УСТРОЙСТВО ДЛЯ МОЛЕКУЛЯРНОГО ОБМЕНА

Группа изобретений относится к устройствам молекулярного обмена. Устройство содержит корпус, имеющий две внутренние полости с проксимальным и дистальным концами, разграниченные разделителем. Разделитель расположен вдоль центральной оси корпуса. Полости пролегают от проксимального конца к дистальному концу корпуса, и в них располагаются проводящие каналы для жидкостей на соосных сторонах разделителя. В корпусе выполнены обменные апертуры. По меньшей мере, часть каждого из проводящих каналов, находящихся в области обменной апертуры корпуса, выполнена пористой. Разделитель установлен вдоль корпуса, по меньшей мере, на длину обменной апертуры. Система для контроля концентраций первого вещества в проводящем канале для жидкостей устройства молекулярного обмена включает в себя устройство молекулярного обмена и контролирующее устройство, подсоединенное к устройству молекулярного обмена. Контролирующее устройство измеряет концентрации второго вещества в проводящих каналах для жидкостей и контролирует ...

Filter for preparing fluid for measuring contents for online measuring device comprises housing and filter insert with peripheral seal

A filter comprises a housing (1) and a filter insert (3) with a peripheral seal (2).

Salvia collection, processing, stabilization, and storage method

Salvia collection, processing, stabilization, and storage method

PROCEDURE AND DEVICE FOR DIFFUSION EXCHANGE BETWEEN NOT MIXABLE LIQUIDS

FILTER WITH INTEGRATED CONTROL OF THE SAMPLE VOLUME

Identification of host cell proteins

Methods of identifying host-cell proteins in a sample matrix are provided. The methods comprise: dissociating the host-cell protein from the protein of interest; filtering the dissociated host-cell protein using a molecular weight cut-off filter; and identifying the host-cell protein, preferably with mass spectrometry.

Use of hollow fibers to obtain blood or a blood derivative impoverished from blood cells and platelets derived extracellular vesicles

The present invention relates to the use of hollow fibers having a porosity above 20 nm, in particular polyethersulfone hollow fibers, to impoverish blood and blood-derivatives from blood-derived extracellular vesicles, in particular exosomes and exomers, and to methods for obtaining and analysing such impoverished samples.

Method and apparatus for diffusive transfer between immiscible fluids

FILTRATION ASSEMBLY

A filtration assembly (10) for use in collecting a fluid sample possibly containing microorganisms, and capturing the microorganisms of interest in the sample such that the captured microorganisms can be subsequently detected. The filtration assembly includes a sample reservoir (20) for collecting and holding the fluid sample to be filtered, a fluid port (38) and a vent (70) in communication with the sample reservoir, a filter element (45) disposed in a flow path between the sample reservoir and fluid port, and a cover member (50) detachably covering the sample reservoir.

WATER SEPARATION FROM SOLVENT

An apparatus (100) and method for separating residual water from a solvent. The device comprises a reservoir (102) containing a solution comprising solvent containing residual water, the reservoir having an opening to allow the solution to drain from the reservoir. A membrane layer is provided comprising a first layer (104) of fluoropolymer and a second layer of fluoropolymer (105). The membrane is positioned in series with the reservoir opening. Vacuum is generated on one side of the membrane layer wherein the solvent containing water passes through the membrane therein removing water from the solvent to provide a solvent with a water level of less than or equal to 1.0 ppm.

DEVICES AND PROCESSES FOR COLLECTING AND CONCENTRATING SAMPLES FOR MICROBIOLOGICAL ANALYSIS

The present invention refers to manual devices for the collecting and con centrating liquid samples for microbiological analysis and their respective methods of use. The manual devices comprise a body which contains a sample, a removable support, a microporous membrane, and a plunger. The present inve ntion is also directed to methods of collecting and concentrating of liquid samples onto a microporous membrane for microbiological analysis.

DIALYSIS DEVICE WITH AIR CHAMBER

ASSAYS TO DETECT NEURODEGENERATION

Methods of measuring the amount of singly- or multiply-phosphorylated p217+ tau protein in a sample are provided. Methods of detecting or diagnosing tauopathies, methods of determining the effectiveness of a treatment of a tauopathy, and methods of determining whether a subject is suitable for anti-p217+ tau antibody therapy are also provided. Also described are antibodies for use in the methods and kits comprising the antibodies.

SYSTEMS AND METHODS FOR QUANTIFYING AND MODIFYING PROTEIN VISCOSITY

Systems and methods for determining regions of proteins that contribute to the viscosity of formulations of those proteins are provided. Methods for modifying the viscosity of concentrated protein formulations are also provided.

METHODS AND APPARATUS TO SELECTIVELY EXTRACT CONSTITUENTS FROM BIOLOGICAL SAMPLES

Methods and apparatus provide filtration for concentrating analytes, such as bacteria or exosomes, of a biological sample, such as blood or urine. The technology may employ membrane devices that implement one or more tangential flow filtration processes such as in stages. An example membrane device may typically include a membrane having sides and ends. The membrane may selectively permit constituent(s) of the sample to pass through while retaining other constituents at one side. An input chamber of the device may include an inlet near one end and an outlet near the other end, and that may permit a tangential flow of the sample along the first side surface, and a trans-membrane passing of constituent(s). An output chamber of the device may be configured at the second side surface to receive the passing constituents. Such devices may be provided in a kit to facilitate targeting of a desired biological analyte concentration.

DEVICES, SYSTEMS, AND METHODS FOR REMOVAL OF SOLUBLE GASES FROM FLUID SAMPLES

Devices, systems and methods are disclosed which relate to using containers with a multitude of nucleation sites covering a major portion of the inside wall of the container to enable rapid and nearly complete removal of soluble gases from fluid samples, including carbonated beverages and other carbonated fluid samples. A fluid sample is rapidly poured into the described container initiating a catastrophic release of the soluble gas from the sample.

BIOLOGICAL FLUID SAMPLING TRANSFER DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid sampling transfer device that is adapted to receive and separate a multi-component blood sample is disclosed. After separation, the biological fluid sampling transfer device is able to transfer a plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device of the present disclosure also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

BLOOD SAMPLING TRANSFER DEVICE

A blood sampling transfer device that includes a lancing tape having a flow channel and a transfer cartridge removably connected to the lancing tape is disclosed. The blood sampling transfer device provides a closed system that reduces the exposure of a blood sample to both skin and environment and provides fast mixing of a blood sample with a sample stabilizer.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID COLLECTION AND TESTING SYSTEM

A blood collection device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the blood collection device separates a plasma portion from a cellular portion. After separation, the blood collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The blood collection device of the present disclosure also provides a closed collection and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The blood collection device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the blood collection device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

BIOLOGICAL FLUID SEPARATION DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid separation device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collection of the blood sample, the plasma portion is separated from the cellular portion. After separation, the biological fluid collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid collection device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid collection device is engageable with a biological fluid testing device for closed transfer of a portion of the plasma portion from the biological fluid collection device to the biological fluid testing device. The biological fluid testing device is adapted to receive the plasma portion to analyze the blood sample.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

In order to facilitate diffusive transfer of an entity such as a solute between immiscible fluids and subsequent separation of the liquids without mixing, method and apparatus are disclosed having first and second flow paths (1, 2) carrying first and second immiscible fluids on opposite sides of a foraminous sheet (8) wherein the height of the apertures (10) therein are not greater than 200 micrometers (measured perpendicular to the width of the sheet and to the direction of fluid flow), and a stable interface is formed between the fluids within each aperture, with a significant amount of fluid flow immediately adjacent the interface. Diffusive transfer takes place across the interface, and subsequently the fluids flow away from the region without mixing. The width of the flow paths measured perpendicular to sheet (8) lies between 10 and 500 micrometers. The walls of each aperture may be parallel or tapered.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

In order to facilitate diffusive transfer of an entity such as a solute between immiscible fluids and subsequent separation of the fluids without mixing, method and apparatus are disclosed having first and second fluid flow paths (11, 12) carrying first and second immiscible fluids, the flow paths communicating with one another in an interface region (16) in which the fluids contact one another and a stable open interface is formed. Diffusive transfer takes place across the interface, and subsequently the fluids flow away from the interface without mixing. The width of the flow paths in the interface region measured normal to the interface lies between 10 and 500 micrometres.

LIQUID ST, COMPRISING POLYMER MARKER, POLYMER APPLICATION MARKER AS TOOL AUTHENTICATION, AS WELL AS METHOD AND DEVICE FOR DETECTING POLYMER MARKER, DISSOLVED IN LIQUID STI

RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

FILTERING DEVICE INTEGRATING THE CONTROL OF VOLUME SAMPLES

AMPLIFYING DEVICE OF CONCENTRATION Of PRESENT ANALYTES IN an ATMOSPHERE AND SYSTEM OF DETECTION ASSOCIATES

METHOD AND APPARATUS FOR AUTOMATIC CELL AND BIOLOGICAL SAMPLE PREPARATION AND DETECTION

A system identifies or screens one or more biological materials. The system includes at least one bioreactor (100) adapted to provide biological samples and an automated sample handler comprising at least one bi-directional pump (220) to transport biological samples, reagents and fluids, at least one reaction chamber (100) with at least two regions separated by a semi-permeable membrane (112) and fitted with a thermoelectric module (130) for cooling and heating the chamber (100). At least one multi-positional valve (222) directs transport of the biological samples, reagents and fluids into and out of the reaction chamber (100) and a micro-processor (240) to control operations of the sample handler. A detector (300) is used to identify the biological material in the samples and a sample repository (514) collects the identified biological samples.

METHOD AND DEVICE FOR DETECTING EXPLOSIVE-SUBSTANCE PARTICLES IN A GAS FLOW

The invention relates to a method and device for detecting explosive-substance particles in a gas flow (46), wherein the gas flow (46) is conducted through an adsorption net (12) for a specified time period, wherein explosive-substance particles (18) are adsorbed onto the adsorption net, the adsorption net (12) is subsequently heated to a heating temperature, at which the explosive-substance particles (18) desorb, and a gas flow containing the desorbed explosive-substance particles is fed to a detector (40) in order to detect the explosive-substance particles, wherein a microfilter (12) having a pore size of less than the particle size of the explosive-substance particles (18) is used as the adsorption net. Thus it is possible to collect practically all explosive-substance particles (18) contained in the gas flow (46) and to feed said explosive-substance particles to a subsequent detection process, whereby the sensitivity of the detection process is increased.

SAMPLE COMPONENT TRAPPING, RELEASE, AND SEPARATION WITH MEMBRANE ASSEMBLIES INTERFACED TO ELECTROSPRAY MASS SPECTROMETRY

The invention provides a method and apparatus for trapping, releasing and/or separating sample components in solution passing through a channel with or without packing material present by passing ion current through the channel driven by an electric field. A portion of the ion current comprises cation and/or anion species generated from second solution flows separated from the sample solution flow path by semipermeable membranes. Cation and/or Anion ion species generated in the second solution flow regions are transferred into the sample solution flow path through ion selective semipermeable membranes. Ion current moving along the sample solution flow path is controlled by varying the composition of the second solutions and/or changing the voltage between membrane sections for a given sample solution composition. The sample composition may also be varied separately or in parallel to enhance trapping, release and/or separation efficiency and range. The invention when interfaced to an Atmospheric ...

METHOD AND APPARATUS FOR THE FILTRATION OF BIOLOGICAL SOLUTIONS

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

Microwave-assisted headspace liquid-phase microextraction of an analyte

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry.

DEVICE FOR DIALYSIS HAVING ACCESS PORT

PROBLEM TO BE SOLVED: To provide a device for dialysis of a small fixed-volume sample. SOLUTION: A device for dialysis of a sample includes a gasket and a sample chamber formed by a gasket and dialysis membranes affixed to each side of the gasket in facing relationship. A housing supports dialysis membrane and gasket. An air chamber causes the device to float in a generally upright position when the device is immersed in a dialysate. An access port is provided through the gasket and between the dialysis membranes. The access port is accessible from outside the housing, and in communication with the sample chamber, so that a dispersing end of a dispensing device can be inserted through the access port dispense sample into the sample chamber. In one embodiment, the gasket is impermeable or essentially impermeable to the sample being dialyzed. COPYRIGHT: (C)2009,JPO&INPIT ...

Ionenmobilitätsspektrometer mit Substanzsammler

Die Erfindung betrifft ein Ionenmobilitätsspektrometer, in dem eine Messröhre, ein Filter und eine Fördereinrichtung zu einem geschlossenen Gaskreislauf verbunden sind, wobei Substanzen aus einem Probgas über einen Membraneinlass in den Gaskreislauf eingeführt werden. Die Erfindung besteht darin, dass ein Substanzsammler nicht außerhalb, sondern innerhalb des Gaskreislaufs angeordnet ist.

Verfahren und Vorrichtung zur Abreicherung von gelösten, flüchtigen Komponenten in Getränken

Vorrichtung für die Aufbereitung eines Gasstromes vor der Zufuhr desselben zu einem Massenspektrometer

Die Erfindung betrifft eine Vorrichtung für die Aufbereitung eines Gasstromes vor der Zufuhr desselben zu einem Massenspektrometer, wobei der Gasstrom einen oder mehrere Analyten und als Trägergas Helium enthält. Erfindungsgemäß ist eine selektiv wirkende Trenneinrichtung zum Abtrennen eines Teils des Trägergases aus dem Gasstrom (10) und zur Bildung eines Restgasstromes (11) und eines hiervon abgetrennten Trägergasstromes (12) vorgesehen, wobei im Restgasstrom ein höherer Anteil des Analyten vorliegt als im Gasstrom und wobei im abgetrennten Trägergasstrom ein niedrigerer Anteil des Analyten vorliegt als im Gasstrom.

A device for removing dissolved gas or volatile component from a liquid sample

Improvements in and relating to sample collection

PROCEDURE FOR THE PRODUCTION OF A PROTEOMANALYSELÖSUNG

ULTRAFILTRATION DEVICE FOR DRUG BINDING STUDIES

Methods for the detection of nitric oxide in fluid media

Methods for the detection of nitric oxide in fluid media

Method and apparatus for diffusive transfer between immiscible fluids

Methods and apparatus to selectively extract constituents from biological samples

Methods and apparatus provide filtration for concentrating analytes, such as bacteria or exosomes, of a biological sample, such as blood or urine. The technology may employ membrane devices that implement one or more tangential flow filtration processes such as in stages. An example membrane device may typically include a membrane having sides and ends. The membrane may selectively permit constituent(s) of the sample to pass through while retaining other constituents at one side. An input chamber of the device may include an inlet near one end and an outlet near the other end, and that may permit a tangential flow of the sample along the first side surface, and a trans-membrane passing of constituent(s). An output chamber of the device may be configured at the second side surface to receive the passing constituents. Such devices may be provided in a kit to facilitate targeting of a desired biological analyte concentration.

MICROFLUIDIC SYSTEMS FOR SIZE BASED REMOVAL OF RED BLOOD CELLS AND PLATELETS FROM BLOOD

The invention features devices and methods for enriching a sample in one or more desired particles. An exemplary use of these devices and methods is for the enrichment of cells, e.g., white blood cells in a blood sample. In general, the methods of the invention employ a device that contains at least one sieve through which particles of a given size, shape, or deformability can pass. Devices of the invention have at least two outlets, and the sieve is placed such that a continuous flow of fluid can pass through the device without passing through the sieve. The devices also include a force generator for directing selected particles through the sieve. Such force generators employ, for example, diffusion, electrophoresis, dielectrophoresis, centrifugal force, or pressure-driven flow.

HOLLOW FIBER MEMBRANE SAMPLE PREPARATION DEVICES

Simultaneous sample purification, enrichment and analysis of pharmaceuticals, illicit drugs, pollutants, biotechnological products, synthetic organic reaction products and food/flavor ingredients from complex matrices can be performed using porous hollow fiber or porous-disk liquid-membrane devices. The devices are part of a multi-well (e.g. 96-well) plate. The devices can be used for selective separation and enrichment of complex mixtures containing trace levels of analytes, and can be used in tandem with analytical instruments which routinely handle multiple samples under high throughput screening conditions. A multi-well/multi-vial plate can into state-of-the-art HPLC or GC sampling systems or LC/MS or GC/MS instruments. Samples can be enriched several orders of magnitude and can directly be withdrawn from the fiber and injected into the chromatographic instruments. Alternatively, these enriched samples can be introduced directly into MS, CE or other detection devices. Selective extraction ...

MOLECULAR WEIGHT FILTRATION SYSTEM AND APPARATUS

A molecular filtration device and method of use capable of filtering and purifying molecules of a particular characteristic, wherein the amount of molecule to be filtered may be in the nanogram range and may be dispersed in a relatively large volume of solution. The resultant elution may include a substantially pure solution comprising the desired molecule.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid collection device adapted to receive a multi-component blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to separate the plasma portion from the cellular portion. After separation, the biological fluid collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid collection device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid collection device is engageable with a testing device for closed transfer of a portion of the plasma portion from the biological fluid collection device to the testing device. The testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collection of the blood sample, the plasma portion is separated from the cellular portion. After separation, the biological fluid collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid collection device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid collection device is engageable with a biological fluid testing device for closed transfer of a portion of the plasma portion from the biological fluid collection device to the biological fluid testing device. The biological fluid testing device is adapted to receive the plasma portion to analyze the blood sample.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID SEPARATION SYSTEM

A biological fluid separation system for a blood sample is disclosed. The biological fluid separation system includes a biological fluid collection device adapted to receive a blood sample and a centrifuge. The centrifuge is adapted to receive the biological fluid collection device such that with the biological fluid collection device received within the centrifuge and a rotational force applied to the biological fluid collection device, a plasma portion of the blood sample is separated from a cellular portion of the blood sample. The biological fluid collection device is only receivable within the centrifuge in one orientation.

METHODS FOR THE DETECTION OF NITRIC OXIDE IN FLUID MEDIA

In accordance with the present invention, non-invasive methods have been developed for the measurement of NO levels in a variety of fluid media, e.g., in mammalian fluids. The present invention embraces the use of a semipermeable membrane bag containing a nitric oxide reacting substance to trap NO diffusing in to the bag. The permeability of selected semi-permeable membranes to nitric oxide, but not to nitrate/nitrite, makes is possible for the semipermeable membranes bags employed in the practice of the present invention to selectively collect NO, even in the presence of potentially competing species such as nitrate and nitrite. The simple, easy and non-invasive methods of the invention for the measurement of NO levels in fluid media will find a variety of uses, e.g., for diagnosis and monitoring of NO overproduction or underproduction that has been associated with many inflammatory and infectious diseases.

Biological fluid sampling transmission device and biological fluid separation with the inspection system

DEVICE AND METHOD FOR EXTRACTING AT LEAST ONE GAS DISSOLVED IN A LIQUID

L'invention concerne un dispositif d'extraction (1, 101) d'au moins un gaz dissout dans un liquide, ledit dispositif comprenant (i) au moins une membrane (3, 103) séparatrice gaz-liquide ; et une méthode de mesure continue de la concentration ou la pression partielle d'au moins un gaz dissout dans un liquide, ladite méthode comprenant la mise en contact d'un dispositif de séparation gaz/liquide comprenant au moins une membrane avec un liquide dont la concentration d'au moins un gaz dissout est à mesurer, la séparation d'au moins un gaz dissout dans le liquide au travers la ou les membranes du dispositif de séparation gaz/liquide, la mesure du flux de diffusion et/ou de perméation au travers la ou les membranes, et le calcul de la concentration ou de la pression partielle de gaz préalablement dissout dans le liquide à partir du flux de diffusion et/ou de perméation.

폐암 환자의 혈중 순환 종양세포를 활용한 EGFR-TKI 내성 환자의 맞춤형 항암제 선별시스템 및 방법

... 본 발명은 폐암 환자의 혈중 순환 종양세포(CTCs; circulating tumor cells)를 활용한 상피세포 성장인자 수용체 티로신 키나아제 저해제(EGFR-TKI; epidermal growth factor receptor-tyrosin kinase inhibitor) 내성 환자의 맞춤형 항암제 선별시스템 및 선별방법에 관한 기술로서, 더욱 상세하게는 희소 세포 분리장치를 활용하여 상피세포 성장인자 수용체 티로신 키나아제 저해제 내성 환자의 혈액으로부터 혈중 순환 종양세포를 분리한 후, 항암제 반응 검사를 통하여 다양한 후보 항암제 중에서 최적의 개인 맞춤형 항암제를 선별하는 시스템 및 방법에 관한 것이다.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

BIOLOGICAL FLUID TRANSFER DEVICE AND BIOLOGICAL FLUID SAMPLING SYSTEM

A biological fluid sampling transfer device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid sampling transfer device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid sampling transfer device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

DEVICES AND METHODS FOR CONCENTRATION AND ANALYSIS OF FLUIDS

Disclosed are articles, compositions and methods for detecting analytes. The disclosed articled, compositions and methods increase the ease of detection and quantitation of the target analyte.

