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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 4253. Отображено 100.
15-03-2012 дата публикации

Device and method for treating cells

Номер: US20120064594A1
Автор: August Johannes Marie Pemen, Eva Stoffels, Johannes Wilhelmus Maria Van Bree
Принадлежит: Eindhoven Technical University

The present invention relates to a device for treating biological cells in an object, the device comprising: —a single winding coil element; —an electrical generator connected to the single winding coil element, the single winding being configured to be positioned essentially around the object; wherein the electrical generator is configured to discharge into the single winding coil element so that the single winding coil element generates a short duration pulsed electromagnetic field by magnetic induction in the single winding coil element, the electromagnetic field having a field strength that is sufficiently high to affect, preferably increase the permeability of cell membranes and/or intracellular membranes of the biological cells contained in the object when in operation the object is placed inside the single winding coil element.

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Номер записи: 1
22-03-2012 дата публикации

A method and a device for the electrical treatment of a plurality of containers

Номер: US20120067639A1
Автор: Andreas Heinze, Andreas WIRTH, Herbert Müller-Hartmann, Ludger Altrogge, Timo Gleissner
Принадлежит: LONZA COLOGNE GMBH

The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device ( 1 ) for making electrical contact with at least one container fitted with electrodes, with at least one receptacle ( 3, 7 ) upon which at least one container can be set, and with at least one contact appliance ( 8 ) for making contact with the electrodes of the container. According to the invention at least two receptacles ( 3, 7 ) are provided, wherein one receptacle ( 7 ) is located at or inside the contact appliance ( 8 ), and wherein both receptacles ( 3, 7 ) and/or the contact appliance ( 8 ) can be moved such that after completion of the movement the other receptacle ( 3 ) is located at or inside the contact appliance ( 8 ).

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Номер записи: 2
12-04-2012 дата публикации

Container with a plurality of reaction spaces and electrodes

Номер: US20120087841A1
Автор: Andreas Heinze, Andreas WIRTH, Herbert Mueller-Hartmann, Ludger Altrogge, Timo Gleissner
Принадлежит: LONZA COLOGNE GMBH

The invention relates to a container ( 1 ) with at least three reaction spaces ( 2 ) which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space ( 2 ) and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode ( 3, 4 ) of a reaction space ( 2 ) is a common electrode ( 3, 4 ) with at least one other reaction space ( 2 ). According to the invention n+x electrodes ( 3, 4, 5 ) are provided, wherein n is the number of reaction spaces ( 2 ), with n≧3 and x is the number of rows, with x≧1.

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Номер записи: 3
31-05-2012 дата публикации

Low-pressure biolistic barrels

Номер: US20120135526A1
Автор: Kenneth Greenberg
Принадлежит: UNIVERSITY OF CALIFORNIA

Low pressure biolistic barrels and biolistic devices including the same are provided. Aspects of the biolistic barrels include the presence of one or more pressure-reducing elements. Also provided are kits which include the biolistic barrels, as well as methods of delivering a molecule to a target site with the biolistic barrels and devices that include the same. The devices and methods described herein find use in a variety of applications, including in vivo and in vitro high-precision delivery applications.

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Номер записи: 4
21-03-2013 дата публикации

Plasma irradiation device for substance introduction and substance introduction method using plasma irradiation device

Номер: US20130071905A1
Автор: Daisuke Seki, Douyan Wang, Hidenori Akiyama, Hisato Saito, Tako Namihira
Принадлежит: Kumamoto University NUC

Disclosed is a liquid culture medium for substance introduction, which is capable of increasing the survival rate of cells after substance introduction as much as possible when the cells are irradiated with plasma for the purpose of introducing a target substance into each of the cells. Specifically disclosed is a liquid culture medium for substance introduction, which is used for the purpose of introducing a predetermined target substance into a cell and enables introduction of the target substance into the cell by having the cell in the liquid culture medium, which contains the target substance, irradiated with a plasma jet. The liquid culture medium contains a damage preventing component that prevents the cell from damage due to the plasma jet.

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Номер записи: 5
28-03-2013 дата публикации

DEVICE COMPRISING A CONDUCTIVE SURFACE AND A CONDUCTIVE POLYMER FOR ADHESION OF CELLS AND TISSUE

Номер: US20130078701A1
Автор: Berggren Magnus, Jager Edwin, Karlsson Roger, Konradsson Peter, Persson Kristin, Richter-Dahlfors Agneta, Svennersten Karl
Принадлежит: OBOE IPR AB

The present disclosure relates to a device comprising a conductive substrate surface (), at least one layer of a conductive polymer () deposited on the surface (), a first electrolyte () arranged in contact with the conductive polymer layer, and a counter electrode (), arranged in contact with the first electrolyte (), such that a potential difference can be applied between the conductive substrate () and the counter electrode (). 1. A device comprising:a conductive substrate surface,at least one layer of a conductive polymer,a first electrolyte arranged in contact with the conductive polymer layer, and the conductive polymer, in a first state, before applying the potential difference, exhibits a first adhesive capacity, wherein the conductive polymer layer is substantially attached to the conductive substrate surface; and', 'the conductive polymer, in a second state, subsequent to application of the potential difference, exhibits a second adhesive capacity, whereby at least a portion of the conductive polymer layer is substantially released from the conductive substrate surface., 'a counter electrode, arranged in contact with the first electrolyte, such that a potential difference can be applied between the conductive substrate and the counter electrode, wherein'}2. The device as claimed in claim 1 , wherein a rate of release is controllable by a magnitude of the potential difference applied.3. The device as claimed in claim 1 , wherein the device further comprises an additional material layer claim 1 , arranged between the conductive polymer layer and the first electrolyte.4. The device as claimed in claim 3 , wherein the additional material comprises cells or tissue.5. The device as claimed in claim 4 , wherein the device further comprises a second electrolyte arranged between the conductive polymer layer and the additional material layer.6. The device as claimed in claim 5 , wherein the second electrolyte is a solid electrolyte layer.7. (canceled)8. (canceled)9. ...

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Номер записи: 6
28-03-2013 дата публикации

DEVICE FOR VIRAL INACTIVATION OF LIQUID MEDIA

Номер: US20130078702A1
Автор: Dhanasekharan Muthukumar
Принадлежит: Genzyme Corporation

An apparatus () capable of viral inactivation of liquid media includes at least one coaxial cylinder () constructed of an outer cylinder () and an inner cylinder (), a liquid media inlet (), at least one emitter of type C ultraviolet radiation (), and a liquid media outlet (). The inner cylinder has an outer diameter adapted to form a gap () between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder. The media flows in a substantially cyclonic flow path along the gap. The at least one emitter of type C ultraviolet radiation is placed inside the inner cylinder. The outlet is connected to the outer cylinder at, or proximal to, an end of the outer cylinder opposite the inlet. 1. An apparatus capable of viral inactivation of cell culture media comprising: i) an outer cylinder having a length, an inner diameter, and an outer diameter;', 'ii) an inner cylinder coaxial with the outer cylinder, having a length substantially equal to the length of the outer cylinder and having an outer diameter adapted to form a gap between the outer diameter of the inner cylinder and the inner diameter of the outer cylinder through which the cell culture media flows in a substantially cyclonic flow path along the gap;, 'a) at least one coaxial cylinder comprisingb) a cell culture media inlet connected to the outer cylinder proximal to an end of the outer cylinder and configured to flow the cell culture media along the substantially cyclonic flow path along the gap;c) at least one emitter of type C ultraviolet radiation placed inside the inner cylinder so as to emit the type C ultraviolet radiation towards the cell culture media to be treated with the type C ultraviolet radiation and thereby inactivate viruses in the cell culture media; andd) a cell culture media outlet connected to the outer cylinder proximal to an end of the outer cylinder opposite the inlet.2. The apparatus of claim 1 , wherein the cell culture media flows along the gap at a flow rate in ...

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Номер записи: 7
11-04-2013 дата публикации

Alternating Electric Current Directs, Enhances, and Accelerates Mesenchymal Stem Cell Differentiation Into Either Osteoblasts or Chondrocytes But Not Adipocytes

Номер: US20130089908A1
Автор: Bizios Rena, Creecy Courtney M.
Принадлежит:

A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current. 1. A method for directing , enhancing , and accelerating mesenchymal stem cell functions , comprising the steps of:culturing mesenchymal stem cells within a three-dimensional matrix or scaffold:exposing mesenchy mal stem cells to alternating electric current.2. The method of claim 1 , wherein the mesenchymal stemcell function is differentiation.3. The method of claim 2 , wherein the mesenchymal ate cells are differentiated to either osteoblast or chondrocyte lineages.4. The method of wherein the three-dimensional matrix or scaffold is current-conducting.5. The method of claim 1 , wherein the three-dimensional matrix is a collagen hydrogel.6. The method of claim 1 , wherein the alternating electric current is a current level pertinent to directing claim 1 , enhancing claim 1 , and accelerating the desired mesenchymal stem cell function.7. The method of claim 1 , wherein the alternating electric current is a current in the range from 10 μA to 40 μA.8. The method of claim 1 , wherein the alternating electric current is a frequency pertinent to directing claim 1 , enhancing. and accelerating the target mesenchymal stem cell function.9. The method of claim 1 , wherein the alternating electric current is a frequency of 10 Hz.10. The method of claim 1 , wherein the alternating electric current is a waveform pertinent to directing claim 1 , enhancing claim 1 , and accelerating the target mesenchymal stem cell function.11. The method of claim 1 , wherein the alternating electric current is of a sinusoidal waveform.12. The method of claim 1 , wherein the alternating electric current is for either a continuous or intermittent pattern and a duration of application pertinent to directing ...

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Номер записи: 8
25-04-2013 дата публикации

OIL-BASED MATERIAL-PRODUCING METHOD AND OIL-BASED MATERIAL-PRODUCING APPARATUS

Номер: US20130102047A1
Автор: ISHIZUKA Akinori, Tsukahara Yasunori, YOSHINO Iwao
Принадлежит:

An oil-based material-producing method includes a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves. The oil-based material-producing method may also include a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step. 1. An oil-based material-producing method , comprising a microwave irradiation step of irradiating oil-based material-producing microorganisms with microwaves.2. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves are irradiated in a presence of a microwave responsive material claim 1 , which is either one of a microwave-absorbing material and a microwave-sensitive material.3. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is able to flow.4. The oil-based material-producing method according to claim 3 , wherein the microwave responsive material has a shape for collecting electric field of microwaves.5. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is immobilized.6. The oil-based material-producing method according to claim 2 , wherein the microwave responsive material is at least any one of a dielectric claim 2 , a conductive substance claim 2 , and a magnetic substance.7. The oil-based material-producing method according to claim 1 , further comprising a collecting step of collecting an oil-based material produced by the oil-based material-producing microorganisms after the microwave irradiation step.8. The oil-based material-producing method according to claim 1 , wherein the oil-based material-producing microorganisms are oil-based material-producing microalgae.9. The oil-based material-producing method according to claim 1 , wherein claim 1 , in the microwave irradiation step claim 1 , microwaves having two or ...

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Номер записи: 9
16-05-2013 дата публикации

Methods and devices for manipulation of target cells using a combined application of acoustical and optical radiations

Номер: US20130122564A1
Автор: Boris Krasovitski, Eitan Kimmel, Shy Shoham
Принадлежит: Technion Research and Development Foundation Ltd

A method of applying a nondestructive mechanical force on one or more cells in aqueous environment by inducing heat generated acoustic pressure pulses. The method comprises providing an energy transmission pattern to induce the applying of a desired nondestructive mechanical force on a at least one cell by forming a plurality of heat generated acoustic pressure pulses in an aqueous environment and instructing the radiation of a target area in proximity to the at least one cell in the aqueous environment with light energy according to the energy transmission pattern.

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Номер записи: 10
16-05-2013 дата публикации

FOREIGN GENE TRANSFER METHOD BY ELECTROPORATION TECHNIQUE

Номер: US20130122592A1
Автор: Hayakawa Kiyoshi, HAYAKAWA Yasuhiko
Принадлежит: NEPA GENE CO., LTD.

Provided is a method for transferring an extraneous gene by an electroporation technique, which is applicable to a wide range of animal cells and is extremely remarkably improved in viability and gene transferring rate. Also provided is a method for transferring an extraneous gene by an electroporation technique with high viability and gene transferring rate even in the case where no specialized transferring buffer is used. Also provided are: a method for transferring an extraneous gene by an electroporation technique, which is remarkably improved in viability and gene transferring rate, the method including continuously applying, to an animal cell, a first electric pulse (strong electric pulse) and a second electric pulse (weak electric pulse) under specific conditions; and a method for transferring an extraneous gene by an electroporation technique, in which a liquid medium capable of being used for culturing of the animal cell is used as a transferring buffer. 1. A method for transferring an extraneous gene into an animal cell by an electroporation technique , the method comprising: applying , to the animal cell , a first electric pulse having an electric field strength of at least 300 V/cm such that a total calorie strength is 0.2 to 40 J/100 μL; and applying a second electric pulse having an electric field strength of at least 15 V/cm or more such that a calorie strength per pulse is 0.01 to 5 J/100 μl.2. The method of claim 1 , wherein the applying of the second electric pulse is performed two or more times.3. The method of claim 1 , wherein the applying of the second electric pulse is performed less than one minute after the applying of the first electric pulse.4. The method of claim 1 , wherein the animal cell is a mammalian cell.5. The method of claim 1 , wherein the animal cell is an animal cell suspended in a solution.6. The method of claim 5 , wherein the solution comprises a liquid medium suitable for culturing the animal cell.7. The method of claim 1 , ...

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Номер записи: 11
27-06-2013 дата публикации

Systems and Methods for Increasing Growth of Biomass Feedstocks

Номер: US20130164812A1
Автор: Green Michael Phillip, Nicholas Eckelberry, Sanchez Pina Jose L
Принадлежит: ORIGINOIL, INC.

Methods and systems for developing and bio-refining or processing biomass feedstocks into a spectrum of bio-based products which can be used as a substitute for fossil oil derivatives in various types of product manufacturing processes and/or the production of bio-energy are disclosed. In addition, methods and systems for identifying, measuring and controlling key parameters in relation to specific biomass developing processes and bio-refining processes so as to maximize the efficiency and efficacy of such processes while standardizing the underlying parameters to facilitate and enhance large-scale production of bio-based products and/or bio-energy are disclosed. 1. A method of increasing biomass feedstock production yield by exposing the biomass feedstock to an electromagnetic field.2. A system of increasing biomass feedstock production yield , the system having two or more electrodes being configured to expose the biomass feedstock to an electromagnetic field.3. The system and method of and , wherein the electromagnetic field is of a magnitude lower than that which would cause electrolysis within a liquid medium containing the biomass feedstock.4. A method of increasing growth of photosynthetic organisms in a liquid medium , comprising exposing:providing a liquid medium containing photosynthetic organisms; andexposing the liquid medium to an electromagnetic field, the electromagnetic field having a lower magnitude than that which would cause electrolysis within the liquid medium.5. The method of claim 4 , wherein exposing the liquid medium to an electromagnetic field further comprises disposing at least two electrodes within the liquid medium and providing a voltage differential between the at least two electrodes.6. The method of claim 5 , wherein the voltage differential is less than or equal to 1.23 V when the liquid medium is substantially a salt water medium.7. The method of claim 5 , wherein the voltage differential is less than or equal to 1.8 V when the ...

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Номер записи: 12
18-07-2013 дата публикации

Means of inducing cellular exocytosis and uses thereof

Номер: US20130183369A1
Автор: Furber John Douglas
Принадлежит:

Means, compositions, and uses for triggering exocytosis of lysosomes in living cells. During exocytosis, lysosomes travel to the plasma membrane of the cell and dump their contents outside of the cells, thus removing accumulations of harmful, reactive metabolic by-products, such as lipofuscin. Consequently, cells function better, thus improving their vitality and the vitality of people, animals, and cell cultures. Degenerative diseases can be prevented or reversed. Embodied methods include electrical pulses, sonic vibrations, mechanical pressure, and mixtures of substances, which may include drugs, proteins, antibodies, or liposomes, which can be combined, injected, or administered transdermally or orally. Other embodiments are described and shown. 1. methods of triggering biological signaling pathways that cause exocytosis by lysosomes in living cells.2. methods of using electrical stimulation to trigger said signaling pathways.3. methods of using mechanical pressure or sonic vibrations to trigger said signaling pathways.4. methods of using chemical substances to trigger said signaling pathways.5Trypanosome cruzi. methods of in which said chemical substances are composed of a combination of one or more of the following substances: gp82 from claim 4 , one or more calcium channel agonists claim 4 , one or more pore-forming substances claim 4 , such as streptolysin-O claim 4 , which embed in cellular membranes claim 4 , one or more compounds in the family of tetrahydropyridoethers claim 4 , one or more compounds in the family of cyclodextrine compounds claim 4 , liposomes claim 4 , and materials to enhance distribution and delivery of said substances to membranes of living cells.6Trypanosoma cruzi. methods of in which said chemical substances trigger the same signaling pathway as triggered by the parasite claim 4 , claim 4 , when it contacts a cell to invade it.7Trypanosoma cruzi. methods of in which said chemical substances are harvested from parasites claim 4 , such ...

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Номер записи: 13
25-07-2013 дата публикации

ULTRASONIC CAVITATION DERIVED STROMAL OR MESENCHYMAL VASCULAR EXTRACTS AND CELLS DERIVED THEREFROM OBTAINED FROM ADIPOSE TISSUE AND USE THEREOF

Номер: US20130189234A1
Автор: Victor Steven
Принадлежит: INTELLICELL BIOSCIENCES INC.

Methods of treating using adipose tissue using ultrasonic cavitation to dissociate the fat cells and blood vessels contained within adipose tissue and thereby obtain mesenchymal or stromal vascular fractions for use in human subjects are provided. These methods preferably do not include the use of any exogenous dissociating enzymes such as collagenase and result in increased numbers of the cells which constitute the mesenchymal or stromal vascular fractions (about 10-fold greater) than methods which use collagenase to isolate these cells. 1. A method of recovering a stromal or mesenchymal vascular fraction from blood vessels contained in or proximate to adipose tissue which method comprises treating an adipose tissue containing sample with ultrasonic cavitation under conditions whereby that the fat cells and blood vessels in the sample are exploded or lysed thereby dissociating or releasing substantial numbers of intact stromal vascular or mesenchymal vascular cells from the lysed blood vessels contained in the ultrasonicated adipose tissue while substantially maintaining the viability of the cells constituting the stromal or mesenchymal fraction.226-. (canceled)27. A stromal vascular fraction derived from adipose tissue prepared by the method according to claim 1 , wherein said stromal vascular fraction does not comprise any exogenous collagenase.28. The stromal vascular fraction according to claim 27 , which contains stem or other cells that express at least one protein selected from the group consisting of CD13 claim 27 , CD29 claim 27 , CD34 claim 27 , CD36 claim 27 , CD44 claim 27 , CD49d claim 27 , CD54 claim 27 , CD58 claim 27 , CD71 claim 27 , CD73 claim 27 , Thy-1 or CD90 claim 27 , CD105 claim 27 , CD106 claim 27 , CD151 and SH3.29. The stromal vascular fraction according to claim 27 , which contains stem or other cells that express at least one of CD13 claim 27 , CD29 claim 27 , CD34 claim 27 , CD36 claim 27 , CD44 claim 27 , CD49d claim 27 , CD54 claim ...

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Номер записи: 14
01-08-2013 дата публикации

ELECTROPORATION ELECTRODE CONFIGURATION AND METHODS

Номер: US20130196441A1
Автор: Rubinsky Boris, Troszak Gregory D.
Принадлежит: The Regents of the University of California

Provided herein are the concept that “singularity-based configuration” electrodes design and method can produce in an ionic substance local high electric fields with low potential differences between electrodes. The singularity-based configuration described here includes: an anode electrode; a cathode electrode; and an insulator disposed between the anode electrode and the cathode electrode. The singularity-based electrode design concept refers to electrodes in which the anode and cathode are adjacent to each other, placed essentially co-planar and are separated by an insulator. The essentially co-planar anode/insulator/cathode configuration bound one surface of the volume of interest and produce desired electric fields locally, i.e., in the vicinity of the interface between the anode and cathode. In an ideal configuration, the interface dimension between the anode and the cathode tends to zero and becomes a point of singularity. 1. A singularity-based electrode configuration , comprising:an anode electrode;a cathode electrode; andan insulator disposed between the anode electrode and the cathode electrode, wherein the anode electrode, insulator, and cathode electrode are positioned co-planar with respect to one another.2. The singularity-based electrode configuration of claim 1 , further comprising:an ionic substance in contact with the anode electrode, insulator, and cathode electrode.3. The singularity-based electrode configuration of claim 1 , wherein the insulator separates the anode electrode from the cathode electrode by between five nanometers and five microns.4. The singularity-based electrode configuration of claim 1 , wherein the insulator separates the anode electrode from the cathode electrode by between 50 nanometers and two microns.5. The singularity-based electrode configuration of claim 1 , wherein the insulator separates the anode electrode from the cathode electrode by about 100 nm.6. The singularity-based electrode configuration of claim 1 , ...

