Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 13477. Отображено 100.
19-01-2012 дата публикации

Size segregated aerosol mass concentration measurement with inlet conditioners and multiple detectors

Номер: US20120012744A1
Автор: Anthony E. Hase, David A. Lieder, George John Chancellor, James E. Farnsworth, James Evanstad, Jugal K. Agarwal, Sreenath Avula, Xiaoliang Wang
Принадлежит: TSI Inc

A system for measuring size segregated mass concentration of an aerosol. The system includes an electromagnetic radiation source with beam-shaping optics for generation of a beam of electromagnetic radiation, an inlet sample conditioner with adjustable cut-size that selects particles of a specific size range, and an inlet nozzle for passage of an aerosol flow stream. The aerosol flow stream contains particles intersecting the beam of electromagnetic radiation to define an interrogation volume, and scatters the electromagnetic radiation from the interrogation volume. The system also includes a detector for detection of the scattered electromagnetic radiation an integrated signal conditioner coupled to the detector and generating a photometric output, and a processor coupled with the conditioner for conversion of the photometric output and cut-size to a size segregated mass distribution.

Подробнее
Номер записи: 1
02-02-2012 дата публикации

Fluorescence detection device and fluorescence detection method

Номер: US20120025098A1
Автор: Kyouji Doi, Shigeyuki Nakada
Принадлежит: Mitsui Engineering and Shipbuilding Co Ltd

When receiving fluorescence emitted by a measurement object irradiated with laser light emitted from a laser light source unit, a fluorescence detection device generates a modulation signal for modulating the intensity of the laser light and modulates the laser light using the modulation signal. The fluorescence detection device obtains a fluorescent signal of the fluorescence emitted by the measurement object irradiated with the laser light, and calculates, from the fluorescent signal, a fluorescence intensity and the phase delay of the fluorescence with respect to the modulation signal. At the time, the fluorescence detection device controls the operation amounts of the signal level of a DC component of the modulation signal and the gain of amplification just after the output of the fluorescent signal so that the value of a fluorescence intensity signal falls within a preset range. After the operation amounts are settled, the fluorescence detection device calculates the fluorescence intensity and then calculates the fluorescence relaxation time of the fluorescence emitted by the measurement object using the phase delay.

Подробнее
Номер записи: 2
01-03-2012 дата публикации

Method and Device for Microparticle Assay Fluorescence Intensity Reference Intraplex

Номер: US20120053085A1
Автор: Brian P. Hanley
Принадлежит: Individual

A method for making suspended microarray readings from a single sample more reliably accurate. It can be applied to any assay system that uses discrete particles coupled with an assay. Most of these are fluidic systems that read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines a reference set of signal levels on particles with separately identified assays of one sample. By elimination of outliers, averaging, taking ratios of averages, and then taking ratios of assay signal levels against the reference set this method makes possible highly reliable diagnostics. When used standalone, the method uses different signal intensities to better calibrate an instrument. This method compensates for multiple sources of errors that can occur in this type of assay system.

Подробнее
Номер записи: 3
15-03-2012 дата публикации

Single-cell microchamber array

Номер: US20120065082A1
Автор: Albert P. Pisano, Franciscus Albertus Kuypers, Won Chul Lee
Принадлежит: Childrens Hospital Oakland Research Center

An apparatus for analyzing individual cell composition in a heterogeneous cell population may include, in one embodiment, a deposition plate having an array of microwells disposed therein, and a cover plate substantially overlying the deposition plate. A pair of electrodes may be associated with one or more of the microwells, and may be configured to generate an electric field within the associated microwell.

Подробнее
Номер записи: 4
31-05-2012 дата публикации

Particle counting method

Номер: US20120133936A1
Автор: Takehiro Imai, Tomonobu Matsuda, Toshiyuki Abe, Tsutomu Nakajima
Принадлежит: Rion Co Ltd

A particle counting method that can count the number of the particles precisely. The method discriminates a wave pattern of the scattered light from a normal particle (subject of the counting) and a wave pattern of the light scattered by the agitation such as a floating particle, a radiation or changes in the intensity of the light. In one embodiment, a method for counting particles is disclosed which irradiates a light to a sample gas, detects a scattered light from a particle included in the sample gas by a photoelectric conversion device, counts the number of the particles of every particle size division by the output voltage wave pattern of the photoelectric conversion device, calculate a time difference (Ta−T 1 ) from a point (T 1 ) being a peak of output voltage wave pattern and a point (Ta) being a falling detection threshold (A), when the time difference (Ta−T 1 ) is beyond counting cancellation time (B), the output voltage wave pattern is not counted as a particle.

Подробнее
Номер записи: 5
14-06-2012 дата публикации

Blood Measuring Apparatus

Номер: US20120145536A1
Автор: Yoshihiro Niiyama
Принадлежит: Nihon Kohden Corp

A blood measuring apparatus includes: first and second chambers which communicate with each other through an aperture; first and second electrodes which are disposed respectively in the first chamber and the second chamber; and a controller: which performs blood measurement by causing a current to flow between the first and second electrodes in a state where diluted blood is contained in the first chamber and diluting solution is contained in the second chamber; and which performs electrolysis by applying a voltage between the first and second electrodes in a state where diluting solution is contained in the first and second chambers, thereby producing washing solution, and which performs washing on at least the aperture, and the first and second chambers by using the produced washing solution.

Подробнее
Номер записи: 6
08-11-2012 дата публикации

White Blood Cell Analysis System and Method

Номер: US20120282598A1
Автор: Giacomo Vacca, Jiong Wu
Принадлежит: ABBOTT LABORATORIES

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

Подробнее
Номер записи: 7
22-11-2012 дата публикации

Fluorescence detecting method, method for producing fluorescent beads, and fluorescent beads

Номер: US20120295278A1
Автор: Hironori Hayashi, Shigeyuki Nakada
Принадлежит: Mitsui Engineering and Shipbuilding Co Ltd

Two or more kinds of fluorescent beads containing at least two kinds of basic fluorochromes different in fluorescence intensity, fluorescence wavelength, and fluorescence relaxation time from each other, wherein a content ratio between the at least two kinds of basic fluorochromes and absolute amounts of contents of the basic fluorochromes are set so as to be different between different kinds of fluorescent beads. The fluorescent beads are used in a flow cytometer for fluorescence detection. This makes it possible to identify a greater variety of beads than before with high accuracy in a single measurement.

Подробнее
Номер записи: 8
22-11-2012 дата публикации

Sorting of adherent cells by selective transformation of labels

Номер: US20120295798A1
Автор: Frank Hsiung, Paul Patt, Robert M. Archer
Принадлежит: Bio Rad Laboratories Inc

Adherent cells bearing characteristics that are detectable only in the adherent state can be sorted on the basis of these characteristics independently of their adherent state, by applying a transformable label to the entire population of cells, both those bearing the characteristics of interest and those not, in their adherent state and identifying the locations of the cells of interest on the adherent surface. The cells of interest, or all cells other than those of interest, are then selectively treated to transform the labels and achieve differentiation between the cells of interest and the remaining cells. All cells are then released from the adherent state and sorted in the same manner as non-adherent cells but on the basis of whether the labels are transformed or not transformed.

Подробнее
Номер записи: 9
06-12-2012 дата публикации

Particle Analysis in an Acoustic Cytometer

Номер: US20120304749A1
Автор: Gregory Kaduchak, Michael D. Ward
Принадлежит: Los Alamos National Security LLC

The present invention is a method and apparatus for acoustically manipulating one or more particles.

Подробнее
Номер записи: 10
06-12-2012 дата публикации

Multiple Flow Channel Particle Analysis System

Номер: US20120307244A1
Автор: Blair Morad, Donald Francis Perrault, JR., Emanuel Tito Mendes Machado, Johnathan Charles Sharpe, Rudolf Hulspas
Принадлежит: Cytonome ST LLC

A microfluidic multiple channel particle analysis system ( 1 ) which allows particles ( 2 ) from a plurality of particle sources ( 3 ) to be independently simultaneously entrained in a corresponding plurality of fluid streams ( 4 ) for analysis and sorting into particle subpopulations ( 5 ) based upon one or more particle characteristics ( 6 ).

Подробнее
Номер записи: 11
14-03-2013 дата публикации

Fine particle measuring apparatus

Номер: US20130065269A1
Автор: Nao Nitta
Принадлежит: Sony Corp

A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.

Подробнее
Номер записи: 12
02-05-2013 дата публикации

Biochip

Номер: US20130104632A1
Автор: Chin-Kuan Lin, Hsiang-Chia Chen, Pai-Yang Lin, Sheng-Hsiung Weng, Yih-Far Chen
Принадлежит: Wistron Corp

A biochip including a chip body, a first electrode and a second electrode is provided. The body has a first accommodating cavity, a second accommodating cavity and a micro-fluid channel. The micro-fluid channel is connected with the first accommodating cavity and the second accommodating cavity. The first electrode has a first end and a second end. The first end is used for contacting a first probe of a detection apparatus. The second end is positioned in the first accommodating cavity. The second electrode has a third end and a forth end. The third end is used for contacting a second probe of the detection apparatus. The forth end is positioned in the second accommodating cavity.

Подробнее
Номер записи: 13
16-05-2013 дата публикации

Particle Detector and Method for Producing Such A Detector

Номер: US20130120749A1
Автор: Nicoletti Sergio
Принадлежит: Commissariat A L'Energie Atomique Et Aux Energies Alternatives

The invention relates to a particle detector including a substrate () made of a semiconductor material, in which at least one through-cavity () is formed, defined by an input section () and an output section (), wherein the input section thereof is to be connected to an airflow source, said substrate supporting: an optical means including at least one laser source (), and at least one waveguide () connected to said at least one laser source and leading into the vicinity of the output section of said cavity; and photodetector means () located near the output section of said cavity and offset relative to the optical axis of the optical means. 1. A particle detector comprising a substrate in which at least one through-cavity , delimited by an entry cross section and an exit cross section , is formed , its entry cross section being intended to be connected to a source of an air flow , said substrate supporting:optical means comprising at least one laser source and at least one waveguide, which is connected to said at least one laser source and the end of which is located in proximity to the exit cross section of said cavity, andphotodetector means located in proximity to the exit cross section of said cavity and offset with respect to the optical axis of said optical means, in order to detect the scattered light.2. The detector as claimed in claim 1 , wherein the optical means and the photodetector means are located on the same face of the substrate.3. The detector as claimed in claim 1 , wherein said at least one cavity has a variable cross section which decreases from the entry cross section to the exit cross section.4. The detector as claimed in claim 1 , wherein the optical means also comprise at least one photonic device claim 1 , at the opposite end of the waveguide from the laser source.5. The detector as claimed in claim 1 , wherein the photodetector means comprise a plurality of photodetectors associated with a through-cavity.6. The detector as claimed in claim ...

Подробнее
Номер записи: 14
13-06-2013 дата публикации

Methods for sorting particles

Номер: US20130149736A1
Автор: Gary Durack, Gary P. Vandre, Jeffrey D. Wallace, Jeremy T. Hatcher, Lon A. Westfall, Niraj Nayak
Принадлежит: INGURAN LLC

A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

Подробнее
Номер записи: 15
11-07-2013 дата публикации

Measurement of particle morphology using filtration

Номер: US20130174643A1
Автор: David Y.H. Pui, Heinz Fissan, Jing Wang, Sheng-Chieh Chen
Принадлежит: David Y.H. Pui, Heinz Fissan, Jing Wang, Sheng-Chieh Chen

A system and method for characterizing a totality of particles selects a class of the totality of particles having a defined mobility; determines the total particle concentration of the class of particles; filters the class of particles using the filter apparatus and determines a filtered particle concentration indicative of the particles of the class which penetrate the filter apparatus; and determines at least one morphological parameter based on the fraction of particles of a class penetrating the filter apparatus.

Подробнее
Номер записи: 16
18-07-2013 дата публикации

SCATTERED LIGHT MEASURING METHOD

Номер: US20130182252A1
Автор: Haaga Gerhard, Neuendorf Michael, Sieg Raymond, Stengel Karl
Принадлежит:

A scattered light method for measuring particle-dependent parameters of gases, in particular particle-dependent parameters of internal combustion engine exhaust gases or other colloids, includes introducing a particle-containing gas into a measuring chamber; emitting a light beam into the measuring chamber; receiving light scattered by the particles using at least two scattered light sensors, the scattered light sensors generating scattered light sensor signals, each being a function of the light received by the respective scattered light sensor; determining an average particle size from the scattered light sensor signals from at least two scattered light sensors and determining at least one further particle parameter from the previously determined average particle size and the scattered light sensor signals. 110-. (canceled)11. A scattered light method for determining at least one particle-dependent parameter of particle-containing gases , in particular particle-containing exhaust gases of an internal combustion engine , the method comprising:a) introducing a particle-containing gas into a measuring chamber;b) emitting a light beam into the measuring chamber;c) receiving scattered light, which has been scattered on particles contained in the gas, using at least two scattered light sensors, the scattered light sensors generating scattered light sensor signals, each scattered light sensor signal being a function of the scattered light received by the respective scattered light sensor;d) determining an average particle size from the scattered light sensor signals of the at least two scattered light sensors; ande) determining at least one additional particle parameter from the previously determined average particle size and from the scattered light sensor signals.12. The method according to claim 11 , wherein the scattered light sensors are at least one of embodied and situated such that the sensors detect the scattered light claim 11 , which has been scattered at ...

Подробнее
Номер записи: 17
18-07-2013 дата публикации

Cell processing apparatus, sample preparation apparatus, and cell analyzer

Номер: US20130183747A1
Автор: Hironori Kobayashi, Junyi Ding, Koki Tajima, Masakazu Fukuda, Ryuichiro Ebi
Принадлежит: Sysmex Corp

A cell processing apparatus includes a storage container that contains liquid L including a biological sample; a filter that prevents a first cell C 1 in the biological sample from passing and allows a second cell C 2 having a smaller diameter than that of the first cell C 1 to pass; and a filtration cylinder for separating, in the storage container and via the filter, the liquid L into a first liquid L 1 mainly including the first cell C 1 and a second liquid L 2 mainly including the second cell C 2 . A measurement target cell filtered by the filter among other cells can be easily collected.

Подробнее
Номер записи: 18
15-08-2013 дата публикации

Flow Cytometry For High Throughput Screening

Номер: US20130210672A1
Автор: Bruce S. Edwards, Frederick W. Kuckuck, Larry A. Sklar
Принадлежит: STC UNM

The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.

Подробнее
Номер записи: 19
22-08-2013 дата публикации

Spatially Correlated Light Collection from Multiple Sample Streams Excited with a Line Focused Light Source

Номер: US20130214176A1
Автор: Andrew P. Shreve, Gabriel P. Lopez, Pearlson Prashanth Austin Suthanthiraraj, Steven Wayde GRAVES
Принадлежит: STC UNM

An affordable flow cytometry system with a significantly increased analytical rate, volumetric sample delivery and usable particle size including a light beam that interrogates multiple flow streams so as to provide excitation across all of the streams, and an optical objective configured to collect light from the sample streams and image the light onto an array detector.

