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PLASMID STANDARD FOR USE IN QUANTITATIVE ASSAYS USING FLUORESCENT QUANTITATIVE PCR

Номер патента: US20130217115A1. Автор: Chen Zhao, LI JUN, MO Minli, Xu Junpu. Владелец: . Дата публикации: 22-08-2013.
Disclosed is a plasmid standard for use in fluorescent quantitative PCR assays. More specifically, the present invention provides a plasmid standard as well as amplification primers and detection probes thereof for use in the detection of gene mutation and expression amount. 1. A plasmid standard for detection by fluorescent quantitative PCR , characterized in that the vector for said plasmid is pMD18-T , and the insert for said plasmid is any one of following (1)-(4) sequence to be detected , which is for detecting the mutation of corresponding gene ,(1) EGFR Exon 18, 19, 21;(2) KRAS Codon 12, 13;(3) BCRP position 482 amino acid; or(4) BRAF position 600 amino acid.2. A plasmid standard according to claim 1 , characterized in that the insert for said plasmid is:(1) SEQ ID NO: 209, SEQ ID NO: 210, SEQ ID NO: 211, SEQ ID NO: 212, SEQ ID NO: 213, SEQ ID NO: 214, SEQ ID NO: 215, SEQ ID NO: 216;(2) SEQ ID NO: 217, SEQ ID NO: 218, SEQ ID NO: 219, SEQ ID NO: 220, SEQ ID NO: 221, SEQ ID NO: 222;(3) SEQ ID NO: 223, SEQ ID NO: 224, SEQ ID NO: 225; or(4) SEQ ID NO: 226, SEQ ID NO: 227.3. A plasmid standard according to claim 1 , characterized in that the primer for preparing and detecting said plasmid is:(1) SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23;(2) SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38;(3) SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 49; or(4) SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56. ...

Device and method for controlling power according to a load of a hybrid excavator

Номер патента: US20130265686A1. Автор: Gyeong-Mo MIN. Владелец: VOLVO CONSTRUCTION EQUIPMENT AB. Дата публикации: 10-10-2013.
A power control apparatus and method according to a load of a hybrid excavator including an H-ECU is disclosed. If a difference between an actual power value actually used in a load (SW PEC and ISAM PEC) or in an ESS and a power value required for an actual use in the load is equal to or larger than a set error value, a power that is supplied from the ESS to the PECs and a power that is supplied between the PECs are controlled to be cut off, and thus high voltage is prevented from being continuously supplied to circuits to thereby prevent secondary problems (e.g., fire, trouble of equipment, and the like) from occurring due to the high voltage.

COIL ASSEMBLY AND MOTOR INCLUDING THE SAME

Номер патента: US20220069658A1. Автор: Kim Yong Ho, KOO Min Mo. Владелец: . Дата публикации: 03-03-2022.
The present disclosure relates to a coil assembly including: a plurality of unit flat coils disposed and spaced apart from one another in a circumferential direction; and a molding body made by dual injection molding and configured to partially surround the unit flat coils, thereby simplifying a structure of the coil assembly and a process of manufacturing the coil assembly. 1. A coil assembly comprising:a plurality of unit flat coils disposed and spaced apart from one another in a circumferential direction; anda molding body including a structure formed in dual injection molding and configured to partially surround the plurality of unit flat coils.2. The coil assembly of claim 1 , wherein each of the plurality of unit flat coils comprises:a first flat coil having a first cross-sectional shape; anda second flat coil having a second cross-sectional shape different from the first cross-sectional shape and insulated from the first flat coil by the molding body.3. The coil assembly of claim 2 , wherein the molding body comprises:a plurality of unit molding portions disposed on the respective unit flat coils and configured to partially surround the unit flat coils; anda connection portion configured to connect adjacent unit molding portions of the plurality of unit molding portions to each other.4. The coil assembly of claim 3 , wherein each of the plurality of unit molding portions comprises:a first accommodation portion configured to accommodate the first flat coil; anda second accommodation portion spaced apart from the first accommodation portion and configured to accommodate the second flat coil.5. The coil assembly of claim 3 , wherein the connection portion connects first ends of the adjacent unit molding portions to each other claim 3 , and second ends of the adjacent unit molding portions are disposed to be spaced apart from each other.6. The coil assembly of claim 1 , wherein each of the plurality of unit flat coils is configured by a conductor that is free of ...

