M1 and ThAPT3-M2 are treated for 10 minutes at the temperature of 60 DEG C, the residual enzyme activities are 62% and 69% respectively; after the mutants ThAPT3-M1 and ThAPT3-M2 are treated for 20 minutes at the temperature of 60 DEG C, the residual enzyme activities are 39% and 43% respectively. In addition, the specific activity of the ...

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Номер записи: 1
04-08-2023 дата публикации

Method for constructing high-yield gamma-aminobutyric acid recombinant escherichia coli and method for producing gamma-aminobutyric acid

Номер: CN116536341A
Автор: WANG XIAOLU, CHANG FANGFANG, ZHANG JIE, TU TAO, YAO BIN, LUO HUIYING, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, ZHANG HONGLIAN, QIN XING, WANG YUAN, WANG YARU, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a method for constructing high-yield gamma-aminobutyric acid recombinant escherichia coli and a method for producing gamma-aminobutyric acid. According to the invention, an escherichia coli engineering strain capable of producing gamma-aminobutyric acid is constructed by heterologous expression of a glutamic acid decarboxylase coding gene from bacillus Z11 in escherichia coli. In order to eliminate the dependence of the escherichia coli engineering strain on an exogenous expensive cofactor pyridoxal 5 '-phosphoric acid in the process of producing gamma-aminobutyric acid by transforming a substrate, a synthesis route of endogenous pyridoxal 5'-phosphoric acid is constructed in the escherichia coli engineering strain by screening a gene source, a gene combination and an expression regulation mode. The construction of the approach not only can completely supply the coenzyme demand of the glutamate decarboxylase ...

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Номер записи: 2
06-07-2016 дата публикации

Milling machine and walking control system thereof

Номер: CN0205368984U
Автор: HU BIN, JIA WEIBIN, WANG XIAOLU, SANG GUANGBO, HAO SHOUQING
Принадлежит:

... 本实用新型提供了一种铣刨机及其行走控制系统，所述的铣刨机行走控制系统包括铣刨机的行走操纵手柄和液压执行机构、用于实时采集铣刨机的行走速度的转速传感器，及前进电比例阀、后退电比例阀、所述行走操纵手柄带有的角度传感器、用于与所述角度传感器配合使用的计时单元，及基于所述转速传感器、角度传感器和计时单元采集的相关信息值计算铣刨机当前次调速后的行走速度V、并控制铣刨机在其行走操纵手柄在所述当前次调速后自动复位过程中以该计算出的行走速度V进行行走的控制器，所述的前进电比例阀和后退电比例阀均与所述的液压执行机构相连。该方案能在一定程度上解放操作者的双手，减轻铣刨机操作者的工作负荷，提高操作者的工作效率。 ...

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Номер записи: 3
23-06-2023 дата публикации

Heat-resistant alpha-amylase mutant with high specific activity as well as gene and application thereof

Номер: CN116286748A
Автор: TU TAO, LIAO MIN, LUO HUIYING, YAO BIN, HUANG HUOQING, BAI YINGGUO, WANG YUAN, SU XIAOYUN, WANG YARU, ZHANG JIE, QIN XING, WANG XIAOLU, ZHANG HONGLIAN, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a heat-resistant alpha-amylase mutant with high specific activity and a gene and application thereof. According to the present invention, A262D single point mutation is performed on the mutant alpha-amylase K152H/A166C/E168H (M1) with the amino acid sequence represented by SEQ ID NO. 1 so as to obtain the mutant of the alpha-amylase, wherein the A262D single point mutation is performed on the mutant alpha-amylase K152H/A166C/E168H (M1), compared with alpha-amylase M1, the alpha-amylase mutant disclosed by the invention has the advantages that the specific activity is greatly improved, and the optimum temperature is not changed and is 95 DEG C.

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Номер записи: 4
05-05-2023 дата публикации

Natural antifreeze peptide as well as preparation method and application thereof

Номер: CN116064709A
Автор: WANG SHUXIAN, GAI CHUNLEI, FAN YING, DIAO JING, CHYLA, WANG XIAOLU, WANG YOUHONG, YU XIAOQING, YE HAIBIN, WEI JIANTENG
Принадлежит:

The invention provides a natural antifreeze peptide and a preparation method and application thereof.The preparation method of the antifreeze peptide comprises the steps that fish roes are subjected to enzymolysis through ficin and alkaline protease, supernate is collected from enzymolysis products, the supernate is filtered through a 10kDa membrane, filtrate is filtered through a 1000Da membrane, and trapped fluid is collected; and carrying out spray drying on the trapped fluid to prepare the antifreeze peptide. The antibacterial peptide with high thermal hysteresis activity prepared by the invention is added into a staphylococcus aureus bacterial liquid, bacterial colony count and bacterial liquid OD600 are measured through a bacterial low-temperature protection experiment, and research finds that compared with an antibacterial peptide treatment group, the bacterial survival rate of blank control and negative control is remarkably reduced, and the antibacterial activity of the antibacterial ...