Подробнее
Номер записи: 20
29-08-2013 дата публикации

Sperm processing methods

Номер: US20130224734A1
Автор: Gary Durack, Gary P. Vandre, Jeffrey D. Wallace, Jeremy T. Hatcher, Lon A. Westfall, Niraj V. Nayak
Принадлежит: INGURAN LLC

A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles.

Подробнее
Номер записи: 21
19-09-2013 дата публикации

METHODS AND SYSTEMS FOR DETECTING OR COLLECTING PARTICLES

Номер: US20130240752A1
Автор: Ma Yuchen
Принадлежит:

Methods and systems for detecting and/or collecting particles are disclosed. At least some of the particles are electrically charged by a charger (). At least some of the charged particles are collected by a collector (). Information indicating the number of the detected/collected particles based on measured electrical charges of the charged particles is obtained by a processor (). 1. An apparatus for detecting or collecting particles , the apparatus comprising:a charger to electrically charge at least some of the particles;a collector to collect at least some of the charged particles; anda processor to obtain information indicative of an amount of the detected/collected particles based on measured electrical charges of the charged particles.219-. (canceled)20. The apparatus of claim 1 , further comprising a diluter to reduce a density of the particles being measured.21. The apparatus of claim 20 , wherein the diluter comprises a plurality of grading chambers.22. The apparatus of claim 21 , further comprising a plurality of connectors between two of the plurality of grading chambers claim 21 , wherein the plurality of connectors each have a different diameter.23. The apparatus of claim 20 , wherein the diluter comprises a sub-chamber and a vacuum valve claim 20 , wherein the one of the plurality of grading chambers is configured to be evacuated through the vacuum valve claim 20 , and wherein the sub-chamber is configured to release its content into the one of the plurality of grading chambers to have a diluted content.24. The apparatus of claim 20 , wherein the diluter comprises a plurality of grading channels claim 20 , each having a different diameter.25. The apparatus of claim 1 , wherein the collector is configured to measure electrical pulses caused by the collected charged particles claim 1 , wherein the measured electrical pulses are above a pulse threshold claim 1 , wherein the collector comprises an electrical pulse detector configured to measure electrical ...

Подробнее
Номер записи: 22
19-09-2013 дата публикации

METHOD AND SYSTEM FOR CALIBRATING A FLOW CYTOMETER

Номер: US20130242296A1
Автор: KENYON Oliver
Принадлежит: OJK CONSULTING LTD

A method and system for calibrating a flow cytometer to a refractive index X, the method comprising: analysing a first mixture of particles in the cytometer and recording the results produced, wherein the first mixture comprises particles having refractive index X, wherein the particles of refractive index X have a plurality of different sizes; analysing a second mixture of particles in the cytometer and recording the results produced, wherein the second mixture comprises particles having refractive index Y, wherein refractive index Y does not equal refractive index X, and wherein the particles of refractive index Y have a plurality of different sizes, wherein there is at least a partial overlap between the particle sizes in the first and second mixtures, and wherein the size of at least one particle of the second mixture is known; and using the results produced by the first and second mixture to calibrate the cytometer to refractive index X. 1. A method of calibrating a flow cytometer to a refractive index X , the method comprising:analyzing a first mixture of particles in the cytometer and recording the results produced, wherein the first mixture comprises particles having refractive index X, wherein the particles of refractive index X have a plurality of different sizes;analyzing a second mixture of particles in the cytometer and recording the results produced, wherein the second mixture comprises particles having refractive index Y, wherein refractive index Y does not equal refractive index X, and wherein the particles of refractive index Y have a plurality of different sizes, wherein there is at least a partial overlap between the particle sizes in the first and second mixtures, and wherein the size of at least one particle of the second mixture is known; andusing the results produced by the first and second mixture to calibrate the cytometer to refractive index X.2. A method of calibrating a flow cytometer as claimed in claim 1 , wherein the second mixture ...

Подробнее
Номер записи: 23
26-09-2013 дата публикации

Sample analyzer and computer program product

Номер: US20130252276A1
Автор: Daigo Fukuma, Noriyuki Narisada, Takaaki Nagai
Принадлежит: Sysmex Corp

A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed.

Подробнее
Номер записи: 24
21-11-2013 дата публикации

INHALER ADAPTOR FOR A LASER DIFFRACTION APPARATUS AND METHOD FOR MEASURING PARTICLE SIZE DISTRIBUTION

Номер: US20130308130A1
Автор: Adamo Benoit, Shah Saiyam, Smutney Chad C.
Принадлежит:

The present disclosure relates to an improved device and methods for adapting to a laser diffraction apparatus used for measuring particle size distribution and density of the plume of a powder composition emitted from a dry powder inhaler. 1. A method of measuring at least one particle characteristic with a laser diffraction apparatus , comprising:providing a device comprising a chamber and configured to hold a breath-powered, dry powder inhaler in a closed environment; said breath-powered inhaler having a body and comprising a dry powder formulation;installing said breath-powered inhaler into said chamber so that the body of said dry powder inhaler is enclosed within said chamber;providing positive pressure into said chamber of said device to create a flow of air or gas through said dry powder inhaler to discharge particles of said dry powder formulation, andmeasuring said at least one particle characteristic with the laser diffraction apparatus,wherein the particles are emitted from the inhaler into a chamberless and/or ambient environment.2. The method of claim 1 , wherein said at least one particle characteristic is particle density.3. The method of claim 1 , wherein said at least one particle characteristic is particle size distribution.4. The method of claim 1 , wherein the breath-powered claim 1 , dry powder inhaler comprises a mouthpiece.5. The method of claim 4 , wherein said mouthpiece forms an air pathway from the chamber to the chamberless and/or ambient environment.6. The method of claim 1 , wherein the step of providing positive pressure into said chamber is attained by a source of pressurized gas from a flow controller system comprising a valve or a syringe pump.7. The method of claim 1 , wherein the positive pressure applied is greater than 1 kPa.8. The method of claim 1 , wherein the step of measuring with a laser diffraction apparatus occurs concurrent with emission of particles from the dry powder inhaler.9. A method of measuring at least one ...

Подробнее
Номер записи: 25
28-11-2013 дата публикации

Device for inspecting a biological fluid

Номер: US20130316440A1
Автор: Nelly Rongeat, Patrick Brunel, Philippe Nerin
Принадлежит: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS, HORIA ABX SA, UNIVERSITE DE LIMOGES

A device for inspecting a biological fluid, including a channel through which the fluid flows, a first inspection module arranged in a first region of the channel, and a second inspection module arranged in a second region of the channel, the device configured to provide a quantity that is representative of output of the second inspection module. The first inspection module is configured to measure at least one electrical property of the fluid passing through the first region. The second inspection module is configured to measure at least one optical property of the fluid passing through the second region. The inspection device also includes a controller connected to the first inspection module and to the second inspection module and configured to control the second inspection module according to the output of the first inspection module.

Подробнее
Номер записи: 26
12-12-2013 дата публикации

System and Method for Measuring Particles in a Sample Stream of a Flow Cytometer Using Low-Power Laser Source

Номер: US20130330763A1
Автор: Graves Steven W., Habbersett Robert C.
Принадлежит: Los Alamos National Security, LLC

A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals. 1. A particle interrogation system comprising:a flow chamber;a fluid container in connection with the flow chamber, the fluid container configured to deliver a gravity fed fluid to the flow chamber;a laser source configured to impinge a laser beam on one or more particles flowing in the flow chamber;a particle delivery system operably coupled to the flow chamber and configured to deliver a sample fluid containing one or more particles in the flow chamber,the particle delivery system being configured to provide a transit time of between at least about 100 microseconds to about 1 millisecond for a particle transported through the laser beam;at least one detector configured to receive one or more optical signals resulting from fluorescence or light scattered from the one or more particles; andsignal conditioning circuitry, operably coupled to the at least one detector.2. The system of claim 1 , wherein the laser source comprises a non-stabilized compact laser.3. The system of claim 1 , wherein the fluid container is connected to the flow chamber via a gravity fed sheath delivery line.4. The system of claim 1 , wherein the signal conditioning circuitry comprises a pre-amplifier stage coupled to the output of the ...

Подробнее
Номер записи: 27
26-12-2013 дата публикации

Optical particle detecting device and particle detecting method

Номер: US20130342838A1
Автор: Seiichiro Kinugasa
Принадлежит: Azbil Corp

An optical particle detecting device including a light source that emits an inspection light, a converting unit that converts the inspection light into collimated light, a focusing reflecting mirror that reflects toward a focal point the inspection light that has been converted into collimated light, a jet mechanism that causes an airstream including a particle to jet into the focal point of the focusing reflecting mirror, and a detecting portion that detects either scattered light or fluorescence produced by the particle included in the airstream being illuminated by the inspection light.

Подробнее
Номер записи: 28
06-02-2014 дата публикации

Methods of Formulating and Designing Liquid Drug Suspensions Containing Ion Exchange Resin Particles

Номер: US20140033806A1
Автор: Lockhart Daniel, MCMAHEN Russell, Sloane Michael, TENGLER Mark
Принадлежит: NEOS THERAPEUTICS, LP

The invention relates to the formulation and quality control of liquid drug suspensions. In particular, the invention relates to methods of formulating liquid suspensions comprising drug-containing resin particles. The invention also relates to methods of confirming the acceptability of drug-containing resin particles for use in formulating liquid drug suspensions. The invention further relates to methods of formulating liquid suspensions in which drug-containing resin particles, the liquid suspension, or both are modified to achieve a desired in vitro dissolution profile. The invention also relates to a novel dissolution method and methods of predicting in vivo bioequivalence based on in vitro dissolution methods. 1. A test method comprising(a) obtaining drug-resin complex particles;(b) combining said drug-resin complex particles with a plurality of excipients in a liquid carrier to form a liquid test drug suspension;(c) performing an in vitro dissolution assay to generate a test in vitro dissolution profile for the test drug suspension; and(d) comparing said test in vitro dissolution profile to a control in vitro dissolution profile generated by an in vitro dissolution assay for a control drug suspension, to determine whether the test in vitro dissolution profile matches the control in vitro dissolution profile,wherein said test drug suspension and said control drug suspension are substantially similar in physicochemical characteristics to a finally formulated suspension, andfurther wherein said control in vitro dissolution profile correlates to a target in vivo profile of a controlled release liquid suspension drug product.2. (canceled)3. A method for determining the acceptability of drug-resin complex particles for use in a controlled release liquid suspension drug suspension product , comprising(a) obtaining a sample of said drug-resin complex particles;(b) combining said sample with a plurality of excipients in a liquid carrier to form a liquid test drug ...

Подробнее
Номер записи: 29
20-02-2014 дата публикации

MEASUREMENT OF SERUM LIPOPROTEINS

Номер: US20140049775A1
Автор: KULKARNI Krishnaji R.
Принадлежит: Atherotech, Inc.

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry. 1. A method of measuring lipoprotein particle number in a sample from a subject , the method comprising:a. separating a first lipid fraction in the sample;b. obtaining a measurement of light scattering in the first lipid fraction; andc. calculating a particle count for the first lipid fraction that is a function of the measurement of light scattering.2. The method of further comprising:{'sup': 'th', 'a. separating a second, third to nlipid fraction in a sample;'}b. obtaining a measurement of light scattering for a least one lipid fractions; andc. calculating a particle count for at least one lipid fractions, wherein the particle count is function of the measurement of light scattering.3. The method of claim 1 , wherein the first lipid fraction is an LDL fraction.4. The method of claim 2 , wherein the at least one lipid fraction is an LDL fraction.5. The method of claim 1 , wherein the function is an approximately linear function.6. The method of claim 1 , wherein the separating step is accomplished using centrifugation.7. The method of claim 6 , wherein the sample is contained in a tube and the tube is sampled from the bottom so as to collect the fractions in descending order of density.8. A method of measuring lipoprotein particle number in a sample from a subject claim 6 , the method comprising:a. separating at least an HDL fraction, an LDL, fraction, and a VLDL fraction in a sample;b. obtaining a measurement of the light scattering from the LDL fraction; andc. transforming the ...

Подробнее
Номер записи: 30
13-03-2014 дата публикации

Methods and means for manipulating particles

Номер: US20140073000A1
Автор: Dong Sun, Xiaolin Wang
Принадлежит: City University of Hong Kong CityU

The present invention is concerned with a system for sorting target particles from a flow of particles. The system has a microscope, a light source, a CCD camera, microfluidic chip device with microfluidic channels, a detection apparatus for detecting the target particles with predefined specific features, a response generating apparatus for generating a signal in response to the detection of the target particles, and an optical tweezing system for controlling movement of optical traps, the optical tweezing system is operably linked to the response signal.

Подробнее
Номер записи: 31
20-03-2014 дата публикации

Nozzle assembly for a flow cytometer system and methods of manufacture

Номер: US20140076986A1
Автор: Johnathan Charles Sharpe, Kenneth Michael Evans, Kris BUCHANAN
Принадлежит: INGURAN LLC

A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.

Подробнее
Номер записи: 32
27-03-2014 дата публикации

PARTICLE SAMPLING AND MEASUREMENT IN THE AMBIENT AIR

Номер: US20140083167A1
Автор: Li Lin, Liu Benjamin Y. H., Marple Virgil A., Romay Francisco
Принадлежит: MSP Corporation

An apparatus and method for sampling and measuring air born particulate matter includes an inlet for the particulate containing gas to enter. A mechanism then removes coarse particles larger than a selected size while permitting filtered particles of less than the selected size to pass through. A chamber containing a quartz crystal sensor permits the filtered particles that have passed through to deposit to create an output signal in response to the deposited particle mass. 1. An apparatus for measuring particles in a gas , said apparatus comprising:an inlet for said gas to enter:a mechanism to remove coarse particles larger than a selected size permitting smaller particles of less than the selected size to pass through; anda first chamber containing a quartz crystal sensor on which the particles of less than the selected size can deposit to create an output signal in response to a deposited particle mass.2. The apparatus of said chamber being maintained at a temperature sufficient to prevent vapor condensation on said sensor.3. The apparatus of said temperature being in the approximate range from 25° C. to 55° C.4. The apparatus of said apparatus including a mechanism to maintain gas flow through said chamber at a specific set-point value.5. The apparatus of said apparatus including a gas filter for collecting a particle sample for analysis.6. The apparatus of said gas filter including a mechanism to main a gas flow through said filter at a specific set-point value.7. The apparatus of said coarse particles being removed are larger than about 10 μm in equivalent aerodynamic diameter.8. The apparatus of including an additional coarse particle collector for removing particles larger than approximately 2.5 or approximately 1.0 μm in equivalent aerodynamic diameter.9. The apparatus of including a second chamber similar to said first chamber of for charging particles with ions generated in a corona discharge and depositing charged particles on a quartz crystal sensor to ...

