УСТРОЙСТВО ДЛЯ КУЛЬТИВИРОВАНИЯ КЛЕТОЧНЫХ КУЛЬТУР

Изобретение относится к области культивирования клеток и тканей. Предложено устройство для культивирования клеточных культур. Устройство включает наружный трубчатый корпус с верхним концом и нижним концом. Верхний конец имеет отверстие, а нижний конец закрыт мембраной с внутренней поверхностью и наружной поверхностью для высевания биологического материала. Устройство содержит дополняющий трубчатый корпус с внутренним диаметром, соответствующим наружному диаметру нижнего конца наружного трубчатого корпуса, а также подвесной элемент с верхним концом и нижним концом. От верхнего конца подвесного элемента в сторону отходит фланец, а наружный диаметр подвесного элемента соответствует внутреннему диаметру наружного трубчатого корпуса. Наружный трубчатый корпус на первом этапе культивирования соединен с дополняющим трубчатым корпусом с образованием стоящей вставки. На втором этапе наружный трубчатый корпус выведен из зацепления с дополняющим трубчатым корпусом и в перевернутом положении прикреплен ...

УСОВЕРШЕНСТВОВАННЫЙ СПОСОБ ПОЛУЧЕНИЯ МАКРОГРАНУЛ

Изобретение относится к области биохимии. Предложен способ получения композиции вещества, содержащей образец живых клеток в грануле. Способ включает смешивание первого образца агарозы с живыми клетками с образованием суспензии, перенос суспензии в первый образец минерального масла для образования гранулы, извлечение гранулы из первого образца, удаление минерального масла с гранулы, перенос гранулы во второй раствор агарозы, покрытие гранулы вторым раствором агарозы, извлечение гранулы из второго раствора и дозирование гранулы с покрытием во второй образец минерального масла. При этом образец минерального масла поддерживают при температурном градиенте, так что указанная гранула перемещается в минеральном масле по направлению от более высокой температуры, от 20 до 30°C, к более низкой температуре, от 0 до -8°C. Изобретение обеспечивает получение гранул с более однородной формой, а также автоматизацию процесса. 9 з.п. ф-лы., 7 ил., 8 пр.

УСТРОЙСТВО ДЛЯ КУЛЬТИВИРОВАНИЯ ОРГАНИЗМОВ И СПОСОБ КУЛЬТИВИРОВАНИЯ ОРГАНИЗМОВ

Изобретение относится к биотехнологии. Устройство содержит сосуд для культуральной среды и расположенный в нем контактирующий с ней микропористый носитель, служащий для размещения на нем выращиваемой культуры и подвода к ней через поры культуральной среды. Микропористый носитель изготовлен формованием обожженного неметаллического неорганического твердого материала. Культивируемые организмы представляют собой клетки ткани растений, грибов или бактерий. Согласно способу, осуществляемому в предложенном устройстве. клетки или ткани помещают на верхнюю поверхность микропористого носителя. Нижнюю часть носителя погружают в культуральную среду, которая поступает к верхней поверхности микропористого носителя, для обеспечения ее поглощения выращиваемой культурой. Изобретение обеспечивает упрощение обслуживания устройства, уменьшение расхода культуральной среды, расширение области применения устройства. 2 н. и 5 з.п. ф-лы, 30 ил., 6 табл.

ФЛАКОН ДЛЯ ПРИГОТОВЛЕНИЯ ЦИТОЛОГИЧЕСКОЙ СУСПЕНЗИИ

... 1. Флакон для приготовления цитологической суспензии на основе фиксатора, оборудованный средствами (4) фильтрования, по меньшей мере, частично погруженными в суспензию, отличающийся тем, что средства (4) фильтрования выполнены в виде сетки из фильтрующего материала, образующие корзинку, периферию (5), которая крепится на флаконе, центр (6) которой соединен с трубкой (7), выполненной в направлении отверстия (2) флакона и соединенной со средствами (8) удержания в флаконе, и которая выполнена с возможностью прохождения через нее пипетки для всасывания суспензии. 2. Флакон по п.1, отличающийся тем, что верхняя часть (9) флакона выполнена с возможностью установки на ней крышки (10). 3. Флакон по п.2, отличающийся тем, что крышка (10) и соответствующая часть (9) флакона (1) содержат дополняющие друг друга средства завинчивания. 4. Флакон по одному из пп.2 или 3, отличающийся тем, что напротив соответствующего конца трубки (7) крышка (10) содержит, по меньшей мере, один прокалываемый и самозатягивающийся ...

