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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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08-03-2012 дата публикации

Compounds derived from muscodor fungi

Номер: US20120058058A1

Автор: John Kenneth Baird, Jonathan S. Margolis, Jorge I. Jimenez, Sarah F. Lego

Принадлежит: AgraQuest Inc

The present invention relates to novel compounds and compositions and the use of them for the control of fungal and bacterial pathogens, insect pests, acari, nematodes and other invertebrate pests including, but not limited to post-harvest and soil diseases, building mold remediation, and seed and grain sanitation.

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Номер записи: 1

09-08-2012 дата публикации

Method for Seed Devitalization

Номер: US20120204294A1

Автор: Barry W. Schafer, Rod A. Herman

Принадлежит: DOW AGROSCIENCES LLC

The invention provides a method for devitalizing plant seed, the method comprising the steps of hydrating a viable whole plant seed and freezing the hydrated whole plant seed. The invention further provides a collection of devitalized whole plant seed wherein the integrity of genomic DNA and protein within the devitalized plant seed is preserved.

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Номер записи: 2

08-11-2012 дата публикации

Pesticidal compositions comprising 4,5-dihydroxyindan-1-one

Номер: US20120283097A1

Автор: Abraham Sztejnberg, Izhak Bilkis, Uri Gerson, Zahi Paz, Zohar Kerem

Принадлежит: Yissum Research Development Co of Hebrew University of Jerusalem

Provided are pesticidal compositions comprising 4,5-dihydroxyindan-1-one or derivatives thereof for protecting important crops against mites, fungi, and bacteria. The compositions may be manufactured by fractionating fungal extracts.

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Номер записи: 3

31-01-2013 дата публикации

Reduction and induction of protein folding

Номер: US20130025197A1

Автор: Gabriel James Tambunga

Принадлежит: Individual

The Reduction and Induction of Protein Folding is a process where isotopes of atoms that compose a protein are replaced by other isotopes that can either enhance proper or improper protein folding in a living organism. To enhance proper protein folding, atoms or molecules with isotopes with typically the same number of protons and neutrons can be applied to a living organism through a created environment. To enhance improper protein folding, atoms or molecules with isotopes with typically different number of protons and neutrons can be applied to a living organism through a created environment. The isotopes in an atom or molecule can be accumulated and created artificially, respectively, where the living organism consumes the accumulated atoms or created molecules where eventually the living organism will only contain the isotopes or molecules that contain the desired isotopes.

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Номер записи: 4

07-02-2013 дата публикации

Annual brome control using a native fungal seed pathogen

Номер: US20130035231A1

Автор: Julie Beckstead, Susan Elizabeth Meyer, Suzette Clement

Принадлежит: Gonzaga Univ, US Department of Agriculture USDA

Formulations having a selective, mycoherbicide activity for killing ungerminated seeds of invasive grass species are provided. An agricultural, mycoherbicide formulation is taught for killing ungerminated seeds of invasive grass species. The formulations can comprise, for example, a slow-growing strain of Pyrenophora semeniperda , a fast-growing strain of Pyrenophora semeniperda , or a combination thereof, and an agriculturally acceptable carrier. As such, the teachings include a composition comprising a mixture of a slow-growing strain of Pyrenophora semeniperda and a fast-growing strain of Pyrenophora semeniperda . The mixture of strains can be used to provide a mixture of virulence that is useful for killing carryover seed banks, with regard to both type and level of virulence.

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Номер записи: 5

25-04-2013 дата публикации

GENERATION OF PLANTS WITH ALTERED PROTEIN, FIBER, OR OIL CONTENT

Номер: US20130101727A1

Автор: Davies John P., Ng Hein Tsoeng (Medard), Wagner D. Ry

Принадлежит: AGRIGENETICS, INC.

The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an IMQ nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype. 1. A transgenic plant , comprising a plant transformation vector comprising a nucleic acid molecule comprising a nucleotide sequence that encodes an IMQ (Improved Meal Quality) polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 100 , or an IMQ polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 100 , whereby the transgenic plant has an improved meal quality phenotype , relative to control plants.2. The transgenic plant of claim 1 , wherein the IMQ polypeptide comprises the amino acid sequence as set forth in SEQ ID NO: 100.3Brassica. The transgenic plant of claim 1 , which is selected from the group consisting of plants of the species claim 1 , including canola and rapeseed claim 1 , soy claim 1 , corn claim 1 , sunflower claim 1 , cotton claim 1 , cocoa claim 1 , safflower claim 1 , oil palm claim 1 , coconut palm claim 1 , flax claim 1 , castor claim 1 , peanut claim 1 , wheat claim 1 , oat claim 1 , and rice.4. The transgenic plant of claim 3 , wherein the plant is canola.5. The transgenic plant of claim 1 , wherein an improved meal quality phenotype comprises an increase in available metabolizable energy in meal produced from seeds of the transgenic plant claim 1 , relative to control plants.6. The transgenic plant of claim 5 , wherein an increase in available metabolizable energy comprises an altered protein and/or fiber content in the seeds of the transgenic plant.7. The transgenic plant of claim 6 , wherein the protein content is increased and/or the fiber content is decreased.8. The transgenic plant of claim 5 , wherein an increase in available ...

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Номер записи: 6

14-11-2013 дата публикации

Method for Producing a Stress Tolerant Plant or Precursor Thereof

Номер: US20130305415A1

Автор: HADLEY Paul, Lopez Carlos Marcelino Rodriguez, Tricker Penelope, Wilkinson Michael James

Принадлежит: ABERYSTWYTH UNIVERSITY

Provided are methods for the production of a stress tolerant plant or precursor thereof. The methods comprise (i) subjecting one or more parental plants to one or more stress conditions selected from unfavourable conditions relating to relative humidity, water availability, periodic drought, nutrients, sunlight, wind, temperature, pH, exogenous chemicals, chemical toxins such as salt, herbivory, prophylactic chemicals, fertilizers, pathogen attack such as bacterial, fungal, or virus infection and pest infestation; and (ii) generating offspring from said one or more parental plants. The offspring show enhanced tolerance relative to the one or more parental plants to one or more stress conditions selected from unfavourable conditions relating to relative humidity, water availability, periodic drought, nutrients, sunlight, wind, temperature, pH, exogenous chemicals, chemical toxins such as salt, herbivory, prophylactic chemicals, fertilizers, pathogen attack such as bacterial, fungal, or virus infection and pest infestation. Also provided are plants, or precursor thereof, produced by the methods and assays for identifying a plant, or precursor thereof, produced by the methods. 1. A method for the production of a stress tolerant plant or precursor thereof , the method comprising:(i) subjecting one or more parental plants to one or more stress conditions selected from unfavourable conditions relating to relative humidity, water availability, periodic drought, nutrients, sunlight, wind, temperature, pH, exogenous chemicals, chemical toxins including salt, herbivory, prophylactic chemicals, fertilizers, pathogen attack including bacterial, fungal, or virus infection and pest infestation; and(ii) generating offspring from said one or more parental plants,wherein said offspring show enhanced tolerance relative to the one or more parental plants to one or more stress conditions selected from unfavourable conditions relating to relative humidity, water availability, periodic ...

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Номер записи: 7

13-01-2022 дата публикации

METHODS OF CROSSBREEDING FUNGI ORGANISMS

Номер: US20220007604A1

Автор: Tudela Jarred

Принадлежит:

Methods of crossbreeding fungi organisms. The methods include placing a first fungus organism on a first growth medium. The first fungus organism is selected from the genus or . The methods further include placing a second fungus organism on the first growth medium adjacent to the first fungus organism. The second fungus organism is selected from the genus or and is different than the first fungus organism. The methods include allowing the first fungus organism to replicate to form a first colony and allowing the second fungus organism to replicate to form a second colony. The methods further include allowing the first colony and the second colony to expand until they intersect along a clamp line where the first colony and the second colony exchange genetic material between them to yield a crossbred fungus organism. 1. A method of crossbreeding fungi organisms , comprising the steps of:providing a first growth medium;{'i': Psilocybe', 'Panaeolus;, 'placing a first fungus organism on the first growth medium, wherein the first fungus organism is selected from the genus or'}{'i': Psilocybe', 'Panaeolus, 'placing a second fungus organism on the first growth medium adjacent to the first fungus organism, wherein the second fungus organism is selected from the genus or and is different than the first fungus organism;'}allowing the first fungus organism to replicate and to form a first colony on the first growth medium;allowing the second fungus organism to replicate and to form a second colony on the first growth medium; andallowing the first colony and the second colony to expand until they intersect along a clamp line where the first colony and the second colony exchange genetic material between them to yield a crossbred fungus organism.2. The method of claim 1 , further comprising harvesting the crossbred fungus organism by removing the clamp line from the first growth medium.3. The method of claim 2 , further comprising transferring the clamp line to a second growth ...

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Номер записи: 8

13-01-2022 дата публикации

NOVEL CARRIER FLUIDS FOR LIQUID FUNGAL SPORE FORMULATIONS

Номер: US20220007643A1

Автор: EIBEN Ute, Gaertzen Oliver, HEUMANN Roman, HILSCHER Ulrike, SEEMAN Antje, TEMMING Kai

Принадлежит:

The present invention relates to a liquid preparation comprising at least one carboxylic ester as defined and fungal spores and a method for controlling phytopathogenic fungi, insects and/or nematodes in or on a plant, for enhancing growth of a plant or for increasing plant yield or root health comprising applying an effective amount of the liquid preparation or the liquid composition according to the invention to said plant or to a locus where plants are growing or intended to be grown. 1. A liquid preparation comprising at least one carboxylic ester composed of a carboxylic acid moiety and an alcohol moietywherein said carboxylic ester is not a carboxylic acid triglyceride from vegetable oils, and fungal spores of a fungus exerting a beneficial effect on plants,wherein said at least one carboxylic ester containsa) a carboxylic monoacid moiety and a monoalcohol moietyb) at least one carboxylic monoacid moiety and a polyalcohol moiety orc) a carboxylic polyacid moiety and at least one monoalcohol moiety;wherein said monoalcohol moiety is a branched, linear, cyclic, acyclic or partially cyclic, saturated or partially unsaturated C1-C24 monoalcohol moiety;wherein said carboxylic monoacid moiety is a branched, linear, cyclic, acyclic or partially cyclic, saturated or partially unsaturated C2-C24 carboxylic monoacid moiety, optionally carrying at least one OH functionality;wherein said polyalcohol moiety is a branched, linear, cyclic, acyclic or partially cyclic, saturated or partially unsaturated di-, tri-, tetra-, penta- or hexavalent C2-C20 polyalcohol moiety; andwherein said carboxylic polyacid moiety is a branched, linear, cyclic, acyclic or partially cyclic, saturated or partially unsaturated C2-C20 carboxylic polyacid moiety.2. The liquid preparation according to claim 1 , wherein any one of a) claim 1 , b) and/or c) is a mixture of esters comprised of more than one different monoalcohol moiety claim 1 , polyalcohol moiety claim 1 , carboxylic monacid moiety or ...

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Номер записи: 9

10-01-2019 дата публикации

ENDOPHYTE COMPOSITIONS AND METHODS FOR IMPROVEMENT OF PLANT TRAITS IN PLANTS OF AGRONOMIC IMPORTANCE

Номер: US20190008158A1

Автор: von Maltzahn Geoffrey

Принадлежит:

Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes. 1. A method of preparing a synthetic combination , comprising inoculating a plurality of plant elements with a formulation comprising at least a first and a second endophyte population heterologously disposed to the plant elements , wherein the first endophyte comprises a first nucleic acid sequence having at least 97% identity to a nucleic acid sequence selected from column A of a pair of endophytes listed in any of Tables 10-16 and the second endophyte comprises a second nucleic acid sequence having at least 97% identity to a nucleic acid sequence selected from column B of the pair , wherein the endophyte populations are present in the formulation in an amount capable of modulating a trait of agronomic importance in plants comprising or derived from said plant elements , as compared to reference isoline plants not comprising or not derived from plant elements inoculated with said formulation.2. A method of preparing a synthetic combination , comprising inoculating a plurality of seeds with a formulation comprising at least a first and a second endophyte population heterologously disposed to the seeds , wherein the first endophyte comprises a first nucleic acid sequence having at least 97% identity to a nucleic acid sequence selected from column A of a pair of endophytes listed in any of Tables 10-16 and the second endophyte comprises a second nucleic acid sequence having at least 97% identity to a nucleic acid sequence selected from column B of the pair , wherein the endophytes are present in the formulation in an amount capable of modulating a trait of agronomic importance , as compared to reference isoline seeds sowed under the same conditions.3. A method of improving a plant phenotype under stress conditions , comprising inoculating a plurality of plant elements with a ...

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Номер записи: 10

17-02-2022 дата публикации

Artificial Plant Seed and Uses Thereof

Номер: US20220046877A1

Автор: Cogan Noel, Mohamaden Ehab Ibrahim Ahmed, Spangenberg German Carlos

Принадлежит:

The present invention relates generally to an artificial plant seed and to its use for plant propagation, in particular, for the propagation of cannabis plants. 1. An artificial seed comprising isolated meristematic plant tissue encapsulated by a biocompatible polymer , wherein the meristematic plant tissue is derived from a cannabis plant and sterilized prior to encapsulation.2. The artificial seed of claim 1 , wherein the meristematic plant tissue is selected from the group consisting of plant cells claim 1 , callus tissue claim 1 , protoplasts claim 1 , a plant organ claim 1 , a zygotic embryo and a somatic embryo.3. The artificial seed of claim 2 , wherein the plant organ comprises an adventitious shoot claim 2 , a micronodule claim 2 , an axillary bud claim 2 , an apical bud and/or a scion.4. The artificial seed of claim 3 , wherein the plant organ comprises an axillary bud.5. The artificial seed of any one of to claim 3 , wherein the biocompatible polymer is selected from the group consisting of gelatin claim 3 , casein claim 3 , arabinoxylan claim 3 , soluble starch claim 3 , chitin claim 3 , pectin claim 3 , alginate claim 3 , methyl cellulose claim 3 , hydroxyethyl cellulose claim 3 , methylhydroxypropyl cellulose claim 3 , methylhydroxyethyl cellulose claim 3 , hydroxylpropyl cellulose claim 3 , sucrose and mixtures thereof.6. The artificial seed of claim 5 , wherein the biocompatible polymer comprises alginate.7. The artificial seed of claim 6 , wherein the alginate is sodium alginate or calcium alginate.8. The artificial seed of any one of to claim 6 , wherein the biocompatible polymer comprises a polysaccharide.9. The artificial seed of claim 8 , wherein the polysaccharide is selected from the group consisting of sucrose claim 8 , lactose and maltose.10. The artificial seed of claim 9 , wherein the polysaccharide is sucrose.11. The artificial seed of any one of to claim 9 , wherein the meristematic plant tissue is encapsulated by at least two layers of ...

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Номер записи: 11

17-02-2022 дата публикации

Symbiont for enhancement of plant performance

Номер: US20220046929A1

Автор: Kelly Craven, Prasun Ray

Принадлежит: Noble Research Institute LLC

The present disclosure provides a novel endophyte, Serendipita vermifera ssp. bescii (“ S. bescii ”), uses thereof and methods incorporating the use thereof for enhancement of plant performance, particularly the use of S. bescii with phosphite as a phosphorous source. The present disclosure also provides methods for detecting the presence of and identifying S. bescii.

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Номер записи: 12

17-02-2022 дата публикации

METHOD FOR INCREASING STORAGE STABILITY OF FUNGAL SPORES

Номер: US20220046930A1

Автор: LANVER Daniel, Neumann Fred, STEINWENDER Bernhardt Michael, WUTTKE Martina

Принадлежит: Bayer Cropscience Biologics GMBH

The present invention relates to a method of producing fungal spores with prolonged storage stability or of prolonging the storage stability of fungal spores comprising subjecting fungal spores to a drying procedure to result in a residual moisture content of 12% or less and a composition comprising fungal spores obtained by said method. 1. A method of producing fungal spores with prolonged storage stability or of prolonging the storage stability of fungal spores comprising subjecting fungal spores to a drying procedure to result in a residual moisture content of less than 12%.2. The method according to claim 1 , wherein said residual moisture content is between 1.5 wt.-% and 6 wt.-%.3. The method according to claim 1 , wherein the germination rate of said spores after 6 months at room temperature is at least 80%.4. The method according to claim 1 , wherein said drying procedure comprises vacuum drying.5. The method according to claim 4 , wherein said vacuum drying is effected for up to 16 hours.6. The method according to claim 4 , wherein said vacuum drying is effected at a temperature of up to 30° C.7. The method according to wherein said vacuum drying is effected at a pressure of 10-1 mbar.8. The method according to claim 1 , wherein said fungal spores are conidia.9. The method according to claim 1 , wherein said fungal spores are from a fungus having beneficial activity in the area of plant protection.10. The method according to claim 9 , wherein said fungus has fungicidal claim 9 , insecticidal claim 9 , nematicidal or herbicidal activity or supports and/or promotes and/or stimulates plant health and/ or plant growth.11Coniothyrium minitansMicrosphaeropsis ochraceaAspergillus flavusGliocladium roseumTalaromyces flavusAmpelomyces quisqualisGliocladium catenulatumClonostachys roseacatenulateTrichoderma asperellumTrichoderma virideTrichoderma atrovirideCladosporium cladosporioides. The method according to claim 9 , wherein said fungus has fungicidal activity and ...

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Номер записи: 13

31-01-2019 дата публикации

FUNGAL ENDOPHYTE SPECIES

Номер: US20190029268A1

Автор: GUR Yonatan, LLORENS Eugenio, OFEK-LALZAR Maya, SHARON Amir, SHARON Or

Принадлежит: Ramot at Tel-Aviv University Ltd.

A composition of matter comprising an agriculturally acceptable carrier and an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 1-5 or an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 6-10 is disclosed. Uses thereof are also disclosed. 1. A composition of matter comprising an agriculturally acceptable carrier and an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 1-5 or an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 6-10.2. The composition of matter of claim 1 , wherein the endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 1-5 is deposited under the NRRL deposit No. 67223 or wherein said endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 6-10 is deposited under the NRRL deposit No. 67222.34-. (canceled)5. The composition of matter of claim 1 , wherein said endophyte is in the form of a spore claim 1 , a hyphae claim 1 , or a mycelia.6. The composition of matter of claim 1 , further comprising at least one agent which promotes the growth of a plant.78-. (canceled)9. The composition of matter of claim 1 , further comprising a fertilizer.10. The composition of matter of claim 1 , wherein said endophyte is viable.1128-. (canceled)29. A method of enhancing the growth of a plant comprising:(a) inoculating the plant or a part thereof with an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 1-5 or an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs: 6-10; and(b) growing the plant, thereby improving the growth of the plant.30. A method of providing a plant tolerance to a stressful condition comprising:(a) inoculating the plant or a part thereof with an endophyte which expresses polynucleotides having the sequences as set forth in SEQ ID NOs ...

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Номер записи: 14

30-01-2020 дата публикации

INOCULANTS AND METHODS FOR USE THEREOF

Номер: US20200029519A1

Автор: BALLARD Ross Alexander, FRANCO Christopher Milton Mathew, LE Hoang Xuyen

Принадлежит:

The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention. 162.-. (canceled)63StreptomycesStreptomyces. A co-inoculated leguminous plant reproductive material , which is co-inoculated with at least one rhizobial microorganism and at least one microorganism , wherein the at least one microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 97% identical to SEQ ID NO: 4 or 10 , wherein the percentage identity is calculated over the full length of the query sequence.64RhizobiumSinorhizobiumBradyrhizobium. The co-inoculated leguminous plant reproductive material of claim 63 , wherein the rhizobial microorganism is a sp. claim 63 , a sp. or a sp.65StreptomycesStreptomyces. The co-inoculated leguminous plant reproductive material of claim 63 , wherein the leguminous plant reproductive material is a seed coated with a first formulation comprising the at least one microorganism claim 63 , and wherein the first formulation comprises at least about 200 individual cells of the microorganism.66. The co-inoculated leguminous plant reproductive material of claim 65 , wherein the first formulation comprises an additive.67. The co-inoculated leguminous plant reproductive material of claim 66 , wherein the additive is xanthan gum claim 66 , and wherein the amount of xanthan gum in the first formulation is 0.3% w/v.68. The co-inoculated leguminous plant reproductive material of claim 65 , wherein the first formulation further comprises the rhizobial microorganism. ...

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Номер записи: 15

24-02-2022 дата публикации

MATERIALS AND METHODS FOR THE CONTROL OF NEMATODES

Номер: US20220053751A1

Автор: ALIBEK Ken, DIXON Tyler, FARMER Sean, FOTSCH ALEX, MAZUMDER Sharmistha, MILOVANOVIC Maja, ZORNER Paul S.