Dialysis cell and tray for dialysis cells

A dialysis cell is provided for the measurement of free thyroxine. The dialysis cell preferably includes a polyhedral housing including a top, a bottom and four sides. The dialysis cell is made up of a buffer portion and serum portion. The buffer portion includes a cavity and the serum portion includes a cavity which, when the buffer portion and serum portion are assembled together, form a central chamber. The central chamber is divided by a vertically aligned dialysis membrane held in place by two O-rings. Buffer is introduced into the dialysis cell's buffer portion through a buffer inlet extending from the dialysis cell's top side to the buffer portion's cavity. Similarly, serum is introduced into the serum portion through an inlet which extends from an opening formed on the dialysis cell's top to the serum portion's cavity.

METHOD AND APPARATUS FOR THE FILTRATION OF BIOLOGICAL SOLUTIONS

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.

УСТРОЙСТВО МОЛЕКУЛЯРНОГО ОБМЕНА

... 1. Устройство молекулярного обмена, включающее в себя корпус, который располагается от проксимального конца до дистального конца, обеспечивая поддержку по меньшей мере двум проводящим каналам для жидкостей, которые располагаются от проксимального конца к дистальному концу; корпус оснащен по меньшей мере одной обменной апертурой между дистальным концом и проксимальным концом, где участок проводящего канала для жидкостей, выходящий из обменной апертуры, является пористым. ! 2. Устройство молекулярного обмена по п.1, где разделитель располагается вдоль корпуса по меньшей мере на длину обменной апертуры, разделяя по меньшей мере два проводящих канала для жидкостей. ! 3. Устройство молекулярного обмена по п.2, где разделитель располагается главным образом вдоль всей длины корпуса от дистального конца к проксимальному концу, разделяя по меньшей мере два проводящих канала для жидкостей. ! 4. Устройство молекулярного обмена по п.2, где разделитель располагается вдоль центральной оси корпуса, и ...

DIALYSEVORRICHTUNG MIT LUFTKAMMER

Ion mobility spectrometer with substance collector

Device for preparing a gas flow for introduction thereof into a mass spectrometer

The invention relates to a device for preparing a gas flow for introduction thereof into a mass spectrometer, wherein the gas flow contains one or more analytes and has helium as carrier gas. According to the invention, a selective separating device is provided for separating off a part of the carrier gas from the gas flow (10), to form a residual gas flow (11) and a separated carrier gas flow (12). A higher fraction of the analyte is present therm than in the gas flow a nd in the separated carrier gas flow there is a lower fraction of the analyte.

A monitoring system

A delivery system 1 and method comprises: a receptacle 2 for containing a fluid, adapted to allow the fluid to flow from the receptacle; a molecular exchange area 4; and a supply conduit 3 defining a fluid path between from the receptacle to the molecular exchange area, wherein the receptacle is positioned above the other components such that the fluid is transported by the action of gravity. In a further embodiment the system and method is a monitoring system and method, further comprising an outlet conduit 5 defining a fluid path from the molecular exchange area to a sensor unit 10. The systems may comprise one or more attachments to hold one or more of the components of the system in position (e.g. a stand, pole, and/or wall mount), and may also comprise one or more means to control the flow rate of the fluid (e.g. a constriction element, moving the height of the receptacle with respect to the molecular exchange area, altering the size or internal cross sectional area of the supply conduit ...

Molecular exchange device

Method of separating a multiphase mixture

A fluid separation method comprises separating a multiphase mixture 106 with a porous membrane 108 in a micro-fluidic device wherein a pressure difference is maintained across the membrane below a capillary breakthrough pressure of a non-wetting component of the multiphase mixture; wherein the separating includes flowing the multiphase mixture across the membrane in a direction parallel to the membrane such that the flowing of a liquid of interest is from the multiphase mixture through the membrane at a flow rate at least one or two orders of magnitude lower than a flow rate of the multiphase mixture passing the membrane. The passing of the liquid through the membrane prevents fouling without backflushing. Fluid analysis is accomplished with microfluidic devices and may be reported in real-time or near real-time in a subterranean environment. In addition or alternative to oilfield applications, the principles of the present invention contemplate separation in a laboratory or other environment ...

Automated crossflow filtration method and system

Device for preparing a gas flow for introduction thereof into a mass spectrometer

PROCEDURE AND VORICHTUNG FUR DIFFUSION EXCHANGE BETWEEN NOT MIXABLE LIQUIDS

Molecular exchange device

The present application relates to a molecular exchange device (1) for use with an analysis and control apparatus and a method of manufacturing a molecular exchange device. The molecular exchange device comprises a casing (2), extending from a proximal end (3) to a distal end (4), supporting at least two fluid passageways (7a, 7b) extending from the proximal end to the distal end; the casing comprising at least one exchange aperture (9a, 9b) between the distal end and the proximal end, wherein a portion of the fluid passageway exposed by the exchange aperture is porous.

Molecular exchange device

Capillary pressure re-set mechanism and applications

Many hand-held diagnostics are limited in their functionality due to the challenging physics associated with small dimensional systems. An example of this is capillary forces in hydrophilic systems, such as the tight retention of liquid passing through a small pore filtration membrane, or capillary force driven micro fluidics where, to keep liquid flowing the dimensions of the system become so small that the flow rates are too low to be useful, or the manufacturing of such devices becomes uneconomical. This disclosure details methods to 'reset' the capillary force condition to avoid the requirement of transient pressure spikes associated with the breakthrough pressure of small pore membranes, and avoid the necessity of extremely small microfluidic channels, which can be useful in applications such as filtration of whole blood to plasma using only suction pressure or passive capillary pressure.

CELL CONCENTRATION AND PATHOGEN RECOVERY

Methods and kits for the isolation of organisms. Such methods and kits are particularly useful for concentrating and recovering viable organisms from food material. The recovered organisms are of sufficient number and purity to allow detection using a biochip device.

RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability.

MEDICAL DEVICE FOR COLLECTION OF A BIOLOGICAL SAMPLE

A biological fluid sampling device for collecting a blood sample from a separate vascular access device and for ejecting a portion of the collected sample to a point-of-care testing device for analysis is provided. The biological fluid sampling device includes a body enclosing a reservoir. The reservoir has an internal volume sufficient to contain enough blood for use in a diagnostic test. The sampling device further includes: an access lumen extending from a distal end of the body for establishing fluid communication between a separate vascular access device and the reservoir; an outflow lumen also in fluid communication with the reservoir; and a removable vented cap attached to the outflow lumen including a gas permeable vent in gaseous communication between the reservoir and ambient air. In addition, several sample and transfer devices are provided for obtaining a sample from a subject and transferring the sample to a point-of-care testing device.

DIALYSIS DEVICE WITH AIR CHAMBER

A device (10) for the dialysis of a sample includes a hermetically sealed sample chamber (18) formed by a gasket (12) with dialysis membranes (14, 16) affixed to each side in facing relationship. The gasket (12) is impermeable to the sample being dialyzed, but is penetrable and reusable such that a sample introduction mechanism can be inserted through the gasket (12) into the chamber, and then withdrawn without sample being permitted to leak. The device (10) is fitted into a rigid housing containing windows (24, 26) and at least one port (50) parallel to the dialysis membranes for directing the sample introduction mechanism into the gasket. The housing includes at least one pressure ridge (40) for assisting in hermetically sealing the sample chamber and air chamber (46) at one end for causing the device to float in an upright position when immersed in a dialysate.

BIOLOGICAL FLUID TRANSFER DEVICE AND BIOLOGICAL FLUID SAMPLING SYSTEM

A biological fluid sampling transfer device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid sampling transfer device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid sampling transfer device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

BIOLOGICAL FLUID COLLECTION DEVICE AND BIOLOGICAL FLUID SEPARATION AND TESTING SYSTEM

A biological fluid collection device adapted to receive a multi-component blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to separate the plasma portion from the cellular portion. After separation, the biological fluid collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid collection device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid collection device is engageable with a testing device for closed transfer of a portion of the plasma portion from the biological fluid collection device to the testing device. The testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

METHOD AND DEVICE FOR DETECTING EXPLOSIVE-SUBSTANCE PARTICLES IN A GAS FLOW

The invention relates to a method and device for detecting explosive-substance particles in a gas flow (46), wherein the gas flow (46) is conducted through an adsorption net (12) for a specified time period, wherein explosive-substance particles (18) are adsorbed onto the adsorption net, the adsorption net (12) is subsequently heated to a heating temperature, at which the explosive-substance particles (18) desorb, and a gas flow containing the desorbed explosive-substance particles is fed to a detector (40) in order to detect the explosive-substance particles, wherein a microfilter (12) having a pore size of less than the particle size of the explosive-substance particles (18) is used as the adsorption net. Thus it is possible to collect practically all explosive-substance particles (18) contained in the gas flow (46) and to feed said explosive-substance particles to a subsequent detection process, whereby the sensitivity of the detection process is increased.

Biological fluid collection device and biological fluid collection and test system

The pre-processing device

Device and process for passive sampling

The present invention generally relates to the field of monitoring or analysing an aqueous system (both water or sediment) for analytes and in particular to processes and devices for sampling and analysing the concentration or levels of specific analytes or solutes in an aqueous system such as a natural water system (e.g., rivers, ponds, etc.) or industrial effluent streams. The analytes or solutes may or may not be pollutants of said aqueous systems. The sampling device comprises a polymer inclusion membrane or a composite polymer inclusion membrane.

PROCESS AND DEVICE OF DETECTION AND QUANTIFICATION Of ONE COMPOSE CHEMICAL IN a FLUID CURRENT

L'invention concerne un procédé permettant de capter un composé chimique, typiquement une impureté, et d'accumuler ce composé chimique sur une masse de captation CAPT comprise dans au moins une cartouche adsorbante démontable MC pendant une longue durée excédant 2 jours, en vue de son analyse chimique externe. L'invention concerne aussi un dispositif ST adapté de captation d'au moins un composé chimique dans un courant fluide, pour sa détection et/ou sa quantification, comprenant en aval de cette ou de ces cartouches, au moins un moyen de mesure du débit cumulé ayant traversé cette ou ces cartouches pendant un intervalle de temps déterminé. Selon une variante préférée de l'invention, la masse de captation CAPT est fragmentée en une pluralité de masses élémentaires CAPTi, permettant d'évaluer l'étalement d'un front d'adsorption irréversible ou le déplacement d'une zone chromatographique.

BIOMOLECULE CONCENTRATING DEVICE

The present invention relates to a biomolecule concentrating device. According to one embodiment of the present invention, the concentrating device comprises: a first layer comprising a first buffer reservoir, a second buffer reservoir, and a sample reservoir formed between the first buffer reservoir and the second buffer reservoir; ad a microchannel attached to a lower surface of the first layer and formed between the first buffer reservoir and the second buffer reservoir with the sample reservoir interposed therebetween. When a potential difference is generated across the microchannel, a target reservoir can be concentrated in the sample reservoir. COPYRIGHT KIPO 2017 ...

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

CELL SEPARATION CHIP AND CELL SEPARATION METHOD USING SAME

Disclosed are a cell separation chip and a cell separation method using the same. The cell separation chip according to the present invention for separating a cell from a solution including cells is characterized by comprising an upper plate, a membrane located on a certain area of the upper plate, and an absorption means located on the lower end of the upper plate, and absorbing a solution which passed through the membrane of the solution including cells. According to the present invention, the solution including cells is absorbed without applying and maintaining an additional external voltage, thereby having an effect of efficiently collecting and separating only a cell in the solution. COPYRIGHT KIPO 2016 ...

Method for detecting albumin based on colorimetric and system thereof

ON-SITE KIT FOR ANALYSIS OF DISINFECTANT BYPRODUCTS SPECIES AND AMOUNTS THEREOF IN DRINKING WATER SUPPLIES

A portable kit that permits reliable and quantifiable analysis of trihaloamethanes and haloacetic acids within drinking water samples utilizing a handheld (portable) fluorescing detection instrument for simultaneous measurements of such species is provided. With the necessity to chlorinate drinking water to remove harmful bacteria and other potential toxins, trihalomethane and haloacetic acid bypro ducts are generated during such a disinfecting procedure that may harm humans after consumption as well due to highly suspect carcinogenicity. The inventive kit-based analytical method of the invention has been found to be nearly as reliable as source measuring methods for the same purpose, providing a relatively quick measuring method that may be undertaken at any drinking water source location.

CELL CONCENTRATION AND PATHOGEN RECOVERY

Methods and kits for the isolation of organisms. Such methods and kits are particularly useful for concentrating and recovering viable organisms from food material. The recovered organisms are of sufficient number and purity to allow detection using a biochip device.

METHODS FOR FABRICATING ELECTROKINETIC CONCENTRATION DEVICES

The present invention provides a device and methods of use thereof in concentrating a species of interest and/or controlling liquid flow in a device. The methods make use of a device comprising a fluidic chip comprising a planar array of channels through which a liquid comprising a species of interest can be made to pass with at least one rigid substrate connected thereto such that at least a portion of a surface of the substrate bounds the channels, and a high aspect ratio ion-selective membrane is embedded within the chip, attached to at least a portion of the channels. The device comprises a unit to induce an electric field in the channel and a unit to induce an electrokinetic or pressure driven flow in the channel.

Method for determining pectin content in plant sample

A method for determining pectin content in a plant sample includes: 1) adding an acidic alcohol solution to the plant sample, heating in a water bath and filtration; 2) soaking residue from step 1 with an acidic solution, heating in a water bath, a second filtration, bringing to volume after cooling and obtaining filtrate; 3) adding an acetic acid/sodium acetate buffer solution to residue of step 2, adding a pectinase solution, heating under vibration in a water bath, and a third filtration to obtain a filtrate; 4) adding an acetic acid/sodium acetate buffer solution and a pectinase solution to filtrate from step 2, heating the mixture under vibration in a water bath to obtain an enzymatic hydrolysate, adding filtrate obtained in step 3 to the enzymatic hydrolysate, bringing to volume, and obtaining a test solution; 5) drawing the test solution into a continuous flow analyzer to perform analysis.

Biological Fluid Transfer Device and Biological Fluid Sampling System

A biological fluid sampling transfer device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid sampling transfer device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid sampling transfer device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid transfer device adapted to receive a multi-component blood sample , the biological fluid transfer device comprising:a housing having an inlet port and a transfer port, the inlet port and the transfer port in fluid communication;a mixing channel in fluid communication with the inlet port and the transfer port and shaped to promote mixing of the multi-component blood sample; anda blood separation element disposed between the inlet port and the transfer port, the blood separation element adapted to restrain a first component of the multi-component blood sample and allow a second component of the multi-component blood sample to pass therethrough.2. The biological fluid transfer device of claim 1 , wherein the first component is a cellular portion of the multi-component blood sample and the second component is a plasma portion of the multi-component blood sample.3. The biological fluid transfer device of claim 1 , wherein the mixing channel comprises a sample stabilizer.4. The biological fluid transfer ...

Blood Sampling Transfer Device

A blood sampling transfer device that includes a lancing tape having a flow channel and a transfer cartridge removably connected to the lancing tape is disclosed. The blood sampling transfer device provides a closed system that reduces the exposure of a blood sample to both skin and environment and provides fast mixing of a blood sample with a sample stabilizer.

SMART DATA SUBSURFACE DATA REPOSITORY SYSTEM, METHOD AND COMPUTER PROGRAM PRODUCT

A system, method and computer program product for source area contamination data acquisition, analysis and processing. The present invention leverages and expands direct sensing technology, knowledge and experience to provide detailed, real-time images of subsurface conditions. The latest technologies in sensors, digital processing, computation and 3D visualization are used to enable clients to work with a single contractor who can perform data acquisition, processing and analysis necessary to produce quantifiable, user-friendly 3D maps on a daily basis which can be delivered via the Internet and/or to mobile devices. This allows the owner and site project manager to make timely decisions as they guide investigation, remediation and monitoring efforts.

Method for detecting albumin based on colorimetric assay and system thereof

A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

METHOD FOR RECOVERING MICROBIAL CELLS

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.

METHOD AND APPARATUS FOR DIFFUSIVE TRANSFER BETWEEN IMMISCIBLE FLUIDS

VERFAHREN UND VORICHTUNG FUR DIFFUSIONSAUSTAUSCH ZWISCHEN NICHT MISCHBARE FLÜSSIGKEITEN

VERFAHREN UND VORICHTUNG FUR DIFFUSIONSAUSTAUSCH ZWISCHEN NICHT MISCHBARE FLÜSSIGKEITEN

Hand-held trace vapor/particle sampling system

A sampling system that contains filter components for collecting and concentrating vapor and particles in high-volume flows. The sample is then vaporized and delivered to a detector at a low-volume flow. The invention also has a sampling probe that contains an air-jet to help dislodge particles from surfaces and a heating lamp to help vaporize compounds on surfaces or objects. The sampling system is especially useful for screening for explosives and other illicit chemicals and toxins on people, baggage, cargo, and other objects.

Box producing apparatus, inspection unit, and print register control method for a box producing apparatus

An box producing apparatus is disclosed, including a camera that obtains an image of a printed face which is printed on a box material sheet by the printing section; and a register correction control unit that compares a specific picture in a master image of a sample for the print, and a picture in a region specified according to the specific picture in the image of the printed face obtained by the camera to determine an amount of register displacement in each of the print units, and controls a register adjustment mechanism provided in each of the print units based on the amount of register displacement to correct a register of the print.

COLUMNS FOR INCUBATION AND ISOLATION OF CHEMICAL AND/OR BIOLOGICAL SAMPLES

The present invention discloses a column for incubating and/or isolating chemical and/or biological samples. Furthermore, a method for incubating and/or isolating chemical and/or biological samples as well as a method for separating liquids and solids from a disperse mixture is subject-matter of the present invention. 1. A method for releasing a chemical and/or biological sample from a solid support comprising the steps: [{'b': '1', '(i) a feed opening ();'}, {'b': 2', '12, '(ii) an outlet opening (,) with closing means;'}, 'wherein the closing means is permeable for liquids only when an external pressure is applied;', '(b) incubating the sample with butlers and/or reagents for releasing the biological sample from the solid support for appropriate time and under appropriate conditions;', '(c) applying an external pressure to the closing means of the column; wherein the pressure is applied through centrifugation;', '(d) collecting the buffer and/or reagents containing the biological sample; and', '(e) optionally collecting the solids and repeating steps (a) to (d) as desired., '(a) applying a solid support comprising said biological sample in a column, wherein the column comprises2. The method according to claim 1 , wherein pressure of at least 250 kPa is applied to at least 400 kPa.3. The method according to claim 1 , wherein the centrifugation is performed with a fixed angle rotor.4. The method according to claim 3 , wherein column has an angle in the fixed angle rotor relative to the rotational axis between 20° and 70°.51. The method according to wherein the outlet opening () of the column is smaller than the feed opening.641424344454. The method according to wherein the closing means comprises at least one predetermined breaking point ( claim 1 , claim 1 , claim 1 , claim 1 , claim 1 , ) claim 1 , wherein the at least one breaking point opens when the external pressure is applied.743. The method according to claim 6 , wherein the closing means is a flexible seal ...

METHOD AND DEVICE FOR DETECTING EXPLOSIVE-SUBSTANCE PARTICLES IN A GAS FLOW

A method for detecting explosive substance particles in a gas flow includes passing the gas flow through an adsorption net for a specified time period so as to adsorb explosive-substance particles in the gas flow on the adsorption net. The adsorption not includes a microfilter having a pore size that is smaller than the particle size of the explosive-substance particles. The adsorption net is heated to a heating temperature so as to desorb the explosive-substance particles from the adsorption net. A gas flow comprising the desorbed explosive-substance particles is supplied to a detector so as to detect the explosive-substance particles. 110-. (canceled)11. A method for detecting explosive substance particles in a gas flow , the method comprising:passing the gas flow through an adsorption net for a specified time period so as to adsorb explosive-substance particles in the gas flow on the adsorption net, the adsorption net including a microfilter having a pore size that is smaller than a particle size of the explosive-substance particles;heating the adsorption net to a heating temperature so as to desorb the explosive-substance particles from the adsorption net;supplying a gas flow comprising the desorbed explosive-substance particles to a detector so as to detect the explosive-substance particles.12. The method according to claim 11 , wherein the microfilter has a pore size of less than 1 μm.13. The method according to claim 12 , wherein the microfilter has a pore size of less than 400 nm.14. The method according to claim 11 , wherein the heating temperature is set and the microfilter has a pore size configured such that the explosive-substance particles pass through the microfilter in a gaseous phase after the heating and desorption.15. The method according to claim 11 , wherein the microfilter is heated to a particular temperature so as to detect particular explosive substances.16. The method according to claim 11 , wherein the passing the gas flow through the ...