Подробнее
Номер записи: 33
27-03-2014 дата публикации

MICROPARTICLE ANALYSIS APPARATUS AND MICROPARTICLE ANALYSIS METHOD

Номер: US20140087453A1
Автор: Tahara Katsutoshi
Принадлежит: SONY CORPORATION

There is provided a microparticle analysis apparatus including a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target. The light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light. 1. A microparticle analysis apparatus comprising:a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target,wherein the light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light.2. The microparticle analysis apparatus according to claim 1 , wherein the light detection unit switches to the high-pass filter when the predetermined frequency is equal to or higher than 200 kHz.3. The microparticle analysis apparatus according to claim 2 , wherein the high-pass filter removes low frequency noise included in leakage light which avoids a zero-order light removal unit arranged between the microparticle and the light detection unit and which enters into the light detection unit.4. The microparticle analysis apparatus according to claim 3 , wherein the high-pass filter removes noise of a frequency lower than 2 kHz.5. The microparticle analysis apparatus according to claim 4 ,wherein the circuit includes a channel directly connected from an input side to an output side and a channel having the high-pass filter, andwherein the channel directly connected and the channel having the high-pass filter are connected in parallel.6. The microparticle analysis apparatus according to claim 5 , wherein the circuit includes a switch element to perform switching to one of the channel ...

Подробнее
Номер записи: 34
01-01-2015 дата публикации

System, Apparatus, and method for Measuring Surface Materials

Номер: US20150000375A1
Автор: George Nikolich, Vicken Etyemezian
Принадлежит: Desert Research Institute DRI

In one embodiment, the present disclosure provides a particulate monitoring system. The system includes a vehicle. A wake conditioner is mounted in the vehicle. A sample inlet is placed in communication with an engineered vehicle wake that will be produced by the wake conditioner when the vehicle is in motion.

Подробнее
Номер записи: 35
04-01-2018 дата публикации

DEVICES AND METHODS FOR FRACTIONATED PHOTOACOUSTIC FLOW CYTOMETRY

Номер: US20180000351A1
Автор: Zharov Vladimir Pavlovich
Принадлежит:

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams. The target objects have intrinsic photoacoustic contrast or may be labeled with photoswitchable or spaser-based probes. Fractioned beams may be used also for diagnostics with other spectroscopic methods (e.g., fluorescence, Raman or scattering) and energy sources both coherent and conventional such as lamp and LED in the broad spectral range from 10 Å to 1 cm (e.g., X-ray, UV, visible, NIR or microwaves) in continuous wave and pulse modes. 1. A fractionated photoacoustic flow cytometry system for the in vivo detection of target objects in a biofluid system of a living organism , comprising:a laser system comprising at least one laser comprising at least one wavelength for providing at least one laser beam to at least one target object within the biofluid system;a fractionated optical system configured to separate the at least one laser beam into fractionated laser beams having a spatial configuration on the skin above the biofluid system of the living organism; andan acoustic system comprising at least one focused ultrasound transducer for receiving more than one ...

Подробнее
Номер записи: 36
06-01-2022 дата публикации

ISOVOLUMETRIC SPHERING OF RED BLOOD CELLS

Номер: US20220003653A1
Автор: Hornig Gabriele, Matheis Karl-Philipp, Reichenwallner Ann-Kathrin, Richter Lukas, Ruhrig (deceased) Manfred, Schmidt Oliver, Ugele Matthias
Принадлежит:

An automatic analyzer for analyzing a medical probe includes an analysis cell for the probe, a piezo element, and an analysis device. The piezo element can be operated by applying a voltage and a frequency, and in the process an acoustic wave field is generated. A probe located in the analysis cell is located in the acoustic wave field when the piezo element is being operated. 1. An automatic analyzer for analyzing a medical sample , the analyzer comprising an analysis cell for the sample , a piezo element , and an analysis device , wherein the piezo element can be operated by application of a voltage and a frequency and generates an acoustic wave field , wherein a sample situated in the analysis cell is situated in the acoustic wave field during operation of the piezo element.2. The automatic analyzer as claimed in claim 1 , wherein a sample situated in the analysis cell is heated by the wave field during operation of the piezo element to at least 48 degrees Celsius.3. The automatic analyzer as claimed in claim 1 , wherein the analysis cell is a flow cell.4. The automatic analyzer as claimed in claim 3 , wherein the sample moves within the analysis cell at a flow rate between 0.002 and 5 mm per second.5. The automatic analyzer as claimed in claim 1 , wherein the analysis device comprises an optical microscope for analysis of a sample situated in the analysis cell.6. The automatic analyzer as claimed in claim 5 , wherein the microscope comprises a 20× to 60× objective.7. The automatic analyzer as claimed in claim 1 , wherein a sample is situated in the analysis cell and the sample comprises human or animal cells.8. A method for sphering a cell of a medical sample comprising:providing the cell situated in an analysis cell; andgenerating an acoustic wave field using a piezo element operated at a voltage and a frequency wherein the cell is situated in the wave field and is heated and thereby sphered by the wave field.9. The method as claimed in claim 8 , wherein the ...

Подробнее
Номер записи: 37
06-01-2022 дата публикации

Information processing apparatus, information processing method, and program

Номер: US20220003656A1
Автор: Junichiro Enoki, Kenji Yamane, Rei Murata
Принадлежит: Sony Group Corp

To provide an information processing apparatus, at least one non-transitory computer-readable storage medium, and a method which evaluate the appropriateness of a clustering result in consideration of characteristics of multidimensional data to be clustered. An information processing apparatus comprising: at least one hardware processor; and at least one non-transitory computer-readable storage medium storing processor-executable instructions that, when executed by the at least one hardware processor, cause the at least one hardware processor to perform: receiving multidimensional data obtained from a plurality of cells; clustering the multidimensional data to generate clustering results indicating a plurality of clusters including a first cluster and a second cluster that share at least a portion of the multidimensional data; and outputting information representing reliability of the clustering results, wherein the information is indicative of a relationship between the first cluster and the second cluster.

Подробнее
Номер записи: 38
06-01-2022 дата публикации

METHOD AND SYSTEM FOR CHARACTERIZING PARTICLES USING AN ANGULAR DETECTION IN A FLOW CYTOMETER

Номер: US20220003660A1
Автор: Giesecke-Thiel Claudia, Kage Daniel, Kaiser Toralf, von Volkmann Konrad
Принадлежит:

The invention relates to a method and system for characterizing particles using a flow cytometer comprising detecting radiated light from the particles using two or more detectors positioned to allow for the detection in two or more angular directions and generating a waveform, as a digital representation for the detected radiated light for each of said angulation direction. The waveforms are transformed using one or more basis functions to obtain one or more coefficients characterizing the waveform. The one or more coefficients characterizing the waveform preferably correspond to properties of the particle(s), thereby enabling analysis of physical properties of the particles (such as size, shape, refractive index) or biological properties of the particles (such as cell type, cell cycle state or localization or distribution of molecules within the cell and/or on the cell surface). In preferred embodiments the method and system are used for a label-free sorting of particles, in particular biological cells. 1. A method for characterizing particles using a flow cytometer comprising:a. passing of one or more particles in a fluid stream through a light beam of the flow cytometer,{'b': '3', 'b. detecting radiated light as one or more particles pass through the light beam using two or more detectors positioned to allow for the detection of the radiated light () in two or more angular directions,'}c. generating for each of the angular directions a waveform which is a digital representation of the detected radiated light for said angular direction, andd. transforming each waveform using one or more basis functions and obtaining one or more coefficients characterizing the waveform.2. Method according to claim 1 , wherein the detected radiated light is a forward scatter signal claim 1 , a side scatter signal and/or a fluorescence light and the two or more detectors are positioned to allow for detection of a forward scatter signal claim 1 , a side scatter signal and/or a ...

Подробнее
Номер записи: 39
03-01-2019 дата публикации

BIOLOGICAL DETECTION SYSTEM

Номер: US20190001320A1
Автор: Chen Ming, CHEN Sheng-Wen, Ho Hsin-Cheng, Hsu Wei-Cheng, HUANG CHUNG-ER
Принадлежит: CE Biotechnology, Inc.

A biological detection system for detecting a liquid sample containing a plurality of target biological particles includes a capturing device including a cell structure, an inlet, an outlet, a monolithic chip, and a layer of binding agent. The monolithic chip includes a substrate and a plurality of discrete nano-sized structures which are displaced from each other and each of which extends uprightly from the substrate to terminate at a top end. The layer of binding agent is formed on the top end of each of the discrete nano-sized structures for capturing the target biological particles. 1. A biological detection system adapted for detecting a liquid sample containing a plurality of target biological particles , comprising: [ [ a lower major surface, and', 'a lower adjoining surface opposite to said lower major surface in a transverse direction and having a lower recess which extends downwardly to terminate at an inner floor surface and which includes an upper sub-recess configured to permit passage of the liquid sample and a lower sub-recess, and, 'a lower cell body having'}, a top major surface, and', 'an upper adjoining surface which is opposite to said top major surface in the transverse direction and which has an upper recess extending toward said top major surface to terminate at a ceiling surface and configured to permit passage of the liquid sample;, 'an upper cell body having'}], 'a cell structure including'}, 'an inlet disposed upstream of said lower recess for introducing the liquid sample into said upper sub-recess of said lower recess;', 'an outlet disposed downstream of said lower recess and opposite to said inlet in a longitudinal direction;', 'a monolithic chip configured to be matingly fitted in said lower sub-recess and including a substrate and a plurality of discrete nano-sized structures which are displaced from each other in the longitudinal direction and each of which extends uprightly from said substrate to terminate at a top end; and', 'a ...

Подробнее
Номер записи: 40
07-01-2016 дата публикации

SELECTIVE CELL THERAPY FOR THE TREATMENT OF RENAL FAILURE

Номер: US20160002603A1
Автор: Atala Anthony, Yoo James J.
Принадлежит:

Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro. The kidney cells may include peritubular interstitial cells of the kidney, and preferably produce erythropoietin (EPO). The kidney cells may also be selected based upon EPO production. Methods of producing an isolated population of EPO producing cells are also provided, and methods of treating a kidney disease resulting in decreased EPO production in a patient in need thereof are provided, including administering the population to the patient, whereby the cells produce EPO in vivo. 17.-. (canceled)8. A method of producing an isolated population of erythropoietin (EPO) producing cells , said method comprising the steps of:providing differentiated kidney cells; andpassaging said differentiated kidney cells, wherein said cells produce EPO after said passaging;thereby producing an isolated population of EPO producing cells.9. The method of further comprising the step of selecting said differentiated kidney cells for EPO production.10. The method of claim 8 , wherein said passaging step comprises growth of differentiated kidney cells in a medium comprising insulin transferrin selenium (ITS).11. The method of claim 8 , wherein said differentiated kidney cells of said providing step further comprises endothelial cells of the kidney.12. The method of claim 8 , subject to the proviso that said population of EPO producing cells are not transfected with an exogenous DNA encoding a polypeptide.13. The method of claim 9 , wherein said selecting comprises selection based upon density and size.14. The method of claim 9 , wherein said selecting comprises the use of centrifugal gradients.15. The method of claim 8 , wherein said passaging is carried out from 1 to 20 times.16. The method of claim 8 , wherein said passaging is carried out at least 3 times.17. The method of claim 8 , wherein said population produces EPO without ...

Подробнее
Номер записи: 41
04-01-2018 дата публикации

PURIFICATION OF GERM STEM CELLS BY TARGETING MRP9

Номер: US20180002662A1
Автор: Bonkowski Michael S., Schultz Michael B., SINCLAIR DAVID A.
Принадлежит: President and Fellows of Harvard College

Provided herein are methods and compositions for the purification and detection of germ stem cells (e.g., oogonial stem cells) based on expression of MRP9. 1. A method for purifying a germ stem cell (GSC) , the method comprising:(a) contacting a sample comprising the GSC with an anti-MRP9 antibody that specifically binds to MRP9 and that does not specifically bind to DDX4;(b) incubating the sample under conditions such that the anti-MRP9 antibody forms a complex with an MRP9 protein expressed on the surface of the GSC; and(c) separating the GSC from other material present in the sample.2. The method of claim 1 , wherein the GSC is a oogonial stem cell (OSC).3. The method of claim 2 , wherein the sample is an ovarian tissue sample.4. The method of claim 3 , further comprising the step of obtaining the ovarian tissue sample from a subject.5. The method of claim 1 , wherein the GSC is a spermatogonial stem cell (SSC).6. The method of any one of to claim 1 , wherein the GSC is a human GSC.7. The method of any one of to claim 1 , wherein the anti-MRP9 antibody is monoclonal.8. The method of any one of to claim 1 , wherein the anti-MRP9 antibody is polyclonal.9. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically binds to an extracellular region of MRP9.10. The method of any one of to claim 1 , wherein the anti-MRP9 antibody does not specifically bind to an epitope having a sequence of APNPVDD.11. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically binds to an extracellular region of MRP9 having a sequence selected from the group consisting of SEQ ID NOs 5-20.12. The method of any one of to claim 1 , wherein the anti-MRP9 antibody specifically bind to an extracellular region of MRP9 having a sequence selected from the group consisting of SEQ ID NOs 6-12.13. The method of any one of to claim 1 , wherein the GSC is separated from other material present in the sample in step (c) by fluorescent activated cell sorting ...

Подробнее
Номер записи: 42
05-01-2017 дата публикации

COMPUTER-IMPLEMENTED METHOD OF ANALYSING DATA FROM MEASURED VALUES OF CHARACTERISTICS OF OBJECTS IN SAMPLES

Номер: US20170003212A1
Автор: BUYDENS Lutgarde, HILVERING Bart, JANSE Jeroen, KOENDERMAN Leo, VAN DEN BRINK Oscar
Принадлежит:

The invention relates to a computer implemented method of analysing data comprising measured values of characteristics of objects in samples, the data comprising —a first set of data (X) with measured values of characteristics of objects in reference samples; —a test set of data (X) with measured values of the characteristics of objects in a test sample; characterised by the method comprising; —fitting a control model to the first set of data to determine control loadings (P) each representing an independent correlation between characteristics; —projecting the first set of data (X) onto the control loadings (P) for determining a first set control scores (T) and determining one or more confidence intervals for the first set of control scores (T); —projecting the test data onto the control loadings (P) for determining test control scores; —determining if the test control scores are within one or more the confidence intervals. 117.-. (canceled)19. Computer implemented method according to claim 18 , wherein the step of fitting a control model comprises using the computer to conduct a principal component analysis to determine the control loadings (P).20. Computer implemented method according to claim 18 , wherein the first set of data (X) is centered per sample by using a computer for determining per reference sample (i) the mean value (m) of each characteristic and subtracting for each reference sample (i) the corresponding mean value (m) of the characteristic from the measured value in the first set of data (X) of the corresponding characteristic before fitting the control model.21. Computer implemented method according to claim 18 , wherein the test set of data (X) is centered per sample by using a computer for determining per test sample (i) the mean value of each characteristic and subtracting for each test sample (i) the corresponding mean value of the characteristic from the measured value in the test set of data of the corresponding characteristic before ...

Подробнее
Номер записи: 43
05-01-2017 дата публикации

Analysis Device and Analysis Method

Номер: US20170003213A1
Автор: HASEGAWA YUICHI, ITONAGA Makoto, ONO Masayuki, TSUJITA Koji, YAGYU Shingo
Принадлежит:

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which analytes and particles for labeling the analytes are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the analytes and determine that an analyte count is one when the pulse detector consecutively detects two pulse waves each having a pulse width less than a first reference value.2. The analysis device according to claim 1 , wherein the counting unit determines that the analyte count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less than a second reference value.3. The analysis device according to claim 1 , wherein claim 1 , when the pulse detector detects a first pulse wave having a pulse width less than the first reference value claim 1 , detects a second pulse wave after the first pulse wave claim 1 , and a pulse interval between the first pulse wave and the second pulse wave is greater than or equal to a third reference value claim 1 , the counting unit does not implement counting processing with regard to both the first pulse wave and the second pulse wave.4. The analysis ...