Принадлежит:

The invention provides materials and method for controlling pests, in particular, nematodes. The invention also provides compositions comprising biosurfactants as pesticides. 1. A method for controlling nematodes wherein said method comprises contacting the nematodes with a microbe-based nematicidal composition that has been prepared by cultivating a microorganism under conditions such that the microorganism produces a biosurfactant , wherein said nematicidal composition comprises the microorganism and/or broth in which the microorganism is cultivated.2. The method claim 1 , according to claim 1 , wherein the biosurfactant is a glycolipid.3. The method claim 2 , according to claim 2 , wherein the glycolipid is a sophorolipid (SLP).4. The method claim 2 , according to claim 2 , wherein the glycolipid is a mannosylerytrithol lipid (MEL).5Starmerella, PichiaPseudozyma.. The method claim 1 , according to claim 1 , wherein the microorganism is a fungus selected from and6Starmerella bombicola.. The method claim 5 , according to claim 5 , wherein the fungus is7Pseudozyma aphidis.. The method claim 5 , according to claim 5 , wherein the fungus is8Pichia anomalaWickerhamomyces anomalus. The method claim 5 , according to claim 5 , wherein the fungus is ().9. The method claim 1 , according to claim 1 , wherein the broth is applied to nematodes claim 1 , and/or their environment claim 1 , without first separating biosurfactant from cell mass.10Meloidogyne incognitalBelonolaimus longicaudatusHeterodera glycinesPratylenchusXiphinemaTylenchulus semipenetrans. The method claim 1 , according to claim 1 , used to control a nematode selected from root-knot nematode () claim 1 , sting nematode () claim 1 , soybean cyst nematode () claim 1 , lesion nematode (sp.) claim 1 , dagger nematode (sp.) claim 1 , and citrus nematode ().11. The method claim 1 , according to claim 1 , wherein said method is used to control nematode pests of plants.12. The method claim 11 , according to claim 11 , ...

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Номер записи: 16

24-02-2022 дата публикации

USE OF CHAETOMIUM GLOBOSUM STRAIN IN CONTROLLING FUSARIUM CROWN ROT OF WHEAT

Номер: US20220053771A1

Автор: Han Zixing, Li Lijuan, Li Yahong, Liu Lulu, Shi Ruijie, Song Yuli, Wang Junmei, Xu Fei, Zhang Jiaojiao

Принадлежит:

The present disclosure provides use of a strain in controlling crown rot (FCR) of wheat. The strain under the deposit designation of 12XP1-2-3 is deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183. Field experiments conclude that coating of wheat seeds with strain 12XP1-2-3 can reduce diseased stem rate, disease rating, and disease index by 19.0%-41.3%, 43.7%, and 4.7%-45.4%, respectively. 1Chaetomium globosumFusarium. Use of a strain in controlling crown rot (FCR) of wheat;{'i': 'Chaetomium globosum', 'wherein, the strain under the deposit designation of 12XP1-2-3 is deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183.'}2Chaetomium globosum. Use of a biocontrol agent comprising the strain according to in controlling FCR of wheat.3Chaetomium globosum. The use according to claim 2 , wherein the biocontrol agent is prepared by: multiplying the strain to obtain a spore suspension claim 2 , and dispersing the spore suspension in a sodium carboxymethyl cellulose solution to obtain the biocontrol agent.4. The use according to claim 3 , wherein the sodium carboxymethyl cellulose solution has a mass fraction of 4% claim 3 , and the spore suspension and the sodium carboxymethyl cellulose solution have a volume ratio of 3:1.5. The use according to claim 2 , wherein wheat seeds are coated at a coating concentration of 5.0-5.3×10ascospores per seed.6. The use according to claim 3 , wherein wheat seeds are coated at a coating concentration of 5.0-5.3×10ascospores per seed.7. The use according to claim 4 , wherein wheat seeds are coated at a coating concentration of 5.0-5.3×10ascospores per seed. This application claims priority to Chinese Patent Application No. 202010832744.5, filed on Aug. 18, 2020. The content of this application is hereby incorporated by reference in its entirety.The present disclosure ...

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Номер записи: 17

24-02-2022 дата публикации

CONIOTHYRIUM MINITANS FOR PROTECTION OF CULTIVATED PLANTS FROM ATTACKS BY FUNGAL PATHOGENS BELONGING TO THE GENUS PHOMA SPP. OR VERTICILLIUM SPP.

Номер: US20220053772A1

Автор: RIHA Karel

Принадлежит:

The present invention relates to a method for controlling phytopathogenic fungi of the genus spp. and spp. comprising applying strain CON/M/91-08 or a mutant having all identifying characteristics of the respective strain in an effective amount to the root system of a plant, to harvest residues or to the soil on which a crop plant grows or is intended to grow. Also disclosed is the use of said strain for controlling phytopathogenic fungi of the genus spp. and spp. 1PhomaVerticilliumConiothyrium minitans. A method for controlling phytopathogenic fungi of the genus spp. and spp. comprising applying strain CON/M/91-08 or a mutant having all identifying characteristics of the respective strain in an effective amount to the root system of a plant , to harvest residues or to the soil on which a crop plant grows or is intended to grow.2Coniothyrium minitans. The method according to claim 1 , wherein said strain is applied in the form of spores claim 1 , conidia claim 1 , hyphae or a combination thereof.3Coniothyrium minitans. The method according to claim 2 , wherein said strain is applied in the form of conidia.4. The method according to claim 3 , wherein said conidia are present in a wettable granule (WG) or a liquid formulation.5. The method according to claim 4 , wherein said formulation is a WG formulation.6Coniothyrium minitans. The method according to claim 1 , wherein said strain is applied pre-planting claim 1 , during growth or post-harvest.7Coniothyrium minitans. The method according to claim 6 , wherein said strain is applied pre-planting.8Brassica. The method according to claim 1 , wherein said plant is the genus spp.9Brassica napus.. The method according to claim 8 , wherein said plant is10Brassica napus. The method according to claim 9 , wherein said plant is winter oilseed rape.11. The method according to claim 1 , further comprising applying simultaneously or subsequently at least one further plant protection agent to the root system of a plant claim 1 , ...

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Номер записи: 18

06-02-2020 дата публикации

INOCULANTS AND METHODS FOR USE THEREOF

Номер: US20200037528A1

Автор: BALLARD Ross Alexander, FRANCO Christopher Milton Mathew, LE Hoang Xuyen

Принадлежит:

The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention. 2Rhizobium. The method of claim 1 , wherein the rhizobial microorganism is a sp.3. The method of claim 1 , wherein the 16S rRNA gene nucleotide sequence is at least 98% identical to SEQ ID NO: 5.4Streptomyces. The method of claim 1 , wherein the co-inoculation comprises coating the seed with a first formulation comprising at least about 200 individual cells of the microorganism.5Streptomyces. The method of claim 4 , wherein the first formulation comprises about 200-2000 individual cells of the microorganism.6. The method of claim 4 , wherein the first formulation comprises an additive.7. The method of claim 6 , wherein the additive is xanthan gum claim 6 , and wherein the amount of xanthan gum in the first formulation is 0.3% w/v.8. The method of claim 4 , wherein the co-inoculating comprises co-inoculating the seed with a second formulation comprising the rhizobial microorganism.9. The method of claim 8 , wherein the second formulation comprises about less than 5×10CFUs of the rhizobial microorganism claim 8 , and wherein the leguminous plant grown from the leguminous seed comprises about 20% more nodules than a control plant grown from a control seed of the same taxon that is singly inoculated with the same amount of the rhizobial microorganism.10. The method of claim 8 , wherein the second formulation comprises about 5×10CFUs of the rhizobial microorganism claim 8 , and wherein a leguminous plant grown ...

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Номер записи: 19

24-02-2022 дата публикации

ISOLATE OF CHAETOMIUM GLOBOSUM AND ITS APPLICATION IN WHEAT GROWTH PROMOTION AND YIELD INCREASE

Номер: US20220056399A1

Автор: FENG Chaohong, Han Zixing, Li Yahong, Song Yuli, Wang Junmei, Xu Fei

Принадлежит:

This invention provides an isolate of , which is numbered as 12XP1-2-3, preserved by China General Microbiological Culture Collection Center with CGMCC No. 17183 on Jan. 23, 2019. This invention also provides an application of in wheat growth promotion and yield increase. The results of field trials showed that by coating wheat seeds with 12XP1-2-3, the chlorophyll content of leaves can be increased by 1.9%-14.7% at booting stage, and the wheat yield can be increased by 2.3%-10.9%. 1Chaetomium globosum. An isolate of , which is numbered as 12XP1-2-3 , and preserved by China General Microbiological Culture Collection Center with CGMCC No. 17183 on Jan. 23 , 2019.2Chaetomium globosum. An application of in wheat growth promotion and yield increase according to .3Chaetomium globosum. A biocontrol agent claim 1 , containing the of .4. The biocontrol agent according to claim 3 , prepared in the following manner:{'i': ['Chaetomium globosum', 'Chaetomium globosum;'], 'sup': '7', '#text': 'the isolate of is inoculated on Potato Sucrose Agar medium and cultured at 25-° C. for 15 days; a spore suspension is obtained by surface washing and coating with a glass spreading rod by adding sterile water, and then filtrated with four layers of sterile gauze; wherein the concentration of spore suspension is 1-2×10ascospores per milliliter, which is a first-degree species containing'}{'i': 'Chaetomium globosum', '#text': 'the spore suspension is inoculated on sterilized wheat grains and cultured for 15 days until grains are covered with hyphae and spores, then a second-degree species containing is obtained after rinsing and isolating; and'}{'i': 'Chaetomium globosum', '#text': 'the biocontrol agent is then obtained by dispersing the second-degree species containing into sodium carboxymethyl cellulose solution.'}5. The biocontrol agent according to claim 4 , wherein the mass fraction of the sodium carboxymethyl cellulose solution is 4%; and the volume ratio of the spore suspension to the ...

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Номер записи: 20

18-02-2021 дата публикации

MODULATED NUTRITIONAL QUALITY TRAITS IN SEEDS

Номер: US20210045390A1

Автор: Bitas Vasileios, Djonovic Slavica, Riley Raymond, Vosnidou Nancy

Принадлежит:

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte. 136.-. (canceled)37Curvularia. A synthetic composition , comprising a soybean plant seed and an endophyte heterologously disposed to the seed , wherein the endophyte is of the genus that is heterologous to the seed and comprises a polynucleotide sequence at least 97% identical to SEQ ID NO: 31 and the endophyte is manually or mechanically disposed to the seed in an amount effective to colonize a soybean plant germinated from the synthetic composition.38. The synthetic composition of claim 37 , wherein a seed obtained from the colonized soybean plant exhibits an increase in crude fat content as compared to a reference seed obtained from a reference soybean plant lacking the heterologous endophyte.39. The synthetic composition of claim 37 , wherein the endophyte comprises a polynucleotide sequence that is 100% identical to SEQ ID NO:31.40Curvularia spicifera. The synthetic composition of claim 37 , wherein the endophyte is a as deposited under NRRL Culture Deposit No. NRRL-67467 claim 37 , or a modified endophyte derived from the deposit.41. (canceled)42. (canceled)43Curvularia. An animal feed or a human food product derived from a seed obtained from a colonized soybean plant grown from a synthetic composition comprising a soybean plant seed and an endophyte heterologously disposed to the seed claim 37 , wherein the endophyte is of the genus that is heterologous to the seed and comprises a polynucleotide sequence at least 97% identical to SEQ ID NO: 31 and the seed obtained from the colonized soybean plant exhibits an increase in crude fat content as compared to a reference seed obtained from a reference soybean plant lacking the heterologous endophyte.4472.-. (canceled) This application is a division of U ...

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Номер записи: 21

18-02-2021 дата публикации

Fungal immunogens and related materials and methods

Номер: US20210046169A1

Автор: Hema P. Narra, Lisa F. Shubitz, Marc J. Orbach

Принадлежит: Arizona Board of Regents of University of Arizona

The present disclosure provides immunogenic materials and methods useful for reducing the risk of fungal infections, particularly valley fever. The disclosure also provides assays for identifying compounds useful to treat valley fever, as well as methods for ameliorating the symptoms of valley fever.

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Номер записи: 22

12-02-2015 дата публикации

Nanobionic engineering of organelles and photosynthetic organisms

Номер: US20150047074A1

Автор: Juan Pablo Giraldo GOMEZ, Markita P. Landry, Michael S. Strano, Sean Mitchell Faltermeier

Принадлежит: Massachusetts Institute of Technology

In one aspect, a composition can include an organelle, and a nanoparticle having a zeta potential of less than −10 mV or greater than 10 mV contained within the organelle. In a preferred embodiment, the organelle can be a chloroplast and the nanoparticle can be a single-walled carbon nanotube associated with a strongly anionic or strongly cationic polymer.

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Номер записи: 23

25-02-2021 дата публикации

ENDOPHYTE COMPOSITIONS AND THE METHODS FOR IMPROVEMENT OF PLANT TRAITS

Номер: US20210051964A1

Автор: Riley Raymond

Принадлежит:

This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes. 1128.-. (canceled)129Periconia. A method of improving a trait of agronomic importance in a plant , comprising heterologously disposing an endophyte to a plant element to produce a treated plant element , wherein the endophyte is a member of the genus and comprises at least one polynucleotide sequence at least 97% identical to SEQ ID NO:32 or SEQ ID NO:33 , and wherein a plant derived from the treated plant element has an improved trait of agronomic importance as compared to a reference plant grown from a reference plant element not further comprising the endophyte.130Triticum. The method of claim 129 , wherein the plant element is of the genus and the improved trait of agronomic importance is selected from the group consisting of germination rate claim 129 , dry shoot biomass claim 129 , and yield.131Glycine. The method of claim 129 , wherein the plant element is of the genus and the improved trait of agronomic importance is selected from the group consisting of root length and root area.132Zea. The method of claim 129 , wherein the plant element is of the genus and the improved trait of agronomic importance is yield.133. The method of claim 129 , wherein the plant element is a seed.134. The method of claim 133 , wherein the seed is modified.135. The method of claim 129 , wherein the endophyte is heterologously disposed in a formulation comprising a stabilizer claim 129 , a preservative claim 129 , a carrier claim 129 , a surfactant claim 129 , an anticomplex agent claim 129 , a fungicide claim 129 , a nematicide claim 129 , a bactericide claim 129 , an insecticide claim 129 , or a herbicide claim 129 , or any combination thereof.136. The method of claim 135 , wherein ...

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Номер записи: 24

22-02-2018 дата публикации

NOVEL METHOD FOR PROMOTING NODULATION IN PLANTS

Номер: US20180049387A1

Автор: Andre Olivier, COMBIER Jean-Philippe

Принадлежит:

Micropeptides (miPEPs), or peptides coded by microRNAs, for promoting nodulation between a plant and a bacterium, as well as their use in this manner. 124-. (canceled)25. A method for promoting nodulation between a plant and a bacterium comprising a step of introducing a miPEP into a plant exogenously , said miPEP also being naturally present in said plant ,said miPEP introduced exogenously being a peptide comprising or consisting of a sequence identical to that of said naturally present miPEP,which naturally present miPEP is a peptide of from 3 to 100 amino acids, of which the sequence is coded by an open reading frame at the 5′ portion of the primary transcript of a miR,said miPEP being capable of modulating the accumulation of said miR in said plant, which miR regulates the expression of at least one gene involved in the nodulation in said plant,the sum of the amount of said miPEP introduced exogenously and of the amount of said naturally present miPEP being strictly greater than the amount of said naturally present miPEP.26. The method according to claim 25 , wherein said miRNA is miR172c27. The method according to claim 25 , wherein said miRNA is miR172c and has a nucleotide sequence consisting of SEQ ID NO: 1.28. The method according to claim 25 , wherein said miPEP is miPEP172c.29. The method according to claim 25 , wherein said miPEP is miPEP172c and has an amino acid sequence consisting of SEQ ID NO: 2.30. The method according to claim 25 , wherein said miRNA is miR167c claim 25 ,31. The method according to claim 25 , wherein said miRNA is miR167c and has a nucleotide sequence consisting of SEQ ID NO: 6.32. The method according to claim 25 , wherein said miPEP is miPEP167c claim 25 ,33. The method according to claim 25 , wherein said miPEP is miPEP167c and has an amino acid sequence consisting of SEQ ID NO: 7.34. The method according to claim 25 , wherein said plant is:{'i': Lotus', 'Glycine max', 'Arachis hypogaea', 'Phaseolus vulgaris', 'Pisum sativum', ' ...

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Номер записи: 25

04-03-2021 дата публикации

CLAY-BASED CARRIER PLATFORM FOR BIOLOGICALS IN AGRICULTURE

Номер: US20210059244A1

Автор: Naik Sajo P., Shinde Shalaka

Принадлежит:

The present invention relates to an improved carrier for sustaining microbes, comprising of a composition having a base material with one or more of a clay or mineral, the composition further including a carbon source and a pH modifier. More specifically, an improved biological carrier platform is provided with scientifically customized formulations that provide hospitable environments for a variety of biologicals, including live microbes and bioactive essential oils. The platform offers an inert granular, mineral base in various grind sizes and forms having specific beneficial effects. In addition, present invention also provides the various processes and methods such as providing an improved carrier comprising a base material having one or more of a clay or mineral, the composition further comprising a carbon source and a pH modifier; and loading a microbial inoculant onto the carrier, wherein the microbial inoculant comprises a plurality of microbes. 1. An improved carrier for sustaining microbes , comprising:a composition comprising a base material comprising one or more of a clay or mineral, the composition further comprising a carbon source and a pH modifier.2. The improved carrier of claim 1 , wherein the base material comprises one or more of montmorillonites claim 1 , calcium montmorillonites claim 1 , attapulgites claim 1 , palygorskites claim 1 , sepiolites claim 1 , kaolins claim 1 , micas claim 1 , zeolites claim 1 , and diatomaceous earth.3. The improved carrier of claim 1 , wherein the carbon source comprises one or more of humic acid claim 1 , leonardite claim 1 , biochar claim 1 , seaweed claim 1 , corn meal claim 1 , millet claim 1 , oats claim 1 , grain starches claim 1 , carboxymethyl celluloses claim 1 , polymers claim 1 , or other organic cellulose carbohydrates.4. The improved carrier of claim 1 , wherein the pH modifier comprises one or more of salts of calcium claim 1 , potassium claim 1 , and sodium.5. The improved carrier of claim 1 , ...

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Номер записи: 26

17-03-2022 дата публикации

METHODS OF CULTIVATING ECTOMYCORRHIZAL FUNGI

Номер: US20220079106A1

Автор: GOLAN Ezekiel

Принадлежит:

A method of inoculating a tree with an ectomycorrhizal fungus is disclosed. The method comprises contacting at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of the at least one non-terrestrial adventitious root by the ectomycorrhizal fungus. Methods of cultivating the ectomycorrhizal fungus are also disclosed as well as systems and devices for same. 1. A method of cultivating an ectomycorrhizal fungus on a tree , comprising growing the ectomycorrhizal fungus on at least one inoculated non-terrestrial adventitious root of the tree , thereby cultivating the ectomycorrhizal fungus on the tree.2. The method of claim 1 , further comprising contacting said at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of said at least one adventitious root by the ectomycorrhizal fungus prior to said growing claim 1 , wherein said growing is effected in a container.3. The method of claim 1 , wherein said tree is at least one meter high.4Tuber, Cantharellus, Boletus, Tricholoma, Hydnum, Lepista, Amanita, Russula, Morchella, Rhizopogon, Craterellus, Lactarius, Polyozellus, Chroogomphus, Laccaria, Albatrellus, Suillus, Amanita, Cortinarius, Gomphus, EntolomaInocybe.. The method of claim 1 , wherein said ectomycorrhizal fungus is an edible ectomycorrhizal fungus of a genus selected from the group consisting of and5. The method of claim 1 , wherein said tree is of the Fagales order.6. The method of claim 2 , wherein said contacting is effected by:(a) planting said non-terrestrial adventitious root in soil adapted for growth of said ectomycorrhizal fungus which comprise an inoculum of said ectomycorrhizal fungus, or(b) planting said non-terrestrial adventitious root in soil adapted for growth of said ectomycorrhizal fungus and inoculating said soil with said ectomycorrhizal fungus.7. The method of claim 6 , wherein said soil ...