Method for collecting droplet attached on external surface of needle into capillary tube

In order to provide a method for collecting a droplet attached on an external surface of a needle into a capillary tube, the present invention is a method for collecting a droplet attached on an external surface of a needle into a capillary tube, the method comprising steps of (a) preparing a substrate comprising a capillary tube; a flexible thin film; a liquid-repellent film; and a hole; (b) moving the needle in the Z-direction to move the droplet from the external surface of the needle to the surface of the liquid-repellent film; (c) allowing the droplet to arrive at an inlet of the capillary tube by moving the needle more in the Z-direction, so as to suck the droplet into the capillary tube by a capillary phenomenon.

METHODS FOR FABRICATING ELECTROKINETIC CONCENTRATION DEVICES

The present invention provides a device and methods of use thereof in concentrating a species of interest and/or controlling liquid flow in a device. The methods make use of a device comprising a fluidic chip comprising a planar array of channels through which a liquid comprising a species of interest can be made to pass with at least one rigid substrate connected thereto such that at least a portion of a surface of the substrate bounds the channels, and a high aspect ratio ion-selective membrane is embedded within the chip, attached to at least a portion of the channels. The device comprises a unit to induce an electric field in the channel and a unit to induce an electrokinetic or pressure driven flow in the channel. 123-. (canceled)24. A method of concentrating a species of interest in a liquid , the method comprising applying said liquid comprising said species of interest to a concentrating device comprising:a fluidic chip comprising a planar array of channels through which a liquid comprising a species of interest can be made to pass;at least one rigid substrate connected thereto such that at least a portion of a surface of said substrate bounds said channels; anda high aspect ratio ion-selective membrane embedded within said chip, attached to at least a portion of said channels.25. The method of claim 24 , further comprising the steps of:inducing an electric field in said channel whereby ion depletion occurs in a region in said channel proximal to said high aspect ratio ion-selective membrane, and a space charge layer is formed within said channel, which provides an energy barrier to said species of interest; andinducing liquid flow in said channel.26. The method of claim 25 , wherein said flow is electroosmotic.27. The method of claim 25 , wherein said flow is pressure driven.28. The method of claim 25 , wherein steps are carried out cyclically.29. The method of claim 25 , wherein inducing an electric field in said channel is by applying voltage to said ...

POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES

A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications. 126-. (canceled)27. A filtration device comprising:an inlet layer including a first volume and a first inlet layer opening to said first volume;a filter structure including a polymer filter layer having a first filter surface and a second filter surface diametrically opposite to said first filter surface; andan outlet layer including a second volume and a first outlet layer opening to said second volume,wherein said first filter surface is exposed to said first volume,said second filter surface is exposed to said second filter volume, andat least a filtered portion of a sample entering said first inlet layer opening, passes from said first volume to said second volume via said filter layer and exits said second volume via said first outlet layer opening.28. The filtration device of claim 27 , further comprising:a top layer covering at least a portion of said first volume opposite said first surface of said filter layer; anda base layer covering at least a portion of said second volume opposite said second surface of said filter ...

Devices and methods for concentration and analysis of fluids

Disclosed are articles, compositions and methods for detecting analytes. The disclosed articled, compositions and methods increase the ease of detection and quantitation of the target analyte.

Microfluidic extraction device having a stabilized liquid/liquid interface

A microfluidic device for extraction of analytes of interest from a carrier liquid in a liquid solvent, the two liquids forming an interface between micro-pillars in an extraction chamber. The carrier liquid forms a wetting angle θ1 on the micro-pillars and a bottom wall of the extraction chamber, and a wetting angle θ2 on a top wall, the wetting angles satisfying equation 45°≦(θ1+θ2)/2≦135°.

CELL TRAPPING DEVICE

A cell trapping device includes a housing that includes an inlet opening connected to an inlet line through which a cell dispersion liquid is introduced and an outlet opening connected to an outlet line through which the cell dispersion liquid is discharged; and a filter which is positioned within the housing and includes a trapping region for trapping cancer cells contained in the cell dispersion liquid. The filter is bonded to the housing, at least a part of the trapping region is formed of an observation region for observing the trapping region from the outside, the inlet line and the inlet opening are arranged at outer positions than the observation region when viewed from a normal line direction of the filter, and the inlet line is extended along an in-plane direction of the filter. 1. A cell trapping device comprising:a housing that includes an upper member and an lower member, wherein the upper member includes an inlet opening connected to an inlet line through which a cell dispersion liquid is introduced, and the lower member which includes an outlet opening connected to an outlet line through which the cell dispersion liquid is discharged; anda filter which is positioned within the housing and includes a trapping region for trapping cancer cells contained in the cell dispersion liquid,wherein the filter is bonded to the housing,at least a part of the trapping region is formed of an observation region for observing the trapping region from the outside,the inlet line and the inlet opening are arranged at outer positions than the observation region when viewed from a normal line direction of the filter, andthe inlet line is extended along an in-plane direction of the filter.2. The cell trapping device according to claim 1 ,wherein the inlet line and the inlet opening are arranged at outer positions than the trapping region when viewed from the normal line direction of the filter.3. The cell trapping device according to claim 1 ,wherein the filter is ...

LIQUID TESTING SYSTEM, DEVICES, AND METHODS

A testing system and test cartridge for analyzing a sample of water from a water source for specific analyte levels. The test cartridge including a membrane filter that captures a target analyte while allowing a labelled conjugate to permeate through the membrane. The conjugate includes an analyte-specific labelled binding reagent to bind with the target analyte for optical detection. The direct membrane interrogation (i.e., on-filter detection), determines analyte levels without elution of the analyte from a filter thereby improving analyte recovering and assay sensitivity. 1. A method for testing a fluid source for a target analyte using a fluid analyte level assay device , the method comprising:passing a sample of fluid from the fluid source through a filter membrane for the testing;passing a conjugate of labels and analyte-specific binding reagents through the sample-passed filter membrane to bind with a target analyte captured on the filter membrane; andinterrogating the sample-passed filter membrane for the labels bound to the target analyte to determine a level of the target analyte in the sample.2. The method of claim 1 , further comprising:loading a test cartridge including the filter membrane.3. The method of claim 1 , further comprising:preparing the sample-passed filter membrane by washing the sample-passed filter membrane with solution.4. The method of claim 3 , wherein preparing the sample-passed filter membrane further comprising:drying of the washed sample-passed filter membrane prior to interrogation.5. The method of claim 1 , wherein the sample is collected from a cooling tower.6. The method of claim 1 , wherein the labels are up-converting nanoparticles.7. The method of claim 1 , wherein determining the level of the target analyte includes exciting the labels and optically detecting the excited labels to determine the level of the target analyte.8. The method of claim 1 , wherein the target analyte is a bacteria or virus.9. The method of claim 1 , ...

SALIVA COLLECTION, PROCESSING, STABILIZATION, AND STORAGE METHOD

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and/or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness. 1. A method for stabilizing nucleic acid and protein samples isolated from a saliva sample , the method comprising:a) collecting a saliva sample from a subject;b) filtering the saliva sample to produce a filtered sample that is free of cells;c) collecting the filtered sample in at least a first and a second receiving device;d) adding an alcohol solution to the first receiving device to produce an alcohol-containing filtered sample comprising a protein sample, with the proviso that alcohol is not added to the second receiving device to produce an alcohol-free filtered sample comprising a nucleic acid sample; wherein the protein sample and the nucleic acid sample are stabilized for at least 3 days when stored at 25 degrees Celsius; ande) performing an analysis on the filtered sample collected in the first and second receiving devices comprising one or more of: a protein analysis on the alcohol-containing filtered sample or a nucleic acid analysis on the alcohol-free filtered sample.2. The method of claim 1 , wherein the nucleic acid is DNA.3. The method of claim 1 , wherein the nucleic acid analysis is polymerase chain reaction (PCR).4. The method of claim 1 , wherein the nucleic acid is RNA.5. The method of claim 4 , wherein the nucleic acid analysis is RT-PCR.6. The ...

A METHOD OF DETECTING AND DIAGNOSING THE PROGRESSION OF DIABETES

The subject of the invention is the method of detecting and diagnosing the progression of diabetes using Raman spectroscopy which involves the examination of the changes in the composition of urinary extracellular vesicles which confirm the existence of the condition and its progression. The invention can be applied in clinical practice, in particular in the early clinical diagnostics of diabetes and in the monitoring of its progression, in particular diabetic nephropathy and advanced renal impairment caused by diabetes. 1. The method of detecting and diagnosing diabetes wherein the change in the composition of microvesicles in a tissue or liquid sample collected from a patient is examined , and this change confirms the presence of diabetes and its progression.2. The method according to wherein the change in the composition of urinary extracellular vesicles (UEV) is examined.3. The method according to wherein there are the following stages:a) Urinary extracellular vesicles (UEV) are isolated from the urine sample,b) Raman spectra are registered and the analysis of the distribution of the intensity of characteristic bands is performed,c) If it is confirmed that the value of RI for the intensity of the Raman spectrum is lower than the value of RI for the Raman spectrum obtained in an identical way for the sample collected from a healthy individual, the patient is diagnosed with diabetes.4. The method according to wherein the urine sample is subject to centrifugation at 2000×g for about 30 minutes in stage a).5. The method according to wherein claim 3 , in stage a) claim 3 , the urine sample is concentrated using a dialysis membrane with large-diameter pores permeable for the molecules of the average molecular weight below 1000 Da (MWCO) claim 3 , which is followed by washing.6. The method according to wherein the washing solution contains silver chloride and sodium dichloroisocyanurate in the amount of 1 mg of silver chloride and 4.5 mg of sodium dichloroisocyanurate ...

METHODS FOR ANALYZING RESPIRABLE PARTICLES IN BULK MATERIALS

Provided is a method for detecting respirable participles in a bulk material comprising particles. The method comprises: analyzing morphology of the particles; analyzing chemical composition of the particles; creating a profile of the particles, wherein each particle in the profile is characterized by its shape, size and chemical composition; selecting particles from the profile which match the size and chemical composition of a respirable particle; and calculating a percentage of the respirable particles in the bulk material. 1. A method for detecting respirable participles in a bulk material comprising particles , the method comprising:analyzing morphology of the particles;analyzing chemical composition of the particles;creating a profile of the particles, wherein each particle in the profile is characterized by its shape, size and chemical composition;selecting particles from the profile which match the size and chemical composition of a respirable particle; andcalculating a percentage of the respirable particles in the bulk material.2. The method of claim 1 , wherein the morphology and chemical composition of the particles are analyzed by a scanning electron microscope interfaced with an energy dispersive X-ray spectrometer.3. The method of claim 1 , wherein the method comprises a step of resuspending the particles of the bulk material in a medium selected from water and/or organic solvent claim 1 , and filtering the suspension through a filter with a nominal pore size sufficiently small to retain the particles in the respirable size range.4. The method of claim 1 , wherein the particles are retained on a filter claim 1 , and wherein the morphology and chemical composition of the particles are analyzed by a scanning electron microscope interfaced with an energy dispersive X-ray spectrometer.5. The method of claim 1 , wherein the bulk material is a mixture of inorganic compounds.6. The method of claim 1 , wherein the respirable particles are smaller than 20 ...

METHOD AND APPARATUS FOR THE EXTRACTION OF VITAMIN D METABOLITES FROM HUMAN PLASMA

This invention relates to a method and apparatus or kit for extracting major metabolites of vitamin D from human plasma or serum. More particularly, the invention provides for the extraction from human plasma or serum samples comprising vitamin D metabolites such as 1,25-dihydroxy vitamin D3, 25-hydroxy vitamin D2, and D3 from protein binding, removal of protein and phospholipids, and isolation of the metabolites using a combination of ion-exchange and Lewis acid mechanisms without the requirement to acidify the samples. The method and apparatus of the invention comprise a cartridge or plurality of cartridges comprising at least one protein crash frit, a strong cation exchanged sorbent, and an acidified alumina sorbent to provide higher recoveries of vitamin D metabolites than existing phospholipid depletion plate techniques. Accurately quantifying 1,25-dihydroxy vitamin D3 is useful in differential diagnosis of vitamin D-related diseases and for monitoring vitamin D therapy in patients with chronic renal disease. 1. An apparatus for isolating metabolites of vitamin D or small molecules from a non-acidized serum or plasma sample comprising proteins , metabolites of vitamin D , small molecules , phospholipids and interfering compounds in an aqueous media , said apparatus comprising:at least one well having a generally tubular side wall defining an interior well space having an upper inlet and a lower outlet;a first protein crash frit disposed in the interior well space between the upper inlet and the lower outlet defining an introduction zone above the first protein crash frit and between the upper inlet and the first protein crash frit;a first sorbent zone comprising a cation exchanged silica sorbent disposed adjacent to and below the first protein crash frit;a second protein crash frit disposed in the interior well space between the first sorbent zone and the second sorbent zonea second sorbent zone disposed below said first adsorbent zone comprising an acidized ...

Portable multimode reverse osmosis water purification system

A water purification system is disclosed which, includes a reverse osmosis (RO) system or component that is connectable to a city or other outside water feed that is capable of responding to and compensating for low or no feed water pressure coming into the RO system to ensure the outgoing supply of purified water is provided consistently and at a minimum water pressure. This can be accomplished without the need for communication with another device or system-wide facility, such as a hospital, or a pharmaceutical or semiconductor manufacturing system, requiring a constant water supply.

USE OF HOLLOW FIBERS TO OBTAIN BLOOD OR A BLOOD DERIVATIVE IMPOVERISHED FROM BLOOD CELLS AND PLATELETS DERIVED EXTRACELLULAR VESICLES

A method is provided for using hollow fibers having a porosity above 20 nm, in particular polyethersulfone hollow fibers, to impoverish blood and blood-derivatives from blood-derived extracellular vesicles, in particular exosomes and exomers. Methods for obtaining and analyzing the impoverished samples are also provided. 1. A method for using hollow fibers , the method comprising impoverishing , using hollow fibers having a porosity above 20 nm , by gravity filtering , a blood or blood-derived liquid sample from extracellular vesicles , the extracellular vesicles having a size that is inferior to the porosity of the hollow fibers and originating from blood components selected from the group consisting of blood red cells , blood white cells , platelets , and combinations thereof.2. The method of claim 1 , wherein the hollow fibers comprise polyethersulfone hollow fibers.3. The method of claim 1 , wherein the hollow fibers are polyethersulfone hollow fibers.4. The method of claim 1 , wherein the extracellular vesicles are selected from the group consisting of microvesicles claim 1 , exosomes claim 1 , exomers claim 1 , and combinations thereof.5. The method of claim 1 , wherein the porosity of the hollow fibers is of about 200 nm.6. The method of claim 1 , wherein the porosity of the hollow fibers is comprised between about 150 and about 500 nm.7. The method of claim 1 , wherein the blood-derived liquid sample is a plasma sample.8. A method for obtaining blood or a blood derivative impoverished in extracellular vesicles originating from blood components selected from the group consisting of red blood cells claim 1 , white blood cells claim 1 , platelets claim 1 , and combination thereof claim 1 , the method comprising gravity filtering a blood or blood-derived liquid sample through a filter comprising hollow fibers having a porosity above 20 nm claim 1 , thereby obtaining blood or a blood derivative impoverished in extracellular vesicles claim 1 , the extracellular ...

METHOD, SYSTEM AND APPARATUS FOR BLOOD PROCESSING UNIT

The disclosed embodiments may be used, among others, to extract particles from blood. The particles may include pathogens, viruses, bacteria and other microorganisms present in mammalian blood. An embodiment of the disclosure relates to a system to detect one or more blood-borne pathogens. The exemplary system includes: a transfer assembly having a tube and a hallow needle, the hallow needle centrally located within the transfer assembly tube and configured to communicate a sample material therethrough; a lysing syringe to couple to the transfer assembly, the lysing syringe comprising one or more lysing reagent and a plunger activatable to receive the sample material through the transfer assembly; and a large volume concentrator (LVC) to sealingly couple to the lysing syringe and to separate at least one pathogen from the sample material, the LVC further comprising: a filter support, a membrane, a retainer and a threaded portion. 1. A system to detect one or more blood-borne pathogens , the system comprising:a transfer assembly having a tube and a hallow needle, the hallow needle centrally located within the transfer assembly tube and configured to communicate a sample material therethrough;a lysing syringe to couple to the transfer assembly, the lysing syringe comprising one or more lysing reagent and a plunger activatable to receive the sample material through the transfer assembly; anda large volume concentrator (LVC) to sealingly couple to the lysing syringe and to separate at least one pathogen from the sample material, the LVC further comprising: a filter support, a membrane, a retainer and a threaded portion;wherein the retainer is configured to secure the membrane against the filter support.2. The system of claim 1 , further comprising an adapter to couple to the LVC claim 1 , the adapter configured to allow a twisting motion along a longitudinal axis thereof.3. The system of claim 1 , further comprising an agitator to receive the lysing syringe containing ...

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to transfer the blood sample to a point-of-care testing device or a biological fluid separation and testing device. After transferring the blood sample, the biological fluid separation and testing device is able to separate the plasma portion from the cellular portion and analyze the blood sample and obtain test results. 1. A biological fluid separation device , comprising:a rotatable body having a center of rotation, an outer periphery, and a body inlet, the body inlet adapted to receive a multi-component blood sample;a separation chamber defined within the rotatable body and in fluid communication with the body inlet and having a chamber outlet spaced apart from the body inlet, the separation chamber adapted to receive the multi-component blood sample; anda blood component chamber defined within the rotatable body and in fluid communication with the chamber outlet,wherein when the separation chamber contains the multi-component blood sample and a rotational force is applied to the rotatable body, a first blood component of the multi-component blood sample passes from the separation chamber into the blood component chamber and a second blood component of the multi-component blood sample is retained within the separation chamber,wherein the blood component chamber is disposed adjacent the center of rotation and the separation chamber is disposed adjacent the outer periphery of the rotatable body, andwherein the body inlet includes an engagement portion configured to receive a transfer device for closed transfer of a multi-component blood sample to the separation device.2. The biological fluid separation device of claim 1 , wherein the first blood component is a plasma component of the multi-component blood sample and the second blood component is a ...

Biological Fluid Separation Device and Biological Fluid Separation and Testing System

A biological fluid separation device that is adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid separation device adapted to receive a multi-component blood sample , the biological fluid separation device comprising:a separation cartridge having an inlet port and a flow channel defined within the cartridge in fluid communication with the inlet port, the flow channel containing a separation element adapted to separate the multi-component blood sample into at least a first component and a second component, a first collection chamber defined within the cartridge in fluid communication with the flow channel and including a first outlet port, and a second collection chamber defined within the cartridge in fluid communication with the flow channel and including a second outlet port, the second collection chamber isolated from the first collection chamber.2. The biological fluid separation device of claim 1 , wherein the first component is a cellular portion of the multi-component blood sample and the second component is a plasma portion of the multi-component blood sample.3. The biological fluid separation ...

Biological Fluid Separation Device and Biological Fluid Separation and Testing System

A biological fluid separation device that is adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid separation device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid separation device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid separation device of the present disclosure also provides a closed separation and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid separation device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid separation device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid separation cartridge , comprising:a housing having an inlet port and a flow channel defined within the housing in fluid communication with the inlet port;a first collection chamber defined within the housing in fluid communication with the flow channel and including a first outlet port having a valve comprising a deformable wall member and a septum, wherein the septum covers the first outlet port; anda second collection chamber defined within the housing in fluid communication with the flow channel and including a second outlet port, the second collection chamber isolated from the first collection chamber, and the second outlet port spaced apart from the first outlet port.2. The biological fluid separation cartridge of claim 1 , wherein the flow channel has a spiral shape.3. The biological fluid separation cartridge of claim 1 , wherein at least a portion of the flow channel contains a sample stabilizer.4. The biological fluid separation cartridge of claim 1 , further comprising an inlet channel in fluid ...

