Method for reducing allergenicity of whey protein by utilizing ozone synergetic enzymolysis technology

The invention discloses a method for reducing the allergenicity of whey protein by utilizing an ozone synergistic enzymolysis technology, which is characterized in that based on an allergen protein structure, targeting protease is used for carrying out targeted damage on allergen epitopes, and the whey protein is treated by utilizing ozone, so that the structure of the whey protein is changed, and the allergenicity of the whey protein is reduced. The alkaline protease is favorable for performing targeted cleavage on the allergen epitopes in the main allergen whey protein of cow milk, so that directional enzymolysis based on the allergen epitopes of the whey protein is realized; detecting the peptide fragment distribution condition, residual epitope information and sensitization change of the processed product by using an SDS electrophoresis technology, liquid chromatography-mass spectrometry, indirect competitive ELISA and a KU812 cell model; it is verified that the structural characteristics ...