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Номер записи: 15
08-08-2013 дата публикации

METHOD FOR INDUCING DIFFERENTIATION OF ADULT STEM CELLS AND NERVE CELLS USING ELECTROMAGNETIC FIELD

Номер: US20130202565A1
Автор: CHO Hyun Jin, Kim Soo Chan, KIM Sung Min, Park Jung-Keug, Seo Young-Kwon, Yoon Hee Hoon, Yoon Moon Young
Принадлежит: Dongguk University Industry-Academic Cooperation Foundation

The present invention relates to a method for differentiation of mesenchymal stem cells or dental pulp stem cells. More specifically, the invention relates to a method for differentiating stem cells to neural cells by applying mesenchymal stem cells or dental pulp stem cells with a low-frequency electromagnetic field. The differentiation method according to the present invention can induce differentiation even with low-cost mediums rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological brain diseases. 18.-. (canceled)9. A method for differentiation of mesenchymal stern cells or adult stem cells into neural cells comprising applying an electromagnetic field to the mesenchymal stem cells or adult stem cells.10. The method of claim 9 , wherein the neural cells comprises astrocytes or oligodendrocytes.11. The method of claim 9 , wherein the electromagnetic field is applied at a frequency of 1 to 1000 Hz.12. The method of claim 9 , wherein the electromagnetic field is applied at a flux density of 1 to 5 mT.13. The method of claim 9 , wherein the mesenchymal stem cells are derived from bone marrow claim 9 , adipose claim 9 , or umbilical cord.14. The method of claim 9 , wherein the adult stem cells are dental pulp stem cells.15. A composition for treatment of neurological diseases comprising the neural cells differentiated by the method of .16. The composition of claim 15 , wherein the neurological diseases is Alzheimer's disease claim 15 , depression claim 15 , Parkinson's disease claim 15 , cerebral infarction claim 15 , cerebral hemorrhage claim 15 , or spinal cord injuries.17. A method of treating a patient comprising administering the neural cells of to the patient. The present invention relates to a method for differentiation of mesenchymal stem cells or adult stem cells. More specifically, the present ...

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Номер записи: 16
08-08-2013 дата публикации

METHOD FOR INDUCING DIFFERENTIATION OF MESENCHYMAL STEM CELLS TO NERVE CELLS USING SOUND WAVES

Номер: US20130202566A1
Автор: CHO Hyun Jin, Jeon Song Hee, Park Jung-Keug, Seo Young-Kwon, Yoon Hee Hoon, Yoon Moon Young
Принадлежит: Dongguk University Industry-Academic Cooperation Foundation

The present invention relates to a method for differentiation of mesenchymal stem cells. More specifically, the invention relates to a method for differentiating mesenchymal stem cells to neural cells by treating the mesenchymal stem cells with low-frequency sound waves. The differentiation method of the present invention can induce differentiation even with low-cost media rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological diseases. 18.-. (canceled)9. A method for differentiation of mesenchymal stem cells into neural cells comprising applying a sound wave to the mesenchymal stem cells.10. The method of claim 9 , wherein the sound wave is applied at a frequency of 1 to 500 Hz.11. The method of claim 9 , wherein the sound wave is applied at a frequency of 30 to 300 Hz.12. The method of claim 9 , wherein the sound wave is applied at an intensity of 0.1 to 5 V.13. The method of claim 9 , wherein the sound wave is applied at an intensity of 0.5 to 3 V.14. The method of claim 9 , wherein the mesenchymal stem cells are derived from bone marrow claim 9 , fat claim 9 , umbilical cord blood claim 9 , or umbilical cord.15. A composition for treatment of neurological diseases comprising the neural cells differentiated by the method of .16. The composition of claim 15 , wherein the neurological diseases is Alzheimer's disease claim 15 , depression claim 15 , Parkinson's disease claim 15 , cerebral infarction claim 15 , cerebral hemorrhage claim 15 , or spinal cord injury.171. A method of treating a patient comprising administering the neural cells of claim to a patient. The present invention relates to a method for differentiation of mesenchymal stem cells. More specifically, the present invention relates to a method for differentiating mesenchymal stem cells into neural cells by applying a sound wave of a ...

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Номер записи: 17
08-08-2013 дата публикации

Microfluidic device for generating neural cells to simulate post-stroke conditions

Номер: US20130203086A1
Автор: Anilkumar Harapanahalli Achyuta, Chris Katnik, Javier Cuevas, Shankar Sundaram
Принадлежит: Charles Stark Draper Laboratory Inc, UNIVERSITY OF SOUTH FLORIDA

This application provides devices for modeling ischemic stroke conditions. The devices can be used to culture neurons and to subject a first population of the neurons to low-oxygen conditions and a second population of neurons to normoxic conditions. The neurons are cultured on a porous barrier, and on the other side of the barrier run one or more fluid-filled channels. By flowing fluid with different oxygen levels through the channels, one can deliver desired oxygen concentrations to the cells nearest those channels.

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Номер записи: 18
08-08-2013 дата публикации

Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture

Номер: US20130203145A1
Автор: Dominko Tanja, Lambert Christopher R., McGimpsey W. Grant, Page Raymond L.
Принадлежит:

The invention provides methods for rapid, continuous generation of cells and cell products using magnetically stabilized three-dimensional tissue culture. The invention also pertains to a continuous flow self-regulating closed system bioreactor system for magnetically stabilized three-dimensional tissue culture. The methods described here do not use traditional solid scaffolding for cell culture. 1. A bioreactor for culturing cells , comprising:a vessel,an apparatus for conveying fluids,magnetic beads having cells attached thereon, andan apparatus for conveying a magnetic field.2. The bioreactor of claim 1 , further comprisingan apparatus for dialysis, andan apparatus for harvesting.3. The bioreactor of further comprising an apparatus for photoelectrochemical processing.4. (canceled)5. (canceled)6. The bioreactor of claim 1 , where the magnetic bead size is 100 nm to 10 μm.7. The bioreactor of claim 1 , wherein more than one type of cell is attached.8. (canceled)9. (canceled)9a. (canceled)9b. (canceled)10. A system for cultivating cells claim 1 , comprising:a bioreactor,magnetic beads having cells attached thereon,an apparatus for conveying a magnetic field, andan apparatus for conveying a continuous flow of fluids, wherein the cells are controlled by the magnetic field and the flow of fluids.11. (canceled)12. (canceled)13. A method for culturing cells in a bioreactor comprising:providing cells attached to magnetic beads,acting upon the cells with a fluid,acting upon the cells with a magnetic field, andallowing the cells to grow and proliferate.14. The method of claim 13 , wherein the cells form a tissue.15. The method of claim 14 , wherein the tissue has a lumen.16. The method of claim 13 , wherein the cells are grown without scaffolding.17. The method of claim 13 , wherein the cells are mammalian cells.18. The method of claim 17 , wherein the mammalian cells are selected from lymphocytes claim 17 , smooth muscle cells claim 17 , stem cells claim 17 , cardiac cells ...

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Номер записи: 19
15-08-2013 дата публикации

METHOD FOR CONTROLLING THE CHAPERONE ACTIVITY OF PEROXIREDOXINS USING IRRADIATION

Номер: US20130210108A1
Автор: An Byung Chull, Bai Hyoung-Woo, Chung Byung Yeoup, Lee Eun Mi, Lee Jae Taek, LEE Seung Sik
Принадлежит:

The present invention relates to a method for increasing chaperone activity by irradiating peroxiredoxin (Prx) proteins. More particularly, the present invention may be useful for preparing recombinant proteins imparting resistance against various environmental stresses by increasing the chaperone activity of peroxiredoxin, since it has been observed that irradiated peroxiredoxin has enhanced chaperone activity characteristics, wherein an α-helix structure decreases while a β-sheet structure increases, from analysis results of a protein structure change and chaperone activity after irradiating two types of peroxiredoxins (2-Cys, 3-Cys) which are two active cysteine motifs of peroxiredoxin. 1. A method for increasing chaperone activity by irradiating peroxiredoxin protein.2. The method for increasing chaperone activity according to claim 1 , wherein the peroxiredoxin protein is 2-Cys peroxiredoxin having two or three cysteine residues.3. The method for increasing chaperone activity according to claim 2 , wherein the peroxiredoxin protein has the amino acid sequence represented by SEQ. ID. NO: 1 or SEQ. ID. NO: 2.4. The method for increasing chaperone activity according to claim 1 , wherein the radiation is selected from the group consisting of gamma ray claim 1 , electron beam (beta ray) claim 1 , and UV.5. The method for increasing chaperone activity according to claim 4 , wherein the dose of gamma ray is 1˜500 kGy.6. The method for increasing chaperone activity according to claim 4 , wherein the dose of gamma ray is 1˜30 kGy.7. The method for increasing chaperone activity according to claim 4 , wherein the dose of UV is 5˜240 W.8. A peroxiredoxin protein with increased chaperone activity prepared by the method of .910-. (canceled)11. A method for increasing chaperone activity by the gamma ray or UV irradiating 2-Cys or 3-cys peroxiredoxin protein having two or three cysteine residues.12. The method for increasing chaperone activity according to claim 11 , wherein ...

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Номер записи: 20
29-08-2013 дата публикации

Light-Activated Chimeric Opsins and Methods of Using the Same

Номер: US20130224821A1
Автор: Deisseroth Karl, Fenno Lief, Hegemann Peter, Prigge Matthias, Yizhar Ofer
Принадлежит: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Provided herein are compositions comprising light-activated chimeric proteins expressed on plasma membranes and methods of using the same to selectively depolarize excitatory or inhibitory neurons. 151.-. (canceled)52. An isolated light-responsive chimeric polypeptide comprising an amino acid sequence having at least about 85% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:1.53. The chimeric polypeptide of claim 52 , wherein the polypeptide is activated by light of a wavelength between about 540 nm to about 560 nm.54. The chimeric polypeptide of claim 52 , further comprising a C-terminal trafficking signal.55. The chimeric polypeptide of claim 54 , wherein the trafficking signal comprises the amino acid sequence KSRITSEGEYIPLDQIDINV (SEQ ID NO:15).56. The chimeric polypeptide of claim 52 , wherein the light-responsive chimeric polypeptide comprises an amino acid sequence having at least about 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:1.57. The chimeric polypeptide of claim 52 , wherein the light-responsive chimeric polypeptide comprises an amino acid sequence having at least about 95% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:1.58. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of .59. The isolated polynucleotide of claim 58 , wherein the nucleotide sequence is operably linked to a promoter.60. The polynucleotide of claim 58 , wherein the polynucleotide is an expression vector.61. The polynucleotide of claim 60 , wherein the expression vector is a viral vector.62. A mammalian cell comprising the polynucleotide of claim 58 , wherein the light-responsive chimeric polypeptide is present in the cell membrane.63. The mammalian cell of claim 62 , wherein the cell is a neuronal cell claim 62 , a muscle cell claim 62 , or a stem cell.64. The mammalian cell of claim 62 , wherein the cell is an excitatory neuronal cell or an ...

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Номер записи: 21
29-08-2013 дата публикации

MODIFICATION OF MICROALGAE FOR MAGNETIC PROPERTIES

Номер: US20130224822A1
Автор: Postier Brad, Sayre Richard
Принадлежит:

Genetically engineered algae strains for biofuels and bioproduct production have improved iron utilization including iron uptake and storage, and exhibit improved growth characteristics, magnetic separation, and magnetic hysteresis induced cell lysis. Pond production algal strain embodiments with high iron scavenging capabilities limit iron availability to contaminating microorganisms and invading species. Accumulation of high iron and other paramagnetic elements content in the form of ferritin enhances the cells magnetic susceptibility improving efficiency of magnetic separation and magnetic hysteresis induced cell lysis. Several genes of the embodiments are capable of improving iron acquisition including the ferritin gene fer1, iron transport gene fea1, and iron reductase gene fre1. Other genes which improve growth in the high iron conditions permitting higher accumulation of iron include radical scavenging enzymes such as superoxide dismutase, peroxidase, catalase, glutathione peroxidase and the Ferritin like DPR/DPS genes which also bind iron and protect DNA from reactive oxygen species. 1. A method of separating genetically modified algae from an associated suspending liquid medium , the method comprising:modifying wild-type algae so that one or more cellular iron assimilation components are enhanced under control of a constitutive or regulated promoter to produce genetically modified algae in an associated suspending liquid medium; and,exposing the genetically modified algae to an associated magnetic field to magnetically separate the genetically modified algae from an associated suspending liquid medium.2. The method of wherein the modifying comprises:modifying the algae to tolerate growth under high iron conditions through introduction of genes involved in alleviating iron stress including reactive oxygen species production.3. The method of wherein the introduction of genes comprises:an introduction of reactive oxygen species responsive genes belonging to ...

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Номер записи: 22
05-09-2013 дата публикации

METHOD AND DEVICE FOR UNIFORMLY TREATING ADHERENT CELLS

Номер: US20130230895A1
Автор: Gleissner Timo, Heinze Andreas, Koblizek Thomas, Mueller-Hartmann Herbert, Wirth Andreas
Принадлежит: LONZA COLOGNE GMBH

The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes wherein at least three electrodes are provided, and wherein at least two electrodes are active electrodes when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes is a potential-free electrode when the voltage is applied. The invention further relates to a device comprising at least three electrodes which are connected to at least one voltage source by means of at least one switching device wherein at least five electrodes are connected to the at least one voltage source by means of four switching devices wherein at least two electrodes are connected to the voltage source by means of a common switching device In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices. 1. Method for applying at least one electric field to adherent cells , in which the electric field is generated by applying a voltage to at least two active electrodes , comprisingproviding at least three electrodes wherein at least two of said electrodes are active electrodes when the voltage is applied for generating a first electric field, and that at least a second electric field is generated, wherein at least one of the two former active electrodes is a floating electrode when the voltage is applied.2. The method according to claim 1 , wherein as many electric fields as necessary are generated during generation of further electric fields to make at least once floating electrodes out of all electrodes that have been ...

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Номер записи: 23
05-09-2013 дата публикации

HIGH-THROUGHPUT SENSORIZED BIOREACTOR FOR APPLYING HYDRODYNAMIC PRESSURE AND SHEAR STRESS STIMULI ON CELL CULTURES

Номер: US20130230907A1
Автор: Ahluwalia Arti, DE MARIA Carmelo, MAZZEI Daniele, VOZZI Giovanni
Принадлежит:

A bioreactor () for subjecting a cell culture () to a hydrodynamic pressure stimulus is described. This hydrodynamic pressure is generated inside at least one culture chamber () by means of variation of the mean distance, with a controlled speed, of a surface relative to the surface on which the culture () is positioned and between which the culture medium () is free to flow. 113235. A bioreactor () for subjecting a culture () to a hydrodynamic pressure , this hydrodynamic pressure being generated inside at least one culture chamber () by means of the variation of the mean distance , with controlled speed , of a surface relative to the surface on which said culture or natural/artificial cell constructs () are positioned and between which a culture medium () is free to flow.3110. A bioreactor () as claimed in claim 2 , wherein said movement of said moving element () can generate said hydrodynamic pressure.4153. A bioreactor () as claimed in claim 1 , wherein said hydrodynamic pressure generates a motion of the culture medium () suitable to determine a controlled shear stress on said culture ().5110. A bioreactor () as claimed in claim 2 , wherein said moving element () consists of a piston.6110. A bioreactor () as claimed in claim 2 , wherein said moving element () consists of a prism having a trapezoidal base.7110. A bioreactor () as claimed in claim 2 , wherein said moving element () consists of a cam.8111710. A bioreactor () as claimed in claim 2 , wherein said bioreactor() can be interfaced with a local hardware () for setting and modifying the motion of each of said moving elements () in real time.912225222abab. A bioreactor () as claimed in claim 2 , wherein each of said culture chamber () is provided with at least one inlet port () and one outlet port () adapted to enable said culture medium () to enter and come out of said culture chamber () and wherein said inlet () and outlet () ports do not affect said hydrodynamic pressure.101458. A bioreactor () as ...

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Номер записи: 24
03-10-2013 дата публикации

METHOD AND ELECTRODE ASSEMBLY FOR TREATING ADHERENT CELLS

Номер: US20130260434A1
Автор: Mueller-Hartmann Herbert, Wirth Andreas
Принадлежит: LONZA COLOGNE GMBH

The invention relates to an electrode assembly in particular for applying at least one electric field to adherent cells, comprising at least two electrodes each having at least one surface which is arranged opposite the corresponding surface of the other electrode wherein an electrically insulating material is arranged at least partially between the surfaces of the electrodes The solution according to the invention allows the electric field to be concentrated in the region of the cells to be treated such that a voltage pulse, or the current produced thereby, flows through the cells without the majority flowing away over the cells unused in the electrolyte. The invention further relates to a method for applying at least one electric field to adherent cells, in which the electric field is generated by applying a voltage to at least two electrodes, the electric field is concentrated on the side of the electrodes which faces the cells and/or is limited to the space between the cells and the side of the electrodes which faces the cells. 1. Electrode arrangement comprisingat least two electrodes which each include at least one area being disposed face to face with the corresponding area of the respective other electrode, wherein an electrically isolating material is at least partially disposed between the areas of the electrodes.2. The electrode arrangement according to claim 1 , wherein at least three electrodes are provided.3. The electrode arrangement according to claim 1 , wherein the electrodes are formed like a plate or pin.4. The electrode arrangement according to claim 1 , wherein the areas are lateral surfaces of electrode plates that are disposed coplanar.5. The electrode arrangement according to claim 1 , wherein the areas are completely separated from each other by the isolating material.6. The electrode arrangement according to claim 1 , wherein the space between the electrodes defined by the areas of the electrodes is completely filled by the isolating ...

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Номер записи: 25
03-10-2013 дата публикации

ELECTROPORATION-INDUCED ELECTROSENSITIZATION

Номер: US20130260435A1
Автор: Pakhomov Andrei G., Pakhomova Olga
Принадлежит: Old Dominion University Research Foundation

Methods of enhancing membrane permeabilization in a cell are provided. A method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the method, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm. 1. A method of enhancing membrane permeabilization in at least one cell , comprising:(a) disposing the at least one cell between a first electrode and a second electrode; and(b) applying a plurality of electrical pulses between the first electrode and the second electrode,wherein the plurality of electrical pulses comprise at least two trains of pulses separated by an interval, and wherein an amplitude of the electrical pulses is greater than about 0.2 kV/cm.2. The method of claim 1 , wherein a duration of time for the plurality of pulses is between about 10 s and 1000 s.3. The method of claim 1 , wherein the amplitude is between about 0.2 kV/cm and 15 kV/cm.4. The method claim 1 , wherein the plurality of electrical pulses comprises less than about 1500 pulses.5. (canceled)6. (canceled)7. The method of claim 1 , wherein the interval is at least 10 s.8. (canceled)9. The method of claim 1 , wherein the interval is between about 10 s and 30 minutes.10. The method of claim 1 , wherein the at least two trains of pulses comprise a first train of one or more electrical pulses and a second train of one or more electrical pulses.11. The method of claim 10 , wherein the first train and the second train are substantially the same.12. The method of claim 10 , wherein the first train and the second train are different.13. The method of claim 10 , wherein a number of electrical pulses in the first train comprises 25% to 75% of the plurality of electrical pulses.14. (canceled)15. The method of ...

