Polyacrylates containing primary amino groups

A polymeric material containing primary amino groups is prepared by copolymerizing an acylaminophenoxy acrylate or methacrylate with a hydroxyalkyl ester or an amide of acrylic or methacrylic acid, and treating the resulting copolymeric material with a hydrolytic agent to convert the acylamino groups into primary amino groups. Such monomeric materials may also be copolymerized with a crosslinking agent comprising an ester or an amide containing two or more acryloyl or methacryloyl groups.

Sorbents for saccharides,glycoproteins and polymers,containing saccharides and method of their manufacture

Lectines are covalently bonded on vehicles selected from the group comprising oxidized polysaccharides, oxidized glycosilized hydroxyalkylacrylates or hydroxyalkylmethacrylates, oxidized polyacrylamide and polyvinylalcohol to form sorbents. The vehicles may be oxidized by the action of sodium or potassium periodate. Following the bonding of the lectines, remaining active aldehydic groups in the vehicle may be eliminated by the action of a reductant such as sodium borohydride.

Method for preparation of ion exchangers by alkylation and hydroxyalkylation of polymeric hydrophylic anion exchangers

ABSTRACT OF THE DISCLOSURE The present invention relates to a method for pre-paration of ion exchangers from hydrophilic anion-exchanging gels based on acrylate or methacrylate copolymers prepared by copolymerization of amino-group-containing monomers, selected from a group comprising aminoalkyl acrylates and methacrylates, aminoalkylacrylamides and aminoalkylmethacrylamides or on gels prepared by chemical bonding of amine groups to the hydro-phylic matrix of acrylate or methacrylate copolymers. The me-thod consists in alkylation or hydroxyalkylation of amine groups by alkylating agents, as alkyl halogenides, alkyl p-toluenesul-fonates, alkylsulfates and epoxides. Alkylation with alkyldiazo compounds was used for transformation of the undesired carboxy-lic groups of the ion exchangers. Another modification consists in the reaction of halogenated acrylate or methacrylate gels with polyamines or oligomeric polyethylene imines. These reac-tions lead to ion exchangers with modified chemical nature, dis-sociation constant, exchange capacity, degree of hydrophility, distance of the ionogenous groups from the surface of the matrix or the character of microsurroundings. The resulting ion-exchan-ging materials are suitable above all for the sorption and se-paration of biopolymers.

A method of preparing polymers by means of anionic initiators

Ethylenically unsaturated monomers are polymerized with an anionic initiator by adding the initiator portionwise to a stirred polymerizing mixture throughout the course of reaction and effecting the additions only when the temperature of the reactants falls below that temperature at which the effectiveness of the initiator is decreased to one half in 15 minutes or less under the conditions of the reaction. The reaction may be carried out in bulk or in a diluent, and may be terminated at any desired degree of conversion. Exemplified initiators are tert.-butyl lithium, potassium tert.-butyl alcoholate, lithium tert.-butyl alcoholate, sodium tert.-butyl alcoholate, and triphenylmethyl sodium. Exemplified monomers are methyl methacrylate, butyl methacrylate, methyl acrylate, butyl acrylate and acrylonitrile. The Specification includes a drawing of a suitable reactor.

PROCEDURE FOR MAKING ION EXCHANGERS FROM HYDROPHILA POLYMERA ANION EXCHANGER.

Isolation of biologically active compounds by affinity chromatography

A method for the isolation of biologically active compounds by affinity chromatography comprising forming a sorption complex between a solvent soluble biologically active compound to be isolated and biologically active compound linked to a solid carrier by a covalent bond, said solid carrier being a hydrophillic macroporous copolymer.

Method for preparation of hydrophilic polymeric ion exchanging gels

The invention relates to preparation of synthetic macroporous ion-exchanaging materials by a ternary copolymerization of hydrophilic monomers containing non-ionogenous groups with monomers containing ionogenous groups and cross-linking monomers in an aqueous dispersion in the presence of inert components and suspension stabilizers. Hydroxyalkyl, oligoglycol and polyglycol esters and N-substituted or unsubstituted amides of acrylic and methacrylic acid are used as the hydrophilic monomers; compounds CH 2 ═C(R)COX, where R = H or CH 3 , X = --OR 1 N(R 2 )R 3 , --OR 1 SO 3 H, --NHR 1 N(R 2 )R 3 or --HNR 1 SO 3 H, R 1 = alkylene and R 2 and R 3 = H, alkyl, hydroxyalkyl or aminoalkyl, acrylic and methacrylic acid serve as the ionogenous monomers; the crosslinking agents are selected from a group of monomers consisting of alkylene, oligoglycol or polyglycol diacrylates and dimethacrylates, alkylenebisacrylamides, alkylenebismethacrylamides, divinylbenzene and other compounds containing more than 2 polymerizable acryloyl or methacryloyl groups. The invented materials are suitable above all for isolation and chromatographic separation of sensitive compounds of the biological origin.

