18-04-2023 дата публикации
Номер: CN115976152A
Принадлежит:
The invention relates to the technical field of tissue digestion and separation, in particular to a use method of a centrifugal device for tissue digestion, which comprises the following steps: transferring a mixture of digestive juice and a to-be-digested tissue block to the bottom of a centrifugal tube through an operation window; placing the centrifugal tube in an environment with a proper temperature for incubation, blowing and beating the mixture up and down by using a pipetting device every 10-15 minutes, then blowing and beating the mixture to a cell filter screen by using the pipetting device, collecting a cell suspension in the centrifugal tube, and repeatedly blowing and beating and cutting tissue blocks by using the filter screen until most tissue blocks are digested; and filtering through a cell filter screen until all the residual tissue blocks are enriched on the cell filter screen, so as to obtain a cell suspension without the residual tissue blocks in the centrifugal tube ...
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