Microfluidic Device

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network. The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network. The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid. 2. The microfluidic apparatus of claim 1 , further comprising a first substance liquid drain claim 1 , wherein the first substance liquid drain is closed in the first position and wherein the first substance liquid drain is open in the second position.3. The microfluidic apparatus of claim 1 , wherein the substance liquid channel network comprises a first substance liquid inlet claim 1 , at least one second substance liquid inlet and at least one substance liquid combining area.4. The microfluidic apparatus of claim 1 , wherein the substance liquid channel network is filled with a separation liquid comprising an immiscible oil.5. The microfluidic apparatus of wherein the first substance liquid is at least one of an aqueous solution or an organic solvent comprising a first substance.6. A method for providing a coding sequence of droplets claim 1 , wherein individual ones of the sequence of droplets are located between a continuous separation liquid claim 1 , the method comprising:providing a first substance liquid and at least a second substance liquid to a microfluidic channel at a droplet generation junction with the continuous separation liquid, andselectively forming at the droplet generation junction droplets of the first substance liquid and of the at least one second substance liquid to generate a predetermined sequence of droplets; wherein the predetermined sequence of droplets is used as a coding.7. The method of claim 6 , wherein at least one of the first substance ...

Microfluidic Device

An apparatus and a method for providing one or more substance liquids to a microfluidic channel network (). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid. 1. A method for providing a sequence of droplets of one or more substance liquids between a separation liquid , the method comprising:providing a separation liquid to a separation liquid channel;providing at least one of a first substance liquid and at least one second substance liquid to a combination area in the substance liquid channel network to obtain a combined substance liquid; andgenerating sections of the combined substance liquid separated by the separation liquid in a droplet channel.2. The method for providing a sequence of droplets of one or more substance liquids between a separation liquid according to claim 1 , wherein the providing at least one of a first substance liquid and at least one second substance liquid to a mixing area comprises supplying the first substance liquid continuously to a first substance liquid inlet and supplying the at least one second substance liquid continuously to a second substance liquid inlet.3. A method for providing a sequence of droplets of one or more substance liquids between a separation liquid claim 1 , the method comprising:providing a separation liquid to a separation liquid channel;continuously providing at least a first substance liquid to a first substance liquid inlet; andswitching at least one first valve directing the first substance liquid to a droplet generation junction at which droplets are formed, between a first position in which a liquid connection to the droplet generation junction is open and a second position in which the liquid ...

Microfluidic device

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network. The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network. The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Microfluidic Sorting Device

A sorting device () is disclosed comprising a channel () adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction () and a plurality of Braille valves () connected by a plurality of connectors to the sorting junction (). An analysis device (), such as a camera, is used for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples. 1. A sorting device comprising:a channel adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction;a plurality of Braille valves connected by a plurality of connectors to the sorting junction;an analysis device for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples.2. The sorting device of claim 1 , in which the sorting junction and ones of the Braille valves are separated by at least 3000 μm.3. The sorting device of in which the sorting junction and the plurality of Braille valves are on located on two different microfluidic chips connected by tubing.4. The sorting device of claim 1 , in which the fluid is injected by external pumps.5. The sorting device of claim 1 , further comprising a microtiter plate having wells fluidly connected to connections controllable by at least one of the Braille valves.6. The sorting device of claim 1 , wherein at least parts of the sorting device are made of a compressible or expandable polymer.7. The sorting device of claim 1 , wherein the analysis device is one of a camera claim 1 , a microscope or a photomultiplier tube.8. The sorting device of claim 1 , wherein the channel is made of a flexible polymer.9. A method of sorting a plurality of samples in a fluid in a channel comprising:passing the fluid with the plurality of samples along the channel;analysing ...

Cell barcoding in microfluidics

The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.

Microfluidic device

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Microfluidic device

An apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Microfluidic Device for the Generation of Combinatorial Samples

The present disclosure relates to a microfluidic device and a method allowing the generating and screening of combinatorial samples. A microfluidic device for producing droplets of at least one sample into an immiscible phase is provided, the device comprising a droplet maker connecting an immiscible phase channel and a sample channel having at least one sample inlet connected to at least one sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by at least one sample valve, so that the at least one sample flows either towards a sample waste outlet or into the at least one sample inlet channel, wherein different sample inlet channel of the at least one sample inlet channel have the same hydrodynamic resistance resulting from the length, height and width of each sample inlet channel upstream of the droplet maker. 1. A microfluidic device for producing droplets of at least one sample into an immiscible phase , the device comprising:a droplet maker connecting an immiscible phase channel and a sample channel having a plurality of sample inlet connected to a plurality of sample inlet channel injecting the at least one sample into the sample channel, wherein the injection of the at least one sample is controlled by a plurality of sample valves, so that the at least one sample flows either towards a sample waste outlet or into one of the plurality of sample inlet channels, wherein different ones of the plurality of sample inlet channels have the same hydrodynamic resistance resulting from the length, height and width of the sample inlet channel upstream of the droplet maker; and an outlet channel, wherein the hydrodynamic resistance of the outlet channel is lower than the hydrodynamic resistance of the immiscible phase channel.2. The microfluidic device of claim 1 , wherein ones of the plurality of sample inlet channels have a sample fluidic resistor to adjust length.3. The ...

Microfluidic sorting device

A sorting device (10) is disclosed comprising a channel (40) adapted to allow passage of a plurality of samples in a fluid to a first side of a sorting junction (50) and a plurality of Braille valves (105) connected by a plurality of connectors to the sorting junction (50). An analysis device (80), such as a camera, is used for analysing ones of the plurality of the samples and adapted to control ones of the plurality of Braille valves depending on properties of the analysed ones of the plurality of the samples.