Подробнее
Номер записи: 44
05-01-2017 дата публикации

SHEATH FLUID SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES

Номер: US20170003273A1
Автор: Adams Thomas H., Farrell Gregory A., Groner Warren, Wanders Bart J., Zhao Xiaodong
Принадлежит:

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. 1. A particle and intracellular organelle alignment liquid (PIOAL) for use in a combined viscosity and geometric hydrofocusing analyzer , the PIOAL directing flow of a blood sample fluid of a given viscosity that is injected into a narrowing flowcell transition zone of the visual analyzer so as to produce a sample fluid stream enveloped by the PIOAL , the PIOAL comprising:a fluid having a higher viscosity than the viscosity of the blood sample fluid,a pH adjusting agent, andProcaine HCl,wherein a viscosity hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the viscosity difference, in combination with a geometric hydrofocusing effect induced by an interaction between the PIOAL fluid and the sample fluid associated with the narrowing flowcell transition zone, is effective to provide a target imaging state in at least some of the plurality of particles at an imaging site of the visual analyzer while a viscosity agent in the PIOAL retains viability of cells in the sample fluid stream leaving structure and content of the cells intact when the cells extend from the sample fluid stream into the flowing sheath fluid, andwherein the viscosity agent of the sheath fluid comprises glycerol at a concentration between about 1 to about 50% (v/v).2. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises polyvinylpyrrolidone (PVP).3. The PIOAL of claim 2 , wherein the polyvinylpyrrolidone (PVP) is at a concentration of 1% (w/v).4. The PIOAL of claim 1 , wherein the viscosity agent of the sheath fluid comprises glycerol at a concentration of 5% (v/v) and polyvinylpyrrolidone (PVP) ...

Подробнее
Номер записи: 45
07-01-2016 дата публикации

PHOTODETECTION DEVICE

Номер: US20160003727A1
Автор: Tanaka Masaki
Принадлежит: SHARP KABUSHIKI KAISHA

A photodetection device has an optical module () that includes a light source (), an excitation optical system, and a detection optical system and that two-dimensionally and relatively scans a transparent stage () in a first sampling direction and a second sampling direction intersecting the first sampling direction. A scan length in the first sampling direction is longer than a scan length in the second sampling direction. A data sampling unit in the detection optical system performs sampling for a distance of a second sampling interval during scanning in the second sampling direction, and performs sampling for a distance of a first sampling interval shorter than the distance of the second sampling interval during scanning in the first sampling direction. An aperture () of the excitation optical system sets a size in the first sampling direction of a spot shape of excitation light from the light source () to be smaller than a size in the second sampling direction. 1. A photodetection device comprising:a light-transmitting transparent stage on which a detection object is placed;an excitation optical system that irradiates the detection object with excitation light emitted from a light source;a detection optical system that detects light emitted from a detection surface of the detection object placed on the transparent stage by irradiation with the excitation light;a data sampling unit included in the detection optical system to sample an intensity of the detected light at a predetermined set interval; andan optical module that includes the light source, the excitation optical system, and the detection optical system and that two-dimensionally and relatively scans the transparent stage in a first sampling direction of the data sampling unit and a second sampling direction intersecting the first sampling direction,wherein a scan length in the first sampling direction in the optical module is longer than a scan length in the second sampling direction,wherein the data ...

Подробнее
Номер записи: 46
07-01-2016 дата публикации

METHOD OF AND APPARATUS FOR ASCERTAINING THE SIZE OF PARTICLES

Номер: US20160003728A1
Автор: Hardalupas Yannis
Принадлежит:

A method of ascertaining the size of small particles is disclosed. The method includes the steps of: a) intersecting at least two light beams at an intersection volume; b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light; c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; and d) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume. 1. A method of ascertaining the size of small particles , the method including the steps of:a) intersecting at least two light beams at an intersection volume;b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light;c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; andd) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume.2. A method according to claim 1 , wherein the measured indication of the variation of phase difference with angular displacement includes an indication of the angular position of transitions between local maxima and minima of the phase difference.3. A method according to claim 2 , wherein the measured indication includes information ...

Подробнее
Номер записи: 47
07-01-2016 дата публикации

FLUIDIC FLOW CYTOMETRY DEVICES AND PARTICLE SENSING BASED ON SIGNAL-ENCODING

Номер: US20160003729A1
Автор: Chen Chun Hao Randy, Cho Sung Hwan, Lo Yu-Hwa, Tsai Shang-Feng
Принадлежит:

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements. 1. A particle sorter for sorting particles in a fluid , comprising:a structure having an input channel connected at an actuation area to a plurality of output channels, wherein the particles in the fluid flow through the input channel to the actuation area, and each particle travels from the actuation area to one of the plurality of output channels, anda piezoelectric actuator for causing a flow disturbance in the actuation area in response to a control signal, wherein the flow disturbance operates to direct a particle along a trajectory to one of the plurality of output channels which is different than the output channel to which the particle would travel without the flow disturbance.2. The particle sorter of claim 1 , wherein the structure includes at least one of a polymer substrate claim 1 , a polydimethylsiloxine (PDMS) substrate claim 1 , or a glass substrate.3. The particle sorter of claim 2 , wherein the piezoelectric actuator is permanently bonded via UV ozone treatment to the PDMS substrate.4. The particle sorter of claim 1 , wherein the piezoelectric actuator is integrated with the structure.5. The particle sorter of claim 1 , further comprising a driver for generating the control signal.6. The particle sorter of claim 1 , wherein the control signal is a voltage signal having a controlled magnitude and frequency.7. The particle sorter of claim 1 , wherein the detection unit comprises a bank of filters for detecting a signal from the particle.8. The particle sorter of claim 1 , wherein the piezoelectric actuator includes a contact layer for coupling the piezoelectric actuator to the structure claim 1 , and further includes a piezoelectric layer for generating a signal to cause the flow disturbance in response to the control signal.9. The ...

Подробнее
Номер записи: 48
03-01-2019 дата публикации

SYSTEM AND METHOD FOR DROPLET DETECTION

Номер: US20190002956A1
Автор: Carman George, Dzenitis John, GLADE David, Hobbs Steve, JR. Anthony J., Makarewicz, Oen Joshua, Pristinski Denis, Simonian Dmitri, Stumbo David P.
Принадлежит:

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel. 1. A method of droplet detection , the method comprising:generating a single-file stream of droplets in carrier liquid;combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid;directing the combined streams to a detection channel using a spacing channel that tapers toward the detection channel, wherein a distance between adjacent droplets is increased as such droplets travel along the spacing channel toward the detection channel; anddetecting a signal from droplets passing through the detection channel.2. The method of claim 1 , wherein the step of generating includes a step of passing droplets through an alignment region of a sample inlet channel claim 1 , wherein a taper of the alignment region arranges droplets in single file before reaching a channel junction claim 1 , and wherein the step of combining includes a step of combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid at the channel junction.3. The method of claim 2 , wherein the step ...

Подробнее
Номер записи: 49
04-01-2018 дата публикации

Microfluidic Sensing

Номер: US20180003611A1
Автор: DOMINGUE Chantelle, Giri Manish, McGuinness Nick, Sells Jeremy
Принадлежит: Hewlett-Packard Development Company, L.P.

A device including a microfluidic channel structure formed on a substrate and including a first channel and a fluid actuator within the microfluidic channel structure. A sense region within the first channel is to receive a fluid flow of target biologic particles for counting in a single file pattern, with the sense region having a volume on a same order of magnitude as a volume of a single one of the target biologic particles. 1. A biologic test chip comprising:a substrate;a microfluidic channel structure formed on the substrate and including a first channel;a fluid actuator within the microfluidic channel structure.a sense region within the first channel to receive a fluid flow of biologic particles on a one-at-a-time basis via operation of the fluid actuator, the sense region having a volume on a same order of magnitude as a volume of a single respective one of the biologic particles.2. The chip of claim 1 , wherein the sense region operates according to a volume fraction in which a ratio of the volume of each single biologic particle relative to the volume of the sense region is on an order of tenths.3. The chip of claim 2 , comprising:at least one impedance sensor generally coextensive within the sense region to count biologic particles passing through the sense region.4. The chip of claim 3 , wherein the biologic particles are subject to a dilution factor on the order of tens.5. The chip of claim 4 , wherein the channel structure provides a non-uniform flow portion to align the biological particles into a single file flow pattern through the sense region claim 4 , the non-uniform flow portion including at least one of:an exclusion structure upstream from the sense region to exclude biologic particles larger than the volume of the sense region; andan inlet including a progressively narrowing cross-sectional area in the downstream orientation.6. The chip of claim 5 , wherein the first channel generally defines a first cross-sectional area and the first channel ...

Подробнее
Номер записи: 50
04-01-2018 дата публикации

Phenotypic High-Content Assay to Evaluate Drugs

Номер: US20180003613A1
Автор: Conn P. Michael
Принадлежит:

The present invention includes a high throughput screen for an active agent for the treatment of comprising: plating cells at least one pathophysiologically relevant mislocated mutant form of a peroxisomal enzyme; adding a control and compound to each plate from a library of compounds; fixing the cells; contacting the cells with an agent that detects the mislocated mutant form of a peroxisomal enzyme; and imaging the cells in the wells. 1. A method of determining the effectiveness of one or more drug candidates to change the intracellular localization of a target molecule , the method comprising:(a) incubating the one or more drug candidates with a first subset of the cells, and a control agent with a second subset of the cells;(b) fixing and staining the first and second subset of cells, wherein the stain detects the target molecule;(c) generating images of the first and second subset of cells with a camera;(d) measuring the difference in the intracellular localization of the target molecule in the first as compared to a second subset of cells; and(e) determining if the drug candidate modifies the localization of the intracellular localization of the target protein, wherein if the candidate drug modifies the intracellular localization of the target protein when compared to the placebo it is an effective drug candidate.4. The method of claim 1 , further comprising the step of determining cell count claim 1 , nuclear intensity claim 1 , morphology and condensation.5. The method of claim 1 , wherein the localization changes from the cytosol or mitochondria to a peroxisome.6. The method of claim 1 , wherein a candidate drug is selected from at least one of 26-Deoxymonensin B claim 1 , nigericin claim 1 , salinomycin claim 1 , or active derivatives thereof.7. A method of determining the effectiveness of one or more candidate pharmacoperones to treat and/or prevent protein misfolding claim 1 , the method comprising:(a) incubating the one or more candidate pharmacoperones ...

Подробнее
Номер записи: 51
04-01-2018 дата публикации

MULTI-THREADED FLUID PARAMETER SIGNAL PROCESSING

Номер: US20180003614A1
Автор: Giri Manish, Majeeth Saleem Jaffer, Sait M. A. Shameed
Принадлежит: Hewlett-Packard Development Company, L.P.

A data receiver thread is continuously executed to receive in which signals indicating a fluid parameter. A predetermined time quantity of the signals is repeatedly buffered. Upon completion of the buffering of each predetermined time quantity of the signals, a data processing thread is initiated that executes on the just completed buffered predetermined time quantity of signals. Upon completion of each data processing thread, data from the just completed data processing thread is passed to a data plotting thread. Results of the data plotting thread are displayed on a portable electronic device while the data receiver thread is being executed. 1. A method comprising:(a) outputting signals from a sensor that indicate a fluid parameter;(b) continuously executing a data receiver thread in which the signals indicating the fluid parameter are received;(c) repeatedly buffering a predetermined time quantity of the signals;(d) upon completion of the buffering of each predetermined time quantity of the signals, initiating a data processing thread that executes on the just completed buffered predetermined time quantity of signals;(e) upon completion of each data processing thread, passing data from the just completed data processing thread to a data plotting thread; and(f) displaying results of the data plotting thread on a portable electronic device while the data receiver thread is being executed.2. The method of claim 1 , wherein the data receiver thread receives the signals at a rate of at least 500 kHz.3. The method of further comprising:transmitting first data from the data processing thread across a wide area network to a remote server;receiving second data from the remote server across the wide area network, the second data comprising results of an analysis of the first data by the remote server; andpresenting the second data on the portable electronic device.4. The method of claim 1 , wherein the fluid comprises blood.5. The method of claim 1 , wherein the data ...

Подробнее
Номер записи: 52
03-01-2019 дата публикации

DOWNHOLE LOCAL SOLID PARTICLES COUNTING PROBE, PRODUCTION LOGGING TOOL COMPRISING THE SAME AND SAND ENTRY INVESTIGATION METHOD FOR HYDROCARBON WELLS

Номер: US20190003303A1
Автор: ABBASSI Linda, Donzier Eric, TAVERNIER Emmanuel
Принадлежит: Openfield SA

A downhole local solid particles counting probe () for counting solid particles () in a fluid () present in a hydrocarbon well in production comprising: 1. A downhole local solid particles counting probe for counting solid particles in a fluid present in a hydrocarbon well in production comprising:an elongated and flexible protective tube defining an internal cavity terminating by a membrane wall defining a tip, the protective tube and the membrane wall isolating the internal cavity from the fluid of the hydrocarbon well, the protective tube and membrane wall are made of metal or metal alloy and have a thickness such as to resist to a downhole hydrocarbon well pressure;a passive acoustic sensor mounted inside the internal cavity, the passive acoustic sensor having a front side mechanically coupled on the membrane wall of the tip;wherein:a characteristic dimension of the passive acoustic sensor is similar to solid particles average characteristic dimension, ranging from 0.5 mm to 1.5 mm, and a characteristic dimension of the membrane wall defining the tip ranges from 1 mm to 2 mm;the passive acoustic sensor is arranged to detect acoustic waves generated by solid particles impacting the membrane wall defining the tip so as to resolve an individual impact from a single solid particle and to produce a signal representative of a count of solid particles.2. The probe of claim 1 , wherein the internal cavity is under a pressure ranging from 0 to 4 atm.3. The probe according to claim 1 , wherein the passive acoustic sensor has a disk shape claim 1 , and the protective tube claim 1 , internal cavity and membrane wall have a cylindrical shape.4. The probe according to claim 1 , wherein the passive acoustic sensor is a piezoelectric ceramic.5. The probe according to claim 1 , wherein the protective tube and the membrane wall are made of austenite nickel-chromium-based super-alloys.6. The probe according to claim 4 , wherein the piezoelectric ceramic comprises metallization ...