METHOD AND ASSOCIATED DEVICE FOR RAPID DETECTION OF TARGET BIOMOLECULES WITH ENHANCED SENSITIVITY

A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided. 1. A method for rapid diagnostic testing of a source biological sample comprising tuberculosis-lipoarabinomannan (TB-LAM) , comprising: diluting the source biological sample by at least 2× compared to the source urine sample to form a diluted biological sample;', 'contacting the diluted biological sample to an anion-exchange medium to capture the TB-LAM of the diluted biological sample;', 'capturing the TB-LAM of the diluted biological sample by the anion-exchange medium; and', 'eluting the captured-TB-LAM from the anion-exchange medium as a concentrated form of TB-LAM in an eluate under a salt concentration of at least 1M, wherein a concentration of the TB-LAM in the eluate is in a range from about 2× to 25× compared to a concentration of the TB-LAM in the diluted biological sample; and, 'concentrating the TB-LAM byloading the eluate comprising the concentrated form of the TB-LAM to a rapid diagnostic testing device comprising a TB-LAM-specific antibody for binding the concentrated form of the TB-LAM,wherein the eluate is loaded without any dilution, and wherein the TB-LAM binds to the TB-LAM-specific ...

MEMBRANE FOR SEPARATION OF STEM CELLS FROM BIOLOGICAL SAMPLES, PRODUCTION PROCESS FOR SAID MEMBRANE, AND PROCESS AND DEVICE FOR SEPARATION, COMPRISING SAID MEMBRANE

The subject of the invention is a membrane for separation of target stem cells from biological samples, more precisely from a single-cell suspension that was prepared from a biological sample. As a result, sterile target stem cells are obtained in physiological buffer. The membrane of the invention consists of a 3D carrier structure made of at least one layer of biocompatible polymer with specific pore size, as a carrier material, and covalently bound target molecules on its surface and/or in the pores. These target molecules are preferably target antibodies, which recognize characteristic antigens that are bound on the surface of the target stem cells and thus bind the target stem cells to the membrane. Target molecules can be either directly bound to the surface and/or in the pores of the carrier structure or are bound to the surface and/or in the pores of the carrier structure through specific functionalized nanoparticles, which are bound to or embedded into the 3D carrier structure of the membrane. In addition, the present invention includes the membrane production process as well as the process and device for the separation of target stem cells from a biological sample, which includes the above membrane as a constituent part. 1. A membrane for separation of target stem cells from a single-cell suspension containing stem cells , whereby said single-cell suspension is obtained from a biological sample containing stem cells , wherein the membrane consists of a 3D carrier structure with integrated target molecules on the surface and/or in the pores of said carrier structure , whereby said carrier structure is made of at least one layer of a biocompatible polymer with pores with a diameter in the range from 50 to 500 μm and wherein said carrier structure has on its surface and/or in the pores covalently bound target molecules which recognize and bind characteristic antigens on the surface of target stem cells.2. The membrane according to claim 1 , wherein each ...

DEVICE AND METHOD FOR FILTERING BLOOD

The invention relates to a device and a method for filtering a liquid sample, wherein first capillary-driven filtration occurs and, after initial filling, pressure-operated filtration is performed by applying a vacuum or a positive pressure. 112. A device () for filtration of a liquid sample () ,{'b': 3', '2, 'having a membrane () for filtering the sample (), and'}{'b': 4', '5', '2, 'having a carrier () that comprises or forms a fluidic system () for receiving the filtered sample (),'}characterized in that{'b': 1', '15', '2', '5, 'the device () has an associated conveying device () which generates a negative or positive pressure for further filtration or acceleration of the filtration only after the filtered sample () has reached or partly filled the fluidic system () as a result of capillary forces, or'}{'b': 1', '11', '2', '3, 'the device () comprises a feed device () for the sample (), in capillary contact with the membrane ().'}25832. The device according to claim 1 , characterized in that the fluidic system () comprises a receiving opening () arranged at least substantially centrally under the membrane () for receiving and draining off the filtered sample ().3839439. The device according to claim 2 , characterized in that the receiving opening () opens towards the membrane () in an elevated region () of the carrier () claim 2 , the membrane () preferably lying on the region ().496436142. The device according to claim 3 , characterized in that the region () is surrounded claim 3 , particularly in an annular configuration claim 3 , by a cover () of the carrier () claim 3 , the membrane () preferably resting loosely on the cover () claim 3 , optionally together with a receiving device () for the sample ().587434193. The device according to claim 2 , characterized in that adjoining the receiving opening () is a receiving channel () which is formed by an opening in the carrier () claim 2 , the membrane () being directly connected to the carrier () at the edges and/ ...

METHODS AND APPARATUS TO SELECTIVELY EXTRACT CONSTITUENTS FROM BIOLOGICAL SAMPLES

Methods and apparatus provide filtration for concentrating analytes, such as bacteria or exosomes, of a biological sample, such as blood or urine. The technology may employ membrane devices that implement one or more tangential flow filtration processes such as in stages. An example membrane device may typically include a membrane having sides and ends. The membrane may selectively permit constituent(s) of the sample to pass through while retaining other constituents at one side. An input chamber of the device may include an inlet near one end and an outlet near the other end, and that may permit a tangential flow of the sample along the first side surface, and a trans-membrane passing of constituent(s). An output chamber of the device may be configured at the second side surface to receive the passing constituents. Such devices may be provided in a kit to facilitate targeting of a desired biological analyte concentration. 1. Apparatus for extracting constituents from a biological sample for concentrating a desired constituent of the biological sample , the apparatus comprising: a membrane having a first side, a second side, a first end and a second end, the membrane having a characteristic to selectively permit one or more constituents of the biological sample to pass through the membrane from the first side to the second side while retaining other constituents of the biological sample at the first side;', 'an input chamber having an inlet proximate to the first end and an outlet proximate to the second end, the input chamber configured at the first side of the membrane to permit (a) a tangential flow of the biological sample along a first surface of the membrane at the first side from the inlet to the outlet, and (b) a trans-membrane passing of the one or more constituents of the biological sample from the first side to the second side; and', 'an output chamber configured at a second surface of the membrane at the second side and configured to receive the one or ...

POLYMER MICROFILTERS, DEVICES COMPRISING THE SAME, METHODS OF MANUFACTURING THE SAME, AND USES THEREOF

A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells. 1. A microfilter comprising:a first polymer layer formed from an epoxy-based photo-definable dry film, wherein the first polymer layer has flexibility to be disposed on a roll and unrolled;a plurality of first apertures each extending through the first polymer layer formed by exposing the first polymer layer to a UV light via an optical mask to obtain a selected shape of said apertures based on said optical mask, said first polymer layer forming at least a portion of said microfilter; anda surface on said first polymer layer modified to be hydrophilic,wherein said surface comprises bonding sites for reagents.2. The microfilter of further comprising a second polymer layer formed from photo-definable dry film and having second apertures extending through the second polymer layer claim 1 , wherein the second polymer layer has flexibility to be disposed on a roll and unrolled claim 1 , and at least one of the first apertures and at least one of the second apertures define at least a portion of a passage extending through the first and second layers.3. The microfilter of claim 1 , wherein said surface on said first polymer layer is modified to include a rough nanosurface.4. The microfilter of claim 1 , wherein the surface is modified by changing of a surface energy has claim 1 , the polymer layer having the surface energy raised.5. The microfilter of claim 1 , wherein said first polymer layer has a uniform thickness of 5 to 100 microns.6. The microfilter of claim 1 , wherein said first polymer ...

Cell collecting device

A cell collecting device is configured to collect target cells from a fluid sample such as blood or physiological fluid. The cell collecting device includes a conduit through which a fluid sample containing target cells passes. The cell collecting device further includes a screen filter installed inside the conduit and configured to guide the target cells toward a specified region. The screen filter includes a plurality of filtering holes for filtering the target cells.

ADDING ION SENSITIVITY TO AN INTEGRATED COMPUTATIONAL ELEMENT (ICE)

A device including an ion-selective membrane arranged within an optical path of the device and coupled to a sample cell to interact with a fluid sample and thereby modify an optical response of the ion-selective membrane according to an ion concentration in the fluid sample, is provided. The device also includes an integrated computational element (ICE) arranged within the optical path, so that the illumination light optically interacts with the ICE and with the ion-selective membrane to provide a modified light that has a property indicative of the ion concentration in the fluid sample. A detector that receives the modified light provides an electrical signal proportional to the property of the modified light. A method and a system for using the above device are also provided. 1. A device , comprising:an ion-selective membrane arranged within an optical path of the device and coupled to a sample cell to interact with a fluid sample and thereby modify an optical response of the ion-selective membrane according to an ion concentration in the fluid sample, wherein the optical path of the device is defined by an illumination light;an integrated computational element (ICE) arranged within the optical path, wherein the illumination light optically interacts with the ICE and with the ion-selective membrane to provide a modified light that has a property indicative of the ion concentration in the fluid sample; anda detector that receives the modified light and provides an electrical signal proportional to the property of the modified light.2. The device of wherein the sample cell facilitates optical interaction between the illumination light and the ion-selective membrane to generate a sample light claim 1 , and the sample light interacts with the ICE to provide the modified light.3. The device of claim 1 , wherein the ion-selective membrane absorbs a pre-selected type of ions from the fluid sample up to an equilibrium value determined by the ion concentration in the ...

METHOD AND KIT FOR SAMPLE PREPARATION AND ENDOTOXIN DETERMINATION

The invention relates to a method for preparation of a sample () of a formulation () for subsequent endotoxin determination, the formulation () suspected of comprising an endotoxin, the formulation () preferentially being a pharmaceutical formulation. The method comprises the following steps: application of the sample () to an endotoxin-free centrifugation column () containing a size exclusion chromatography matrix () that has been equilibrated with a suitable equilibration buffer () and elution of a flow through () of the sample by centrifugation, which flow through () can then be used for endotoxin determination. The equilibration buffer () is selected according to a subsequently used method of endotoxin determination, the equilibration buffer () only containing components not interfering with subsequently used method of endotoxin determination. Furthermore, the invention relates to a kit () for preparation of a sample (). 16. A method for evaluation of Low Endotoxin Recovery (LER) effects in a pharmaceutical formulation and/or for an elaboration of a composition of a suitable equilibration buffer () , comprising:spiking an undiluted sample of a pharmaceutical formulation with a known activity of a lipopolysaccharide (LPS) standard,testing an aliquot of the spiked sample for endotoxin,{'b': 2', '2', '5', '2', '6, 'if the spiked sample shows a test result corresponding to less than 50% of the known activity of the lipopolysaccharide (LPS) standard spiked into the sample, then applying aliquots of the spiked sample to at least two endotoxin-free centrifugation columns (), wherein the centrifugation columns () containing a size exclusion chromatography matrix () and each of the at least two endotoxin-free centrifugation columns () having been equilibrated with different equilibration buffers ();'}{'b': 15', '2', '15, 'eluting a flow through () from each centrifugation columns () by centrifugation, which flow through () can then be used for endotoxin determination; ...

IN VITRO INTESTINAL DRUG DISPOSITION DEVICE

An in vitro intestinal drug disposition device () comprises a donor chamber () for a donor solution and having a bottom end () and a top end (). The device () also comprises a receiver chamber () for an absorption solution and an absorption membrane () arranged in between and separating the chambers (). A first side () of the absorption membrane () is to be in contact with the donor solution and a second side () of the absorption membrane () is to be in contact with the absorption solution. A ratio of an internal volume of the donor chamber () to an area of the first membrane side () is equal to or smaller than 3 ml/cm. A cross-sectional area of the donor chamber () at the bottom end () is larger than a cross-sectional area of the donor chamber () at the top end (). 110.-. (canceled)11. An in vitro intestinal drug disposition device comprising:a donor chamber configured to comprise a donor solution and having a bottom end and a top end;a receiver chamber configured to comprise an absorption solution; andan absorption membrane arranged in between and separating said donor chamber and said receiver chamber, whereina first main side of said absorption membrane is configured to be in contact with said donor solution and a second, opposite main side of said absorption membrane is configured to be in contact with said absorption solution;{'sup': '2', 'a ratio of an internal volume of said donor chamber to an area of said first main side of said absorption membrane is equal to or smaller than 3 ml/cm; and'}a cross-sectional area of said donor chamber at said bottom end is larger than a cross-sectional area of said donor chamber at said top end.12. The in vitro intestinal drug disposition device according to claim 11 , wherein said ratio of said internal volume of said donor chamber to said area of said first main side of said absorption membrane is equal to or smaller than 2.5 ml/cm.13. The in vitro intestinal drug disposition device according to claim 12 , wherein said ...

NON-DESTRUCTIVE SAMPLING DEVICE FOR EXTRACTION OF A MARKER

Method and device for extraction of markers, including: introducing a sample into a removable support basket placed in the housing of a cell of an extraction device provided with a flexible membrane and immersing the tissue sample in a liquid previously introduced into the housing, followed by hermetic closing of the extraction cell and application of a pressure cycle on the flexible membrane for a period of 1 to 5 minutes at a frequency between 1 and 2 Hz. Then, total or partial recovery of the liquid immersing the sample after the pressure cycle and detection of the markers present in the liquid. 1. A method for extracting a marker , comprising:introducing a sample into a removable support basket placed in the housing of a cell of an extraction device provided with a flexible membrane andimmersing the sample in a liquid previously introduced into the housing;hermetically closing the extraction cell after immersion of the sample in the liquid andapplying a pressure cycle on the flexible membrane for a period of 1 to 5 minutes, at a frequency of between 1 and 2 Hz;totally or partially recovering the liquid immersing the sample after the pressure cycle, and detecting the markers present in the physiological liquid;wherein the pressure applied on the membrane is between 2 and 5 bar.2. The method as claimed in claim 1 , wherein the duration of the pressure cycle is 3 minutes.3. The method as claimed in claim 1 , wherein the sample is a biological tissue sample and the liquid is a physiological liquid for the extraction of biomarkers.4. The method as claimed in claim 3 , wherein the biological tissue sample is extracted from a cancerous tumor.5. The method as claimed in claim 3 , wherein the physiological liquid is a PBS buffer with 9 g/l NaCl.6. A device for extracting a marker claim 3 , comprising:an extraction cell provided with a housing configured to receive a removable support basket, said basket being configured to immerse a sample in a liquid previously ...

Polymeric Whole Blood Hollow Fiber Membrane Filter Medium and Use Thereof For Separating Blood Plasma/Serum From Whole Blood

A whole blood hollow fiber membrane filter medium is made of a polymeric material having pores of a pore size that ensures permeability to blood plasma or serum but retains blood cells. The whole blood hollow fiber membrane filter medium is used for filtering a whole blood sample so that blood plasma or serum passes through the whole blood hollow fiber membrane filter medium and blood cells are retained. The obtained blood plasma shows no hemolysis.

APPARATUS FOR PROCESSING BIOLOGICAL SAMPLE

An apparatus for processing biological sample is provided, which comprises a semi-permeable membrane column, an adaptor column and a vacuum manifold. The at least one semi-permeable membrane column is configured for accommodating biological sample therein and comprises a curved surface. The at least one adaptor column is configured for accommodating the semi-permeable membrane column therein and comprises a stopping element. The vacuum manifold is configured for creating a vacuum that facilitates flowing of the biological sample into the semi-permeable membrane column through a semi-permeable membrane therein. The stopping element abuts against the curved surface. By disposing the stopping element within the adaptor column, the present disclosure prevents the biological sample from suffering an unbalance pressure to splash upwardly outside the semi-permeable membrane column thereby causing a possibility of decreasing the testing efficiency, when the semi-permeable membrane column is removed out of the adaptor column. 1. An apparatus for processing biological sample , comprising:at least one semi-permeable membrane column wherein a first receiving space is defined in an inner portion of the semi-permeable membrane column, a bottom of the first receiving space comprises at least one semi-permeable membrane, a top portion of the semi-permeable membrane column comprises a first opening and a first protrusion protruding radially outward, and a bottom portion of the semi-permeable membrane column comprises a first outlet and a curved surface;at least one adaptor column wherein a second receiving space is defined in an inner portion of the adaptor column, a top portion of the adaptor column comprises a second opening and a second protrusion protruding radially outward, and a bottom portion of the adaptor column comprises a second outlet and a stopping element, a diameter of the second receiving space is slightly larger than an outer diameter of the first receiving space, ...

SAMPLE PREPARATION DEVICE

A manually actuated chromatography device comprising a chamber for receiving a liquid sample, a pump with a metering valve, and a chromatography element, wherein the pump moves a predetermined volume of liquid from the sample chamber to the chromatography element. 1. A manually actuated chromatography device comprising a chamber for receiving a liquid sample , a pump with a metering valve , and a chromatography element , wherein the pump moves a predetermined volume of liquid from the sample chamber to the chromatography element.2. The device according to wherein the chromatography element is a size exclusion chromatography element.3. The device according to wherein the device comprises a first part and a separate second part receivable in the first part claim 1 , preferably wherein the pump is actuated by the second part of the device operably engaging the first part of the device.4. The device according to wherein the metering valve is actuated by the second part of the device operably engaging the first part of the device.5. The device according to wherein the pump moves a predetermined volume of liquid from the sample chamber through the size-exclusion chromatography element.6. The device according to wherein the pump moves a predetermined volume of liquid from the sample chamber through the size-exclusion chromatography element to a sample collection vessel.7. The device according to wherein the metering valve comprises a metering chamber with an upper portion and a lower portion separated by a movable metering member.8. The device according to wherein the upper portion and lower portion of the metering chamber are selectively in fluid communication.9. The device according to wherein the metering valve comprises a pressure release channel for providing fluid communication between the upper and lower portions of the metering chamber.10. The device according to wherein the pump is pneumatic.11. The device according to wherein the predetermined volume of fluid is ...

AUTOMATED SYSTEM FOR REMOTE INLINE CONCENTRATION OF ULTRA-LOW CONCENTRATIONS IN PURE CHEMICALS

Systems and methods are described to concentrate a remote sample for analysis. A sample concentration system embodiment includes, but is not limited to, a plurality of valves including at least a first valve, a second valve, and a third valve; a plurality of columns including at least a first column and a second column, the first column fluidically coupled to the first valve, the second column fluidically coupled to the second valve; and a flow meter coupled with the third valve, the flow meter fluidically coupled with each of the first column and the second column when the plurality of valves is in a first flow path configuration to measure an amount of the liquid sample passed through the first column and the second column, wherein the plurality of valves includes a second flow path configuration and a third flow path configuration. 1. A sample concentration system for analysis of a liquid sample by an analysis system , comprising:a plurality of valves including at least a first valve, a second valve, and a third valve, the plurality of valves switchable between differing flow path configurations;a plurality of columns including at least a first column and a second column, the first column and the second column configured to retain at least one chemical of interest from a liquid sample, the first column fluidically coupled to the first valve, the second column fluidically coupled to the second valve; anda flow meter coupled with the third valve, the flow meter fluidically coupled with each of the first column and the second column when the plurality of valves is in a first flow path configuration to measure an amount of the liquid sample passed through the first column and the second column, wherein the plurality of valves includes a second flow path configuration in which the first column, the first valve, the second valve, and the third valve are in fluid communication and the second column and the flow meter are not in fluid communication with the first column, ...

Whole Blood Analytic Device And Method Therefor

Devices and methods are presented in which a plasma separation device with a first and second portion separates a blood containing fluid. Most preferably, the first portion produces a cell fraction and a plasma fraction, and the second portion captures the plasma fraction. A first actuator then fluidly isolates a portion of the plasma fraction within the second portion, and a second actuator moves the isolated portion of the plasma fraction from the second portion. 1. An analytic device , comprising:a sample receiving compartment;a plasma separation device that is at least partially disposed in the sample receiving compartment and comprises (1) a first portion configured to separate blood into a cell fraction and a plasma fraction, and (2) a second portion configured to capture at least a portion of the plasma fraction;wherein the analytic device has a first area that, when compressed by a first actuator, restricts flow of the plasma fraction between the first and the second portion; andwherein the analytic device has a second area that, when compressed by a second actuator, causes flow of a metered volume of the plasma fraction into at least one of the sample receiving compartment and a second compartment.2. The device of claim 1 , wherein the first portion comprises a hydrophobic separation membrane.3. The device of claim 1 , wherein the second portion comprises a hydrophilic membrane having a void volume for collection of a predetermined volume of plasma.4. The device of claim 1 , wherein the first and second portions are fluidly coupled to each other to allow capillary flow of the plasma fraction from the first portion to the second portion.5. The device of claim 1 , wherein the first area is formed from a flexible wall of the analytic device.6. The device of claim 1 , wherein the first actuator is configured as a knife edge.7. The device of claim 1 , wherein the second area is formed from a flexible wall of the analytic device.8. The device of claim 1 , wherein ...

