Polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

PROBES AND PRIMERS FOR DETECTION OF DENGUE

The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof. 1) Probe consisting of nucleotide sequence set forth as SEQ ID Nos. 1 or 2 , optionally conjugated with detectable labels.2) The probe as claimed in claim 1 , wherein the probe is for detecting dengue infection; and wherein the detectable labels are flurophore at 5′ end and quencher at 3′ end.3) The probe as claimed in claim 2 , wherein the fluorophore is selected from a group comprising fluorescein claim 2 , fluorescein derivatives consisting of 6-Carboxy Fluorescein [FAM] claim 2 , VIC claim 2 , JOE claim 2 , 5-(2′-aminoethyl)aminonaphthalene-1-sulphonic acid claim 2 , coumarin and coumarin derivatives claim 2 , lucifer yellow claim 2 , texas red claim 2 , tetramethylrhodamine claim 2 , tetrachloro-6-carboxyfluoroscein claim 2 , 5-carboxyrhodamine and cyanine dyes claim 2 , preferably 6-Carboxy Fluorescein [FAM]; and the quencher is selected from a group comprising tetra methyl rhodamine claim 2 , 4′-(4-dimethylaminophenylazo)benzoic acid claim 2 , 4-dimethylaminophenyl azophenyl-4′-maleimide claim 2 , tetramethylrhodamine claim 2 , carboxytetramethyl rhodamine and black hole quencher 1 [BHQ] dyes claim 2 , preferably black hole quencher 1 (BHQ1).4) Primer consisting of nucleotide sequence set forth as SEQ ID Nos. 3 or 4.5) The primer as claimed in claim 4 , wherein the primer consisting of the SEQ ID No. 3 is sense primer and the primer consisting of the SEQ ID No. 4 is an antisense primer.6) The primer as claimed in claim 4 , wherein the primers correspond to probe consisting of SEQ ID Nos. 1 or 2; and wherein the primers are used in combination with either the probe ...

Method for Isolation of Nucleic Acids and a Kit Thereof

The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.

Polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris . The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

Polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

A cartridge for purifying a sample and analysis

The present disclosure discloses a cartridge for purifying a sample. The cartridge comprising a first chamber having a plurality of compartments for storing a sample and at least one reagent and mix the a sample with the at least one reagent. A second chamber in fluid communication with the first chamber is configured with a matrix member for matrixing at least one analyte. A third chamber in fluid communication with the second chamber is configured with a waste collection chamber for storing waste fluids matrixed in the second chamber. A fourth chamber in fluid communication with the third chamber, includes at least one tube configured to receive and store the at least one analyte from the second chamber, through the third chamber. The cartridge enables purification of multiple samples in a cycle.

Cartridge for purification of biological samples

The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.

PORTABLE DEVICE FOR PURIFYING BIOLOGICAL SAMPLE AND A METHOD THEREOF

The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample. 1. A portable device for purifying biological sample , the device comprising:a housing;a cartridge holder accommodated in the housing for holding at least one cartridge, the at least one cartridge is configured to purify the biological sample;at least one pump positioned in the housing, the at least one pump is fluidly connectable to the at least one cartridge and is configured to circulate the biological sample and one or more fluids through the at least one cartridge;at least one heating element configured in the housing to selectively heat a matrix chamber in the at least one cartridge; a first actuator coupled to a first valve of one or more valves; and', 'a second actuator coupled to a second valve of the one or more valves; and, 'at least one actuator disposed in the housing and outside the cartridge, wherein the at least one actuator comprises transmit, a first signal to the first actuator to operate the first valve in the at least one cartridge to selectively allow flow of the biological sample and one or more reagent solutions into the matrix chamber;', 'transmit, a second signal to the ...

Probes and Primers for Detection of Malaria

The present disclosure gives description of a method used for the detection and quantification of malarial infection caused either by Plasmodium falciparum or Plasmodium vivax using nucleic acids isolated from blood samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR.

PROBES AND PRIMERS TO DETECT THE TIFOIEA FEVER AGENT

La presente descripción proporciona un método para la detección y cuantificación de la Salmonella que causa la fiebre tifoidea en los seres humanos en las muestras de sangre. Se describe la sonda de oligonucleótidos de la SEQ ID Nº 1, junto con los cebadores de las SEQ ID Nº 2 y 3 para detectar el agente tifoideo que causa infecciones con Salmonella. También proporciona una mezcla de reacción de PCR para la detección de Salmonella que causa la fiebre tifoidea en los seres humanos en las muestras de sangre y un kit para detectar el agente de fiebre tifoidea.Reivindicación 1: Sonda caracterizado porque tiene la secuencia de nucleótidos expuesta como la SEQ ID Nº 1, opcionalmente conjugada con marcas detectables. The present description provides a method for the detection and quantification of Salmonella that causes typhoid fever in humans in blood samples. The oligonucleotide probe of SEQ ID No. 1, together with the primers of SEQ ID No. 2 and 3 for detecting the typhoid that causes Salmonella infections, is described. It also provides a PCR reaction mixture for the detection of Salmonella that causes typhoid fever in humans in blood samples and a kit for detecting the typhoid fever agent. Claim 1: Probe characterized in that it has the nucleotide sequence exposed such as SEQ ID No. 1, optionally conjugated with detectable marks.

Probe and primer sequences for detection of hepatitis b virus by real-time pcr, pcr reaction mixture and kits thereof.

The present disclosure relates to method of determining presence and quantification of Hepatitis B virus (HBV) nucleic acids in biological samples. The present disclosure also discloses probe and primer sequences set forth as SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 for the detection of Hepatitis B Virus. Further, the disclosure provides for PCR reaction mixture and kit for said detection and optional quantification.

A polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris . The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

A polynucleotide and polypeptide sequence and methods thereof

A polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris . The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

A polynucleotide and polypeptide sequence and methods thereof

A polynucleotide and polypeptide sequence and methods thereof

The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris . The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.

Nucleotide sequences, reaction mixture, method and kit thereof

A cartridge for purifying a sample and analysis

The present disclosure discloses a cartridge for purifying a sample. The cartridge comprising a first chamber having a plurality of compartments for storing a sample and at least one reagent and mix the a sample with the at least one reagent. A second chamber in fluid communication with the first chamber is configured with a matrix member for matrixing at least one analyte. A third chamber in fluid communication with the second chamber is configured with a waste collection chamber for storing waste fluids matrixed in the second chamber. A fourth chamber in fluid communication with the third chamber, includes at least one tube configured to receive and store the at least one analyte from the second chamber, through the third chamber. The cartridge enables purification of multiple samples in a cycle.