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Номер записи: 26
03-10-2013 дата публикации

MOLECULAR DELIVERY WITH NANOWIRES

Номер: US20130260467A1
Автор: Jorgolli Marsela, Park Hongkun, Robinson Jacob, Shalek Alexander k., Sutton Amy
Принадлежит: President and Fellows of Harvard

A molecular delivery device including a plurality of nanowires (e.g., Si NWs) coated with an electrically conductive layer. Also disclosed are methods for delivering a molecule by nanowire-mediated electroporation. 2. The device of claim 1 , wherein each of the nanowires is attached to the surface along a substantially vertical direction to the surface.3. The device of claim 1 , wherein each of the nanowires is formed of a semiconductor claim 1 , a compound semiconductor claim 1 , a metal oxide claim 1 , a metal claim 1 , carbon claim 1 , boron nitride claim 1 , or a combination thereof.4. The device of claim 3 , wherein the semiconductor is silicon.5. The device of claim 1 , wherein each of the nanowires is 20-10 claim 1 ,000 nm in height and 10-500 nm in diameter.6. The device of claim 5 , wherein the height is 100-5 claim 5 ,000 nm and the diameter is 50-250 nm.7. The device of claim 6 , wherein the height is 800-1 claim 6 ,200 nm and the diameter is 70-180 nm.8. The device of claim 1 , wherein the electrically conductive layer is formed of a metal claim 1 , a metal oxide claim 1 , a semiconductor claim 1 , a compound semicoductor claim 1 , a metal nitride claim 1 , or combination thereof.9. The device of claim 1 , wherein the density of the nanowires is 0.05-10 μm.10. The device of claim 9 , wherein the density is 0.1-5 μm.11. The device of claim 10 , wherein the density is 0.2-2 μm.12. A method of delivering a molecule to a cell claim 10 , the method comprising:providing a substrate having a surface and a plurality of nanowires attached to the surface, wherein both the substrate and nanowires are electrically conductive;contacting the nanowires with the cell to allow penetration of one or more nanowires into the cell, wherein the cell is immersed in a bath solution containing the molecule; andapplying a current or voltage waveform between the substrate and an electrode in the bath solution, whereby the molecule enters into the cell through transiently formed ...

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Номер записи: 27
03-10-2013 дата публикации

Method, Device and System for Targetted Cell Lysis

Номер: US20130261683A1
Автор: Dodgson John Robert, Soikum Soiwisa, Thomsen Lars
Принадлежит: GIANTCODE CORPORATION PTE LTD

A method, device and system employs particles, such as nanoparticles, and an electric or electro-magnetic field, to cause cell death in target cells by non-thermal means. The method of causing targeted cell death comprises the steps of: introducing a particle to the interior of a target cell and exposing the target cell to a transient electromagnetic field for a sufficient time interval in order to cause cell death. The invention overcomes problems associated with similar methods as a result of the fact that a smaller electric field is applied because the particle enhances the effect of the electric field in its immediate vicinity, so reducing the field strength needed to achieve cell lysis and thereby reducing the risk of damage to healthy cells that may be in its vicinity. Apparatus for performing the method; as well as techniques of delivering particles and for producing particles are also described. 1. A method of causing targeted cell death by a non-thermal mechanism comprising:introducing a particle to an interior of a target cell; andexposing said target cell to an electric field for a sufficient time interval to cause cell death.2. A method of causing targeted cell death by a non-thermal mechanism according to claim 1 , wherein:said particle in said target cell, and said electric field, are selected so as to cause irreversible electroporation of said target cell.3. A method of causing targeted cell death by a non-thermal mechanism according to claim 1 , further comprising:adhering a second particle on, or adjacent, an exterior surface of said target cell.4. A method of causing targeted cell death by a non-thermal mechanism claim 1 , comprising:introducing at least two particles outside a target cell; andexposing said target cell to a transient electromagnetic field for a time interval sufficient to cause cell death;whereby said at least two particles act co-operatively to cause said cell death.5. A method of causing targeted cell death by a non-thermal ...

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Номер записи: 28
10-10-2013 дата публикации

Methods for Culturing Cells in an Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus

Номер: US20130267003A1
Автор: Goodwin Thomas J., Kushman Moses J.
Принадлежит:

Provided herein are methods for culturing cells, tissues or organoid bodies in the presence of a pulsating alternating ionic magnetic resonance field and models comprising a tissue-like assembly of the cells so cultured. The cells, tissues or organoid bodies are introduced into a culture unit comprising growth and nutrient modules in which the gravity vector of the growth unit is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field. 1. A method for culturing cells , comprising the steps of:introducing cells into a culture unit having a growth module and a nutrient module;randomizing continually the gravity vector of the growth module; andapplying a pulsating alternating ionic magnetic resonance field to the growth module during culturing of the cells.2. The method of claim 1 , wherein the introducing step comprises:placing the cells into the growth module;filling the growth module with growth media;sealing the growth module to remove observable gases;attaching the growth module to the nutrient module; andadding fresh growth medium to the nutrient module.3. The method of claim 1 , wherein the randomizing step comprises:positioning the nutrient module comprising the culture unit into a randomizing adapter; andrandomizing the gravity vector via a randomizing mechanism comprising the randomizing adapter.4. The method of claim 1 , wherein the applying step comprises:positioning the growth module comprising the culture unit into an electromagnetic chamber; andgenerating the pulsating alternating ionic magnetic resonance field in the electromagnetic chamber.5. The method of claim 1 , further comprising the step of:modulating the alternating ionic magnetic resonance field to produce overlapping or fluctuating alternating ionic magnetic resonance frequencies at one or more modal intervals spanning about 6.5 Hz and ranging from about 7.8 Hz to about 59.9 Hz.6. The culture system of claim 5 , wherein the overlapping or ...

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Номер записи: 29
10-10-2013 дата публикации

Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus

Номер: US20130267020A1
Автор: Goodwin Thomas J., Kushman Moses J.
Принадлежит:

Provided herein are a culture apparatus, culture systems and an alternating ionic magnetic resonance electromagnetic chamber for culturing cells, tissues or organoid bodies or for delivering a pulsating alternating ionic magnetic resonance field to an object of interest including the above or to an animal, human or plant. The culture apparatus comprises a culture unit having growth and nutrient modules and a randomizing adapter to continually randomize the gravity vector in the growth module. The culture systems further comprise the alternating ionic magnetic resonance electromagnetic chamber. 1. A culture apparatus for growing cells , comprising:means for containing the cells in a growth environment; andmeans for continually randomizing the gravity vector in the growth environment.2. The culture apparatus of claim 1 , wherein the means for containing the cells is a culture unit comprising a nutrient module and growth module in fluid contact claim 1 , said cells contained within the growth module.3. The culture apparatus of claim 2 , wherein the nutrient module comprises:an open-ended body having a proximal end with a diameter of a length to receive the growth module therein; anda distal end comprising a first sealable opening.4. The culture apparatus of claim 3 , wherein the proximal end of the nutrient module comprises a first gas-permeable membrane with a gas port disposed thereon.5. The culture apparatus of claim 2 , wherein the growth module comprises:a body having a proximal end comprising a second gas-permeable membrane with a plurality of inlet/outlet ports disposed thereon; anda distal end comprising a baffling system and a semi-permeable membrane in fluid contact with the first gas-permeable membrane.6. The culture apparatus of claim 2 , wherein the means for continually randomizing the gravity vector in the growth environment comprises a randomizing adapter having an open proximal end of a diameter sufficient in length to receive the culture unit therein ...

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Номер записи: 30
17-10-2013 дата публикации

COMPOSITIONS AND METHODS FOR CONTINUOUS HARVESTING OF SUSPENSION GROWTH CULTURES

Номер: US20130273630A1
Автор: CROWELL Richard, ZHANG Tianxi
Принадлежит: SOLIX BIOFUELS, INC

Embodiments herein concern compositions, methods and uses for harvesting suspension cultures or decontaminating waters. In certain embodiments, suspension microorganism cultures can comprise algal cultures. In some embodiments, harvesting suspension cultures may include using a composition capable of interacting with the culture in order to separate the culture from a liquid or media. 1. A method for harvesting a suspension of an organism in a liquid , comprising:adding a magnesium agent to the suspension to produce a flocculant comprising the organism and the magnesium agent;separating the flocculant from the liquid; andharvesting the organism.2. The method of claim 1 , wherein the flocculant is produced at a pH about 9.0 to pH about 11.5.3. The method of claim 1 , wherein the magnesium agent is a positively-charged agent comprising a magnesium hydroxide.4. The method of claim 1 , wherein the separating step comprises adding a negatively charged magnetic particle and exposing the flocculant to a magnetic field.5. The method of claim 1 , wherein harvesting the organism occurs after the flocculant is exposed to a reduced pH of about 6.0 to about 7.5.6. The method of claim 1 , further comprising:adding a negatively charged non-magnetic material to the suspension to produce a complex comprising the flocculant and the negatively charged non-magnetic material; andallowing the complex to settle out of the suspension.7. The method of claim 6 , wherein the negatively charged non-magnetic material is selected from silica claim 6 , tungsten claim 6 , aluminum claim 6 , aluminum hydroxides claim 6 , copper claim 6 , allay claim 6 , rare earth materials claim 6 , ceramic materials claim 6 , clay claim 6 , glass claim 6 , calcium claim 6 , and carbon claim 6 , organic materials and composite materials.8. The method of claim 6 , wherein the negatively charged non-magnetic material has a particle size of 0.5 μm to 10 μm.9. The method of claim 6 , further comprising harvesting the ...

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Номер записи: 31
31-10-2013 дата публикации

Producing Algae Biomass Having Reduced Concentration Of Contaminants

Номер: US20130288329A1
Автор: Eckelberry Nicholas, Sanchez Jose
Принадлежит:

The present invention is generally directed to a system for producing an algae biomass and wastewater that have reduced concentrations of contaminants. The algae and wastewater treated by the system of the present invention can be combined in a heterotrophic growth system in which the growth of the algae is increased due to the reduced concentration of contaminants. The algae grown in this manner also has a longer shelf life due to the lack of contaminants within the harvested algae. 1. A method for producing an algae biomass and wastewater having a reduced concentration of contaminants for use in a heterotrophic growth system , the method comprising:supplying a growth medium containing suspended algae into a first flocculation tank, the first flocculation tank comprising a reactor tube for creating an electric field within the growth medium, the electric field causing the algae to flocculate;transferring the growth medium containing flocculated algae into a first flotation tank, the first flotation tank comprising a tank containing a plurality of electrodes which cause the formation of gas bubbles which attach to the flocculated algae and lift the flocculated algae to the surface of the growth medium;removing the floating algae from the surface of the growth media and transferring the removed algae to a heterotrophic growth system;supplying wastewater into a second flocculation tank, the second flocculation tank comprising a second reactor tube for creating an electric field within the wastewater, the second reactor tube including a cathode and an anode, the anode comprising a titanium ruthenium alloy, wherein when the electric field is created, the anode causes the creation of free chlorine within the fluid leading to the oxidation of the ammonia into nitrite and nitrate;after the ammonia is oxidized, transferring the wastewater to the heterotrophic growth system such that the wastewater can act as a food for the growth of the algae in the heterotrophic growth ...

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Номер записи: 32
07-11-2013 дата публикации

System for Optical Stimulation of Target Cells

Номер: US20130295635A1
Автор: Edward Boyden, Feng Zhang, Karl Deisseroth
Принадлежит: Leland Stanford Junior University

Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing a NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell.

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Номер записи: 33
14-11-2013 дата публикации

PRODUCTION OF CELL TISSUE HAVING THREE-DIMENSIONAL STRUCTURE USING ELECTROSTATIC INK JET PHENOMENON

Номер: US20130302872A1
Автор: ITO Yoshihiro, KATAHIRA Kazutoshi, KITAJIMA Takashi, Ohmori Hitoshi, UMEZU Shinjiro
Принадлежит: RIKEN

A method and an apparatus for producing a cell tissue of blood vessels or the like with the use of an electrostatic ink jet phenomenon are provided. A pattern generator for cell tissue patterning on a substrate includes at least one vessel for holding a solution containing materials used for cell tissue formation, a substrate that is an object of patterning, and a pattern generator including a voltage applying unit for applying a voltage to the vessel. The voltage is applied to the vessel by the voltage applying unit to generate an electric field between the vessel and the substrate, causing the solution to be ejected from the vessel as a result of an electrostatic ink jet phenomenon and allowing the ejected solution to adhere to the substrate. 1. A method for producing a cell tissue having a three-dimensional structure in which cells remain alive by patterning a cell tissue on a substrate using the pattern generator for cell tissue patterning on the substrate using an electrostatic ink jet system , which comprisesat least one vessel for holding a solution containing materials used for cell tissue formation,a substrate that is an object of patterning,a voltage applying unit for applying a voltage to the vessel, anda plate electrode that is positioned between the vessel and the substrate, grounded, and perforated, the method comprising:ejecting scaffolds and cells onto the substrate so as to allow the scaffolds and the cells to adhere to the substrate for patterning while allowing the cells to come into contact with the scaffolds; andapplying a voltage of 0.5 kv to 5 kv between the vessel and the plate electrode by the voltage applying unit in order to generate an electric field between the vessel and the plate electrode, causing the solution to be ejected from the vessel as a result of an electrostatic ink jet phenomenon and allowing the ejected solution to pass through a hole formed in the plate electrode and adhere to the substrate,wherein a cell tissue is ...

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Номер записи: 34
21-11-2013 дата публикации

SELECTIVE 3D BIOPATTERNING

Номер: US20130309706A1
Автор: Kruglick Ezekiel
Принадлежит: EMPIRE TECHNOLOGY DEVELOPMENT LLC

Methods, systems, and articles for selective three dimensional (3D) biopatterning are disclosed. A biological target may be imaged and a selected area of the image may define a desired pattern for guiding the emission of EM radiation into the biological target. Two or more groups of photosensitive elements responsive to different activation wavelengths may be provided. The photosensitive elements may be selectively activated on or within the biological target based on location and activation wavelength in order to guide cell differentiation, adhesion of growth factors to a scaffold, release of growth factors, and/or deletion of cells. 1. A method for biopatterning a biological target , the method including:selecting a radiation pattern to be applied to an area of the biological target, the radiation pattern defining at least one shape;adding a first group of photosensitive elements to the biological target, the first group responsive to at least a first activation wavelength;radiating a first light into the area of the biological target in a first shape, the first light including at least the first activation wavelength, and the first shape based at least partially on the selected radiation pattern; andradiating at least a second light into the biological target in a second shape, the second light including the first activation wavelength, the second shape based at least partially on the selected radiation pattern, and the first and second lights defining an intersection within the selected area of the biological target, the intersection having a contour corresponding to the at least one shape,wherein a first one or more of the photosensitive elements are located within the intersection and are activated by the first and second lights, andwherein a second one or more of the photosensitive elements are not located within the intersection and are not activated by the first and second lights.2. (canceled)3. The method of claim 1 , further including:selecting, from an ...

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Номер записи: 35
28-11-2013 дата публикации

ANTI-APOPTOSIS OR ANTI-NECROSIS INDUCTION METHOD

Номер: US20130316429A1
Автор: Godal Tomoyuki, Kodama Shohta
Принадлежит:

Disclosed herein is a method for simply inducing an anti-apoptotic effect and/or an anti-necrotic effect in a living cell with good control without administering any drug. The method includes applying an alternating-current voltage to the living cell so that an electric current of 25 μA or higher but 75 μA or lower flows to induce an anti-apoptotic effect and/or an anti-necrotic effect in the living cell. The living cell used may be a cultured cell. The alternating-current voltage may be applied to a stage member on which a container holding the living cell is placed. 1. A method for inducing anti-apoptosis and/or anti-necrosis in a living cell , comprising applying an alternating-current voltage to the living cell so that an electric current of 25 μA or higher but 75 μA or lower flows.2. The method according to claim 1 , wherein the living cell is a cultured cell.3. The method according to claim 1 , wherein the voltage value of the alternating-current voltage is 10 V or higher but 5 kV or lower.4. The method according to claim 2 , wherein the voltage value of the alternating-current voltage is 10 V or higher but 5 kV or lower.5. The method according to claim 1 , wherein the alternating-current voltage is applied to a stage member on which a container holding the living cell is placed. The present invention relates to a method for inducing an anti-apoptotic effect and/or an anti-necrotic effect in a living cell.A technique to suppress the oxidation of coffee or to maintain the freshness of vegetables is conventionally known in which an alternating-current voltage of 10 V or higher but 5 kV or lower is applied to an object to be treated so that an extremely weak electric current of 1 μA or higher but 1000 mA or lower flows (see Patent Documents 1 and 2). However, the mechanism remains unclear by which the oxidation of food can be suppressed or the freshness of food can be maintained by an extremely weak electric current.Meanwhile, apoptosis is one mode of death of ...

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Номер записи: 36
05-12-2013 дата публикации

Apparatus And Method For Biological Growth Enhancement

Номер: US20130318866A1
Автор: Gunderman, JR. Robert Dale
Принадлежит:

An apparatus and method for biological growth enhancement is disclosed. Organisms that will benefit from the apparatus and method of the present invention include seeds, fungus, bacteria, and the like. In one example, seeds are hydro-primed, exposed to a high voltage electric field, and prepared for germination. The resulting sprouts are larger than those that have not been treated by the apparatus for biological growth enhancement. In addition, the root systems of sprouts treated by the apparatus for biological growth enhancement were more advanced than those that were not treated. Benefits include increased production rate of edible sprouts, seedlings that are able to withstand adverse conditions such as drought at an earlier age, and a reduction in the resources required to grow sprouts and plants. 1. An apparatus for biological growth enhancement comprising:an enclosure having a top, a bottom, three sides and an access door;a first electrode having a generally planar form and disposed, within said enclosure;a second electrode spaced apart from said first electrode;electrode adjustment points to vary the spacing between the first electrode and the second electrode;an electronics module comprising a variable output high voltage power supply having a two conductor output;the two conductor output of the high voltage supply being electrically connected to the first electrode and the second electrode respectively; andat least one removable tray for retaining organisms to be treated; whereasthe removable tray is sized to fit within the enclosure and between the first electrode and the second electrode.2. The apparatus of claim 1 , wherein the electrode adjustment points are slots in at least one side of the enclosure.3. The apparatus of claim 1 , wherein the first electrode is a generally planar stainless steel mesh.4. The apparatus of claim 1 , wherein the second electrode is embedded in the enclosure bottom.5. The apparatus of claim 1 , wherein the electronics module ...

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Номер записи: 37
19-12-2013 дата публикации

CULTURE DEVICE

Номер: US20130337554A1
Автор: AY Chyung, Chang Sheng-Chih, Chang Sheng-Nan, CHEN CHENG-NAN
Принадлежит:

A culture device includes a deformable cell-culture unit that includes a culture membrane and a surrounding wall extending upwardly from a periphery of the culture membrane, and a deformable engaging unit that is connected to the surrounding wall of the deformable cell-culture unit, that is adapted to engage a stretching device, and that has a hardness larger than that of the cell-culture unit. 1. A culture device adapted to engage a stretching device , comprising:a deformable cell-culture unit that includes a culture membrane and a surrounding wall extending upwardly from a periphery of said culture membrane; anda deformable engaging unit that is connected to said surrounding wall of said deformable cell-culture unit, that is adapted to engage the stretching device, and that has a hardness larger than that of said cell-culture unit.2. The culture device of claim 1 , wherein said surrounding wall has two opposite side walls claim 1 , said engaging unit including two connecting portions connected to said two opposite side walls.3. The culture device of claim 2 , wherein each of said connecting portions has upper and lower surfaces and is formed with upper and lower grooves that are indented inwardly from said upper and lower surfaces claim 2 , respectively claim 2 , and that extend along a longitudinal direction of said side walls of said surrounding wall claim 2 , the stretching device including a clamping unit that is retained in said upper and lower grooves of each of said connecting portions and that has a length in said longitudinal direction claim 2 , the length of the clamping unit being the same as that of said upper and lower grooves of each of said connecting portions.4. The culture device of claim 3 , wherein said upper and lower grooves of each of said connecting portions are tapered inwardly from said upper and lower surfaces of each of said connecting portions claim 3 , respectively.5. The culture device of claim 3 , wherein each of said connecting ...