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Номер записи: 5
09-05-2023 дата публикации

Method for constructing Ralstonia engineering bacteria for producing inositol by taking glucose, glycerol and CO2 as carbon sources and strain

Номер: CN116083468A
Автор: WANG XIAOLU, ZHANG JIE, YAO BIN, LUO HUIYING, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, TU TAO, ZHANG HONGLIAN, WANG YUAN, WANG YARU, QIN XING, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a method for constructing Ralstonia engineering bacteria for producing inositol by taking glucose, glycerol and CO2 as carbon sources and a strain. According to the application, a gene nagE for coding an N-acetylglucosamine specific phosphotransferase system in a Ralstonia genome is mutated (G265R), and nagR for coding a transcriptional regulatory factor of a GntR family is knocked out, so that the Ralstonia can efficiently utilize glucose. On the basis, a Ralstonia H16 engineering strain for synthesizing inositol by using renewable substrates (glucose and glycerol) and directly using CO2 as a carbon source is constructed. Based on the technology, synthesis of bioactive substances can be achieved, and meanwhile the method is a method for efficiently eliminating greenhouse gas in the atmosphere.

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Номер записи: 6
15-08-2023 дата публикации

Biological reaction tank with comprehensive pipe rack structure arrangement

Номер: CN116589097A
Автор: GAO YUAN, WANG XIAOLU, PENG XIANGCONG, XU SHUAIXIAN, XIE YONG
Принадлежит:

The invention discloses a biological reaction tank with comprehensive pipe rack structure arrangement, which comprises two groups of symmetrically arranged biological tanks, and a comprehensive pipe rack structure is arranged between the two groups of biological tanks; an air pipe, a deodorization air pipe and a sludge return pipe are arranged in the comprehensive pipe rack structure; the sludge return pipe penetrates through the side wall of the comprehensive pipe rack structure and is connected with the anaerobic tank and the aerobic tank of the corresponding biological tank; and the air pipe and the deodorization air pipe are connected into the aerobic tank through a pipe ditch which is horizontally intersected with the comprehensive pipe rack structure. According to the application of the system, various pipelines in the biological tank can be laid more reasonably and effectively, complex operation and maintenance work of an existing biological tank is simplified, and various pipelines ...

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Номер записи: 7
31-05-2018 дата публикации

MOVABLE BALLAST LEVELING CONTROL DEVICE FOR USE IN FLOATING WIND TURBINE

Номер: WO2018095304A1
Автор: LI, Chao, XIAO, Yiqing, ZHOU, Shengtao, HAN, Xishuang, LIU, Haitao, WANG, Xiaolu
Принадлежит:

A movable ballast leveling control device for use in a floating wind turbine, comprising: a movable ballast (2), a sliding rail (1), a limiting support seat (3), a leveling control server and so on; the movable ballast (2) is provided with power, brake and locking systems, and may receive signals in real time in order to slide, brake and lock on the sliding rail (1). The sliding rail (1) is configured according to the basic layout and available space of a floating wind turbine, and is disposed with rails, hinges, lead screws and so on, which are required for the ballast to move. The limiting support seat (3) is provided at an end portion of the sliding rail (1), which is used for preventing the movable ballast (2) from sliding out of the rail, and for placing auxiliary equipment for the power of the ballast. The leveling control server may be installed in a foundation deck or a wind turbine tower drum, monitor data according to the speed and direction of wind, wave, and flow, and send real-time ...