Подробнее
Номер записи: 53
02-01-2020 дата публикации

LASER SENSOR MODULE FOR PARTICLE DETECTION WITH OFFSET BEAM

Номер: US20200003673A1
Автор: Jutte Petrus Theodorus, SPRUIT Johannes Hendrikus Maria, VAN DER LEE ALEXANDER MARC
Принадлежит:

A laser sensor module for detecting a particle density of particles, which includes: a laser; a detector; and a mirror. The laser is arranged to emit a laser beam to the mirror. A movement of the mirror is arranged to redirect the laser beam. The laser beam is displaced with respect to a rotation axis of the mirror such that a focus region of the laser beam is moving with a velocity having components normal and parallel to the optical axis of the redirected laser beam such that an angle between the parallel and the normal velocity component is at least a threshold angle of 2°. The detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles. 1. A laser sensor module for detecting a particle density of particles with a size of less than 20 μm , wherein the laser sensor module comprises:a laser;a detector; anda mirror rotatable about a rotation axis,wherein the laser beam is focused to a focus region,wherein the laser is arranged to emit a laser beam to the mirror,wherein a movement of the mirror is arranged to dynamically redirect the laser beam,wherein a direction of the redirected laser beam defines an optical axis,wherein the laser beam is displaced with respect to the rotation axis of the mirror such that the focus region of the laser beam is moving with a velocity comprising components normal and parallel to the optical axis of the redirected laser beam such that an angle α between the parallel velocity component with the normal velocity component is at least a threshold angle of 2°, andwherein the detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles.2. The laser sensor module ...

Подробнее
Номер записи: 54
02-01-2020 дата публикации

PERSONAL AIR QUALITY MONITORING SYSTEM

Номер: US20200003674A1
Автор: GIANDOMENICO Adam, PARISEAU David
Принадлежит:

An airborne, gas, or liquid particle sensor with multiple particle sensor blocks in a single particle counter. Each sensor would sample a portion of the incoming airstream, or possibly a separate airstream. The various counters could be used separately or in concert. 1. A personal particle counter apparatus , comprising:a power source in a device housing;an air intake and outlet fluidly connected to at least one chamber in the device housing;at least one light source that passes through the at least one chamber;at least one photo-detector within the at least one chamber, the at least one photo-detector configured to detect airborne particulates passing through the beam in air traversing from the air intake to the outlet;at least one amplifier in communication with the at least one photo-detector, the at least one amplifier configured to convert signals from the at least one photo-detector into amplified electrical pulses;at least one threshold comparator configured to process the amplified electrical pulses from the at least one amplifier and produces at least one output based on any pulse above a threshold pulse height that is a peak voltage of the amplified electrical pulse, the threshold associated with at least one particulate size channel;at least one microcontroller configured to process the at least one output from the at least one threshold comparator related to the at least one particulate size channel to count particles that pass through the chamber;and at least one output mechanism or storage device configured to communicate with with the at least one microcontroller to perform, at least one of, store and communicate the collected information.2. The personal particle counter apparatus of wherein the apparatus further comprises:an air flow device within the device housing.3. The personal particle counter apparatus of wherein the airflow device further comprises:a fan or blower configured to draw or push air through a beam of light in the chamber.4. The ...

Подробнее
Номер записи: 55
07-01-2021 дата публикации

Information processing apparatus and method and system for particle simulation

Номер: US20210003493A1
Автор: Kohei Hatanaka, Masaki Kazama, Tamon SUWA
Принадлежит: Fujitsu Ltd

Technique includes acquiring first contact data of first time, associated with first particle in first region; calculating first position data on particles in the first region at second time, and receiving second position data on particles in second region at the second time; detecting second particle being in contact with the first particle and in the first region at the first time and being in the first region at the second time; copying, when the first and second particles are in contact at the second time, displacement of the second particle from the first contact data to second contact data of the second time; detecting third particle being in the first or second region at the second time and in contact with the first particle; and copying, when the third particle is listed in the first contact data, displacement of the third particle to the second contact data therefrom.

Подробнее
Номер записи: 56
07-01-2021 дата публикации

Fine particle measurement device

Номер: US20210003494A1
Автор: Akinori Kimura, Asako MOTOMURA, Hiroshi Suganuma, Yoko Sugiyama
Принадлежит: Sumitomo Electric Industries Ltd

A fine particle measurement device includes a support stand (20) that has a groove (F) extending in a predetermined direction and is configured to support in the groove an observation container (10), which has an elongate shape and accommodates a liquid sample containing a fine particle therein such that an extending direction of the groove (F) coincides with a longitudinal direction of the observation container (10); and an imaging unit (40) that is configured to capture an image of the fine particle in the observation container (10) at a position where the support stand is out of a field of view, the observation container (10) being supported by the support stand (20).

Подробнее
Номер записи: 57
13-01-2022 дата публикации

MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY

Номер: US20220010351A1
Автор: CICHOCKI Nicolas, HÜBSCHMANN Thomas, MÜLLER Susann, OVERMANN Jörg
Принадлежит:

The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/− affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data. 1. Microbial Cytometric Mock Community for use in flow cytometric analysis , the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio , wherein the at least three different microbial species are selected such that , when measured using flow cytometry , the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community , preferably the at least three different microbial species differ in overall DNA content , relative genomic GC-content , average cell size , Gram +/− affiliation and/or capacity to form spores.2. Microbial Cytometric Mock Community of claim 1 , wherein the at least three different microbial species comprise or consist of species derived from archaea claim 1 , bacteria claim 1 , fungi claim 1 , protozoa and algae claim 1 , preferably derived from bacterial species.3. Microbial Cytometric Mock ...

Подробнее
Номер записи: 58
03-01-2019 дата публикации

Particle Detection Methods and Systems for Practicing Same

Номер: US20190003952A1
Автор: Hulett, III Frederic M., Shah Amish P.
Принадлежит:

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided. 123-. (canceled)24. A method for detecting cells in a flow cytometer , comprising:flowing a cellular sample comprising cells through a flow cell of a flow cytometer;detecting optical signals from the cells flowing through the flow cell at a first gain setting; anddetecting optical signals from the cells flowing through the flow cell at the second gain setting, wherein the second gain setting is different from the first gain setting.25. The method according to claim 24 , wherein the second gain setting is greater than the first gain setting.26. The method according to claim 25 , wherein the second gain setting is to account for the cellular sample having a high cell concentration.27. The method according to claim 24 , wherein the second gain setting is less than the first gain setting.28. The method according to claim 27 , wherein the second gain setting is to account for the cellular sample having a low cell concentration.29. The method according to claim 24 , wherein the first and second gain settings comprise a photo diodes gain setting claim 24 , a photo multiplier tubes (PMT) gain setting claim 24 , or both.30. The method according to claim 24 , wherein the cellular sample is a blood sample.31. The method according to claim 24 , comprising:analyzing the optical signals detected at the first gain setting to detect a first cell type; andanalyzing the optical signals detected at the second gain setting to detect a second cell type.32. The method according to claim 31 , wherein the first gain setting is higher than the second gain setting claim 31 , and wherein platelets are detected at the ...

Подробнее
Номер записи: 59
13-01-2022 дата публикации

Point-of-care medical diagnostic analyzer and devices, systems, and methods for medical diagnostic analysis of samples

Номер: US20220011328A1
Автор: Anne M. Leavitt, Bailey R. Auspland, Brady D. Jacques, David L. Connolly, David L. White, Dragan VIDACIC, Jared O. Ruehr, Jason J. Aguiar, John H. McGibbon, Jonathan W. Lawrence, Mark R. Dumont, Nicholas P. Prince, Richard Andrews, Samuel F. Clift
Принадлежит: Idexx Laboratories Inc

An analyzer having an inner chassis surrounded by a housing includes sample and dilution probes, a mixing housing including first and second mixing chambers, a flow cytometer including a flow cell, and sample and sheath pumps configured to perform first and second pluralities of tasks, respectively. The first plurality of tasks includes: aspirating sample into the sample probe, dispensing sample from the sample probe into the first and second mixing chambers, delivering first sample-dilution fluid mixture to the flow cell, and delivering second sample-dilution fluid mixture to the flow cell. The second plurality of tasks includes: dispensing sheath to the flow cell in cooperation with the delivery of the first sample-dilution fluid mixture to the flow cell, and dispensing sheath to the flow cell in cooperation with the delivery of the second sample-dilution fluid mixture to the flow cell.

Подробнее
Номер записи: 60
02-01-2020 дата публикации

Methods and Systems for Assessing Histological Stains

Номер: US20200005459A1
Автор: Berezhna Svitlana Y., Coleman Marilou L., Cowart Willie J., LI Wenjing, Nieves Alicea Rene, Olah Ema C., Stonas Walter G.
Принадлежит:

The present disclosure includes methods of assessing a histologically stained specimen based on a determined color signature of a region of interest of the specimen. Such assessments may be performed for a variety of purposes including but not limited to assessing the quality of the histological stain, as part of identifying one or more biologically relevant features of the image, as part of differentiating one feature of the image from other features of the image, identifying an anomalous area of the stained specimen, classifying cells of the specimen, etc. Also provided are systems configured for performing the disclosed methods and computer readable medium storing instructions for performing steps of the disclosed methods. 167-. (canceled)68. A system for assessing a histologically stained specimen , the system comprising:a) a microscope;b) a digital color camera attached to the microscope and configured to obtain a digital color image of the specimen;c) a library comprising a plurality of reference color signatures specific to biological features of histologically stained reference specimens;d) image processing circuitry configured to:i) define on the digital color image a region of interest (ROI) based on a biological feature of the specimen;ii) separate the digital color image into individual color channels; andiii) determine a color signature for the ROI, wherein the color signature comprises quantification of one or more color parameters over the ROI for one or more of the individual color channels; andiv) compare the determined color signature to one or more reference color signatures of the plurality of reference color signatures of the library to assess the histologically stained specimen.69. The system of claim 68 , wherein the system comprises a single memory connected to the image processing circuitry that stores the library and is configured to receive the digital color image.70. The system of claim 68 , wherein the system comprises a first memory ...

Подробнее
Номер записи: 61
20-01-2022 дата публикации

DETECTION METHOD AND DETECTION DEVICE

Номер: US20220018753A1
Автор: ARIMOTO Satoshi
Принадлежит:

A target substance detection method includes forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance (for example, an antibody) having a property of specifically binding to the target substance; subjecting a bound particle and an unbound particle to dielectrophoresis in a liquid, the bound particle being the dielectric particle constituting the complex, the unbound particle being a dielectric particle not constituting the complex; and detecting the target substance in the complex, based on a difference in motion between the bound particle and the unbound particle caused by the dielectrophoresis. 1. A detection method comprising:forming a complex by causing a target substance and a dielectric particle to bind to each other, the dielectric particle being modified with a substance having a property of specifically binding to the target substance;subjecting a bound particle and an unbound particle to dielectrophoresis in a liquid, the bound particle being the dielectric particle constituting the complex, the unbound particle being a dielectric particle not constituting the complex; anddetecting the target substance included in the complex, based on a difference in motion between the bound particle and the unbound particle caused by the dielectrophoresis, whereina rotating electric field is produced in the liquid to subject the bound particle and the unbound particle to the dielectrophoresis,the bound particle moves to draw a circular path by the dielectrophoresis, andthe unbound particle rotates around an axis passing through a center position of the unbound particle by the dielectrophoresis.2. The detection method according to claim 1 , whereinthe dielectric particle includes a fluorescent substance, andin the detecting of the target substance, the liquid is irradiated with excitation light and fluorescence emitted by the fluorescent substance included in each of the bound ...

Подробнее
Номер записи: 62
20-01-2022 дата публикации

METHOD FOR CHARACTERISING A PARTICLE ON THE BASIS OF A HOLOGRAM

Номер: US20220018756A1
Автор: Allier Cédric, BLANDIN Pierre, CIONI Olivier, Dinten Jean-Marc, Herve Lionel, Joly Pierre
Принадлежит:

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest. 117-. (canceled)18. A method for characterizing a particle within a sample , the sample lying between an image sensor and a light source , the image sensor lying in a detection plane , the method comprising:a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis;b) acquiring an image of the sample with the image sensor, the image comprising a plurality of elementary diffraction patterns, each elementary diffraction pattern corresponding to one particle;c) on the basis of the acquired image, reconstructing a complex image representative of a complex amplitude of the exposure light wave on at least one reconstruction surface passing through the sample, the reconstruction being achieved by implementing an iterative reconstruction algorithm, the algorithm comprising, in each iteration, updating a phase of the exposure light wave in the detection plane or on the reconstruction surface;d) selecting a region of interest of the complex image, the selected region of ...

Подробнее
Номер записи: 63
11-01-2018 дата публикации

FLUID PUMPING AND TEMPERATURE REGULATION

Номер: US20180008979A1
Автор: Giri Manish, Sait M A Shameed, Smith Matthew David, YU Joshua M.
Принадлежит: Hewlett-Packard Development Company, L.P.

Fluid may be pumped within a microfluidic channel across a cell/particle sensor using a microscopic resistor. The microscopic resistor may be selectively actuated so as to heat the fluid within the microfluidic channel to a temperature below a nucleation energy of the fluid so as to regulate a temperature of the fluid for at least when the cell/particle sensor is sensing the fluid. 1. An apparatus comprising:a microfluidic channel to receive a fluid;an analyte sensor within the microfluidic channel;a microscopic resistor in the microfluidic channel; and actuate the microscopic resistor to a fluid pumping state in which fluid adjacent the microscopic resistor is heated to a temperature above a nucleation energy of the fluid to pump the fluid across the cell/particle sensor; and', 'selectively actuate the microscopic resistor to a temperature regulating state in which fluid adjacent the microscopic resistor is heated to a temperature below the nucleation energy of the fluid, wherein the controller is to selectively actuate the microscopic resistor to the temperature regulating state to regulate a temperature of the fluid for at least when the analyte sensor is sensing the fluid., 'a controller to2. The apparatus of further comprising a temperature sensor to output temperature signals indicative of a temperature of the fluid claim 2 , wherein the controller is to selectively actuate the microscopic resistor to the temperature regulating state based upon the temperature signals.3. The apparatus of comprising:a cassette containing a microfluidic diagnostic chip, the microfluidic diagnostic chip comprising the microfluidic channel, the temperature sensor and the microscopic resistor; anda portable electronic device containing the controller, wherein the cassette is releasably connectable to the portable electronic device.4. The apparatus of claim 1 , wherein the controller is to selectively actuate the microscopic resistor so as to apply different amounts of heat when in ...

Подробнее
Номер записи: 64
14-01-2021 дата публикации

Cell capture in microfluidic devices

Номер: US20210008554A1
Автор: Johan ELF, Martin Lovmar, Mikael Olsson, Ove Ohman, Ozden Baltekin
Принадлежит: Astrego Diagnostics AB

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel (30, 60) of an upstream microfluidic device (1). Target cells present in the biological sample are captured in cell channels (20) of the upstream microfluidic device(1). Once at least a minimum number of target cells are captured in the cell channels (20), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device (1) to release the target cells captured in the cell channels (20) of the upstream microfluidic device (1) and enable transfer the target cells into cell channels (120) of a downstream microfluidic device (100).