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Номер записи: 27

11-03-2021 дата публикации

EPICHLOË ENDOPHYTE

Номер: US20210068364A1

Автор: HUME David Edward, JOHNSON Richard David, SIMPSON Wayne Roydon

Принадлежит:

The present invention relates to isolated strains of endophytes that form symbiotic associations with host plants, wherein the symbiotic associations are combinations of endophytes and host plants that are not found in nature. The invention also relates to combinations of host plants and endophytes that are not found in nature, methods of making such combinations and methods of conferring at least some level of pest resistance on a host plant using such endophytes. 127.-. (canceled)28Epichloë. A combination comprising an isolated strain of endophyte and a non-endogenous host plant , wherein the endophyte comprises a B10 allele size of 188±0.8 base pairs (bp) and a B11 allele size of 112±0.8 bp.29Epichloë. The combination of claim 28 , wherein the endophyte comprises at least one additional SSR allele selected from the group consisting of ans016 claim 28 , ans019 claim 28 , ans033 claim 28 , ans036 claim 28 , egs027 claim 28 , egs031 claim 28 , ces0004 claim 28 , ces0022 claim 28 , ces0041 claim 28 , ces0054 claim 28 , ces0060 claim 28 , ces0061 claim 28 , ces0067 claim 28 , ces0075 claim 28 , ces0076 claim 28 , ces0078 claim 28 , ces0089 claim 28 , ces0093 claim 28 , ces0094 claim 28 , and ces0095 claim 28 , wherein the at least one additional SSR allele has the number of base pairs (bp) as shown in Table 2 claim 28 , ±0.8 bp.30Epichloë. The combination of claim 28 , wherein the endophyte comprises at least two additional SSR alleles claim 28 , or at least 3 claim 28 , at least 4 claim 28 , at least 5 claim 28 , at least 6 claim 28 , at least 7 claim 28 , at least 8 claim 28 , at least 9 claim 28 , at least 10 claim 28 , at least 11 claim 28 , at least 12 claim 28 , at least 13 claim 28 , at least 14 claim 28 , at least 15 claim 28 , at least 16 claim 28 , at least 17 claim 28 , at least 18 claim 28 , at least 19 additional SSR alleles claim 28 , or 20 additional SSR alleles claim 28 , wherein each of the additional SSR alleles has the number of base pairs (bp) as ...

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Номер записи: 28

18-03-2021 дата публикации

Seed Endophytes Across Cultivars and Species, Associated Compositions, and Methods of Use Thereof

Номер: US20210076685A1

Автор: AMBROSE Karen V., BILLINGS Nathan A., Djonovic Slavica, Flavell Richard Bailey, GULICK Trudi A., Johnston David Morris, LEFF Jonathan W., LIVA Stephanie M., Lyford Jeffrey, Marquez Luis Miguel, Millet Yves Alain, Sadowski Craig, SAMAYOA Phillip, Toledo Gerardo V., von Maltzahn Geoffrey, WEISMAN David R., Zhang Xuecheng

Принадлежит:

Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes. 1. (canceled)2. (canceled)3. A method for preparing a seed comprising an endophyte population , said method comprising applying to an exterior surface of a seed a formulation comprising an endophyte population consisting essentially of an endophyte comprising a 16S rRNA or ITS rRNA nucleic acid sequence at least 95% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 1-455.47.-. (canceled)8. The method of claim 3 , wherein the endophyte is capable of a function or activity selected from the group consisting of auxin production claim 3 , nitrogen fixation claim 3 , production of an antimicrobial compound claim 3 , mineral phosphate solubilization claim 3 , siderophore production claim 3 , cellulase production claim 3 , chitinase production claim 3 , xylanase production claim 3 , and acetoin production.9. The method of claim 8 , wherein the endophyte exhibits at least two of: auxin production claim 8 , nitrogen fixation claim 8 , production of an antimicrobial compound claim 8 , mineral phosphate solubilization claim 8 , siderophore production claim 8 , cellulase production claim 8 , chitinase production claim 8 , xylanase production claim 8 , and acetoin production.10. The method of claim 3 , wherein the endophyte is capable of metabolizing at least one substrate selected from the group consisting of: a-D-glucose claim 3 , arabinose claim 3 , arbutin claim 3 , b-methyl-D-galactoside claim 3 , b-methyl-D-glucoside claim 3 , D-alanine claim 3 , D-arabitol claim 3 , D-aspartic acid claim 3 , D-cellobiose claim 3 , dextrin claim 3 , D-fructose claim 3 , D-galactose claim 3 , D-gluconic acid claim 3 , D-glucosamine claim 3 , dihydroxyacetone claim 3 , DL-malic acid claim 3 , D-mannitol claim 3 , D-mannose claim 3 , D-melezitose claim 3 , D- ...

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Номер записи: 29

05-03-2020 дата публикации

System and methods for the biocontrol of plant pathogens

Номер: US20200071713A1

Автор: Karen YIN, Pedro COSTA NUNES, Richard Sayre

Принадлежит: Pebble Labs Usa Inc

The inventive technology relates to novel systems, methods, and strategies for the control of plant pathogens and herbivores. The inventive technology may specifically include novel compositions, systems, and methods configured to deliver inhibitory RNA molecules to a pathogen-infected plant. In a preferred embodiment, the invention may include genetically engineered endophytic bacteria configured to deliver one or more inhibitory RNA molecules to a pathogen-infected plant. One preferred embodiment may include a novel trans-kingdom delivery of hairpin RNA targeting viral encoded proteins resulting in the reduction of viral protein accumulation levels.

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Номер записи: 30

21-03-2019 дата публикации

Endophytic Microbial Symbionts in Plant Prenatal Care

Номер: US20190082649A1

Автор: Germida James J., Vujanovic Vladimir

Принадлежит:

The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom. 120.-. (canceled)21Streptomyces. A method of increasing resistance to nitrogen stress in at least one agricultural crop , said method comprising mechanically or manually inoculating a seed of the agricultural crop with a composition comprising at least one endophyte and a carrier , wherein the at least one endophyte is of genus and comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6 or is deposited as IDAC 081111-06 and is capable of capable of causing populations of seeds to have increased yield under nitrogen stress conditions compared to control seeds.22. The method of claim 21 , wherein the endophyte is deposited as IDAC 081111-06.23. The method of claim 21 , wherein the endophyte comprises a 16S rDNA sequence with 100% sequence identity to SEQ ID NO:6.24. The method of claim 21 , wherein the agricultural crop is selected from the group consisting of cereal claim 21 , pulse claim 21 , and canola.25. The method of claim 21 , wherein the crop is a cereal.26. The method of claim 21 , wherein the crop is wheat.27. The method of claim 21 , wherein the seed is a durum wheat cultivar AC Avonlea seed.28. The method of claim 21 , wherein the nitrogen stress conditions result from lack of application of nitrogen to a field.29. The method of claim 21 , wherein the nitrogen stress conditions result from field nitrogen not present at agriculturally relevant rates.30. The method of claim 21 , wherein manually or mechanically inoculating the seed comprises coating the seed with the endophyte.31. The ...

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Номер записи: 31

25-03-2021 дата публикации

LEAF MARKERS FOR ROOT COLONIZATION BY ARBUSCULAR MYCORRHIZAL FUNGI IN PLANTS

Номер: US20210088516A1

Автор: BALDWIN Ian T., HALITSCHKE Rayko, HEILING Sven, LI DAPENG, MCGALE Erica, Schäfer Martin, Wang Ming

Принадлежит: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.

The invention relates to a method of determining an association of a first plant with an arbuscular mycorrhizal fungus (AMF), said method comprising comparing the amount of a blumenol in an aerial part of said first plant to the amount of said blumenol in an aerial part of a second plant, wherein said second plant belongs to the same species as said first plant, and wherein an increased amount is indicative of increased association in said first plant as compared to said second plant, and a decreased amount is indicative of decreased association. 1. A method of determining an association of a first plant with an arbuscular mycorrhizal fungus (AMF) , said method comprising comparing the amount of a blumenol in an aerial part of said first plant to the amount of said blumenol in an aerial part of a second plant , wherein said second plant belongs to the same species as said first plant , and wherein an increased amount is indicative of increased association in said first plant as compared to said second plant , and a decreased amount is indicative of decreased association.3. The method of any one of the preceding claims , wherein(i) said second plant is free of any association with an AMF;(ii) said second plant has an association with an AMF;(iii) said first and said second plant are the same individual at different points in time;(iv) said method comprises determining the amount of said blumenol in said first plant;(v) said method comprises determining the amount of said blumenol in said second plant; and/or(vi) said comparing is effected in a computer-implemented manner.4. Use of a blumenol as a marker for association of a plant with an AMF.5. A method of determining whether a plant has AMF receptivity , improved resource acquisition , or improved stress resistance;or of quantifying said receptivity, said improved acquisition, or said improved resistance;said method comprising:(a) contacting said plant with an AMF known to be capable of colonization; and(b) ...

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Номер записи: 32

01-04-2021 дата публикации

Selection of Symbiota by Screening Multiple Host-Symbiont Associations

Номер: US20210092933A1

Автор: Cocks Ben, Cogan Noel, Daetwyler Hans Dieter, Forster John White, Guthridge Kathryn Michaela, Hayden Matthew James, Hayes Ben, Mason John Gregory, Ran Yidong, Rochfort Simone Jane, Sawbridge Timothy Ivor, Spangenberg German Carlos

Принадлежит: AGRICULTURE VICTORIA SERVICES PTY LTD

The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organsims and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts seleced from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont. 1. A method of genomic selection of organism-symbiont associations , said method including:(i) providing a library of nucleic acid samples from said organism or said organism-symbiont association;(ii) analysing said samples using metagenomics to obtain a genetic profile for each sample;(iii) selecting organism-symbiont associations with a desired genetic and metabolic profile.2. The method according to including the preliminary steps of:(i) providing an organism-symbiont association;(ii) subjecting said organism-symbiont association to one or more environmental conditions; and(iii) preparing a library of nucleic acid samples from each of the environmentally treated organism-symbiont associations.3. The method according to claim 1 , wherein said organism is a plant.4. The method according to claim 3 , wherein the plant is a grass.5. The method according to claim 3 , wherein the symbiont is selected from the group consisting of a fungal or bacterial endophyte claim 3 , a fungal or bacterial epiphyte or a bacterial microbiome.6. The method according to claim 5 , wherein the symbiont is a bacterial microbiome.7. The method according to claim 1 , wherein the ...

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Номер записи: 33

12-05-2022 дата публикации

PEST CONTROL MATERIAL USING ENTOMOPARASITIC MICROBE AND PEST CONTROL METHOD USING SAME

Номер: US20220142174A1

Автор: INAI Koji, Kondo Akihiro

Принадлежит: IDEMITSU KOSAN CO.,LTD.

Aiming at improving long-term storage stability and the insect pest control effect of an insect pest control material utilizing a filamentous fungus, the present invention provides an insect pest control material including an entomogenous filamentous fungus grown on a solid support, wherein the spore fungal count of the entomogenous filamentous fungus in a surface layer is 1.0×10to 1.0×10cells/cm. 1. A method of producing an insect pest control material , the method comprising growing an entomogenous filamentous fungus on a solid support , wherein the spore fungal count of the entomogenous filamentous fungus in a surface layer of the solid support is 1.0×10to 1.0×10cells/cm.2BeauveriaPaecilomycesTrichodermaVerticilliumLecanicillium.. The method of claim 1 , wherein the entomogenous filamentous fungus is a fungus belonging to the genus claim 1 , a fungus belonging to the genus claim 1 , a fungus belonging to the genus claim 1 , a fungus belonging to the genus claim 1 , or a fungus belonging to the genus3Beauveria bassianaBeauveria brongniartii.. The method of claim 1 , wherein the entomogenous filamentous fungus is and/or4Beauveria bassianaBeauveria brongniartii. The method of claim 1 , wherein the entomogenous filamentous fungus is the F-263 (NITE BP-02855) strain and/or the NBL-851 (NITE BP-02854) strain.5. The method of claim 1 , wherein the insect pest is a plant insect pest or a hygiene insect pest.6. The method of claim 5 , wherein the plant insect pest is an insect pest of a tree.7. The method of claim 6 , wherein the insect pest of a tree is an insect pest belonging to the family Cerambycidae of the order Coleoptera.8. The method of claim 1 , wherein the solid support is an organic support.9. The method of claim 8 , wherein the organic support is husk claim 8 , sawdust claim 8 , bran claim 8 , wheat claim 8 , rice straw claim 8 , soybean claim 8 , soybean meal claim 8 , or plant residue.10. The method of claim 1 , wherein the solid support is an inorganic ...

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Номер записи: 34

12-05-2022 дата публикации

Corn Rootworm Biological Control

Номер: US20220142175A1

Автор: Cadle-Davidson Molly

Принадлежит: Agrauxine Corp.

Disclosed is a method for controlling corn rootworm damage to corn comprising the step of applying to a corn seed from 10to 10colony forming units of one of strain ARSEF 2579 (BF503), strain ARSEF 5197 (BF517) or mixtures thereof. Another aspect of the present disclosure comprises further combining either BF503 or BF517 with the fungi strain K2 (ATCC PTA-9708) plus strain K4 (ATCC PTA 9707) and applying it to corn seeds. Another aspect of the present disclosure comprises applying at least one of the BF503 or BF517 to a field planted with corn, with the fungi applied into the seed zone. In another aspect of the present disclosure the same field is further treated with both strain K2 (ATCC PTA-9708) plus strain K4 (ATCC PTA 9707) into the seed zone. 1. A method for reducing corn rootworm damage to corn plants comprising the step of:{'i': Beauveria bassiana', ', Metarhizium pingshaence, 'sup': 3', '6, 'applying to a plurality of corn seeds at least one fungus selected from the group consisting of strain ARSEF 2579strain ARSEF 5197 and mixtures thereof, each selected fungus applied at a level of from 10to 10colony forming units per seed.'}2. The method according to comprising the further step of planting the plurality of corn seeds.3Trichoderma afroharzianumTrichoderma atroviride. The method according to comprising the further step of applying to the plurality of corn seeds from 10to 10colony forming units of species K2 and 10to 10colony forming units of species K4.4. The method according to comprising the further step of planting the plurality of corn seeds.5. A method for reducing corn rootworm damage to corn comprising the step of:{'i': Beauveria bassiana', ', Metarhizium pingshaence, 'sup': 3', '6, 'applying to a field planted with corn seeds at least one fungus selected from the group consisting of strain ARSEF 2579strain ARSEF 5197 and mixtures thereof, the selected fungus applied into the seed zone of each seed at a level of from 10to 10colony forming units per ...

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Номер записи: 35

29-04-2021 дата публикации

INSECT PATHOGENIC MICROORGANISM-CONTAINING AGRICULTURAL PESTICIDE FORMULATION AND MANUFACTURING METHOD THEREFOR

Номер: US20210120810A1

Автор: KOO Kyung Bon, LEE Hyung Chul

Принадлежит:

The present disclosure relates to a pesticide formulation containing an insect-pathogenic microorganism and a method of manufacturing the same, and more particularly to a granular or powder pesticide formulation containing an insect-pathogenic microorganism and a method of manufacturing the same. The granular pesticide formulation of the present disclosure includes (a) a porous carrier, (b) a medium infiltrated into pores in the porous carrier, and (c) an insect-pathogenic microorganism cultured in the medium. The pesticide formulation containing the insect-pathogenic microorganism according to the present disclosure has a superior insecticidal effect because the number of spores of the insect-pathogenic microorganism having insecticidal activity is significantly increased compared to existing formulations. 1. A granular pesticide formulation , comprising:(a) a porous carrier;(b) a medium infiltrated into pores in the porous carrier; and(c) an insect-pathogenic microorganism cultured in the medium.2. The granular pesticide formulation of claim 1 , wherein the porous carrier is a porous ceramic ball or sponge.3. The granular pesticide formulation of claim 2 , wherein the porous ceramic ball is selected from the group consisting of a zeolite ball claim 2 , a terra alba ball claim 2 , a loess ball claim 2 , a magnetite ball claim 2 , and a diatomite ball.4. The granular pesticide formulation of claim 2 , wherein the sponge is foam rubber or a urethane-based resin.5. The granular pesticide formulation of claim 1 , wherein the medium is selected from the group consisting of whole milk powder claim 1 , skim milk powder claim 1 , whey claim 1 , milk claim 1 , wheat flour claim 1 , starch claim 1 , and grain powder.6. The granular pesticide formulation of claim 1 , wherein the insect-pathogenic microorganism is a fungus that has insecticidal activity against pests and forms spores.7. A method of manufacturing a granular pesticide formulation claim 1 , comprising:(a) ...

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Номер записи: 36

29-04-2021 дата публикации

Methods of Screening for Microorganisms that Impart Beneficial Properties to Plants

Номер: US20210123043A1

Автор: GEORGE Caroline Elizabeth, Turner Susan Jane, Wigley Peter John

Принадлежит:

The present invention relates to methods for the screening, identification and/or application of microorganisms and/or compositions of use in imparting beneficial properties to plants, and microorganisms and compositions identified therefrom. 1. A method of imparting a beneficial trait upon a plant , comprising:{'i': Arthrobacter cupressi', 'Kosakonia radicincitans., 'applying at least one bacterial species to a plant or to a growth medium having a plant, wherein the at least one bacterial species is selected from and'}2. The method of claim 1 , wherein the applying occurs by:coating a plant seed with the bacteria thereby resulting in a bacterial seed coating,coating a plant part with the bacteria,spraying the bacteria onto a plant part,spraying the bacteria into a furrow into which a plant or seed will be placed,drenching the bacteria onto a plant part or into an area into which a plant will be placed,spreading the bacteria onto a plant part or into an area into which a plant will be placed,broadcasting the bacteria onto a plant part or into an area into which a plant will be placed,combining the bacteria with a fertilizer or other agricultural composition, and combinations thereof.3. The method of claim 1 , wherein the isolated bacterial strain is formulated in an agricultural composition with one or more of the following: an agriculturally acceptable carrier claim 1 , a pesticide claim 1 , a plant growth regulator claim 1 , a beneficial agent or a biologically active agent.4Bradyrhizobium betae, Bradyrhizobium elkanii, Bradyrhizobium japonicum, Enterobacter soli, Klebsiella variicola, Kosakonia oryzae, Paenibacillus azotifigens, Paenibacillus borealis, Paenibacillus graminis, Paenibacillus odorifer, Paenibacillus polymyxa, Paenibacillus taohuashanense, Rahnella aquatilisBacillus amyloliquefaciens.. The method of claim 1 , further comprising applying at least one additional bacterial species from the group consisting of: claim 1 , and5Arthrobacter cupressi. An ...

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Номер записи: 37

13-05-2021 дата публикации

ENDOPHYTE COMPOSITIONS AND METHODS FOR IMPROVEMENT OF PLANT TRAITS

Номер: US20210139928A1

Автор: Riley Raymond

Принадлежит:

This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes. 1133.-. (canceled)134Acremonium. A synthetic composition comprising a soybean plant seed and a heterologously disposed endophyte , wherein the endophyte is a member of the genus that is heterologous to the soybean plant seed and comprises SEQ ID NO: 51 , wherein the endophyte is heterologously disposed to the soybean plant seed in an amount effective to colonize a soybean plant germinated from the synthetic composition and to improve a trait of agronomic importance compared to a control soybean plant germinated from a control soybean plant seed lacking the heterologously disposed endophyte.135147.-. (canceled)148. The synthetic composition of claim 134 , wherein the improved trait of agronomic importance is conferred under normal watering conditions.149153.-. (canceled)154. The synthetic composition of further comprising a formulation that comprises one or more of the following: stabilizer claim 134 , preservative claim 134 , carrier claim 134 , surfactant claim 134 , anticomplex agent claim 134 , or any combination thereof.155. The synthetic composition of further comprising a formulation that comprises one or more of the following:fungicide, nematicide, bactericide, insecticide, or herbicide.156. (canceled)157. The synthetic composition of claim 134 , wherein the synthetic combinations are shelf-stable.158165.-. (canceled)166. The synthetic combination of claim 134 , wherein the trait of agronomic importance is selected from the group consisting of an increase in dry shoot biomass claim 134 , an increase in root area claim 134 , an increase in yield claim 134 , an increase in root length claim 134 , and an increase in average root length by at least 10% as measured ...

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Номер записи: 38

05-05-2016 дата публикации

ACCELERATED DIRECTED EVOLUTION OF MICROBIAL CONSORTIA FOR THE DEVELOPMENT OF DESIRABLE PLANT PHENOTYPIC TRAITS

Номер: US20160122750A1

Автор: GEORGE Caroline, TURNER Susan, Wigley Peter

Принадлежит: Biodiscovery New Zealand Limited

The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants. 1. A plant-mediated method for directed evolution of a plant microbiome , to create a microbial community associated with imparting a beneficial phenotypic trait to a plant , comprising:a) growing a plurality of plants in a growth medium, in the presence of a first microbial community;b) selecting at least one plant from said plurality, following step a), based upon a beneficial phenotypic trait exhibited by the plant relative to other plants of said plurality;c) acquiring a second microbial community from the at least one plant selected in step b);d) repeating steps a) to c) in an iterative manner, wherein the second microbial community acquired in step c) is used as an inoculum in step a) of any successive repeat; ande) acquiring a microbial community that is associated with imparting a beneficial phenotypic trait to a plant.2. The method according to claim 1 , further comprising: isolating at least one microbe from the microbial community acquired in step e).3. The method according to claim 1 , further comprising: isolating at least one microbe from the microbial community acquired in step e) and utilizing a molecular technique to characterize the isolated microbe.4. The method according to claim 1 , wherein the plurality of plants are members of the Poaceae family.5Zea, Sorghum, Panicum, Oryza, Triticum, Hordeum, Secale, TriticaleAvena.. The method according to claim 1 , wherein the plurality of plants are members of a genus selected from the group consisting of: claim 1 , or6. The method according to claim 1 , wherein the plurality of plants are legumes.7. The method according to claim 1 , wherein the plurality of plants are cotton plants.8. The method according to claim 1 , wherein the plurality of plants are soybean plants.9Zea mays. The method according to claim 1 , ...