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Номер записи: 8
04-07-2023 дата публикации

SOD (superoxide dismutase) recombinant plasmid with VEC (vascular endothelial cell) cell-penetrating peptide, fusion protein and construction method

Номер: CN116376903A
Автор: WU YI, WANG XIAOLU, LIN ZHUOYING, CHEN JIANZHOU, YAO WENYU, JIANG RENWANG
Принадлежит:

The invention provides recombinant construction and application of a superoxide dismutase fusion protein with VEC (vascular endothelial cell) cell-penetrating peptide. The invention provides a specific primer constructed by recombination of the superoxide dismutase fusion protein with the VEC cell-penetrating peptide, a superoxide dismutase recombinant plasmid with the VEC cell-penetrating peptide prepared by using the primer, and the superoxide dismutase fusion protein with the VEC cell-penetrating peptide. The fusion protein disclosed by the invention can effectively penetrate through cell membranes, plays an anti-oxidation role in cells, can more effectively improve the skin aging symptom and better solve the skin aging problem, and in addition, the fusion protein disclosed by the invention can promote angiogenesis and plays an important role in wound healing.

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Номер записи: 9
01-08-2023 дата публикации

Heat pump rectification system and method for DMC synthesis

Номер: CN116510334A
Автор: SHI SHAOJUN, LIU CHANGFENG, LIAN XIAOSONG, SUN YUAN, WANG XIAOLU, ZHANG MINGZHU, HUANG JIEQIN, ZHENG TIANMIN, CAO YAN
Принадлежит:

The invention discloses a heat pump rectification system and method for DMC synthesis. The heat pump rectification system comprises a rectification tower, a tower top evaporator, a flash tank and a heat pump compressor unit. The rectifying tower comprises a tower top and a tower kettle; the tower top evaporator is provided with a first input end, a second input end and a first output end, and the first input end is communicated with the tower top; the flash tank comprises a third input end and a second output end, the third input end is communicated with the tower kettle, and the second output end is communicated with the second input end; the heat pump compressor unit comprises a fourth input end and a third output end, the fourth input end is communicated with the first output end, and the third output end is communicated with the tower kettle. According to the heat pump rectification method, the heat pump rectification system is adopted, the tower top gas phase serves as a heat source ...

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Номер записи: 10
16-05-2023 дата публикации

Beta-1, 3-glucanase mutant N54W as well as gene and application thereof

Номер: CN116121227A
Автор: TU TAO, WANG YUAN, GAO FANG, LUO HUIYING, YAO BIN, HUANG HUOQING, BAI YINGGUO, WANG YARU, ZHANG JIE, QIN XING, WANG XIAOLU, SU XIAOYUN, ZHANG HONGLIAN, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a beta-1, 3-glucanase mutant N54W as well as a gene and application thereof. The amino acid sequence of the beta-1, 3-glucanase N54W mutant is as shown in SEQ ID NO: 2. Compared with wild type beta-1, 3-glucanase, the beta-1, 3-glucanase mutant has the advantages that the specific activity of the beta-1, 3-glucanase is increased by 3.6 times and reaches 902.1 U/mg, the catalytic activity is greatly improved, and kcat/Km reaches 888.9 ml.mg <-1 >. Min <-1 > and reaches 5.5 times of that of the wild type. The invention provides application of the beta-1, 3-glucanase mutant with high specific activity, and the beta-1, 3-glucanase mutant can be specifically applied to the fields of brewing, food and feed and has very wide application prospects.

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Номер записи: 11
05-05-2023 дата публикации

Method for improving thermal stability of alkaline protease, mutants ThAPT3-M3 and ThAPT3-M4 and application

Номер: CN116064490A
Автор: LUO HUIYING, WANG XIAO, QIN XING, YAO BIN, WANG YARU, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, WANG YUAN, TU TAO, ZHANG JIE, WANG XIAOLU, ZHANG HONGLIAN, YU HUIMIN
Принадлежит:

The invention relates to the field of gene engineering, in particular to a method for improving thermal stability of alkaline protease, mutants ThAPT3-M3 and ThAPT3-M4 and application of the mutants ThAPT3-M4. Alkaline protease PA3 is taken as a parent, and the protease mutant with remarkably improved thermal stability is obtained through site-specific mutagenesis. After the parent PA3 is treated for 10 minutes and 20 minutes under the condition of 60 DEG C, the residual enzyme activities are 1.9 percent and 1.6 percent respectively. After the mutants ThAPT3-M3 and ThAPT3-M4 are treated for 10 minutes at the temperature of 60 DEG C, the residual enzyme activities are 58% and 68% respectively; after the mutants ThAPT3-M3 and ThAPT3-M4 are treated for 20 minutes at the temperature of 60 DEG C, the residual enzyme activities of the mutants ThAPT3-M3 and ThAPT3-M4 are 31% and 45% respectively. In addition, the specific activity of the mutant ThAPT3-M4 is improved by 25%. Therefore, compared ...