Аппарат для культивирования микроорганизмов

Дрожжерастильный аппарат

Verbesserter Träger für biologische Züchtung und Verfahren zur Herstellung

Disposable bioreactor for the cultivation of cells in a nutrient medium, comprises a reagent container, which is lockable with a lid, exhibits a baffle adjacent to the container inner wall and is subjected to a vibrating device

The disposable bioreactor (1) for the cultivation of cells in a nutrient medium, comprises a reagent container (2), which is lockable with a lid (3), exhibit baffles adjacent to the container inner wall (7) and is subjected to a vibrating device. The baffles are formed as a helically shaped channel (10) running upwards in vertical direction. The channel is shaped as a convex projection to the container inner wall and is rounded off at the intersection to the container inner wall. The channel has a gradient angle of 30-60[deg] with respect to a horizontal plane. The disposable bioreactor (1) for the cultivation of cells in a nutrient medium, comprises a reagent container (2), which is lockable with a lid (3), exhibit baffles adjacent to the container inner wall (7) and is subjected to a vibrating device. The baffles are formed as a helically shaped channel (10) running upwards in vertical direction. The channel is shaped as a convex projection to the container inner wall and is rounded off ...

VORRICHTUNG ZUM AUFNEHMEN UND TRANSPORTIEREN EINER BAKTERIEN-KULTUR

KULTIVIERUNGSGEFAESS FUER BLUT MIT EINEM EXTERN BETRIEBENEN VENTIL.

KULTIVATIONSGEFAESS FUER MIKROORGANISMEN, ZELLEN UND GEWEBE

SICHTPLATTE FUER MIKROTESTPLATTEN

Probenbehälter zur Aufbewahrung und Verarbeitung von mit einem Probenentnahmewerkzeug entnommenen Proben

Ein Probenbehälter (10) zur Aufbewahrung und Verarbeitung von mit einem Probeentnahmewerkzeug (50) entnommenen Proben, vorzugsweise Proben umfassend biologisches Material, umfasst einen ersten Behälter (12) und einen den ersten umgebenden zweiten Behälter (14), wobei der erste (12) und der zweite Behälter (14) relativ zueinander in eine Aufbewahrungsstellung bringbar sind, in welcher ein zwischen erstem (12) und zweitem Behälter (14) gebildeter Sammelraum (16) ein erstes, kleineres Volumen aufweist, und in eine von der Aufbewahrungsstellung verschiedene Analysestellung bringbar sind, in welcher der Sammelraum (16) ein vom ersten verschiedenes zweites, größeres Volumen aufweist, wobei am ersten Behälter (12) ein Festlegemittel vorgesehen ist, welches wenigstens in der Analysestellung mit einem am zweiten Behälter (14) vorgesehenen Festlegegegenmittel derart in Festlegeeingriff bringbar ist, dass eine Relativbewegung der beiden Behälter (12, 14) in wenigstens einer Relativbewegungsrichtung ...

Behälter mit flexibler Wandung

Die Erfindung umfasst einen Behälter (1) mit flexibler Wandung (2) und mindestens einem durch einen Wandungsdurchbruch (7) in den von der flexiblen Wandung (2) umgebenen Behälterinnenraum (3) hineinragenden Entleerungsrohr (4, 4´, 4´´), dessen freies Ende (12) eine Entleerungsöffnung (13) aufweist, die in ihrer Lage in vertikaler Richtung auf unterschiedliche Entleerungshöhen (14) einstellbar ist. Der Behälter (1) zeichnet sich dadurch aus, dass in dem Wandungsdurchbruch ein Adapterstück (8) angeordnet ist, in dem das Entleerungsrohr (4, 4´, 4´´) längsverschieblich gehaltert ist und dass das Entleerungsrohr (4, 4´, 4´´) in einem Teilbereich von einer flexiblen Umhüllung (5) umgeben ist, die an einem ersten Ende (11) mit dem Entleerungsrohr (4, 4´, 4´´) und an ihrem dem ersten Ende (11) abgewandten zweiten Ende (10) mit der Wandung (2) oder dem Adapterstück (8) verbunden ist.

Membranreaktorsystem

Die vorliegende Erfindung betrifft eine Vorrichtung mit einem Membranreaktor, worin zwei Kompartimente durch eine Membran getrennt sind. Die Erfindung stellt eine Verbesserung beim Betrieb solcher Membranreaktoren dar.

Behälterverschluss mit integrierter Sicherungseinrichtung für einen Probenträger

Behälterverschluss mit integrierter Sicherungseinrichtung für einen Probenträger, wobei der Behälterverschluss (3) die Form eines Schraub- oder Drehverschlusses, eines Deckels, einer Kappe, eines Stopfen oder dgl. aufweist und der Probenträger ein Tupfer mit einer an einem Tupferstiel (2) angebrachten Probenaufnahme (1) in Form eines Wattebauschs (1) oder einer anderen geeigneten Aufnahme ist, dadurch gekennzeichnet, dass das der Behälterverschluss (3) mit Mitteln zur Arretierung des Tupferstiels (2) im Führungsrohrs (4) ausgestattet ist, wobei diese ein einmaliges Einschieben des Tupferstiels (2) in das Führungsrohr (4) zulassen und eine Bewegung in entgegengesetzter Richtung verhindern.