Patent DE2858715C2

METHOD OF ISOLATING AND CLEANING UROKINASE

Die Erfindung beschreibt ein einfaches Chromatographieverfahren zur Gewinnung und Reinigung des Plasminogenaktivators Urokinase. Erfindungsgemaess werden urokinasehaltige Loesungen bei schwach saurem bisneutralem p H-Wert mit neuen Cyanoalkylgruppen enthaltenden Traegern vom Methacrylattyp in Kontakt gebracht. Dabei wird die Urokinase durch Adsorption an die Traeger aus den Loesungen abgetrennt. Durch Desorption von den Traegern mit Loesungen alkalischen p H-Wertes, Dialyse und Lyophilisation wird die Urokinase in reiner Form erhalten. The invention describes a simple chromatography method for obtaining and purifying the plasminogen activator urokinase. According to the invention, urokinase-containing solutions are brought into contact with novel cyanoalkyl group-containing methacrylate-type carriers at a weakly acidic bisneutral p H value. The urokinase is separated from the solutions by adsorption to the carriers. By desorption of the carriers with solutions of alkaline pH value, dialysis and lyophilization, the urokinase is obtained in pure form.

Process for the production of polymers with anion initiators

Preparation of polymers containing aromatic amino groups

Method for preparation of amphoteric ion-exchangers by substitution of hydrophilic polymers

ABSTRACT OF THE DISCLOSURE : The present invention relates to a method for prepara-tion of cation active and amphoteric ion-exchangers from macroporous hydrophilic polymers containing crosslinked copolymers having hydroxyl groups in their structure, said crosslinked copolymers being prepared by suspension copoly-merization of monomers selected from the group consisting of hydroxylalkyl methacrylates, hydroxyalkyl acrylates, oligo-glycol acrylates and oligoglycol methacrylates, with cross-linking divinyl monomers selected from the group consisting of divinylbenzene, ethylene dimethacrylate, butylenediol dia-crylate, said copolymers being modified by treatment with mineral acids or addition compounds of sulfur trioxide, sulfuric acid or chlorosulfuric acid with alcohols or pyridine at a temperature of 0° -50°C for 0,1 to 40 hours, or by the reaction with unsaturated compounds selected among acryloni-trile, methacrylonitrile, sodium vinylsulfonate, in a strongly alkaline medium, or by the reaction with halogenoalkylam-monium compounds.

Method for the production of bead dextran materials for gel chromatography

ABSTRACT OF THE DISCLOSURE: A method for the production of bead dextran materials for gel chromatography, wherein dextran is, be-fore or during the suspension process or in both stages, subjected to reduction in an alkaline solution, advanta-geously by the action of sodium borohydride. The density of crosslinking is controlled above all by variation of the concentration of the initial dextran solution and the re-sulting gel is optionally subjected to the additional crosslinking in a separate subsequent operation. The pro-cedure according to the invention provides the product of higher quality with a lower content of carboxylic groups and substantially facilitates the preparation of gels with a very high and a very low crosslink density.

Methacrylate gel method for medification and application of hydroxyalkyl

Storage container of samples for analysis

ABSTRACT OF THE DISCLOSURE: A storage container of samples for analysis which consists of a cylindric tube and two fittings adapted for connection with a syringe, mutual connection of containers in series, or closing is manufactured from a suitable plastic or glass. A column of sorbent is closed at both ends with a porous partition, screen or a layer of silicate or glass wool. The size of sorbent particles is 20-150 µm. The content of storage container is protected during storage and/or transportation by closures from both sides. The sorbents which are packed into the storage container are selected from silica gel or organic copolymeric carriers of specific or nonspecific functional groups, which are purposefully chosen. The storage container of samples for analysis according to the invention finds the application in general and clinical analyses, toxicology, environmental protection, agriculture, food industry, biology and biotechnologies for entrapping, storage, preparation and processing of real samples after withdrawing from a source and before the proper analytical determination. The design of the storage container of samples substantially reduces time of sample processing at users and also substantially reduces demands for material and labour in manufacturing.

