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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 26. Отображено 26.
29-12-2016 дата публикации

INSPECTION SYSTEM, INSPECTION DEVICE, AND INSPECTION METHOD

Номер: WO2016207986A1
Автор: YOKOI Takahide, SHIRAI Masataka
Принадлежит:

A characteristic value of a plurality of biological samples is optically acquired from a specimen, the characteristic value is compared with prior inspection information for the same specimen, and on the basis of the comparative results, an index is calculated for determining whether to carry out a continuation of processing to genetic analysis of the biological sample or to readjust the sample from the same specimen. Thus, in analysis of a single cell or analysis of a small numbered group of cells, the cost of analysis, time for analysis, and effort can be restrained while suitably selecting inspection samples and securing validity, thereby detecting biomarkers with greater reliability.

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Номер записи: 1
30-05-2014 дата публикации

DATA ANALYSIS DEVICE AND DATA ANALYSIS METHOD

Номер: WO2014080447A1
Автор: SHIRAI Masataka, KAMBARA Hideki, TANIGUCHI Kiyomi
Принадлежит:

The present invention has the objective of providing a data analysis device which, even in cases in which exception data caused by experimental error and the like occur, is capable of suitably implementing clustering. A data analysis device according to the present invention, in accordance with a range of experimental errors described by experimental error data, predefines cluster range parameters for relaxing cluster boundaries. In a process of clustering, with regard to exception data not belonging to any cluster, if an area that is further separated by only a distance defined by cluster range parameters from the exception data belongs to any of the clusters, an assessment is made that the exception data belongs to the cluster, and if the exception data is separated by only the distance and still does not belong to any of the clusters, then an assessment is made that the exception data configures an independent cluster.

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Номер записи: 2
18-10-2012 дата публикации

CELL COLLECTION SYSTEM

Номер: WO2012141157A1
Автор: SHIRAI Masataka, TSUNODA Hiroyuki, MATSUNAGA Hiroko, UCHIDA Kenko
Принадлежит:

The present invention provides: a cell collection system which is simple and has high through-put and high reliability; and a cell collection system which has improved cell collection efficiency. In the present invention, one or multiple fine pores are provided on a cell collection plate, one surface of the plate is introduced directly in a petri dish or the like in such a manner that the one surface can contact with a solution containing cells to be collected and, at the same time, a means for obtaining an optical image of the collected cells through the back surface of the plate is provided, whereby the reliability and easiness of the collection of the cells can be improved. Alternatively, only areas adjacent to the fine pores in the cell collection plate are hydrophilized and other areas are made water-repellent, whereby the efficiency of the collection of the cells can be improved.

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Номер записи: 3
03-12-2015 дата публикации

OPTICAL ANALYSIS DEVICE

Номер: WO2015181872A1
Автор: MIKAMI Hideharu, SHIRAI Masataka
Принадлежит:

The present invention acquires spectral data for a CARS spectrum, or the like, of a sample at a high speed while suppressing data quantity. During scanning of irradiation light that is condensed and irradiated onto a sample, the exposure state of a detection unit of a spectroscope for dispersing light emitted from the sample is maintained, and spectral data is acquired in which spectrums occurring in a plurality of positions in the sample have been added.

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Номер записи: 4
07-01-2016 дата публикации

ARRAY DEVICE FOR NUCLEIC ACID ANALYSIS, NUCLEIC ACID ANALYSIS DEVICE, AND NUCLEIC ACID ANALYSIS METHOD

Номер: WO2016001963A1
Автор: SHIRAI Masataka, YOKOI Takahide, KAMBARA Hideki
Принадлежит:

The present invention has an plurality of nucleic acid capturing regions that capture nucleic acid molecules and uses the plurality of nucleic acid capturing regions to analyze a plurality of samples simultaneously. An array device for nucleic acid analysis according to the present invention comprises a base, a plurality of nucleic acid capturing regions that are arranged on at least one main surface of the base and that have a nucleic acid capturing means that captures nucleic acids, and a control means that mutually connects or individuates an upper space part of the plurality of nucleic acid capturing regions.

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Номер записи: 5
23-05-2019 дата публикации

METHOD AND APPARATUS FOR COLLECTING BIOMOLECULES FROM SPECIFIC REGION OF TISSUE SECTION

Номер: WO2019097873A1
Автор: SHIRAI Masataka
Принадлежит:

Provided are a biomolecule analysis apparatus and a biomolecule analysis method, which are capable of analyzing samples of cells or micro-regions in a specified position with a microscope image of a tissue section by collecting biomolecules in a single cell or in a micro-region without wounding peripheral cells.