Bioreaktor

Bioreaktor mit mindestens einer Filtertasche, die an der Innenfläche einer Wand des Bioreaktors (3) fixiert ist und in ihrem Inneren über einen Sammel- und/oder Verteilerraum (6) verfügt, der mit mindestens einem Anschluss (7) für ein Zu- und/oder Abführen von Medien kommunizierend verbunden ist, wobei wenigstens eine Filtertaschenwand zumindest teilweise durch ein hydrophiles Filtermedium (4) gebildet ist, dadurch gekennzeichnet, dass das Filtermedium in Wasser einen Quellungs- und/oder Schrumpfungsgrad von höchstens 1% aufweist.

Fermenter zur aneroben Vermehrung von Effektiven Mikroorganismen

Fermenter/Brüter zur Vermehrung von Effektiven Mikroorganismen, dadurch gekennzeichnet, das Kunststoffkanister in den Größen 3, 5 und 10 Liter verwendet werden.

Zuchtreaktor für landwirtschaftliche und sonstige hochreine Minikulturen mit automatischer Dünge-und Erntetechnik

Der Zuchtreaktor für landwirtschaftliche und sonstige hochreine Minikulturen mit automatischer Dünge- und Erntetechnik ist dadurch gekennzeichnet, dass das Kunststoffschlauch-Modul aus speziellem mehrwandigem Material besteht, welches optimalen Lichtdurchlass für die Minikulturen gewährleistet, sowie eine dichte stoffliche Verbindung mit den Versorgungsschlauch-Anschluss-Pats gem. Punkt 2 gewährleistet.

Rührer für Bioreaktor

Rührer (1, 1', 1'', 1''', 1'''') für Bioreaktoren, die insbesondere als Behälter (21) mit flexiblen Wänden (22) ausgebildet sind, mit einer Mehrzahl gelenkig jeweils um eine Querachse (11) verschwenkbarer miteinander verbundener Arme (2, 3, 28, 29), die gemeinsam um eine Drehachse (10) drehbar sind, wobei ein erster Arm (2) mit seinem ersten Armende (9) an einem ersten um die Drehachse (10) drehbaren Achsteil (7) einer ersten Halterung (4) angeordnet ist, die an einer ersten Seite (23) des Behälters (21) befestigt ist, dadurch gekennzeichnet, dass der dem ersten Arm (2) abgewandte letztfolgende Arm (3, 28, 29) mit seinem dem ersten Arm (2) abgewandten zweiten Armende (18, 20, 30) an einem um die Drehachse (10) drehbaren Achsteil (16, 16''', 16'''') einer zweiten Halterung (5, 5''', 5'''') angeordnet ist, dass die zweite Halterung (5, 5''', 5'''') an einer der ersten Seite (23) des Behälters (21) gegenüberliegenden zweiten Seite (24) des...

APPARATUS FOR DIFFERNTIATING BACILLI

... 1366950 Microbiological testing DIAGNOSTIC RESEARCH Inc 23 Dec 1971 60026/71 Heading C6F A system for differentiating enteric bacilli comprises three separate media, such that a surface of a second medium is completely covered by a surface of a first medium, the third medium being independent of the other two, and the first medium comprises peptone, yeast extract, phenylalanine, L-tryptophan, dextrose, lactose, sodium chloride, ferric ammonium citrate, sodium thiosulphate, agar, bromcresol purple and water, the second medium comprises peptone, yeast extract, lysine, dextrose, agar, bromcresol purple and water, and the third medium comprises peptone, yeast extract, L-ornithine, L-tryptophan, dextrose, agar, bromcresol purple and water. The second medium is semisolid whereby to detect the mobility of the bacteria under test. The third medium may also be made in a double pour with the ornithine-deficient medium on top and the tryptophan-deficient medium on the bottom. The media may be contained ...

Contamination-proof stopper,in particular screw cap,for cell culture bottles

A contamination-proof stopper, in particular a plastic screw cap for sterilised cell culture bottles (F) for cultivating and storing tissue cultures used for biological, virological, immunological and cytological analyses and diagnoses. The part (2) of the plastic stopper (1) that covers the bottle opening (F') incorporates a hydrophobic filtering element (4) made of a filtering material innocuous for the cell cultures (Z) and having a pore size or cut-off that allows a sterile gaseous exchange between the bottle content and the ambient atmosphere to take place and prevents any cellular or microbiological contamination from inside to the outside or vice-versa.