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Номер записи: 38
19-12-2013 дата публикации

Method and Apparatus for Patterning Cells

Номер: US20130337565A1
Автор: Albert J. Banes, Chris James Wimmer, Colin Patrick Frazier
Принадлежит: MedTrain Technologies LLC

The present invention is directed to tissue engineering and, more particularly, to devices and methods that are used to pattern or deposit cells to simulate a tissue type in two dimensions or in three dimensions or a cell migration device, a materials testing device for cell proliferation, migration or cell seeder for the Bioflex® flexible bottom culture plates or comparable culture plates. The present invention operates by providing negative pressure to create multiple troughs or indentations for cells to attach and grow on or in the Bioflex® flexible bottom culture plates. The present invention is also directed to a device and method for simulating a tissue wound using the above devices.

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Номер записи: 39
26-12-2013 дата публикации

Reaction Device

Номер: US20130341322A1
Автор: Katsuyoshi Tabuse, Minoru ONCHI
Принадлежит: SUNNY ENGINEERING Co Ltd

A reaction device including a microwave oscillation means for generating microwaves; holding containers for individually holding multiple samples collected from collection targets (e.g., human targets, etc.); a microwave irradiation container on which each of the holding containers can be individually loaded; a temperature sensor for detecting the temperature of a sample held in a holding container or of the interior of the microwave irradiation container; and a microwave control means for varying the microwaves generated by the microwave oscillation means on the basis of the temperature detected by the temperature sensor. The microwave irradiation container includes: a microwave introduction port for introducing the microwaves generated by the microwave oscillation means into the microwave irradiation container; and ring-shaped patterns for irradiating each of the holding containers with the microwaves introduced from the microwave introduction port.

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Номер записи: 40
26-12-2013 дата публикации

DEVICE AND METHOD FOR CONTROLLING NERVE GROWTH

Номер: US20130344559A1
Автор: Engeberg Erik Daniel, Willits Rebecca Kuntz
Принадлежит: THE UNIVERSITY OF AKRON

A nerve growth chamber includes a stimulation vessel defined by one or more sidewalls; at least one anode and at least one cathode positioned in the sidewall or sidewalls of the stimulation vessel; a conductive medium in the stimulation vessel; nerve tissue in the stimulation vessel; and a signal generator connecting the at least one anode and the at least one cathode and activated to generate a periodic AC signal at a desired frequency and amplitude, the periodic AC signal traveling through the conductive medium and affecting the growth of the nerve tissue. A method in accordance with the operation of the apparatus is also provided. 1. An apparatus for controlling the growth of nerve tissue , the apparatus comprising:a. a stimulation vessel defined by one or more sidewalls;b. at least one anode and at least one cathode positioned in said one or more sidewalls;c. a conductive medium in said vessel;d. nerve tissue in said vessel; ande. a signal generator connecting said at least one anode and said at least one cathode and activated to generate a periodic AC signal at a desired frequency and amplitude, said periodic AC signal traveling through said conductive medium and affecting the growth of said nerve tissue.2. The apparatus of claim 1 , wherein the conductive medium is phosphate buffer saline.3. The apparatus of claim 1 , further comprising nerve growth factor in said stimulation vessel.4. The apparatus of claim 1 , further comprising a plurality of stimulation vessels.5. The apparatus of claim 4 , wherein said plurality of stimulation vessels are controlled by said signal generator.6. The apparatus of claim 1 , further comprising a ceiling relative to said one or more sidewalls claim 1 , and at least one of an anode or cathode in said ceiling and connected to said signal generator.7. The apparatus of claim 6 , further comprising a floor relative to said one or more sidewalls claim 6 , and at least one of an anode or cathode in said ceiling and connected to said ...

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Номер записи: 41
26-12-2013 дата публикации

Method for localized photo-irradiation of biological tissues to stimulate tissue regeneration or repair

Номер: US20130344560A1
Автор: Fox Sherry, Weston Jon
Принадлежит:

This invention relates to a method of delivering light energy to biological tissues for the acceleration of healing of damaged or diseased tissues or regeneration of such tissues. More particularly, the present invention relates to applying various wavelengths of light to articular and non-articular joints, alone, or in conjunction with techniques for restoring or regenerating cartilage, ligament and/or tendons whether in-vitro, in-situ or in-vivo. The present invention also extends to application of photo-irradiation to stimulate enhanced proliferation and site-dependent differentiation of stem cells into mature cells and to stimulate full functioning of mature cells involved in tissue repairs for regeneration of tendons, ligaments, cartilage, bone or muscle, depending on the type of tissue with which the stem cells come into contact. 1. A method for stimulating biological regeneration and tissue repair with photo-irradiation whereby light energy in discrete wavelengths , both visible and invisible , is used to stimulate growth and full function of mature cells and to stimulate site dependent differentiation , proliferation and full function of immature cells.2. A method as defined in to stimulate native dwelling progenitor stem cells found in the surface layers of cartilage to engraft in cartilage lesions as a tissue type normally found in that location of the joint.3. A method as defined in to stimulate transplanted stem cells (autologous or allogeneic) in a cartilage lesion to proliferate claim 1 , site-dependently differentiate into the normal local tissue type claim 1 , and to fully function upon reaching cell maturity.4. A method as defined in to stimulate transplanted chondrocytes (autologous or allogeneic) in a cartilage lesion to proliferate claim 1 , site-dependently differentiate into the normal local tissue type claim 1 , and/or to fully function upon reaching cell maturity.5. A method as defined in to stimulate transplanted cartilage cylinders ( ...

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Номер записи: 42
02-01-2014 дата публикации

METHODS AND DEVICES FOR ELECTRICAL SAMPLE PREPARATION

Номер: US20140004501A1
Автор: Alavie Tino, Khine Aye Aye, Maaskant Robert, Talebpour Samad
Принадлежит:

Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing. 1. A method of electrically processing a liquid within a microfluidic device to release macromolecules from at least one cell within the liquid; an upper planar substrate formed from a thermally insulating material;', 'a lower planar substrate formed from a thermally insulating material; and', 'a side wall having a thickness on a submillimeter scale, wherein said upper planar substrate, said lower planar substrate and said side wall define a channel;', 'an upper electrode provided on an inner surface of said upper planar substrate; and', 'a lower electrode provided on an inner surface of said lower planar substrate;, 'the microfluidic device including flowing the liquid into the channel;', 'applying bipolar voltage pulses between the upper electrode and the lower electrode and electrically heating the liquid to an elevated temperature between approximately 30 degrees Celsius and 250 degrees Celsius;', 'wherein the voltage pulses are applied such that the liquid is heated faster than a timescale of thermal diffusion ...

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Номер записи: 43
02-01-2014 дата публикации

MICROBES, METHODS, AND DEVICES FOR REDOX-IMBALANCED METABOLISM

Номер: US20140004578A1
Автор: Bond Daniel R., Flynn Jeffrey M., Gralnick Jeffrey A., Ross Daniel E.
Принадлежит:

The invention generally relates to methods, devices, and microbes involved in performing redox imbalanced fermentations. In one aspect, the invention provides a device that generally includes an electrode and at least one microbe in electron communication with the electrode. The microbe generally can exhibit increased activity of at least one enzyme involved in converting a substrate to a redox imbalanced product. In another aspect, the invention provides a method for performing redox imbalanced fermentation. Generally, the method includes providing a substrate to a microbe under conditions effective for the microbe to metabolize the substrate to a redox imbalanced product. At least one microbe may be in contact with an electrode. In some cases, metabolic conversion of the substrate to the redox imbalanced product can include transferring electrons between the electrode and the microbe. In other cases, metabolic conversion of the substrate to the redox imbalanced product exhibits a carbon flux from organic substrate to organic product of at least 80%. In another aspect, the invention provides a genetically modified microbe. 1. A device comprising:an electrode; andat least one microbe in electron communication with the electrode and genetically modified to exhibit increased activity, compared to a wild-type control, of at least one enzyme that catalyzes a metabolic step converting a substrate to a redox-imbalanced pathway product.2. The device of wherein the microbe comprises an electron flux microbe.3. A device comprising:an electrode; andat least one microbe in electron communication with the electrode and genetically modified to exhibit increased activity, compared to a wild-type control, of at least one enzyme that catalyzes electron flux across the microbe's outer membrane.4. The device of wherein the microbe comprises at least one heterologous coding sequence derived from an electron flux microbe.5Geobacter, Pelobacter, Desulfuromonas, Desulfuromusa, ...

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Номер записи: 44
09-01-2014 дата публикации

METHANOTHERMOBACTER THERMAUTOTROPHICUS STRAIN AND VARIANTS THEREOF

Номер: US20140011251A1
Автор: Mets Laurens
Принадлежит: The University of Chicago

The invention provides an isolated microorganism that is (a) a microorganism of strain UC 120910, deposited on Dec. 21, 2010, with the American Type Culture Collection (ATCC) under ATCC® Patent Deposit Designation No. PTA-11561, (b) a variant thereof, or (c) a progeny thereof, as further described herein. 111.-. (canceled)12Methanothermobacter. An isolated microorganism that produces at least or about 96 molecules of methane per 100 molecules of carbon dioxide supplied to the microorganism or produces at least or about 17 grams of methane from COper gram of cellular material produced from CO.1376.-. (canceled)77Methanothermobacter. An isolated microorganism that:{'sub': 2', '2, 'a. exhibits a methane production efficiency that is at least or about 25 COmolecules converted to methane per COmolecule converted to cellular material; or'}b. survives in a stationary phase with a doubling time of at least or about 72 hours; orc. exhibits a cell culture density of at least or about 6 mg dry mass of cells/ml culture in a stationary phase; ord. returns to at least 80% of the methane productivity level in the operating state within 20 minutes, after an exposure of at least or about 3 minutes to oxygen or carbon monoxide.7884.-. (canceled)85Methanothermobacter. An isolated microorganism that is{'i': 'Methanothermobacter thermautotrophicus', '(a) a microorganism of strain UC 120910, deposited on Dec. 21, 2010, with the American Type Culture Collection (ATCC®) under ATCC® Patent Deposit Designation No. PTA-11561,'}(b) a variant of (a), or(c) a progeny of (a),{'sub': '2', 'wherein the variant or progeny retains the COconversion phenotypic characteristics of (a).'}86MethanothermobacterMethanothermobacter thermautotrophicus. The isolated microorganism of claim 85 , wherein the variant or progeny expresses a 16S rRNA that has at least 90% sequence identity to the full length of the sequence of 16S rRNA of Delta H (SEQ ID NO: 1).87. (canceled)88. (canceled)89Methanothermobacter. The ...

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Номер записи: 45
16-01-2014 дата публикации

SYSTEMS, METHODS AND APPARATUSES FOR AGGREGATING AND HARVESTING MICROORGANISMS FROM AN AQUEOUS SUSPENSION

Номер: US20140017754A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит: Heliae Development, LLC

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. In some embodiments, the cross-sectional area of the electrical field may be tuned. 1. An apparatus for aggregating microorganisms in an aqueous suspension , comprising:a. at least one cathode;b. at least one anode, disposed opposite the at least one cathode;c. at least one pair of spaced insulators disposed between the at least one cathode and the at least one anode; wherein the channel has a length commensurate with the lengths of the at least one cathode and the at least one anode,', 'wherein the channel defines a fluid flow path,', 'wherein the channel comprises a cross-section comprising a height and a width, and wherein at least one of the height and the width of the cross-section varies over a length of the channel;, 'd. a channel defined between the at least one cathode, the at least one anode, and the at least one pair of spaced insulators,'}e. an electrical power source operably connected to the at least one cathode and the at least one anode, wherein an electrical field is created by applying an electric current from the electrical power source to the at least one cathode and the at least one anode; andf. a separation tank in fluid communication downstream of the channel, wherein the separation tank is configured to collect fluid flow from the fluid flow path defined by the channel.2. The apparatus of claim 1 , wherein at least one of the height and the width of the cross-section increases over the length of the fluid flow path.3. The apparatus of claim 1 , wherein at least one of ...

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Номер записи: 46
16-01-2014 дата публикации

AGGREGATING MICROORGANISMS WITH ELECTRICAL AND ACOUSTIC ENERGY

Номер: US20140017755A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. 1. A system for aggregating microorganisms in an aqueous suspension , comprising: i. at least one first electrical conductor;', 'wherein the at least one first electrical conductor is disposed within the at least one second electrical conductor, such that a channel is defined between an exterior surface of the at least one first electrical conductor and an interior surface of the at least one second electrical conductor and with a cross-section comprising a diameter, the channel providing a fluid flow path for the aqueous suspension;', 'ii. at least one second electrical conductor;'}, 'iii. an electrical power source operably connected to the at least one first electrical conductor and the at least one second electrical conductor, wherein electrical field is created when an electric current is provided from the electrical power source to the at least one first electrical conductor and the at least one second electrical conductor and the aqueous suspension;, 'a. a device configured to apply an electric field to an aqueous suspension, comprising i. a tube configured to contain a flow of the aqueous suspension;', 'ii. at least one transducer coupled to the tube;', wherein the generator transmits an electrical radio frequency signal to the transducer and the transducer converts the electrical signal into an acoustic signal which vibrates the tube and creates a wave with a pressure minima node at a location within the tube;', 'wherein the device configured to apply an electrical field and the device ...

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Номер записи: 47
16-01-2014 дата публикации

ELECTRICAL MICROORGANISM AGGREGATION METHODS

Номер: US20140017756A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. 1. A method for aggregating microorganisms in an aqueous suspension , comprising: i. at least one electrical conductor comprising a conductive material configured as a cathode;', 'ii. at least one second electrical conductor comprising a conductive material configured as an anode, wherein the at least one electrical conductor comprising a conductive material configured as a cathode is disposed opposite the at least one second electrical conductor comprising a conductive material configured as an anode;', 'iii. at least one pair of spaced insulators disposed between the at least one electrical conductor comprising a conductive material configured as a cathode and the at least one electrical conductor comprising a conductive material configured as an anode;', 'wherein the channel defines a fluid flow path for the aqueous suspension, and wherein the fluid flow path comprising at least one of a height, a width, and a diameter which varies along at least a portion of a length of the fluid flow path; and', 'iv. a channel defined between the at least one electrical conductor comprising a conductive material configured as a cathode, the at least one electrical conductor comprising a conductive material configured as an anode, and the pair of at least one pair of insulators, wherein the channel has a length commensurate with the lengths of the at least one cathode and the at least one electrical conductor comprising a conductive material configured as an anode,'}, 'wherein at least one aggregating apparatus differs from at ...

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Номер записи: 48
16-01-2014 дата публикации

PATTERNED ELECTRICAL PULSE MICROORGANISM AGGREGATION

Номер: US20140017757A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may comprise different types of pulses. In some embodiments, the flow path for the aqueous suspension may vary. 1. A method of aggregating microorganisms in an aqueous solution , comprising:a. providing an aqueous solution feed comprising a liquid and microorganisms dispersed therein; and 'i. applying a pulsed electric field to the aqueous suspension, the pulsed electrical field generated by a power source and a pulse generator, wherein said pulse generator produces a pattern of electrical pulses which vary in a pulse type comprising at least one of a pulse amplitude, a pulse duration, a pulse shape, and a pause duration between pulses.', 'b. aggregating the aqueous suspension feed, the aggregating comprising2. The method of claim 1 , wherein the pulse shape is rectangular claim 1 , trapezoidal claim 1 , exponentially decaying claim 1 , unipolar claim 1 , or bipolar.3. The method of claim 1 , wherein the pulse duration ranges from 1 to 1 claim 1 ,000 nanoseconds.4. The method of claim 1 , wherein the pattern of electrical pulses alternates more than one pulse types.5. The method of claim 1 , further comprising adding a chemical aggregating agent to the aqueous solution feed.6. The method of claim 1 , wherein the pattern of electrical pulses utilized by the pulse generator comprises a programmed pattern.7. The method of claim 6 , wherein the programmed pattern is selected by a computerized controller based on at least one of a measured turbidity of the aqueous solution claim 6 , a measured density of the aqueous solution claim 6 , a composition of the aqueous solution claim 6 , and a flow rate of the aqueous solution ...

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Номер записи: 49
16-01-2014 дата публикации

TUBULAR ELECTRO-ACOUSTIC AGGREGATION DEVICE

Номер: US20140017758A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. 1. An apparatus for aggregating microorganisms in an aqueous suspension , comprising:a. a tube configured to contain a flow of an aqueous suspension comprising microorganisms;b. an anode disposed within the tube and with a length parallel to a concentric longitudinal axis of the tube;c. a cathode disposed within the tube, and with a length parallel to the anode forming a gap between the anode and cathode comprising the concentric longitudinal axis of the tube;d. an electrical power source operably connected to the anode and the cathode for creating an electrical field by providing an electric current that is applied between the anode and cathode and the aqueous suspension;e. at least one transducer coupled to the tube; andf. a generator configured to produce and transmit radio frequency signals, wherein the generator transmits an electrical radio frequency signal to the transducer and the transducer converts the electrical signal into an acoustic signal which vibrates the tube and creates a standing wave with a pressure minima node at a location between the anode and cathode.2. The apparatus of claim 1 , wherein the electrical field and acoustic standing wave are applied simultaneously to the aqueous suspension.3. The apparatus of claim 1 , further comprising a piezoelectric vibration energy harvester coupled to the tube and configured to convert vibration energy into electrical current.4. The apparatus of claim 1 , wherein the electrical field is pulsed.5. The apparatus of claim 1 , wherein ...

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Номер записи: 50
16-01-2014 дата публикации

RECTANGULAR CHANNEL ELECTRO-ACOUSTIC AGGREGATION DEVICE

Номер: US20140017759A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. 1. An apparatus for aggregating microorganisms in an aqueous suspension , comprising:a. at least one first electrical conductor configured as a cathode;b. at least one second electrical conductor configured as an anode;c. at least one pair of spaced insulators disposed between the at least one first electrical conductor and the at least one second electrical conductor, the at least one first conductor being disposed opposite the at least one second electrical conductor, such that a channel is defined between the at least one first electrical conductor, the at least one second electrical conductor, and the at least one pair of spaced insulators, the channel providing a fluid flow path for the aqueous suspension;d. an electrical power source operably connected to the at least one first electrical conductor and the at least one second electrical conductor, wherein an electrical field is created by providing an electric current from the electrical power source to the at least one first electrical conductor and the at least one second electrical conductor;e. at least one first transducer coupled to the at least one first electrical conductor;f. at least one second transducer coupled to the at least one second electrical conductor; andg. a generator configured to produce and transmit electrical radio frequency signals, wherein the generator transmits an electrical radio frequency signal to the at least one first and second transducers, and the transducers convert the electrical signal into an ...

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Номер записи: 51
16-01-2014 дата публикации

TUNABLE ELECTRICAL FIELD FOR AGGREGATING MICROORGANISMS

Номер: US20140017760A1
Автор: Kale Aniket, KNIEP Justin S.
Принадлежит:

Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary. In some embodiments, the cross-sectional area of the electrical field may be tuned. 1. An apparatus for aggregating microorganisms in an aqueous suspension , comprising:a. a vessel configured to contain an aqueous suspension of microorganisms and configured for fluid communication with a housing;b. at least one first electrical conductor configured as a cathode disposed within the housing;c. at least one second electrical conductor configured as an anode disposed within the housing;d. at least one third electrical conductor configured as a collector electrode disposed within the housing and adjacent to the at least one first electrical conductor;e. at least one fourth electrical conductor configured as a control electrode disposed within the housing and adjacent to the at least one first electrical conductor, wherein the at least one first electrical conductor is at least partially surrounded by the at least one second electrical conductor such that a channel is defined between an exterior surface of the at least one first electrical conductor and an interior surface of the at least one second electrical conductor, providing a fluid flow path configured for receiving the aqueous suspension from the vessel; andf. at least one electrical power source operably connected to the at least one first electrical conductor, second electrical conductor, third electrical conductor, and fourth electrical conductor, wherein an electrical field is created by providing an electrical current from the electrical power source to the at ...

