16-05-2013 дата публикации
Номер: US20130122571A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase. 1. A TY145 like subtilase variant which is at least 63% homologous to the sequence of SEQ ID NO:1 , which variant comprises: a) the Weak ion-binding site are: 154, 155, 158, 164, 165, 166, 167, 168, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 211, 220, 221, 222, 223, 224, 225, 226, 227, 228, 277, 281 and 305,', 'b) the Near ion-binding site are: 185, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 277, 281, 299, 300, 301, 304, 305,', 'c) the Far ion-binding site are: 193, 198, 199, 201, 202, 204, 216, 217, 219, 226, 227, 228, 229, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306 and 307;, '(1) an alteration in one or more positions located at a distance of not more than 10 Å to one of the ion-binding sites of TY145, wherein the positions, as specified in SEQ ID NO:1, located at a distance of not more than 10 Å to 84, 85, 86, 87 and 88,', '108, 109, 110, 111, 112, 113, 114, 115, 116 and 117,', '141, 142, 143, 144, 145 and 146,', '150, 151 and 152,', '169, 170 and 171,', '200 and 201,', '211, 212, 213, 214, 215, 216, 217, 218, 219 and 220,', '242 and 243, 268, 269 and 270;, '(2) one or more alterations in one or more of the positions contained in the following highly mobile regions 1, 2, 3, 4, 5, 6 and 7,', '17, 18, 19, 20, 21, 22 and 23,', '38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 and 50,', '57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68 and 69,', '84, 85, 86, 87, 88, 89, 90, 91 and 92,', '107, 108, 109 and 110,', '239, 240, 241, 242 and 243', '265 and 266,', 'wherein said alterations preferably are introduced in one or both of the regions 57-69 and 84-92;, '(3) one or more alterations in one or more of the ...
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