Fermentation flask for cultivating microorganisms, a growth media and a method of use

Diagnostic swabs

... A testing assembly for testing for the presence of micro-organisms comprises a sterile container (not shown) and a rigid applicator 16, provided with a solid mass of culture medium 18 at one end, removably housed in the container. The applicator may be made of wood or plastics material and may have one or more projections 17 to assist in the retention of the culture medium. The projection may be in the form of an arrowhead. The culture medium, which is supported in a cotton swab, should contain at least 3% by weight of agar. Formulations of culture media suitable for the identification of Candida albicans, Streptococci, Staphylococci, Proteus, and of general bacterial growth in urine are specified. The culture medium for determining the presence of Candida albicans includes a colour indicator (bismuth sulphite) and an antibiotic (neomycin) to inhibit bacterial growth while permitting any growth of Candida albicans and other moulds; these latter, including penicillium ...

Bulk cell culture vessel

A bulk cell culture vessel (10) comprising a generally cylindrical body part (12) and a separable end part (14) having, distinct from any closure cap provision for normal in-use access to the vessel, spaced peripheral formations (20, 22). One (20) enters the body part (12) and another (22) goes over the body part (12) with a seal (38) accommodation between those formations (20, 22). Said other peripheral formation (22) cooperates with exterior formation (32) of the body part (12) for the purposes of mutual securement and compression of a resilient seal (38) in said seal accommodation by an edge of the body part (12) going between the one (20) and the other (22) peripheral formations.

Apparatus and method for preparing and culturing cells

A cell culture vessel

Bacteriological MPN strip

This invention is a convenient device for the enumeration of micro- organisms in liquids or suspensions. It consists of a plastic screw capped container having septa, for the division of the sample into appropriate volumes, and Durham tubes moulded in. It contains a sterile growth medium in lyophilised, powder or tablet form. The container is aseptically filled with sample to a line marked on its side, agitated to dissolve the medium and layed flat on a plane surface. It is then rotated through 90 DEG about its long axis so that the desired volumes of sample are separated into their respective compartments. The Durham tubes are designed so that air escapes from them during this operation. Incubation may begin immediately using a portable incubator if necessary. The device was conceived with potable water bacteriology in mind but many other possible applications exist.

PREPARED NUTRIENT SUBSTRATE CARRIER

PISTON FOR THE PRODUCTION OF A ZYTOLOGI SUSPENSION

CONTAINER FOR THE CULTIVATION OF FABRICS

PREFABRICATED FERTILE SOIL CARRIER FOR THE QUALITATIVE AND QUANTITATIVE REGULATION OF MICROORGANISMS

VERFAHREN UND VORRICHTUNG ZUR PRUEFUNG VON EXKREMENTEN AUF ANWESENHEIT VON PARASITENEIERN

COMPOSITION AND PROCEDURE FOR THE DIAGNOSIS OF TUMORS

PROCEDURE AND DEVICE FOR THE PREPARATION OF A BIOLOGICAL URPROBE FOR THE REGULATION AT LEAST A IN IT CONTAINED COMPONENT

VORRICHTUNG ZUM NACHWEIS VON MIKROORGANISMEN

VORRICHTUNG ZUM ZUECHTEN EINER MIKROORGANISMEN- KULTUR

VORRICHTUNG ZUM NACHWEIS VON MIKROORGANISMEN

VORRICHTUNG ZUR UNTERSUCHUNG VON BAKTERIAEMIEN

VORGEFERTIGTER NAEHRBODENTRAEGER ZUR QUALITATIVEN UND QUANTITATIVEN BESTIMMUNG VON MIKROORGANISMEN

BREEDING MECHANISM FOR CO2-ABHAENGIGE MICROORGANISMS

PROCEDURE AND DEVICE FOR THE EXAMINATION OF EXKREMENTEN ON PRESENCE OF PARASITE EGGS

MICRO-ORGANISM GROWTH

VETERINARY TEST SET FOR RAPID TEST FOR THE DETERMINATION OF THE BLOOD CALCIUM CONTENT.

DEVICE TO THE PROOF OF MICROORGANISMS.

DEVICE AND PROCEDURE FOR IMMUNOASSAY.

CELL CULTURAL EMPLOYMENT.

ROLL BOTTLE WITH INCREASED SURFACE.

DEVICE FOR COLLECTING AND/OR PROTECTING BIOLOGICAL ZUECHTUNGEN ZUECHTEN.

DEVICE TO THE PROOF OF MICROORGANISMS.

CELL CULTURAL PISTON WITH A DIAPHRAGM BARRIER.

CELL CULTURAL CONTAINER FOR CULTIVATION NOT ADHÄRENTER CELLS

CARRIER DEVICE FOR TEST TUBES

Selection and cloning of T lymphocytes in a microfluidic device

Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.