Hydrophilic macroporous three dimensional copolymers of hydroxyalkyl acrylates or methacrylates with crosslinking agents and the method of their manufacturing

The invention pertains to hydrophilic macroporous three dimensional copolymers of hydroxyalkyl methacrylates or hydroxyalkyl acrylates (C 1 -C 4 hydroxyalkyl) with crosslinking monomers which contain covalently attached saccharides via an O-glycosidic bond. The invention further pertains to the method of manufacturing of these copolymers by the direct reaction of the basic three dimensional copolymer containing hydroxyl groups with saccharides or their derivatives in an inert organic solvent under catalysis of HCl, BF 3 , or their mixtures at 20°-100° C. Saccharides and their derivatives are selected from the group comprising monosaccharides, oligosaccharides, deoxy sugars, amino saccharides, acylated saccharides, ether or halogen derivatives of monosaccharides and oligosaccharides, and the like. The invention also pertains to the application of these macroporous three dimensional copolymers with saccharides as sorbents and materials for the affinity chromatography for isolation of physiologically effective materials which contain free bonding sites for saccharides and are able to form specific complexes with saccharides.

Preparation of hydrophilic material for gel chromatography

This invention relates to a method for preparing globular, hydrophilic copolymers which have reticulated internal structures and are suitable for gas and gel chromatography. The method comprises polymerization by suspension polymerization methods of hydrophilic and hydrophilic monomers in aqueous medium in the presence of a component which prevents transfer of the hydrophilic monomer into the aqueous medium and the solubility of the aqueous medium in the monomeric phase.

Process for the isolation of physiologic and biocatalytic effective compounds

Insoluble polymeric carriers for biologically active compounds

Method for producing high-active biologically efficient compounds immobilized on a carrier

The principles of invention consists in a two-step process of immobilization of biologically efficient compounds where the biologically efficient compounds are bonded to a macroporous polymeric carrier containing reactive functional groups from an aqueous solution by a hydrolytically stable linkage. The process is characterized in the first step by a hydrophobic adsorption of the biologically efficient compounds on a macroporous matrix of the carrier and to an increase of their concentration on the solid surface. In the second step, the reaction takes place with the reactive functional groups of carrier and a covalent linkage is formed. The hydrophobic adsorption of compounds determined for immobilization is achieved by addition of inorganic salts in concentration 0.5 to 3.0 mol/1. <??>The invention can be utilized in the preparation of enzymatic catalysts for transformations of compounds and in the preparation of specific sorbents for both the analytical and preparative affinity chromatography. These methods are used in biotechnology, chemical and clinical analysis, food industry, pharmacology and clinical therapy.

PROCESS FOR THE PRODUCTION OF POLYMERS WITH AN AROMATIC AMINO GROUP IN THE SIDE CHAIN

Patent CH622716A5

PROCESS FOR MANUFACTURING INSOLUBLE BIOLOGICALLY ACTIVE SYSTEMS

Column for liquid chromatography

1. A liquid chromatography column comprising a glass tube (1) for the sorbent package having a liquid-permeable partition wall (3) and closures at both ends, a holding means (4) provided around the glass tube (1), and terminal parts adapted for disassembly and provided at both ends of the glass tube (1) and connected to the holding means (4), said terminal parts having each a head part (5) and a front part (2) with an opening (11) for introducing and discharging the liquid which is sealed against the glass tube (1), characterized in that at the ends of the glass tube (1) between the inner wall of the holding means (4) and the outer wall of the glass tube (1) there is provided a sleeve (10) extending over the front side of the glass tube (1) and having on the front side an opening with a seal (7) bearing against the front part (2).

Storage container of samples for analysis

A storage container of samples for analysis which consists of a cylindric tube and two fittings adapted for connection with a syringe, mutual connection of containers in series, or closing is manufactured from a suitable plastic or glass. A column of sorbent is closed at both ends with a porous partition, screen or a layer of silicate or glass wool. The size of sorbent particles is 20-150 um. The content of storage container is protected during storage and/or transportation by closures from both sides. The sorbents which are packed into the storage container are selected from silica gel or organic copolymeric carriers of specific or nonspecific functional groups, which are purposefully chosen.The storage container of samples for analysis according to the invention finds the application in general and clinical analyses, toxicology, environmental protection, agriculture, food industry, biology and biotechnologies for entrapping, storage, preparation and processing of real samples after withdrawing from a source and before the proper analytical determination. The design of the storage container of samples substantially reduces time of sample processing at users and also substantially reduces demands for material and labour in manufacturing.