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Номер записи: 12
21-07-2023 дата публикации

Method for improving enzyme activity of manganese peroxidase and hydrogen peroxide tolerance, mutant, coding gene and application

Номер: CN116463308A
Автор: TU TAO, LIU XIAOQING, WANG XINYUAN, LUO HUIYING, YAO BIN, HUANG HUOQING, BAI YINGGUO, WANG YARU, WANG YUAN, ZHANG JIE, QIN XING, WANG XIAOLU, SU XIAOYUN, ZHANG HONGLIAN, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a method for improving enzyme activity of manganese peroxidase and hydrogen peroxide tolerance, a mutant, a coding gene and application. The manganese peroxidase MrMnP derived from Moniliophora rori is used as a female parent, and the manganese peroxidase MrMnP1 of which the catalytic efficiency and the H2O2 tolerance are improved is obtained. The related amino acid mutation point is Glu148Gly. The enzyme activity of the modified manganese peroxidase MrMnP1 is improved from 10.43 U/mg to 13.83 U/mg, and the improvement amplitude is 32.7%; under 1.5 mM of H2O2, the enzyme activity of the MrMnP1 is improved to 8.3 U/mg from 4.3 U/mg, and the improvement amplitude is 92.7%; after the MrMnP1 is treated for 30 minutes under 1.5 mM H2O2, the residual enzyme activity of the MrMnP1 is improved from 0.13 U/mg to 1.53 U/mg, and the improvement amplitude is 1067%.

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Номер записи: 13
21-04-2023 дата публикации

Application of 4-hydroxy-2-pyridone alkaloid in preparation of medicine for treating gastric cancer

Номер: CN115990162A
Автор: WANG JUNJIAN, ZHANG CHENXI, WANG HONG, CAI GUODI, WANG XIAOLU
Принадлежит:

The invention relates to application of 4-hydroxy-2-pyridone alkaloid in preparation of a medicine for treating gastric cancer. The 4-hydroxy-2-pyridone alkaloid has a structure as shown in a formula (I). The 4-hydroxy-2-pyridone alkaloid with the specific structure has a good effect of inhibiting an mTORC1 signal channel and also has a good effect of inhibiting an mTORC1 signal channel. The apatinib compound has the effect of enhancing the sensitivity of apatinib to the gastric cancer, so that the apatinib compound has good activity for treating the gastric cancer, meets the requirement of medication safety at the dosage for exerting the drug effect, and can be used for preparing the drug for treating the gastric cancer.

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Номер записи: 14
28-07-2023 дата публикации

Text keyword extraction method combined with context semantics

Номер: CN116502637A
Автор: WANG XIAOLU
Принадлежит:

The invention relates to a text keyword extraction method combined with context semantics, which is characterized by comprising the following steps of: S1, acquiring text data, and performing generalization processing on numerical value and time type data; s2, preprocessing the text data to obtain a preprocessed text; s3, extracting a plurality of head words from the preprocessed text, establishing a model according to a preset sliding window size, and obtaining context words of each head word in the preprocessed text; s4, constructing a plurality of central word lists and context representation matrixes of the central word lists according to the central words and the context words; s5, performing dimension reduction processing on each context representation matrix, and extracting context semantic feature vectors of words in each central word list; s6, keyword weights of all the head words are obtained according to the context semantic feature vectors; and S7, determining the head word ...

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Номер записи: 15
27-07-2016 дата публикации

Milling machine controlling means

Номер: CN0205399164U
Автор: HU BIN, MA QIAN, LIU XIFENG, SANG GUANGBO, WANG XIAOLU
Принадлежит:

... 本实用新型公开了种铣刨机控制装置，包括控制铣刨机动作且位于铣刨机内部的控制器，以及位于铣刨机上相对两端的第操作台和第二操作台，所述第操作台和第二操作台上分别设有电连接于控制器的功能按钮，设有所述功能按钮的第操作台和第二操作台上分别设有个与该功能按钮串联的状态指示灯。每个操作台的功能按钮均与控制器电连接，通过控制器内部的互锁电路避免操作冲突，并可通过状态指示灯判断哪个操作台上的功能按钮被按下了；通过任操作台上的停止按钮均可使铣刨机停止动作，且可通过工作指示灯判断当前铣刨机的工作状态；提高整车的智能化水平以及安全性能。 ...