Diagnostic test system and method

A diagnostic test system, including: a diagnostic test assembly and a diagnostic test apparatus to perform a test on a biological or environmental sample; the diagnostic test assembly includes: a sample preparation reservoir to receive the sample into a sample preparation fluid, such that a swab carrying the sample can be used to stir the preparation fluid and to wash the swab; a sample dispensing mechanism for insertion into the sample preparation reservoir; a closure to seal the sample preparation reservoir; at least one diagnostic test reservoir coupled to the sample preparation reservoir; and at least one seal between the sample preparation reservoir and the diagnostic test reservoir to prevent fluid movement between the respective reservoirs; wherein the sample dispensing mechanism is operable to disrupt the seal to allow sample fluid to enter the diagnostic test reservoir from the sample preparation reservoir, and to dispense a predetermined amount of fluid.

MICROBIAL PATHOGEN DETECTING SYSTEM

A container and method for storing a sample

A container, comprising: a body; and, a closure; wherein the closure has a lid hinged to a ring mounted on the body, the lid moveable from an open position to a closed position; wherein the lid has a tab hinged thereto for: inserting into and engaging a slot formed in the ring as the lid is moved to the closed position from the open position to thereby lock the lid in the closed position; or, receiving and engaging a pin head of a pin mounted on the ring in a hole formed in the tab to thereby hold the lid in the closed position, the pin configured to detach from the ring when the tab and lid are returned to the opened position.

Continuous Culture Apparatus with Mobile Vessel, Allowing Selection of Filter Cell Variants

Reaction casing for a photosynthetic reactor and associated photosynthetic reactor

The invention relates to a reaction casing (1) for a photosynthetic reactor (2), designed on the one hand to float on an expanse of water and, on the other hand, to define a path for a gas / liquid culture medium to flow in two phases between first (11) and second (12) openings of the casing (1). The casing (1) comprises respectively outer (3) and inner (4) claddings, at least partially made of a material that is transparent to visible radiation, the inner cladding (4) extending on the inside of the outer cladding (3) such that said claddings mutually define an inter-cladding space (10) in fluid connection with the first opening of the casing. The outer cladding has an open proximal end (30) and a closed distal end (31), and the inner cladding has an open proximal end (40) in fluid connection with the second opening of the casing and a distal end (41) provided with at least one communication opening (42) between the inside of the outer cladding and the inter-cladding space. The present ...

Method and apparatus for providing a photobioreactor

The present invention provides for a photobioreactor for the cultivation of photosynthetic microorganisms and comprises a hydraulic circuit through which an aqueous solution containing culture of at least one type of photosynthetic organism circulates and gets exposed to a light source. The photobioreactor also comprises a means for feeding carbon dioxide into the system, a means for oxygen degasification, and a means for injecting nutrients into the system. The hydraulic circuit is comprised of two open receiving channels at the same relative elevation, a set of transparent or translucent tubes which connect receiving channels to each other, and at least one fluid moving device to move the fluid from the first to the second receiving channels through the tubes. Each receiving channel also comprises a directional dam which assists in maintaining two different surface levels of the aqueous solution within the upstream and downstream portions of each receiving channel.

CELL CULTURE INSERT

System and method for movement and timing control

The present invention relates to fluidic systems for controlling one or more fluids and/or one or more reagents. These systems can be used in combination with one or more devices for assaying, processing, and/or storing samples. In particular, the systems and related methods can allow for dispensing fluid in a controlled manner and/or introducing pause(s) when implementing assays or processes.

Continuous culture Apparatus With Mobile Vessel and Producing a Culture in a Continuous Manner

Abstract A device for growing living cells in a continuous manner, comprising: a flexible tubing containing culture medium and a surface in which the living cells can grow on in suspension or not in suspension, cohesive or not, wherein the tubing is transparent or translucent to measure turbidity; and a system of clamps, each capable of open and closed positions, the clamps being positioned so as to be able to divide the tubing into: i) an upstream region containing unused culture medium; ii) a downstream region containing spent culture medium; and iii) a growth chamber region for growing said cells disposed between the upstream and downstream regions; wherein the system of clamps is constructed and arranged to open and close so as to clamp off and define the growth chamber region of the tubing between the upstream and downstream regions of the tubing, and to cyclically redefine the growth chamber region of the tubing so that a first portion of the previously defined growth chamber region ...

DEVICE FOR MAKING A CULTURE OF MICRO-ORGANISMS

CAPILLARY

The present invention provides plastic capillaries having an optimized volume-to--surface ratio that are suitable for rtPCR applications. Moreover, the present invention provides a new method for the production of said rtPCR plastic capillaries. In more detail, the present invention provides plastic capillaries that are produced by a 2-step molding process.

