Selectable/reporter gene for use during genetic engineering of plants and plant cells
Номер патента: CA2134261C
Опубликовано: 06-10-1998
Автор(ы): Lawrence E. Pelcher
Принадлежит: Individual
Опубликовано: 06-10-1998
Автор(ы): Lawrence E. Pelcher
Принадлежит: Individual
Реферат: Adenosine deaminase gene (ADA gene) is used as a selectable/reporter gene for plant cells and plants. When introduced into plant cells, the ADA gene makes the cells resistant to inhibition by 2-deoxyadenosine or an analog, so transformed cells can be separated from non-transformed cells by culturing a cell mixture in a medium containing 2-deoxyadenosine or an analog in amounts that inhibit normal plant cells. A single vector may be constructed containing an ADA gene from any source (e.g. a mouse ADA gene) and an additional gene-of-interest to be introduced into plant cells. The vector may then be introduced into the cells by such a route as Agrobacterium infection or micro projectile bombardment. The presence of the ADA gene in successfully transformed plant cells may be confirmed by cultivating the cells in a medium containing 2-deoxyadenosine or an analog in the presence of a material that changes color in the presence of ammonia. Observation of a color change confirms the presence of the gene. DNA constructs containing both ADA genes and foreign genes-of-interest may be constructed, e.g. plasmid pRD360-ADA (ATCC 69459).
MODIFIED BACTERIAL NITRILLASE, BACTERIAL HOST, PLASMIDE, DNA SEQUENCE, VEGERAL CELL, PART OF PLANT, PLANT AND METHOD TO PRODUCE A SPECIFIC NITRILASE CAPABLE OF HYDROLYZING P-HYDROXYBENZONITRILA
Номер патента: BR8807609A. Автор: David Stalker. Владелец: Rhodia Agro Sa. Дата публикации: 1990-05-29.