Chemical sensor and method for detecting target substance

A chemical sensor for detecting a target substance in a gas sample, including a membrane; and an olfactory receptor fragment which is fixed to the membrane. The chemical sensor optionally includes a source electrode connected to one end of the membrane; and a drain electrode connected to the other end of the membrane, in which the membrane is a graphene.

ISOLATION DEVICE AND ISOLATION METHOD FOR ISOLATING TARGET PARTICLES FROM LIQUID SAMPLES

An isolation device for isolation of target particles from a plurality of liquid samples includes a plurality of isolation chips and a vacuum system. Each of the plurality of isolation chips includes a sample reservoir, and a first outlet and a second outlet disposed at opposite sides of the sample reservoir. The vacuum system includes a first vacuum pump connected to the first outlet of each of the plurality of isolation chips and a second vacuum pump connected to the second outlet of each of the plurality of isolation chips. The first vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding first outlet. The second vacuum pump generates a negative pressure in each of the plurality of isolation chips through a corresponding second outlet. The target particles are isolated from each of the plurality of liquid samples in a corresponding sample reservoir.

DETECTION OF ANALYTES BY NANOPORE WITHOUT USING ELECTRODES

A system without electrodes for identifying analytes based on optical measurement of ion flux through nanopores that is driven by a chemical gradient, and a method for identifying an analyte by using such system. A nanopore array without electrodes for identifying different analytes in parallel, a method for identifying different analytes by using such array, and a method of manufacturing such array. 1. A system without electrodes for identifying an analyte , the system comprising:(a) a first compartment having a first aqueous solution in it, wherein the first aqueous solution comprises a fluorescent reporter molecule capable of emitting fluorescence when bound to an ionic species;(b) a second compartment having a second aqueous solution in it, wherein the second aqueous solution comprises the ionic species which specifically bound to said fluorescence reporter molecule; and(c) a membrane separating the first compartment and second compartment; wherein the membrane between the first compartment and the second compartment has at least one inserted nanopore such that the first compartment and the second compartment are connected by the nanopore;wherein there is a chemical gradient of the ionic species between the first compartment and the second compartment which can drive the ionic species to diffuse from the second compartment to the first compartment through the nanopore.2. (canceled)3. The system according to claim 1 , wherein the membrane is a semipermeable membrane consisting of lipids or triblock copolymers.4. The system according to claim 3 , wherein the osmolarity of the second aqueous solution is higher than the osmolarity of the first aqueous solution or the osmolality of the second aqueous solution is higher than the osmolality of the first aqueous solution; or the osmolarity of the second aqueous solution is equal to the osmolarity of the first aqueous solution or the osmolality of the second aqueous solution is equal to the osmolality of the first ...

MICROFLUIDIC SEPARATION FROM WATER AND DIRECT OPTICAL DETECTION OF CHLORINE

A microfluidic device includes a sample inlet for a fluid sample, a degassing chamber having a gas-permeable membrane and defining first and second portions separated by the gas permeable membrane, and a detection chamber. The sample inlet and the detection chamber are fluidly coupled to the first and second portions, respectively of the degassing chamber. The detection chamber is operably coupled to a light source and a detector. Assessing a concentration of chlorine gas in an aqueous sample includes providing an aqueous sample to a microfluidic device, separating gas from the aqueous sample in the microfluidic device, providing the gas to a detector, assessing the absorbance of the gas sample at a known absorption wavelength of chlorine, and based on the assessed absorbance of the gas sample at the known absorption wavelength of chlorine, assessing a concentration of chlorine gas in the aqueous sample. 1. A microfluidic device comprising:a sample inlet for a fluid sample;a degassing chamber comprising a gas-permeable membrane and defining a first portion and a second portion separated by the gas permeable membrane;a light source;a detection chamber; anda detector;wherein the sample inlet is fluidly coupled to the first portion of the degassing chamber, the detection chamber is fluidly coupled to the second portion of the degassing chamber, and the light source and the detector are operably coupled to the detection chamber.2. The microfluidic device of claim 1 , further comprising a gas inlet fluidly coupled to the first portion of the degassing chamber.3. The microfluidic device of claim 1 , wherein the light source comprises a light-emitting diode.4. The microfluidic device of claim 1 , wherein the detector comprises a differential optical absorption spectrometer.5. The microfluidic device of claim 1 , wherein the detector comprises a charge-coupled device detector array.6. The microfluidic device of claim 1 , further comprising a battery operatively coupled to ...

METHOD AND SYSTEM FOR MEASURING CONTENT OF DISSOLVED ORGANIC HALOGENS IN WATER

A method and system for measuring the content of dissolved organic halide in water. The method includes separating dissolved inorganic halides and dissolved organic halides in water using an electrodialysis technique (S), which may completely separate the dissolved organic halides and the dissolved inorganic halides, avoid the use of activated carbon and adsorption columns, simplify steps and improve efficiency. The method converts the separated dissolved organic halides into dissolved inorganic halides using a photocatalysis technique (S), which does not need a special high-temperature combustion device, active carbon and catalysts, and is simple and easy to be performed at room temperature in more environmentally friendly mode. The method also includes analyzing the converted dissolved inorganic halides using a device with an ion analysis function (S) to analyze and measure a total content index of the dissolved organic halides and an individual content index of each of the dissolved organic halides (fluorine, chlorine, bromine and iodine) in water to be measured. 1. A method for content measurement of dissolved organic halide in water , the method comprising:separating a dissolved inorganic halide and a dissolved organic halide in a water sample to be measured using an electrodialysis technique;converting the separated dissolved organic halide into a dissolved inorganic halide; andmeasuring a content of the dissolved organic halide in the water sample to be measured by analyzing the converted dissolved inorganic halide using an ion analysis instrument.2. The method of claim 1 , wherein the separating the dissolved inorganic halide and the dissolved organic halide in the water sample to be measured using the electrodialysis technique includes:providing an electrodialyser including a first concentration chamber, a dilution chamber, and a second concentration chamber arranged in order;injecting an inorganic electrolyte solution without halogens into the first ...

ASPIRATION-FREE WELL PLATE APPARATUS AND METHODS

A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir. 1. A method , comprising:disposing a test sample within a sample well of a well plate, the well plate including a top plate, a bottom plate defining a reservoir, and a membrane disposed between the top plate and the bottom plate, the test sample including a wash liquid and a sample material;centrifuging the test sample such that a centripetal acceleration associated with the centrifuging draws the sample material away from the membrane and opposes a binding of the sample material to the membrane,the membrane configured to filter the wash liquid from the test sample during the centrifuging such that the wash liquid can pass from the reservoir defined by the bottom plate in through a first portion of the membrane in a direction that directly opposes the centripetal acceleration during centrifuging, then in a second direction perpendicular to the first direction, then in a third direction opposite the first direction through a second portion of the membrane different than the first portion and is captured within a collection chamber in fluid communication with the reservoir via the membrane, such that wash liquid passes from the reservoir and through the membrane, while ...

Blood Sampling Transfer Device

A blood sampling transfer device that includes a lancing tape having a flow channel and a transfer cartridge removably connected to the lancing tape is disclosed. The blood sampling transfer device provides a closed system that reduces the exposure of a blood sample to both skin and environment and provides fast mixing of a blood sample with a sample stabilizer. 1. A blood sampling transfer device , comprising:an adhesive lancing tape having a target, and a flow channel in fluid communication with a portion of the target;a transfer cartridge removably connected to the lancing tape, the transfer cartridge having a reservoir, anda frangible portion integrally formed between the transfer cartridge and the lancing tape such that the transfer cartridge and the lancing tape are detachably connected to one another via the frangible portion,wherein the flow channel is configured to permit a blood sample to flow from the target and through the frangible portion before entering the transfer cartridge,wherein, in a first position when the transfer cartridge is connected to the lancing tape, the reservoir is configured to be in fluid communication with the flow channel, andwherein, in a second position when the transfer cartridge is disconnected from the lancing tape, the transfer cartridge is configured to be separated from the lancing tape.2. The blood sampling transfer device of claim 1 , wherein the target of the lancing tape is aligned with the flow channel to promote flow of the blood sample via capillary action.3. The blood sampling transfer device of claim 1 , wherein the target of the lancing tape is a circular graphic indicator.4. The blood sampling transfer device of claim 1 , wherein the transfer cartridge includes a transfer cartridge flow channel in fluid communication with the reservoir.5. The blood sampling transfer device of claim 4 , wherein the transfer cartridge flow channel comprises a sample stabilizer and the transfer cartridge flow channel is configured ...

Devices and Methods for Eluting and Concentrating Large DNA Molecules

This disclosure relates to mesofluidic devices and methods for eluting and concentrating a plurality of nucleic acid molecules. The mesofluidic device includes a device frame having a bottom surface upon which is defined a first reservoir and the second reservoir. The first reservoir includes a first electrode, and the second reservoir includes a second electrode. The first and second electrodes are configured for electrical connection. The mesofluidic device includes an elongated channel extending between the first reservoir and the second reservoir. The mesofluidic device includes a first slot having a first slot width. The first slot is configured to receive an insert. The first slot intersects the elongated channel. The mesofluidic device includes a second slot having a second slot width. The second slot is configured to receive a separation material having a first porosity. The second slot intersects the elongated channel. 1. A mesofluidic device for eluting and concentrating a plurality of nucleic acid molecules , the mesofluidic device comprising:a device frame having a bottom surface upon which is defined a first reservoir comprising a first electrode, a second reservoir comprising a second electrode, the first and second electrodes configured for electrical connection, and an elongated channel extending between the first reservoir and the second reservoir, the elongated channel having a channel width;a first slot having a first slot width, the first slot configured to receive an insert, wherein the first slot intersects the elongated channel; anda second slot having a second slot width, the second slot configured to receive a separation material having a first porosity wherein the second slot intersects the elongated channel,wherein the first slot width and the second slot with width are greater than the channel width.2. The mesofluidic device of claim 1 , wherein the plurality of nucleic acid molecules comprises deoxyribonucleic acid (DNA) molecules.3. The ...

Extracellular Vesicle Isolation by Nanomembranes

Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents. 1. A method for extracellular vesicle isolation comprising the steps of:(a) collecting a biofluid sample;(b) contacting the biofluid sample with a pre-filtration membrane, and forming a first filtrate and a first retentate;(c) optionally, washing the first retentate of the pre-filtration membrane;(d) contacting the first filtrate from the pre-filtration membrane with a first capture membrane and forming a second filtrate and a second retentate; and(e) optionally, washing the second retentate.2. The method of further comprising the step of:(f) eluting the second retentate from the first capture membrane to recover the contents of the second retentate.3. The method of further comprising the step of:(f) lysing the second retentate from the first capture membrane to isolate the contents of the second retentate.4. The method of further comprising the step of:(g) contacting the second filtrate with a second capture membrane thereby forming a third filtrate and a third retentate and eluting the third retentate from the second capture membrane.5. The method of further comprising the step of:(g) contacting the second filtrate with a second capture membrane thereby forming a third filtrate and a third retentate and eluting the third retentate from the second capture membrane.6. The method of wherein step ...

CELL SEPARATION CHIP AND METHOD FOR SEPARATING CELLS USING SAME

A cell separation chip for separating cells from a solution containing the cells includes an upper plate, a membrane positioned on a predetermined region of the upper plate, and an absorption device positioned at a lower end of the upper plate so as to absorb a solution which has penetrated the membrane from the solution containing the cells. There is an effect of effectively collecting/separating only cells in a solution by absorbing the solution containing the cells without the need of applying and maintaining additional external voltage. 1: A cell separation chip for separating cells from a solution containing the cells , comprising:an upper plate;a membrane positioned on a predetermined region of the upper plate; andan absorption device positioned at a lower end of the upper plate so as to absorb a solution which has penetrated the membrane from the solution containing the cells.2: The cell separation chip according to claim 1 , which further comprises a lower plate positioned at the lower end of the upper plate by being connected to the absorption device.3: The cell separation chip according to claim 1 , wherein the membrane comprises: an upper end region forming an upper end of the membrane as a region where the solution containing cells is contacted first; and a lower end region forming a lower end of the membrane as a region where the solution is contacted with the absorption device.4: The cell separation chip according to claim 3 , wherein the upper end region has a hole with a diameter of 2 μm or smaller.5: The cell separation chip according to claim 1 , wherein the lower end region has a hole with a diameter of 10 μm or greater.6: The cell separation chip according to claim 1 , wherein the upper plate is formed of a film or a plastic.7: The cell separation chip according to claim 2 , wherein the lower plate is formed of a film or a plastic.8: The cell separation chip according to claim 1 , wherein the absorption device is formed of a filter paper or a ...

SPERM SORTING APPARATUS AND SPERM SORTING METHOD

A sperm sorting apparatus and a sperm sorting method are provided. The sperm sorting apparatus includes a medium chamber, a waste chamber and a sorting channel communicated with and extending along a first direction in between. The medium chamber is configured to contain a medium solution. The waste chamber disposed aside the medium chamber is configured to contain a residual solution after sorting. The residual solution includes low motility sperms and/or dead sperms. The sorting channel is configured to be inserted with a sperm sample, such that sperms in the sperm sample are sorted in corresponding to a medium solution flow from the medium chamber. The sorting channel has first and second portions. The first portion is closer to the medium chamber than the second portion. A width of the first portion measured along a second direction is greater than a critical dimension, ranging from 200 μm to 400 μm. 1. A sperm sorting apparatus , comprising:a medium chamber, configured to contain a medium solution;a waste chamber, disposed aside the medium chamber, and configured to contain a residual solution obtained after sorting, wherein the residual solution includes low motility sperms and/or dead sperms; anda sorting channel, extending between and communicated with the medium chamber and the waste chamber along a first direction, and configured to be inserted with a sperm sample, such that sperms in the sperm sample are sorted in corresponding to a medium solution flow entering the sorting channel from the medium chamber, wherein the sorting channel has a first portion and a second portion, the first portion is closer to the medium chamber than the second portion, a width of the first portion measured along a second direction is greater than a critical dimension, the second direction is intersected with the first direction, and the critical dimension ranges from 200 μm to 400 μm.2. The sperm sorting apparatus according to claim 1 , further comprising parallel micro- ...

SILOXANE COLUMN BASED LIQUID PHASE MICROEXTRACTION AND PROCESS

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry. 1: A method for extracting an analyte in a sample comprising: a microwave extraction vial with a bottom portion and a headspace portion and', 'a porous membrane bag located in the headspace portion of the microwave extraction vial, said porous membrane bag encapsulating a liquid-phase extraction medium, wherein the sample and solution are disposed in the bottom portion, the porous membrane bag does not contact the sample and the solution, the porous membrane bag and the headspace portion are also subjected to the microwave-heating, and the microwave-heating and agitating produces a vapor in the headspace portion; and, 'microwave-heating and agitating the sample and a solution in a microwave extraction assembly comprisingextracting the analyte from the vapor to produce a vapor extract within the liquid-phase extraction medium; andfeeding the vapor extract to a gas chromatograph-mass spectrometer (GCMS) to detect and/or quantify the analyte, wherein the vapor extract passes through a methyl siloxane gas chromatographic column with a helium carrier gas.2. (canceled)3. (canceled)4: The method of claim 1 , wherein the weight of the sample relative to the volume of the liquid-phase extraction medium is 0.04-100 g/mL.5: The method of claim 1 , wherein the claim 1 , solution is an acid.6: The method of claim 5 , wherein the microwave-heating and agitating simultaneously digests the sample and extracts the analyte.7: The method of claim 5 , wherein the acid is at least one selected from the group consisting of nitric acid claim 5 , hydrochloric acid claim 5 , ...

PROCESS FOR MICROEXTRACTION AND DETECTION OF AN ANALYTE

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry. 1: A method for extracting an analyte in a sample comprising: a microwave extraction vial with a bottom portion and a headspace portion and', 'a porous polypropylene membrane bag located in the headspace portion of the microwave extraction vial, said porous membrane bag encapsulating a liquid-phase extraction medium comprising toluene,, 'microwave-heating and agitating the sample and a nitric acid solution in a microwave extraction assembly comprisingwherein the sample and the nitric acid solution are disposed in the bottom portion, the porous polypropylene membrane bag does not contact the sample and the nitric acid solution, the porous polypropylene membrane bag and the headspace portion are also subjected to the microwave-heating, and the microwave-heating and agitating produces a vapor in the headspace portion; andextracting the analyte from the vapor to produce a vapor extract within the liquid-phase extraction medium.2: The method of claim 1 , further comprising feeding the vapor extract to a gas chromatograph-mass spectrometer (GCMS) to detect and/or quantify the analyte.3. (canceled)4: The method of claim 1 , wherein the weight of the sample relative to the volume of the liquid-phase extraction medium is 0.04-100 g/mL.5. (canceled)6: The method of claim 5 , wherein the microwave-heating and agitating simultaneously digests the sample and extracts the analyte.7. (canceled)8. (canceled)9: The method of claim 1 , wherein the porous polypropylene membrane bag is positioned 1-10 cm above the sample.10: The method of claim 1 , wherein the weight of the sample ...

ASPIRATION-FREE WELL PLATE APPARATUS AND METHODS

A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir. 1. A method , comprising:disposing a test sample within a sample well of a well plate, the well plate including a top plate, a bottom plate and a membrane disposed between the top plate and the bottom plate, the test sample including a wash liquid and a sample material;centrifuging the test sample such that a centripetal acceleration associated with the centrifuging draws the sample material away from the membrane and opposes a binding of the sample material to the membrane,the membrane configured to filter the wash liquid from the test sample during the centrifuging such that the wash liquid can pass from a reservoir defined by the bottom plate in a direction that directly opposes the centripetal acceleration during centrifuging, and is captured within a collection chamber in fluid communication with the reservoir via the membrane, while the sample material remains within the reservoir.2. The method of claim 1 , wherein during the centrifuging claim 1 , prior to the wash liquid being captured within the collection chamber claim 1 , the wash liquid moves laterally in a plane of the membrane claim 1 , and then moves in a direction of the centripetal acceleration and ...

Molecular weight filtration system and apparatus

A molecular filtration device and method of use capable of filtering and purifying molecules of a particular characteristic, wherein the amount of molecule to be filtered may be in the nanogram range and may be dispersed in a relatively large volume of solution. The resultant elution may include a substantially pure solution comprising the desired molecule.

PASSIVE SAMPLER AND METHODS OF MAKING

Described herein are passive samplers, making of such samplers, and methods of use. In an example embodiment, a passive sampling membrane comprises, for example, a continuous mesoporous sequestration media having a sequestration phase and a support membrane configured to support the sequestration phase. The sequestration phase may include a hydrophobic region and a hydrophilic region. The continuous mesoporous sequestration media may be configured to simultaneously sequester polar and non-polar organic substances. 1. A passive sampling membrane , comprising:a continuous mesoporous sequestration media comprising a sequestration phase and a support membrane, the support membrane being configured to support the sequestration phase,wherein the sequestration phase comprises a hydrophobic region and a hydrophilic region,wherein the continuous mesoporous sequestration media is configured to simultaneously sequester polar and non-polar organic substances.2. The membrane of claim 1 , wherein the support membrane comprises a fiber sheet matrix.3. The membrane of claim 2 , wherein the fiber sheet matrix is selected from the group consisting of: cotton claim 2 , flax claim 2 , hemp claim 2 , ramie claim 2 , wool claim 2 , silk claim 2 , cellulose claim 2 , polyester claim 2 , polyimide claim 2 , polyurethane claim 2 , polyacrylic claim 2 , polytetrafluoroethylene (PTFE) claim 2 , polyvinylidene fluoride (PVDF) claim 2 , polyfluoroalkoxy (PFA) claim 2 , fluorinated ethylene propylene (FEP) claim 2 , glassfiber claim 2 , ceramic fiber claim 2 , quartz fiber claim 2 , carbon fiber claim 2 , and a combination thereof.4. The membrane of claim 1 , wherein the sequestration phase is a polymer.5. The membrane of claim 4 , wherein the polymer includes the hydrophobic region and the hydrophilic region claim 4 , wherein the hydrophobic region corresponds to a hydrophobic monomer and the hydrophilic region corresponds to a hydrophilic monomer.6. The membrane of claim 5 , wherein the ...