Microfluidic Device

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid. 1. A microfluidic apparatus for providing one or more substance liquids to a microfluidic channel network , the microfluidic apparatus comprising at least one first substance liquid inlet , at least one first valve and at least one input device for continuously applying a substantially constant pressure or a substantially continuous flow to the at least one first substance liquid at the at least one first substance liquid inlet wherein the at least one first valve , is switchable between a first position in which a liquid connection to the microfluidic channel network is open and a second position in which the liquid connection to the microfluidic channel network is closed.2401405411415421425431435441445409419429439449. The microfluidic apparatus of claim 1 , further comprising a first substance liquid drain fluidly connectable to the first substance inlet ( claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , ) claim 1 , wherein the substance liquid drain ( claim 1 , claim 1 , claim 1 , claim 1 , ) is closed in a first position and wherein the substance liquid drain is open in a second position.3. The microfluidic apparatus according to claim 1 , further comprising:a separation liquid channel; anda droplet channel;wherein the separation liquid channel is connected to the droplet channel at a droplet generation junction and the droplet generation junction is connected to the microfluidic channel network.4. The microfluidic apparatus ...

CELL BARCODING IN MICROFLUIDICS

The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention. 1. A method for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide or set of components thereof in a microfluidic droplet in a microfluidics system , comprising:(i) feeding a particle comprising DNA and/or RNA into a co-localizing channel,(ii) passing the particle past a series of at least two valve-operated oligonucleotide inlets or sets of valve-operated oligonucleotide inlets, wherein one of the at least two oligonucleotide inlets or sets of oligonucleotide inlets is open while the particle passes it, and wherein each oligonucleotide inlet, when open, feeds a known barcode oligonucleotide or set of components thereof into the co-localizing channel, or each set of oligonucleotide inlets, when open, feeds a set of components of a known barcode oligonucleotide into the co-localizing channel, and(iii) closing the oligonucleotide inlet or set of oligonucleotide inlets that is open after the particle has passed it and opening a different oligonucleotide inlet or set of oligonucleotide inlets of the at least two valve-operated oligonucleotide inlets or sets of valve-operated oligonucleotide inlets,wherein(A) the particle of step (i) and the known barcode oligonucleotide or the set of components thereof of step (ii) are fed into the co-localizing channel while not comprised in microfluidic droplets, and the method further comprises generating microfludic droplets downstream of the at least two valve-operated oligonucleotide inlets or sets of oligonucleotide ...

Microfluidic device for the generation of combinatorial samples

Un dispositivo microfluídico para producir gotitas de al menos una muestra en una fase inmiscible, comprendiendo el dispositivo un formador de gotitas (100) que conecta un canal de fase inmiscible (42) y un canal de muestra que tiene una pluralidad de entradas de muestra (25) conectadas a una pluralidad de canales de entrada de muestra (27) que inyectan la al menos una muestra en el canal de muestra (29), en donde la inyección de la al menos una muestra se controla mediante una pluralidad de válvulas de muestra (20), de manera que la al menos una muestra fluye o bien hacia una salida de residuos de muestra (30) o hacia un canal de la pluralidad de canales de entrada de muestra (27), en donde la pluralidad de canales de entrada de muestra (27) tienen la misma resistencia hidrodinámica resultante de la longitud, la altura y la anchura del canal de entrada de muestra (27) corriente arriba del formador de gotitas (100), el dispositivo microfluídico tiene un canal de salida (60) corriente abajo del formador de gotitas (100), en el que las gotitas fluyen hacia dicho canal de salida, caracterizado por que la resistencia hidrodinámica del canal de salida (60) es menor que la resistencia hidrodinámica del canal de fase inmiscible (42), y en donde dicha resistencia hidrodinámica del canal de salida resultante de la longitud, la altura y la anchura del canal de salida es menor que la resistencia hidrodinámica de los canales de entrada de muestra. A microfluidic device for producing droplets of at least one sample in an immiscible phase, the device comprising a droplet former (100) connecting an immiscible phase channel (42) and a sample channel having a plurality of sample inlets ( 25) connected to a plurality of sample inlet channels (27) that inject the at least one sample into the sample channel (29), wherein the injection of the at least one sample is controlled by a plurality of sample valves (20), such that the at least one sample flows either into a sample waste outlet ...

Cell barcoding in microfluidics

The present invention relates to the field of microfluidics and in particular to methods for co-localizing a particle comprising DNA and/or RNA with a known barcode oligonucleotide. Thereby, the transcriptome or DNA of a cell can be barcoded and correlated to a cell phenotype or examined for the effect of a drug on the cell. The invention also provides microfluidic devices and systems having properties which make them particularly suitable for use in the methods of the invention.

A microfluidic apparatus to combine two substance liquids

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Microfluidic device for generating a sequence of substances in a microfluidic channel

A microfluidic apparatus to combine two substance liquids

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Dispositivo de clasificación microfluídico

Microfluidic sorting device

A microfluidic apparatus to combine two substance liquids

The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network (30). The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network (30). The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.

Automated droplet manipulation in microfluidic systems

Disclosed herein is a method of operating a microfluidic system, a microfluidic system for manipulating sample objects, and a control system for operating a microfluidic system. The method according to the invention is for operating a microfluidic system that comprises a manipulation zone for manipulating sample objects flowing through the manipulation zone, a first detection zone arranged upstream of or in the manipulation zone and a second detection zone arranged upstream of, in or downstream of the manipulation zone. A first count is determined that characterizes a number of sample objects flowing through the first detection zone. Sample objects are manipulated in the manipulation zone. A second count is determined that characterizes a number of sample objects flowing through the second detection zone. One or more manipulation parameters for manipulating the sample objects in the manipulation zone are adjusted based on the first count and the second count.