28-01-2021 дата публикации
Номер: US20210024910A1
Принадлежит:
NOVOZYMES A/S
The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. 1. An isolated mannanase variant , or recombinant polypeptide or an active fragment thereof , wherein comprising a modification at one or more positions corresponding to a position selected from the positions 8 , 9 , 11 , 13 , 18 , 21 , 34 , 37 , 45 , 47 , 65 , 100 , 101 , 104 , 107 , 108 , 110 , 114 , 115 , 116 , 132 , 133 , 142 , 147 , 152 , 154 , 164 , 169 , 173 , 174 , 176 , 177 , 180 , 183 , 185 , 196 , 199 , 201 , 202 , 205 , 206 , 210 , 215 , 226 , 229 , 231 , 239 , 243 , 245 , 257 , 260 , 267 , 270 , 275 , 278 , 282 , 283 , 284 , 288 , 292 , 293 , and 295 of the polypeptide of SEQ ID NO: 2 , wherein each modification is independently a substitution , insertion , or deletion , wherein said variant has at least 59% but less than 100% sequence identity to the polypeptide of SEQ ID NO: 1 , the polypeptide of SEQ ID NO: 2 , the polypeptide of SEQ ID NO: 3 , or the polypeptide of SEQ ID NO: 4 , and wherein said variant , polypeptide or fragment has mannanase activity.2. (canceled)3. (canceled)4. (canceled)5. The variant claim 1 , polypeptide claim 1 , or fragment thereof according to claim 1 , wherein said variant claim 1 , polypeptide claim 1 , or fragment thereof has an improved detergent stability claim 1 , improved protease stability and/or improved thermostability.6. The variant claim 1 , polypeptide claim 1 , or fragment thereof according to claim 1 , wherein said variant claim 1 , polypeptide claim 1 , or fragment thereof comprises a substitution or deletion at one position corresponding to positions 8 claim 1 , 9 claim 1 , 11 claim 1 , 13 claim 1 , 18 claim 1 , 21 claim 1 , 34 claim 1 , 37 claim 1 , 45 claim 1 , 47 claim 1 , 65 claim 1 , 100 claim 1 , 101 claim 1 , 104 claim 1 , 107 claim 1 , 108 claim 1 , 110 ...
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