MULTILAYER TISSUE CULTURE VESSEL

The present invention discloses a vessel for culturing cells which includes: a bottom including a base with an upwardly extending wall at least partially bounding the base of the bottom; a top including a base with a downwardly extending wall at least partially bounding the base of the top; a tubular neck with an opening defined therein; and, one or more shelves, wherein, each shelf includes a base with an upwardly extending wall at least partially bounding the base of the shelf. The upwardly extending wall of a first shelf adjoins the downwardly extending wall of the top with the first shelf being located intermediate the bottom and the top. The base of each of the shelves having at least one aperture formed therein. The bottom, the top and the one or more shelves collectively define an enclosed volume for culturing cells. The tubular neck extends from the vessel with the enclosed volume being accessible by the opening in the tubular neck. Advantageously, this vessel provides high volume ...

COLLECTION TUBES APPARATUS, SYSTEMS, AND METHODS

Methods of producing collection tubes are presented. The methods include providing a separator substance that can rapidly polymerize in a short time to a desired hardness and disposing the separator substance within the lumen of the tube. The separator substance is formulated to have a density between an average density of a serum fraction of whole blood and a cell-containing fraction of whole blood, and to be flowable with whole blood. Upon centrifugation of a tube having blood, the separator substance forms a barrier between the whole blood fractions. The tube and barrier maintain stability of one or more analyte levels, including potassium and glucose, within 10% of their initial values before centrifugation for at least four days.

SUBSTRATE-CONTAINING TEST CONTAINER FOR THE CULTIVATION AND INSPECTION OF MICRO ORGANISMS

STERILIZABLE, GAS PERMEABLE CONTAINER FOR USE IN CULTURING LIVING CELLS

A container for use in culturing living cells includes an enclosable portion, or base, and a cover for enclosing the enclosable portion, a path defined between the junction of the enclosable portion and the cover and a gas permeable filter positioned within the path. Gases flow into and out of the container through the filter. Vapor phase hydrogen peroxide is degraded into water vapor and oxygen by a material associated with the filter to prevent hydrogen peroxide from entering the container.

MICROORGANISM CULTURING DEVICE

A device for culturing microorganisms includes a vessel having a mouth for containing a culture medium and a stopper member for hermetically sealing the vessel. The stopper member is provided with a through hole on its peripheral wall for communicating with the inner atmosphere of said vessel.

ANAEROBIC CULTURE COLLECTING AND TRANSPORTING APPARATUS

A culture collecting and transporting apparatus comprising a closable container having a collected culture receiving depot therein, means in the container for supplying a culture medium to a culture placed on the depot, and chemical means in the container selectively activated after a culture is placed on the depot, said chemical means when activated supplying gaseous carbon dioxide, and a reducing agent system effective for reducing oxygen, at least in the container space surrounding the collected culture. The reducing agent system may comprise a reducing gas and a catalyst for inducing reaction of the oxygen with the reducing gas. The reducing gas may be hydrogen.

DEVICE FOR MAKING A CULTURE OF MICRO-ORGANISMS

A device for making a culture of micro-organisms has a container having an open end and means to seal the open end of the container. A second seal divides the container into a first compartment for a liquid culture medium remote from the open end of the container and a second compartment adjacent the open end of the container. A removable elongated member in the container has a portion extending through the second compartment and into said first compartment and removably engaging the second seal to seal liquid culture medium in the first compartment. A culture medium secured to a portion of said elongated member lies in the second compartment. Preferably the side edges of the elongated member are tapered adjacent its inner end and a portion of the elongated member in the second compartment carries one or more solid culture mediums. Advantageously the second seal member is tapered to guide the elongated member through the center thereof.

APPARATUS AND METHOD FOR THE DETECTION OF MICROBIAL PATHOGENS

CELL CULTURE VIAL

... 578893 26260-FF An apparatus for maintaining a biological sample on a support is disclosed. The apparatus comprises a hollow chamber with internal and external surfaces and a first and second end. The first end is open and is adapted for attachment to the first closure means. The second end is either closed or open. If open, the second end is adapted for attachment to a second closure means. The apparatus provides means for maintaining the support in contact with a liquid medium at all times regardless of the orientation of the apparatus. In a preferred embodiment, the apparatus provides means for maintaining the original orientation of a non-integral support regardless of the orientation of the apparatus. The apparatus is useful for isolating and culturing microorganisms.

TISSUE CULTURE FLASK

A tissue culture flask includes a container main body comprising a box having a bottom surface for supporting a substance to be cultured, a side surface, and an upper surface. A cylindrical neck is provided on the side surface of said container main body to communicate with the interior of said container main body, and extends obliquely upward with respect to the bottom surface and defining a first opening at a distal end thereof and a second opening at a proximal end thereof. In the flask, (1) a first angle defined by (a) a first straight line connecting a given point on the bottom surface including an edge thereof and a given point on an edge of the first opening and (b) a second straight line on the inner circumferential wall and connecting a given point on an edge of the first opening and a given point on an edge of the second opening is not greater than (2) a second angle defined by (c) said second straight line and (d) the longest third straight line connecting a given point on an ...

CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL, ALLOWING SELECTION OF FITTER CELL VARIANTS

Method and device that increases the rate of reproduction (through increased speed of reproduction and/or increased reproductive yield) of living cells in suspension or of any culturable organisms through the process of natural selection, said device comprising: a) a flexible, sterile tube (7) containing culture medium, b) a system of movable gates (clamps) (3, 4, 5) that divide the tube (97) into separate regions containing spent culture (downstream region), growing culture (growth chamber), and fresh growth medium (upstream region), c) a means of moving the gates and the tubing (13) such that a portion of the growth chamber and the associated culture can be clamped off and separated from the growth chamber, and such that a portion of fresh tubing containing unused medium can be joined with a portion of the culture and associated medium already present in the growth chamber.

APPARATUS FOR RUPTURING A SEALED, FRANGIBLE CONTAINER

TRIPHASIC MYCOPLASMATALES CULTURE DEVICE AND METHOD

METHODS AND COMPOSITIONS FOR PRODUCING CANNABINOIDS

Disclosed herein are methods of producing one or more cannabinoid compounds, expanding cells that produce the compounds, cannabinoid compounds produced by the methods, pharmaceutical compositions comprising the cannabinoid compounds, and methods of producing and utilizing the compounds and compositions.

FLUID TRANSFER METHOD AND DEVICE

DISSOLVABLE FILMS AND METHODS INCLUDING THE SAME

... ²²²A method includes providing a container, introducing a substance into the ²container, and introducing a readily dissolvable film into the container such ²that the dissolvable film overlies the substance within the container. An ²alternative method includes providing a container, providing a readily ²dissolvable film, the film comprising a substance carried by the film, and ²introducing the film into the container.² ...

CENTRIFUGE TUBE ASSEMBLY

... ²²²A centrifuge tube assembly (10) includes an elongate tubular receptacle (12) ²and a cap (14) that attached to the receptacle. The cap includes a pair of ²ports (32), (34) formed therethrough for communicating with an interior of the ²tubular receptacle. A pair of aspiration pipes (36), (38) having different ²lengths are engaged with the respective ports and extend longitudinally ²through the cylindrical receptacle. One of the pipes extends to a point ²proximate the bottom of the tubular receptacle and the other pipe extends to a ²level spaced above the bottom of the tube.² ...

CONTINUOUS CULTURE APPARATUS WITH MOBILE VESSEL, ALLOWING SELECTION OF FITTER CELL VARIANTS AND PRODUCING A CULTURE IN A CONTINUOUS MANNER

A method and device for growing plant, animal or stem cells in a continuous manner.

A METHOD TO INCREASE DISSOLVED OXYGEN IN A CULTURE VESSEL

Basing on study of a previous discovered effective bioreactor system, a m ethod to increase culture medium dissolved oxygen is disclosed. This method together with addition of an optimal mixing forms a theoretical foundation f or effective bioreactor design and prototype construction.

SYSTEM OF MULTIPLE BAGS AND METHOD FOR THE PREPARATION OF HEMOCOMPONENTS

The present invention relates to a biomedical device for the production, storage, traceability and administration of blood components.

PERFUSION CULTURING METHODS AND USES THEREOF

Provided herein are improved methods of culturing a mammalian cell that include providing a conical container containing a mammalian cell suspended in about 4% to about 80% of the volume of the container of a first liquid culture medium; incubating the container for a period of time at about 31°C to about 40°C at a reactor angle of about 5 degrees to about 85 degrees (e.g., about 40 degrees to about 55 degrees) from horizontal and with an agitation of about 20 revolutions per minute (RPM) to about 1000 RPM; and continuously or periodically, during the period of time, removing a first volume of the first liquid culture medium that is substantially free of mammalian cells and adding to the first liquid culture medium a second volume of a second liquid culture medium, wherein the first and second volumes are about equal, and methods of culturing a mammalian cell that include culturing in a gradient perfusion process a mammalian cell suspended in a liquid culture medium under conditions that ...

STERILIZABLE PHOTOPOLYMER SERUM SEPARATOR

Sterilizable separator tubes and methods of utilization of same are presented. Methods include providing a separator tube containing a separator substance that can be polymerized to a desired hardness following sterilization using radical generating radiation. The separator substance is formulated to have a density between that of the average densities of separable fractions derived from a sample fluid such as blood, and to be flowable. Upon centrifugation of a separator tube containing a fluid sample, the separator substance forms a barrier between the fractions. The barrier subsequently hardens to form a solid barrier when triggered by a suitable energy source.