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Номер записи: 39

01-09-2022 дата публикации

Novel Xanthomonas Strains and Related Methods

Номер: US20220272935A1

Автор: Kaur Jatinder, Krill Christian, Li Tongda, Mann Ross, Sawbridge Timothy Ivor, Spangenberg German Carlos, Tannenbaum Ian Ross

Принадлежит:

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of sp. which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods. 123-. (canceled)24Xanthomonas. A substantially purified or isolated endophyte strain isolated from a plant of the Poaceae family , wherein said endophyte is a strain of sp. which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated.25. The endophyte according to claim 24 , wherein the bioprotection and/or biofertilizer phenotype includes production of the bioprotectant compound in the plant into which the endophyte is inoculated.26. The endophyte according to claim 25 , wherein the bioprotectant compound is selected from the group consisting of siderophore xanthoferrin claim 25 , and/or xanthomonadin claim 25 , or a derivative claim 25 , isomer and/or salt thereof.27. The endophyte according to claim 24 , wherein the bioprotection and/or biofertilizer phenotype is selected from the group consisting of production of organic acids claim 24 , solubilisation of phosphate and nitrogen fixation in the plant into which the endophyte is inoculated.28Xanthomonas. The endophyte according to claim 24 , wherein the endophyte is strain is selected from the group consisting of sp. strains GW claim 24 , SS and SI as deposited with The National Measurement Institute on 17 May 2019 with accession numbers V19/009902 claim 24 , V19/009905 and V19/009909 claim 24 , respectively.29. The endophyte according to claim 24 , wherein the plant from which the endophyte is isolated is a pasture grass.30LoliumFestuca.. The endophyte according to claim 29 , wherein the pasture grass is from the genus or31Lolium perenneFestuca arundinaceum.. The endophyte according to claim 30 , wherein the pasture grass is from the species or32. ...

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Номер записи: 40

01-09-2022 дата публикации

Novel Stenotrophomonas Strains and Related Methods

Номер: US20220272985A1

Автор: Kaur Jatinder, Krill Christian, Li Tongda, Mann Ross, Sawbridge Timothy Ivor, Spangenberg German Carlos, Tannenbaum Ian Ross

Принадлежит:

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods. 134-. (canceled)35Stenotrophomonas rhizophila. A substantially purified or isolated endophyte strain isolated from a plant of the Poaceae family , wherein said endophyte is a strain of which provides bioprotection and/or biofertilizer phenotypes to plants into which it is inoculated.36. The endophyte according to claim 35 , wherein the bioprotection and/or biofertilizer phenotype includes production of a bioprotectant compound in the plant into which the endophyte is inoculated.37. The endophyte according to claim 36 , wherein the bioprotectant compound is spermidine claim 36 , or a derivative claim 36 , isomer and/or salt thereof.38. The endophyte according to claim 35 , wherein the bioprotection and/or biofertilizer phenotype is selected from the group consisting of production of organic acids claim 35 , solubilization of phosphate and nitrogen fixation in the plant into which the endophyte is inoculated.39Stenotrophomonas rhizophila. The endophyte according to claim 35 , wherein the endophyte is strain JB as deposited with The National Measurement Institute on 17May 2019 with accession number V19/009906.40. The endophyte according to claim 35 , wherein the plant from which the endophyte is isolated is a pasture grass.41LoliumFestuca,Lolium perenne. The endophyte according to claim 40 , wherein the pasture grass is from the genus or preferably from the species or Festuca arundinaceum.42. The endophyte according to claim 35 , wherein the plant into which the endophyte is inoculated includes an endophyte-free host plant or part thereof stably infected with said endophyte.43. The endophyte according to claim 35 , wherein the plant into which ...

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Номер записи: 41

17-05-2018 дата публикации

METHOD FOR PRODUCING PLANT SEED CONTAINING ENDOPHYTIC MICRO-ORGANISMS

Номер: US20180132486A1

Автор: MITTER Birgit, Naveed Muhammad, SESSITSCH Angela

Принадлежит:

The invention discloses a method for producing plant seed containing endophytic microorganisms characterised in by the following steps: 1. A method for producing a bacterial colonized plant seed comprising inoculant endophytic microorganisms , the method comprising:contacting at least one flower of a flowering plant in the course of a flowering phase of the flowering plant with a preparation comprising a population of inoculant endophytic microorganisms, wherein the preparation comprises 10̂6 to 10̂10 cfu per mL, whereby the inoculant endophytic microorganisms enter the flowering plant via the at least one flower and are conveyed to an interior of at least one seed produced by the flowering plant; andobtaining from the contacted flowering plant the bacterial colonized plant seed comprising inoculant endophytic microorganisms.2Burkholderia, Rhizobium, Bradyrhizobium, MesorhizobiumSinorhizobium, Herbaspirillum, Azospirillum, Acetobacter, Arthrobacter, Bacillus, Paenibacillus, Streptomyces, Pantoea, EnterobacterPseudomonas.. The method of claim 1 , wherein the inoculant endophytic microorganism is an endophytic bacterium selected from claim 1 , and claim 1 , and3Burkholderia phytofirmans.. The method of claim 1 , wherein the inoculant endophytic microorganism is4. The method of claim 1 , wherein contacting the at least one flower with the preparation is performed via spraying the preparation on the flower at the time of flowering.5. The method of claim 1 , wherein the preparation comprises 10to 10cfu/mL of inoculant endophytic microorganisms.6. The method of claim 1 , wherein the bacterial colonized plant seed containing inoculant endophytic microorganisms is stored for at least 1 month claim 1 , for at least 3 months claim 1 , for at least 6 months claim 1 , for at least 12 months claim 1 , for at least 2 years claim 1 , or for at least 3 years.7. (canceled)8. (canceled)9. The method of claim 1 , wherein the inoculant endophytic microorganism is a recombinantly ...

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Номер записи: 42

15-09-2022 дата публикации

Methods and compositions for the production of ectomycorrhizal mycelia and methods of use thereof

Номер: US20220287251A1

Автор: Brian L. Upchurch, Davis M. Upchurch

Принадлежит: Carolina Truffieres LLC

The invention relates to methods of cultivating ectomycorrhizal fungi, the method comprising incubating ectomy corrhizal MAT1-1 mycelia, ectomycorrhizal MAT1-2 mycelia, and ectomycorrhizal male mycelia in a culture medium for about 1 day to about 50 days, thereby cultivating ectomycorrhizal fungi to produce an ectomycorrhizal fungal biomass comprising MAT1-1 ectomycorrhizal mycelia, MAT1-2 ectomycorrhizal mycelia, and male ectomycorrhizal mycelia. Also provided are methods of use of the ectomycorrhizal mycelial biomass.

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Номер записи: 43

07-05-2020 дата публикации

Non-Toxic Plant Agent Compositions and Methods and Uses Thereof

Номер: US20200138037A1

Автор: Dale Parker, Dale Parker David

Принадлежит: Neozyme International, Inc.

The present specification discloses plant agent compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to control a causal agent of a plant disease or increasing plant growth and/or fruit production. 1. A dry powdered composition comprising at most 20.0% by weight of a dried treated , fermented yeast supernatant including bio-nutrients , minerals and amino acids , and one or more nonionic biosurfactants , wherein the composition lacks active enzymes contributed by yeast during fermentation due to the treatment , and wherein the composition has a pH of about 2.5 to about 5.0.2Brettanomyces, Candida, Cyberlindnera, Cystofilobasidium, Debaryomyces, Dekkera, Fusarium, Geotrichum, Issatchenkia, Kazachstania, Kloeckera, Kluyveromyces, Lecanicillium, Mucor, Neurospora, Penicillium, Pichia, Rhizopus, Rhodosporidium, Rhodotorula, Saccharomyces, Schizosaccharomyces, Thrichosporon, Torulaspora, Torulopsis, Verticillium, Yarrowia, ZygosaccharomycesZygotorulaspora.. The dry powdered composition according to claim 1 , wherein the dried treated fermented yeast supernatant is produced from a culture containing yeast belonging to the genus claim 1 , or3. The dry powdered composition according to claim 1 , comprising at most 15% by weight of the dried treated claim 1 , fermented yeast supernatant.4. The dry powdered composition according to claim 3 , comprising at most 10% by weight of the dried treated claim 3 , fermented yeast supernatant.5. The dry powdered composition according to claim 1 , comprising about 5% to about 15% by weight of the dried treated claim 1 , fermented yeast supernatant.6. The dry powdered composition according to claim 5 , comprising about 5% to about 10% by weight of the dried treated claim 5 , fermented yeast supernatant.7. The dry powdered composition according to claim 1 , comprising at least 10.0% by weight of the one or more nonionic biosurfactants.8. The dry powdered composition according to claim ...

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Номер записи: 44

17-06-2021 дата публикации

FUNGICIDALLY ACTIVE COMBINATION OF A CLONOSTACHYS STRAIN AND AN AZOLE

Номер: US20210176992A1

Автор: Dahmen Peter

Принадлежит:

The present invention relates to a composition comprising a fungicidally active strain and a fungicidally active azole and methods of using this composition for reducing the DON content in cereal grains infected with head blight. 1C. rosea f. catenulata. A composition comprising a fungicidally active strain and a fungicidally active azole.2. The composition according to claim 1 , wherein the azole is prothioconazole or epoxiconazole.3C. rosea f. catenulataFusarium. The composition according to claim 1 , wherein said fungicidally active strain is active against head blight.4C. rosea f. catenulataC. rosea f. catenulatum. The composition according to claim 1 , wherein said strain is J1446 deposited under accession number DSM 9212.5C. rosea f. catenulata. The composition according to claim 1 , wherein said strain is present in the form of spores claim 1 , mycelia or conidia.6C. rosea f. catenulata. The composition according to claim 1 , wherein the ratio between said strain and said fungicidally active azole is between about 20:1 and about 1:20.7. The composition according to claim 1 , further comprising at least one additional fungicide provided that said additional fungicide is not identical to said fungicidally active azole.8. The composition according to claim 7 , wherein said at least one additional fungicide is selected from the group consisting of tebuconazole claim 7 , pydiflumetofen claim 7 , metconazole claim 7 , prochloraz and thiophanate-methyl.9. The composition according to additionally comprising at least one auxiliary selected from the group consisting of extenders claim 1 , solvents claim 1 , spontaneity promoters claim 1 , carriers claim 1 , emulsifiers claim 1 , dispersants claim 1 , frost protectants claim 1 , thickeners and adjuvants.10. A seed coated with the composition according to .11Fusarium. A method of reducing the deoxynivalenol content in cereal grains infected with head blight claim 1 , comprising simultaneously or sequentially applying an ...

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Номер записи: 45

11-06-2015 дата публикации

Selection of Symbiota by Screening Multiple Host-Symbiont Associations

Номер: US20150156982A1

Автор: Cocks Ben, Cogan Noel, Daetwyler Hans Dieter, Forster John White, Guthridge Kathryn Michaela, Hayden Matthew James, Hayes Ben, Mason John Gregory, Ran Yidong, Rochfort Simone Jane, Sawbridge Timothy Ivor, Spangenberg German Carlos

Принадлежит: AGRICULTURE VICTORIA SERVICES PTY LTD

The present invention relates to new methods of selecting and breeding organisms, in particular organisms which exhibit symbiotic behaviour with symbionts, and to new organisms and symbiota developed thereby, such as plant or grass and endophyte symbiota. Multiple symbionts are deployed in multiple organisms and selected for improved symbiotic compatibility and performance early in the breeding process. Methods include producing improved organisms from germplasm, by inoculating host organism germplasm libraries with symbionts selected from symbiont libraries and selecting improved host organisms exhibiting desired symbiota characteristics, and selection of organism-symbiont associations with a desired genetic and metabolic profile by metagenomic analysis of nucleic acid libraries from an organism or organism-symbiont association. 1. A method for producing an improved organism including: 'a library of symbionts;', '(i) providing a library of germplasm of an organism; and'}(ii) inoculating the germplasm library with one or more symbionts selected from the symbiont library to generate symbiota;(iii) breeding, selecting, screening and/or evaluating the inoculated germplasm or symbiota for desired symbiota characteristic(s); and(iv) subsequently identifying, culturing or otherwise using the symbiota exhibiting desired characteristic(s) to produce the improved organism.2. The method according to claim 1 , wherein the organism is a plant or animal.3. The method according to claim 2 , wherein the symbiont(s) is capable of forming symbiotic associations with the plant or animal.4. The method according to claim 3 , wherein the symbiont is selected from one or more of fungi claim 3 , viruses claim 3 , bacteria and other microbes.5. The method according to claim 4 , wherein the symbiont(s) includes a genetic variation selected to enhance symbiont trait introgression.6. The method according to claim 5 , wherein the genetic variation is introduced via one or more of random ...

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Номер записи: 46

18-06-2015 дата публикации

EFFECTIVE HEAT TREATMENT FOR THE DEVITALIZATION OF REFERENCE SEED MATERIAL

Номер: US20150164012A1

Автор: Cruse James K., Embrey Shawna K.

Принадлежит:

A method for devitalizing a seed includes heating the seed to a temperature from about 95° C. to about 100° C. for a predetermined period, wherein said heating step completely devitalizes the seed. 1. A method for devitalizing a seed comprising:heating the seed to a temperature from about 95° C. to about 100° C. for a period of about 4 hours to about 14 days, wherein said heating step completely devitalizes the seed.2. The method of claim 1 , wherein the temperature is about 95° C.3. The method of claim 1 , wherein the temperature is about 100° C.4. The method of claim 1 , wherein the seed following the heating step has a concentration of a typical protein readily detectable by an ELISA quantification method.5. The method of claim 4 , wherein the typical protein has a molecular weight of about 20 claim 4 ,000 Dalton to about 130 claim 4 ,000 Dalton.6. The method of claim 4 , wherein the typical protein has a molecular weight of about 14 claim 4 ,000 Dalton to about 250 claim 4 ,000 Dalton.7. The method of claim 4 , wherein the typical protein is a protein is selected from the group consisting of: AAD-12 claim 4 , Cry1F claim 4 , Cry1Ac claim 4 , PAT claim 4 , Cry34Ab1 claim 4 , Cry35Ab1 claim 4 , and AAD-1.8. The method of claim 1 , wherein the seed exhibits minimal or no genomic DNA degradation following said heating step.9. The method of claim 1 , wherein the seed has no genomic DNA degradation following said heating step compared to a similar unheated seed.10. The method of claim 1 , wherein the seed is transgenic.11. The method of claim 1 , wherein the seed is maize.12. The method of claim 11 , wherein the predetermined period is from about 4 hours to about 24 hours.13. The method of claim 1 , wherein the seed is cotton.14. The method of claim 13 , wherein the predetermined period is about 1 day to about 9 days.15. The method of claim 1 , wherein the seed is soybean.16. The method of claim 15 , wherein the predetermined period is about 1 day to about 6 days.17. ...

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Номер записи: 47

29-09-2022 дата публикации

COMPOSITIONS AND METHODS FOR MODIFYING A PLANT CHARACTERISTIC WITHOUT MODIFYING THE PLANT GENOME

Номер: US20220304273A1

Автор: Grando Magali Ferrari, Heck Michelle L., Krystel Joseph, Niedz Randall P., Pitino Marco, Shatters Robert G., STOVER Eddie W.

Принадлежит:

The invention relates to methods and compositions for modifying a characteristic of a plant without modifying the plant's genome using one or more cells comprising one or more phytohormone genes and at least one polynucleotide of interest, which one or more phytohormone genes and the at least one polynucleotide of interest are expressed in the one or more cells. 1. A symbiont forming inoculum comprising a polynucleotide encoding a phytohormone biosynthetic enzyme and a polynucleotide of interest , wherein the phytohormone biosynthetic enzyme is at least one cytokinin biosynthetic enzyme and/or an auxin biosynthetic enzyme.2. The symbiont forming inoculum of claim 1 , wherein the polynucleotide encoding a phytohormone biosynthetic enzyme and the polynucleotide of interest are comprised in a cell claim 1 , optionally wherein the cell is a plant cell or a bacterial cell.3. The symbiont forming inoculum of or claim 1 , wherein the phytohormone biosynthetic enzyme is from a bacterial species and/or a plant species.4. The symbiont forming inoculum of any one of - claim 1 , wherein the phytohormone biosynthetic enzyme is an indole-3-acetamide hydrolase (iaaH) (E.C. Number: EC 3.5.1.4) claim 1 , amidase 1 (EC 3.5.1.4) claim 1 , a tryptophan 2-monooxygenase (IaaM) (EC 1.13.12.3) claim 1 , an indole-3-lactate synthase (EC 1.1.1.110) claim 1 , a L-tryptophan-pyruvate aminotransferase 1 (EC 2.6.1.99) claim 1 , a tryptophan aminotransferase-related protein 1 (EC 2.6.1.27) claim 1 , indole-3-acetaldehyde oxidase (EC 1.2.3.7) claim 1 , a tryptophan decarboxylase 1/tryptophan decarboxylase 2 (EC4.1.1.105) claim 1 , an isopentenyl transferase (Ipt) and/or a Tzs (EC 2.5.1.27).5. The symbiont forming inoculum of any one of - claim 1 , wherein the phytohormone biosynthetic enzyme is an indole-3-acetamide hydrolase (iaaH) claim 1 , a tryptophan 2-monooxygenase (IaaM) claim 1 , and/or an isopentenyl transferase (Ipt).6. The symbiont forming inoculum of any one of - claim 1 , wherein the ...

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Номер записи: 48

29-09-2022 дата публикации

MODULATED NUTRITIONAL QUALITY TRAITS IN SEEDS

Номер: US20220304314A1

Автор: Bitas Vasileios, Djonovic Slavica, Riley Raymond, Vosnidou Nancy

Принадлежит:

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte. 172-. (canceled)73EnterobacterEnterobacterEnterobacter. A method of increasing crude fat content of a seed produced by a wheat plant , comprising mechanically or manually inoculating a wheat plant seed with a treatment formulation comprising an agriculturally compatible carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus that is heterologous to the wheat plant seed and comprises a polynucleotide sequence having at least 97% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 23 or SEQ ID NO: 62 , wherein the endophyte is heterologously disposed to the wheat plant seed in an amount effective to colonize a wheat plant germinated from the inoculated wheat plant seed , wherein a seed obtained from the colonized wheat plant exhibits an increase in crude fat content as compared to a reference wheat plant seed obtained from a reference wheat plant lacking the colonized endophyte of the genus , and wherein said reference wheat plant is germinated from a reference wheat plant seed lacking the colonized endophyte of the genus comprising the polynucleotide sequence having at least 97% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 23 or SEQ ID NO: 62.74. The method of claim 73 , wherein the seed bacterial endophyte comprises a polynucleotide sequence having at least 97% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 23.75. The method of claim 73 , wherein the seed bacterial endophyte comprises a polynucleotide sequence having at least 97% nucleotide sequence identity to the polynucleotide sequence as set forth in SEQ ID NO: 62.76. The method of claim 73 , ...

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Номер записи: 49

15-06-2017 дата публикации

ENDOPHYTE ENHANCED SEEDLINGS WITH INCREASED PEST TOLERANCE

Номер: US20170164621A1

Автор: Adams Greg William, Miller John David

Принадлежит:

The invention provides methods for preparing a conifer seedling with increased tolerance to a pest. A conifer seedling is inoculated with an isolated endophyte when the conifer seedling is susceptible to colonization by the endophyte. 2. (canceled)3. The method of wherein the inoculum composition comprises the toxigenic endophyte claim 1 , as hyphal fragments and the inoculum composition comprises at least 1-25 toxigenic endophyte hyphal fragments/6 microliter claim 1 , optionally at least 1-4 toxigenic endophyte hyphal fragments/microliter claim 1 , optionally at least 0.2-4 toxigenic endophyte hyphal fragments/microliter claim 1 , or at least 3 toxigenic endophyte hyphal fragments per 6 microliter.412-. (canceled)13. The method of wherein the inoculating step comprises spraying the seedling with the inoculum.1422.-. (canceled)23. The method of wherein the conifer seed is contacted with the isolated toxigenic endophyte by soaking the seed in a solution comprising the toxigenic endophyte.24. The method of comprising repeating the inoculation step.2527.-. (canceled)28. The method of claim 1 , wherein the seedling or seed is grown in a greenhouse until the toxigenic endophyte is present in an amount adequate to reduce pest growth rate claim 1 , pest development and/or reduce pest weight gain compared to pest growth in a non-inoculated conifer seedling.29. (canceled)30. The method of claim 1 , wherein the toxigenic endophyte produces at least one toxin compound selected from the group consisting of rugulosin claim 1 , vermiculin claim 1 , 5-methoxy-carbonylmellein claim 1 , 5-formylmellein claim 1 , 5-methylmellein claim 1 , 3-butyl-4-methylfuran-2(5H)-one butyrolactone tyrosol claim 1 , mellein claim 1 , 8-hydroxy-6-methoxy-3-propyl-3 claim 1 ,4-dihydroisocoumarin claim 1 , and 3-methyl-5 claim 1 ,7-dimethoxyphthalide.31. (canceled)32. The method of wherein the toxin compound is rugulosin or vermiculin and the seedling or seed is grown until the toxin compound is ...