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Номер записи: 6
20-06-2019 дата публикации

METHOD FOR COMPREHENSIVELY ANALYZING 3' END GENE EXPRESSION OF SINGLE CELL

Номер: WO2019116800A1
Автор: TANIGUCHI Kiyomi, SHIRAI Masataka
Принадлежит:

The present invention provides a method for effectively performing a comprehensive gene expression analysis on a single cell level. Specifically, the present invention relates to a method for analyzing a gene expression of a cell using a device with a plurality of micro reaction tanks, wherein the micro reaction tanks are each charged with at least one solid carrier on which a probe including an amplification primer sequence, a cell identification sequence, a molecule identification sequence, and an oligo (dT) sequence is immobilized, the method comprising: a step for introducing a plurality of cells to the micro reaction tanks such that a single cell corresponds to one micro reaction tank; a step for capturing, to the probe, an mRNA derived from the single cell; a step for synthesizing a 1st cDNA by reverse transcription of the captured mRNA, and constructing, on the solid carrier, a 1st cDNA library derived from a single cell; a step for cleaning the solid carrier; a step for synthesizing ...

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Номер записи: 7
08-06-2017 дата публикации

CELL ANALYSIS DEVICE, APPARATUS, AND CELL ANALYSIS METHOD USING SAME

Номер: WO2017094101A1
Автор: SHIRAI Masataka, SAKAI Tomoyuki, UCHIDA Kenko
Принадлежит:

The purpose of the present invention is to provide a single-cell analysis device wherein enhanced nucleic acid capture efficiency and enhanced cell capture efficiency are both achieved, and high-precision single-cell analysis data are thereby obtained. The present invention relates to an improvement of a cell analysis device provided with a two-dimensional array chip, provided with: a plurality of cell capture parts capable of capturing one cell in each thereof; and nucleic acid capture parts corresponding to the cell capture parts, the nucleic acid capture parts for capturing a nucleic acid extracted from the cell captured in the corresponding cell capture part.

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Номер записи: 8
11-08-2016 дата публикации

CELL TREATMENT DEVICE AND CELL TREATMENT SYSTEM

Номер: WO2016125254A1
Автор: SAKAI Tomoyuki, SHIRAI Masataka
Принадлежит:

Provided is a technique whereby, in the case of simultaneously analyzing gene expression of multiple cells at a single cell level, the use amount of an expensive reagent can be reduced, a lowering in reaction efficiency due to turbidity caused by reagents mixed together can be suppressed and a device can be effectively washed so as to protect mRNA from contamination with cells other than the target cells. The cell treatment device according to the present invention comprises: a reaction field in which an organism-derived sample is captured; a housing part in which the sample introduced from outside, a reagent or a washing liquor are temporarily stored; a first flow channel which connects the reaction field and the housing part; an opening through which the sample and the reagent are introduced from outside the device; a second flow channel which connects the opening and the housing part; an inlet through which the washing liquor is introduced from outside the device; and a third flow channel ...

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Номер записи: 9
18-09-2014 дата публикации

TWO-DIMENSIONAL CELL ARRAY DEVICE AND APPARATUS FOR GENE QUANTIFICATION AND SEQUENCE ANALYSIS

Номер: WO2014141386A1
Автор: SHIRAI Masataka, KAMBARA Hideki, TANIGUCHI Kiyomi, TANABE Maiko
Принадлежит:

To express a number of genes in a number of cells, it was necessary to separate the cells, extract the genes therefrom, then amplify nucleic acids, and analyze the expression of the genes by sequence analysis. However, separation of cells seriously injures the cells and an expensive system is required therefor. According to the present invention, the expression of genes in individual cells is highly accurately analyzed by: arranging a pair of structures including a cell-trapping section and a nucleic acid-trapping section in a vertical direction to extract individual genes in cells; synthesizing cDNAs in the nucleic acid-trapping section; amplifying nucleic acids; and then analyzing the sequences thereof with a next generation sequencer.