CHEMICAL SENSOR MODULE AND METHOD FOR IDENTIFYING SAMPLE SUBSTANCES

A chemical sensor module includes first to n-th (n is a natural number of 2 or greater) graphene sensors; and an exposure mechanism exposing the first to n-th graphene sensors to first to n-th aqueous solutions containing a sample substance and having different concentrations of phosphate ion, magnesium ion, or sulfate ion. The chemical sensor module identifies the sample substance from the difference in electrical characteristics of the first to n-th graphene sensors. 1. A chemical sensor module comprising:first to n-th (n is a natural number of 2 or greater) graphene sensors; andan exposure mechanism exposing the first to n-th graphene sensors to first to n-th aqueous solutions containing a sample substance and having different concentrations of phosphate ion, magnesium ion, or sulfate ion, andidentifying the sample substance from the difference in electrical characteristics of the first to n-th graphene sensors.2. The module according to claim 1 , further comprising: a mechanism mixing a gas containing the sample substance and an uptake solution, and', 'first to n-th mechanisms further mixing the uptake solution in which the sample substance is mixed with a liquid having a different concentration of phosphate ion, magnesium ion, or sulfate ion to obtain the first to n-th aqueous solutions., 'a mixing mechanism, which includes'}3. The module according to claim 1 , further comprising:a mixing mechanism including first to n-th mechanisms, the first to n-th mechanisms mixing a gas containing the sample substance with an aqueous solution having a different concentration of phosphate ion, magnesium ion, or sulfate ion to obtain the first to the n-th aqueous solutions.4. The module according to claim 1 , further comprising: a mechanism mixing a gas containing the sample substance and an uptake solution, and', 'a plurality of mechanisms mixing the first to (n−1)-th aqueous solutions exposed to the first to (n−1)-th graphene sensors, and a solution containing phosphate ...

LIQUID CONTAINING POLYMER MARKER, USE OF A POLYMER MARKER AS AUTHENTICATION TOOL, AND METHOD AND DEVICE FOR DETECTING A POLYMER MARKER DISSOLVED IN A LIQUID

The present invention relates to markers for liquids that can be used to authenticate the origin and genuineness of a liquid, preferably a bulk liquid such as fuel. For this purpose, the present invention teaches the use of a polymer capable of forming a semiconducting polymer particle (pdot) in the liquid in small amounts. The invention encompasses a liquid comprising a) a polymer that is capable of forming a semiconducting polymer particle (pdot), the concentration of the polymer being 10 ppm by weight or less, and b) an organic substance in an amount of 90% by weight or more. The invention furthermore envisages the use of such a polymer as an authenticating marker in a liquid, preferably a fuel, and a method for authenticating the genuineness and/or origin of a liquid comprising a polymer capable of forming a semiconducting polymer particle (pdot), comprising the steps 1. A method for authenticating the genuineness and/or origin of a liquid comprising a polymer capable of forming a semiconducting polymer particle (pdot) , comprising the stepsi. concentrating, isolating and/or extracting a polymer capable of forming a semiconducting polymer particle (pdot);ii. aggregating the polymer obtained in Step i. to form semiconducting polymer dots (pdots);iii. irradiating the formed pdots with electromagnetic radiation capable of exciting the pdots to emit electromagnetic radiation by fluorescence and/or phosphorescence, andiv. observing electromagnetic radiation emitted in response to the exciting irradiation of step iii.2. The method according to claim 1 , wherein the liquid is a fuel and wherein the concentration of the polymer capable of forming a semiconductor particle is equal to or less than 10 ppm by weight.3. The method according to claim 1 , wherein the method is implemented in a portable device.4. The method according to claim 1 , wherein the aggregation of the polymer to form pdots is performed by re-precipitation using water and a water-miscible organic ...

Medical Device for Collection of a Biological Sample

A biological fluid sampling device for collecting a blood sample from a separate vascular access device and for ejecting a portion of the collected sample to a point-of-care testing device for analysis is provided. The biological fluid sampling device includes a body enclosing a reservoir. The reservoir has an internal volume sufficient to contain enough blood for use in a diagnostic test. The sampling device further includes: an access lumen extending from a distal end of the body for establishing fluid communication between a separate vascular access device and the reservoir; an outflow lumen also in fluid communication with the reservoir; and a removable vented cap attached to the outflow lumen including a gas permeable vent in gaseous communication between the reservoir and ambient air. In addition, several sample and transfer devices are provided for obtaining a sample from a subject and transferring the sample to a point-of-care testing device. 1. A biological fluid sampling device engageable with a separate vascular access device , comprising:a housing enclosing a biological fluid reservoir;an access lumen in fluid communication with the separate vascular access device and the reservoir;an outflow lumen having a first end provided in fluid communication with the reservoir and a second end; and a gas permeable vent in gaseous communication between the reservoir and an ambient environment; and', 'a channel in fluid communication with the outflow lumen and positioned between the vent and the reservoir., 'a vented cap disposed over the second end of the outflow lumen and comprising2. The biological fluid sampling device of claim 1 , further comprising a luer lock at least partially surrounding the access lumen.3. The biological fluid sampling device of claim 1 , wherein the vented cap comprises a luer lock for insertion in the outflow lumen.4. The biological fluid sampling device of claim 3 , wherein the vented cap comprises a fastener for removably connecting a ...

RAPID CONCENTRATION, RECOVERY AND DETECTION OF PATHOGENS IN FOOD SAMPLES

Methods for rapidly concentrating a food sample for efficient detection of bacteria are disclosed. A microfiltration approach followed by centrifugation was used to concentrate the cells with an enzyme (e.g., a protease) added at the beginning of the process to facilitate more efficient micro-filtering. The enzyme was found to have no significant effect on cell viability. 1. A method for detecting pathogens in food samples , the method comprising:obtaining a food sample;treating the food sample with an enzyme;microfiltering the treated food sample; andassaying the microfiltered food sample for presence of a pathogen,wherein cellular viability of the pathogen is maintained throughout the treating and microfiltering steps.2. The method of wherein the treated food sample comprises a solution.3. The method of further comprising preparing the food sample before the treating step wherein preparing comprises mechanically blending the food sample.4. The method of wherein the prepared food sample comprises coagulated proteins.5. The method of wherein the food sample comprises egg.6. The method of wherein the food sample comprises chicken.7. The method of claim 1 , wherein the food sample comprises spinach.8. The method of claim 1 , wherein the food sample comprises beef.9. The method of claim 1 , wherein the food sample comprises turkey.10. The method of claim 1 , wherein treating the food sample comprises hydrolyzing proteins in the food sample.11. The method of claim 10 , wherein the enzyme comprises is a protease.12. The method of wherein the treating step comprises incubating the food sample with the protease for less than about 90 minutes.13. The method of wherein the enzyme comprises a lipase.14. The method of wherein the assaying step comprises plating the microfiltered food sample on a selective media to detect the pathogen.15. The method of wherein the assaying step comprises a polymerase chain reaction (PCR)-based detection of nucleic acid of the pathogen. ...

UNDERWATER GAS MEASUREMENT APPARATUS FOR GASES DISSOLVED IN WATER

An underwater gas measuring device for gases dissolved in water. The device can be used at great water depths, in particular in the deep-sea water column or on the sea floor. The underwater gas measuring device is better suited to perform a very large number of measurements than the pre-known measuring devices. The underwater gas measuring device preferably has at least one pressure tank downstream of the gas outlet with a gas outlet into the environment, the gas outlet being opened as soon as the internal pressure of the pressure tank exceeds the ambient pressure. 1. An underwater gas measuring device for gases dissolved in water comprising:{'b': 170', '170, 'a. a pressure vessel () with a gas inlet opening in the wall of the pressure vessel ();'}{'b': '30', 'b. a semi-permeable, gas-permeable membrane () arranged at the gas inlet opening in a watertight manner;'}{'b': 40', '30, 'c. a support device () for supporting the membrane () against hydrostatic pressure;'}{'b': 60', '30, 'd. a measuring chamber () in the pressure vessel in gas exchanging communication with the membrane () and having a gas outlet;'}{'b': 75', '60, 'e. a detection device () for detecting at least one physical and/or chemical parameter of gas in the measuring chamber ();'}{'b': 160', '75, 'f. an electronic evaluation device () designed to detect at least one signal of the detection device () representing at least one detected parameter and designed for digitized transmission and/or for non-volatile digital storage of at least one signal and/or at least one derivative signal;'}{'b': 90', '60, 'g. a vacuum pump () connected downstream of the gas outlet of the measuring chamber ();'}{'b': 100', '90, 'h. a non-return valve () connected downstream of the vacuum pump () in the flow direction;'}{'b': 130', '100, 'i. a collecting chamber () downstream of the non-return valve () for measured gas;'}{'b': 165', '90', '30', '130, 'j. a pump control device () which is designed to cause the vacuum pump () ...

Method and associated device for rapid detection of target biomolecules with enhanced sensitivity

A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.

Assays to detect neurodegeneration

Methods of measuring the amount of singly- or multiply-phosphorylated p217+ tau protein in a sample are provided. Methods of detecting or diagnosing tauopathies, methods of determining the effectiveness of a treatment of a tauopathy, and methods of determining whether a subject is suitable for anti-p217+ tau antibody therapy are also provided. Also described are antibodies for use in the methods and kits comprising the antibodies.

DETECTION METHOD FOR HEAVY METAL IONS

Provided is a detection method for heavy metal ions that includes the following steps. A waste water is flowed through an ion-imprinted polymer tube for adsorbing at least two kinds of target heavy metal ions. The ion-imprinted polymer tube is rinsed to remove a non-target object from the ion-imprinted polymer tube. The target heavy metal ions in the ion-imprinted polymer tube are desorbed by using an acid liquid. An electrochemical method is performed to detect concentrations of the target heavy metal ions. 1. A detection method for heavy metal ions , comprising:flowing a waste water through an ion-imprinted polymer tube for adsorbing at least two kinds of target heavy metal ions;rinsing the ion-imprinted polymer tube to remove a non-target object from the ion-imprinted polymer tube;desorbing the target heavy metal ions in the ion-imprinted polymer tube by using an acid liquid; andperforming an electrochemical method to detect concentrations of the target heavy metal ions.2. The detection method for the heavy metal ions of claim 1 , wherein the at least two kinds of target heavy metal ions in the waste water are simultaneously adsorbed by the ion-imprinted polymer tube.3. The detection method for the heavy metal ions of claim 1 , wherein the ion-imprinted polymer tube has at least two kinds of ion-printed polymers.4. The detection method for the heavy metal ions of claim 1 , wherein a conductivity of the waste water is greater than or equal to 2 claim 1 ,000 microSiemens/cm.5. The detection method for the heavy metal ions of claim 1 , wherein the ion-imprinted polymer tube is rinsed with a deionized water.6. The detection method for the heavy metal ions of claim 1 , wherein the target heavy metal ions comprise at least two kinds of lead ions claim 1 , copper ions claim 1 , chromium ions claim 1 , nickel ions claim 1 , zinc ions claim 1 , and cadmium ions.7. The detection method for the heavy metal ions of claim 1 , wherein the acid liquid comprises a sulfuric acid ...

MODIFIED FRANZ CELL

The present invention relates to an acceptor chamber for a Franz diffusion cell, comprising an upper opening closable by a membrane and, on the side wall of the acceptor chamber, an upper and a lower outlet opening designed to remove or add liquid medium from the acceptor chamber, the upper outlet opening being located within the upper two thirds of the side wall and the lower outlet opening is located in the lower quarter of the side wall, the upper and lower outlet openings being substantially opposite each other, to a Franz diffusion cell comprising a donor compartment and such an acceptor chamber, the donor compartment and the acceptor chamber being separated from each other by a membrane, and to a method for determining the diffusion of a substance through a membrane with such a Franz diffusion cell. 1. An acceptor chamber for a Franz diffusion cell , comprising an upper opening closable by a membrane and , on the side wall of the acceptor chamber , an upper and a lower outlet opening designed to remove or add liquid medium from the acceptor chamber , wherein the upper outlet opening is placed within the upper two thirds of the side wall and the lower outlet opening is placed in the lower quarter of the side wall , characterised in that the upper and lower outlet openings are located substantially opposite each other.2. The acceptor chamber according to claim 1 , characterised in that the upper and/or the lower outlet opening is reclosable.3. The acceptor chamber according to claim 1 , characterised in that the acceptor chamber has the basic shape of a cylinder claim 1 , cuboid or cube.4. The acceptor chamber according to claim 1 , characterised in that the lower outlet opening is arranged flush with the bottom of the acceptor chamber.5. The acceptor chamber according to claim 1 , characterised in that the upper outlet opening is placed within the upper third of the side wall.6. The acceptor chamber according to claim 1 , characterized in that the acceptor ...

APPARATUS, SYSTEMS, AND METHODS FOR ISOTOPE EXCHANGE AND/OR DIALYSIS

A dialysis and/or isotope exchange device includes a support, a sample chamber having a thickness of between 25 μm and 500 μm, and a container including a semipermeable membrane in contact with the sample chamber. Dialysis and/or isotope exchange takes less than 100,000 seconds for a concentration of a substance to reach a target concentration. 1. A dialysis device , comprising:a support;a container including a semipermeable membrane on one end of the container; anda sample chamber having a thickness of between 25 μm and 500 μm.2. The device of claim 1 , wherein the sample chamber is recessed within the support.3. The device of claim 1 , wherein the sample chamber is at least partially defined by a volume in a spacer located between the support and the container.4. The device of claim 3 , wherein the spacer includes double-sided adhesive tape.5. The device of claim 1 , wherein a sample cross-sectional area of the sample chamber is less than a membrane cross-sectional area of the semipermeable membrane.6. The device of claim 1 , wherein the support is a glass support.7. The device of claim 1 , wherein the sample chamber has a volume of between 1 μl and 50 μl.8. The device of claim 1 , wherein the semipermeable membrane has a molecular cut-off weight of between approximately 1 claim 1 ,000 Dalton and 300 claim 1 ,000 Dalton.9. An isotope exchange device claim 1 , comprising:a sample having a first concentration of a substance in a sample chamber, the sample chamber located on a support, wherein the sample chamber has a sample chamber thickness of between 25 μm and 500 μm;a dialysate having a second concentration of the substance in a dialysate container; anda semipermeable membrane on one end of the dialysate container, the semipermeable membrane having a molecular cut-off weight greater than a molecular weight of the substance, wherein the semipermeable membrane is in contact with the sample.10. The device of claim 9 , wherein the substance includes DO.11. The device ...

ASPIRATION-FREE WELL PLATE APPARATUS AND METHODS

A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir. 120-. (canceled)21. An apparatus , comprising:a well plate including a top portion, a bottom portion, and a membrane disposed between the top portion and the bottom portion,the top portion including a sample well defining an interior volume in fluid communication with an opening defined by the membrane and with a reservoir defined by the bottom portion,the membrane including a first portion in fluid communication with the interior volume of the sample well, a second portion in fluid communication with a first portion of the well plate disposed outside the interior volume of the sample well, and a third portion in fluid communication with a third portion of the well plate disposed outside the interior volume of the sample well, the third portion of the well plate being different than the second portion of the well plate,the well plate configured to be used in a centrifugation process of a test sample received within the sample well and the reservoir, the test sample including a sample material and a wash liquid; andthe membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the ...

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a multi-component blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to separate the plasma portion from the cellular portion. After separation, the biological fluid collection device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid collection device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid collection device is engageable with a testing device for closed transfer of a portion of the plasma portion from the biological fluid collection device to the testing device. The testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid collection device for a multi-component blood sample , comprising:a housing having an inlet port, an outlet port, a first flow channel defined within the housing and in fluid communication with the inlet port, and a second flow channel defined within the housing and in fluid communication with the outlet port; anda valve disposed between the first flow channel and the second flow channel and transitionable between a closed position and an open position, wherein with the valve in the closed position, the first flow channel is in fluid isolation from the second flow channel, and wherein with the valve in the open position, the first flow channel is in fluid communication with the second flow channel,wherein the second flow channel comprises a collection chamber having a separation member disposed therein and a blood component chamber defined therein in communication with the separation member.2. The biological fluid collection device of claim 1 , wherein the inlet port is adapted to receive the multi- ...

Biological Fluid Sampling Transfer Device and Biological Fluid Separation and Testing System

A biological fluid sampling transfer device that is adapted to receive and separate a multi-component blood sample is disclosed. After separation, the biological fluid sampling transfer device is able to transfer a plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device of the present disclosure also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with an anticoagulant. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results. 1. A biological fluid sampling transfer device adapted to receive a multi-component blood sample , the biological fluid sampling transfer device comprising:a housing having an inlet port and an outlet port, the inlet port and the outlet port in fluid communication;a filter disposed between the inlet port and the outlet port, the filter adapted to restrain at least a first part of the multi-component blood sample, and to allow at least a second part of the multi-component blood sample to pass therethrough;a compression element disposed between the inlet port and the outlet port, the compression element spaced from the filter; anda holding element movable within the housing between a first position, in which the holding element receives the second part of the multi-component blood sample from the filter, and a second position, in which the compression element is adapted to direct the second part of the multi-component blood sample from the holding element to the outlet port.2. The biological fluid sampling transfer device of claim 1 , wherein the first part of the multi-component blood sample is a cellular component and the ...

Liquid to Liquid Biological Particle Concentrator

A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. 1flowing a foam wash from a permeate side of the hollow fiber filter across the hollow fiber filter to the feed side of the hollow fiber filter such that the amount of biological particulates become concentrated in the foam wash;wherein a pressurized wash liquid and water-soluble gas mixture is released from under pressure and agitated to create the foam wash.. A method for extracting an amount of biological particulates from a feed side of a hollow fiber filter, the method comprising: This application is a continuation of U.S. patent application Ser. No. 13/368,197, filed Feb. 7, 2012, now U.S. Pat. No. 8,758,623; which is a continuation of U.S. patent application Ser. No. 12/131,015, filed May 30, 2008, now U.S. Pat. No. 8,110,112; which claims priority to U.S. Provisional Patent Application Ser. No. 60/932,271, filed May 30, 2007, and to U.S. Provisional Patent Application Ser. No. 60/961,391, filed Jul. 20, 2007; the contents of which are hereby incorporated by reference herein in their ...

Whole Blood Analytic Device And Method Therefor

Devices and methods are presented in which a plasma separation device with a first and second portion separates a blood containing fluid. Most preferably, the first portion produces a cell fraction and a plasma fraction, and the second portion captures the plasma fraction. A first actuator then fluidly isolates a portion of the plasma fraction within the second portion, and a second actuator moves the isolated portion of the plasma fraction from the second portion. 1. An analytic device , comprising:a sample receiving compartment;a plasma separation device that is at least partially disposed in the sample receiving compartment and comprises (1) a first portion configured to separate blood into a cell fraction and a plasma fraction, and (2) a second portion configured to capture at least a portion of the plasma fraction;wherein the analytic device comprises a flexible first area that, when compressed by a first actuator, exerts pressure on a first part of the second portion and thereby restricts flow of the plasma fraction between the first and the second portion;wherein the analytic device has a third flexible area that is fluidly coupled to the sample receiving compartment and that, when compressed by a third actuator, allows for flow of a reagent from the third flexible area to a second part of the second portion to thereby mix the reagent with the plasma fraction in the second part of the second portion; andwherein the analytic device has a flexible second area that, when compressed by a second actuator, allows for flow of the mixture out of the sample receiving compartment.2. The device of claim 1 , wherein the first portion comprises a hydrophobic separation membrane.3. The device of claim 1 , wherein the second portion comprises a hydrophilic membrane having a void volume for collection of a predetermined volume of plasma.4. The device of claim 1 , wherein the first and second portions are fluidly coupled to each other to allow capillary flow of the plasma ...

Stirred Cell And Method of Using Same

Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.

METHOD AND APPARATUS FOR THE FILTRATION OF BIOLOGICAL SOLUTIONS

A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration. 1. A filtration system comprising:a plurality of sections, each section having at least one channel providing at least one serial flow path, each section being in fluid communication with each adjacent section preceding it and being in fluid communication with each adjacent stage that follows it;each of the at least one channel comprising a filtration membrane and having a length, a membrane area, a void volume, a specific membrane area expressed as a ratio of the membrane area to the void volume, and a dimensionless length expressed as a product of the channel length and the specific membrane area; wherein a dimensionless length of a stage is the sum of the dimensionless lengths of each channel in the longest serial flow path in the stage and the dimensionless length of the system is the sum of the dimensionless lengths of the plurality of sections;wherein the dimensionless length of the system is greater than about 2,000 and the dimensionless length of at least one of the plurality of sections is less than about 6,000; and a spiral-wound cartridge; and', 'a spiral-wound module., 'wherein at least one of the plurality of sections comprises one of2. The filtration system of claim 1 , wherein each of the plurality of sections is substantially identical.3. The filtration system of claim 2 , wherein each of the substantially identical sections includes a series-parallel arrangement of channels.4. The filtration system of claim 1 , wherein the specific membrane area of at least one channel is greater than about 40 cm.5. A filtration system comprising:a plurality of sections, each stage having at least one channel ...

