17-02-2022 дата публикации
Номер: US20220049193A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions. 1. A lipase variant of a parent lipase , wherein the parent lipase is SEQ ID NO: 1 , wherein said variant:(a) comprises a modification in at least one position corresponding to positions E1, V2, N33, F51, E56, L69, K98, V176, H198, E210, Y220, L227, and K237 of SEQ ID NO: 1; and optionally further comprises a modification in at least one position corresponding to positions D27, G38, D96, D111, G163, T231, N233, D254, and P256 of SEQ ID NO: 1;(b) has a sequence identity of at least 75% but less than 100% to SEQ ID NO: 1; and(c) has lipase activity.2. The lipase variant according to claim 1 , comprising modification in at least one of the following positions: E1 claim 1 , V2 claim 1 , D27 claim 1 , N33 claim 1 , G38 claim 1 , F51 claim 1 , E56 claim 1 , L69 claim 1 , D96 claim 1 , K98 claim 1 , D111 claim 1 , G163 claim 1 , V176 claim 1 , H198 claim 1 , E210 claim 1 , Y220 claim 1 , L227 claim 1 , T231 claim 1 , N233 claim 1 , K237 claim 1 , D254 claim 1 , and P256 of SEQ ID NO: 1.3. The lipase variant according to claim 1 , comprising at least one of the following substitutions: E1C claim 1 , V2Y claim 1 , D27R claim 1 , N33K claim 1 , N33Q claim 1 , G38A claim 1 , F51V claim 1 , E56K claim 1 , L69R claim 1 , D96E claim 1 , D96L claim 1 , K98I claim 1 , K98Q claim 1 , D111A claim 1 , G163K claim 1 , V176L claim 1 , H198S claim 1 , E210K claim 1 , Y220F claim 1 , L227G claim 1 , T231R claim 1 , N233R claim 1 , N233C claim 1 , K237C claim 1 , D254S claim 1 , and P256T of SEQ ID NO: 1.4. The lipase variant according to comprising one of the following substitutions: S54T claim 1 , S83T claim 1 , G91A claim 1 , A150G claim 1 , I255A claim 1 , and E239C of SEQ ID NO: 1.56-. (canceled)7. The lipase variant according to claim 1 , ...
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