MACHINING, EXAMINATION, AND WELDING INTEGRAL DEVICE FOR NOZZLE

Номер патента: US20140150228A1. Автор: Cho Hong Seok, Cho Ki Huyn, CHOI SANG HOON, Jang Kyong Soon, LEE Jang Wook, Mo Min Hwan. Владелец: Korea Plant Service & Engineering Co., Ltd.. Дата публикации: 05-06-2014.
The present invention relates to a machining, examination, and welding integral device for a nozzle, and more particularly, to a machining, examination, and welding integral device for a nozzle in which machining, examination, and welding can be made in the nozzle by one device. To this end, there is provided a machining, examination, and welding integral device for a nozzle including: a pair of caps provided to be separated from each other; a welding unit provided between the pair of caps to weld a junction part of the nozzle and a pipe; a examination unit coupled to one side of the welding unit to check whether the junction part is defective; and a machining unit coupled to the top of the welding unit to remove the surface of a welded portion of the junction part and/or process the welded portion of the junction part. 1. A machining , examination , and welding integral device for a nozzle , comprising:a pair of caps provided to be separated from each other;a welding unit provided between the pair of caps to weld a junction part of the nozzle and a pipe;a examination unit coupled to one side of the welding unit to examine whether the junction part is defective; anda machining unit coupled to the top of the welding unit to remove the surface of a welded portion of the junction part and/or process the welded portion of the junction part.2. The machining claim 1 , examination claim 1 , and welding integral device for a nozzle of claim 1 , wherein:foreign substance preventing layers are provided on surfaces of the pair of caps facing each other.3. The machining claim 2 , examination claim 2 , and welding integral device for a nozzle of claim 2 , wherein:a support member is provided between the welding unit and the foreign substance preventing layer.4. The machining claim 1 , examination claim 1 , and welding integral device for a nozzle of claim 1 , further comprising:a control unit controlling the welding unit, the machining unit, and the examination unit.5. The ...

Kit For Detecting PIK3CA Gene Mutation

Номер патента: US20180112271A1. Автор: Chen Zhao, DING Feng, Li Hui, LI JUN, MO Minli. Владелец: . Дата публикации: 26-04-2018.
The present invention relates to a kit for detecting PIK3CA gene mutation, and this kit can be used to detect cancer-related PIK3CA gene mutation. The said kit comprises: (1) the internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dNTP solution; (2) the PIK3CA mutation detection reagent, which includes the PIK3CA gene mutant type specific primers, PIK3CA gene mutant type specific probes, internal control gene specific primers, internal control gene specific probes and dNTP solution; (3) the Taq DNA polymerase; and (4) the PIK3CA positive quality control. 1. A kit for detecting PIK3CA gene mutation , comprising:(1) an internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dNTP solution,in which the said internal reference gene specific primers are SEQ ID No: 1 and SEQ ID No: 2;the said internal reference gene specific probe is SEQ ID No: 14;(2) an PIK3CA mutation detection reagent, which includes the PIK3CA gene mutant type specific primers, PIK3CA gene mutant type specific probes, internal control gene specific primers, internal control gene specific probes and dNTP solution,in which the said PIK3CA gene mutant type is selected from:PM1, i.e. PIK3CA gene Exon 9 1624G>A;PM2, i.e. PIK3CA gene Exon 9 1633G>A;PM3, i.e. PIK3CA gene Exon 9 1635G>T;PM4, i.e. PIK3CA gene Exon 20 3140A>G; andPM5, i.e. PIK3CA gene Exon 20 3140A>T;for PM1 mutation, the said PIK3CA gene mutant type specific primers are SEQ ID NO: 5 and SEQ ID NO: 8;for PM2 mutation, the said PIK3CA gene mutant type specific primers are SEQ ID NO: 6 and SEQ ID NO: 8;for PM3 mutation, the said PIK3CA gene mutant type specific primers are SEQ ID NO: 7 and SEQ ID NO: 8;for PM4 mutation, the said PIK3CA gene mutant type specific primers are SEQ ID NO: 9 and SEQ ID NO: 11;for PM5 mutation, the said PIK3CA gene mutant type specific primers ...