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Номер записи: 10
02-07-2015 дата публикации

BIOMOLECULE ANALYSIS DEVICE

Номер: WO2015097858A1
Автор: SHIRAI Masataka, KAMBARA Hideki, KAJIYAMA Tomoharu
Принадлежит:

The purpose of the present invention is to provide a biomolecule analysis device capable of collecting biomolecules from within a single cell and analyzing the biomolecules without harming surrounding cells. To solve this problem, a biomolecule analysis device according to the present invention is provided with a means for taking an optical image of a plurality of cells, a means for destroying a portion or the entirety of one or more cells from among the plurality of cells, an array device in which areas for capturing biomolecules released from within cells as a result of the destruction means are arrayed, and a means for associating the portion of the optical image corresponding to the cells destroyed by the destruction means with an area of the array device that captured the biomolecules.

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Номер записи: 11
07-09-2018 дата публикации

SINGLE CELL ANALYZER WITH WASHING FUNCTION

Номер: WO2018159008A1
Автор: KAWABE Shunsuke, SHIRAI Masataka, SAKAI Tomoyuki
Принадлежит:

Provided are: a single cell analyzer, which comprises a substrate, a plurality of cell trapping holes formed in one surface of the substrate and nucleic acid trapping bodies for trapping nucleic acids extracted from individual cells respectively trapped by the single cell trapping holes, characterized in that a nucleic acid trapping area disposed in the vicinity of the cell trapping holes and second holes formed in one surface of the substrate are provided, and the second holes are larger than the cell trapping holes; and a system provided with the single cell analyzer.

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Номер записи: 12
06-04-2017 дата публикации

ANALYSIS METHOD AND DEVICE FOR ANALYSIS

Номер: WO2017056251A1
Автор: YOKOI Takahide, SHIRAI Masataka
Принадлежит:

The present invention relates to a method for standardizing measured expression levels of individual genes in single cell gene expression analysis wherein cells are individually measured in a same container. Specifically, an analysis method that comprises separating cells employed as a sample into individual cells, capturing a biological substance in the individual cells respectively in capture areas and measuring a physical amount caused by the captured biological substance, said method involving a standardization step for capturing a known substance, which serves as a reference for the standardization of the physical amount, in the capture areas prior to the separation of the cells.

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Номер записи: 13
04-06-2015 дата публикации

LIGHT MEASURING DEVICE AND LIGHT MEASURING METHOD

Номер: WO2015079786A1
Автор: SHIOZAWA Manabu, WATANABE Koichi, SHIRAI Masataka, OSAWA Kentaro
Принадлежит:

Reflection-type Coherent anti-Stokes Ramen scattering (CARS) is preferable for surface state measurement due to its signal strength, but it is difficult to identify a surface position because the topographical information is not obtained. The reflection-type CARS microscopy and a high resolution phase sensor are combined. The phase sensor identifies the surface position, and reflected CARS derived from the surface is detected to perform compositional analysis.

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Номер записи: 14
05-10-2017 дата публикации

SYSTEM FOR CAPTURING SINGLE CELL-DERIVED BIOMOLECULES

Номер: WO2017168493A1
Автор: SAKAI Tomoyuki, SHIRAI Masataka
Принадлежит:

Provided is a system for capturing biomolecules in a single cell, said system comprising: a two-dimensional array provided with a plurality of single cell-capturing holes, which are formed on one surface of a substrate, and a biomolecule-capturing area, in which biomolecule-capturing members capturing biomolecules extracted from individual cells respectively captured by the single cell-capturing holes are disposed inside the substrate; a flow channel for flowing a sample, said sample containing a plurality of cells to be measured, from a first direction being parallel to the one surface of the substrate; structures which are formed on the surface in the downstream side of the single cell-capturing holes along the first direction in such a manner as to face the first direction; a first application means, which applies the first flow, and a first control means; and a second application means, which applies a second flow, said second flow being perpendicular to the one surface of the substrate ...

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Номер записи: 15
16-01-2020 дата публикации

CELL ANALYSIS METHOD

Номер: WO2020012816A1
Автор: KAWABE Shunsuke, SAKAI Tomoyuki, SHIRAI Masataka
Принадлежит:

To highly efficiently analyze cell data without increasing cost. A cell analysis method, wherein a single cell analyzer 11 provided with an analysis chip and a membrane filter laminated on the analysis chip is used, comprises: a step for introducing a first liquid containing a cell into the analysis chip; a step for introducing a second liquid having a smaller surface tension than the first liquid into the analysis chip; a step for sucking the first and second liquids with a liquid feeding pump connected to a flow channel on the membrane filter side; a step for introducing a reaction reagent capable of reacting with the cell into the analysis chip and reacting a molecule extracted from the cell with a solid phase; a step for sucking the reaction reagent with a liquid feeding pump; and a step for taking out the solid phase from the analysis chip and analyzing the same.