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Номер записи: 50

21-05-2020 дата публикации

FUNGAL IMMUNOGENS AND RELATED MATERIALS AND METHODS

Номер: US20200155657A1

Автор: Narra Hema P., Orbach Marc J., Shubitz Lisa F.

Принадлежит: Arizona Board of Regents on Behalf of the University of Arizona

The present disclosure provides immunogenic materials and methods useful for reducing the risk of fungal infections, particularly valley fever. The disclosure also provides assays for identifying compounds useful to treat valley fever, as well as methods for ameliorating the symptoms of valley fever. 143-. (canceled)44. An immunogenic vaccine composition , comprising:{'i': 'Coccidioides', 'An avirulent arthroconidia comprising a genetically modified or missing cyclic peptide synthase (CPS1) protein; and'}a pharmaceutically acceptable carrier.45. The immunogenic vaccine composition of claim 44 , wherein the composition is formulated for human administration.46. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for intranasal administration.47. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for subcutaneous administration.48. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for intramuscular administration.49. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of at least a portion of the CPS1 gene encoding the CPS1 protein.50. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of the DNA methyltransferase-associated protein (DMAP) binding domain region of the CPS1 gene encoding the CPS1 protein.51. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of the adenosine monophosphate (AMP) binding domain region of the CPS1 gene encoding the CPS1 protein.52. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing as a result of a deletion of at least one regulatory element of the CPS1 gene encoding the CPS1 protein.53. The immunogenic vaccine composition of claim 44 , ...

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Номер записи: 51

21-05-2020 дата публикации

DENDROBIUM OFFICINALE ENDOPHYTIC FUNGUS STRAIN AND EXTRACELLULAR POLYSACCHARIDE PRODUCED THEREBY, AND EXTRACTION METHOD AND APPLICATION OF EXTRACELLULAR POLYSACCHARIDE

Номер: US20200157590A1

Автор: Liang Jing, Lou Wenyong, Wang Hongfeng, Zeng Yingjie, ZONG Minhua

Принадлежит:

The present invention provides a () endophytic fungus strain and an extracellular polysaccharide produced thereby as well as an extraction method and application of the extracellular polysaccharide. The extraction process of the extracellular polysaccharide includes: activating a strain; culturing seed liquid; fermenting and culturing; filtering the fermented liquid under vacuum; concentrated the filtrate under vacuum; conducting alcohol precipitation on the concentrated liquid; precipitating and centrifuging; dialyzing after dissolving the precipitates in water; freezing and drying the dialysate under vacuum; and obtaining the extracellular polysaccharide. The endophytic fungus strain belongs to , and the extracellular polysaccharide produced thereby has inhibitory effects on ssp., and 1Dendrobium officinaleD. officinaleFusarium solaniFusarium solani. A () endophytic fungus strain , belonging to , named DO7 , and preserved at China Center for Type Culture Collection (CCTCC) on Mar. 27 , 2017 with the accession number of CCTCC NO. M2017145.2D. officinale. An extraction method of extracellular polysaccharide produced by the endophytic fungus strain according to claim 1 , comprising the following steps:{'i': 'D. officinale', '(1) activating the endophytic fungus strain, culturing seed liquid, and then fermenting and culturing the obtained seed liquid; and'}{'i': 'D. officinale', '(2) filtering the fermented liquid obtained after the fermenting and culturing in step (1) under vacuum; conducting vacuum concentration, alcohol precipitation and centrifugation on a filtrate obtained; dehydrating and drying centrifuged precipitates, dissolving in deionized water and dialyzing, and freezing and drying the dialysate under vacuum, to obtain the extracellular polysaccharide produced by the endophytic fungus strain.'}3. The extraction method according to claim 2 , wherein in step (1) claim 2 , the activating and culturing is to use a slant culture method for activation in a test ...

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Номер записи: 52

21-06-2018 дата публикации

GLOMUS IRANICUM VAR. TENUIHYPHARUM VAR. NOV. STRAIN AND USE THEREOF AS BIOSTIMULANT

Номер: US20180168167A1

Автор: FERNANDEZ Felix, JUAREZ Jesus

Принадлежит:

var. var. nov. strain deposited under BCCM deposit number 54871, comprising the sequence identified by SEQ ID NO: 1; composition having the strain and 2:1 smectite clays and use thereof as bio-stimulant. The invention also discloses a composition having the strain, fungicides, bio-fungicides, insecticides, bio-insecticides, nematicides and bio-nematicides. 1Glomus iranicumtenuihypharum. A method for biostimulating , regulating or enhancing plant growth comprising applying an effective amount of a composition comprising a strain of var. var. nov. deposited under BCCM deposit number 54871 comprising the sequence identified by SEQ ID NO: 1 and 2:1 smectite clays.2. The method according to claim 1 , wherein said 2:1 smectite clays are dioctahedral or trioctahedral.3. The method according to claim 1 , wherein said 2:1 smectite clays are selected from the group consisting of sepiolite claim 1 , attapulgite claim 1 , nontronite and saponite.4Glomus iranicumtenuihypharum. The method according to claim 1 , wherein the concentration of said var. var. nov. strain is between 0.05 and 4% by weight.5. The method according to claim 4 , wherein said concentration is between 0.1 and 3% by weight.6. The method according to claim 1 , wherein the composition is in a form of powder claim 1 , emulsifiable concentrate or granules.7. The method according to claim 1 , wherein the composition is a liquid claim 1 , a solid or a gel.8. The method according to claim 1 , wherein the composition further comprises at least one fungicide claim 1 , at least one bio-fungicide claim 1 , at least one insecticide claim 1 , at least one bio-insecticide claim 1 , at least one nematicide and/or at least one bio-nematicide.9. The method according to claim 8 , wherein said fungicide is selected from the group consisting of Maneb claim 8 , Mancozeb claim 8 , Metalaxyl-Ridomil claim 8 , Myclobutanil claim 8 , Olpisan claim 8 , Propamocarb claim 8 , Quintozene claim 8 , Streptomycin claim 8 , Sulfur claim 8 , ...

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Номер записи: 53

08-07-2021 дата публикации

Streptomyces Endophyte Compositions and Methods for Improved Agronomic Traits in Plants

Номер: US20210204505A1

Автор: AMBROSE Karen V., Djonovic Slavica, GULICK Trudi A., Johnston David Morris, MCKENZIE Elizabeth Alexa, Sadowski Craig, TOLEDO Gerardo, von Maltzahn Geoffrey, Zhang Xuecheng

Принадлежит:

This invention relates to methods and compositions for providing a benefit to a plant by associating the plant with a beneficial endophyte of the genus , including benefits to a plant derived from a seed or other plant element treated with said endophyte. For example, this invention provides purified endophytes, synthetic combinations comprising endophytes, and methods of making and using the same. In particular, this invention relates to compositions and methods of improving soybean and maize plants. 140.-. (canceled)41StreptomycesStreptomyces. A method of enhancing symbiosis in a plant grown from a plant reproductive element , comprising treating the plant reproductive element with a formulation comprising an effective amount of a endophyte that is heterologous to the plant reproductive element , wherein the endophyte comprises a 16S polynucleotide sequence having at least 97% identity to SEQ ID NO 2; and at least 3 polynucleotides that encode an arabinose transporter protein;{'i': 'Streptomyces', 'wherein the effective amount is effective to increase expression of one or more genes encoding a sugar transporter domain in a plant grown from the treated plant reproductive element, as compared to an isoline plant grown from a plant reproductive element not treated with the endophyte.'}42. The method of claim 41 , wherein the plant is soybean.43. The method of claim 41 , wherein the plant reproductive element is a seed.44. The method of claim 43 , wherein the seed is a transgenic seed.45Streptomyces. The method of claim 41 , wherein the formulation comprises a purified population of the endophyte at a concentration of at least about 10{circumflex over ( )}2 CFU/ml in a liquid formulation or about 10{circumflex over ( )}2 CFU/gm in a non-liquid formulation.46. The method of claim 41 , wherein the formulation further comprises one or more of the following: stabilizer claim 41 , preservative claim 41 , carrier claim 41 , surfactant claim 41 , anticomplex agent claim 41 , ...

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Номер записи: 54

28-06-2018 дата публикации

FUNGAL ENDOPHYTES FOR IMPROVED CROP YIELDS AND PROTECTION FROM PESTS

Номер: US20180177196A1

Автор: Sword Gregory A.

Принадлежит:

Synthetic compositions comprising a plant element and at least one fungal endophyte are described. The fungal endophyte is capable of improving plant tolerance to biotic stress as compared to a reference plant element not further comprising the endophyte. Examples of biotic stress include the biotic stress caused by a nematode, an aphid, a fleahopper, a bug, a stink bug, a soy looper, a cabbage looper, or a fungus. 1. (canceled)2. A synthetic composition , comprising:{'i': 'Purpureocillium', 'a) a fungal endophyte comprising at least one endophyte of the genus ; and'}b) at least one carrier, wherein the fungal endophyte is in contact with the carrier; wherein the fungal endophyte, when heterologously disposed to a plant element, is capable of improving pest resistance as compared to a reference plant element not further comprising the endophyte.3. The synthetic combination of claim 2 , wherein the carrier comprises alginic acid claim 2 , carrageenan claim 2 , dextrin claim 2 , dextran claim 2 , pelgel claim 2 , polyethelene glycol claim 2 , polyvinyl pyrrolidone claim 2 , methyl cellulose claim 2 , polyvinyl alcohol claim 2 , gelatin claim 2 , or combinations thereof.4. The synthetic combination of claim 2 , wherein the composition further comprises water claim 2 , a detergent claim 2 , an insecticide claim 2 , a fungicide claim 2 , or combinations thereof.58-. (canceled)9. The synthetic composition according to claim 1 , wherein the fungal endophyte comprises fungal spores.10. The synthetic composition of claim 9 , comprising about 10 claim 9 , 10 claim 9 , 10 claim 9 , 10 claim 9 , 10 claim 9 , 10 claim 9 , 10 claim 9 , or 10colony forming units per gram or spores per gram.11. The synthetic composition of claim 2 , further comprising a plant element.12. The synthetic composition of claim 11 , wherein the plant element is a dicot.13. The synthetic combination of claim 12 , wherein the dicot is soybean or cotton.14. (canceled)15. The synthetic composition of claim ...

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Номер записи: 55

13-06-2019 дата публикации

ENDOPHYTIC MICROBIAL SEED TREATMENT FORMULATIONS AND METHODS RELATED THERETO FOR IMPROVED PLANT PERFORMANCE

Номер: US20190174692A1

Автор: Cadle-Davidson Molly, HARMAN Gary, Marino Andrea Shelley

Принадлежит: Advanced Biological Marketing, Inc.

This invention describes discovery and development of a biological system of plant growth promotion and environmental improvement by carbon sequestration and/or nitrogen utilization by application of a microbial agent, e.g., a highly effective strain of , particularly strains NRRL B-50520 and/or K5. This strain outperforms the best current strains of used for this purpose available commercially. The highly active products also are expected to increase plant productivity and improve quality of fruits, vegetables, flowers or other plant products. The invention also describes and demonstrates that strain combinations including AS2 or AS3, as well as disclosed metabolites, promote plant growth. This is true across monocots and dicots, seed treatments and foliar sprays. In the field plant height, shoot weight, root weight, and photosynthesis (Spad meter readings) were all increased in comparison to the untreated control as well as the leading biological seed treatments. The invention also describes metabolite utilization for enhancing plant growth. 1. A method of carbon dioxide sequestration from the atmosphere comprising:{'i': Bradyrhizobium', 'Trichoderma', 'Bacillus', 'Pseudomonas', 'Clonastachys, '(a) applying one or more microbial agents to at least one plant, the microbial agent selected from the group consisting of spp., spp., spp., spp. and spp. or any combination thereof wherein the one or more microbial agents are applied to the root cortex of the plant;'}(b) enhancing photosynthetic processes of the at least one plants; and(c) increasing biomass of the root, wherein the carbon dioxide is sequestered due to the increased biomass of the rot.2T. harzianumT. harzianumT. airovirideT. viride, T. viride, T. harzianumT. harzianumT. atrovirideBacillus amloliqofaciens. The method according to claim 1 , wherein the microbial agent is selected from the group consisting of (T22) claim 1 , strain K2 (PTA ATCC 9708) claim 1 , strain K4 (PTA ATCC 9707) claim 1 , strain ...

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Номер записи: 56

15-07-2021 дата публикации

NOVEL METHOD FOR PROMOTING NODULATION IN PLANTS

Номер: US20210212281A1

Автор: Andre Olivier, COMBIER Jean-Philippe

Принадлежит:

Micropeptides (miPEPs), or peptides coded by microRNAs, for promoting nodulation between a plant and a bacterium, as well as their use in this manner. 124-. (canceled)25. A method for promoting nodulation between a plant and a bacterium comprising:(i) providing a plant that naturally expresses an miR comprising a nucleic acid sequence that has at least 80% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 6, wherein the miR regulates the expression of at least one gene involved in the nodulation in said plant and the plant comprises a naturally expressed miPEP that is a peptide of from 3 to 100 amino acids encoded by an open reading frame in the 5′ portion of the primary transcript of the miR, and(ii) exogenously introducing a miPEP into the plant,wherein the miPEP is capable of modulating the accumulation of the miR in said plant and the miPEP has at least 80% identity with the naturally expressed miPEP, and as a peptide chosen among an isolated and/or purified peptide, a synthetic peptide and a recombinant peptide; or', 'as a peptide produced in the plant following the non-natural introduction of a nucleic acid coding said miPEP in said plant, said nucleic acid not comprising the sequence of said miR., 'wherein the miPEP is introduced exogenously either26. The method of claim 25 , wherein the naturally expressed miPEP has at least 80% identity with the amino acid sequence SEQ ID NO: 2 claim 25 , and the miR has a nucleotide sequence consisting of SEQ ID NO: 1.27. The method of claim 25 , wherein the naturally expressed miPEP has at least 80% identity with the amino acid sequence SEQ ID NO: 2.28. The method of claim 25 , wherein the naturally expressed miPEP has at least 80% identity with the amino acid sequence SEQ ID NO: 7 claim 25 , and the miR has a nucleotide sequence consisting of SEQ ID NO: 6.29. The method of claim 25 , wherein the naturally expressed miPEP has at least 80% identity with the amino acid sequence SEQ ID NO: 7.30. The method of claim 25 , ...

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Номер записи: 57

20-06-2019 дата публикации

Endophytes, Associated Compositions, and Methods of Use

Номер: US20190183085A1

Автор: AMBROSE Karen V., Djonovic Slavica, Flavell Richard Bailey, Johnston David Morris, LEFF Jonathan W., Lyford Jeffrey, Marquez Luis Miguel, Millet Yves Alain, Sadowski Craig, SAMAYOA Phillip, Toledo Gerardo V., von Maltzahn Geoffrey, Zhang Xuecheng

Принадлежит:

Materials and methods for improving plant traits and for providing plant benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes. 160.-. (canceled)61Acremonium. A synthetic combination comprising a purified microbial population in association with a plurality of seeds or seedlings of an agricultural plant , wherein the purified microbial population comprises a first endophyte capable of at least one of: production of an auxin , production of a siderophore , and production of acetoin , or a combination of two or more thereof , wherein the first endophyte is of the genus and comprises an ITS rRNA nucleic acid sequence at least 99% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 3605 , 3642 , 3643 , 3644 , 3672 , 3676 , 3650 , 3597 , 3640 , 3602 , or 3647 , and wherein the endophyte is present in the synthetic combination in an amount effective to provide a benefit to the seeds or seedlings or the plants derived from the seeds or seedlings.6267.-. (canceled)68. The synthetic combination of claim 61 , wherein the first endophyte is localized on the surface of the seeds or seedlings.69. The synthetic combination of claim 61 , wherein the first endophyte is obtained from a plant species other than the seeds or seedlings of the synthetic combination.7088.-. (canceled)89. The synthetic combination of claim 61 , wherein the benefit is selected from the group consisting of increased root biomass claim 61 , increased root length claim 61 , increased height claim 61 , increased shoot length claim 61 , increased leaf number claim 61 , increased water use efficiency claim 61 , increased overall biomass claim 61 , increase grain yield claim 61 , increased photosynthesis rate claim 61 , increased tolerance to drought claim 61 , increased heat tolerance claim 61 , increased salt tolerance claim 61 , increased resistance to nematode stress claim 61 , increased resistance to a fungal ...

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Номер записи: 58

04-07-2019 дата публикации

Coniothyrium minitans for use against moss growth

Номер: US20190200619A1

Автор: Sandra Noga

Принадлежит: Bayer CropScience AG

The invention relates to the use of Coniothyrium minitans for controlling moss growth and to a method of controlling moss growth, comprising applying Coniothyrium minitans to an area to be kept free from mosses.

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Номер записи: 59

05-08-2021 дата публикации

A POPULATION GENETICS APPROACH TO BIOLOGICAL CONTROL OF MYCOTOXIN PRODUCTION

Номер: US20210235706A1

Автор: Carbone Ignazio

Принадлежит:

The invention relates to methods for controlling mycotoxin production in field populations of heterothallic and homothallic filamentous fungi through application of biocontrol compositions comprising selected strains of the heterothallic and homothallic filamentous fungi, the selected strains having a lineage of low mycotoxin production or no mycotoxin production and having opposite mating types (heterothallic) or both mating types (homothallic) (e.g., MAT1-1, MAT1-2). 1. A biocontrol method for reducing mycotoxin production in a field population of one or more heterothallic filamentous fungi , the method comprising:applying a biocontrol composition to a plant, a plant part and/or a soil, the biocontrol composition comprising an effective amount of propagules (e.g., colony forming units) of at least two strains of the one or more heterothallic filamentous fungi,wherein the at least two strains comprise a first strain and a second strain of the one or more heterothallic filamentous fungi, each strain comprising a lineage of low mycotoxin production or no mycotoxin production,wherein the first strain further comprises aMAT1-1 mating type and the second strain further comprises aMAT1-2 mating type, andwherein the amount of propagules of the at least two strains of the one or more heterothallic filamentous fungi is effective to promote mating between compatible mating partners in the field population and the at least two strains, thereby increasing the ratio of strains having the lineage of no or low mycotoxin production in the field population of the one or more heterothallic filamentous fungi and reducing mycotoxin production by the field population of the one or more heterothallic filamentous fungi.2. The biocontrol method of claim 1 , wherein the at least two strains of the one or more heterothallic filamentous fungi comprises four strains claim 1 , a first strain claim 1 , a second strain claim 1 , a third strain and a fourth strain claim 1 , each strain comprising ...

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Номер записи: 60

25-06-2020 дата публикации

RE-ENGINEERING OF MYCORRHIZAL SYMBIOSIS IN PLANTS

Номер: US20200199614A1

Автор: Hornstein Eli, Sederoff Heike

Принадлежит:

The invention relates to methods and compositions for modifying naturally non-mycorrhizal plants to produce modified plants that can be colonized by a mycorrhizal fungus having increased nitrogen and phosphorus uptake, increased drought tolerance/resistance, increased resistance to fungal and/or bacterial pathogens, and/or increased growth rate, yield and/or biomass production of a naturally non-mycorrhizal plant. The invention further relates to plants, plant parts, and/or plant cells produced by the methods of the invention as well as harvested and processed products from the plants, plant parts, and/or plant cells. 1. A modified naturally non-mycorrhizal plant , comprising in its genome a heterologous polynucleotide encoding an IPD3 (Interacting Protein of DMI3) polypeptide or a heterologous polynucleotide encoding an IPD3 phosphomimic polypeptide.2. The modified plant of claim 1 , further comprising in its genome a heterologous polynucleotide encoding a DMI3 (Doesn't Make Infections 3) polypeptide or a heterologous polynucleotide encoding a DMI3 phosphomimic polypeptide.3. The modified plant of or claim 1 , further comprising in its genome a heterologous polynucleotide encoding an isoflavone synthase (IFS) polypeptide.4. The modified plant of any one of to claim 1 , further comprising in its genome a heterologous polynucleotide encoding a flavone synthase 1 (FS1) polypeptide and/or a heterologous polynucleotide encoding a flavone synthase 2 (FS2) polypeptide.5. The modified plant of any one of to claim 1 , wherein the plant has increased drought tolerance/resistance claim 1 , increased nitrogen uptake claim 1 , increased phosphorus uptake claim 1 , increased resistance to fungal and/or bacterial pathogens claim 1 , and/or increased growth rate claim 1 , yield and/or biomass production.6. The modified plant of any one of to claim 1 , wherein the naturally non-mycorrhizal plant is a Brassicaceae plant or an Amaranthaceae plant.7CamelinaArabidopsisquinoa. The ...

