METHOD FOR ANALYZING BIOLOGICAL SAMPLE

In this method for analyzing a biological sample, exosomes to which first and second beads are bound are collected from a buffer solution containing the exosomes to which the first and second beads are bound. To the surface of the first beads, a first antibody specifically binding to a first antigen associated with a first disease is fixed. To the surface of the second beads, a second antibody specifically binding to a second antigen associated with a second disease is fixed. The first beads are unbound from the exosomes and then the exosomes to which the second beads are bound are collected. The exosomes to which the second beads are bound are lysed and the second beads and the contents of the exosomes are collected (S19 and S20). The contents of the exosomes are analyzed (S21) and the second beads are counted (S22).

METHOD FOR FABRICATING ANALYSIS SUBSTRATE, ANALYSIS SUBSTRATE, AND ANALYSIS UNIT

A method for fabricating an analysis substrate comprises immobilizing, on an analysis substrate (10), antibodies (41) for reacting specifically with a specific antigen (52) possessed by a substance (50) to be detected. The analysis substrate (10) is immersion-treated with a predetermined treatment liquid, and antibody aggregates (42) in which the antibodies (41) are aggregated are formed in boundary regions between recesses and projections on the analysis substrate (10). The antigen (52) and the antibodies (41) are reacted, and the substance (50) to be detected is captured on the analysis substrate (10) by the antibody aggregates (42).

CONJUGATE FORMATION METHOD, SUBSTANCE OF INTEREST MEASUREMENT METHOD, AND KIT

A method for forming a conjugate of a substance of interest and a magnetic particle to which a specific binding substance that has an ability to specifically bind to the substance of interest has been bound, the method including a step (A1) of reacting the substance of interest and the magnetic particles in a reaction solution, in which the step (A 1 ) includes increasing momentum of the magnetic particles in the reaction solution by changing the magnetic field that is exerted on the magnetic particles.

METHOD FOR ANALYZING BIOLOGICAL SAMPLE

An exosome to be analyzed having a first bead and a second bead bound thereto is collected from a buffer fluid containing the exosome to be analyzed having the first bead and the second bead bound thereto. A first antibody that specifically binds to a first antigen associated with a first disease is fixed to a surface of the first bead. A second antibody that specifically binds to a second antigen associated with a second disease is fixed to a surface of the second bead. The first bead is separated from the exosome, and the exosome having the second bead bound thereto is collected. The exosome having the second bead bound thereto is dissolved, and the second bead and an inclusion of the exosome are collected (S19 and S20). The inclusion of the exosome is analyzed (S21), and the number of second beads is counted (S22).

METHOD FOR ANALYZING BIOLOGICAL SAMPLE

According to the present invention, a second buffer solution is generated by injecting, into a reaction container having injected therein a first buffer solution which contains first beads having fixed to the surfaces thereof a first antibody that specifically binds to a first antigen, a plurality of second beads having fixed to the surfaces thereof a second antibody that specifically binds to an arbitrarily-defined second antigen, and a biological sample containing exosomes having the first and second antigens on the surfaces thereof (S1-S3). The exosomes having the first and second beads bound thereto are recovered from the second buffer solution (S4). The exosomes having the first and second beads bound thereto are separated into the first beads, the second beads, and the exosomes, and the second beads and the exosomes are each recovered (S5 and S6). Matter contained in the exosomes is analyzed (S7), and the number of second beads is counted (S8).

ANALYSIS DEVICE AND ANALYSIS METHOD

An analysis device (1) is provided with a turntable (2), an optical pickup (20), and a control unit (9). The turntable (2) holds a sample analysis disk (70) having a reaction region (66) in which fine particles (64) that bind to a substance (63) to be detected are captured. The optical pickup (20) irradiates the reaction region (66) with laser light (20a), receives the resultant reflected light, and generates a light reception level signal (JS). The control unit (9) continuously generates a plurality of measurement gate signals (GS) for measuring the number of fine particles captured in the reaction region (66), measures the number of fine particles (64) for each of the measurement gate signals (GS) on the basis of the light reception level signal (JS), identifies a measurement gate section (Tiwa) in the reaction region (66) on the basis of the measurement result for each of the measurement gate signals (GS), and adds the number of the fine particles (64) in the measurement gate section ...