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Номер записи: 16
30-04-2015 дата публикации

GENETIC ANALYSIS SYSTEM

Номер: WO2015059741A1
Автор: TANABE Maiko, KAMBARA Hideki, SHIRAI Masataka
Принадлежит:

In order to decode selected sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduce a sequence, which differs for each cell, in the respective fragments. But in conventional configurations for analyzing large numbers of cells, there was the problem that the cut fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present system is constructed to also comprise a means, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.

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Номер записи: 17
15-09-2016 дата публикации

OPTICAL MEASUREMENT DEVICE AND OPTICAL MEASUREMENT METHOD

Номер: WO2016143084A1
Автор: SHIOZAWA Manabu, WATANABE Koichi, SHIRAI Masataka
Принадлежит:

In multicolor CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.

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Номер записи: 18
06-02-2014 дата публикации

TAG-SEQUENCE-ATTACHED TWO-DIMENSIONAL CDNA LIBRARY DEVICE, AND GENE EXPRESSION ANALYSIS METHOD AND GENE EXPRESSION ANALYSIS APPARATUS EACH UTILIZING SAME

Номер: WO2014020657A1
Автор: SHIRAI Masataka, KAMBARA Hideki, TANIGUCHI Kiyomi, TANABE Maiko
Принадлежит:

The present invention relates to a method, a device and an apparatus all of which can be used for analyzing the expression of a gene in a cell. Specifically, the present invention relates to: a device for analyzing the expression of a gene, said device being characterized by being equipped with a support, wherein a nucleic acid probe is immobilized in a two-dimensionally distributed state on the surface of the support or at a position adjacent to the surface of the support, and the nucleic acid probe comprises a sequence for capturing a nucleic acid to be tested, a tag sequence for cell recognition purposes which has a known sequence, and a common primer sequence having a known sequence, and wherein different types of the tag sequences are used at different positions in the support, i.e., on the surface of the support and at a position adjacent to the surface of the support; and a method and an apparatus, in each of which the device for analyzing the expression of a gene is used.

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Номер записи: 19
17-03-2016 дата публикации

CELL ANALYSIS SYSTEM AND CELL ANALYSIS METHOD USING SAME

Номер: WO2016038670A1
Автор: SHIRAI Masataka, KAMBARA Hideki, ARIKAWA Kohji
Принадлежит:

The purpose of the present invention is to provide a cell analysis system whereby a comprehensive gene analysis of a cell population and a specific gene analysis of a single cell contained in the population can be carried out at the same time. The cell analysis system according to the present invention is provided with an array device, said array device comprising a plurality of cell traps each capable of capturing a single cell and nucleic acid traps each capturing a nucleic acid extracted from a cell trapped by a cell trap, and a reagent addition unit for adding a reagent to the array device, wherein a nucleic acid trap contains an immobilized first probe for capturing a nucleic acid and the reagent addition unit supplies at least a second probe and a third probe to the array device.

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Номер записи: 20
13-10-2016 дата публикации

GENE ANALYSIS SYSTEM

Номер: WO2016162997A1
Автор: TANABE Maiko, SHIRAI Masataka, SAKAI Tomoyuki
Принадлежит:

In order to interpret an arbitrary sequence region in many genes contained in many cells, it is needed to digest a nucleic acid into fragments and then introduce a sequence that is different from one cell to another cell into each of the fragments. However, in the conventional constitutions for analyzing many cells, there is a problem that the contamination by digested fragments among zones may occur before the introduction of a tag sequence inherent in each of the zones. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of multiple zones on a substrate and then synthesizing complementary DNA (cDNA) to the nucleic acid in each of the zones, said system being equipped with a means for immediately introducing a tag sequence inherent in each of the zones into a reaction product.