SYSTEMS, APPARATUSES AND METHODS FOR CULTIVATING MICROORGANISMS AND MITIGATION OF GASES

Systems, apparatuses, and methods are provided for cultivating microorganisms. In one example, a system may include a plurality of containers for cultivating microorganisms therein. Each container may be adapted to contain water and may include media disposed therein and at least partially submerged in the water. The media may be adapted to support microorganisms during cultivation and a concentration of microorganisms supported by the media may be higher than a concentration of microorganisms suspended in the water.

CONTAINER FOR CULTIVATING AGRICULTURAL OR BIOLOGICAL MATERIAL

The invention relates to a container (1) for at least cultivating for instance an agricultural or a biological material, the container comprising: a space (7) defined therein for in use accommodating at least the material arranged in the space,- at least one opening providing access to the space; and a closing means (5,9) associated in use with the opening to selectively inhibit access to the space, whereby the closing means exhibit a different permeability during and after initial cultivation.

COLLECTION AND ASSAY DEVICE FOR BIOLOGICAL FLUID

A device for collecting and analyzing a biological fluid is provided. The device contains a sample collecting section having at least one collection pad for collecting a sample of the biological fluid, a sample accommodating section operatively engageable with the sample collecting section for extracting and accommodating the sampled collected by the sample collecting section, a sample analyzing section, disposed within the sample accommodating section and having a sample analyzing means in fluid communication with the sample extracted and accommodated within the sample accommodating section.

COMPOSITIONS FOR FECAL FLORAL TRANSPLANTATION AND METHODS FOR MAKING AND USING THEM AND DEVICES FOR DELIVERING THEM

The invention provides compositions, formulations, pharmaceuticals, devices, containers a nd delivery vehicles, used for the transplantation of fecal flora. Methods for the use and manufacture of the compositions, formulations, pharmaceuticals, devices, containers and delivery vehicles are also included. The fecal transplantation is achieved via oral ingestion, nasogastric infusion or enema. The fecal transplantation is used for the amelioration, stabilisation, treatment and or prevention of a disease, an infection, poisoning or condition having a bowel dysfunction component or side-effect causing constipation, abdominal pain, or diarrhea in a human or an animal.

PHOTOBIOREACTORS AND CULTURE BAGS FOR USE THEREWITH

There is provided a culture bag for use in a photobioreactor. The bag can comprise at least one wall having at least one inlet disposed at a first end portion of the at least one wall or adjacently thereto. The at least one wall defines an internal chamber for receiving a culture medium. The bag also comprises at least one injector for injecting a gas inside the bag, the at least one injector being disposed at a second end portion of the at least one wall or adjacently thereto. The bag also comprises at least one outlet for harvesting a content of the bag, the at least one outlet being disposed at the second end portion of the at least one wall or adjacently thereto. The bag can be translucent or transparent and be effective for holding and sealingly maintaining the culture medium inside the bag and inside the photobioreactor. There is also provided a photobioreactor and a photobioreator modular system.

CELL CULTURE INSERT

Enhanced Detection of Microorganisms in Samples

This invention describes a method for the rapid identification of the presence of microorganisms in a sample. Briefly, in the method of this invention the sample container is divided into a plurality of discrete zones, each of which can be separately monitored for microbial presence. When a sample is placed into this container, detection is simplified as the volume monitored is low (as compared with the sample); since microbial detection is a concentration dependent phenomenon, the speed with which the presence of microbial contamination can be detected is increased.

VESSEL AND CLOSURE ASSEMBLY

A vessel and closure assembly for culturing cells wherein a gas permeable membrane is provided in the closure to allow rapid and uniform equilibration of gases between the atmosphere of the vessel and the atmosphere of the incubator. A plug is provided for occluding passage of gases through the gas permeable membrane when the vessel is removed from the controlled atmosphere of the incubator.

MULTIPLATE SUBCULTURE SOLID MEDIA DEVICES

A multiplate subculture solid media device is provided with a connection for internal flow communication with a liquid medium culture bottle. The device includes a large transparent screw cap on one face thereof to gain easy access to the plurality of media plates for direct examination and sample taking through the growth period. The cap may transparent and of a size allowing continuous examination during the growth period without any opening. Alternatively, the cap internal surface may include the solid media plates so that culture growth activity may be directly observed by easy removal of the cap. As a further feature of the invention, the screw cap may include an integral magnifying lens for enhancing the continuous examination of culture growth.

CULTURE SLIDE ASSEMBLY

The present invention is a chamber slide assembly having a base member, a cooperable vessel comprising a chamber and a neck wherein said vessel is removably attached to the base and means for separating the vessel from the base. Preferably, the assembly may be used for carrying out biological reactions or growth therein, such as growing tissue or cell cultures.

Process for preparing cultures of microorganisms