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Номер записи: 52
16-01-2014 дата публикации

Combining Biological Micro-Objects

Номер: US20140017791A1
Автор: Chapman Kevin T., Khandros Igor Y., Mathieu Gaetan L., Short Steven W., Wu Ming C.
Принадлежит:

Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object. 1. A process of combining biological micro-objects , said process comprising:selecting a first biological micro-object and a second biological micro-object from a plurality of micro-objects in a liquid medium in a micro-fluidic device;grouping said first micro-object with said second micro-object in said liquid medium in said micro-fluidic device; andwhile said first micro-object and said second micro-object are grouped, combining said first micro-object and said second micro-object in said liquid medium to produce a combined biological micro-object.2. The process of claim 1 , wherein:said selecting comprises trapping said first micro-object in a first light trap directed into said micro-fluidic device, and trapping said second micro-object in a second light trap directed onto said micro-fluidic device; andsaid grouping comprises merging said first light trap and said second light trap and thereby forming a merged light trap trapping said first micro-object and said second micro-object.3. The process of claim 2 , wherein said combining comprises moving said merged light trap through a combining region of said micro-fluidic device.4. The process of claim 3 , wherein said combining region comprises a chemical that facilitates said combining.5. The process of claim 2 , wherein said combining comprises moving said merged light trap directly between a first electrode and a second electrode to which a power source is connected.6. The process of claim 2 , wherein:said combining comprises moving said ...

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Номер записи: 53
06-02-2014 дата публикации

RAPID CELL PURIFICATION SYSTEMS

Номер: US20140038171A1
Автор: Hance Kenneth Robert, Metzger Steven W.
Принадлежит: Accelerate Technology Corporation

Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface. 1. A method of purifying at least one of cells and viruses in a sample comprising:adding the sample to a well disposed in a medium;applying an electrical potential across the medium to cause contaminants to enter said medium through one or more walls of said well while retaining the at least one of the cells and/or viruses in said well; andremoving the at least one of the cells and viruses from said well.2. The method of claim 1 , wherein said cells are microorganisms.37-. (canceled)8. The method of claim 1 , wherein said medium comprises at least one of a filter or a hydrogel.9. (canceled)10. The method of claim 8 , wherein said hydrogel comprises at least one of a polyacrylamide or agarose.11. The method of claim 1 , comprising placing a buffer in contact with said medium.12. The method of claim 11 , wherein said buffer comprises histidine and tris(hydroxymethyl)aminomethane.13. The method of claim 1 , further comprising immobilizing the at least one of the cells and viruses.14. The method of claim 13 , wherein the at least one the of cells and viruses are immobilized on a positively charged surface.15. The method of any one of claim 1 , further comprising detecting the at least one of the cells and viruses.16. The method of claim 1 , further comprising adding a chemical agent to the sample to increase a permeability of the medium to the ...

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Номер записи: 54
06-02-2014 дата публикации

METHODS OF USING ULTRASOUND IN TISSUE CULTURE AND TISSUE ENGINEERING

Номер: US20140038257A1
Автор: Subramanian Anuradha, TURNER Joseph
Принадлежит:

The present disclosure describes methods of using ultrasound in tissue culture and tissue engineering, as well as a bioreactor that includes at least one ultrasound transducer. 1. A method of culturing cells or tissue , comprising:exposing cells or tissue in culture to intermittent low-intensity-diffuse ultrasound.2. The method of claim 1 , wherein the intermittent low-intensity-diffuse ultrasound comprises a frequency of from about 1 MHz to about 8 MHz.3. The method of claim 2 , wherein the intermittent low-intensity-diffuse ultrasound comprises a pressure of from about 14 kPa to about 60 kPa.4. The method of claim 2 , wherein the intermittent low-density-diffuse ultrasound comprises a duration of exposure of from about 0.5 min to about 10 mins.5. The method of claim 2 , wherein the intermittent low-density-diffuse ultrasound comprises exposure at an interval of from about 8 times per day up to about 16 times per day.6. The method of claim 1 , wherein the cells are selected from the group consisting of osteoblasts claim 1 , osteoclasts claim 1 , osteocytes claim 1 , chondrocytes claim 1 , hepatocytes claim 1 , islet cells claim 1 , myocytes claim 1 , epithelial cells claim 1 , kidney cells claim 1 , neurons and stem cells.7. The method of claim 1 , wherein the tissue is selected from the group consisting of bone claim 1 , cartilage claim 1 , liver claim 1 , pancreas claim 1 , muscle claim 1 , epithelium claim 1 , kidney claim 1 , uterus claim 1 , ovarian claim 1 , and testes.8. A method of inducing phosphorylation in cells by extracellular signal-regulated kinases 1 and 2 (Erk1/2) claim 1 , comprising:exposing cells in culture to intermittent low-intensity-diffuse ultrasound.9. A method of inducing reorganization of actin in cells claim 1 , comprising:exposing cells in culture to intermittent low-intensity-diffuse ultrasound.10. A bioreactor for culturing cells or tissue claim 1 , wherein the bioreactor comprises at least one ultrasonic transducer configured to ...

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Номер записи: 55
06-02-2014 дата публикации

FLUIDIC IN-LINE PARTICLE IMMOBILIZATION AND COLLECTION SYSTEMS AND METHODS FOR USING THE SAME

Номер: US20140038259A1
Автор: Chen Yong
Принадлежит: BECTON, DICKINSON AND COMPANY

Fluidic sorting systems configured to immobilize, and optionally concentrate and/or analyze, one or more particles within the system are provided. Aspects of the systems include a flow-through chamber and an immobilization component configured to immobilize, preferably reversibly, a particle within the flow-through chamber. Optionally, the systems include an analysis component configured to optically analyze an immobilized particle within the flow-through chamber. In certain aspects, the systems are configured to collect one or more particles from the flow-through chamber for subsequent analysis, experimentation, and/or use. Also provided are methods, components and kits for reversibly immobilizing, and optionally analyzing, one or more particles within a fluidic sorting system. 1. A fluidic sorting system comprising:a flow-through chamber configured to receive a particle in a fluid stream from a catcher tube; anda particle immobilization component configured to reversibly immobilize the particle within the flow-through chamber.2. The fluidic sorting system according to claim 1 , wherein the particle immobilization component comprises a magnetic field generator.3. The fluidic sorting system according to claim 2 , wherein the magnetic field generator comprises a permanent magnet.4. The fluidic sorting system according to claim 2 , wherein the magnetic field generator comprises an electromagnet.5. The fluidic sorting system according to claim 1 , further comprising an analysis component configured to analyze the immobilized particle within the flow-through chamber.6. The fluidic sorting system according to claim 5 , wherein the analysis component comprises a microscope.7. The fluidic sorting system according to claim 6 , wherein the microscope is selected from the group consisting of: a conventional microscope claim 6 , a fluorescence microscope claim 6 , a confocal microscope claim 6 , and a laser scanning microscope.8. The fluidic sorting system according to claim 1 ...

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Номер записи: 56
13-02-2014 дата публикации

CELL CULTIVATION CONTAINER AND CELL CULTURING APPARATUS

Номер: US20140045252A1
Автор: Kobayashi Toyoshige, Matsuoka Shizu, Nakajima Ryota, Nozaki Takayuki
Принадлежит: Hitachi, Ltd.

Provided is a cell cultivation container equipped with electrodes, which enables automatic culturing, observation of automatically cultured cells, and measurement of electrical resistances. A circular electrode () is arranged on a base section or a side surface of a frame body () of a cell cultivation container, in such a manner that allows observation of cells. Additionally, a rod-shaped electrode () is arranged on a lid section () of the cell cultivation container, in such a manner that allows observation of cells. In an alternative configuration, the cell cultivation container has a flow channel () made of an electrically conductive material. An alternating current generator () is connected between the electrode () and the electrode (), and the transepithelial electrical resistances of cells are measured during culturing. This configuration enables automatic culturing, observation of automatically cultured cells, and measurement of electrical resistances. 1. A cell culture container for holding and culturing a cell , the cell culture container comprising:a frame body that holds culture liquid for culturing the cell;a lid that is detachably mounted on the frame body;a first electrode mounted on a bottom or on a side face of the frame body and having a shape enabling a cell observation, anda second electrode mounted on the lid and having a shape enabling a cell observation.2. The cell culture container according to claim 1 ,wherein the first electrode is composed of a circular electrode formed on the bottom of the frame body, andthe second electrode is composed of a rod electrode formed on the lid.3. The cell culture container according to claim 1 ,wherein the lid is formed with a flow channel from which the culture liquid is introduced to the frame body,the first electrode is composed of a circular electrode formed on the bottom of the frame body, andthe second electrode is configured to be capable of energizing the flow channel formed on the lid.4. The cell ...

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Номер записи: 57
13-03-2014 дата публикации

METHOD OF BREAKING DOWN BIOLOGICAL MATERIAL

Номер: US20140070033A1
Автор: Klaseboer Evert, Ohl Claus-Dieter, Ohl Siew-Wan, Ow Siak-Wei Dave, Tandiono Tandiono
Принадлежит:

In various embodiments, a method of breaking down biological material is provided. The method may include providing a sample comprising the biological material in a liquid. The method may further include providing a gas such that the gas forms an interface with the liquid. The method may also include breaking down the biological material by applying acoustic waves in a plurality of sequential bursts to the interface. 1. A method of breaking down biological material , the method comprising:providing a sample comprising the biological material in a liquid;providing a gas such that the gas forms an interface with the liquid; andbreaking down the biological material by applying acoustic waves in a plurality of sequential bursts to the interface.2. The method according to claim 1 ,wherein cavitations are generated by oscillations when the acoustic waves are applied to the interface; andwherein the cavitations are configured to break down the biological material.3. The method according to claim 1 ,wherein the biological material are broken down into biological material fragments.4. The method according to claim 3 , the method further comprising:detecting the biological material fragments.5. The method according to claim 3 , the method further comprising:increasing pressure of the gas to flush the biological material fragments.6. The method according to claim 3 , the method further comprising:decreasing the pressure of the gas after flushing the sample comprising the biological material fragments.7. The method according to claim 3 , the method further comprising:providing a subsequent sample comprising a subsequent biological material after flushing the sample comprising the biological material fragments.8. The method according to claim 1 ,wherein a first burst of the plurality of sequential bursts is separated from a second burst of the plurality of sequential bursts by a time interval.9. The method according to claim 1 ,wherein the interface is formed in a main channel ...

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Номер записи: 58
27-03-2014 дата публикации

MAGNETIC THREE-DIMENSIONAL CELL CULTURE APPARATUS AND METHOD

Номер: US20140087440A1
Автор: BECKER JEANNE L., COFFIN SUSAN B.
Принадлежит: UNIVERSITY OF SOUTH FLORIDA

A culture apparatus and method for growing cells and tissue in a three-dimensional configuration harnesses magnetic, paramagnetic, ferromagnetic and diamagnetic forces. The cells or tissue are grown with magnetized core particles and are suspended via magnetic forces in a native, non-restricted, three-dimensional configuration while being maintained in a normal gravity (1 g) growth environment in the absence of rotational alteration of the gravity vector. 122-. (canceled)23. An apparatus comprising:a first diamagnet;a second diamagnet disposed in transversely spaced apart relation to said first diamagnet; andan upper lifter magnet disposed in abutting relation to said first diamagnet.24. The apparatus of claim 23 , wherein said apparatus further comprises a cell culture chamber; and wherein said culture chamber is positioned in sandwiched relation between said first and second diamagnets.25. The apparatus of claim 24 , wherein said cell culture chamber contains a cell culture medium and a plurality of magnetized core particles.26. The apparatus of claim 25 , wherein said cell culture chamber includes biological cells to be cultivated.27. The apparatus of claim 24 , wherein said cell culture chamber is formed of a gas permeable material.28. The apparatus of claim 24 , further comprising:an inlet port formed in said cell culture chamber at a first end of said cell culture chamber; andan outlet port formed in said cell culture chamber at a second end of said cell culture chamber, said second end being spaced apart from said first end.29. The apparatus of claim 25 , wherein said magnetized core particles are coated with a cellular adhesive material.30. The apparatus of claim 25 , wherein said magnetized core particles are coated with a collagen component.31. The apparatus of claim 25 , wherein said magnetized core particles are coated with a matrix component.32. The apparatus of claim 31 , wherein said matrix component is non-biodegradable.33. The apparatus of claim 31 ...

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Номер записи: 59
03-04-2014 дата публикации

METHODS AND APPARATUSES FOR ISOLATING AND PREPARING STEM CELLS

Номер: US20140093482A1
Автор: Paspaliaris Bill, Thornton James Andrew Farrant
Принадлежит: AdiStem, Ltd.

The present invention relates to a method of preparing a population of stem cells for autologous implantation to a subject. The cells are activated by irradiating the cells with one or more wavelengths of yellow and red and/or green light. In particular, the cells are irradiated with 575-595 nm (5-20 mW), and 630-635 nm or 660-670 nm (10-100 mW) and/or 510-540 nm (10-60 mW) of monochromatic light for 30-60 mins. Preferably the stem cells are adipose-derived stem cells. Also the invention relates to therapeutic applications of the activated stem cells. 1. A method of preparing a population of stem cells for autologous implantation to a subject , comprising activating the stem cells by irradiating the cells with one or more wavelengths of yellow and red and/or green light.2. The method of wherein yellow and red and green light is used.3. The method of wherein the cells are irradiated with 575-595 nm (5-20 mW) claim 1 , and 630-635 nm (10-100 mW) and/or 510-540 nm (10-60 mW) of monochromatic light for 30-60 mins.4. The method of wherein the cells are irradiated with 595 nm (20 mW) claim 3 , 635 nm (60 mW) and 535 nm (60 mW) of monochromatic light claim 3 , for 30-60 mins.5. The method of claim 1 , comprising a further initial step of processing a sample of tissue from the subject to obtain the population stem cells to be irradiated.6. The method of wherein the stem cells are adult stem cells.7. The method of wherein the adult stem cells are adipose-derived stem cells (ADSC); dermal stem cells; hematopoietic stem cells; mammary stem cells; mesenchymal stem cells; endothelial stem cells; neural stem cells; neural crest stem cells; or testicular stem cells.8. The method of wherein the stem cells are ADSC and the tissue is adipose tissue.9. The method of wherein the stem cells are exposed to one or more growth factors.10. The method of wherein the growth factors are Epidermal Growth Factor (EGF); Platelet-Derived Growth Factor (PDGF); Fibroblast Growth Factor (FGFs); ...

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Номер записи: 60
07-01-2016 дата публикации

SYSTEM FOR PRODUCING TISSUE-ENGINEERED MATERIAL

Номер: US20160002587A1
Автор: Chen Chao-lin, Chung Chung-Jen, GUO How-Ran, HU Jin-Jia, LEE Yung-Chun
Принадлежит:

A system for producing a tissue-engineered material includes a hollow member and a mechanical stimulating unit. The hollow member is adapted to be implanted in a peritoneal cavity, and is to be positioned in the peritoneal cavity in a manner that a part of the hollow member contacts an inner wall surface of the peritoneal cavity for enabling formation of a biological tissue that encapsulates the hollow member. The mechanical stimulation unit is coupled to the hollow member and configured to provide a periodic mechanical stimulus to the biological tissue by periodically causing the hollow member to expand and contract. A method for producing the aforesaid tissue-engineered material is also disclosed. 1. A system for producing a tissue-engineered material in a peritoneal cavity of a living subject , said system comprising:a hollow member adapted to be implanted in the peritoneal cavity, wherein said hollow member is made of a biocompatible and fluid-impermeable material that is expansible and contractible and is to be positioned in the peritoneal cavity in a manner that a part of said hollow member contacts an inner wall surface of the peritoneal cavity for enabling formation of a biological tissue that encapsulates said hollow member; anda mechanical stimulation unit coupled to said hollow member and configured to provide, during a stimulation session, a periodic mechanical stimulus to the biological tissue being formed on said hollow member by periodically introducing a fluid to flow into and out of said hollow member to cause said hollow member to expand and contract accordingly.2. The system as claimed in claim 1 , wherein said hollow member has an elastic segment that has opposite ends claim 1 , that is elastically deformable claim 1 , and that is to be disposed in the peritoneal cavity claim 1 , and a pair of securing subcutaneous segments that extend from and are in fluid communication with said opposite ends of said elastic segment respectively claim 1 , that ...

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Номер записи: 61
07-01-2016 дата публикации

METHOD FOR DIGITAL TRANSDUCTION OF DNA IN LIVING CELLS

Номер: US20160002620A1
Автор: Montagnier Luc
Принадлежит:

A system and method for inducing cytotoxicity, comprising a receiver configured to receive an electromagnetic signal from a container, using a receiver configured to capture electromagnetic emissions from the container over a frequency range of at least 100 Hz to 10,000 Hz; an amplifier configured to amplify the received electromagnetic signal; and an emitter configured to emit the amplified electromagnetic signal in proximity to living cells. DNA from a pathogen is amplified using PCR, purified, and serially diluted. Electromagnetic signals from the diluted DNA are received, and optionally stored. The receive signal is amplified and emitted in proximity to living cells, to produce under selected circumstances, a cytopathic effect. 1. A method of producing cytotoxicity , comprising:amplifying DNA from a pathogen using polymerase chain reaction technology;purifying the amplified DNA;serially diluting and mixing the purified DNA in water, to generate a dilute DNA sample in a container;receiving an electromagnetic signal from the container, using a receiver configured to capture electromagnetic emissions from the container over a frequency range of at least 100 Hz to 10,000 Hz;optionally recording the received electromagnetic signal;amplifying the received or optionally recorded electromagnetic signal; andemitting the amplified electromagnetic signal in proximity to living cells.2. The method according to claim 1 , wherein the purified DNA is constituted as 2 ng/ml in water claim 1 , and serially diluted over a range within 10to 10.3. The method according to claim 1 , wherein the received electromagnetic signal is digitally recorded for about 6 seconds over a bandwidth of at least 400 Hz to 4 kHz.4BorreliaRicketsiales.. The method according to claim 1 , wherein the pathogen is of a genus selective from the group consisting of and5. The method according to claim 1 , wherein serial dilutions of the purified DNA are analyzed for significant electromagnetic emissions by ...

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Номер записи: 62
07-01-2016 дата публикации

SOLAR STEAM PROCESSING OF BIOFUEL FEEDSTOCK AND SOLAR DISTILLATION OF BIOFUELS

Номер: US20160002673A1
Автор: Halas Nancy J., Neumann Oara, Nordlander Peter, Urban Alexander
Принадлежит: William Marsh Rice University

A method of producing bioethanol that includes receiving a feedstock solution that includes polysaccharides in a vessel comprising a complex is described. The complex may be copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and/or branched nanostructures. The method also includes applying electromagnetic (EM) radiation to the complex such that the complex absorbs the EM radiation to generate heat. Using the heat generated by the complex, sugar molecules may be extracted from the polysaccharides in the feedstock solution, and fermented. Then, bioethanol may be extracted from the vessel. 1. A method of producing bioethanol , the method comprising:receiving, in a vessel comprising a complex, a feedstock solution comprising polysaccharides, wherein the complex is a least one selected from a group consisting of copper nanoparticles, copper oxide nanoparticles, nanoshells, nanorods, carbon moieties, encapsulated nanoshells, encapsulated nanoparticles, and branched nanostructures;applying electromagnetic (EM) radiation to the complex, wherein the complex absorbs the EM radiation to generate heat;extracting, using the heat generated by the complex, sugar molecules from the polysaccharides in the feedstock solution; andfermenting the sugars molecules to generate bioethanol; andextracting the bioethanol from the vessel.2. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:condensing, using a condenser, the bioethanol from the vessel; andstoring the bioethanol in a storage tank.3. The method of claim 1 , wherein extracting the bioethanol from the vessel comprises:applying additional EM radiation to the complex, wherein the complex absorbs the additional EM radiation to generate additional heat;transforming, using the additional heat generated by the complex, the bioethanol to a vapor; andextracting the vapor from the vessel.4. The method of claim 1 , ...

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Номер записи: 63
04-01-2018 дата публикации

Apparatuses and methods for electroporation

Номер: US20180002652A1
Автор: John CESAREK, Richard Hansen, Travis Lee
Принадлежит: Individual

Disclosed are apparatuses, systems, and methods for performing electroporation.