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Номер записи: 61

03-08-2017 дата публикации

INOCULANTS AND METHODS FOR USE THEREOF

Номер: US20170215358A1

Автор: BALLARD Ross Alexander, FRANCO Christopher Milton Mathew, LE Hoang Xuyen

Принадлежит: THE FLINDERS UNIVERSITY OF SOUTH AUSTRALIA

The present invention relates to methods for enhancing at least one growth parameter of a leguminous plant via co-inoculation of a leguminous plant with at least one rhizobial microorganism together with at least one actinobacterial microorganism. In further aspects, the present invention also relates to leguminous plants co-inoculated with at least one rhizobial microorganism together with at least one actinobacterial microorganism, as well as specific actinobacterial strains and inoculant compositions which are useful in accordance with the present invention. 1. A method for enhancing at least one growth parameter of a leguminous plant , the method comprising co-inoculating the leguminous plant with:at least one rhizobial microorganism; andat least one actinobacterial microorganism;wherein the co-inoculated leguminous plant has at least one enhanced growth parameter relative to a leguminous plant of the same taxon that has not been co-inoculated.2. (canceled)3Streptomyces, MicrobisporaMicromonospora.. The method of wherein the actinobacterial microorganism is from a genus selected from or45.-. (canceled)6. The method of wherein the actinobacterial microorganism comprises a 16S rRNA gene nucleotide sequence which is at least 90% identical to one or more of: SEQ ID NO: 3 claim 1 , SEQ ID NO: 4 claim 1 , SEQ ID NO: 5 claim 1 , SEQ ID NO: 6 claim 1 , SEQ ID NO: 7 claim 1 , SEQ ID NO: 8 claim 1 , SEQ ID NO: 9 or SEQ ID NO: 10.712.- (canceled)13. The method of wherein the growth parameter is a number and/or mass of nodules of the leguminous plant.14. The method of wherein the growth parameter is a number and/or mass of seed pods and/or seed produced by the leguminous plant.15. The method of wherein the growth parameter is a concentration and/or amount of a nutrient in the leguminous plant.16. The method of wherein the nutrient is selected from: Boron claim 15 , Calcium claim 15 , Copper claim 15 , Magnesium claim 15 , Manganese claim 15 , Phosphorous claim 15 , Sodium ...

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Номер записи: 62

04-08-2016 дата публикации

Isolated strain of clonostachys rosea for use as a biological control agent

Номер: US20160222342A1

Автор: John Sutton, Todd Gordon Mason

Принадлежит: Bee Vectoring Technology Inc

Described is an isolated strain of the fungus Colonostachys rosea termed BVT Cr-7 useful as a biological control agent for the treatment of plants. The isolated strain, formulations comprising said strain and/or spores derived from said strain may be applied to plants or plant materials in order to improve plant yield, to improve plant growth, or for the treatment or prevention of diseases or pathogens in the plant.

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Номер записи: 63

20-08-2015 дата публикации

Endophytic Microbial Symbionts in Plant Prenatal Care

Номер: US20150230478A1

Автор: Germida James J., Vujanovic Vladimir

Принадлежит:

The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom. 1. A synthetic preparation comprising an agricultural plant seed and a composition comprising an endophyte capable of promoting germination and an agriculturally-acceptable carrier , wherein an agricultural plant grown from the seed has an altered trait as compared to a control agricultural plant.2. The synthetic preparation of claim 1 , wherein the endophyte capable of promoting germination is a coleorhiza-activating endophyte and the agricultural plant seed is a monocot seed.3FusariumPucciniaRhizobium. The preparation of claim 1 , wherein said composition is disposed on an exterior surface of the agricultural seed in an amount effective to colonize the cortical cells of an agricultural plant grown from the seed and to produce the altered trait claim 1 , wherein the altered trait is an improved functional trait selected from the group consisting of increased yield claim 1 , faster seedling establishment claim 1 , faster growth claim 1 , increased drought tolerance claim 1 , increased heat tolerance claim 1 , increased cold tolerance claim 1 , increased salt tolerance claim 1 , increased tolerance to infection claim 1 , increased tolerance to infection claim 1 , increased biomass claim 1 , increased root length claim 1 , increased fresh weight of seedlings claim 1 , increased plant vigor claim 1 , nitrogen stress tolerance claim 1 , enhanced activity claim 1 , enhanced nodulation frequency claim 1 , and early flowering time.4. The preparation of claim 3 , wherein said composition is disposed on an exterior surface ...

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Номер записи: 64

30-10-2014 дата публикации

Methods for controlling leaf-cutting ants

Номер: US20140322339A1

Автор: Daniela Goffre, Jorge Ariel Marfetan, Patricia Folgarait

Принадлежит: Universidad Nacional de Quilmes

Methods and compositions for controlling leaf-cutting ants. More particularly, methods and kits for controlling leaf-cutting ants by serially supplying different formulations, and a method for increasing production of conidia of the Escovopsis fungus useful as controlling agent in the methods and compositions are disclosed herein. The methods comprise serially supplying at least two bait formulations, each formulation containing at least one controlling agent for the ants and one attractant and/or controlling agent masking substance, wherein the attractants and/or controlling agent masking substance are different on each formulation.

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Номер записи: 65

10-09-2015 дата публикации

METHODS OF SCREENIING FOR MICROORGANISMS THAT IMPART BENEFICIAL PROPERTIES TO PLANTS

Номер: US20150250116A1

Автор: GEORGE Caroline Elizabeth, Turner Susan Jane, Wigley Peter John

Принадлежит:

The present invention relates to methods for the screening, identification and/or application of microorganisms and/or compositions of use in imparting beneficial properties to plants, and microorganisms and compositions identified therefrom. 1. A method for the selection of one or more microorganisms capable of imparting one or more beneficial property to a plant , the method comprising at least the steps of:a) subjecting one or more plant (including seeds, seedlings, cuttings, and/or propagules thereof) to a growth medium in the presence of a first set of one or more microorganisms;b) selecting one or more plant following step a);c) acquiring a second set of one or more microorganisms from said one or more plant selected in step b);d) repeating steps a) to c) one or more times, wherein the second set of one or more microorganisms acquired in step c) is used as the first set of microorganisms in step a) of any successive repeat.2. A method according to claim 1 , wherein the second set of one or more microorganisms are isolated from said one or more plant in step c).3. A method according to or claim 1 , wherein the step of subjecting one or more plant to a growth medium involves growing or multiplying the plant.4. A method according to any one of to wherein two or more claim 1 , 10 or more claim 1 , 20 or more claim 1 , 100 or more claim 1 , 300 or more claim 1 , 500 or more claim 1 , or 1000 or more plants are subjected to a growth medium in the presence of the first set of microorganisms.5. A method according to any one of to claim 1 , wherein the one or more plant is selected on the basis of one or more selection criterion.6. A method according to claim 5 , wherein the one or more plant is selected on the basis of one or more phenotypic trait.7. A method according to claim 5 , wherein the one or more plant is selected on the basis of one or more genotypic trait.8. A method according to claim 5 , wherein the one or more plant is selected based on a combination of ...

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Номер записи: 66

15-09-2016 дата публикации

IMPROVED FUNGAL ENDOPHYTES

Номер: US20160262335A1

Автор: CARD Stuart Douglas, HUME David Edward, JOHNSON Richard David, SIMPSON Wayne Roydon

Принадлежит:

The present invention relates to at least one epichloae fungal endophyte strain combined with at least one species of spp., which confers at least some level of pest protection on the spp. plant. In particular, the invention relates to an isolated strain of epichloae fungal endophyte selected from the group consisting of AR3039 (NRRL #50716), AR3046 (NRRL #50576), AR3049 (NRRL #50577), AR3050 (NRRL #50578), AR3064 (NRRL #50718), AR3067 (NRRL #50719), AR3068 (NRRL #50720), AR3073 (NRRL #50721), AR3074 (NRRL #50722), AR3076 (NRRL #50723), and AR3078 (NRRL #50724), and combinations thereof; a spp. plant infected with a fungal endophyte wherein spp. is not a natural host of the endophyte; a method of making a stable host plant/epichloae fungal endophyte combination; a method of conferring at least some level of pest protection on a host spp. plant; and a spp. seed infected with an epichloae fungal endophyte. 167-. (canceled)68SecaleSecale. A spp. plant infected with an epichloal fungal endophyte wherein spp. is not a natural host of the endophyte , wherein the epichloae fungal endophyte is selected from the group consisting of AR3039 (NRRL #50716) , AR3046 (NRRL #50576) , AR3049 (NRRL #50577) , AR3050 (NRRL #50578) , AR3064 (NRRL #50718) , AR3067 (NRRL #50719) , AR3068 (NRRL #50720) , AR3073 (NRRL #50721) , AR3074 (NRRL #50722) , AR3076 (NRRL #50723) , and AR3078 (NRRL #50724) , or combinations thereof , and wherein the plant and endophyte form a stable symbiotic association that allows the plant to progress through a normal life cycle.69Secale. A spp. plant according to claim 68 , wherein the epichloal fungal endophyte is selected from the group consisting of AR3039 (NRRL #50716) claim 68 , AR3046 (NRRL #50576) claim 68 , AR3050 (NRRL #50578) claim 68 , AR3067 (NRRL #50719) claim 68 , AR3068 (NRRL #50720) claim 68 , AR3074 (NRRL #50722) claim 68 , and AR3078 (NRRL #50724).70Secale. A spp. plant according to claim 68 , wherein the epichloal fungal endophyte is selected ...

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Номер записи: 67

06-08-2020 дата публикации

NEW BIO-PESTICIDES FOR CONTROLLING PLANT PESTS

Номер: US20200245628A1

Автор: BUTALA Matej, GRUNDNER Maja, HODNIK Vesna, MACEK Peter, MODIC Spela, NOVAK Marusa, PANEVSKA Anastasija, RAZINGER Jaka, SEPCIC Kristina, SKOCAJ Matej, TURK Tom

Принадлежит:

The present invention relates to cytolytic bi-component protein complexes consisting of a plurality of molecules of a member of the aegerolysin family and a plurality of molecules of a member of the MACPF superfamily, and particularly to their use for controlling a plant pest, such as for controlling Colorado potato beetle () or Western corn rootworm (). More specifically, the invention relates to cytolytic bi-component protein complexes formed by a plurality of molecules of one of the aegerolysins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) with a plurality of molecules of pleurotolysin B (PlyB) or similar proteins, which have been shown to be toxic for the aforementioned agricultural pest insects. 132-. (canceled)33. A method for protecting a plant against a plant pest and/or controlling or treating a plant pest , comprising the step of: applying a composition comprising a plurality of molecules of a member of the aegerolysin family , a plurality of molecules of a member of the MACPF superfamily and a suitable carrier to a plant in need thereof.34. (canceled)35Pleurotus.. The method according to claim 33 , wherein the member of the aegerolysin family is an aegerolysin derived from a fungus of the genus36. The method according to claim 33 , wherein the member of the aegerolysin family is selected from the group consisting of ostreolysin claim 33 , pleurotolysin A and erylysin A.37. The method according to claim 33 , wherein the member of the aegerolysin family is ostreolysin.38. The method according to claim 37 , wherein the ostreolysin is a polypeptide comprising an amino acid sequence having at least 50% sequence identity with SEQ ID NO: 1.39. The method according to claim 33 , wherein the member of the aegerolysin family is pleurotolysin A.40. The method according to claim 39 , wherein the pleurotolysin A is a polypeptide comprising an amino acid sequence having at least 50% sequence identity with SEQ ID NO: 2.41. The method according ...

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Номер записи: 68

01-10-2015 дата публикации

Compacted pesticide formulations

Номер: US20150272129A1

Автор: Miron Teshler, Silvia Ivanova Todorova

Принадлежит: Individual

It is herewith provided a pesticidal composition composing a microbial propaguse (such as a conidia from B. bassiana ) and an agriculturally-acceptable carrier (such as kaolin day) provided in a solid formulation obtained by dry compaction. The pesticidal composition can optionally contain a water dispersant (such as starch) as well as a binder (such as xhantam gum). The pesticidal composition doss not contain a surfactant but is nevertheless dispersible in water.

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Номер записи: 69

13-08-2020 дата публикации

Method for preparing biopesticide preparation for preventing and controlling solenopsis invicta

Номер: US20200253216A1

Автор: Jingwen YE, Jun Li, Shichou Han, Xin Lv, Yuke YA

Принадлежит: GUANGDONG INSTITUTE OF APPLIED BIOLOGICAL RESOURCES

A method for preparing a biopesticide preparation for preventing and controlling Solenopsis invicta comprising: a: preparing an agent containing pathogenic microorganisms for preventing and controlling Solenopsis invicia, the agent comprises Beauveria bassiana and sodium chloride, with a Beauveria bassiana spores to sodium chloride ratio of (1-8)×20 billion spores: 4.0-7.5 g; b: placing the agent into a solid container, and adding a corresponding amount of solution in a liquid container; inserting the solid container into the liquid container, wherein a retaining ring is stuck at the opening of the liquid container, a rubber gasket is pressed against the retaining ring, and a lid covers the rubber gasket and the retaining ring and is fixed to the opening of the liquid container.

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Номер записи: 70

13-08-2020 дата публикации

METHODS FOR ENHANCED ROOT NODULATION IN LEGUMES

Номер: US20200253217A1

Автор: BALDRIDGE John W., GOLDFELD Michael G., MICHALOW Andrew H., PODELLA Carl W.

Принадлежит: ADVANCED BIOCATALYTICS CORPORATION

Disclosed herein are methods of increasing, enhancing, or accelerating root nodulation in a plant, accelerating growth of nitrogen fixing bacteria in nodules of a plant, increasing protein content in a plant, increasing yield of a plant, improving water retention of a plant, or reducing water use of a plant, the method comprising identifying a plant in need of root nodulation, and applying to the plant a composition comprising a protein component comprising yeast stress proteins resulting from subjecting a mixture obtained from the yeast fermentation to stress. 1. A method of increasing , enhancing , or accelerating root nodulation in a plant , accelerating growth of nitrogen fixing bacteria in nodules of a plant , increasing protein content in a plant , increasing yield of a plant , improving water retention of a plant , or reducing water use of a plant , the method comprisingidentifying a plant in need of root nodulation, andapplying to the plant a composition comprising a protein component comprising yeast stress proteins resulting from subjecting a mixture obtained from the yeast fermentation to stress.2. The method of claim 1 , wherein the composition is applied to the soil near the plant.3. The method of claim 1 , wherein the composition is applied through irrigation.4. The method of claim 3 , wherein the irrigation is spray irrigation or drip irrigation.5. The method of claim 1 , wherein the composition is applied with every watering cycle or intermittent basis.6. The method of claim 1 , wherein the protein component is from aerobic fermentation of yeast.7. The method of claim 1 , wherein the protein component comprises proteins obtained from exposing a product obtained from the fermentation of yeast to additional procedures that increase the yield of proteins produced from the fermentation.8. The method of claim 1 , wherein the stress is selected from the group consisting of heat shock of the fermentation product claim 1 , physical and/or chemical disruption ...

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Номер записи: 71

08-10-2015 дата публикации

Composition comprising a biological control agent and a fungicide

Номер: US20150282490A1

Автор: Haruko Sawada, Peter Dahmen, Ulrike Wachendorff-Neumann

Принадлежит: Bayer CropScience AG

The present invention relates to a composition comprising at least one biological control agent selected from the group consisting of Paecilomyces lilacinus strain 251 (AGAL No. 89/030550) and Coniothyrium minitans CON/M/91-08 (DSM 9660) and/or a mutant of these strains having all the identifying characteristics of the respective strain, and/or at least one metabolite produced by the respective strain that exhibits activity against nematodes, insects and/or phytopathogens, and at least one fungicide (I) selected from the group consisting of inhibitors of the ergosterol biosynthesis in a synergistically effective amount. Furthermore, the present invention relates to a kit of parts comprising said composition and the use of said composition.

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Номер записи: 72

28-09-2017 дата публикации

A method for improving the mean dry shoot weight, mean dry grain weight, and suppressing seed-borne infection in a cereal crop

Номер: US20170273271A1

Автор: Brian Murphy, Fiona Doohan, Trevor HODKINSON

Принадлежит: College of the Holy and Undivided Trinity of Queen Elizabeth near Dublin, UNIVERSITY COLLEGE DUBLIN

A seed coating composition comprising a mixture of a fungal root endophyte isolated from a root of the plant found in low-nutrient, drought-stressed or multiply-stressed conditions and a carrier medium for application of said composition to a target seed, wherein the endophyte is characterised in having a nuclear ribosomal internal transcribed spacer (nr ITS) with at least a 90% sequence identity to any one of SEQ ID NOs: 3 to 15.

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Номер записи: 73

06-10-2016 дата публикации

ACCELERATED DIRECTED EVOLUTION OF MICROBIAL CONSORTIA FOR THE DEVELOPMENT OF DESIRABLE PLANT PHENOTYPIC TRAITS

Номер: US20160289667A1

Автор: GEORGE Caroline, TURNER Susan, Wigley Peter

Принадлежит: Biodiscovery New Zealand Limited

The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants. 2. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the first and/or second microbial community to an element or condition that is selective for the survival of spore-forming microbes and is deleterious to the survival of microbes that do not form spores.3. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the first and/or second microbial community to a solvent or sterilant that is selective for the survival of spore-forming microbes and is deleterious to the survival of microbes that do not form spores.4. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the first and/or second microbial community to an organic solvent that is selective for the survival of spore-forming microbes and is deleterious to the survival of microbes that do not form spores.5. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the first and/or second microbial community to an alcohol that is selective for the survival of spore-forming microbes and is deleterious to the survival of microbes that do not form spores.6. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the first microbial community of step a) to an alcohol.7. The method according to claim 1 , wherein the enrichment procedure that ensures the selective survival of spore-forming microbes comprises: exposing the second microbial community of step c) to ...

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Номер записи: 74

27-08-2020 дата публикации

Metabolite Production in Endophytes

Номер: US20200270314A1

Автор: BROHIER Natasha Denise, DAVIDSON Sophie Elizabeth, Guthridge Kathryn Michaela, HETTIARACHCHIGE Inoka Kumari, Ludlow Emma Jane Isobel, Mann Ross, Rochfort Simone Jane, Sawbridge Timothy Ivor, Spangenberg German Carlos, VASSILIADIS Simone

Принадлежит: AGRICULTURE VICTORIA SERVICES PTY LTD

The present invention relates to nucleic acids encoding amino acid sequences for the biosynthesis of janthitrem in janthitrem producing endophytes. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides, regulatory elements and methods. 116-. (canceled)17. A substantially purified or isolated nucleic acid or nucleic acid fragment encoding a janthitrem biosynthesis polypeptide , or complementary or antisense to a sequence encoding a janthitrem biosynthesis polypeptide , said nucleic acid or nucleic acid fragment including a nucleotide sequence selected from the group consisting of (a) sequences shown in (Sequence ID Nos 1 , 2 , 5 , 6 , 9 , 10 , 13 and 14); (b) complements of the sequences in (a); (c) sequences antisense to the sequences recited in (a) and (b); and (d) functionally active fragments and variants of the sequences recited in (a) , (b) and (c) having at least approximately 80% identity to the relevant part of the sequences recited in (a) , (b) and (c) and having a size of at least 20 nucleotides.18. A nucleic acid or nucleic acid fragment according to claim 17 , wherein said functionally active fragments and variants have at least approximately 95% identity to the relevant part of the sequences recited in (a) claim 17 , (b) and (c) and have a size of at least 200 nucleotides.19. A nucleic acid or nucleic acid fragment according to claim 18 , wherein said nucleic acid or nucleic acid fragment includes a nucleotide sequence shown in .20. A construct including a nucleic acid or nucleic acid fragment according to .21. A construct according to claim 20 , wherein said construct is a vector further including a promoter and a terminator claim 20 , said promoter claim 20 , nucleic acid or nucleic acid fragment and terminator being operatively linked.22. A method of modifying janthitrem biosynthesis in an endophyte claim 20 , said method including introducing into said endophyte an effective amount of a ...