MEMBRANE VESICLE RECOVERY DEVICE, MEMBRANE VESICLE RECOVERY METHOD, AND MEMBRANE VESICLE ANALYSIS METHOD

A membrane vesicle recovery device includes: a liquid filler; and at least a fused membrane having a lipid bilayer membrane which covers at least a part of the outer periphery of the liquid filler, in which a content of a membrane vesicle is mixed into the liquid filler through fusing of the membrane vesicle and the fused membrane. 1. A membrane vesicle recovery device , comprising:a liquid filler; andat least a fused membrane including a lipid bilayer membrane which covers at least a part of an outer periphery of the liquid filler, whereina content of a membrane vesicle is mixed into the liquid filler through fusing of the membrane vesicle and the fused membrane.2. The membrane vesicle recovery device according to claim 1 , further comprising:a reaction base having a surface on which a plurality of holding sections which is configured to hold the liquid filler are formed, whereinthe liquid filler is covered with the fused membrane in each of the holding sections.3. The membrane vesicle recovery device according to claim 2 , whereinthe holding sections are recessed sections formed in the reaction base,in the recessed sections, the fused membrane comes into contact with an opening end forming a boundary between the surface and each of the recessed sections and is provided in the reaction base so as to block the recessed sections, andthe recessed sections are filled with the liquid filler.4. The membrane vesicle recovery device according to claim 3 , whereina plurality of the fused membranes are provided in the reaction base so as to come into contact with a part along the opening end in the inner wall surface of the recessed sections and to individually block each of the plurality of recessed sections.5. The membrane vesicle recovery device according to claim 4 , whereinin the reaction base, at least the inner wall surface is hydrophobic and a hydrophobic section of the fused membrane comes into contact with the inner wall surface.6. The membrane vesicle recovery ...

Liquid to Liquid Biological Particle Concentrator

A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. 1. A method for extracting an amount of biological particulates from an interior feed side of a hollow fiber filter having a bore , the method comprising:flowing a foam wash from one end to another end of the bore of the hollow fiber filter such that the amount of biological particulates becomes concentrated in the foam wash, resulting in a desired amount of a concentration of biological particulates; andcollecting the desired amount for analysis of the biological particulates.2. The method in claim 1 , comprising flowing a process feed material that bears particulates entrained in a fluid through the hollow fiber filter to separate the amount of biological particulates from the fluid by trapping the amount of biological particulates on the interior feed side of the hollow fiber filter.3. The method in claim 2 , further comprising pulling a vacuum on an exterior permeate side of the hollow fiber filter to pull the process feed material through the hollow fiber filter.4. The method in claim 1 , ...

SALIVA COLLECTION, PROCESSING, STABILIZATION, AND STORAGE METHOD

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and/or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness. 1. A method for stabilizing nucleic acid and protein samples isolated from a saliva sample , the method comprising:a) collecting a saliva sample from a subject;b) filtering the saliva sample to produce a filtered sample that is free of cells;c) collecting the filtered sample in at least a first and a second receiving device;d) adding an alcohol solution to the first receiving device to produce an alcohol-containing filtered sample comprising a protein sample, with the proviso that alcohol is not added to the second receiving device to produce an alcohol-free filtered sample comprising a nucleic acid sample; wherein the protein sample and the nucleic acid sample are stabilized for at least 3 days when stored at 25 degrees Celsius; ande) performing an analysis on the filtered sample collected in the first and second receiving devices comprising one or more of: a protein analysis on the alcohol-containing filtered sample or a nucleic acid analysis on the alcohol-free filtered sample.2. The method of claim 1 , wherein the nucleic acid is DNA.3. The method of claim 1 , wherein the nucleic acid analysis is polymerase chain reaction (PCR).4. The method of claim 1 , wherein the nucleic acid is RNA.5. The method of claim 4 , wherein the nucleic acid analysis is RT-PCR.6. The ...

SOLID PHASE EXTRACTION CARTRIDGE

Provided is a solid phase extraction cartridge including a front end portion for discharging in a cartridge main body, which is capable of connecting a pipe without using a connection adapter and is preferable for an extremely small amount filling type with high analysis accuracy. 1: A solid phase extraction cartridge which is configured by , in a cylindrical cartridge main body , attaching a sample outflow-side frit , filling a portion above the sample outflow-side frit with a solid phase filler , and causing a sample inflow-side frit to abut against and be pressed on the solid phase filler to be capped , and is provided with a cylindrical front end portion for discharging under the sample outflow-side fit in the cartridge main body ,wherein an inner surface of the front end portion for discharging is formed into a tapered shape of being decreased in diameter toward a sample inflow side, and a pipe inserted into the front end portion for discharging from a sample outflow side makes close contact with the inner surface at the sample inflow side.2: A solid phase extraction cartridge which is configured by , in a cylindrical cartridge main body , attaching an integrated solid phase extraction body having a multiple continuous hole or being porous , and is provided with a cylindrical front end portion for discharging at a sample outflow side of the solid phase extraction body in the cartridge main body ,wherein an inner surface of the front end portion for discharging is formed into a tapered shape of being decreased in diameter toward a sample inflow side, and a pipe inserted into the front end portion for discharging from the sample outflow side makes close contact with the inner surface at the sample inflow side.3: The solid phase extraction cartridge according to claim 1 ,wherein an inner surface of the cartridge main body at the sample inflow side relative to the sample inflow-side fit or an inner surface of the cartridge main body at the sample inflow side ...

Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device

A sampling method using a sampling device () comprising a container () comprising a body () consisting of a soft pouch, and a lid (), in which at least one among the container () and the lid () is provided with a discharging member () suitable for, in an open state, discharging at least one part of the gases contained in the internal space () of the body () of the container (), wherein the sampling method comprises the steps consisting in: collecting a biological material in the body () of the container (), and assembling the lid () on the neck (IO) of the container (), and placing an internal space () of the body () of the container () under anaerobic conditions by placing the discharging member () in the open state, by compressing the body () of the container () and by placing the discharging member () in a closed state. 122. A sampling device () for sampling microorganisms contained in biological matter , the sampling device () comprising:{'b': 5', '6', '7', '10', '12', '7', '6', '6', '5, 'a container () comprising a body () which comprises an internal space () configured to receive a biological matter, and a neck () which delimits an access opening () to the internal space () of the body (), the body () of the container () being constituted by a flexible bag,'}{'b': 30', '10', '5', '12', '10', '7', '6, 'a cover () configured to be removably and sealingly mounted on the neck () of the container () so as to obturate the access opening () of the neck () and close the internal space () of the body (),'}{'b': 5', '30', '25', '40', '45', '48', '7', '25', '40', '45', '48', '25', '40', '45', '48', '7', '25', '40', '45', '48', '7, 'wherein at least one of the container () and the cover () is provided with at least one transfer member (, , , ) internally comprising a passage between the internal space () and an external environment, the transfer member (, , , ) having an open state in which said transfer member (, , , ) establishes communication with the internal space () ...

ANALYTE EXTRACTION

A target analyte is extracted out of a sample fluid in a sample fluid passage by diffusing the target analyte through a supported liquid membrane to a product fluid passage. Extraction of the target analyte is accelerated by applying an electric field across and perpendicular to the supported liquid membrane with electrodes. Passage of selected ions across an exchange membrane extending between one of the electrodes and the supported liquid membrane is inhibited. 1. An analyte extraction system comprising:a first electrode;a second electrode to cooperate with the first electrode to form an electric field therebetween;a supported liquid membrane between the first electrode and the second electrode;an exchange membrane between the first electrode and supported liquid membrane;a first buffer fluid passage between the first electrode and the first exchange membrane;a sample input passage between the first exchange membrane and the supported liquid membrane; anda product output passage between the second exchange membrane and the supported liquid membrane.2. The analyte extraction system of further comprising an analyte sensor within the product output passage.3. The analyte extraction system of claim 2 , wherein the analyte sensor comprises a surface enhanced Ramen spectroscopy sensor.4. The analyte extraction system of further comprising:a second exchange membrane between a first electrode and the supported liquid membrane; anda second fluid passage between the second electrode and the second exchange membrane.5. The analyte extraction system of further comprising:a second supported liquid membrane between the supported liquid membrane in the second exchange membrane; anda waste fluid passage between the second supported liquid membrane and the second electrode.6. The analyte extraction system of further comprising:a first pump to move a sample fluid through the sample input passage;a second pump to move a product fluid through the product output passage; anda ...

MICRO-SCALE LIQUID-LIQUID-LIQUID EXTRACTION

The present disclosure relates to systems and methods for extraction of analytes (e.g from bodily fluids). In some embodiments, the systems and methods utilize plates (e.g., 96 well) plates and a supported liquid membrane to extract analytes of interest from biological other samples. 1. A system , comprising:a) a donor plate comprising a multi well plate comprising a plurality of samples comprising an analyte of interest; andb) an acceptor plate comprising a solid support coated with a liquid membrane.2. A system , comprising:a) an acceptor plate comprising a multi well plate comprising a plurality of acceptor solutions; andb) a donor plate comprising a multi well plate comprising a plurality of solid supports coated with liquid membranes, and a plurality of samples comprising an analyte of interest.3. The system of claim 1 , wherein said multiwall plate is a 96-well plate.4. The system of claim 1 , wherein said solid support is an inert porous polymer support.5. The system of claim 1 , wherein said porous solid polymer support is made from polypropylene claim 1 , polyethylene claim 1 , polysulfone claim 1 , polytetrafluoroethylene claim 1 , or polyvinylidene difluoride.6. The system of claim 1 , wherein said porous solid polymer support comprises pores that are 0.50 μm or smaller.7. The system of claim 1 , wherein said porous solid polymer support comprises pores that are 0.45 μm or smaller.8. The system of claim 1 , wherein said liquid membrane is an organic solvent.9. The system of claim 8 , wherein said organic solvent is selected from the group consisting of dihexyl ether claim 8 , dodecyl acetate claim 8 , n-hexadecane claim 8 , isopentyl benzene claim 8 , hexyl decanol claim 8 , and kerosene.10. A method claim 8 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'a) contacting a system of with a plurality of samples comprising an analyte of interest; and'}b) transferring said analyte of interest from said acceptor plate to said donor plate.11. The ...

METHODS AND DEVICES FOR THE SEPARATION, DETECTION AND MEASUREMENT OF MOLECULES IN LIQUID SAMPLES

The invention relates to the field of microfluidic detection and analyses. Described herein are methods and devices for the separation, detection and measurement of molecules in liquid samples. Certain aspects of the invention concerns the separation of analyte(s) of interest based on the combined use of: (i) membrane separation, (ii) an electric field and/or a magnetic field; and (iii) capillary action and/or gravity. Envisioned applications include separation, detection and/or measurement of analytes in blood samples, food samples and environmental samples. One particular example is a portable biosensor for the detection and measurement of histamine and diamine oxidase (DOA) in a drop of blood. 133.-. (canceled)34. A device for the detection of at least one analyte in a liquid sample , comprising:a separation zone for depositing a liquid sample comprising at least one analyte to be detected and other constituents, wherein said at least one analyte has a greater size and a charge different from other constituents in the liquid sample;a reaction zone for receiving and reacting said at least one analyte;a neutral zone in between the separation zone and the reaction zone, the neutral zone allowing flow said at least one analyte from the separation zone to the reaction zone;wherein said at least one analyte is detected at the reaction zone by at least one of amperometry, potentiometry, colorimetry and photometry.35. The device of claim 34 , wherein said membrane comprises pores having a size allowing passage of said at least one analyte but not passage of constituents in the liquid sample.36. The device of claim 34 , wherein said separation zone comprises: (i) a first electrode extending above said membrane and positioned to be in fluid communication with an upper surface of the microporous membrane; and (ii) a second electrode extending below said membrane and positioned to be in fluid communication with a lower surface of the microporous membrane; each of said ...

SIMULTANEOUS ISOLATION AND PRECONCENTRATION OF EXOSOMES BY ION CONCENTRATION POLARIZATION METHOD AND APPARATUS

Exosomes carry microRNA biomarkers, occur in higher abundance in cancerous patients than in healthy ones, and because they are present in most biofluids, including blood and urine, can be obtained non-invasively. Standard laboratory techniques to isolate exosomes are expensive, time-consuming, provide poor purity, and recover on the order of 25% of the available exosomes. We present a new microfluidic technique to simultaneously isolate exosomes and preconcentrate them by electrophoresis using a high transverse local electric field generated by ion-depleting ion-selective membrane. We use pressure-driven flow to deliver an exosome sample to a microfluidic chip such that the transverse electric field forces them out of the cross flow and into an agarose gel which filters out unwanted cellular debris while the ion-selective membrane concentrates the exosomes through an enrichment effect. We efficiently isolated exosomes from 1×PBS buffer, cell culture media and blood serum. Using flow rates from 150 μL/hr to 200 μL/hr and field strengths of 100 V/cm, we consistently captured between 60% to 80% of exosomes from buffer, cell culture media, and blood serum as confirmed by both fluorescence spectroscopy and nanoparticle tracking analysis. Our microfluidic chip maintained this recovery rate for more than twenty minutes with a concentration factor of 15 for ten minutes of isolation. 1. A method of simultaneous isolation and preconcentration of exosomes by ion concentration polarization comprisingproviding a sample medium including exosomes with microRNA biomarkers;separating a first portion of the sample from a second portion of the sample with an ion-depleting ion-selective membrane wherein the membrane generating a high transverse local electric field;delivering pressure-driven flow to move the medium bearing the exosome sample to the membrane;separating the exosome sample from the medium in the transverse electric field into a filter gel to remove debris; ...

INTEGRATED MEMBRANE DEVICE

An apparatus is disclosed for separating and preserving biomolecules of a biological fluid sample. The apparatus includes an assembly having sides forming a hollow shape having a first opening at one end and second opening at the opposite end, a sample mixing chamber positioned adjacent the first opening within the assembly, the sample mixing chamber from which a flow of the biological fluid sample is actuated in a direction from the sample mixing chamber to the first matrix layer, the sample mixing chamber being in a direction downstream of the first opening, a first valve positioned between the sample mixing chamber and the first matrix layer, the first valve configured to control the flow to the first matrix layer, a first input in fluid communication with the sample mixing chamber and positioned upstream of the first valve, a second input positioned between the first matrix layer and the second matrix layer, and a second valve positioned between the second matrix layer and the second opening, the second valve configured to control the flow to the second matrix layer. 1. An apparatus for separating and preserving biomolecules of a biological fluid sample having , the apparatus comprising: sides forming a hollow shape with an opening at each of two ends forming a first opening and a second opening;', 'a sample mixing chamber positioned adjacent the first opening within the assembly, the sample mixing chamber from which a flow of the biological fluid sample is actuated in a direction from the sample mixing chamber to the second opening, the sample mixing chamber being downstream of the first opening;', 'two matrix layers positioned within the hollow shape between the sample mixing chamber and the second opening, the two matrix layers comprising a first matrix layer and a second matrix layer;', 'a first valve positioned between the sample mixing chamber and the first matrix layer, the first valve configured to control the flow out of the sample mixing chamber and to ...

Biological Fluid Transfer Device and Biological Fluid Sampling System

A biological fluid sampling transfer device adapted to receive a multi-component blood sample is disclosed. After collecting the blood sample, the biological fluid sampling transfer device is able to separate a plasma portion from a cellular portion. After separation, the biological fluid sampling transfer device is able to transfer the plasma portion of the blood sample to a point-of-care testing device. The biological fluid sampling transfer device also provides a closed sampling and transfer system that reduces the exposure of a blood sample and provides fast mixing of a blood sample with a sample stabilizer. The biological fluid sampling transfer device is engageable with a blood testing device for closed transfer of a portion of the plasma portion from the biological fluid sampling transfer device to the blood testing device. The blood testing device is adapted to receive the plasma portion to analyze the blood sample and obtain test results.

AUTOMATION OF BARRIER-BASED PLANT NUCLEIC ACID AND PROTEIN EXTRACTION

This invention is related to systems and methods for nucleic acid/protein extraction/isolation from biological samples. In particular, automated barrier-based systems for samples from plant tissues/cells are disclosed. The automated systems and methods disclosed provide reliability and high throughput specifically suitable for nucleic acid/protein extraction/isolation from plant tissues/cells. In some embodiments, the automated systems disclosed can further enhance high throughput capacity for nucleic acid/protein extraction/isolation directly from plant tissue samples. 1. An automated system for nucleic acid extraction/isolation from a sample of plant tissue or plant cells , comprising ,(a) at least one barrier-based sample processing apparatus;(b) a mobility mechanism for transporting the sample processing apparatus;(c) a plurality of magnetic beads and at least one magnetic field generator;(d) at least one liquid reagent; and(e) a mechanism for adding and aspirating the liquid reagent.2. The automated system of claim 1 , further comprising a centrifuge device.3. The automated system of claim 1 , wherein the automated system does not comprise a shaker for mixing solution with the sample processing apparatus.4. The automated system of claim 1 , wherein the at least one barrier-based sample processing apparatus comprises:(i) an input zone for receiving the sample therein;(ii) a first reaction zone for receiving a first reagent therein; and(iii) a second reaction zone downstream of the first reaction zone for receiving a second reagent therein; and(iv) a force movable between a first position adjacent the input zone and a second position adjacent the second reaction zone; wherein: the force urges the fraction-bound solid phase substrate from the input zone and into the first reaction zone; and the force urges a first portion of the fraction-bound solid phase substrate into the second reaction zone.5. The automated system of claim 1 , wherein the at least one barrier- ...

Biological Fluid Collection Device and Biological Fluid Separation and Testing System

A biological fluid collection device that is adapted to receive a blood sample having a cellular portion and a plasma portion is disclosed. After collecting the blood sample, the biological fluid collection device is able to transfer the blood sample to a point-of-care testing device or a biological fluid separation and testing device. After transferring the blood sample, the biological fluid separation and testing device is able to separate the plasma portion from the cellular portion and analyze the blood sample and obtain test results. 1. A biological fluid collection device , comprising:a lancet housing having an inlet port and an interior defining a first flow channel in fluid communication with the inlet port, a second flow channel in fluid communication with the first flow channel, and a chamber spaced apart from the inlet, wherein at least a portion of the second flow channel is diverted from the first flow channel;a puncturing element moveable between a pre-actuated position wherein the puncturing element is retained within the interior of the housing and a puncturing position wherein the puncturing element extends through the inlet port of the housing and provides fluid communication with the first flow channel; anda transfer cartridge having a reservoir,wherein the first flow channel is in fluid communication with the chamber, and the second flow channel is in fluid communication with the reservoir of the transfer cartridge.2. The biological fluid collection device of claim 1 , wherein the chamber is dimensioned to receive the first blood provided to the first flow channel through the inlet port.3. The biological fluid collection device of claim 1 , wherein the chamber is vented.4. The biological fluid collection device of claim 1 , wherein the chamber contains a wicking material.5. The biological fluid collection device of claim 1 , further comprising a passive valve between the first flow channel and the chamber.6. The biological fluid collection device ...

AMPHOTERIC DISSOCIATION ION EXCHANGE MEDIUM AND USES THEREOF AND METHOD FOR CALIBRATING SEPARATION CAPACITY THEREOF

An amphoteric dissociation ion exchange separation medium, the surface of which is an amphoteric dissociation covalently-modified layer. When an environmental pH value is lower than the isoelectric point, pIm, of the covalently-modified layer, the type of net charges on the surface of the covalently-modified layer is positive and the separation medium has the properties of an anion exchanger; when the environmental pH value is higher than the pIm, the type of net charges on the covalently-modified layer surface is negative and the separation medium has the properties of a cation exchanger. The separation medium has the properties of an anion exchanger and a cation exchanger at both sides of the pIm, respectively. The pH of an eluent can be adjusted to allow the separation medium surface and the target substance to have the same type of net charges, so that the target substance can be released by electrostatic repulsion. 1. An amphoteric dissociation ion exchange separation medium , wherein a surface of the amphoteric dissociation ion exchange separation medium is an amphoteric dissociation covalently-modified layer; the amphoteric dissociation covalently-modified layer has an isoelectric point (pIm) that is an environmental pH value at which a net charge on the surface of the amphoteric dissociation ion exchange separation medium is zero; wherein when the environmental pH value is lower than the pIm , the net charge on a surface of the amphoteric dissociation covalently-modified layer is positive and the amphoteric dissociation ion exchange separation medium acts as an anion exchanger; when the environmental pH value is higher than the pIm , the net charge on the surface of the amphoteric dissociation covalently-modified layer is negative and the amphoteric dissociation ion exchange separation medium acts as a cation exchanger;the amphoteric dissociation covalently-modified layer on the surface of the amphoteric dissociation ion exchange separation medium comprises ...