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Номер записи: 64
07-01-2021 дата публикации

METHODS, SYSTEMS AND COMPOSITIONS FOR FUNCTIONAL IN VITRO CELLULAR MODELS OF MAMMALIAN SYSTEMS

Номер: US20210003554A1
Автор: Hickman James J.
Принадлежит:

The present invention comprises methods, systems and compositions comprising cell culture analog systems, comprising components which optionally comprise biologically functional cells, and the components and systems function similarly to in vivo conditions. 137-. (canceled)38. A method of assessing one or more effects of varying an input variable or a cell culture characteristic on a microfluidic cell culture analog system , the method comprising:varying an input variable or a cell culture characteristic to which a microfluidic cell culture analog system is exposed, the microfluidic cell culture analog system comprising one or more organ components, each organ component comprising one or more chambers, and each organ component (i) being microfluidically coupled to one another, and (ii) comprising cells cultured on a surface;recording, over a duration of time, changes in measured electrophysiological properties, contractile properties or both in response to the varying input variable or cell culture characteristic;in which the electrophysiological properties, if measured, are measured from a first population of cells cultured on a surface comprising a microelectrode array housed in a first chamber and the contractile properties, if measured, are measured from a second population of cells cultured on a surface comprising a cantilever array housed in a second chamber;the recorded changes providing an assessment of the one or more effects of varying an input variable or a cell culture characteristic.39. The method of claim 38 , wherein the duration of time is a time period of days.40. The method of claim 38 , wherein the multiple microfluidically coupled organ components include a cardiac component claim 38 , and the method further comprises measuring at least one electrophysiological property from cardiomyocyte cells cultured on a microelectrode array of the cardiac component and simultaneously measuring at least one contractile property from cardiomyocyte cells ...

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Номер записи: 65
07-01-2021 дата публикации

LOW SHEAR MICROFLUIDIC DEVICES AND METHODS OF USE AND MANUFACTURING THEREOF

Номер: US20210003561A1
Автор: Hajipouran Benam Kambez, Hamilton Geraldine A., Hassell Bryan, Hinojosa Christopher D., Ingber Donald E., Lucchesi Carolina, Villenave Remi
Принадлежит:

Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types. 1174-. (canceled)175. A method for culturing cells comprising: a. a body comprising a central channel therein; and', 'b. an at least partially porous membrane positioned within the central channel, the membrane configured to separate the central channel to format at least one microchannel and at least one mesochannel, wherein a height ratio of the at least one mesochannel to the at least one microchannel ranges from 10:1 to about 50:1;, '1) providing a microfluidic device comprising2) seeding human epithelial cells from a patient on said membrane facing the at least one mesochannel; and{'b': '2', '3) culturing the seeded human cells from step ) on said membrane submerged within a first liquid.'}176. The method of claim 175 , wherein the method further comprises:4) removing the first liquid from the at least one mesochannel, such that a gas-liquid interface is established, whereby said human cells are induced to differentiate into pseudostratified epithelial cells.177. The method of claim 175 , wherein the human epithelial ...

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Номер записи: 66
12-01-2017 дата публикации

Cell culture and experiment device

Номер: US20170009196A1
Автор: HAI Yuan, Jiandong Huang, Jinming CUI, Olaf Eichstaedt, Ruxu Du, Shijie ZENG
Принадлежит: Guangzhou Institute of Advanced Technology of CAS

The invention discloses a cell culture and experiment device used in the field of biological and genetic engineering experiment apparatus, comprising a central distribution compartment, a culture compartment, a treatment compartment, and pipelines for delivering liquid between the central distribution compartment and the culture compartment and between the central distribution compartment and the treatment compartment. The central distribution compartment is equipped with a distribution chamber and a piston which can be moved forward and backward in the distribution chamber to alter the working volume of the distribution chamber. At the bottom of the distribution chamber, the central distribution compartment is equipped with a distribution valve controlling the connectivity between the distribution chamber and any of the channels. The invention provides a miniaturized apparatus integrating the central distribution compartment, the culture compartment and the treatment compartment, which can replace manual operations, save time and labor, and avoid wasting experimental raw material.

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Номер записи: 67
12-01-2017 дата публикации

IN-VITRO BIOREACTOR

Номер: US20170009207A1
Автор: Schwartzentruber Jared, Shamir D. Barrett
Принадлежит: New York University

An in vitro chamber for mimicking the mechanical and electrical forces found in vivo under physiological or pathological state. A chamber provides conditioning stimuli. The conditioning stimuli replicate a pathology, including the ability to replicate a diseased pathology. The chamber is configured to allow the additional stimuli to test physical, chemical or electrical stimuli impact on cells experiencing the particular pathology. Pharmaceuticals may be tested ex-vivo on cells exhibiting a pathology in an environment mimicking the in-vitro environment. Physical components such as pacemakers may also be tested on such cells in the chamber. 1. An apparatus for simulating a heart comprising:a bioreactor having a media portion with a media chamber therein, and a lower control portion; the bioreactor having a cell layer for receiving cells; anda mechanical system and an electrical system in communication with the media layer, the mechanical system configured to exert physical stress upon the cells and the electrical system configured to exert electrical stimulation to the cells.2. The apparatus of wherein the electrical system includes electrodes conductively connected to the cell layer and configured for electrical stimulation of the cells.3. The apparatus of wherein the mechanical system includes a piston and a motor and wherein the cell layer comprises a membrane.4. The apparatus of wherein the membrane is positioned above the piston and adjacent the cell chamber when connected and movement of the piston causes fluid to flow through the cell membrane and cause sheet stress at the cells.5. The apparatus of claim 1 , further comprising a perfusion system in fluid communication with the bioreactor.6. The apparatus of claim 5 , wherein the lower control portion includes a control chamber and the mechanical system comprises a mechanical stretch apparatus and the cell layer includes a flexible dish.7. The apparatus of claim 6 , wherein the media portion comprises a media ...

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Номер записи: 68
27-01-2022 дата публикации

Electroporation With Active Compensation

Номер: US20220025402A1
Автор: CHANG CHIH-WEI, MEHRABI Artin, VAN LEW Jon Thomas
Принадлежит: NantCell, Inc.

An apparatus for electroporating cells with a cargo includes electrodes defining a path for a fluid including the cells and the cargo to flow, a power source coupled across the electrodes, and a control circuit. In some examples, the control circuit is configured to detect a decrease in an induced current due to an increase in a resistance between the electrodes, and control the power source to increase the induced current to maintain an electric field between the electrodes. A future value of the resistance between the electrodes may be predicted based on previous values of the resistance. In other examples, the control circuit is configured to detect parameters of the fluid flowing between the electrodes, and control the power source to generate or stop generating electrical pulses in response to detecting the parameters. Other example apparatuses, and methods of electroporating cells with a cargo is also disclosed. 1. An apparatus for electroporating cells with a cargo , the apparatus comprising:two electrodes spaced apart from each other and defining a path for a fluid including the cells and the cargo to flow therebetween, the electrodes having a resistance therebetween when fluid flows through the path;a DC power source coupled across the electrodes; anda control circuit configured to control the DC power source to provide a plurality of electrical pulses at a voltage to the electrodes to induce a current through the electrodes for generating an electric field between the electrodes at a defined value, detect a decrease in the induced current due to an increase in the resistance between the electrodes, determine a plurality of values of the resistance between the electrodes over a period of time, predict a future value of the resistance between the electrodes based on the determined plurality of values of the resistance, and control the DC power source to increase the induced current based on the future value of the resistance to maintain the electric field ...

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Номер записи: 69
11-01-2018 дата публикации

PLASMONIC NANOCAVITY-BASED CELL THERAPY METHOD AND SYSTEM

Номер: US20180010149A1
Автор: HUBER Marinus, Madrid Marinna, Mazur Eric, Saklayen Nabiha, VOGEL Nicolas
Принадлежит:

In one aspect, a structure for use in transfecting cells is disclosed, which comprises a matrix supporting a plurality of cavities, each cavity having an opening characterized by a rim and an inner surface subtending and/or extending from said rim. An electrically conductive coating is disposed on a top surface of the substrate between, and connecting, the rims of the cavities. A layer of an electrically conductive material can also coat at least a portion of each cavity's inner surface. At least one dimension of each cavity is in a range of about 50 nm to about 3.5 microns, e.g., in a range of about 100 nm to about 1 micron, or in a range of about 200 nm to about 800 nm, or in a range about 200 nm to about 500 nm. In some cases, all dimensions of the cavity (e.g., X, Y, an Z-Cartesian dimensions) are in the aforementioned ranges. 1. A plasmonic structure for use in transfecting cells , comprising:a matrix supporting a plurality of cavities, each cavity having an opening and an inner surface extending beneath said opening,a layer of an electrically conductive material coating at least a portion of said matrix ,wherein at least one dimension of each of said cavities is in a range of about 50 nm to about 2 microns.2. The plasmonic structure of claim 1 , wherein said electrically conductive material coats at least a portion of a top surface of said matrix.3. The plasmonic structure of claim 1 , wherein said electrically conductive material coats at least a portion of an inner surface of at least one of said cavities.4. The plasmonic structure of claim 1 , wherein said electrically conductive material coats at least a portion of a top surface of said matrix and at least a portion of an inner surface of at least one of said cavities.5. The plasmonic structure of claim 1 , wherein said electrically conductive coating coats an entire top surface of said matrix and an entire inner surface of each of said cavities.6. The plasmonic structure of claim 1 , wherein said at least ...

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Номер записи: 70
14-01-2021 дата публикации

MICROFLUIDIC CELLULAR MEMBRANE MODIFICATION DEVICES

Номер: US20210009931A1
Автор: Shkolnikov Viktor, XIN DAIXI
Принадлежит:

The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion. 1. A microfluidic cellular membrane modification device , comprising:a microfluidic channel including a pumping portion and an electric field portion;an electrode pair positioned about the electric field portion; anda bidirectional pump in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.2. The microfluidic device of claim 1 , wherein the bidirectional pump is integrated within the microfluidic channel.3. The microfluidic device of claim 1 , wherein the bidirectional pump comprises a thermal resistor to move fluid via thermally-generated bubbles.4. The microfluidic device of claim 3 , further comprising an ejection nozzle in fluid communication with the bidirectional pump to eject a portion of the fluid displaced by the thermally-generated bubbles.5. The microfluidic device of claim 1 , wherein the electric field portion includes a constriction having a constricted cross-sectional area relative to a cross-sectional area of the pumping portion.6. The microfluidic device of claim 5 , wherein the fluid to be loaded or loaded within the microfluidic channel includes cells claim 5 , and wherein the constricted cross-sectional area is large enough to accommodate a single cell carried by the fluid.7. The microfluidic device of claim 1 , wherein the microfluidic channel has a serpentine shape crossing the electrode pair multiple times to provide multiple electric field portions.8. The ...

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Номер записи: 71
10-01-2019 дата публикации

METHOD FOR PREDICTING RADIOSENSITIVITY OF A CELL

Номер: US20190010484A1
Автор: ILIAKIS George
Принадлежит: UNIVERSITAT DUISBURG-ESSEN

The present invention relates to a method for predicting the degree of radiosensitivity of a cell by determining the number of prompt double-strand breaks (prDSBs) and predicting the degree of radiosensitivity of the cell based on the number of prDSBs. The present invention relates furthermore to a method for predicting the degree of radiosensitivity of a cell by determining the number of thermally labile sugar lesion-dependent double-strand breaks (tlDSBs) and predicting the degree of radiosensitivity of the cell based on the number of tlDSBs. 1. An in vitro method for predicting the degree of radiosensitivity of a cell , comprising(a) irradiating a cell,(b) determining the number of prompt double-strand breaks (prDSBs) in the cell of step (a), and(c) using the number of prDSBs determined in step (b) to predict the degree of radiosensitivity of said cell, oran in vitro method for predicting the degree of radiosensitivity of a cell, comprising(a) irradiating a cell,(b) determining the number of thermally labile sugar lesion-dependent double-stand breaks (tlDSBs)) in the cell of step (a), and(c) using the number of tlDSBs determined in step (b) to predict the degree of radiosensitivity of said cell.2. (canceled)3. The method of claim 1 , wherein the number of tlDSBs is determined by subtracting the number of prDSBs from the number of total double-strand breaks (tDSBs).4. The method of claim 1 , wherein the cell is a diseased cell claim 1 , preferably a tumor cell claim 1 , more preferably of epithelial origin claim 1 , of mesenchymal origin claim 1 , of hematopoietic origin claim 1 , or of neuro-ectodermal origin claim 1 , still more preferably claim 1 , the cell is selected from a breast adenocarcinoma claim 1 , sweat gland adenocarcinoma claim 1 , salivary gland adenocarcinoma claim 1 , skin squamous cell carcinoma claim 1 , adenocarcinoma of the thyroid claim 1 , lung claim 1 , stomach claim 1 , liver claim 1 , pancreas claim 1 , small intestine claim 1 , colon ...

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Номер записи: 72
09-01-2020 дата публикации

Cellular Poration Using Laser Radiation

Номер: US20200010791A9
Автор: HUBER Marinus, Madrid Marinna, Mazur Eric, Nuzzo Valeria, Saklayen Nabiha
Принадлежит:

In one aspect, a method of cell processing is disclosed, which includes disposing a plurality of cells on a substrate across which a plurality of projections are distributed and an electrically conductive layer at least partially coating said projections, exposing the cells to a cargo to be internalized by the cells, irradiating the substrate surface (and in particular the projections) with continuous wave or pulsed laser radiation. For example, one or more laser pulses having a pulse width in a range of about 1 ns to about 1000 ns can be applied so as to facilitate uptake of the cargo by at least a portion of the cells (e.g., the cells positioned in the vicinity of the projections (e.g., within hundreds of nanometer (such as less than nm) of the projections)). In some embodiments, the laser pulses have a pulse width in a range of about 10 ns to about 500 ns, e.g., in a range of about 5 ns to about 50 ns. 153-. (canceled)54. A method of cell processing , comprising:disposing a plurality of cells on a substrate having a plurality of projections and an electrically conductive layer at least partially coating said projections,exposing the cells to a cargo to be internalized by the cells,irradiating the projections with one or more laser pulses having a pulse width in a range of about 1 ns to about 1000 ns so as to facilitate uptake of the cargo by at least a portion of said cells.55. The method of claim 54 , wherein said laser pulses have a pulse width in a range of about 10 ns to about 100 ns.56. The method of claim 54 , wherein said laser pulses have a pulse width in a range of about 20 ns to about 500 ns.57. The method of claim 54 , wherein the laser pulses are applied at a repetition rate of at least about 1 Hz.58. The method of claim 57 , wherein the laser pulses are applied to the cells at a repetition rate in a range of about 0.1 Hz to about 100 GHz.59. The method of claim 54 , wherein the laser pulses are applied with a fluence in a range of about 0.01 mJ/cmto ...

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Номер записи: 73
09-01-2020 дата публикации

SIMULTANEOUS SEPARATION AND ACTIVATION OF T CELLS FROM BLOOD PRODUCTS WITH SUBSEQUENT STIMULATION TO EXPAND T CELLS

Номер: US20200010826A1
Автор: Khristov Todor R., Liberti Paul A., RITTER Dustin W.
Принадлежит: Biomagnetic Solutions LLC

Embodiments disclosed herein relate to methods for purifying, activating, and expanding T cells, and subsets thereof. 2. The method of claim 1 , further comprising culturing the cells that are bound to the magnetic particles in the presence of a soluble co-stimulatory agent.3. The method of claim 2 , wherein the co-stimulatory agent is anti-CD28 claim 2 , B7-1 claim 2 , B7-2 claim 2 , anti-CD2 claim 2 , LFA-3 claim 2 , or any combination thereof.4. The method of claim 2 , wherein the at least one soluble co-stimulatory agent is mouse-derived anti-human CD28 of the IgG1 subclass.5. The method of claim 2 , wherein the at least one soluble co-stimulatory agent is biotinylated.6. The method of claim 5 , wherein the at least one soluble co-stimulatory agent is biotinylated anti-human CD28 claim 5 , or fragments thereof.7. The method of claim 2 , wherein the co-stimulatory agent is not bound to a particle.8. The method of claim 2 , wherein the amount of the at least one soluble co-stimulatory agent can be independently varied with respect to the level of the at least one labeling antibody.9. The method of claim 2 , wherein the soluble co-stimulatory agent is a mixture of a mouse-derived anti-human CD28 of the IgG1 subclass and a mouse-derived anti-human CD2 of the IgG1 subclass.10. The method of claim 2 , wherein the soluble co-stimulatory agent is added at a single time point after the separating step.11. The method of claim 2 , wherein the one soluble co-stimulatory agent is added immediately after the separating step.12. The method of claim 2 , wherein the co-stimulatory agent is added about 1 minute to about 20 hours after the separating step.1315-. (canceled)16. The method of claim 1 , wherein the labeled magnetic particles and the at least one antibody are mixed prior to being mixed with the blood product.17. The method of claim 1 , wherein the blood product is a whole peripheral blood product claim 1 , a leukapheresis product claim 1 , comprises mononuclear cells ...

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Номер записи: 74
21-01-2016 дата публикации

CRYOPRESERVATIVE COMPOSTIONS AND METHODS

Номер: US20160015025A1
Автор: BISCHOF John C., CHOI Jeunghwan, ETHERIDGE Michael L.
Принадлежит:

This disclosure describes compositions and methods related to cryoprotection of biomaterial. Generally, the cryoprotective composition includes a cryoprotective agent and magnetic nanoparticles effective for thawing a cryopreserved specimen comprising biomaterial with minimal biomaterial damage. In some embodiments, the composition is effective for thawing a cryopreserved specimen having a minimum dimension of 0.1 mm. Generally, the method includes obtaining a biomaterial cryopreserved with a cryoprotective composition as summarized above, then subjecting the cryopreserved biomaterial to electromagnetic energy of an intensity sufficient to excite the magnetic nanoparticles and thaw the biomaterial.

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Номер записи: 75
18-01-2018 дата публикации

Method for Virus Clearance

Номер: US20180015189A1
Автор: Castan Andreas, Tuohey Colin R.
Принадлежит:

The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution; ii) subjecting the bulk medium portion to a high temperature short time treatment (HTST); iii) passing the first additive portion through a virus retentive filter or an ultrafilter; and iv) after steps ii) and iii), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium. 1. A method for virus clearance of a cell culture medium , comprising the steps of:i) providing a bulk medium portion, comprising amino acids and glucose;ii) providing a first additive portion, comprising vitamins in aqueous solution;iii) subjecting the bulk medium portion to a high temperature short time treatment (HTST);iv) passing the first additive portion through a virus retentive filter or an ultrafilter; andv) after steps iii) and iv), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium.2. The method of claim 1 , wherein step iii) comprises heating the bulk medium portion to a temperature of about 85-110° C. claim 1 , maintaining said temperature for about 1 s to about 10 min claim 1 , and cooling the bulk medium portion to <40° C. claim 1 , such as 1 -40° C.3. The method of claim 2 , wherein in step iii) the rate of heating is at least 5° C. per s.4. The method of claim 2 , wherein in step iii) the rate of cooling is at least 5° C. per s.5. The method of claim 2 , wherein the heating is performed in a flow-through heater.6. The method of claim 5 , wherein the flow-through heater is an inductive heater.7. The method of claim 2 , wherein the cooling is performed in a flow-through cooler.8. The method of claim 1 , wherein the first additive portion comprises thiamine and/or pantothenic acid.9. The method of claim 1 , wherein the bulk medium portion is passed through a ...

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Номер записи: 76
18-01-2018 дата публикации

OPTOGENETIC CONTROL OF ENDOTHELIAL CELLS

Номер: US20180015296A1
Автор: BROWN Tyler C., MOORE Christopher I.
Принадлежит:

The invention features methods for regulating vascular properties by controlling the membrane properties of endothelial cells using optogenetics and light. The invention features methods to transport therapeutics across the vascular barrier into tissues such as the brain and the lung, with high spatial and temporal precision, and for controlling vascular properties such as vascular tone, arterial diameter, and vascular growth. 12-. (canceled)3. A method to deliver a therapeutic across the vascular endothelial barrier comprising endothelial cells expressing an optogenetic reagent in a subject , said method comprising the steps of: a) introducing said therapeutic into the blood stream of said subject; and b) contacting said endothelial cells with light , wherein said light activates said optogenetic reagent and thereby changes permeability of said endothelial cells and opens the vascular endothelial barrier such that said therapeutic in the blood stream crosses the vascular endothelial barrier.4. The method of claim 3 , wherein said optogenetic reagent is selected from the group consisting of ChR1 claim 3 , ChR2 claim 3 , VChR1 claim 3 , ChR2 C128A claim 3 , ChR2 C128S claim 3 , ChR2 C128T claim 3 , ChD claim 3 , ChEF claim 3 , ChF claim 3 , ChIEF claim 3 , NpHR claim 3 , eNpHR claim 3 , Arch 3.0 claim 3 , Arch T 3.0 claim 3 , Mac 3.0 claim 3 , melanopsin claim 3 , chimeras of these proteins and natural and engineered variants thereof.5. The method of claim 3 , wherein said optogenetic reagent are expressed in the endothelial cells by introducing a recombinant nucleic acid encoding the optogenetic reagent into said cells or precursors thereof.6. The method of claim 5 , wherein said recombinant nucleic acid is introduced into said cells by using any one or more of a virus claim 5 , an electroporation device claim 5 , a transfection method claim 5 , and a transgenic method.7. The method of claim 6 , wherein said recombinant nucleic acid is encapsidated within a ...