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Номер записи: 75

03-09-2020 дата публикации

PREPARATION COMPRISING AT LEAST ONE CHEMICAL FUNGICIDE AND A MIXTURE COMPRISING AUREOBASIDIUM PULLULANS STRAINS

Номер: US20200275665A1

Автор: BINDER Eva-Maria, DONAT Christina Maria

Принадлежит: Erber Aktiengesellschaft

The invention relates to a preparation comprising at least one chemical fungicide. In addition to the at least one chemical fungicide, a mixture is included which comprises at least the strains of DMS 14940 and DSM 14941. The at least one chemical fungicide and the mixture of the strains are present in the preparation in synergistically active quantities, and the at least one chemical fungicide is selected from fludioxinil or a combination containing fludioxinil and at least one second chemical fungicide. The invention also relates to the use of the preparation and to a method for the prophylaxis or the reduction of the spread of at least one plant disease caused by a fungal pathogen. 1Aureobasidium pullulansAureobasidium pullulans. A preparation comprising at least one chemical fungicide , characterized in that , in addition to the at least one chemical fungicide , a mixture comprising at least the strains DSM 14940 and DSM 14941 is present , the at least one chemical fungicide and the mixture of the strains being present in the preparation in synergistically effective amounts , and that the at least one chemical fungicide is selected from fludioxonil or a combination comprising fludioxonil and cyprodinil.2Aureobasidium pullulans. The preparation according to claim 1 , wherein the strains DSM 14940 and DSM 14941 are present in the preparation in a cell quantity ratio of 2:1 to 1:2.3Aureobasidium pullulans. The preparation according to claim 1 , wherein the strains DSM 14940 and DSM 14941 are each present in a concentration of 1×10to 1×10cells/ml preparation as synergistically effective amounts.4. The preparation according to claim 1 , wherein cyprodinil is present in a concentration of 0.00375 g/l preparation to 5 g/l preparation as a synergistically effective amount.5. The preparation according to claim 1 , wherein fludioxonil is present in a concentration of 0.0025 g/l preparation to 5 g/l preparation as a synergistically effective amount.6. The preparation ...

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Номер записи: 76

10-09-2020 дата публикации

FUSARIUM SOLANI AND USE OF SAME IN PREVENTION AND TREATMENT OF SOUTHERN BLIGHT OF DENDROBIUM OFFICINALE KIMURA ET MIGO

Номер: US20200281214A1

Автор: Shi Jun, XI Gangjun, Xu Chao, Yang Hetong, ZHANG Hongyan, Zheng Kai

Принадлежит:

A Fusarium solani strain and a use thereof in the control of southern blight disease of Dendrobium officinale Kimura et Migo, and the strain JSNL007-2 has been identified as Fusarium solani and has been deposited in China Center for Type Culture Collection on Mar. 10, 2017 with a deposit number of CGMCC NO. 13687. The Fusarium solani JSNL007-2 of the present invention has a significant effect on the disease resistance of Dendrobium officinale Kimura et Migo, especially for improving the ability of Dendrobium officinale Kimura et Migo to resist southern blight disease, and has a certain effect on reducing the use of chemical pesticides, which is an important means to ensure food safety. 1. A Fusarium solani strain JSNL007-2 , which has been deposited in China Center for Type Culture Collection on Mar. 10 , 2017 with a deposit number of CGMCC NO. 13687.2. The Fusarium solani strain JSNL007-2 according to claim 1 , wherein the Fusarium solani strain JSNL007-2 is used for controlling a southern blight disease of Dendrobium officinale Kimura et Migo by administrating the Fusarium solani strain JSNL007-2 to a subject needed for controlling the southern blight disease of Dendrobium officinale Kimura et Migo.3. A microbial inoculant comprising the Fusarium solani strain JSNL007-2 of .4. The microbial inoculant according to claim 3 , wherein the microbial inoculant is prepared by the following steps:(1) preparing a solid medium using cottonseed husk dregs as culture substrate, ammonium nitrate as exogenous nitrogen source, and sucrose as exogenous carbon source, sterilizing, and inoculating the Fusarium solani strain JSNL007-2;(2) culturing for 5-8 days under a condition of 25-30° C., 8 hours of light and 16 hours of darkness per day, and pH of 6.0-6.5;{'sup': '3', '(3) cutting the solid microbial inoculant cultured in step (2) into 1-1.5 cmblocks and setting aside.'}5. The microbial inoculant according to claim 4 , wherein the sterilizing condition in step (1) is ...

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Номер записи: 77

17-09-2020 дата публикации

METHOD OF IMPROVING STORAGE STABILITY AND FITNESS OF FUNGAL SPORES

Номер: US20200288723A1

Автор: LANVER Daniel, Neumann Fred, SCHINK Stefan, STEINORT Tim

Принадлежит:

The present invention relates to a method for producing dormant fungal structures or organs with an improved germination rate comprising subjecting said dormant structures or organs to a procedure comprising a heat treatment, followed by a cooling period as well as a related solid-state fermentation method and dormant fungal structures or organs produced thereby.

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Номер записи: 78

03-11-2016 дата публикации

Chitooligosaccharides and methods for use in enhancing corn growth

Номер: US20160316749A1

Автор: Ahsan Habib, R. Stewart Smith

Принадлежит: Novozymes Bioag As

Disclosed are methods of enhancing growth of corn plants, comprising treating corn seed or the corn plant that germinates from the seed with an effective amount of at least one chitooligosaccharide, wherein upon harvesting the corn plant exhibits at least one of increased plant yield measured in terms of bushels/acre, increased root number, increased root length, increased root mass, increased root volume and increased leaf area, compared to untreated corn plants or corn plants harvested from untreated corn seed.

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Номер записи: 79

12-11-2015 дата публикации

Composition comprising a biological control agent and a fungicide

Номер: US20150320051A1

Автор: Haruko Sawada, Peter Dahmen, Ulrike Wachendorff-Neumann

Принадлежит: Bayer CropScience AG

The present invention relates to a composition comprising at least one biological control agent selected from the group consisting of Paecilomyces lilacinus strain 251 (AGAL No. 89/030550) and Coniothyrium minitans CON/M/91-08 (DSM 9660) and/or a mutant of these strains having all the identifying characteristics of the respective strain, and/or at least one metabolite produced by the respective strain that exhibits activity against nematodes, insects and/or phytopathogens, and at least one fungicide (I) selected from the group consisting of inhibitors of the respiratory chain at complex I, II and III in a synergistically effective amount. Furthermore, the present invention relates to a kit of parts comprising said composition and the use of said composition.

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Номер записи: 80

24-09-2020 дата публикации

FUNGAL IMMUNOGENS AND RELATED MATERIALS AND METHODS

Номер: US20200297826A9

Автор: Narra Hema P., Orbach Marc J., Shubitz Lisa F.

Принадлежит: Arizona Board of Regents on Behalf of the University of Arizona

The present disclosure provides immunogenic materials and methods useful for reducing the risk of fungal infections, particularly valley fever. The disclosure also provides assays for identifying compounds useful to treat valley fever, as well as methods for ameliorating the symptoms of valley fever. 143-. (canceled)44. An immunogenic vaccine composition , comprising:{'i': 'Coccidioides', 'An avirulent arthroconidia comprising a genetically modified or missing cyclic peptide synthase (CPS1) protein; and'}a pharmaceutically acceptable carrier.45. The immunogenic vaccine composition of claim 44 , wherein the composition is formulated for human administration.46. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for intranasal administration.47. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for subcutaneous administration.48. The immunogenic vaccine composition of claim 44 , wherein the vaccine composition is formulated for intramuscular administration.49. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of at least a portion of the CPS1 gene encoding the CPS1 protein.50. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of the DNA methyltransferase-associated protein (DMAP) binding domain region of the CPS1 gene encoding the CPS1 protein.51. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing by deletion of the adenosine monophosphate (AMP) binding domain region of the CPS1 gene encoding the CPS1 protein.52. The immunogenic vaccine composition of claim 44 , wherein the CPS1 protein is genetically modified or missing as a result of a deletion of at least one regulatory element of the CPS1 gene encoding the CPS1 protein.53. The immunogenic vaccine composition of claim 44 , ...

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Номер записи: 81

01-10-2020 дата публикации

METHOD FOR INDUCING ACTIVE RESPONSE OF PLANTS

Номер: US20200305374A1

Автор: CI Lijie, Fang Xiang, GUO Zhijiang, JIN Gong, LI Zhian, TU Jiangping, Xie Jianping, ZUO LIGANG

Принадлежит: BEIJING XINNA INTERNATIONAL NEW MATERIALS TECHNOLOGY CO., LTD.

The present invention provides a method, which is used with trace amount, can initiate an active endogenous response of plants, and can obtain expected effects such as growth acceleration and yield increase of the plants. The present invention further provides preparation of a signal carrier material for implementing the method. In the method, by utilizing genetic information stored in the plants, the plants can adapt to an environment through a signal which makes the plants mistakenly perceive, thereby achieving an expected objective. The method is suitable for planting of crops in regions with poor natural conditions or severe climate changes. 1. A method for inducing an active response of plants , characterized in that the method uses a signal carrier material as an exogenous signal carrier for the plants by utilizing genetic information stored in an early evolution process of the plants , and artificially creating an environmental condition that makes the plants mistake for a growth acceleration requirement;the plants perceive an exogenous signal carried by the exogenous signal carrier by sensing or capturing the exogenous signal carrier, so that the plants perceive an environment change to accelerate growth and reproduction, and make an active response to the perception.2. The method for inducing the active response of the plants according to claim 1 , characterized in that the active response to the exogenous signal perceived by the plants is manifested by a detected targeted parameter through an observation claim 1 , or a genetic claim 1 , physiological claim 1 , cellular or phenotypic detection method.3. The method for inducing the active response of the plants according to claim 1 , characterized in that the active response to the exogenous signal perceived by the plants is embodied in a detected change in an expression level of an endogenous hormone in signal transduction in plants.4. The method for inducing the active response of the plants according to ...

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Номер записи: 82

01-10-2020 дата публикации

NOVEL STRAIN OF BEAUVERIA BASSIANA, CAPABLE OF INFECTING HORNETS

Номер: US20200305439A1

Автор: LABORIE Bénédicte, POIDATZ Juliette, Thiery Denis

Принадлежит:

The present invention relates to a novel strain of the entomopathogenic fungus which presents the specificity of being able to infect the Asian hornet, 1Beauveria bassiana,Vespa. A strain of the fungus wherein a sample of said strain has been deposited at the Collection Nationale de Cultures des Microorganismes (CNCM) under registration number CNCM I-5254 , or a mutant thereof having the capacity to infect hornet species of the genus sp.2. Spores of the strain according to .3. A composition comprising a biologically-pure culture of the strain according to or the spores according to .4. A composition comprising fermentation products of the strain according to .5. A method for treating the nests of hornets with the strain claim 1 , its spores claim 1 , or the composition according to any of to claim 1 , comprising spraying the strain claim 1 , its spores claim 1 , and/or the composition on and/or inside the nests with any suitable spraying means.6. The method according to claim 5 , characterized in that the strain or the composition is applied into the nests.7. The method according to claim 6 , characterized in that the method additionally comprises a preliminary step of perforating the nest with any means suitable for perforating the nests.8. The method according to claim 7 , characterized in that the means suitable for perforating the nests is the suitable spraying means.9. A method for contaminating a nest of hornets claim 7 , characterized in that (i) at least one hornet is captured claim 7 , (ii) a certain amount of the strain claim 7 , its spores claim 7 , or the compositions according to any of to is deposited on its body claim 7 , and (iii) the hornet is released and allowed to return to its nest.10. A device comprising means that are suitable for depositing a certain amount of the strain claim 7 , its spores claim 7 , or composition according to any of to on the body of hornets.11. A food composition that is attractive for hornets to feed on claim 7 , ...

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Номер записи: 83

08-11-2018 дата публикации

MICROBE ASSOCIATIONS THAT HAVE NEW OR IMPROVED CHARACTERISTICS

Номер: US20180320242A1

Автор: Furlan Michael A., Ghodsalavi Behnoushsadat, Nicolaisen Mette, Nybro Ole, Olsson Stefan, Springer Deborah Jean

Принадлежит: University of Copenhagen

Disclosed are methods for identifying microbes that associate with other microbes, and possess new or improved characteristics. Also disclosed are microbes isolated using the methods. Also disclosed are methods for using the microbes. 1. A method , comprising:enriching a population of microbes in a soil sample for bacteria that associate with a non-mycorrhizal fungus that is added to the soil sample;testing the bacteria that associate with the fungus, for bacteria that affect growth of the fungus; andplacing the bacteria that affect growth of the fungus together with the non-mycorrhizal fungus to form a mixture, and screening the mixture for a characteristic not present in either the bacteria or non-mycorrhizal fungus alone, or for improvement in a characteristic present in either the bacteria or non-mycorrhizal fungus alone.2. The method of claim 1 , where the bacteria that affect growth of the fungus claim 1 , stimulate growth of the fungus.35-. (canceled)6. A method claim 1 , comprising:{'i': 'Penicillium bilaiae', 'enriching a population of microbes in a soil sample for bacteria that associate with a hyphae of a that is added to the soil sample;'}{'i': 'Penicillium bilaiae', 'testing the bacteria that associate with the hyphae, for bacteria that stimulate growth of the ; and'}{'i': Penicillium bilaiae', 'Penicillium bilaiae., 'screening the bacteria that stimulate growth of the for the capability to increase an amount of phosphate solubilized by the'}7. (canceled)8Penicillium bilaiaePenicillium bilaiae. The method of claim 6 , where the testing includes placing the bacteria and the on a support so they do not initially contact one another and claim 6 , after a period of time claim 6 , determining whether the bacteria and the contact each other on the support.9. The method of claim 8 , where the testing uses a microbial medium that does not contain nutrients in addition to nutrients supplied by bacteriological-grade agar at a concentration between about 0.5-1.5%. ...

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Номер записи: 84

16-11-2017 дата публикации

Isolated strain of clonostachys rosea for use as a biological control agent

Номер: US20170327784A1

Автор: John Sutton, Todd Gordon Mason

Принадлежит: Bee Vectoring Technology Inc

Described is an isolated strain of the fungus Colonostachys rosea termed BVT Cr-7 useful as a biological control agent for the treatment of plants. The isolated strain, formulations comprising said strain and/or spores derived from said strain may be applied to plants or plant materials in order to improve plant yield, to improve plant growth, or for the treatment or prevention of diseases or pathogens in the plant.

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Номер записи: 85

24-10-2019 дата публикации

ENDOPHYTIC MICROBIAL SYMBIONTS IN PLANT PRENATAL CARE

Номер: US20190320610A1

Автор: Germida James J., Vujanovic Vladimir

Принадлежит:

The present disclosure provides novel endophyte strains or cultures thereof that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom. 153.-. (canceled)54Streptomyces. A plant seed manually or mechanically coated with at least one endophyte , wherein the plant is a cereal that is not wheat and the endophyte is a sp. strain or culture thereof which is deposited under IDAC 081111-06 or which comprises the 16S rDNA sequence as shown in SEQ ID NO:6.55. The coated plant seed of claim 54 , wherein the plant is a barley plant.56. A method of improving seed vitality claim 54 , plant health and/or yield comprising cultivating the coated plant seed of into a first generation plant.57. The method of claim 56 , wherein the plant is a barley plant.58RhizobiumFusarium. The method of claim 56 , wherein the improving seed vitality claim 56 , plant health claim 56 , and/or yield is for increasing seed germination claim 56 , for decreasing time to reach the energy of germination claim 56 , for reducing hydrothermal time required for germination claim 56 , for increasing seed germination vigour claim 56 , for increasing the fresh weight of seedlings claim 56 , for enhancing nodulation traits claim 56 , for increasing yield of seedlings claim 56 , for reducing the effects of stress on the seed or cultivated plant claim 56 , for reducing the effects of drought claim 56 , heat and/or biotic stress on the seed or cultivated plant claim 56 , for reducing a infection on the seed or cultivated plant claim 56 , for enhancing stratification claim 56 , for breaking dormancy claim 56 , for reducing the effects of stress by modulating gene expression of hormonal ent-kaurenoic (KAO) claim 56 , for repression of shoot ...

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Номер записи: 86

15-10-2020 дата публикации

ACCELERATED DIRECTED EVOLUTION OF MICROBIAL CONSORTIA FOR THE DEVELOPMENT OF DESIRABLE PLANT PHENOTYPIC TRAITS

Номер: US20200325470A1

Автор: GEORGE Caroline, TURNER Susan, Wigley Peter

Принадлежит:

The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants. 161-. (canceled)62. A process for producing a microbial product that helps to promote the development of a beneficial phenotypic plant trait , comprising:a) growing a plurality of plants in a growth medium, in the presence of a first microbial community;b) selecting at least one plant from said plurality, following step a), based upon a beneficial phenotypic trait exhibited by the plant relative to other plants of said plurality;c) acquiring a second microbial community from the at least one plant selected in step b);d) repeating steps a) to c) in an iterative manner, wherein the second microbial community acquired in step c) is used as an inoculum in step a) of any successive repeat; ande) acquiring a microbial community that is associated with promoting the development of a beneficial phenotypic plant trait; andf) producing a microbial product by combining at least one microorganism from said microbial community into a microbial product.63. The method according to claim 62 , comprising: isolating at least one microorganism from the microbial community acquired in step e).64. The method according to claim 62 , comprising: isolating at least one microorganism from the microbial community acquired in step e) and utilizing a molecular technique to characterize the isolated microbe.65. The method according to claim 62 , wherein the microbial product is formulated as a liquid.66. The method according to claim 62 , wherein the microbial product is formulated as a solid.67. The method according to claim 62 , wherein the microbial product is formulated as a seed coating.68. The method according to claim 62 , wherein the microbial product is formulated for application onto a growing plant.69. The method according to claim 62 , wherein the microbial product is formulated for ...

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Номер записи: 87

07-12-2017 дата публикации

FUNGAL CHROMOSOME-END KNOCKOFF STRATEGY

Номер: US20170349899A1

Автор: Farman Mark L., Florea Simona, Schardl Christopher L.

Принадлежит:

Non-toxigenic fungal strains, and methods of making and use thereof, are provided and have utility as endophytes in forage crops, and as strains that can outcompete toxigenic strains in forage and food crops. 1. An isolated nucleic acid molecule , comprising:(a) a marker gene;(b) a series of telomere repeats, wherein each telomere repeat has the nucleic acid sequence of a telomere repeat selected from SEQ ID NOs: 31-54, wherein the series includes about 3 to about 26 telomere repeats; and(c) a recombination sequence comprising a variant or fragment of a wild type gene of a gene cluster selected from: an ergot alkaloid gene cluster EAS1, an ergot alkaloid gene cluster EAS2, an indole-diterpene gene cluster (IDT), and aflatoxin gene clusters.2. The nucleic acid molecule of claim 1 , wherein the gene of an ergot alkaloid gene cluster is lpsA1.3. The nucleic acid molecule of claim 1 , wherein the recombination sequence comprises SEQ ID NO:3.4. The nucleic acid molecule of wherein the marker gene is selected from hph claim 1 , neomycin phosphotransferase II gene claim 1 , phleomycin resistance gene claim 1 , and bialophos resistance gene.5. A vector claim 1 , comprising the isolated nucleic acid molecule of .6EpichloëEpichloë festucae,Epichloë festucae×Epichloë typhinaEpichloë coenophiala.. The vector of claim 5 , wherein the variant or fragment of the wild type gene is from an species claim 5 , the species comprising an hybrid claim 5 , or7. A kit claim 5 , comprising the vector of .8EpichloëAspergillus. A method for eliminating some or substantially all of one or more target genes located in a subterminal region of a chromosome in a fungus selected from an species and an species claim 5 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'integrating a nucleic acid molecule of into the genome of the fungus by homologous recombination, thereby replacing the one or more target genes with the nucleic acid molecule, wherein the series of telomere repeats will ...