Подробнее
Номер записи: 65
27-01-2022 дата публикации

METHOD FOR DETECTING PARTICLES OR AEROSOL IN A FLOWING FLUID, COMPUTER PROGRAM, AS WELL AS ELECTRICAL MEMORY MEDIUM

Номер: US20220026338A1
Автор: Rusanov Radoslav
Принадлежит:

A method for detecting particles or aerosol in a flowing fluid, using the principle of laser-induced incandescence. The method includes the following steps: a. focusing a laser light originating from a laser in a spot; b. conducting a fluid which includes particles or aerosol through the spot; c. detecting a thermal radiation originating from the spot with the aid of a detector; and d. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, the duration of the time intervals being dependent on a velocity of the fluid. 114-. (canceled)15. A method for detecting particles or aerosol in a flowing fluid , using laser-induced incandescence , the method comprising the following steps:a. focusing a laser light originating from a laser in a spot;b. conducting the fluid which includes particles or aerosol through the spot;c. detecting a thermal radiation originating from the spot using a detector; andd. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, a duration of the time intervals being dependent on a velocity of the fluid.16. The method as recited in claim 15 , wherein at least several of the time intervals overlap.17. The method as recited in claim 16 , wherein the duration of the time intervals is greater than an expected full width at half maximum (FWHM) of the variable characterizing the thermal radiation.18. The method as recited in claim 17 , wherein the duration of the time intervals is 1 to 2 times the expected FWHM.19. The method as recited in claim 18 , wherein the duration of the time intervals is 1.5 times the expected FWHM.20. The method as recited in claim 16 , wherein an overlapping time period of the time intervals corresponds to at least half the duration of the time interval.21. The method as recited in claim 16 , wherein a particle is considered to be detected when the variable characterizing the thermal ...

Подробнее
Номер записи: 66
27-01-2022 дата публикации

METHODS AND DEVICES FOR EVALUATING PERFORMANCE OF A DIODE LASER

Номер: US20220026339A1
Автор: Amiri Waheed, Gianduzzo David J., Nguyen An-Dien
Принадлежит:

Methods for evaluating performance a diode laser are provided. In embodiments, methods include receiving a laser beam profile of a diode laser, determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile, computing a first ratio between the second and third laser beam widths, computing a second ratio between the first and second laser beam widths, evaluating laser performance based on the first and second ratios, and outputting a determination regarding the suitability of the laser for use in a flow cytometry setting. Devices for practicing the subject methods are also provided, and include first and second stages configured to receive a diode laser and beam profiler, respectively. Aspects of the invention further include flow cytometers incorporating a diode laser that has been evaluated by the subject method. 1. A method for evaluating performance of a diode laser , the method comprising:receiving a laser beam profile of the diode laser, the laser beam profile comprising laser beam width data and laser intensity data;determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile;computing:a first ratio between the second and third laser beam widths; anda second ratio between the first and second laser beam widths; andevaluating laser performance based on the first and second ratios.2. The method according to claim 1 , wherein the diode laser is a semiconductor laser diode.35-. (canceled)6. The method according to claim 1 , wherein evaluating performance of the diode laser comprises assessing the extent to which the laser beam profile deviates from a Gaussian beam shape.7. The method according to claim 6 , wherein assessing the extent to which the laser beam profile deviates from a Gaussian beam shape comprises identifying whether multiple modes are present in the laser beam profile.8. The method according to ...

Подробнее
Номер записи: 67
12-01-2017 дата публикации

METHOD FOR DETERMINING A CONCENTRATION OF LIPIDS IN A MICROORGANISM

Номер: US20170010200A1
Автор: Allier Cédric, BOUTET Jerome, FINAZZI Giovanni, MARECHAL Eric
Принадлежит: Commissariat A L'Energie Atomique Et Aux Energies Alternatives

The invention relates to a method for determining a concentration of lipids in a microorganism, in particular a micro-alga, wherein: 21414. The method according to claim 1 , characterized in that a zone of interest is defined such that it comprises a single unit diffraction pattern (A; B).3132323131. The method according to claim 1 , characterized in that a zone of interest (; A; B) is defined such that it comprises a plurality of unit diffraction patterns () claim 1 , and in that a mean concentration of lipids in the microorganisms of interest is determined.4. The method according to claim 1 , characterized in that the numerical indicator (In) consists of a standard deviation of the light intensity relative to a reference light intensity.5145145146146. The method according to claim 1 , characterized in that the numerical indicator (In) consists claim 1 , for each unit diffraction pattern claim 1 , of a difference between the light intensity (A; B) in the center of said unit diffraction pattern and the light intensity (A; B) of the first dark ring thereof.6680. The method according to claim 1 , characterized in that it is implemented for performing an in situ follow up of a culture of microorganisms in a pool or a bioreactor (). The present invention relates to the field of methods for determining a concentration of lipids in a microorganism, in particular micro-algae.The invention also relates to a device for implementing such a method.A micro-algae is a microscopic organism, for example of the unicellular type, and the size of which typically ranges from a few micrometers to a few hundreds micrometers. Micro-algae typically contain photosynthetic organisms living essentially in water. The micro-algae have an interest in particular in the field of biofuels, because of their lipid storage properties, these lipids being then apt be used for producing fuels.In comparison with the traditional biofuel sources, micro-algae have many advantages, from which a very quick ...

Подробнее
Номер записи: 68
12-01-2017 дата публикации

Cross-Instrument Method and System for Cell Population Discrimination

Номер: US20170010204A1
Автор: Gutierrez William H., QIAN Cheng, Riley John S.
Принадлежит:

The present invention provides methods and systems to combine the capabilities of a hematology analyzer with those of a flow cytometer to yield a far more powerful analytical system than either device alone. In one embodiment, a method of analyzing a cell sample includes receiving a first data generated by an analysis of a first aliquot of the sample on a first particle analyzer having a fluorescence measurement device such as a flow cytometer, detecting at least one unresolved cell population in the first data, and accessing a second data stored on a storage device wherein the second data was previously generated by interrogating a second aliquot of the sample using at least one of a cell volume measurement device and a cell conductivity measurement device in a second particle analyzer such as a hematology analyzer. The unresolved cell population in the first data is then resolved using the second data. Corresponding system embodiments are also disclosed. 121-. (canceled)22. A system for analysis of a blood cell suspension sample , comprising:a first particle analyzer configured to receive a first aliquot of the blood cell suspension sample as input and to produce a first data using a fluorescence measurement device, wherein the first data includes a cell population of at least one of a first cell type and a second cell type, wherein there is substantial overlap in location of the first cell type and the second cell type in a plot of the first data;a second particle analyzer configured to receive a second aliquot of said sample as input and to produce a second data using at least one of a cell volume measurement device, a light scatter measurement device, and a cell conductivity measurement device and wherein there is substantial overlap in location of the first cell type and the second cell type in a plot of the second data; anda computer configured to receive the first data and the second data to automatically resolve an unresolved cell population in the first ...

Подробнее
Номер записи: 69
14-01-2016 дата публикации

Methods and Apparatus for Real-Time Detection and Clearing of a Clog

Номер: US20160011094A1
Автор: Aaron B. Kennington
Принадлежит: Intellicyt Corp

A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.

Подробнее
Номер записи: 70
14-01-2016 дата публикации

Method and device for examining myocardial toxicity and evaluating cardiomyocytes

Номер: US20160011176A1
Автор: Fumimasa Nomura, Kenji Yasuda, Tomoyuki Kaneko
Принадлежит: Tokyo Medical and Dental University NUC

Regarding cardiomyocytes and fibroblasts, it is meaningful to develop a device or system whereby, upon the transmission of pulsation from an adjacent cardiomyocyte or fibroblast, cell potential and cell morphology can be accurately measured on a single cell basis and the toxicity of a drug on cardiomyocytes can be examined on the basis of accurately measured cell potential and cell morphology of a single cell. In the present invention, a mass of cardiomyocytes is disposed on a transparent substrate and the qualities of the cardiomyocytes are evaluated depending on the response of the cardiomyocytes to a forced pulsation stimulus that is applied to the pulsating cardiomyocytes. A mass of cardiomyocytes, said mass being disposed on the transparent substrate, is exposed to a flow of a drug-containing liquid so as to allow the drug to act on cells configuring a network. The level of myocardial toxicity of the drug is evaluated by measuring the fluctuations that are obtained by comparing adjacent pulsating cardiomyocytes in the network.

Подробнее
Номер записи: 71
11-01-2018 дата публикации

OPTICAL PARTICLE SORTER

Номер: US20180010997A1
Автор: CURRY JOHN J., LEVINE ZACHARY H.
Принадлежит:

A process for optically sorting a plurality of particles includes: providing a particle receiver; producing particles; receiving the particles by the particle receiver; receiving a light by the particle receiver; producing a standing wave optical interference pattern in an optical interference site of the particle receiver from the light; subjecting the particles to an optical gradient force from the standing wave optical interference pattern; deflecting the particles into a plurality of deflected paths to form the sorted particles from the particles; and propagating the sorted particles from the optical interference site through the deflected paths to optically sort the particles 1. An optical particle sorter comprising: a particle entrance that receives a plurality of particles;', 'an optical entrance that receives light and that is geometrically disposed at a non-parallel angle with respect to the particle entrance;', 'a sorted particle exit opposing the particle entrance and that communicates sorted particles from an optical interference site; and', 'the optical interference site interposed between the particle entrance and the sorted particle exit;, 'a particle receiver comprising produces a first light; and', 'produces a standing wave optical interference pattern in the optical interference site of the particle receiver; and, 'a first light source in optical communication with the particle receiver and that provides the particles; and', {'b': '24', 'communicates the particles to the particle receiver at an acute angle with respect to the standing wave optical interference pattern ,'}], 'a particle source in fluid communication with the particle receiver and thatwherein the optical particle sorter sorts the particles into a plurality of sorted particles that exit the particle receiver at the sorted particle exit, andthe sorted particles propagate in a plurality of deflected paths relative to a path of propagation of the particles at the particle entrance, the ...

Подробнее
Номер записи: 72
11-01-2018 дата публикации

EVALUATING BIOLOGICAL MATERIAL FOR UNASSOCIATED VIRUS-LIKE PARTICLES

Номер: US20180010999A1
Автор: Artinger Michael A., Gates Tyler Donald, Kohlmeier Francis Kevin, Olszowy Michael W.
Принадлежит:

A method for evaluating a biological material for unassociated virus-like particles virus size having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-like particle and fluorescent antibody stain. 2. A method according to claim 1 , wherein the epitope is selected from the group consisting of a baculovirus epitope claim 1 , an adenovirus epitope claim 1 , an influenza virus epitope claim 1 , an enterovirus epitope claim 1 , an adeno-associated virus (AAV) epitope and a norovirus epitope.3. A method according to claim 1 , wherein the epitope is a first epitope claim 1 , the flow cytometry is first flow cytometry claim 1 , the fluid sample is a first fluid sample including a first portion of the of the biological material sample claim 1 , the unassociated labeled particles are first unassociated labeled particles and the excitation radiation is first excitation radiation claim 1 , and the method comprises: flowing the second fluid sample through a flow cell of a flow cytometer;', 'subjecting the second fluid sample flowing through the flow cell to second excitation radiation, which is the same as or different than the first excitation radiation, capable of causing a second fluorescent emission response that is different than the first fluorescent emission response from the second fluorescent antibody stain; and', 'detecting radiation from the flow cell within a wavelength range of the fluorescent emission and evaluating the detected radiation to identify detection events indicative of passage through the flow cell of second unassociated labeled particles of virus size including a particle of virus size having the second ...

Подробнее
Номер записи: 73
11-01-2018 дата публикации

Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy

Номер: US20180011001A1
Автор: David G. Grier, Fook Chiong CHEONG, Ke Xiao
Принадлежит: New York University NYU

An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

Подробнее
Номер записи: 74
11-01-2018 дата публикации

SYSTEM AND METHOD FOR IMMERSION FLOW CYTOMETRY

Номер: US20180011016A1
Автор: Swalwell Jarred E.
Принадлежит: UNIVERSITY OF WASHINGTON

An immersion cytometry system () having a primary focusing optic immersed in a fluid stream () containing suspended particles (). The system includes a light source () configured to illuminate a sensing region in the fluid stream that includes a focus of the primary optic. Light scattered and/or fluoresced from suspended particles passing through the sensing region is focused by an external tube lens on an external detector. The primary optic in one embodiment is a ball lens. In some embodiments, one or more filter/beam splitters on the optical axis reflect a portion of the signal light towards corresponding detectors, each filter being configured to reflect a preselected waveband of light. 1. A flow cytometry system for detecting particles suspended in a fluid stream , the system comprising:a light source configured to direct light through a sensing region in the fluid stream;a primary focusing optic at least partially immersed in the fluid stream and positioned to receive and focus light scattered by the suspended particles when they are in the sensing region; anda tube lens positioned to receive light from the primary optic and to focus the received light onto a first photo detector, the primary optic and the tube lens defining an optical axis that is aligned with the photo detector;wherein the first photo detector is configured to generate a signal responsive to the light focused by the tube lens onto the first photo detector and to transmit the generated signal to a processing system.2. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic comprises a ball lens.3. The immersion flow cytometry system of claim 2 , wherein the ball lens comprises glass.4. The immersion flow cytometry system of claim 3 , wherein the glass is a flint glass.5. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic is configured to chromatically distort the light claim 1 , such that the location of the focus plane on the ...

Подробнее
Номер записи: 75
11-01-2018 дата публикации

METHOD, DEVICE, AND SYSTEM FOR THE AUTOMATED DETERMINATION OF OPTICAL DENSITIES OR OF THE CHANGE IN OPTICAL DENSITIES OF REACTION MIXTURES IN SHAKEN REACTORS

Номер: US20180011027A1
Автор: FRANK David, Herzog Konrad
Принадлежит:

The invention relates to a method, to a device, and to a system for the automated determination of optical densities or of the change in optical densities of reaction mixtures in shaken reactors during shaking operation. Methods and devices currently used therefor are often unreliable, are susceptible to environmental and process factors, or require interruptions to the shaking operation that impair the process control. The problem addressed by the invention is that of specifying a method and a device for the automated determination of optical densities or of the change in optical densities of reaction mixtures in shaken reactors during shaking operation that operate reliably under various environmental and process conditions. This problem is solved by means of a new measurement method, wherein the reaction mixture distribution, which periodically fluctuates because of the shaking action, is used to record measurement points () of transmission/scattered-light measurements, which measurement points fluctuate periodically as a result of shaking. All measurement points () of a measurement operation are combined into a measurement series (), from which the optical density and/or the change in the optical density, and other process parameters, can be determined with high reliability by means of suitable mathematical methods. The invention is suitable in particular for biotechnological, pharmaceutical, chemical, and biochemical screening and optimization and process-monitoring applications. 121. Method for determining the optical density and/or the change in the optical density of a reaction mixture in a shaken reactor , characterised in that{'b': 4', '2, 'light from at least one light source enters the reaction mixture , and'}{'b': 2', '5', '6, 'the light exiting the reaction mixture is detected by at least one light sensor /, and'}{'b': 5', '6', '1', '2, 'during the detection of the light by the at least one light sensor / the reactor and the reaction mixture are shaken ...

Подробнее
Номер записи: 76
11-01-2018 дата публикации

MICROFLUIDICS DETECTION

Номер: US20180011042A1
Автор: Domingue Chantelle E., Giri Manish, Sells Jeremy, Valencia Melinda M.
Принадлежит: Hewlett-Packard Development Company, L.P.