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Номер записи: 16
29-08-2023 дата публикации

Method for constructing Ralstonia engineering bacteria for producing N-acetylglucosamine by taking fructose, glycerol and CO2 as carbon sources

Номер: CN116656711A
Автор: WANG XIAOLU, ZHANG JIE, YAO BIN, LUO HUIYING, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, TU TAO, ZHANG HONGLIAN, WANG YUAN, WANG YARU, QIN XING, YU HUIMIN
Принадлежит:

The invention relates to the technical field of agricultural biology, in particular to a method for constructing Ralstonia engineering bacteria for producing N-acetylglucosamine by taking fructose, glycerol and CO2 as carbon sources. According to the invention, nagC, nagE, nagF, nagA and nagB genes in Ralstonia H16 are knocked out to block N-acetylglucosamine from entering cells and a catabolism pathway of N-acetylglucosamine in the cells. And then, a glucosamine-6-phosphate N-acetyltransferase mutant gene (Cegna1 *) derived from caenorhabditis elegans is subjected to heterologous expression, so that an N-acetylglucosamine production strain is constructed. The yield of N-acetylglucosamine is further increased by blocking an ED (Ethylenediamine) pathway and a polyhydroxybutyrate (PHB) synthesis pathway. Fermentation is carried out by taking fructose, glycerol and CO2 as unique carbon sources, and the highest yields of N-acetylglucosamine respectively reach 199.9 mg/L, 566.3 mg/L and 75.3 ...

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Номер записи: 17
06-06-2023 дата публикации

Method for improving thermal stability of alkaline protease, mutant ThAPT3-M5 and application

Номер: CN116218822A
Автор: ZHANG HONGLIAN, WANG XIAO, LUO HUIYING, QIN XING, YAO BIN, WANG YARU, HUANG HUOQING, BAI YINGGUO, SU XIAOYUN, WANG YUAN, TU TAO, ZHANG JIE, WANG XIAOLU, YU HUIMIN
Принадлежит:

The invention relates to the field of gene engineering and protein engineering. In particular to a method for improving the thermal stability of alkaline protease, a mutant ThAPT3-M5 and application of the mutant ThAPT3-M5. According to the invention, alkaline protease PA3 is taken as a parent, and the protease mutant ThAPT3-M5 with significantly improved thermal stability is obtained. The Tm value of the parent PA3 is 68.2 +/-0.3 DEG C, and the Tm value of the mutant is increased to 69.5 +/-0.2 DEG C. The t1/2 of the mutant at 55 DEG C and 60 DEG C is respectively increased to 2.3 times and 2.8 times of the t1/2 of the parent. After treatment at 60 DEG C for 10 min, the residual enzyme activity of the parent is 1.9%, and the residual enzyme activity of the mutant M5 is 34.9%. In addition, the specific activity of the mutant is improved from the initial 3078.3 +/-82.5 U/mg to 3701.9 +/-42.5 U/mg. Therefore, compared with a wild type, the alkaline protease mutant provided by the invention ...

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Номер записи: 18
05-05-2023 дата публикации

Multi-color combined container sticker automatic printing machine

Номер: CN116061552A
Автор: LI JIUJIANG, YANG HUA, MOON GEUN-RIM, HUANG SHAOYUN, YAO LIANG, CHEN CHANGJIANG, HUANG WEN, YAO CHONG, CHEN RIXIONG, FANG HAOJUN, NING XIAOQIANG, CAI XINQUAN, XIANG SHENG, WANG YOUGUI, JIANG BAO, WANG XIAOLU, SUN YONGJIAN, MENG HAO, GONG JING, ZHU KONGJIN, YU MIAO, TANG ZHIHUA, WEN HUADONG, JING SIYUAN, CHEN JUN, PANG SHUAI, ZHOU ZHENGQIANG, MEI WENDI, YU LEI
Принадлежит:

The invention provides a multi-color combined container sticker automatic printing machine, and relates to the field of printing machines. The container sticker clamping assembly is mounted at the top of the bottom plate; the number of the positioning assemblies is two, and the two positioning assemblies are installed on the front side and the rear side of the container sticker clamping assembly. The container sticker tensioning assembly is installed on the top of the bottom plate. The container sticker driving mechanism is mounted at the top of the bottom plate; the container sticker cutting assembly is mounted at the top of the bottom plate; the container sticker collecting assembly is mounted at the top of the bottom plate; the number of the clamping frames is two, and the two clamping frames are fixedly installed on the top of the bottom plate. The container sticker body is tensioned, so that the container sticker body is in close contact with a multi-color printing machine body; the ...

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Номер записи: 19