Method for establishing an enteritis cell model and an application thereof

Реферат: The invention discloses a method for establishing an enteritis cell model and an application thereof, and belongs to the field of biotechnology. The invention is realized through three steps of cell culture inoculation, arachidonic acid modeling concentration screening and enteritis model establishment. The inducer of the in vitro enteritis cell model provided by the invention is arachidonic acid, and a new in vitro enteritis cell model is established by taking the characteristic secondary metabolites PGE2, LTB4 and cytotoxicity assay as cell model evaluation methods, and continuously intervening NCM-460 cells with 40 μg/mL arachidonic acid for 24 hours. The invention provides a new in vitro model for the rapid screening of new therapeutic drugs for enteritis, the rapid screening of anti-inflammatory drugs and the study of drug action mechanism.