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Номер записи: 88

07-11-2019 дата публикации

DESIGNED COMPLEX ENDOPHYTE COMPOSITIONS AND METHODS FOR IMPROVED PLANT TRAITS

Номер: US20190335759A1

Автор: AMBROSE Karen V., Boghigian Brett A., Djonovic Slavica, Gray Paul Andrew, Marquez Luis Miguel, Pelaez Julianne Naomi, Toledo Gerardo V., von Maltzahn Geoffrey

Принадлежит:

This invention relates to methods and materials for providing a benefit to a plant by associating the plant with a complex endophyte comprising a host fungus further comprising a component bacterium, including benefits to a plant derived from a seed or other plant element treated with a complex endophyte. For example, this invention provides purified complex endophytes, purified complex endophyte components such as bacteria or fungi, synthetic combinations comprising said complex endophytes and/or components, and methods of making and using the same. 1118.-. (canceled)119. A method of improving a trait of agronomic importance in a plant , comprising contacting a plant element with a formulation comprising a designed complex endophyte , wherein the designed complex endophyte comprises a host fungal endophyte comprising a heterologous bacterium living inside the host fungal hyphae; as compared to an isoline plant not associated with the bacterium or the fungus.120. The method of claim 119 , wherein the plant element is a plant reproductive element and the method further comprises growing the plant from the plant reproductive element.121. The method of claim 119 , wherein said trait of agronomic importance is selected from the group consisting of: germination rate claim 119 , emergence rate claim 119 , shoot biomass claim 119 , seedling root length claim 119 , seedling shoot length claim 119 , seedling mass claim 119 , seedling root surface area claim 119 , and yield.122. The method of claim 119 , wherein the trait of agronomic importance is improved under normal watering conditions.123. The method of claim 119 , wherein the trait of agronomic importance is improved under conditions of water limitation.124. The method of claim 119 , wherein the designed complex endophyte is present in the formulation in an amount capable of modulating at least one of: a trait of agronomic importance claim 119 , the transcription of a gene claim 119 , the expression of a protein claim ...

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Номер записи: 89

24-12-2015 дата публикации

ENDOPHYTIC MICROBIAL SYMBIONTS IN PLANT PRENATAL CARE

Номер: US20150366217A1

Автор: Germida James J., Vujanovic Vladimir

Принадлежит:

The present disclosure provides novel endophyte strains or cultures thereof that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom. 1Streptomyces. An isolated endophyte of sp. strain or culture thereof which is deposited under IDAC 081111-06 or which comprises the 16S rDNA sequence as shown in SEQ ID NO:6.2Paraconyothirium. An isolated endophyte of sp. strain or culture thereof which is deposited as IDAC 081111-03 or which comprises the ITS rDNA sequence as shown in SEQ ID NO:5.3Pseudeurotium. An isolated endophyte of sp. or culture thereof which is deposited under IDAC 081111-02 or which comprises the ITS rDNA sequence as shown in SEQ ID NO:4.4Penicillium. An isolated endophyte of sp. or culture thereof which is deposited under IDAC 081111-01 or which comprises the ITS rDNA sequence as shown in SEQ ID NO:3.5Cladosporium. An isolated culture of sp. which is deposited under IDAC 200312-06 or which comprises the ITS rDNA sequence as shown in SEQ ID NO:1.6Cladosporium. An isolated endophyte of sp. or culture thereof which is deposited under IDAC 200312-05 or which comprises the ITS rDNA sequence as shown in SEQ ID NO:2.76. A composition comprising the isolated endophyte or culture of any one of - or a combination or mixture thereof and a carrier.86. A seed comprising the endophyte or culture of any one of - or a combination or mixture thereof.9. The seed of claim 8 , wherein the seed is of a cereal claim 8 , pulse claim 8 , flax or canola plant.106. A method of improving seed vitality claim 8 , plant health and/or yield comprising inoculating a seed with the endophyte or culture of any one of - or a combination or mixture thereof or with the composition of ; and cultivating the seed ...

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Номер записи: 90

20-12-2018 дата публикации

METHODS OF CULTIVATING ECTOMYCORRHIZAL FUNGI

Номер: US20180359982A1

Автор: GOLAN Ezekiel

Принадлежит:

A method of inoculating a tree with an ectomycorrhizal fungus is disclosed. The method comprises contacting at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of the at least one non-terrestrial adventitious root by the ectomycorrhizal fungus. Methods of cultivating the ectomycorrhizal fungus are also disclosed as well as systems and devices for same. 1. (canceled)2. A method of cultivating an ectomycorrhizal fungus on a tree , comprising growing the ectomycorrhizal fungus on at least one inoculated non-terrestrial adventitious root of the tree , thereby cultivating the ectomycorrhizal fungus on the tree.3. The method of claim 2 , further comprising contacting said at least one non-terrestrial adventitious root of the tree with the ectomycorrhizal fungus under conditions suitable for inoculation of said at least one adventitious root by the ectomycorrhizal fungus prior to said growing claim 2 , wherein said growing is effected in a container.4. (canceled)5. The method of claim 2 , wherein said tree is at least one meter high.67-. (canceled)8Tuber, Cantharellus, Boletus, Tricholoma, Hydnum, Lepista, Amanita, Russula, Morchella, Rhizopogon, Craterellus, Lactarius, Polyozellus, Chroogomphus, Laccaria, Albatrellus, Suillus, Amanita, Cortinarius, Gomphus, EntolomaInocybe.. The method of claim 2 , wherein said ectomycorrhizal fungus is an edible ectomycorrhizal fungus of a genus selected from the group consisting of and911-. (canceled)12. The method of claim 2 , wherein said tree is of the Fagales order.1314-. (canceled)15. The method of claim 3 , wherein said contacting is effected by:(a) planting said non-terrestrial adventitious root in soil adapted for growth of said ectomycorrhizal fungus which comprise an inoculum of said ectomycorrhizal fungus, or(b) planting said non-terrestrial adventitious root in soil adapted for growth of said ectomycorrhizal fungus and inoculating said soil with ...

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Номер записи: 91

26-11-2020 дата публикации

METHOD FOR INCREASING WHEAT YIELD AND PREVENTING WHEAT DISEASES AND USE THEREOF

Номер: US20200367507A1

Автор: Chen Tao, CHENG Jiasen, FU Yanping, JIANG Daohong, TIAN Binnian, XIE Jiatao

Принадлежит:

The present invention provides a method for increasing wheat yield and preventing wheat diseases and use thereof, and relates to the technical field of crop cultivation. In the method, wheat seeds are primed by a fermentation liquid of , and the concentration of the fermentation liquid is 1.4*10cfu/mL or more. According to the present invention, the wheat seeds are primed by using , and can endogenously grow in wheat plants to induce expression of genes related to disease resistance and development of wheat, thereby achieving the objective of controlling diseases such as wheat head blight and improving wheat yield. 1S. sclerotiorum. A method for increasing wheat yield and preventing wheat diseases , wherein wheat seeds are primed by a fermentation liquid of , and the concentration of the fermentation liquid is 1.4*10cfu/mL or more.2S. sclerotiorum. The method according to claim 1 , wherein the comprises hypovirulent strain DT-8 and/or virulent strain DT-8VF.3S. sclerotiorum. The method according to claim 1 , wherein a method for preparing the fermentation liquid of the comprises:{'i': S. sclerotiorum', 'S. sclerotiorum, 'sup': '5', 'inoculating the strain into a liquid medium, and fermenting at 18-22° C. to obtain a fermentation liquid of with the concentration of 1.4*10cfu/mL or more.'}4. The method according to claim 3 , wherein the fermentation time is 70-96 h.5. The method according to claim 3 , wherein the fermentation is accompanied by stirring with a stirring speed of 180-210 r/min.6. The method according to claim 1 , wherein the priming of the wheat seeds comprises the following steps:(1) sterilizing surfaces of wheat seeds to be treated and then soaking the wheat seeds in water to obtain water-absorbing wheat seeds after the wheat seeds absorb water and swell but do not begin to show white buds; and{'i': 'S. sclerotiorum', '(2) soaking the water-absorbing wheat seeds for 3-6 h with the fermentation liquid of the , and then drying to constant weight.'}7. The ...

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Номер записи: 92

26-11-2020 дата публикации

SALT-TOLERANT PLANTS

Номер: US20200370065A1

Автор: NIELSEN BRENT L.

Принадлежит:

A salt tolerant plant is created by inoculation of a glycophyte plant with bacteria isolated from a halophyte plant. 2AllenrolfeaSalicorniaSarcocornia.. The plant of wherein the halophyte plant is of genus claim 1 , or genus claim 1 , or genus3Allenrolfea occidentalis, Salicornia rubraSarcocornia utahensis.. The plant of claim 1 , wherein the halophyte plant is claim 1 , or4Halomonas, KushneriaBacillus.. The plant of claim 1 , wherein the bacteria is from genus claim 1 , or5. The plant of claim 1 , wherein the bacteria have at least one sequence recorded in GenBank under accession numbers MK873873 to MK873913.6. The plant of claim 1 , wherein the bacteria have one or more of the Illumina sequence reads available at the NCBI Sequence Archive under BioProject ID PRJNA553550 claim 1 , BioSample accessions SAMN12238110 claim 1 , SAMN12238111 claim 1 , SAMN12238112 claim 1 , SAMN12238113 claim 1 , SAMN12238114 claim 1 , SAMN12238115 claim 1 , SAMN12238116 claim 1 , SAMN12238117 claim 1 , SAMN12238118 claim 1 , SAMN12238119.7. The plant of claim 1 , wherein the glycophyte plant is from alfalfa claim 1 , Kentucky blue grass claim 1 , or Bermuda grass.8. The plant of claim 1 , wherein the glycophyte plant is from a grass.9. The plant of claim 8 , wherein the grass is from a turf grass.10. The plant of claim 9 , wherein the turf grass is from Kentucky blue grass claim 9 , or Bermuda grass.11. A method for creating an artificial salt tolerant plant comprising:inoculating a glycophyte plant with a non-host halophile bacteria into the plant rhizosphere or as an endophyte to form the salt tolerant plant, the salt tolerant plan having a symbiotic relationship between the plant and the non-host halophile bacteria to provide growth promotion to the salt tolerant plant under saline conditions, the symbiotic relationship being an artificial plant/bacteria combination that does not otherwise naturally occur, the non-host halophile bacteria being identifiable as a naturally occurring ...

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Номер записи: 93

10-12-2020 дата публикации

Large-Scale Aerobic Submerged Production of Fungi

Номер: US20200385669A1

Автор: ALIBEK Ken, DIXON Tyler, FARMER Sean, MAZUMDER Sharmistha

Принадлежит:

Methods are provided for cultivating fungi for scaled-up production of microbe-based products. Specifically, cultures of fungi, such as, e.g., , are grown by aerobic submerged fermentation in liquid medium containing a particulate anchoring carrier to increase mycelial biomass. 2. The method of claim 1 , used to produce and accumulate fungal mycelia.3. The method of claim 1 , wherein the anchoring carrier comprises a sterilized material comprising a plurality of individual pieces with diameter of about 0.5 mm to about 3 mm.4. The method of claim 3 , wherein the individual pieces are whole claim 3 , or pieces of claim 3 , seeds claim 3 , nuts claim 3 , beans claim 3 , or fruits.5. The method of claim 3 , wherein the anchoring carrier comprises alginate beads.6. The method of claim 5 , wherein the alginate beads are prepared by dropping a solution comprising 3% sodium alginate solution claim 5 , and claim 5 , optionally claim 5 , nutrients and/or fungal inoculant claim 5 , into a sterile 5% calcium chloride solution.7. The method of claim 1 , wherein the fungal-carrier mass is harvested from the reactor and claim 1 , dried to produce a dry fungi-based product.8. The method of claim 7 , wherein the dry fungi-based product is milled.9. The method of claim 7 , wherein the fungal strain is separated from the carrier prior to drying.1011-. (canceled)1211. The method of claim claim 7 , wherein the components of the liquid growth medium are sterilized prior to inoculation.13. (canceled)14. The method of claim 12 , wherein sterilization comprises mixing the components with 3% hydrogen peroxide in a ratio of 1:3 (w/v).15. The method of claim 1 , wherein the fungal strain is a strain of ectomycorrhizal fungi.16Pisolithus tinctorius.. The method of claim 15 , wherein the fungal strain is a strain of17. The method of claim 1 , wherein the one or more antibacterial substances include streptomycin and/or oxytetracycline.18. The method of claim 1 , wherein the one or more ...

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Номер записи: 94

17-12-2020 дата публикации

Endophyte Screening

Номер: US20200390055A1

Автор: JOHNSON Richard David, SIMPSON Wayne Roydon, Tsujimoto Hisashi

Принадлежит:

The present invention relates to isolated strains of endophytes that form stable symbiotic associations with cereal grasses, particularly wheat, wherein the symbiotic associations are combinations of endophytes and host plants that are not found in nature The invention also relates to methods of identifying and/or selecting fungal endophytes that form stable symbiotic associations with wheat plants including methods of screening for fungal endophytes that form stable symbiotic associations with wheat plants, and to methods of screening for wheat plants that form stable symbiotic associations with fungal endophytes. 119.-. (canceled)20. A method of identifying a wheat plant that forms a stable symbiotic combination with a fungal endophyte comprisingartificially inoculating a fungal endophyte into a candidate wheat plant to form a wheat plant/endophyte combination,propagating the wheat plant/endophyte combination,obtaining seed from the propagated combination, andidentifying the presence of the endophyte in the seed, andoptionally selecting a wheat plant that is capable of forming a stable symbiotic combination with a fungal endophyte.21. A method of making a stable symbiotic combination comprising a wheat plant and a fungal endophyte comprisingartificially inoculating a fungal endophyte into a candidate wheat plant to form a wheat plant/endophyte combination,propagating the wheat plant/endophyte combination,obtaining seed from the propagated combination, andidentifying the presence of the endophyte in the seed, andoptionally selecting a stable symbiotic combination comprising the wheat plant and the fungal endophyte.22Elymus. The method of claim 21 , wherein the fungal endophyte is isolated from spp.23Epichloë. The method of claim 21 , wherein the fungal endophyte is an fungal endophyte.24EpichloëElymusHordeum. The method of claim 21 , wherein the fungal endophyte is an fungal endophyte isolated from wild cereal grasses selected from the group consisting of spp. ...

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Номер записи: 95

17-12-2020 дата публикации

Materials and Methods for Control of Insect Pests Using Entomopathogenic Fungi

Номер: US20200390106A1

Автор: ALIBEK Ken, FARMER Sean

Принадлежит:

The subject invention relates to the production and use of entomopathogenic fungi for the control of arthropod pests. Specifically, the subject invention provides methods for producing and as well as methods of their use, as biopesticidal compositions. Compositions are also provided for controlling arthropod pests, comprising one or more entomopathogenic fungi, a chitinase inducer, diatomaceous earth, and an anticoagulant.

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Номер записи: 96

17-11-2022 дата публикации

ENDOPHYTIC FUNGUS PSEUDOPHIALOPHORA ORYZAE P-B313 AND ITS APPLICATION

Номер: US20220361506A1

Автор: Li Lin, LIANG YAN, LIN FUCHENG, SHEN KUNLUN, SU ZHENZHU

Принадлежит:

The present invention discloses an endophytic fungal strain sp. P-B313 and an application thereof, and belongs to the technical filed of microbial applications. The deposit number of the endophytic fungal strain P-B313 is CCTCC M 2021504, and the scientific name thereof is sp. The endophytic fungal strain P-B313 can enhance the resistance of rice against seedling leaf blast with a control efficiency of 87.56% and a disease index reduced by 62.59. The biological control efficiency of the endophytic fungus P-B313 against seedling leaf blast in rice has great value by promotion and applications thereof in the field of agriculture. 1PseudophialophoraPseudophialophora. An endophytic fungus sp. P-B313 , wherein the deposit number thereof is CCTCC M 2021504 and the scientific name thereof is sp.2Pseudophialophora. The endophytic fungus sp. P-B313 of claim 1 , wherein the ITS sequence of the strain is as set forth in SEQ ID No. 1.3Pseudophialophora. The endophytic fungus sp. P-B313 of claim 1 , wherein the colony diameter reaches 6 cm after the strain grows on a PDA medium plate at 25° C. for 10 days; aerial mycelia are poorly developed claim 1 , prostrating on a surface of the medium claim 1 , and the colony is white;the mycelia are 0.5-4.0 μm in width, with a septum; conidiophores are solitary, unbranched;conidia are elliptic or dumbbell-shaped, 11-15 μm×3.5-6.5 μin size, with protrusions at the apex thereof and with a septum, the number of the septum being 0-1.4Pseudophialophora. A method of controlling of rice blast comprising the step of utilizing the endophytic fungus sp. P-B313 of .5Pseudophialophora. The method of claim 4 , comprising: colonization of the endophytic fungus sp. P-B313 in a root tissue of rice.6Pseudophialophora. The method of claim 5 , comprising: co-cultivation of germinated rice seeds and the endophytic fungus sp. P-B313 for colonization of the fungus in roots of rice seedlings to enhance resistance of rice against seedling leaf blast at seedling ...

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Номер записи: 97

23-04-2019 дата публикации

一种蓟马防治剂及其应用

Номер: CN106135295B

Автор: 张泽华, 涂雄兵, 赵亚斋

Принадлежит: Institute of Plant Protection of Chinese Academy of Agricultural Sciences

本发明公开了一种蓟马防治剂及其应用。本发明所提供的蓟马防治剂，为蓟马防治剂甲或蓟马防治剂乙；所述蓟马防治剂甲包括白僵菌、增效剂和滑石粉；所述蓟马防治剂乙包括所述白僵菌、氯虫苯甲酰胺和所述滑石粉；所述白僵菌为球孢白僵菌(Beauveria bassiana)BBNS‑J9‑16 CGMCC No.5288。实验证明，将本发明提供的蓟马防治剂和沙子混合后，撒施土壤进行防治，第14天后，防治效果达到75％；将本发明提供的蓟马防治剂喷施苜蓿，第14天后，防治效果达到70％。因此，本发明提供的蓟马防治剂可有效的防治蓟马，具有重要的应用价值。

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Номер записи: 98

28-06-2011 дата публикации

Formulation for controlling plant diseases containing bikaverin and method for controlling plant diseases using same

Номер: KR101044273B1

Автор: 김진철, 임희경, 장경수, 조광연, 최경자

Принадлежит: 한국화학연구원

본 발명은 항균물질인 비카베린을 생산하는 비병원성 후자리움 옥시스포룸 ( Fusarium oxysporum ) EF119 균주, 비카베린 또는 EF119 균주를 포함하는 미생물 제제 및 상기 미생물 제제를 이용하여 식물병을 방제하는 방법에 관한 것이다. 본 발명의 후자리움 옥시스포룸 EF119 균주 및 후자리움 옥시스포룸 EF119 균주가 생산하는 비카베린은 시험관내( in vitro ) 및 생체내( in vivo )에서 다양한 식물 병원균의 생장을 억제하므로, 균주의 배양액, 균사체 또는 포자를 이용하거나 비카베린을 이용하여 식물병을 환경친화적으로 방제함으로써 고부가가치의 유기농산물을 생산할 수 있다. The present invention relates to a microbial agent comprising a non-pathogenic Fusarium oxysporum EF119 strain, a non-caberine or an EF119 strain, which produces the anti-cababerine, and a method of controlling plant diseases using the microbial agent. . The vicarinine produced by the Fusium oxysporum EF119 strain and the Fussium oxysporum EF119 strain of the present invention is in vitro ( in Inhibit the growth of various plant pathogens in vitro and in vivo , and can produce high value-added organic products by environmentally friendly control of plant diseases using culture medium, mycelium or spores of the strain or by using vicarinine. have.

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Номер записи: 99

02-01-1992 дата публикации

A method and device for the biological control of insects

Номер: EP0462978A1

Автор: Carol Ann Johnson, Fernando Agudelo-Silva, Haim B. Gunner, Meir Broza

Принадлежит: EcoScience Corp

Un procédé et un dispositif insecticides commodes, économiques, non toxiques et efficaces permettent d'éliminer des insectes en les attirant vers une concentration élevée d'un champignon entomopathogène, par exemple, Metarhizium anisopliae et Beauveria bassiana, de sorte qu'ils soient infectés par le champignon et meurent. Les champignons sont placés dans l'environnement que l'on veut traiter au moyen d'un dispositif qui assure le contact des insectes avec les champignons et leur infection. Dans un mode préférentiel d'élimination biologique de cafards, les cafards sont exposés aux champignons au moyen d'une chambre de contamination pourvue d'ouvertures à travers lesquelles les cafards pénètrent et entrent en contact avec une culture de champignons vivants pathogènes pour les cafards. Les spores des champignons s'attachent aux cafards, germinent et pénètrent dans leur corps, provoquant la mort des cafards infectés. La mort survient de deux à trois semaines approximativement après le contact avec la culture. Pendant ce temps, les cafards infectés distribuent les spores du champignons pathogène dans les zones infectées, ces spores pouvant infecter ultérieurement d'autres cafards. Dans les conditions ambiantes appropriées, les champignons produisent des spores sur le cadavre des cafards infectés et les conidies peuvent être transmises à d'autres cafards, propageant la maladie. A convenient, economical, nontoxic and effective insecticidal method and device eliminates insects by attracting them to a high concentration of an entomopathogenic fungus, for example, Metarhizium anisopliae and Beauveria bassiana, so that they are infected with the fungus and die. The fungi are placed in the environment to be treated by means of a device which ensures the contact of insects with the fungi and their infection. In a preferred method of biological elimination of cockroaches, cockroaches are exposed to fungi by means of a contamination ...

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Номер записи: 100