ANALYSIS METHOD AND ANALYSIS DEVICE

Laser light (50a) is irradiated on an analysis substrate (1), reflected light from a reaction region (10) is received, and a light reception level signal (JS) is generated. Furthermore, in the reaction region (10), a light reception level signal (JS) having a signal level higher than a prescribed signal level (Lth) is extracted as a particle detection signal (KS), and a substance to be detected (11) is detected on the basis of the extracted particle detection signal (KS). The analysis substrate (1) has: the reaction region (10) on which are captured first particles (20) provided with the substance to be detected (11) and antibodies (21) that recognize the substance to be detected (11), and second particles (30) provided with an antigen (31) that bonds with the antibodies (21), the second particles (30) being formed from a metal; and a non-reaction region (9) on which the reaction region (10) is not formed. The analysis substrate (1) is formed of a resin material.

Analysis Device and Analysis Method

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other.

DISPENSING UNIT

This dispensing unit (1) comprises a disk (100), a cartridge (200), and a holder (300) for dispensing. The disk (100) has a disk shape and has a track area (105) in which recesses (104) and protrusions (103) are arranged alternatingly. The cartridge (200) has a through hole (201), and a well (10) is formed by the through hole (201) and the track area (105) in a state where the cartridge is attached to the disk (100). The holder (300) for dispensing has a holding part (310) that is inserted into the through hole (201) and a guide hole (320) that passes through the holding part (310). The guide hole (320) has a truncated cone shape such that the diameter (R320a) of the opening on the side of the holding unit (310) is smaller than the diameter (R320b) of the opening on the reverse side from the holding unit (310). The center line (CL320) of the guide hole (320) is positioned on a line (CL110) that passes through the center (C100) of the disk (100) and the center (C10) of the well (10).

SAMPLE DETECTION DEVICE AND SAMPLE DETECTION METHOD

A sample detection device 50 is provided with a unit retention part 60, a magnetic field generating part 70, and a drive part 80. The unit retention part 60 retains a specimen analysis container 10 for capturing magnetic fine particles bonded to a detection object substance and storing a solution (extraction liquid 105) for extracting an included substance from the detection object substance. The magnetic field generating part 70 has a magnet 72 for capturing the magnetic fine particles in the specimen analysis container 10. The drive part 80 drives the magnetic field generating part 70 so as to cause the magnet 72 to approach the specimen analysis container 10 so that magnetic fine particles released from bonding with the detection object substance by the solution stored in the specimen analysis container 10 are captured in the specimen analysis container 10 by the magnetic force of the magnet 72.

MAGNET STRUCTURE, CLAMP MECHANISM, AND OPTICAL DISC DEVICE

A magnet structure (1) is provided with a first magnet unit (10) and a second magnet unit (20). The first magnet unit (10) is formed with a first magnet fixing portion (12) which includes a first surface (12a) and a first magnet (14) having a first polarity on the first surface (12a) side. The second magnet unit (20) is formed with a second magnet fixing portion (22) which includes a second surface (22a) and a second magnet (26) having a second polarity, which is the opposite polarity to the first polarity, on the second surface (22a) side. The first surface (12a) and the second surface (22a) are arranged adjacent to each other on the same plane, and form a magnetic attachment surface (1b) that magnetically attaches to a magnetically attached member.

ANALYSIS METHOD AND ANALYZING DEVICE

This analysis method includes the following steps. (Step S2) Radiate laser light onto an analysis substrate (1) which is formed using a resin material and which includes a reaction zone (10) in which a substance to be detected (11) and fine particles (20) comprising a metallic compound for marking the substance to be detected (11) are captured. (Step S4) Extract, as a substrate signal level (DL), a signal level generated upon receipt of reflected light from the analysis substrate (1). (Step S6) Receive reflected light from the reaction zone (10) and generate a received light level signal (JS). (Step S7) Extract, as a fine particle detection signal (KS), the portion of the received light level signal (JS) in the reaction zone (10) that has a signal level higher than the received light level of the reflected light from the analysis substrate (1). (Step S8) Detect the fine particles (20) on the basis of the extracted fine particle detection signal (KS).

