Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год

Применить Всего найдено 4. Отображено 4.
05-01-2017 дата публикации

HUMANIZED LACTATE DEHYDROGENASE (LDH) SACCHAROMYCES CEREVISIAE AND CONSTRUCTION METHOD THEREOF

Номер: WO2017000870A1
Принадлежит:

Provided are a humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae and the construction method thereof. In particular, the method of gene homologous recombination is used to knockout the genes PDC1, PDC5 and PDC6 which encode the PDC of Saccharomyces cerevisiae, LDH from humans is expressed in Saccharomyces cerevisiae, and antimycin A is used to block electronic chain transfers, thus making humanized lactic acid fermentation replace ethanol fermentation for maintaining glycolysis. Humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae obtained from the present method can express the lactate dehydrogenase (LDH) of humans. LDHA or LDHC is expressed in strains of Saccharomyces cerevisiae suffering from three deficiencies in pdc1Δ pdc6Δ pdc5Δ. After antimycin A is added, the strains can grow, and the production of lactic acid can be detected.

Подробнее
01-11-2018 дата публикации

ANIMAL CELL STRAIN AND METHOD FOR USE IN PRODUCING GLYCOPROTEIN, GLYCOPROTEIN AND USE THEREOF

Номер: WO2018196796A1
Принадлежит:

Provided are an animal cell strain for use in producing a glycoprotein which uses a high-mannose sugar chain as a main N-glycan structure, a method for use in producing a glycoprotein by using the cell strain, a glycoprotein produced by using the method, and a use thereof. At least two genes from among a Golgi mannosidase gene and an endoplasmic reticulum mannosidase gene of the cell strain are damaged or knocked out.

Подробнее
07-04-2023 дата публикации

Underground surface water depth-keeping and quantitative collector for hydrogeology

Номер: CN115931460A
Принадлежит:

The invention relates to the technical field of collectors, and discloses an underground surface water depth-keeping quantitative collector for hydrogeology, which comprises a first disc, self-locking universal wheels are fixedly arranged at the bottom of the first disc, a connecting rod is fixedly arranged at the bottom of the first disc, the connecting rod is fixedly assembled on an upper power output shaft of a driving motor, and the lower power output shaft of the driving motor is fixedly connected with the first disc. The top of the connecting rod is fixedly connected with a second disc, and a sliding hole is formed in the outer edge of the first disc. A driving motor, a connecting rod and a second disc are matched for use, and meanwhile, a shaft rod, a drill bit, a servo motor, a telescopic hose and a fixing ring are matched for use, so that a worker does not need to carry various devices when going out to collect underground water and surface water; and when the underground water ...

Подробнее
04-04-2023 дата публикации

Precise hydrological monitoring station land-based water sampling device

Номер: CN115901360A
Принадлежит:

The invention relates to the technical field of water body sampling, and discloses a precise hydrological monitoring station land-based water body sampling device, which comprises a chassis, two sides of the chassis are in rolling connection with walking crawler belts, the top of the chassis is rotatably connected with a limiting rod, and the outer edge of the limiting rod is fixedly sleeved with a turntable. A supporting plate is fixedly assembled on the top of the chassis, and a first motor is fixedly arranged on the outer wall of the supporting plate. Through the arrangement of the chassis, the rotating disc, the limiting rod, the gear, the supporting plate, the first motor, the first fixing rod, the third fixing rod, the sampling sleeve, the sampling connector, the drill bit and the limiting ring device, the device not only can collect surface water through the sampling connector during sample collection, but also can flexibly adjust the positions of the sampling connector and the drill ...

Подробнее