Carriers for biologically active compounds and methods for the production thereof

There is disclosed a method for the production of carriers for biologically active compounds comprising forming a bond by admixing (a) a three dimensional water insoluble diazotizable polymer operative to function as a primary carrier and (b) a water soluble linear polymer having ligands operative to bind said biologically active compounds and operative to function as a secondary carrier, whereby the union of (a) and (b) is operative to serve as a carrier for biologically active compounds and carriers produced in accordance therewith.

PROCESS FOR THE PRODUCTION OF POLYMERS WITH AN AROMATIC AMINO GROUP IN THE SIDE CHAIN

Column for liquid chromatography

Method for isolation of biologically active compounds by the affinity chromatography

Method for producing high-active biologically efficient compounds immobilized on a carrier

The principles of invention consists in a two-step process of immobilization of biologically efficient compounds where the biologically efficient compounds are bonded to a macroporous polymeric carrier containing reactive functional groups from an aqueous solution by a hydrolytically stable linkage. The process is characterized in the first step by a hydrophobic adsorption of the biologically efficient compounds on a macroporous matrix of the carrier and to an increase of their concentration on the solid surface. In the second step, the reaction takes place with the reactive functional groups of carrier and a covalent linkage is formed. The hydrophobic adsorption of compounds determined for immobilization is achieved by addition of inorganic salts in concentration 0.5 to 3.0 mol/1. <??>The invention can be utilized in the preparation of enzymatic catalysts for transformations of compounds and in the preparation of specific sorbents for both the analytical and preparative affinity chromatography. These methods are used in biotechnology, chemical and clinical analysis, food industry, pharmacology and clinical therapy.

Method of preparation of hydrophilic homogeneous macroporous annexes

Method of preparation of the amphoteric ions with the hydrophilic polymer matrix

1493986 Preparation of amphoteric ion exchangers CESKOSLOVENSKA AKADEMIE VED 12 Feb 1975 [12 Feb 1974] 5984/75 Heading C3P An amphoteric ion exchanger is prepared by reacting a non-ionic polymer gel which is a crosslinked polymer of one or more of an acrylate, methacrylate, acrylamide or methacrylamide, and which contains -OH groups, with a compound which contains two or more reactive groups per molecule and then reacting the reactive groups remaining after said reaction with a compound or mixture of compounds which is or are capable of forming anions and cations by dissociation, or ions in which a positive and a negative site are present in the same group. The compound containing two or more reactive groups per molecule is preferably cyanogen bromide, epichlorohydrin, a diepoxide, a polyepoxide, a diisocyanate or a polyisocyanate. Preferred anion and cation forming compounds and mixtures are an amino acid, a diamine and an amino acid or hydroxyacid, or a dicarboxylic acid and a diamine. In the examples, copolymers of 2-hydroxyethyl methacrylate and ethylene glycol diacrylate; 2- hydroxyethyl acrylate and methylene-bis-acrylamide; 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate; and diethylene glycol mono methacrylate and ethylene glycol diacrylate; are reacted with (a) epichlorohydrin and arginine; (b) cyanogen bromide and arginine; (c) diepoxybutane and arginine; (d) hexamethylene diisocyanate and arginine; (e) diepoxybutane and taurine and (f) diepoxybutane and epsilon amino caproic acid.

Method of filling the stabile chromatographic columns for the highly efficient liquid chromatography by the organic microporous sorbents

Zpusob deleni oligosacharidu kapalinovou chromatografii

Způsob přípravy hydrofilních makroporézních ionexů

Způsob přípravy amfoterních ionexů

Method of hydrogen peroxide's and oxidase substrates' quantitative luminometric determination

Polymeric alkoxides based on hydroxyalkyl methacrylates and a method for their preparation

Verfahren zur herstellung von merkurierten polymeren

Verfahren zur herstellung von merkurierten polymeren

Method of preparing mercurated polymers

Method for preparation of mercurated polymers

Verfahren zur herstellung hydrophiler materialien

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Verfahren zur herstellung von durch chemische modifizierung aktivierten polymeren tragern fur die gelenkte polypeptidsynthese

Polymeric carrier for a controlled synthesis of peptides and the method of preparation

Polymeric carrier for a controlled synthesis of peptides and the method of its preparation