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Номер записи: 77
15-01-2015 дата публикации

Securing Apparatus and Method

Номер: US20150017719A1
Автор: Nickel Troy D., Owens Aaron M., Rausch Eugene Joseph, Zhang Guangtian
Принадлежит:

A securing apparatus includes a housing, a cam member, and a movable device that can be moved in a first direction to engage a first surface of the cam member to cause the cam member to move from a first position to a second position. When the cam member is in the first position, first and second movable members can be moved relative to the housing. When the cam member is in the second position, second and third surfaces of the cam member press respectively against the first and second movable members to trap the first and second movable members respectively between the second and third surfaces of the cam member and the housing such that the first and second movable members are fixed relative to the housing. 1. A securing apparatus , comprising:a housing;a cam member;a movable device that can be moved in a first direction to engage a first surface of the cam member to cause the cam member to move from a first position to a second position;a first movable member, wherein when the cam member is in the first position the first movable member can be moved relative to the housing, and wherein when the cam member is in the second position a second surface of the cam member presses against the first movable member to trap the first movable member between the second surface of the cam member and the housing such that the first movable member is fixed relative to the housing; anda second movable member, wherein when the cam member is in the first position the second movable member can be moved relative to the housing, and wherein when the cam member is in the second position a third surface of the cam member presses against the second movable member to trap the second movable member between the third surface of the cam member and the housing such that the second movable member is fixed relative to the housing.2. The apparatus of claim 1 , wherein the movable device is a screw.3. The apparatus of claim 1 , wherein the movable device can be moved in a second direction which ...

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Номер записи: 78
21-01-2016 дата публикации

DEVICE AND METHODS COMPRISING MICROELECTRODE ARRAYS FOR ELECTROCONDUCTIVE CELLS

Номер: US20160017268A1
Автор: Chae Junseok, GUPTA Kshitiz, KIM Deok-Ho, Laflamme Michael
Принадлежит:

The present invention relates to the devices and method comprising microelectrode arrays for the differentiation, maturation and functional analysis of electroconductive cells, including muscle cells (including, but not limited to, cardiomyocytes, skeletal muscle myocytes and smooth muscle myocytes) and neuronal cells. The microelectrode present on the arrays can be used to stimulate and record from cells cultured on the substrate. In some embodiments, the substrate has a substantially smooth surface, and in other embodiments the substrate is nanotextured, including an array of substantially parallel grooves and ridges of nanometer-micrometer widths.

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Номер записи: 79
18-01-2018 дата публикации

APPARATUS, SYSTEMS AND METHODS FOR PROGRAMMABLE TISSUE CULTURE ILLUMINATION

Номер: US20180016538A1
Автор: Bugaj Lukasz, Lim Wendell
Принадлежит:

The disclosed apparatus, systems and methods relate to an illumination opto-plate configured to specifically light the wells of a culture plate. 1. An illumination device , comprising: i. a plurality of LED fixtures;', 'ii. a microcontroller; and', 'iii. at least one LED driver; and, 'a. a base, comprising;'} i. at least one layer;', 'ii. a plurality of openings; and', 'iii. a plurality of walls,, 'b. an adaptor, comprising;'}wherein the isled fixtures are configured to illuminate a culture plate.2. The illumination device of claim 1 , wherein each of the plurality of LED fixtures is independently programmable.3. The illumination device of claim 1 , wherein the adaptor has three layers.4. The illumination device of claim 3 , wherein a plurality of diffuser paper is disposed between the adaptor layers.5. The illumination device of claim 3 , wherein the adaptor layers further comprise mounting points.6. The illumination device of claim 3 , wherein the adaptor further comprises a mounting ridge configured for reception of the culture plate.7. The illumination device of claim 6 , wherein the walls of the adaptor are configured for individual well illumination.8. The illumination device of claim 7 , wherein the opto-plate further comprises a memory.9. A system for illuminating a culture plate comprising: i. at least one LED fixture;', 'ii. at least one LED driver; and', 'iii. at least one microcontroller;, 'a. an opto-plate, comprising i. at least one layer;', 'ii. at least one opening; and', 'iii. a plurality of walls, wherein the adaptor is attached to the opto-plate such that when the culture plate is placed on the adaptor the LED fixtures illuminate the culture plate., 'b. an adaptor, comprising;'}10. The system of claim 9 , wherein the walls of the adaptor are configured such that each well within the culture plate is individually illuminated.11. The system of claim 9 , further comprising a lid.12. The system of claim 9 , further comprising an Arduino configured to ...

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Номер записи: 80
18-01-2018 дата публикации

DISRUPTION AND FIELD ENABLED DELIVERY OF COMPOUNDS AND COMPOSITIONS INTO CELLS

Номер: US20180016539A1
Автор: Ding Xiaoyun, Jensen Klavs F., Langer Robert S., Sharei Armon R.
Принадлежит:

A microfluidic system for causing perturbations in a cell membrane includes (a) a microfluidic channel defining a lumen and configured such that a cell suspended in a buffer can pass there through, and (b) source or emitter of an energy field. The microfluidic channel may include a cell-deforming constriction. A diameter of the constriction may be a function of the diameter of the cell. Related apparatus, systems, techniques, and articles are also described. 1. A microfluidic system for delivery of a payload to a cell , the system comprising: (a) an energy field; or', '(b) a source or emitter of an energy field positioned downstream, upstream, or upstream and downstream of said constriction., 'a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein the diameter of the constriction is a function of the diameter of the cell and is selected to induce temporary perturbations of the cell membrane large enough for the payload to pass through; and'}2. The microfluidic system of claim 1 , wherein the source or emitter of an energy field is positioned downstream of said constriction.3. The microfluidic system of claim 1 , wherein said energy field comprises:(a) an electrical field and said source emitter comprises an electrode;(b) a magnetic field and said source or emitter comprises a magnet or electromagnet;(c) an acoustic field and said source or emitter comprises a speaker; or(d) an optical field and said source or emitter comprises a light-emitting diode (LED), laser, or incandescent lightbulb.45.-. (canceled)6. The microfluidic system of claim 1 , wherein(a) the diameter of the constriction is selected to induce temporary perturbations of the cell membrane large enough for the payload to pass through, and the cell passes through the constriction to the field in a continuous flow, wherein after passing through said ...

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Номер записи: 81
18-01-2018 дата публикации

DEVICE FOR THE ELECTRICAL DISINTEGRATION OF CELL STRUCTURES, AND INSTALLATION AND USE OF THE DEVICE FOR PRODUCING FEED INTERMEDIATES AND FEED PRODUCTS

Номер: US20180016541A1
Автор: Fastenau Andreas, Hollah Clemens, Krampe Paul
Принадлежит:

Disclosed is a device for the electrical disintegration of cell structures including a chamber having an inlet for receiving material containing cell structures, an outlet for the discharge thereof, and a conveyor line extending between the inlet and the outlet, an electrode unit which has an electrode body which is arranged within the chamber at least portion-wise along the conveyor line, wherein the chamber has a wall which is portion-wise or completely electrically conductive and electrically insulated from the electrode body, and the electrode unit is adapted to generate an electric field between the electrode body and the wall for electrical disintegration. 1. A device for the electrical disintegration of cell structures , comprisinga chamber having an inlet for receiving material containing cell structures, an outlet for the discharge thereof, and a conveyor line extending between the inlet and the outlet,an electrode unit which has an electrode body which is arranged within the chamber at least portion-wise along the conveyor line, wherein:the chamber has a wall which is portion-wise or completely electrically conductive and electrically insulated from the electrode body,the electrode unit is adapted to generate an electric field between the electrode body and the wall for electrical disintegration, anda conveyor screw is arranged driveably in the conveyor line for conveying the material, wherein the electrode body is accommodated in a cavity in the conveyor screw.2. A device as set forth in claim 1 , wherein the conveyor screw has a hollow shaft and the electrode body is arranged coaxially with the conveyor screw in the hollow shaft.3. A device as set forth in claim 1 , wherein the conveyor screw partly or completely comprises electrically non-conductive material.4. A device as set forth in claim 3 , wherein an electrically non-conductive claim 3 , preferably organic claim 3 , polymer is used as the electrically non-conductive material.5. A device as set ...

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Номер записи: 82
19-01-2017 дата публикации

COMPOSITIONS AND METHODS FOR MEASURING CELLULAR MECHANICAL STRESS

Номер: US20170016885A1
Автор: CHEN-IZU Ye, IZU Leighton T., Lam Kit
Принадлежит: The Regents of the University of California

Provided are compositions and methods employing cells encapsulated within and attached to a hydrogel, e.g., for measuring mechanical strain and/or stress of the cell and for investigating the mechano-chemo-transduction mechanisms at cellular and molecular levels. 1. A composition comprising a cell encapsulated in a hydrogel , wherein the hydrogel is comprised of a first binding partner of a binding partner pair or a first binding partner of the binding partner pair conjugated to a biocompatible polymer , wherein the first binding partner is cross-linked with Y—X—Y crosslinker , wherein X is a linear hydrophilic polymer and Y is a second binding partner of the binding partner pair , and wherein the cell is attached directly or indirectly to the first binding partner.2. A composition comprising a cell encapsulated in a hydrogel , wherein the gel is comprised of first binding partner of a binding partner pair conjugated to a biocompatible polymer , wherein the biocompatible polymer is cross-linked with a boronate-X-boronate crosslinker , wherein X is a linear hydrophilic polymer , and wherein the cell is attached directly or indirectly to the first binding partner.3. The composition of claim 2 , wherein the boronate in the boronate-X-boronate crosslinker is selected from the group consisting of diboronate claim 2 , triboronate claim 2 , tetraboronate and mixtures thereof.4. The composition of any one of to claim 2 , wherein the binding partner pair is selected from the group consisting of avidin/biotin claim 2 , glutathione/glutathione S-transferases (GST) claim 2 , maltose-binding protein (MBP)/maltose claim 2 , Histag/Imidazole plus Zn2+ claim 2 , and vancomycin/D-alanine-D-alanine (DADA).5. The composition of claim 4 , wherein the avidin is selected from avidin claim 4 , streptavidin claim 4 , neutravidin and captavidin.6. The composition of claim 4 , wherein the vancomycin and the D-alanine-D-alanine (DADA) are monovalent claim 4 , divalent or trivalent.7. A ...

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Номер записи: 83
17-01-2019 дата публикации

SYSTEMS AND METHODS FOR CARDIOMYOCYTE PACING

Номер: US20190017028A1
Автор: Herron Todd, Jalife Jose, Jiang Jiang
Принадлежит:

Provided herein are systems and methods for cardiomyocyte pacing of cultured cells, including cardiomyoctyes. In particular, provided herein are systems and methods employing electrode arrays with multiwell culture devices that provide electrical stimulation to cells cultured in individual wells of the devices. 135-. (canceled)36. An electrode array that provides electrical current or voltage to a plurality of samples in a multiwell plate , the electrode array comprising a plurality of electrodes or electrode pairs , wherein each electrode or electrode pair provides a current or a voltage to a single well of a multiwell plate.37. The electrode array of wherein each electrode or electrode pair of the electrode array provides field stimulation to a sample in a well of the multiwell plate and/or provides point stimulation to a sample in a well of the multiwell plate.38. The electrode array of wherein each electrode or electrode pair of the electrode array is present in a low impedance circuit.39. The electrode array of wherein said well of the multiwell plate comprises a sample comprising a cell claim 36 , tissue claim 36 , organ claim 36 , or organoid.40. The electrode array of wherein said well of the multiwell plate comprises a sample comprising a material that responds to electrical stimulation.41. The electrode array of wherein said well of the multiwell plate comprises a sample comprising a cardiomyocyte.42. The electrode array of wherein the electrode array comprises 12 claim 36 , 24 claim 36 , or 96 electrodes or electrode pairs.43. A system for providing electrical stimulation to a plurality of samples claim 36 , the system comprising:a) an electrode array that provides electrical current or voltage to a plurality of samples in a multiwell plate, the electrode array comprising a plurality of electrodes or electrode pairs, wherein each electrode or electrode pair provides a current or a voltage to a single well of a multiwell plate; andb) a multiwell plate.44. ...

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Номер записи: 84
17-01-2019 дата публикации

RADIATION SENSOR

Номер: US20190017088A1
Автор: Yoon Chang, Ziaie Babak
Принадлежит:

A radiation sensor, comprising a housing, a first chamber disposed in the housing and configured to contain a microorganism. A second chamber is disposed in the housing and configured to contain a fermentation material, the second chamber separated from the first chamber by a breakable separator. A breaking member is configured to break the breakable separator when pressed by a user. A flexible membrane is configured to flex when the microorganism ferments and thereby releases a gaseous byproduct. An electronic indicator is configured to relay information indicating the amount of fermentation, when the radiation sensor has been exposed to radiation less fermentation takes place resulting in a smaller volume of released gaseous byproduct. 1. A radiation sensor , comprising:a housing;a first chamber disposed in the housing and configured to contain a microorganism;a second chamber disposed in the housing and configured to contain a fermentation material, the second chamber separated from the first chamber by a breakable separator;a breaking member configured to break the breakable separator when pressed by a user;a flexible membrane configured to flex when the microorganism ferments and thereby releases a gaseous byproduct;an electronic indicator configured to relay information indicating the amount of fermentation, when the radiation sensor has been exposed to radiation less fermentation takes place resulting in a smaller volume of released gaseous byproduct.2. The radiation sensor of claim 1 , the microorganism is yeast.3. The radiation sensor of claim 1 , the fermentation material is glucose.4. The radiation sensor of claim 1 , the gaseous byproduct is CO.5. The radiation sensor of claim 1 , the electronic indicator includes a proximity switch claim 1 , a battery and a light emitting diode (LED) claim 1 , such that sufficient release of the gaseous byproduct results in closing of the proximity switch and thereby coupling of the battery to the LED and thereby ...

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Номер записи: 85
16-01-2020 дата публикации

Methods for Culturing Cells in an Alternating Ionic Magnetic Resonance (AIMR) Multiple-Chambered Culture Apparatus

Номер: US20200017848A1
Автор: Goodwin Thomas J., Kushman Moses J.
Принадлежит: United States of America as Represented by the Administrator of the National Aeronautics and Space

Provided herein are systems and methods for culturing tissue-like assemblies of cells in the presence of a pulsating alternating ionic magnetic resonance field. The cells are introduced into a growth module in fluid connection with a nutrient module in which the gravity vector of the growth module is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field. 1. An alternating ionic magnetic resonance (AIMR) culture system for culturing a stable tissue-like assembly of cells , comprising: a first gas-permeable membrane disposed on a proximal end thereof;', 'a gas port fitted onto the first-gas permeable membrane; and', 'a first sealable opening on a distal end thereof; said distal end adaptable for an electrical connection;, 'a nutrient module, comprising a second gas-permeable membrane with a plurality of inlet/outlet ports thereon disposed on a proximal end thereof;', 'a baffling system on a distal end thereof; and', 'a semi-permeable membrane on the distal end thereof;, 'a growth module disposed within the nutrient module and fluidly connected with the first gas-permeable membrane thereof, comprisinga randomizing adapter comprising a randomizing mechanism in an electrical connection with the nutrient module received within an open proximal end thereof;a removable alternating ionic magnetic resonance chamber comprising an AIMR generating device with a diameter sufficient to receive at least the growth module therein.2. The AIMR culture system of claim 1 , wherein the nutrient module claim 1 , the growth module or a combination thereof are disposable.3. The AIMR culture system of claim 1 , wherein the nutrient module claim 1 , the growth module and the randomizing adapter are sterilizable.4. The AIMR culture system of claim 1 , wherein the AIMR generating device is configured to generate the pulsating alternating ionic magnetic resonance (AIMR) field with a frequency of about 7.8 Hz to about 59.9 Hz at fundamental ...

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Номер записи: 86
17-04-2014 дата публикации

ACTIVATION AND AGGREGATION OF HUMAN PLATELETS AND FORMATION OF PLATELET GELS BY NANOSECOND PULSED ELECTRIC FIELDS

Номер: US20140106430A1
Автор: BEEBE Stephen J., BLACKMORE Peter F., HARGRAVE Barbara Y., SCHOENBACH Karl H.
Принадлежит:

Methods for forming activated platelet gels using nsPEF's and applying the activated gels to wounds, such as heart tissue after myocardial infarction. The platelets are activated by applying at least one nsPEF with a duration between about 10 picoseconds to 1 microsecond and electrical field strengths between about 10 kV/cm and 350 kV/cm. 1. A method of treating injured heart tissue , comprising: activating the concentrated platelets by applying at least one electrical pulse to the concentrated platelets,', 'wherein the electrical pulse has a duration of at least about 100 picoseconds and less than about 1 microsecond and an electric field strength of at least about 10 kV/cm and less than about 350 kV/cm; and applying the activated platelets to heart tissue., 'concentrating platelets;'}2. The method of wherein the injured heart tissue is due to myocardial infarction.3. The method of claim 1 , further comprising:reperfusing the heart tissue.4. The method of claim 1 , wherein the platelets are autologous.5. The method of claim 1 , wherein the electrical pulse has a duration of 300 nanoseconds and the electrical field strength is 30 kV/cm. This application is a divisional of U.S. application Ser. No. 13/129,076, §371 filing date of Aug. 5, 2011, which claims priority to PCT/US2009/064431 filed on Nov. 13, 2009, which claims priority to U.S. Provisional Application No. 61/114,363, filed Nov. 13, 2008, which are incorporated by reference in their entirety.Electric fields can be used to manipulate cell function in a variety of ways. One specific cell mechanism that can be affected by electric fields is calcium mobilization within a cell. Calcium signaling, an important cell function, is responsible for a variety of cellular responses and actions. The release of internally stored calcium can stimulate responses to agonists, activate growth and respiration, cause the secretion of neurotransmitters, activate transcription mechanisms, cause the release of a variety of ...

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Номер записи: 87
28-01-2016 дата публикации

Non-conventional Cellular Based Immunotherapy

Номер: US20160024469A1
Автор: Wu Allan Yang
Принадлежит:

The proposed patent is a method of immunotherapy wherein unique and novel sources of antigen generating organisms/viruses/cells are incubated or electroporated into further novel immunogenic cells. This method may be used for cancer therapy or therapy of benign disease. This method may also be used for immunization from infectious organisms such as bacteria, fungi, viruses or parasites. An aliquot of cells or materials obtained from the antigen generating component or electroporated immunogenic cells may be preserved long-term (i.e. cryopreservation or lyophilization or desiccation) for the purpose of forming a library or archive for future therapy or drug development/research. 2. An aliquot of antigen generating cells or electroporated immunogenic cells may be preserved long-term (i.e. cryopreservation or lyophilization or desiccation) for the purpose of forming an archival library or source of future therapy or further research to develop new forms of therapeutic drugs. This invention claims benefit of priority to U.S. provisional patent application Ser. No. 61/958,486 filed Jul. 29, 2013.The invention relates generally to methods and systems for the delivery of manufactured therapeutic cells ex-vivo, which are eventually transferred in-vivo for the purpose of immunotherapy.Immunotherapy in the context of cellular therapy for cancer can be performed by transfecting expression vectors within dendritic cells to enhance the probability of antigen presentation and activate precise immune activity against any cell expressing the antigen such as a cancerous or unwanted cell or tissue. A good example of this is seen in the treatment of prostate cancer, in which vectors encoding modified prostate antigens may be transfected into dendritic cells (WO 2001074855 A3). This process, though efficacious, is time consuming and requires multiple complex steps in manufacturing. Furthermore, the process only takes advantage of the MHC II pathway of immunogenicity formation. This ...