A method of microfluidic detection can include detecting, using an impedance sensor, an impedance of a fluid to indicate whether a threshold amount of fluid has been received in a reservoir of a microfluidic chip. The method can include initiating a test performed by the microfluidic chip on the received fluid when the threshold amount of fluid has been received. 1. A method , comprising:detecting, using an impedance sensor, an impedance of a fluid to indicate that a threshold amount of fluid has been received in a reservoir of a microfluidic chip; andwhen the threshold amount of fluid has been received, initiating a test performed by the microfluidic chip on the received fluid.2. The method of claim 1 , including detecting whether the threshold amount of fluid has been received in response to receiving the fluid in the reservoir.3. The method of claim 1 , including detecting claim 1 , using the impedance sensor claim 1 , a second impedance of the fluid to indicate that the threshold amount has not been received in the reservoir claim 1 , wherein the test is not initiated when the detection of the fluid indicates the threshold amount has not been received.4. The method of claim 1 , wherein detecting the impedance of the fluid comprises:measuring the impedance indicating the amount of the received fluid; andcomparing the amount of the received fluid to the threshold amount.5. The method of claim 1 , including detecting whether the fluid is a particular type of fluid using an additional impedance sensor.6. The method of claim 5 , wherein the test is initiated when the particular type of fluid is detected and the threshold amount has been received.7. A method claim 5 , comprising:receiving fluid in a reservoir of a microfluidic chip;measuring, using an impedance sensor, a value of a parameter of the fluid;comparing, by a processor, the measured value to a threshold value; andwhen the measured value exceeds the threshold value, indicating to the microfluidic chip to ...

Подробнее
Номер записи: 77
10-01-2019 дата публикации

LIGHT EXTINCTION TOMOGRAPHY FOR MEASUREMENT OF ICE CRYSTALS AND OTHER SMALL PARTICLES

Номер: US20190011347A1
Автор: Bencic Timothy J., Fagan Amy F., Izen Steven H., Maniyedath Arjun K., Rohler David P.
Принадлежит:

A tomography duct for wind tunnels includes a plurality of light sources and sensors displaced around a support structure. The light sources are cycled and sensor measurements are made from sensors opposite the light sources. Tomographic algorithms are used to determine an extinction map from the sensor measurements. The extinction map provides details about particles in a cross-section of the air flow through the tomography duct. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. (canceled)9. (canceled)10. (canceled)11. A method , comprising:measuring a dark current of a sensor element;emitting light from a light source across a tomography duct through which an air flow can be passed;receiving, by a sensor element positioned across the tomography duct, the emitted light;measuring on the sensor element an unextinguished light intensity of the emitted light;calibrating a tomography algorithm based at least in part on the dark current and the unextinguished light intensity;receiving a plurality of particles into the air flow;measuring on the sensor element an extinguished light intensity of the emitted light caused by the one or more of the plurality of particles in the air flow;reconstructing, using the calibrated tomography algorithm, an extinction map of a cross-section of the air flow based at least in part on the extinguished light intensity.12. The method of claim 11 , further comprising:generating the air flow; andinjecting the plurality of particles into the air flow.13. The method of claim 12 , wherein the plurality of particles is selected from the group consisting of water droplets claim 12 , supercooled water droplets claim 12 , and ice particles.14. The method of claim 11 , further comprising:adjusting the intensity of the light emitted from the light source based on the measured unextinguished light intensity.15. The method of claim 11 , further comprising:determining one or more characteristics of one or more ...

Подробнее
Номер записи: 78
10-01-2019 дата публикации

FINE PARTICLE MEASUREMENT APPARATUS, INFORMATION PROCESSING APPARATUS, AND INFORMATION PROCESSING METHOD

Номер: US20190011348A1
Автор: Tahara Katsutoshi
Принадлежит:

To provide a technology that an output level difference is corrected with high accuracy in fine particle measurement that optically measures properties of fine particles. The present technology provides a fine particle measurement apparatus including a detector that detects light from fluorescent reference particles that emit fluorescence having a predetermined wavelength bandwidth, and an information processor that specifies a relationship between an applied voltage coefficient corresponding to a feature amount of a predetermined output pulse and a control signal of the detector on the basis of a feature amount of an output pulse detected by the detector and the control signal of the detector at the time of detecting the feature amount of the output pulse, the feature amount of the output pulse being dependent on the control signal of the detector, or the like. 1. A fine particle measurement apparatus , comprising:a detector that detects light from fluorescent reference particles that emit fluorescence having a predetermined wavelength bandwidth; andan information processor that specifies a relationship between an applied voltage coefficient corresponding to a feature amount of a predetermined output pulse and a control signal of the detector on the basis of a feature amount of an output pulse detected by the detector and the control signal of the detector at the time of detecting the feature amount of the output pulse,the feature amount of the output pulse being dependent on the control signal of the detector.2. The fine particle measurement apparatus according to claim 1 , whereinthe detector includes a plurality of PMTs.3. The fine particle measurement apparatus according to claim 2 , whereinthere is an output difference among the plurality of PMTs.4. The fine particle measurement apparatus according to claim 1 , whereinthe feature amount of the output pulse is a height of the output pulse or an area of the output pulse.5. The fine particle measurement apparatus ...

Подробнее
Номер записи: 79
10-01-2019 дата публикации

LABEL-FREE CHARACTERIZATION OF PARTICLES SUSPENDED IN A FLUID

Номер: US20190011349A1
Автор: Bashir Rashid, DAMHORST Gregory, Durack Gary, GHONGE Tanmay, HASSAN Umer, JR. Bobby, Reddy
Принадлежит: The Board of Trustees of the University of Illinois

Provided are methods and systems that characterize a property of a particle suspended in a fluid sample in a label-free manner. Detection elements are provided fluidically adjacent upstream and downstream from a modulation element. Fluid sample containing particles flows across a first detection element and a first particle parameter detected for each particle that passes the first detection element or a first aggregate particle parameter for a plurality of particles that pass the first detection element. The particles flow from the first detection element to a first modulation element, wherein the first modulation element effects a change in a property of the particles flowing past the first modulation element. A second detection element then detects the particle parameter again or a second aggregate particle parameter for a plurality of particles that pass the second detection element. Comparing the first and second particle or aggregate parameters thereby characterizes the particle property. 1. A label-free method for characterizing a property of a particle suspended in a fluid sample , the method comprising the steps of:flowing a fluid sample containing particles across a first detection element, wherein the particles flow in substantially single file across the first detection element;detecting with the first detection element a particle parameter for at least a portion of the particles that pass the first detection element;flowing the particles from the first detection element to a first modulation element, wherein the first modulation element effects a change in the particle parameter of the particles flowing past the first modulation element;flowing the particles from the first modulation element across a second detection element, wherein the particles flow in substantially single file across the second detection element;detecting with the second detection element the particle parameter for the at least a portion of the particles that pass the second ...

Подробнее
Номер записи: 80
14-01-2021 дата публикации

Air bubble measurement device and air bubble measurement method

Номер: US20210010917A1
Автор: Shintaro Ishikawa
Принадлежит: SUMITOMO METAL MINING CO LTD

An air bubble measurement device is a device that measures the air bubbles moving in the liquid. The air bubble measurement device includes a measurement chamber that holds a liquid. The measurement chamber includes an introduction port to introduce the air bubbles in the liquid from a lower side and a transparent inclined surface that faces obliquely downward and is disposed at a position to which the air bubbles present inside the liquid move up. The transparent inclined surface includes a hydrophilic membrane. The hydrophilic membrane has a contact angle with water of 20 degrees or less. This structural arrangement allows for reducing an attachment of the air bubbles on the transparent inclined surface even when the air bubbles become small. This allows for reducing stay of the air bubbles on the transparent inclined surface and allows for accurately measuring the states of the air bubbles (that is, the size and quantity of the air bubbles).

Подробнее
Номер записи: 81
10-01-2019 дата публикации

FLOW CELL FOR ANALYZING PARTICLES IN A LIQUID TO BE EXAMINED

Номер: US20190011350A1
Автор: Hayden Oliver, Richter Lukas, Ugele Matthias
Принадлежит:

The invention relates to a device for examining particles in a liquid to be examined, comprising a flow passage through which the liquid to be examined is moved. The flow passage has at least one inlet through which at least one sheath fluid flows into the flow passage such that the at least one sheath fluid forms at least one sheath flow in the flow passage. The device further comprises a wave generating device for piezoacoustically generating sound waves which propagate through the flow passage transversely to the flow direction of the liquid to be examined and form wave nodes on a monitoring plane such that particles to be examined of the liquid to be examined are moved onto the monitoring plane and accumulate thereon on the basis of the pressure effect of the sound waves in the transversal direction. 1. An apparatus for examining particles in a liquid to be examined , having a process stage for separating and positioning the particles to be examined , comprising:a flow passage through which the liquid to be examined is moved at a first flow rate, comprising at least one inlet, through which at least one sheath liquid flows into the flow passage at at least one second flow rate such that the at least one sheath liquid forms at least one sheath flow in the flow passage and the liquid to be examined flows through the flow passage longitudinally in a flow direction in relation to the at least one sheath flow,a wave-producing device for piezo-acoustic production of sound waves that propagate through the flow passage transversely to the flow direction of the liquid to be examined and that form wave nodes in an observation plane such that, on account of the effect of pressure of the sound waves in the transversal direction, particles to be examined of the liquid to be examined are displaced transversely into the observation plane and are accumulated there,wherein a value of the at least one second flow rate is chosen in such a way that the at least one sheath flow has ...

Подробнее
Номер записи: 82
14-01-2021 дата публикации

SYSTEMS AND METHODS FOR EVALUATING IMMUNE RESPONSE TO INFECTION

Номер: US20210010924A1
Автор: CAREAGA Diana, MAGARI Robert T., SHIN Sanghyuk, TEJIDOR Liliana
Принадлежит:

Systems and methods for characterizing immune response to infection using cellular analysis, such as a hematological cellular analyzer. In some instances, the immune response may be characterized as normal or abnormal based on one or more blood cell population parameters. In some instances, abnormal characterization may be used to identify patients with sepsis or at elevated risk of developing sepsis.

Подробнее
Номер записи: 83
14-01-2021 дата публикации

FLOW CYTOMETER AND PARTICLE DETECTION METHOD

Номер: US20210010925A1
Автор: HAYASHI Tomoya, MATSUMOTO Shohei, Yamada Kazuhiro
Принадлежит: SYSMEX CORPORATION

A flow cytometer, in which detection of light generated from a particle is less likely to be affected by change in a flow velocity of a liquid flowing in a flow cell, is provided. The flow cytometer includes: a flow cell () in which a liquid flows; a liquid sending unit () configured to send the liquid into the flow cell (); a controller () configured to obtain information related to a flow velocity of the liquid flowing in the flow cell (); a light source () configured to irradiate the liquid flowing in the flow cell () with light; and a detector () configured to detect light generated from a particle in the liquid irradiated with light. The controller () changes a liquid sending condition for the liquid sending unit (), based on the obtained information related to the flow velocity. 1. A flow cytometer comprising:a flow cell in which a liquid flows;a liquid sending unit configured to send the liquid into the flow cell;a controller configured to obtain information related to a flow velocity of the liquid flowing in the flow cell;a light source configured to irradiate the liquid flowing in the flow cell with light; anda detector configured to detect light generated from a particle in the liquid irradiated with light, whereinthe controller changes a liquid sending condition for the liquid sending unit, based on the obtained information related to the flow velocity.2. The flow cytometer according to claim 1 , wherein the controller further changes a detection condition for the detector claim 1 , based on the obtained information related to the flow velocity.3. The flow cytometer according to claim 2 , wherein the controller selectively changes the liquid sending condition for the liquid sending unit or the detection condition for the detector claim 2 , based on the obtained information related to the flow velocity.4. The flow cytometer according to claim 2 , whereinthe controllerchanges the liquid sending condition for the liquid sending unit when a difference between ...

Подробнее
Номер записи: 84
14-01-2021 дата публикации

SYSTEMS AND METHODS FOR EVALUATING IMMUNE RESPONSE TO INFECTION

Номер: US20210011005A1
Автор: CAREAGA Diana, HASAN Mohamad, MAGARI Robert T., SHIN Sanghyuk, TEJIDOR Liliana
Принадлежит:

Systems and methods for characterizing immune response to infection using cellular analysis, such as a hematological cellular analyzer. In some instances, the immune response may be characterized as normal or abnormal based on one or more blood cell population parameters. In some instances, abnormal characterization may be used to identify patients with sepsis or at elevated risk of developing sepsis. 1. A method for characterizing inflammatory response to infection , the method comprising:a. flowing a body fluid sample through a flowcell, the body fluid sample comprising a heterogenous population of circulating cells;b. detecting whether a differentially expressed sepsis cell population parameter is present in the heterogenous population of circulating cells by analyzing one or more cell population parameters selected from the group consisting of MO_DC_SD, NE_DC_SD, NE_DC_MEAN, NNRBC_UMALS_SD, MO_ALL_SD, NE_NO, LY_PC, NNRBC_MALS_SD, WNOP, MO_DC_MEAN, WDOP, NNRBC_UMALS_MEAN, BA_PC, NNRBC_MALS_MEAN, BA_PC, NNRBC_MALS_MEAN, EGC_LMALS_MEAN, NNRBC_DC_SD, NNRBC_LMALS_SD, NNRBC_ALL_MEAN, and combinations thereof based on light scatter and/or direct current impedance measurements of the body fluid sample; andc. characterizing an inflammatory response to infection as abnormal based at least in part on detecting the differentially expressed sepsis cell population parameter.2. The method of wherein the body fluid sample is whole blood.3. The method of wherein the one or more cell population parameters is analyzed for cells from the plurality of cells classified as NNRBC.4. The method of claim 1 , wherein each analyzed cell population parameter is compared to a corresponding reference range.5. The method of wherein the inflammatory response to infection is characterized as abnormal if at least one of the analyzed cell population parameters is outside its corresponding reference range.6. The method of claim 4 , wherein the inflammatory response to infection is characterized as ...

Подробнее
Номер записи: 85
09-01-2020 дата публикации

HIGHLY INTEGRATED OPTICAL PARTICLE COUNTER (OPC)

Номер: US20200011779A1
Автор: Cooper Taylor, Lavrovsky Vladislav Igorevich, MacDonald Aaron Joseph
Принадлежит:

An apparatus and system for detecting and measuring particles entrained in an air stream. The apparatus and system include an enclosure configured to define an aerosol sampling path and an optical path. The aerosol sampling path allows an air stream having entrained particles to pass therethrough. The aerosol sampling path intersects with the optical path. The intersection defines a sensing region. The sensing region may use a band pass filter to improve signal to noise ratio. At least one flow rate sensor may be located near the sensing region. A light source provides a light beam along the optical path. The light beam intersects with the air stream in the sensing region, wherein the light beam may be scattered by entrained particles contained in the aerosol sampling path. 1. An apparatus for detecting and measuring particles entrained in an air stream , the apparatus comprising:an enclosure configured to define an aerosol sampling path and an optical path, the aerosol sampling path allowing an air stream having entrained particles to pass therethrough, wherein the aerosol sampling path intersects with the optical path, the intersection defining a sensing region;at least one flow rate sensor, the at least one flow rate sensor located near the sensing region;a light source providing a light beam along the optical path;an optical particle detection assembly located in the sensing region; andan electronic control assembly,wherein the light beam intersects with the air stream in the sensing region, wherein the light beam may be scattered by entrained particles contained in the aerosol sampling path.2. The apparatus of claim 1 , further comprising an accelerometer assembly located within the enclosure claim 1 , the accelerometer assembly configured to determine the orientation and acceleration of the apparatus.3. The apparatus of claim 1 , further comprising a fan assembly configured to pull air through the aerosol sampling path creating the air stream claim 1 , the fan ...