Způsob dělení oligosacharidů kapalinovou chromatograflí

Způsob dělení oligosacharidů kapalinovou cjiromatografií, vyznačující se tím, že se směs oligosacharidů, která je urěena k dělení, zavádí na vrchol sloupce silikagelu s měrným objemem pórů 1,0 až22,0 ml/g, měrným povrchem 350 až 500 nr/g, středním objemem pórů 10 až 20 nm, který obsahuje koválentně váženou alkaníckou stacionární fázi s obsahem uhlíku alespoň 20 % hmotnostních s alkylem obsahujícím 12 až 24 atomů uhlíku s methylovenými zbytkovými silanolovými skupinami, přičemž se.sloupec eluuje vodou při teplotě 20 až 80 C. Způsobu podle vynálezu se používá k dělení oligosacharidů v analytičkám i preparativním měřítku a uplatněním v biotechnologii, zemědělství a v potravinářstvíi

Method of hydrogen peroxide's and oxidase substrates' quantitative fluorometric determination

Organic gel materials cross-linked with chemical cross-links and containing covalently bonded phosphoric acid molecules

Verfahren zur herstellung von dreidimensionalen gelmaterialien mit kovalent gebundener phosphorsaure

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Werkwijze voor het bereiden van dissocieerbare resten van fosforzuur bevattende driedimen- sionale gelachtige materialen.

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Verfahren zur herstellung von dreidimensionalen gelmaterialien mit kovalent gebundener phosphorsaure

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Method for the suspension polymerization and copolymerization of hydrophilic monomers

Verfahren zur Reinigung Alkalialkoholaten

Zasobnik vzorku pro analysu

Způsob kvantitativního fluorimetrického stanovení peroxidu vodíku a substrátů oxidáz

ŘeSení se týká způsobu kvantitativního fluorimetrického stanovení peroxidu vodíku a substrátů oxidáz. Tento způsob spočívá v injekci vzorku substrátu do kontinuálního proudu média. Stykem média s imobilizovaným enzymem odpovádajícím přislužnému substrátu vzniká při enzymatické oxidaci substrátu peroxid vodíku. Ten přichází dále do styku s lmohllizovanou peroxidázou. Médium obsahuje chemickou látku, která v přítomnosti peroxidu vodíku a peroxidázy je schopna vytvořit fluorescenční produkt. Vykazovaná fluorescence Je měřena fluorimetrem. Samotná kvantifikace se provádí na základě porovnáni íluorimetřické odezvy na stanovený vzorek substrátu a odezvy na známá standardní množství zkoumané látky.

Spezifische sorbentien und ihr herstellungsverfahren

Specific sorbents containing bonded d-amino acid ora d-amino acid containing peptide

Methode zur isolierung von proteinen und enzymen

Zpusob pripravy imunologicky funkcnich frakci ze sera inbrednich kmenu mysi

Zpusob vyroby opticky aktivnich alfa aminokyselin z racemickych smesi

Preparation of cation exchangers

Verfahren zur Reinigung Alkalialkoholaten

Verfahren zur herstellung eines mit bromcyan aktivierten, trockenen hydrophilen polymeren traegers biologisch wirksamer verbindungen

Makroporezni polymerni sorbenty a zpusob jejich vyroby

Způsob přípravy imunologicky funkčních frakcí ze séra inbredních kmenu myší

Oligo (iminoethylenove) derivaty polymethakrylatu a zpusob jejich pripravy

Selektives adsorbens zur bindung von immunkomplexen

Die Erfindung beschreibt ein Adsorbens zur selektiven Entfernung von zirkulierenden Immunkomplexen aus Koerperfluessigkeiten durch biospezifische Bindung derselben an das Protein C1q, das an Poly(hydroxyethylmethacrylat)-Copolymere als zellulosehaltige feste Phasen ueber eine Brueckenverbindung immobilisiert ist, wobei die so gebundenen Immunkomplexe durch Abtrennung der festen Phase separiert werden und die feste Phase durch Behandlung mit nicht denaturierenden Medien regeneriert wird.

Oligo{iminoethylenové)dariváty polymethakrylátů a způsob jejich přípravy

Polymery 2-hydroxyetyl-metakrylátu obsahující strukturní jednotky s visícími oligo(iminoetylenovýmí) jednotkami obecného vzorce I, se statistickou distriůbucí obou opakujících se konstitučních jednotek v bočním řetězci, přičemž x:y je 1, obsah dusíku je do 15 %, R=H, CH3, C2H5, C6H5. Tyto polymery se podle vynálezu získají kyselou nebo alkalickou hydrolýzou acylové skupiny v strukturních jednotkách obecného vzorce II, kde η 1 a R má týž význam jako v bodě 1 ta, že se hydrolýzují vodným roztokem minerální kyseliny nebo silné zásady