STATOR ASSEMBLY OF HAIRPIN WINDING MOTOR AND MANUFACTURING METHOD THEREOF

Номер патента: US20210175784A1. Автор: Kim Joon Hyung, Kim Yong Ho, KOO Min Mo. Владелец: . Дата публикации: 10-06-2021.
A stator assembly of a hairpin winding motor including hairpin coils continuously connected along a circumferential direction to form a coil winding; and a stator core configured to fix the hairpin coils. Outer circumferential surfaces of the hairpin coils formed in the circumferential direction contact an inner circumferential surface of the stator core. 1. A stator assembly of a hairpin winding motor comprising:a plurality of hairpin coils continuously connected along a circumferential direction to form a coil winding; anda stator core configured to fix the hairpin coils,wherein outer circumferential surfaces of the hairpin coils formed in the circumferential direction contact an inner circumferential surface of the stator core.2. The stator assembly of claim 1 , wherein:each of the hairpin coils includes a head portion exposed to the outside of the stator core in an axial direction, and leg portions extending from one end and the other end of the head portion and configured to be inserted into a core slot of the stator core; andthe leg portions have a shape which is repeated in the circumferential direction by one pole pitch to be continuously connected.3. The stator assembly of claim 2 , wherein a cross-section of the leg portion has a trapezoidal shape of which the outside is wider than the inside claim 2 , such that a width thereof decreases in a direction toward an inner diameter from an outer diameter of the stator core.4. The stator assembly of claim 3 , wherein the stator core includes a ring core formed in a cylindrical shape and in which the hairpin coils are disposed claim 3 , and a plurality of pin cores are inserted into the ring core to fix the hairpin coils to the ring core.5. The stator assembly of claim 4 , wherein the ring core includes:a yoke formed of a magnetic material to form a magnetic path;a plurality of assembly parts protruding from an inner circumferential surface of the yoke in a radial direction; andcore slots formed between the ...

SYSTEM FOR RAPIDLY DETECTING INFECTIOUS AGENTS USING A HYBRIDOMA-BASED BIOSENSOR

Номер патента: US20140273020A1. Автор: Kittle J.D., Mo Min, VEDAMOORTHY Srikanth, ZENG Lingchun, ZUPANCIC Thomas J.. Владелец: APPLIED BIOMOLECULAR TECHNOLOGIES. Дата публикации: 18-09-2014.
A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and at least one biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; wherein the biosensor emits a detectable signal when it reacts with the specific infectious agent; wherein the biosensor is a hybridoma cell that naturally expresses an endogenous anti-target antigen specific IgM; and wherein the hybridoma cell has been converted to a B cell receptor biosensor by introducing a detectable reporter gene into the cell. 1) A biosensor , comprising: a hybridoma cell that naturally expresses an endogenous anti-target antigen specific IgM; wherein the hybridoma cell has been converted to a B cell receptor biosensor by introducing a detectable reporter gene into the cell; wherein the biosensor is operative to detect a specific infectious agent in a sample to be tested; and wherein the biosensor emits a detectable signal when it reacts with the specific infectious agent.2) The biosensor of claim 1 , wherein the detectable reporter gene is an aequorin reporter gene.3Escherichia coli.) The biosensor of claim 1 , wherein the specific infectious agent is4) The biosensor of claim 1 , wherein the sample to be tested is derived from beef claim 1 , poultry claim 1 , fish claim 1 , or vegetable matter.5) The biosensor of claim 1 , wherein the detectable signal further includes light.6) A system for rapidly detecting infectious agents in biological samples claim 1 , comprising:(a) at least one sample to be tested for at least one specific infectious agent; and(b) at least one biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; wherein the biosensor emits a detectable signal when it reacts with the specific infectious agent; wherein the biosensor is a hybridoma cell that naturally expresses an endogenous anti- ...