ANALYSIS DEVICE AND ANALYSIS METHOD

An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively.

METHOD FOR ANALYZING BIOLOGICAL SAMPLE

A first buffer fluid containing a first bead having fixed to a surface thereof a first antibody that specifically binds to a first antigen is injected into a reaction container, and then a plurality of a second bead having fixed to a surface thereof a second antibody that specifically binds to an optional second antigen, and a biological sample containing an exosome to be analyzed having the first antigen and the second antigen on a surface of the exosome are injected into the reaction container, thereby generating a second buffer fluid (S1 to S3). An exosome having the first bead and the second bead bound thereto is collected from the second buffer fluid (S4). The exosome having the first bead and the second bead bound thereto is separated into the first bead, the second bead, and the exosome, and the second bead and the exosome are individually collected (S5 and S6). An inclusion of the exosome is analyzed (S7), and the number of second beads is counted (S8).

Analysis Device and Analysis Method

An analysis device includes an optical scanning unit and a pulse waveform analysis unit. The optical scanning unit optically scans a surface of a substrate to which analytes binding to particles are fixed, and obtains a detection signal from the substrate. The pulse waveform analysis unit includes an amplitude determination unit configured to determine whether a peak value of the pulse waveform is within a first range smaller than a first level and greater than a second level having a polarity identical to the first level; and a pulse width determination unit configured to determine whether a pulse width of the pulse waveform is within a second range. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which analytes binding to particles are fixed, and obtain a detection signal from the substrate; anda pulse waveform analysis unit configured to analyze a pulse waveform of the detection signal obtained by the optical scanning unit, whereinthe pulse waveform analysis unit comprises:an amplitude determination unit configured to determine whether a peak value of the pulse waveform is within a first range smaller than a first level and greater than a second level having a polarity identical to the first level; anda pulse width determination unit configured to determine whether a pulse width of the pulse waveform is within a second range.2. The analysis device according to claim 1 , wherein the pulse waveform analysis unit detects the pulse waveform of which the peak value is within the first range and of which the pulse width is within the second range.3. An analysis method comprising:optically scanning a surface of a substrate to which analytes binding to particles are fixed, and obtaining a detection signal from the substrate;determining whether a peak value of a pulse waveform of the detection signal is within a first range smaller than a first level and greater than a second level having a polarity ...

SAMPLE ANALYSIS DEVICE AND CAPTURING METHOD FOR EXOSOMES

A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes. 1. A sample analysis device , comprising:a base portion;a recessed portion which is formed on the base portion and in which an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes; anda protruding portion adjacent to the recessed portion,wherein the recessed portion and the protruding portion are periodically arranged on the base portion,and wherein the protruding portion has a width smaller than an average particle diameter of the exosomes.2. The sample analysis device according to claim 1 , wherein the recessed portion has a width larger than the average particle diameter of the exosomes and is smaller than twice a diameter of beads modifying the exosomes.3. The sample analysis device according to claim 2 , wherein the recessed portion has a width smaller than four times the average particle diameter of the exosomes.4. A sample analysis device claim 2 , comprising:a base portion;a recessed portion which is formed on the base portion and in which an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes; anda protruding portion adjacent to the recessed portion,wherein the recessed portion and the protruding portion are periodically arranged on the base portion,and wherein the protruding portion has a width smaller than a diameter of beads modifying the exosomes.5. The sample analysis device according to claim 4 , wherein the recessed portion has a width larger than an average particle diameter of the exosomes and is smaller than ...