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Номер записи: 88
28-01-2016 дата публикации

METHOD FOR TARGETTING GROWTH AND DEATH OF NEOPLASTIC CELLS BY BURSTS OF ENERGIES FROM CELLULAR ENERGY EMISSIONS

Номер: US20160025708A1
Автор: Manasheri Aria, Mirhoseini Mahmood, Mirhoseini Mary Cayton
Принадлежит:

The embodiments herein disclose a non-invasive method of using bursts of energies/electromagnetic field energies from cells to reduce or arrest the growth rate, proliferation of cancer cells/neoplastic cells. The energy from cells induces apoptosis in cancer cells, without harming normal cells beyond their physiologic threshold of survival are provided. The embodiments herein disclose a method for treatment of cancer/neoplastic cell in human or animals within the context of cancer therapeutics. A cell culture plate is incubated. This plate serves as the source of bursts energies/electromagnetic field energies. Further with a device the bursts of energies are targeted to another plate having cells for one week. After one week the microscopic examination is done. The rate of growth of cell is six to seven pulsatile cells per square centimetre. The energy from cells kills cancer cells, induce apoptosis, stimulate growth phase in cell culture and enables harmonics therapy. 1. A method for stimulating a cell culture for growth with bursts of energies/cellular signals of cells , the method comprises the steps of:culturing CD34 cells in three different culture plates comprising a culture medium by following a standard culture protocol;labelling a first culture plate as a control plate, and wherein the control plate comprises the CD 34 cells in a culture medium, and wherein the first culture plate is incubated at 37° C. for one week, and wherein the first culture plate is not exposed to any bursts of energies/cellular signals of cells;subjecting a second culture plate comprising CD 34 cells in a culture medium to a red light, and wherein the CD34 cells are stimulated by the red light constantly for one week, and wherein the second culture plate is incubated at 37° C. for one week;subjecting a third culture plate comprising CD 34 cells in a culture medium to a plurality of bursts of energies/cellular signals of the CD34 cells in the second culture plate with a device, and ...

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Номер записи: 89
10-02-2022 дата публикации

SELECTION METHOD OF iPS CELL, PREPARATION METHOD OF iPS CELL, AND CONTROL DEVICE

Номер: US20220041995A1
Автор: AKIYOSHI Ryutaro
Принадлежит: OLYMPUS CORPORATION

A selection method of an iPS cell includes: at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell; judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; and when the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process. 1. A selection method of an iPS cell , the selection method comprising:at a reprogramming process to culture a cell including a plurality of combinations of initializing factors labelled with luminescent genes that are different with each other, acquiring a photon number per unit area or a photon number per unit time of each of the luminescent genes of the cell;judging whether the acquired photon number is more than a threshold that is predetermined for the acquired photon number; andwhen the acquired photon number is more than the threshold, selecting this cell as an objective cell for a next process.2. The selection method of an iPS cell according to claim 1 , wherein in a captured image of the cell including the initializing factors claim 1 , the photon number is calculated by using brightness information of a prescribed area including a barycentric position of an external shape that forms a group of the cell.3. The selection method of an iPS cell according to claim 1 , wherein the judging includes classifying a probability to select a plurality of good iPS cells in a descendent order that is configured in accordance with the threshold.4. The selection method of an iPS cell according to claim 1 , wherein at the reprogramming process claim 1 , the cell including claim 1 , as the initializing factors claim 1 , a plurality of transcription factors selected from a transcription factor group ...

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Номер записи: 90
24-01-2019 дата публикации

BIO-REACTIVE SYSTEM AND METHOD FOR VOLTAGE CONTROLLED METABOLISM

Номер: US20190024035A1
Автор: YAU Siu-Tung
Принадлежит:

Bio-reactive systems for voltage controlled metabolism are described, that include electrochemical-electrostatic systems having a conventional three electrode cell modified with at least one additional gating electrode. The rate of a metabolic process occurring in at least one organism disposed on a working electrode is controllable by applying a gating voltage VG to the at least one gating electrode. A method for voltage controlled metabolism in a bio-reactive electrostatic cell that includes applying a gating voltage VG to at least one gating electrode is also described. The rate of a metabolic process may be controlled by altering at least one of the magnitude and polarity of the applied gating voltage VG. The method for voltage controlled metabolism may further be used to treat cancer and/or increase the rate of ethanol production by fermentation. 18-. (canceled)9. A method for voltage controlled metabolism in an electrostatic bio-reactive cell , comprising:suspending at least one organism and at least one metabolic substrate that can be metabolized by the organism in a solution in contact with a first electrode and at least one second electrode, wherein one or both electrode is coated with an insulator so that no current flows in the circuit;{'sub': 'G', 'applying a gating voltage Vbetween the first electrode and at least one second electrode disposed within the solution; and'}{'sub': 'G', 'controlling a rate of a metabolic reaction caused by the at least one organism by selecting at least one of the magnitude and polarity of the applied gating voltage V.'}10. The method of claim 9 , wherein the organism is yeast.11. The method of claim 9 , wherein the organism is algae.12. The method of claim 9 , wherein the metabolic substrate is a sugar.1314-. (canceled)15. The method according to claim 9 , wherein the gating voltage Vchanges the rate of the metabolic reaction.16. (canceled)17. The method of claim 9 , wherein the metabolic substrate is metabolized to ethanol ...

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Номер записи: 91
24-01-2019 дата публикации

APPARATUSES AND METHODS USING NANOSTRAWS TO DELIVER BIOLOGICALLY RELEVANT CARGO INTO NON-ADHERENT CELLS

Номер: US20190024122A1
Автор: CAO Yuhong, HJORT Karl Martin, LEAL-ORTIZ Sergio, Melosh Nicholas A., REHSE Chris, TAY Andy Kah Ping
Принадлежит:

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications. 1. A method of delivering a biologically relevant cargo into non-adherent cells , the method comprising:applying a force to drive a suspension of cells into contact with a plurality of nanostraws, wherein the plurality of nanostraws extend through a substrate and a distance beyond the substrate that is between 2 nm and 50 μm, further wherein the plurality of nanostraws are hollow and have an inner diameter of between 5 nm-1500 nm; anddriving the cargo from the nanostraws into an intracellular volume of the cells so that at least 20% of the cells take up the biologically relevant cargo.2. The method of claim 1 , wherein applying the force comprises moving the nanostraws to contact the cells.3. The method of claim 1 , wherein applying the force comprises centrifuging the suspension of cells to drive the cells into contact with the plurality of nanostraws.4. The method of claim 1 , further comprising incorporating magnetic particles on or in the cells claim 1 , and wherein applying force comprises applying a magnetic field to drive the suspension of cells into contact with the plurality of nanostraws.5. The method of claim 1 , wherein driving the cargo comprises applying a pulsed electrical field.6. The method of claim 1 , wherein the cargo comprises one or more of: nucleic acids or proteins.7. The method of claim 1 , further comprising separating the cells from the nanostraws.8. The method ...

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Номер записи: 92
24-04-2014 дата публикации

Apparatus and Method for Culturing Cells and Tissue

Номер: US20140113345A1
Автор: BARBER Katherine L., LEE Peter G.W., TILLOTSON Douglas L., UDALE Richard T.
Принадлежит:

An apparatus that provides synchronized chronic electrical stimulation and chronic stretch to a cell culture or tissue. The apparatus includes a programmable controller which synchronizes stretch and electrical stimulation applied to a deformable culture container, such as a silicone rubber culture dish. The apparatus is used to treat cells and retard de-differentiation or promote differentiation and maturation in some applications. 2. The apparatus according to claim 1 , wherein the apparatus comprises a plurality of deformable cell culture containers having cavities for receiving cells or tissue claim 1 , each cavity being provided with at least 2 electrodes disposed therein and each deformable container being coupled to means for providing chronic stretch to said cell culture containers and wherein the programmable controller has one or more electrical stimulation output(s) coupled to the electrodes disposed in each culture container adapted to provide chronic electrical stimulation in the form of electrical stimulation pulses suitable for stimulating cells and one or more stretch outputs coupled to the means for providing stretch to said containers and thus provides a stretch control signal thereto claim 1 , and wherein further the controller is adapted to synchronize the chronic electrical stimulation provided to the electrodes and the chronic stretch signals provided to the means for providing chronic stretch to the deformable cell culture containers.3. The apparatus according to claim 2 , wherein the means for providing chronic stretch to the plurality of cell culture containers comprises a fixed support and a movable support upon both of which the plurality of cell culture containers are mounted.4. The apparatus according to claim 3 , wherein the electrodes of the cells are mounted on a removable printed circuit board attached to said fixed support.5. The apparatus according to claim 4 , wherein the fixed support has a pair of contacts communicating with the ...

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Номер записи: 93
02-02-2017 дата публикации

METHOD FOR PREPARING ARTIFICIAL BLOOD VESSELS

Номер: US20170029781A1
Автор: OU KENG-LIANG
Принадлежит:

A method for preparing artificial vessels comprises preparing a template by 3D printing; preparing an active polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (active POSS-PCU); mixing the active POSS-PCU with stem cells to form the artificial vessels by 3D printing followed by plasma processing; removing the template to form the artificial blood vessels with an access. The method provides to prepare the artificial vessels with three-layer structures, which are capable of transporting nutrients and oxygen, removing metabolic wastes and enhancing haemocompatibility and biological stability. Therefore, the method for preparing the artificial vessels solves the problem of angiemphraxis caused by the artificial vessels with single-layer structure. 1. A method for preparing artificial blood vessels comprising steps of:preparing a template by 3D printing;preparing an active polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (active POSS-PCU);preparing stem cells;{'sup': 6', '6, 'mixing the active POSS-PCU with the 1×10to 2×10stem cells to form a mixed material containing the stem cells at 4° C.; enveloping the template with the mixed material, and sequentially forming the artificial blood vessels with three layers in a sequential order away from the template; and'}processing the artificial blood vessels with three layers by plasma processing, and removing the template to form the artificial blood vessels with an access.2. The method as claimed in claim 1 , wherein the source of the stem cells comprises bone marrow or peripheral blood.3. The method as claimed in claim 2 , wherein the stem cells express surface antigen CD133 or CD34.4. The method as claimed in claim 1 , wherein in the step of mixing the active POSS-PCU with the stem cells claim 1 , the amount of the stem cells is 1×10.5. The method as claimed in claim 1 , wherein in the step of processing the artificial blood vessels with three layers by plasma processing claim 1 , ...

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Номер записи: 94
02-02-2017 дата публикации

RADIOFREQUENCY DEVICE FOR INCREASING INTRACELLULAR BIOACTIVE SUBSTANCE AND PLANT CELL CULTURE METHOD USING THE SAME

Номер: US20170029803A1
Автор: CHO Moon-Jin, HEO Mun-Seong, JUNG Hae-Soo, KANG Hyo-Seok, KIM Eun-Ae, LEE Hyun-E, Lee Jeong-Hun, LEE Jin-Hyeong, LEE Seung-Jun, Lee Yu-Ri, MOH Ji-Hong, MOH Sang-Hyun, OH Hyun-Jin, SEO Hyo-Hyun, SHIN Dong-Sun, YOO Ser-Jong, YU Yeong-Wook
Принадлежит:

There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites. 1. A cell culture method for increasing amount of an intracellular bioactive substance , the method comprising:(a) a culturing step of inoculating a plant cell into a medium and culturing the plant cell; and(b) a radiofrequency processing step of processing the cultured cell with a radiofrequency for increasing amount of an intracellular bioactive substance in the plant cell.2. The cell culture method of claim 1 , wherein the radiofrequency is used for process in a range of 0.1 to 15 MHz.3. The cell culture method of claim 2 , wherein the culturing step further includes:(a1) a callus inducing step of culturing the plant cell in an MS(Murashige and Skoog) medium containing IAA (indole acetic acid), BAP (6-benzylaminopurine), sucrose, and gelite for 2 to 5 weeks for inducing a callus from a germinated plant; and(a2) an adventitious root inducing step of culturing the callus in an MS medium containing IBA (indole-3-butyric acid), MES monohydrate(2-(N-morpholino), benzyladenine, sucrose, and gelite for 2 to 5 weeks for inducing an adventitious root from the callus.4. The cell culture method of claim 3 , wherein the ...

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Номер записи: 95
04-02-2016 дата публикации

METHODS AND DEVICES FOR MECHANICAL AND ELECTRICAL STIMULATION OF STEM CELL MONOLAYER AND 3D CULTURES FOR TISSUE ENGINEERING APPLICATIONS

Номер: US20160032234A1
Автор: BAYES GENIS Antonio, BRAGÓS BARDIA Ramón, LLUCIÀ VALLDEPERAS Aida, ROSELL FERRER Francesc Xavier, SÁNCHEZ TERRONES Benjamin
Принадлежит:

Devices and methods for stimulating mechanically or electromechanically stem cell monolayer and 3D cultures for tissue engineering applications are disclosed. A bracket is proposed to be used in a disposable stimulation device. A disposable stimulation device is also proposed for accommodating a cell culture in a flexible area. Finally, an apparatus is proposed to mechanically extend the disposable stimulation device to induce mechanical stimulation. Additionally, a pair of electrodes placed at two opposing sides of the flexible area creates an electric field to induce electrical stimulation. Accordingly, a method to stimulate electromechanically a cell culture is proposed. 1. A bracket attachable to a flexible cell culture pool to form a stimulation device , the bracket comprising:a first portion adapted to accommodate a ferromagnetic element, wherein a first side of the first portion of the bracket is attachable to a side of the flexible cell culture pool.2. The bracket according to claim 1 , wherein one or both:the bracket further comprises a ferromagnetic element embedded in the first portion, and,wherein one or both the ferromagnetic element and the embedded ferromagnetic element is a magnet.3. (canceled)4. The bracket according to claim 1 , further comprising a second portion adjacent to a second side of the first portion claim 1 , wherein the second side shares an edge with the first side claim 1 , and wherein the area of the second portion extends beyond the area of the second side of the first portion.5. The bracket according to claim 4 , wherein one or more of:the second portion is attached to the first portion;the second portion forms one body with the first portion;the second portion is made of biocompatible material; and,the second portion is made of biocompatible material wherein the biocompatible material is polydimethylsiloxane (PDMS).6. (canceled)7. (canceled)8. The bracket according to any of claim 1 , wherein one or both:the first portion is made ...

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Номер записи: 96
17-02-2022 дата публикации

COUPLED SORTING AND ELECTRIC TREATMENT OF BIOLOGICAL CELLS

Номер: US20220049208A1
Автор: PEHAM Johannes, WASSERMANN Klemens, WIMBERGER Terje
Принадлежит:

The present application provides a method for lysis or electroporation of cells in a biological sample including the following steps: 1. A method for lysis or electroporation of cells in a biological sample comprising the following steps:passing cells of the sample, preferably suspended in a fluid, through a flow path with a flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path comprises at least two electrodes for generating an electric field, which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coating at least covers the surface of the electrodes that faces the flow path; andwhen the presence of a specific cell is detected in the detection apparatus, then an electric field is generated between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the electric field causes electroporation or lysis of the cell.2. The method according to claim 1 , wherein the strength of the electric field is in the range of 500 V/cm to 50 kV/cm.3. The method according to claim 1 , wherein the potential difference between the electrodes for generating the electric field is in the range of 1 V to 100V.4. The method according to claim 1 , wherein the biological sample comprises cells of a first group and cells of a second group claim 1 , wherein cells of the first group are exposed to a first electric field with a first electric field strength and cells of the second group are exposed to a second electric field with a second electric field strength.5. The method according to claim 4 , wherein the first electric field causes lysis of the cells of the first group and the second electric field causes reversible electroporation of the cells of the second group.6. The method according to claim 1 , wherein the biological sample ...

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Номер записи: 97
17-02-2022 дата публикации

SPECIFIC ELECTROPORATION AND LYSIS OF EUKARYOTIC CELLS

Номер: US20220049239A1
Автор: PEHAM Johannes, WASSERMANN Klemens, WIMBERGER Terje
Принадлежит:

A method of targeted electroporation and/or lysis of eukaryotic cellular bodies in a biological sample with at least two subgroups of eukaryotic cellular bodies, wherein each subgroup has a different susceptibility to electroporation and/or lysis in electric fields, including the following steps: transferring the biological sample in a chamber, exposing the biological sample to an electric field in the chamber, wherein the electric field is generated by at least two electrodes which are coated with a dielectric material with a relative permittivity greater than 3.9, and selecting the electric parameters of the electric field such as the field strength, the frequency or the wave form so that the subgroups are differently affected by said electric field for electroporation and/or lysis; as well as devices for the method. 1. A method of targeted electroporation and/or lysis of eukaryotic cellular bodies in a biological sample with at least two subgroups of eukaryotic cellular bodies , wherein each subgroup has a different susceptibility to electroporation and/or lysis in electric fields , comprising the following steps:transferring the biological sample in a chamber;exposing the biological sample to an electric field in the chamber, wherein the electric field is generated by at least two electrodes which are coated with a dielectric material with a relative permittivity greater than 3.9; andchoosing the electric parameters of the electric field such as the field strength, the frequency or the wave form so that the subgroups are differently affected by said electric field for electroporation and/or lysis.2. The method according to claim 1 , wherein the dielectric material has a thickness below 1 μm.3. The method according to claim 1 , wherein the distance between the electrodes is below 1 mm claim 1 , but greater than 5 μm claim 1 , and/or the electric field strength is in the range of 500 V/cm to 50 kV/cm.4. The method according to claim 1 , wherein the potential ...

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Номер записи: 98
04-02-2021 дата публикации

Microbe inactivation processing method and cell activation processing method

Номер: US20210030906A1
Автор: Tatsushi Igarashi
Принадлежит: Ushio Denki KK

Disclosed are a microbe inactivation processing method that can perform inactivation processing of microbes, while damage to human body cells is prevented or inhibited, with an efficient use of light emitted from a light source and the obtainment of a large effective irradiation area. Also provided are a cell activation processing method that can reliably activate target cells with high efficiency. The microbe inactivation processing method includes: a step of applying light emitted from a light source through an optical filter, with the light source configured to emit light having a wavelength within a wavelength range of 190 nm to 237 nm, in order to perform inactivation processing of a target microbe. When the light emitted from the light source is incident at an incident angle of 0°, the optical filter transmits at least a part of ultraviolet light having a wavelength within a range of not lower than 190 nm and not more than 230 nm, and transmits at least a part of ultraviolet light having a wavelength within a range of more than 230 nm and not more than 237 nm, and the optical filter blocks transmission of ultraviolet light having a wavelength out of a wavelength range of not lower than 190 nm and not more than 237 nm.

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Номер записи: 99
31-01-2019 дата публикации

THREE-DIMENSIONAL ACOUSTIC MANIPULATION OF CELLS

Номер: US20190031999A1
Автор: Dao Ming, Guo Feng, Huang Tony Jun, Mao Zhangming, Suresh Subra
Принадлежит:

Methods and devices for manipulating one or more particles (e.g., cells) in three dimensions using surface acoustic waves is described. Methods and devices for printing or more biological cells onto a substrate using surface acoustic waves are also provided. 1. A method of manipulating one or more particles in a reservoir in three dimensions , wherein the reservoir is disposed on a substrate , comprising:generating a first surface acoustic wave (SAW) and a second SAW along a first axis of the substrate, wherein the first SAW and the second SAW are generated from opposite sides of the reservoir;generating a third SAW and a fourth SAW along a second axis of the substrate, wherein the third SAW and the fourth SAW are generated from opposite sides of the reservoir, and wherein the first axis and the second axis intersect in the reservoir;manipulating the one or more particles in the reservoir to move along the first axis and/or the second axis by varying a frequency and/or a phase of at least one of the first, the second, the third, and/or the fourth SAW; andmanipulating the one or more particles in the reservoir to move along an axis that is orthogonal to the first axis and the second axis by varying an acoustic power of at least one of the first, the second, the third, and/or the fourth SAW.23-. (canceled)4. The method of claim 1 , wherein the first axis is at an angle ranging from 1-90 degrees relative to the second axis.56-. (canceled)7. The method of claim 1 , wherein the first acoustic wave claim 1 , the second acoustic wave claim 1 , the third acoustic wave claim 1 , and the fourth acoustic wave are generated by a first claim 1 , a second claim 1 , a third claim 1 , and a fourth claim 1 , interdigital transducer (IDT).8. The method of claim 1 , wherein the first acoustic wave claim 1 , the second acoustic wave claim 1 , the third acoustic wave claim 1 , and the fourth acoustic wave are generated by a first claim 1 , a second claim 1 , a third claim 1 , and a ...

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Номер записи: 100