Подробнее
Номер записи: 86
09-01-2020 дата публикации

PARTICLE ANALYZING APPARATUS, PARTICLE SEPARATING DEVICE, PARTICLE ANALYSIS METHOD, AND PARTICLE SEPARATING METHOD

Номер: US20200011781A1
Автор: KAWANO Makoto
Принадлежит:

A particle analyzing apparatus () includes a processor () and storage (). The processor () acquires a volume magnetic susceptibility of an analyte particle (p). The storage () stores reference data (). The reference data () indicates a volume magnetic susceptibility of a reference particle of the same type as a type of the analyte particle (p) for each of possible crystal forms of the analyte particle (p). The processor () determines a crystal form of the analyte particle (p) on the basis of the volume magnetic susceptibility of the analyte particle (p) and the reference data (). 2. The particle analyzing apparatus according to claim 1 , whereinthe reference data indicates a relationship between a particle diameter and the volume magnetic susceptibility of the reference particle for each of the possible crystal forms of the analyte particle, andthe processor acquires a particle diameter and the volume magnetic susceptibility of the analyte particle, and determines the crystal form of the analyte particle on the basis of the particle diameter and the volume magnetic susceptibility of the analyte particle and the reference data.4. The particle separating device according to claim 3 , whereinthere are a plurality of the particle trapping sections,the plurality of particle trapping sections are arranged in a row extending in the direction in which the liquid medium flows, andthe plurality of particle trapping sections generate magnetic fields each having a different value of a product of the magnetic flux density and the gradient of the magnetic flux density.8. The particle separating method according to claim 7 , further comprising:preparing the liquid medium having a volume magnetic susceptibility that allows the particle having the particular crystal form to be trapped. The present invention relates to particle analyzing apparatuses, particle separating devices, particle analysis methods, and particle separating methods.The present inventors have in the past proposed ...

Подробнее
Номер записи: 87
09-01-2020 дата публикации

CLEANING-PERFORMANCE EVALUATION SYSTEM

Номер: US20200011791A1
Автор: Guo Zhiqiang, Hutamura Haruka, Kawasaki Koji, Kitano Tsukasa, Yazaki Yukihiro
Принадлежит:

The present invention provides a cleaning-performance evaluation system that can acquire evaluation results in real time as quantitative values without such factors as the experience of evaluators causing variations in evaluations. 1. A cleaning-performance evaluation system used for evaluating a cleaning operation inside a work chamber , the system functioning as an inspection system for inspecting the cleaning-performance of a mock contaminant generated inside the work chamber , comprising:a contamination means for administering the mock contaminant to the inside of the work chamber;a waste liquid recovery means for performing the cleaning operation and recovering a cleaning waste-liquid that contains the mock contaminant;a mist generation means for generating a mist of the cleaning waste-liquid;a collection means for collecting inspection air that includes the generated mist; anda detection means for detecting the amount of the mock contaminant included in the inspection air collected by the collection means, whereinthe cleaning operation is evaluated by confirming that the amount of the mock contaminant detected by the detection means is at or below a prescribed value.2. The cleaning-performance evaluation system according to claim 1 , wherein the detection means includes a fine particle detecting part and a fluorescence detecting part claim 1 , the fine particle detecting part detects the total particle concentration of the mock contaminant in the inspection air claim 1 , andthe fluorescence detecting part detects the positive particle concentration in the inspection air, wherebythe positive particle ratio is calculated by the total particle concentration and the positive particle concentration to confirm the amount of the mock contaminant.3. The cleaning-performance evaluation system according to claim 2 , wherein the fluorescence detecting part detects the mock contaminant in the inspection air using laser induced fluorometry.4. The cleaning-performance ...

Подробнее
Номер записи: 88
03-02-2022 дата публикации

MULTIPLEXED ON-CHIP IMPEDANCE CYTOMETRY SYSTEM AND METHOD

Номер: US20220034780A1
Автор: FARMEHINI Vahid, HONRADO Carlos, Liu Yi, MCGRATH John, Swami Nathan, VARHUE Walter
Принадлежит:

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques. 1. A method of operating a microfluidic chip , the method comprising:flowing a sample comprising biologic or particle components in a plurality of microfluidic channels of the microfluidic chip, including an upstream microfluidic channel and a plurality of downstream microfluidic channels, wherein the upstream microfluidic channel is connected to the plurality of downstream microfluidic channels;applying a first set of electric field across a first set of one or more electrodes located in the upstream microfluidic channel to selectively urge the biologic or particle components into one or more lanes of a plurality of lanes in a flow to continuously separate the biologic or particle components to channel inlets of the plurality of downstream microfluidic channels;applying a second set of electric field across a second set of one or more electrodes located in at least one downstream microfluidic channel of the plurality of downstream microfluidic channels, including in a first downstream microfluidic channel; andinterrogating, via the second set of one or more electrodes, electrical responses of the separated biologic or particle components in the at least one downstream microfluidic channel, including electrical responses of the separated biologic or particle components in the first downstream microfluidic channel,wherein the electrical responses are analyzed ...

Подробнее
Номер записи: 89
03-02-2022 дата публикации

IMPEDANCE FLOW CYTOMETRY METHODS

Номер: US20220034781A1
Автор: INGLIS Timothy John Jay, Morgan Hywel, SPENCER Daniel
Принадлежит:

A method of antimicrobial susceptibility testing comprises: preparing samples of microorganisms suspended in an electrolyte, comprising a first sample of the microorganisms unexposed to antimicrobial agents and a second sample of the microorganisms exposed to an antimicrobial agent; passing the first sample through an impedance flow cytometer to obtain a first impedance signal representing one or more components of impedance values of the unexposed microorganisms; passing the second sample through the impedance flow cytometer to obtain a second impedance signal representing one or more components of the impedance values of the exposed microorganisms; comparing the first impedance signal and the second impedance signal; and determining a susceptibility of the microorganisms to the antimicrobial agent based on any differences between the first impedance signal and second impedance signal. A method of impedance flow cytometry comprises: flowing a sample of fluid comprising particles suspended in an electrolyte along a flow channel; applying electrical signals to current paths through the fluid, the current paths comprising at least a first current path, a second current path, a further first current path and a further second current path, wherein the electrical signals applied to the first current path and the further first current path have a frequency, magnitude and phase and the electrical signals applied to the second current path and the further second current path have substantially equal frequency and magnitude and opposite phase to the electrical signals applied to the first current path and the second current path; detecting current flow in the current paths; producing a first summed signal representing the sum of the current flow detected in the first current path and the second current path, and a second summed signal representing the sum of the current flow detected in the further first current path and the further second current path; and obtaining a ...

Подробнее
Номер записи: 90
03-02-2022 дата публикации

MICROPARTICLE MEASURING APPARATUS AND MICROPARTICLE MEASURING METHOD

Номер: US20220034784A1
Автор: Kato Yasunobu
Принадлежит:

To provide a technology of maintaining light detection accuracy at a high level irrespective of individual variations in flow rate of microparticles flowing through a flow channel. The present technology provides a microparticle measuring apparatus including: a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; and a detection timing control section configured to control a detection timing of each light detection section, on the basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section. 1. A microparticle measuring apparatus comprising:a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; anda detection timing control section configured to control a detection timing of each light detection section, on a basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section.2. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls the detection timing of each light detection section in real time.3. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls a detection process period of the second light detection section.4. The microparticle measuring ...

Подробнее
Номер записи: 91
03-02-2022 дата публикации

CELL SORTING DEVICE AND METHOD

Номер: US20220034785A1
Автор: Gu Yi, Lo Yu-Hwa
Принадлежит:

A cell sorting system is provided to comprise: an imaging device including abeam scanner scanning abeam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction; a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, and a cell sorting device in communication with the imaging device and the data processing and control device. 1. A cell sorting system , comprising:an imaging device including a beam scanner scanning a beam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction;a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, wherein the control command is produced during the cell flowing in the channel and is indicative of a sorting decision determined based on one or more cellular attributes ascertained from the cell image data; anda cell sorting device in communication with the imaging device and the data processing and control device, the cell sorting device including two ...

Подробнее
Номер записи: 92
03-02-2022 дата публикации

PARTICLE SEPARATING AND MEASURING DEVICE, AND PARTICLE SEPARATING AND MEASURING APPARATUS

Номер: US20220034864A1
Автор: YONETA Masashi
Принадлежит: KYOCERA CORPORATION

A particle separating and measuring device of the present disclosure includes: a first flow path device including a post-separation flow outlet through which a first fluid containing specific particles to be separated flows out; and a second flow path device on which the first flow path device is placed and including a first flow inlet through which the first fluid flows in, the first flow path device in which the post-separation flow outlet is arranged in a lower surface is placed on the second flow path device in which the first flow inlet is arranged in an upper surface of a first region, the post-separation flow outlet and the first flow inlet are connected so as to face each other, and a size of an opening of the first flow inlet is larger than a size of an opening of the post-separation flow outlet. 1. A particle separating and measuring device comprising:a first flow path device having a plate-like shape and including a pre-separation flow inlet through which a fluid flows in that contains specific particles to be separated, a main flow path connected to the pre-separation flow inlet, a plurality of branch flow paths each connected to the main flow path, and a post-separation flow outlet through which a first fluid flows out that contains the specific particles that have been separated; anda second flow path device having a plate-like shape and having a first region on which the first flow path device is placed, and a second region that serves as a measurement region for the specific particles, the second flow path device including a first flow inlet through which the first fluid flows in, a second flow inlet through which a second fluid not containing the specific particles flows in, a first flow path connected to the first flow inlet and through which the first fluid passes, and a second flow path connected to the second flow inlet and through which the second fluid passes, wherein the first flow path and the second flow path are arranged in the second ...

Подробнее
Номер записи: 93
19-01-2017 дата публикации

METHOD FOR DETERMINING DESIRED CELL TYPE USING EXPRESSION OF miRNA AS INDICATOR

Номер: US20170016077A1
Автор: Endo Kei, Saito Hirohide
Принадлежит:

A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: 1. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells , using the expression of miRNA as an indicator , wherein the method comprises the following steps:(1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and(2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.2. The method according to claim 1 , wherein the step (1) is a step of simultaneously introducing the target sequence of miRNA used as an indicator and two or more mRNAs comprising different marker genes into cells.3. The method according to or claim 1 , wherein the desired cell type is a cell type in which the expression level of miRNA used as an indicator is low claim 1 , and the step (2) is a step of distinguishing a cell type in which the translation level of the marker gene is high.4. The method according to any one of to claims 1 , wherein the desired cell type is a cell claims 1 , type in which the expression level of miRNA used as an indicator is high claims 1 , and the step (2) is a step of distinguishing a cell type in which the translation level of the marker gene is low.5. The method according to any one of to claims 1 , wherein claims 1 , the target sequence of the miRNA is linked to the 5′-terminal side of the marker gene.6. The method according to any one of to claims 1 , wherein the determination is carried out using a flow cytometer.7. The method according to any one of to claims 1 , wherein the determination claims 1 , is carried out using an image analyzer.8. The method according to any one of to claims 1 , which further ...

Подробнее
Номер записи: 94
19-01-2017 дата публикации

SYSTEM AND METHOD FOR ADJUSTING CYTOMETER MEASUREMENTS

Номер: US20170016828A1
Автор: XU Heng, Yan Ming
Принадлежит:

Methods and systems for operating a flow cytometer can include forward scatter values, side scatter values, and fluorescence intensity values for events of an unstained sample and associating the fluorescence intensity values with forward scatter-side scatter side scatter plot regions. Methods and systems for operating a flow cytometer can also include measuring forward scatter values, side scatter values, and fluorescence intensity values for events of a stained sample, determining forward scatter-side scatter plot locations for the events of the stained sample, and for each event of the stained sample, subtracting the fluorescence intensity value associated with the forward scatter-side scatter plot region that contains the forward scatter-side scatter plot location of the stained sample event from the measured fluorescence intensity value of the stained sample event at that forward scatter-side scatter plot location. 1. A method for operating a flow cytometer having a forward scatter detector , a side scatter detector , and a plurality of fluorescent emission detectors , each fluorescent emission detector corresponding to a fluorescence channel , comprising:measuring one or more forward scatter values at the forward scatter detector, one or more side scatter values at the side scatter detector, and one or more fluorescence intensity values at one or more of the plurality of fluorescent emission detectors for one or more events of an unstained sample using the flow cytometer;associating one or more fluorescence intensity values for one of more of the plurality of fluorescent emission detectors with one or more forward scatter-side scatter plot regions based at least in part on the measuring the one or more events of the unstained sample;measuring one or more forward scatter values, one or more side scatter values, and one or more fluorescence intensity values at one or more of the plurality of fluorescent emission detectors for one or more events of a stained ...

Подробнее
Номер записи: 95
21-01-2016 дата публикации

White Blood Cell Analysis System and Method

Номер: US20160018310A1
Автор: Vacca Giacomo, Wu Jiong
Принадлежит:

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

Подробнее
Номер записи: 96
21-01-2016 дата публикации

Basophil Analysis System and Method

Номер: US20160018311A1
Автор: Buhl Michael R., Vacca Giacomo, Wu Jiong
Принадлежит:

Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

Подробнее
Номер записи: 97
21-01-2016 дата публикации

Nucleated Red Blood Cell Analysis System and Method

Номер: US20160018312A1
Автор: Buhl Michael R., Coleman Marilou, Lin Emily H., Vacca Giacomo, Wu Jiong
Принадлежит:

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs. 1. A hematology analyzer for conducting a nucleated red blood cell (nRBC) analysis on a blood sample that contains a plurality of nRBCs , the analyzer comprising:an excitation source positioned to excite particles within the blood sample;a plurality of detectors including (1) an axial light loss detector positioned to measure axial light loss from the excited blood sample, (2) an intermediate angle scatter detector positioned to measure intermediate angle scatter from the excited blood sample, (3) a side scatter detector positioned to measure 90° side scatter from the excited blood sample, and (4) a fluorescence detector positioned to measure fluorescence emitted from the excited blood sample; and (a) dilute the blood sample with a reagent that includes a red blood cell (RBC) lysing agent and a cell membrane permeable, nucleic acid binding fluorescent dye;', '(b) incubate the diluted blood sample of step (a) for an incubation period of time', '(c) deliver the incubated sample from step (b) to a flow cell in the hematology analyzer;', '(d) excite the incubated ...

Подробнее
Номер записи: 98
21-01-2016 дата публикации

Method for Hematology Analysis

Номер: US20160018313A1
Автор: Vacca Giacomo, Wu Jiong
Принадлежит:

A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

Подробнее
Номер записи: 99
21-01-2016 дата публикации

Translocation of a polymer through a nanopore

Номер: US20160018384A1
Автор: Peiming Zhang, Stuart Lindsay
Принадлежит: Arizona Board of Regents of ASU

Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with said first and second volumes of fluid, and means for applying an electric potential difference between said first and second volumes of fluid. In some such embodiments, a conjugate product is provided which comprises charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving a product in the first volume of fluid, the product is directed into the orifice.

Подробнее
Номер записи: 100