Device for Cross Flow Filtration

A whole blood filtration device is provided with a filter membrane separating a feeding volume and a clean side of the filter membrane from each other. The feeding volume communicates with a first feeding side opening and with a second feeding side opening. The filter membrane has pores with a pore size that ensures permeability of the filter membrane to blood plasma/serum and that retains blood cells. The first feeding side opening can be coupled to a first blood pump for feeding blood from the first feeding side opening into the feeding volume so that blood plasma/serum permeates the filter membrane and blood cells, retained by the filter membrane, exit from the feeding volume through the second feeding side opening.

RARE CELL ISOLATION DEVICE, RARE CELL ISOLATION METHOD, AND RARE CELL DETECTION METHOD USING THE SAME

The present invention provides a rare cell isolation device including: a first body which is disposed above a filtration membrane and includes a first inlet for injecting a biospecimen; and a second body which is disposed under the first body and bonded to the filtration membrane, wherein the first body and the second body have a disk-shaped structure to be rotatable around their centers, and the filtration membrane is disposed to be separated from the center of the second body in a radial direction. 1. A rare cell isolation device comprising:a filtration membrane which filters a biospecimen;a first body which is disposed above the filtration membrane and includes a first inlet for injecting the biospecimen; anda second body which is disposed under the first body and bonded to the filtration membrane,wherein the first body and the second body have a disk-shaped structure to be rotatable around their centers, andthe filtration membrane is disposed to be separated from the center of the second body in a radial direction.2. The rare cell isolation device of claim 1 , further comprisinga filtrate storage unit which is formed at a contact portion between the first body and the second body and is connected to the filtration membrane.3. The rare cell isolation device of claim 2 , whereinthe first body includes:an upper plate through which the first inlet penetrates; anda first intermediate plate which is coupled to a lower part of the upper plate, on its one side.4. The rare cell isolation device of claim 3 , whereinthe first body includesa first guiding unit which is formed at a contact portion between the upper plate and the first intermediate plate and of which one side is connected to the first inlet and the other side is connected to the filtration membrane.5. The rare cell isolation device of claim 4 , whereinthe upper plate includes a ventilation hole which is connected to the first guiding unit.6. The rare cell isolation device of claim 4 , whereinthe second body ...

PARTICULATE MATTER SENSOR

Microfabricated PM sensors measure concentrations of particulate matter (PM) in air. Some sensors improve the accuracy of measurements by accounting for the effect of ambient conditions (e.g., temperature or humidity) on mass-sensitive elements employed to determine a mass of the PM in a stream of air. Some sensors improve the accuracy of measurements by controlling humidity in the stream of air measured by mass-sensitive elements. Some sensors employ a plurality of mass-sensitive elements to extend the useful life of the PM sensor. Some sensors employ one or more mass-sensitive elements and heating elements to cause deposition and allow measurement of different sizes of PM. Some sensors can measure mass concentration of coarse PM in addition to fine PM in a stream of air. Some sensors control the flow rate of a stream of air measured by mass-sensitive elements. Some sensors include features to mitigate electromagnetic interference or electromagnetic signal loss. 1. A sensor for detecting particulate matter (PM) pollutants , comprising:a body defining a main channel configured to receive a stream of air from outside the body via one or more fractionators configured to select for a particular size of PM; and a first of the mass-sensitive elements is disposed in the main channel and configured to receive a deposition of PM from the stream of air and to provide a first signal indicating a mass of the PM deposition; and', 'a second of the mass-sensitive elements configured not to receive the PM deposition and configured to provide a second signal indicating an effect of one or more ambient conditions on the mass-sensitive elements., 'a plurality of mass-sensitive elements, wherein2. The sensor of claim 1 , wherein the effect of the one or more ambient conditions on the first mass-sensitive element includes at least one of a temperature effect or a humidity effect.3. The sensor of claim 1 , wherein the plurality of mass-sensitive elements are resonators claim 1 , and ...

Devices, systems, and methods for removal of soluble gases from fluid samples

Devices, systems and methods are disclosed which relate to using containers with a multitude of nucleation sites covering a major portion of the inside wall of the container to enable rapid and nearly complete removal of soluble gases from fluid samples, including carbonated beverages and other carbonated fluid samples. A fluid sample is rapidly poured into the described container initiating a catastrophic release of the soluble gas from the sample.

METHOD AND KIT FOR SAMPLE PREPARATION AND ENDOTOXIN DETERMINATION

The invention relates to a method for preparation of a sample () of a formulation () for subsequent endotoxin determination, the formulation () suspected of comprising an endotoxin, the formulation () preferentially being a pharmaceutical formulation. The method comprises the following steps: application of the sample () to an endotoxin-free centrifugation column () containing a size exclusion chromatography matrix () that has been equilibrated with a suitable equilibration buffer () and elution of a flow through () of the sample by centrifugation, which flow through () can then be used for endotoxin determination. The equilibration buffer () is selected according to a subsequently used method of endotoxin determination, the equilibration buffer () only containing components not interfering with subsequently used method of endotoxin determination. Furthermore, the invention relates to a kit () for preparation of a sample (). 110111111. Method for preparation of a sample () of a formulation () for subsequent endotoxin determination , the formulation () suspected of comprising an endotoxin , the formulation () preferentially being a pharmaceutical formulation , the method comprising the following steps:21025666. application of the sample () to an endotoxin-free centrifugation column () containing a size exclusion chromatography matrix () that has been equilibrated with a suitable equilibration buffer (); wherein the equilibration buffer () is selected according to a subsequently used method of endotoxin determination , the equilibration buffer () only containing components not interfering with subsequently used method of endotoxin determination;31515. elution of a flow through () of the sample by centrifugation , which flow through () can then be used for endotoxin determination.46102615. Method according to claim 1 , wherein excess equilibration buffer () is removed prior to application of the sample () onto the centrifugation column () by a centrifugation step claim ...

METHOD AND APPARATUS FOR ANALYSIS OF IONSIZED GASES

Apparatus comprising an inlet region for receiving a gas mixture; an ionizer for supplying hydronium ions to the received gas mixture to generate ions, wherein the ionizer comprises a membrane for receiving the gas mixture, and wherein the membrane, preferably made of graphene oxide, is capable of generating hydronium ions from water; and an ion detector for detecting ions generated from the gas mixture. 115-. (canceled)16. An apparatus , comprising: an ionizer for supplying hydronium ions to the gas mixture to generate ions, wherein the ionizer comprises a membrane for receiving the gas mixture, and wherein the membrane is capable of generating hydronium ions from water; and', 'an ion detector for detecting ions generated from the gas mixture., 'an inlet region for receiving a gas mixture;'}17. The apparatus according to claim 16 , wherein the membrane is a graphene oxide membrane or a membrane of a material capable of generating hydronium ions when in contact with water.18. The apparatus according to claim 16 , further comprising a drift region for separating ions according to their ion mobility.19. The apparatus according to claim 18 , wherein the drift region comprises at least one of: a drift tube and a drift medium.20. The apparatus according to claim 18 , wherein the drift region comprises a graphene oxide membrane.21. The apparatus according to claim 16 , wherein the membrane is selectively permeable.22. The apparatus according to claim 16 , wherein the ionizer comprises a stack of graphene oxide membranes for receiving the gas mixture and for separating ions according to their mobility.23. The apparatus according to claim 16 , wherein the apparatus is a portable apparatus for detecting volatile organic compounds in a gas and/or vapour phase sample claim 16 , such as exhaled breath or an environmental sample.24. A method claim 16 , comprising:contacting water molecules with a membrane capable of generating hydronium ions from water;contacting a gas mixture ...

METHOD FOR RECOVERING MICROBIAL CELLS

The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same. 2. The method of claim 1 , wherein the sample is contacted with the buffer solution and protease(s) separately.3. The method of claim 1 , wherein the buffer solution and protease(s) are mixed prior to step (b).4. The method of any one of to wherein the sample is or comprises a clinical sample.5. The method of any one of to claim 1 , wherein the sample is a clinical claim 1 , biological or environmental sample added to a medium.6. The method of wherein the medium is a culture medium.7. The method of any one of to wherein the clinical sample is a blood sample.8. The method of wherein said blood sample is collected in a blood culture flask.9. The method of any one of to wherein said protease is proteinase K.10. The method of any one of to wherein the pH of the buffer solution is between pH 7 and less than pH 10.7.11. The method of any one of to wherein the pH of the buffer solution is between pH 7 and pH 10.5.12. The method of any one of to wherein the pH of the buffer solution is between pH 9 and pH 10.5.13. The method of any one of to claim 7 , wherein the filter is a membrane filter.14. The method of any one of to claim 7 , wherein the filter is a polyamide filter.15. The method of any one of to claim ...

SAMPLE PREPARATION DEVICES AND METHODS

A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter. 1. A device for sample processing , the device comprising:at least one chamber having an egress, said chamber being configured to receive a sample for processing;a filter through which at least some sample portions in the at least one chamber flow;a barrier member disposed in a first state to contain sample in the at least one chamber; andat least a second chamber comprising a collection chamber fluidically connected in series with the at least one chamber and separated from flow communication with the at least one chamber via the barrier member in the first state,wherein, upon sufficient conditions, the barrier member is alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter,wherein the filter is configured to permit passage of target molecules contained in a sample introduced into the at least one chamber.2. The device of claim 1 , wherein the filter is configured to permit passage of sample portions smaller than a threshold size and block passage of sample portions of at least the threshold size.3. (canceled)4. The device of claim 1 , wherein the filter is configured to block passage of at least some sample portions that are insoluble in a lysis medium.5. The device of claim 1 , wherein the filter comprises at least one of a frit and a functionalized resin.6. The device of claim 1 , wherein claim 1 , at least in the first ...

ADDING ION SENSITIVITY TO AN INTEGRATED COMPUTATIONAL ELEMENT (ICE)

A device including an ion-selective membrane arranged within an optical path of the device and coupled to a sample cell to interact with a fluid sample and thereby modify an optical response of the ion-selective membrane according to an ion con-centration in the fluid sample, is provided. The device also includes an integrated computational element (ICE) arranged within the optical path, so that the illumination light optically interacts with the ICE and with the ion-selective membrane to provide a modified light that has a property indicative of the ion concentration in the fluid sample. A detector that receives the modified light provides an electrical signal proportional to the property of the modified light. A method and a system for using the above device are also provided. 1. A device , comprising:an ion-selective membrane arranged within an optical path of the device and coupled to a sample cell to interact with a fluid sample and thereby modify an optical response of the ion-selective membrane according to an ion concentration in the fluid sample, wherein the optical path of the device is defined by an illumination light;an integrated computational element (ICE) arranged within the optical path, wherein the illumination light optically interacts with the ICE and with the ion-selective membrane to provide a modified light that has a property indicative of the ion concentration in the fluid sample; anda detector that receives the modified light and provides an electrical signal proportional to the property of the modified light.2. The device of wherein the sample cell facilitates optical interaction between the illumination light and the ion-selective membrane to generate a sample light claim 1 , and the sample light interacts with the ICE to provide the modified light.3. The device of claim 1 , wherein the ion-selective membrane absorbs a pre-selected type of ions from the fluid sample up to an equilibrium value determined by the ion concentration in the ...

POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES

A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications. 1. A filtration device comprising:an inlet layer including a first volume and a first inlet layer opening to said first volume;a filter structure including a single polymer filter layer with uniform thickness formed from negative photo-definable dry film having a first filter surface and a second filter surface diametrically opposite to said first filter surface, and a plurality of apertures each extending through the polymer filter layer, said filter structure having flexibility allowing for disposing on a roll and unrolling; andan outlet layer including a second volume and a first outlet layer opening to said second volume,wherein said first filter surface is exposed to said first volume,said second filter surface is exposed to said second volume,at least a filtered portion of a sample entering said first inlet layer opening, passes from said first volume to said second volume via said filter layer and exits said second volume via said first outlet layer opening,said filter structure retaining on said first filter surface at ...

DEVICE FOR EXTRACTING VOLATILE SPECIES FROM A LIQUID

The invention relates to a device () for extracting volatile species from a liquid () connected to an inlet of an analysis instrument, such as a mass spectrometer (MS). The device has a chamber (), a membrane () forming a barrier for the liquid at zero differential pressure between the inside and the outside of the chamber, and allowing passage of the volatile species at zero differential pressure between the inside and the outside of the chamber. The device has an inlet capillary channel () to feed in a carrier gas and prevent back-diffusion from the chamber, and an outlet capillary channel () which provides a significant pressure reduction, e.g. from atmospheric pressure in the chamber () to near-vacuum suitable for an MS. The invention combines the best of two worlds, i.e. the fast time-response of a DEMS system and the high sensitivity of a MIMS system, since a differential pumping stage is not needed. 1. A device for extracting volatile species from an associated liquid and being suitable for connection to an inlet of an associated analysis instrument , such as a mass spectrometer , the device comprising:a. a chamber, wherein the volume of the chamber is 1 ml or less, i. connects the inside of the chamber with the outside of the chamber,', 'ii. forms a barrier for the liquid at zero, or near-zero, differential pressure between the inside and the outside of the chamber,', 'iii. allows passage of the volatile species at zero, or near-zero, differential pressure between the inside and the outside of the chamber,, 'b. a membrane, whichc. an inlet capillary channel which connects the inside of the chamber with the outside of the chamber, the inlet capillary channel being arranged for intake of a carrier gas, andd. an outlet capillary channel which connects the inside of the chamber with the outside of the chamber, the outlet capillary channel being arranged for connection with said inlet of the associated analysis instrument, wherein the outlet capillary channel is ...

ULTRA-SENSITIVE MULTI-TARGET LATERAL FLOW MOLECULAR ASSAY WITH FIELD-INDUCED PRECIPITATION

Described are methods for detecting and quantifying biomolecules such as polynucleotides or polypeptides in an electrophoresis matrix using ion concentration polarization and nanoparticle aggregation. 1. A method for separating biomolecules , the method comprising: a microchannel having a first end and a second end and containing an electrophoresis matrix,', 'an ion permselective membrane in direct contact with the electrophoresis matrix, and', 'a first electrode and a second electrode configured to apply an electric field across the ion permselective membrane;, 'a) providing a microfluidic device comprising'}b) loading a sample containing a plurality of biomolecules, a plurality of first probes and a plurality of second probes into the electrophoresis matrix, wherein each of the first probes comprises a first nanoparticle coupled to a plurality of first binding moieties, and each of the second probes comprises a second nanoparticle coupled to a plurality of second binding moieties, and wherein the first binding moieties and second binding moieties are configured to bind target biomolecules within the plurality of biomolecules;c) applying a first electric field that causes the plurality of biomolecules, the plurality of first probes and the plurality of second probes to move through the electrophoresis matrix towards the ion permselective membrane whereupon the plurality of biomolecules, the plurality of first probes and the plurality of second probes become concentrated in the electrophoresis matrix adjacent to the ion permselective membrane, wherein at least some of the target biomolecules bind to one of the plurality of first probes and one of the plurality of second probes to form a plurality of linked nanoparticle multimers comprising at least one first nanoparticle and at least one second nanoparticle; andd) applying a second electric field to form an ion depletion front as a result of ion concentration polarization, whereupon the ion depletion front moves ...

Forward Osmosis Coupled Electrochemical Sensor

Devices and systems and methods for concentrating and electrochemically detecting an analyte in an aqueous sample are provided. These devices, systems, and methods are useful for detecting an analyte, which as naturally found or produced, occurs at a concentration below that which can be measured using conventional methods.

SYSTEMS AND METHODS FOR QUANTIFYING AND MODIFYING PROTEIN VISCOSITY

Systems and methods for determining regions of proteins that contribute to the viscosity of formulations of those proteins are provided. Methods for modifying the viscosity of concentrated protein formulations are also provided. 1. A method for identifying regions in a protein that contribute to the viscosity of the protein , comprising:microdialysing samples of the protein in a microdialysis cartridge against a buffer comprising deuterium for at least two different time periods;subsequently quenching the microdialysis of the samples;analyzing the quenched samples in an hydrogen/deuterium exchange mass spectrometry system to determine regions of the protein in the sample that have reduced levels of deuterium relative to other regions of the protein, wherein regions of the protein that have reduced levels of deuterium contribute to the viscosity of the protein.2. The method of claim 1 , wherein samples of protein comprise between 10 mg/mL to 200 mg/mL of protein.3. The method of claim 1 , wherein samples of protein in the microdialysing step are in a buffer having a pH between 5.0 and 7.5.4. The method of claim 1 , wherein the samples of protein in the microdialysing step are in 10 mM Histidine at pH 6.0.5. The method of claim 1 , wherein the buffer comprising deuterium comprises 10 mM Histidine at pH 6.0.6. The method of claim 1 , wherein the microdialysis is performed at 2 to 6° C.7. The method of claim 1 , wherein at least one sample is microdialysed for 4 hours and at least another sample is microdialysed for 24 hours.8. The method of claim 1 , wherein the quenching step is performed at −2 to 2° C. for 1 to 5 minutes.9. The method of claim 1 , further comprising digesting the protein into peptides before mass spectrometry analysis.10. A method of modifying the viscosity of a protein drug claim 1 , comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'identifying regions of the protein drug that contribute to the viscosity of the protein drug according to ...

Method and device of using aqueous two-phase systems (ATPS) for enhancing diagnostics for sexually transmitted infections

This invention relates to a method and device for improving the accuracy and performance of detecting or diagnosing sexually transmitted infections (STIs) or STI-causing pathogens. In one embodiment, the present method and device are related to removing one or more interfering molecules such as urea from urine sample, where these interfering molecules alter the performance of Lateral-Flow Immunoassay (LFA). In one embodiment, an aqueous two-phase system (ATPS) embedded entirely within a porous material allows spontaneous phase separation and the target STI-causing pathogens is concentrated in one of the separated phases. In one embodiment, a detection module such as the Lateral-Flow Immunoassay (LFA) is used in connection with other modules so as to detect or diagnose the sexually transmitted infections or the pathogens associated with STIs with an improved performance. 2. The system of claim 1 , wherein said purifying unit comprises a purifying agent that forms a precipitate by reacting with said interfering molecules claim 1 , said precipitate is retained in the purifying unit and does not migrate with liquid flow claim 1 , or said precipitate does not interfere with the detection of said pathogen.3. The system of claim 1 , wherein said purifying agent is selected from the group consisting of citric acid claim 1 , lactic acid claim 1 , acetic acid claim 1 , and activated carbon.4Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalisTreponema pallidum. The system of claim 1 , wherein said pathogen is selected from the group consisting of and (syphilis) claim 1 , and Human Immunodeficiency Virus.5. The system of claim 1 , wherein the porous material is selected from the group consisting of fiber-glass paper claim 1 , cotton-based paper claim 1 , single-layer matrix paper claim 1 , and polyolefin foam pad.6. The system of claim 1 , wherein said ATPS components are selected from the group consisting of polymers claim 1 , salts claim 1 , and surfactants.7. ...

Liquid to Liquid Biological Particle Concentrator

A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. 1. A method for extracting an amount of biological particulates from an interior feed side of a hollow fiber filter having a bore , the method comprising:flowing a foam wash from one end to another end of the bore of the hollow fiber filter such that the amount of biological particulates becomes concentrated in the foam wash, resulting in a desired amount of a concentration of biological particulates; andcollecting the desired amount for analysis of the biological particulates.2. The method in claim 1 , comprising flowing a process feed material that bears particulates entrained in a fluid through the hollow fiber filter to separate the amount of biological particulates from the fluid by trapping the amount of biological particulates on the interior feed side of the hollow fiber filter.3. The method in claim 2 , further comprising pulling a vacuum on an exterior permeate side of the hollow fiber filter to pull the process feed material through the hollow fiber filter.4. The method in claim 1 , ...