ANALYSIS METHOD AND ANALYSIS DEVICE

An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal. 1. An analysis method comprising:irradiating, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured;extracting, as a substrate signal level, a signal level generated when receiving reflected light from a non-reaction region in the analysis substrate at which the reaction region is not formed;receiving reflected light from the reaction region to generate a light reception level signal;extracting a nanoparticle detection signal having a higher signal level than the substrate signal level from the light reception level signal of the reflected light from the reaction region; anddetecting the nanoparticles in accordance with the extracted nanoparticle detection signal.2. The analysis method according to claim 1 , wherein the nanoparticles have a complex refractive index n−ki claim 1 , where an extinction coefficient k of an imaginary part ki is 0.3 or smaller and a refractive index n of a real part is in a range ...

ANALYSIS DEVICE AND ANALYSIS METHOD

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other. 18-. (canceled)9. An analysis method comprising:optically scanning a surface of a substrate to which analytes, in which particles for labeling the analytes are bound to the analytes, are fixed by irradiating laser light;detecting pulse waves included in a detection signal obtained by scanning the substrate;measuring a pulse width of each detected pulse wave and a pulse interval between two pulse waves which are consecutively detected; andcounting the analytes, determining that two particles are bound to one analyte, and determining that an analyte count is one, when two pulse waves are consecutively detected and each pulse wave has a pulse width less than a first reference value, and when the pulse interval between the two pulse waves is less than a predetermined interval.10. The analysis method according to claim 9 , wherein claim 9 , when each pulse wave of the consecutively detected two pulse waves has a pulse width less than the first reference value and the pulse interval between the two pulse waves is equal to or greater than the predetermined interval claim 9 , not counting the consecutively detected two pulse waves as an analyte.11. The analysis method according to claim 9 , wherein claim 9 , when a first pulse wave having a pulse width less than the first reference value is detected claim 9 , and a subsequent pulse wave of the first pulse wave having a pulse width equal ...

METHOD OF CAPTURING EXOSOMES

A first sample solution including exosomes including first to third detection target substances is mixed with a first buffer solution including first nanoparticles including first binding substances which bind to the first detection target substances. The first detection target substances and the first binding substances are bound together, so as to form first complexes of the exosomes and the first nanoparticles. The first complexes are isolated from a mixed solution of the first sample solution and the first buffer solution. The second detection target substances and the second binding substances are bound together, so as to capture the first complexes on a substrate. The second binding substances are fixed onto the substrate. A second buffer solution including second nanoparticles including third binding substances which bind to the third detection target substances is reacted with the first complexes. 1. A method of capturing exosomes comprising the steps of:mixing a first sample solution including exosomes expressing first detection target substances, second detection target substances, and third detection target substances with a first buffer solution including first nanoparticles fixing first binding substances which bind to the first detection target substances, so as to bind the first detection target substances and the first binding substances together to form first complexes of the exosomes and the first nanoparticles;isolating the first complexes from a mixed solution of the first sample solution and the first buffer solution;binding the second detection target substances of the first complexes and second binding substances which bind to the second detection target substances together, so as to capture the first complexes on a substrate, the second binding substances being fixed onto the substrate; andreacting a second buffer solution including second nanoparticles fixing third binding substances which bind to the third detection target substances with ...

Analysis Method and Analysis Device

An analysis substrate is irradiated with laser light, and reflected light from reaction region is received to generate a light reception level signal. Particle detection signal having a signal level higher than a predetermined signal level is extracted from the light reception level signal in the reaction region, so as to detect detection target substance in accordance with the extracted particle detection signal. The analysis substrate has the reaction region on which the detection target substance, primary particle provided with antibody for labeling the detection target substance, and secondary particle formed of metal and provided with antigen to be bound to the antibody are captured. 1. An analysis method comprising:irradiating, with laser light by an optical pickup, an analysis substrate having a reaction region on which a detection target substance is bound to the reaction region, the detection target substance is bound to a primary particle provided with an antibody to be bound to the detection target substance, the primary particle is bound to a secondary particle, having a complex refractive index different from the primary particle, formed of metal and provided with an antigen to be bound to the antibody;receiving reflected light from the reaction region to generate a light reception level signal by the optical pickup;extracting a particle detection signal having a signal level higher than a predetermined signal level from the light reception level signal in the reaction region by a determination circuit; anddetecting the detection target substance in accordance with the extracted particle detection signal by a counter circuit,wherein the light reception level signal derived from the primary particle bound to the secondary particle is higher than the predetermined signal level.2. The analysis method according to claim 1 , wherein the predetermined signal level is a signal level generated when reflected light is received from a region in which the ...