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Номер записи: 21
26-07-2018 дата публикации

DEVICE AND METHOD FOR EXTRACTING MULTIPLE BIOMOLECULES FROM SINGLE CELL

Номер: WO2018134907A1
Автор: SHIRAI Masataka, SAKAI Tomoyuki, TANIGUCHI Kiyomi
Принадлежит:

In order to measure trace amounts of multiple kinds of biomolecules in a single cell, the present invention provides a device and method capable of differentiating biomolecules derived from a single cell and separating and extracting samples with high efficiency. Specifically, the present invention provides a single cell analysis device, which has a cell separating means and a reaction field disposed near or inside said separation means, the single cell analysis device being characterized in that: said reaction field has an area in which there are first carriers for trapping a first biomolecule extracted from a trapped cell and second carriers for trapping a second biomolecule different from the first biomolecule; the first carriers and/or the second carriers are provided with an identification tag sequence of the trapped cell; and the first carriers and the second carriers have different, mutually separable physical characteristics.

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Номер записи: 22
08-03-2018 дата публикации

SYSTEM FOR ANALYZING SINGLE CELL

Номер: WO2018042725A1
Автор: SAKAI Tomoyuki, SHIRAI Masataka
Принадлежит:

A device for trapping nucleic acids in single cells, said device being provided with: a two-dimensional array comprising a substrate, a plurality of single cell-trapping holes formed on one surface of the substrate, and a nucleic acid-trapping area that is provided with, inside the substrate, nucleic acid-trapping bodies for trapping nucleic acids extracted from individual cells respectively trapped by the single cell-trapping holes; a flow channel for discharging a solution from the nucleic acid-trapping area, said flow channel being positioned adjacent to the nucleic acid-trapping area of the substrate; and a cylindrical structure that encloses the plurality of single cell-trapping holes therein, said cylindrical structure being disposed on the substrate when a cell suspension is introduced and taken off after cells are trapped by the single cell-trapping holes.

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Номер записи: 23
11-08-2016 дата публикации

FLOW CELL DEVICE FOR SINGLE CELL ANALYSIS, AND SINGLE CELL ANALYSIS DEVICE

Номер: WO2016125251A1
Автор: SHIRAI Masataka, SAKAI Tomoyuki
Принадлежит:

A flow cell device for single cell analysis is provided with: (1) multiple reaction chambers each of which has at least one cell capture section and at least one nucleic acid capture section which are connected to each other through a flow path; (2) a first liquid flow path which is connected to all of the multiple reaction chambers on the side of a first surface; (3) multiple second liquid flow paths which are connected to the multiple reaction chambers in a one-to-one relationship on the side of the first surface; and (4) a third liquid flow path which is connected to all of the multiple reaction chambers on the side of a second surface which is opposed to the first surface.

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Номер записи: 24
06-04-2017 дата публикации

CELL ANALYZER AND CELL ANALYSIS METHOD USING SAME

Номер: WO2017056274A1
Автор: SHIRAI Masataka, SAKAI Tomoyuki
Принадлежит:

The purpose of the present invention is to improve both nucleic acid trapping efficiency and cell trapping efficiency in a single cell analyzer. The present invention pertains to a single cell analysis device provided with a two-dimensional array chip, said two-dimensional array chip comprising a plurality of cell trapping parts capable of trapping individual cells and nucleic acid trapping parts disposed corresponding to the respective cell trapping parts and trapping nucleic acids extracted from the cells trapped by the cell trapping parts. The device according to the present invention is characterized in that: a solution-holding part is further provided adjacent to the nucleic acid trapping parts of the two-dimensional array chip; the solution-holding part comprises a three-dimensional porous body that is capable of absorbing, by capillary phenomenon, a solution retained in the nucleic acid trapping parts and discharging the thus absorbed solution to the outside by applying negative ...

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Номер записи: 25
29-09-2016 дата публикации

NUCLEIC ACID REACTION DEVICE

Номер: WO2016151719A1
Автор: SHIRAI Masataka, SAKAI Tomoyuki
Принадлежит:

For analyzing the sequence for an arbitrary gene region, it is required to introduce different probes into individual reaction vessels, respectively. However, the supply of regents into individual reaction vessels has a problem that the structure of a device is complicated and therefore the cost for the analysis is increased. In the present invention, individual cells are captured in a cell hold region, probes that are respectively immobilized onto nucleic acid reaction sections in advance are released after the capture of the cells. In this manner, the supply of individual reagents to the nucleic acid reaction sections can be eliminated and it becomes possible to analyze many cells by employing an inexpensive device configuration.

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Номер записи: 26