30-04-2020 дата публикации
Номер: US20200131285A1
Принадлежит:
Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications. 2. The polymer of claim 1 , wherein Ris hydrogen or alkyl.3. The polymer of claim 1 , wherein each Rand Ris hydrogen.4. The polymer of claim 1 , wherein Ris azido claim 1 , amino claim 1 , or Boc-protected amino claim 1 , or combinations thereof.5. The polymer of claim 1 , wherein Lis a methylene linker.9. The polymer of claim 8 , wherein each Rand Ris hydrogen or alkyl; and each Rand Ris hydrogen.10. A substrate having a first surface comprising a polymer of covalently bonded thereto.11. The substrate of claim 10 , further comprising one or more recurring units selected from the group consisting of polyacrylamides claim 10 , polyacrylates claim 10 , polyurethanes claim 10 , polysiloxanes claim 10 , silicones claim 10 , polyacroleins claim 10 , polyphosphazenes claim 10 , polyisocyanates claim 10 , poly-ols claim 10 , and polysaccharides claim 10 , and combinations thereof.12. The substrate of claim 10 , wherein the polymer is covalently bonded to the first surface of the substrate through reaction with a first plurality of functional groups covalently attached thereto the first surface claim 10 , wherein the first plurality of functional groups comprise vinyl claim 10 , acryloyl claim 10 , alkenyl claim 10 , cycloalkenyl claim 10 , heterocycloalkenyl claim 10 , alkynyl claim 10 , cycloalkynyl claim 10 , heterocycloalkynyl claim 10 , nitrene claim 10 , aldehyde claim 10 , hydrazinyl claim 10 , glycidyl ether claim 10 , epoxy claim 10 , carbene claim 10 , isocyanate or maleimide claim 10 , or optionally substituted variants or combinations thereof.13. The substrate of claim 12 , wherein the first plurality of functional groups comprise optionally substituted norbornene claim 12 , glycidyl ether claim 12 , or epoxy claim 12 , or ...
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