Magnet Structure, Clamping Mechanism, and Optical Disc Apparatus

A magnet structure includes first and second magnet units. The first magnet unit is provided with a first magnet fixing section that includes a first surface and a first magnet having a first polarity on the first surface's side. The second magnet unit is provided with a second magnet fixing section that includes a second surface and a second magnet having a second polarity on the second surface's side, the second polarity being an opposite polarity to the first polarity. The first and second surfaces are located next to one another on the same plane to form a magnetic attachment surface which is magnetically attached to a magnetically-attached object. The second magnet unit is supported by the first magnet unit so as to move in a predetermined range.

Analysis device and analysis method

An analysis device includes a turntable holding a substrate, an optical pickup driven in a direction perpendicular to a rotation axis of the turntable and configured to emit laser light to reaction regions and to receive reflected light from the respective reaction regions, an optical pickup drive circuit, and a controller. The reaction regions are formed at positions different from the center of the substrate. The center of the substrate is located on the rotation axis of the turntable. The optical pickup detects a reception level of the reflected light to generate a light reception level signal. The controller controls a turntable drive circuit to rotate the substrate, controls the optical pickup drive circuit to drive the optical pickup, and specifies the respective reaction regions in accordance with a positional information signal and the light reception level signal.

Dispensing Unit

The dispensing unit includes a disc, a cartridge, and a dispensing holder. The disc has a disc shape and includes a track region provided with recesses and projections alternately arranged in a radial direction. The cartridge includes a penetration hole, and a well is formed by the penetration hole and the track region in a state in which the cartridge is attached to the disc. The dispensing holder includes a holding part to be inserted into the penetration hole, and a guide hole penetrating the holding part. The guide hole has a truncated cone shape in which a first opening diameter on the holding part side is smaller than a second opening diameter on a side opposite to the holding part, and a center line of the guide hole is located on a line passing through the center of the disc and the center of the well. 1. A dispensing unit comprising:a disc which has a disc shape and includes a track region provided with recesses and projections alternately arranged in a radial direction;a cartridge which includes a penetration hole and in which a well for storing a solution is formed by the penetration hole and the track region in a state in which the cartridge is attached to the disc; anda dispensing holder which includes a holding part having a projection shape and formed corresponding to the well, and a guide hole penetrating the holding part, and is held in the cartridge by inserting the holding part into the well, whereinthe guide hole has a truncated cone shape in which a first opening diameter on the holding part side is smaller than a second opening diameter on a side opposite to the holding part, andthe guide hole is formed such that a center line of the guide hole is located on a line passing through the center of the disc and the center of the well when the guide hole is viewed from the dispensing holder side in a state in which the cartridge is attached to the disc and the dispensing holder is held in the cartridge.2. The dispensing unit according to claim 1 , ...

Analysis device and analysis method

An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively.

Analysis Unit, Washing Device, and Washing Method

An analysis unit for quantitating detection target substances bound to antibodies includes wells and inclination parts. The wells each have a hole-like shape defined by an opening, an inner circumferential surface, and a bottom. The inclination parts each have an inclined surface connected to the inner circumferential surface and inclined downward such that whose height with respect to the bottom decreases as a distance from an outer circumferential side of the well increases.

