11-07-2013 дата публикации
Номер: US20130177539A1
Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is , or . Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided. 1. An isolated polynucleotide comprising a nucleotide sequence comprising:(a) the nucleotide sequence comprising any one of nucleotides 1-380 of SEQ ID NO: 45; SEQ ID NO: 45; SEQ ID NO: 13; SEQ ID NO: 15; nucleotides 1-266 of SEQ ID NO:50; SEQ ID NO:50; SEQ ID NO: 18; SEQ ID NO: 29; nucleotides 1-675 of SEQ ID NO:37, SEQ ID NO: 37; SEQ ID NO:16; SEQ ID NO:32; SEQ ID NO:25; nucleotides 1-132 of SEQ ID NO: 40; or any one of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 17, 19, 20, 21, 22, 23, 24, 26, 27, 28, 30, 31, 33, 34, 35, 36, 38, 39, 41, 42, 43, 44, 46, 47, 48, 49, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, ...
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