STRIPPING TIP FOR ORTHODONTICS AND MANUFACTURING METHOD THEREOF

Номер патента: US20170196656A1. Автор: MO Hyun-Min, SHIN Sang-Nam. Владелец: . Дата публикации: 13-07-2017.
The present invention relates to a stripping tip for orthodontics and a manufacturing method thereof. The stripping tip includes a grinding blade provided with a grinding surface for grinding teeth, a holder comprising a grinding blade coupling portion coupled to the grinding blade and a connection coupling portion connected to the grinding blade coupling portion and coupled to a handle or handpiece, and a stiffness enhancement portion provided to a part of the grinding blade coupling portion coupled to the grinding blade to enhance stiffness of the part of the grinding blade coupling portion coupled to the grinding blade. 1. A stripping tip for orthodontics comprising:a grinding blade provided with a grinding surface for grinding teeth;a holder comprising a grinding blade coupling portion coupled to the grinding blade and a connection coupling portion connected to the grinding blade coupling portion and coupled to a handle or handpiece; anda stiffness enhancement portion provided to a part of the grinding blade coupling portion coupled to the grinding blade to enhance stiffness of the part of the grinding blade coupling portion coupled to the grinding blade.2. The stripping tip according to claim 1 , wherein the stiffness enhancement portion is formed by performing pressure forging on a surface of the grinding blade coupling portion coupled to the grinding blade.3. The stripping tip according to claim 1 , wherein the stiffness enhancement portion is formed by compressing a part of the grinding blade coupling portion in a thickness direction such that a groove is provided to a surface of the grinding blade coupling portion coupled to the grinding blade claim 1 ,wherein a step is formed between a surface of a part of the grinding blade coupling portion provided with the stiffness enhancement portion and a surface of a remaining part of the grinding blade coupling portion,wherein movement of the grinding blade coupled to the stiffness enhancement portion in a ...

Nras gene mutation detection kit

Номер патента: US20170198355A1. Автор: FENG DING, HUI Li, Jun Li, Minli Mo, Zhao Chen. Владелец: Jiaxing Accb Diagnostics Ltd. Дата публикации: 13-07-2017.
This invention relates to a kit for detecting NRAS gene mutation, and this kit can be used to detect cancer-related NRAS gene mutation. The said kit comprises: (1) the internal reference detection reagent, which includes the internal reference gene specific primers, internal reference gene specific probes and dNTP solution; (2) the NRAS mutation detection reagent, which includes the NRAS gene mutant type specific primers, NRAS gene mutant type specific probes, internal control gene specific primers, internal control gene specific probes and dNTP solution; (3) the Taq DNA polymerase; and (4) the NRAS positive quality control.

METHOD FOR SIGNAL AMPLIFICATION IN BIOSENSOR-BASED SYSTEM FOR RAPIDLY DETECTING INFECTIOUS AGENTS

Номер патента: US20140335603A1. Автор: Kittle J.D., Mo Min, VEDAMOORTHY Srikanth, ZENG Lingchun, ZUPANCIC Thomas J.. Владелец: APPLIED BIOMOLECULAR TECHNOLOGIES. Дата публикации: 13-11-2014.
A system for detecting infectious agents in biological samples in real time that includes a sample to be tested for at least one specific infectious agent; and a biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits an amplified and detectable signal when it reacts with the specific infectious agent. Signal detection is enhanced through the release of LPS from the cells of the infectious agent. 1. A system for rapidly detecting infectious agents in biological samples , comprising:(a) at least one sample to be tested for at least one specific infectious agent, wherein individual cells of the at least one infectious agent include at least one type of biomolecule that reacts with a biosensor when brought into physical contact therewith, and wherein the at least one type of biomolecule further includes lipopolysaccharides; and(b) at least one biosensor, wherein the biosensor is operative to detect a specific infectious agent in the sample to be tested; and wherein the biosensor emits a detectable signal when it reacts with the lipopolysaccharides.2. The system of claim 1 , further comprising: (i) a reaction chamber for receiving the sample and the biosensor, wherein the reaction chamber has a predetermined internal geometry, and wherein the reaction chamber has been adapted to maximize the detectability of the signal while simultaneously reducing background noise;', '(ii) optionally, at least one stabilizer located in the reaction chamber, wherein the stabilizer is operative to minimize shear force damage to the sample and biosensor during mixing of the sample and the biosensor; and, '(a) a test cartridge for containing the sample and the biosensor, wherein introducing the biosensor into the test cartridge mixes the sample and the biosensor, and wherein the test cartridge further includes(b) a testing unit adapted to receive the test cartridge, wherein the testing unit further ...