Analysis Device and Analysis Method

An analysis device includes a turntable, an optical pickup, and a controller. The turntable holds a specimen analysis disc having reaction regions on which nanoparticles binding to substances to be detected are captured. The optical pickup emits laser light to each reaction region, receives a reflected light from each reaction region, and generates a light reception level signal. The controller sequentially generates a plurality of measurement gate signals for counting the number of the nanoparticles captured on each reaction region, counts the number of the nanoparticles of each of the measurement gate signals based on the light reception level signal, specifies a measurement gate section in each reaction region according to a measurement result per measurement gate signal, and adds up the number of the nanoparticles of the respective measurement gate signals in the measurement gate section. 1. An analysis device comprising:a turntable holding a specimen analysis disc having a reaction region on which nanoparticles binding to substances to be detected are captured;a turntable drive unit configured to rotate the turntable;a turntable drive circuit configured to control the turntable drive unit;an optical pickup driven in a direction perpendicular to a rotation axis of the turntable, and configured to emit laser light to the reaction region, to receive a reflected light from the reaction region, and to generate a light reception level signal;an optical pickup drive circuit configured to control an operation of the optical pickup; anda controller configured to control the turntable drive circuit and the optical pickup drive circuit,wherein the controller sequentially generates a plurality of measurement gate signals for counting a number of the nanoparticles captured on the reaction region, counts the number of the nanoparticles of each of the measurement gate signals based on the light reception level signal, specifies a measurement gate section in the reaction ...

BIOLOGICAL SAMPLE ANALYSIS METHOD

A first buffer fluid containing a first bead having fixed to a surface thereof a first antibody that specifically binds to a first antigen is injected into a reaction container, and then a plurality of a second bead having fixed to a surface thereof a second antibody that specifically binds to an optional second antigen, and a biological sample containing an exosome to be analyzed having the first antigen and the second antigen on a surface of the exosome are injected into the reaction container, thereby generating a second buffer fluid. An exosome having the first bead and the second bead bound thereto is collected from the second buffer fluid. The exosome having the first bead and the second bead bound thereto is separated into the first bead, the second bead, and the exosome, and the second bead and the exosome are individually collected. 1. A biological sample analysis method comprising:injecting, into a reaction container, a first buffer fluid containing a plurality of a first bead having fixed to a surface thereof a first antibody that specifically binds to a first antigen associated with a particular disease;injecting, into the reaction container, a plurality of a second bead having fixed to a surface thereof a second antibody that specifically binds to an optional second antigen that is the same as or different from the first antigen, and a biological sample containing an exosome to be analyzed having the first antigen and the second antigen on a surface of the exosome, thereby generating a second buffer fluid;collecting the exosome to be analyzed having the first bead and the second bead bound thereto, from the second buffer fluid;separating the collected exosome to be analyzed having the first bead and the second bead bound thereto, into the first bead, the second bead, and the exosome to be analyzed, and collecting the second bead and the exosome to be analyzed; andanalyzing an inclusion of the collected exosome to be analyzed, and counting the number of ...

Method for forming conjugate, method for measuring object substance, and kit

【課題】対象物質と第１の特的結合物質を結合させた磁性粒子と反応槽に結合された第２の特異的結合物質との反応時間を低減可能な、結合体の形成方法、及び対象物質の測定方法、並びに前記方法に用いられるキットを提供する。【解決手段】対象物質と、前記対象物質に対し特異的結合を行う第１の特異的結合物質を結合させた磁性粒子と、反応槽の内壁に結合された前記対象物質に対し特異的結合を行う第２の特異的結合物質との結合体を形成する方法であって、前記反応槽に収容された、前記対象物質と前記磁性粒子とを含む反応液中で、前記対象物質と前記磁性粒子との結合反応、及び前記対象物質と前記第２の特異的結合物質との結合反応を行う工程（Ａ）を含み、前記工程（Ａ）が、前記磁性粒子に作用する磁場を変化させて、前記反応液中での前記磁性粒子の運動量を増大させることを含む、方法。【選択図】なし

Method for forming conjugate of object substance and magnetic particle, method for measuring object substance, and kit

【課題】対象物質と磁性粒子との反応時間を低減可能な、対象物質と磁性粒子との結合体の形成方法、及び対象物質の測定方法、並びに前記方法に用いられるキットを提供する。【解決手段】対象物質と、前記対象物質に対し特異的結合を行う特異的結合物質を含む磁性粒子との結合体を形成する方法であって、前記対象物質と、前記磁性粒子とを液体中で反応させる工程（Ａ）を含み、前記工程（Ａ）が、前記磁性粒子に作用する磁場を変化させて、前記液体中での前記磁性粒子の運動量を増大させることを含む、方法。【選択図】なし

Method of producing sample

【課題】従来の方法と比較し、検出対象物質を高い再現性で検出可能な試料製造方法を提供する。【解決手段】試料製造方法は、基板１０上に分注された溶液中で、検出対象物質４２に、第１抗体４１と第２抗体４３にビオチン４４を結合させたビオチン化抗体４５とを結合して生成された複合体４０が第１抗体４１を介して固定された基板１０に対して、複合体４０の少なくとも一部が露出するまで乾燥させる乾燥工程を含んでいる。試料製造方法は、乾燥工程の後に、基板１０に固定された複合体４０のビオチン化抗体４５に対してアビジンで修飾された粒子４６を結合させる粒子結合工程を含んでいる。【選択図】図９

Analysis method and analysis device

An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.

Optical disk and method of producing the same

Sample analysis device and capturing method for exosomes

A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes.

Analyzer and analysis method

【課題】トラック間クロストークによる影響を抑制し、従来よりも短い時間で微粒子を計測できる分析装置及び分析方法を提供する。【解決手段】分析装置１は光ピックアップ１０と信号処理回路２０とを備える。信号処理回路２０は光ピックアップ１０が受光した検出光Ｌｂ３，Ｌｂ４から受光レベル信号ＲＳ３，ＲＳ４を生成し、検出光Ｌｂ１，Ｌｂ２から受光レベル信号ＲＳ１，ＲＳ２を生成する。信号処理回路２０は受光レベル信号ＲＳ３，ＲＳ４と受光レベル信号ＲＳ１，ＲＳ２とを加算して検出信号ＤＳを生成し、受光レベル信号ＲＳ３，ＲＳ４から受光レベル信号ＲＳ１，ＲＳ２を減算して差信号ＳＳを生成する。信号処理回路２０は検出信号ＤＳから微粒子検出信号ＢＳを生成し、差信号ＳＳから計測補正信号ＣＳを生成し、これらの排他的論理和が１となる場合に微粒子計測信号ＨＳを生成する。【選択図】図１

Optical element, deflecting element using optical element, and method and device for optical control

(57)【要約】 【課題】 高速かつ、所望の偏向角で光の偏向を行う。 【解決手段】 信号光の光源１２と、信号光の光源とは 波長の異なる制御光の光源１１と、くさび形の光強度分 布調整手段６と、光応答組成物からなる光学素子９とか ら少なくとも構成され、この光学素子中に制御光により 屈折率分布を形成し、この屈折率分布で信号光の偏向を 制御する。

Optical element, optical control method and device using this optical element, and method of manufacturing optical element

An optical element, optical control device and optical control method are provided which demonstrate a large, rapid optical response using a laser light of low output as a control light. A signal light 1 is emitted from an optical source 1 . A signal light 2 emitted from an optical source 2. A control light and the signal light are converged by a condenser lens 7, and irradiate an optical element 8. Only the signal light is detected by a photodetector 22 through a light receiving lens 9 and a wavelength selection filter 20. A thermal lens is formed reversibly in the optical element and switching the control light ON and OFF modulates the intensity of the signal light. The optical element has a laminar structure comprising a heat transfer film/heat insulation film/light absorption film/heat insulation film/heat transfer film, for example, and a sufficiently large and rapid optical response is obtained using low output laser light as the control light by adjusting the thickness of the light absorption film so that it does not exceed twice the confocal length of the converged control light.

Optical element, optical control method and apparatus using the optical element, and method of manufacturing the optical element

An optical element, an optical control method and an optical control apparatus, wherein use is made of a low-power laser beam as a control beam to yield a light response of sufficient magnitude and speed. A control beam is projected from a light source (1), while a signal beam is from a light source (2). The control beam and the signal beam are converged by a condenser lens (7) and thrown onto an optical element (8). These beams pass through a light receiving lens (9) and a wavelength selection filter (20), and only the signal beam is detected with a photodetector (22). By turning the control beam on and off, a thermal lens is reversibly formed in the optical element, realizing the modulation of the signal beam intensity. The optical element may be formed in a laminated structure comprising, for example, a heat transfer layer, a heat retaining layer, and a heat transfer layer, and the thickness of the light absorbing layer is adjusted so as not to exceed two times the confocal distance of the converged control beam, thereby providing a light response of sufficient magnitude and speed by the use of a low-power laser beam as a control beam.

Light-controlling method and light-controlling device

(57)【要約】 【目的】 充分な大きさおよび速度の光応答を再現性良 く光応答性の光学素子から引き出すような光制御方法お よび光制御装置を提供する。 【構成】 光源１から制御光が、光源２から信号光が射 出する。制御光および信号光は集光レンズ７で収束さ れ、光学素子８に照射される。受光レンズ９および波長 選択透過フィルター２０を経て光検出器２２で信号光の みが検出される。制御光のＯＮ、ＯＦＦにより信号光の 透過率が可逆的に増減し、信号光の強度変調が実現す る。受光レンズの開口数を集光レンズの開口数よりも実 質的に小さく設定することにより、充分な大きさおよび 速度の光応答をナフトキノンまたはアントラキノン誘導 体を含有する光応答性の光学素子から引き出すことが可 能となる。

Method of capturing exosomes

A first sample solution including exosomes including first to third detection target substances is mixed with a first buffer solution including first nanoparticles including first binding substances which bind to the first detection target substances. The first detection target substances and the first binding substances are bound together, so as to form first complexes of the exosomes and the first nanoparticles. The first complexes are isolated from a mixed solution of the first sample solution and the first buffer solution. The second detection target substances and the second binding substances are bound together, so as to capture the first complexes on a substrate. The second binding substances are fixed onto the substrate. A second buffer solution including second nanoparticles including third binding substances which bind to the third detection target substances is reacted with the first complexes.

Analysis device and analysis method

An analysis device includes a turntable, an optical pickup, and a controller. The turntable holds a specimen analysis disc having reaction regions on which nanoparticles binding to substances to be detected are captured. The optical pickup emits laser light to each reaction region, receives a reflected light from each reaction region, and generates a light reception level signal. The controller sequentially generates a plurality of measurement gate signals for counting the number of the nanoparticles captured on each reaction region, counts the number of the nanoparticles of each of the measurement gate signals based on the light reception level signal, specifies a measurement gate section in each reaction region according to a measurement result per measurement gate signal, and adds up the number of the nanoparticles of the respective measurement gate signals in the measurement gate section.

Optical record medium

(57)【要約】 【課題】 電子供与性呈色化合物と電子受容性顕色剤と からなる２成分系サーモクロミック材料をマスク層とし て有する光記録媒体は良好なディスク超解像を示すもの の、反面、耐光性が十分でない。 【解決手段】 電子供与性呈色化合物として、特定の構 造を有するジアザフタリド化合物を使用する。

Method for recovering useful substance from waste plastic

(57)【要約】 【課題】 ポリカーボネート樹脂を主成分とする廃プラ スチックを大量にリサイクル処理するために、ポリカー ボネート樹脂の分解時間を大幅に短くすることができる 廃プラスチックから有用物を回収できる方法を提供す る。 【解決手段】 ポリカーボネート樹脂を主成分とする廃 プラスチックから有用物を回収する有用物回収方法にお いて、前記廃プラスチックと、分解剤として下記の一般 式１で表される第１級アミンとが存在する溶液中にて、 前記廃プラスチック中の前記ポリカーボネート樹脂を化 学的に分解し、分解生成物を有用物として回収する。 Ｒ−ＮＨ ２ … （１） （式中Ｒは炭素数１〜５の直鎖のアルキル基）

光記録媒体及びその再生装置並びに光記録媒体の真贋判定装置

(57)【要約】 【課題】 第三者が記録情報の内容や構成を容易に把握 することができず、ひいては偽造を防止することがで き、また、真贋を判定することができる光記録媒体及び その再生装置を提供する。 【解決手段】 光記録媒体１の基板は、例えば波長７０ ０ｎｍの光の透過率が３０％以下であり、かつ６７０ｎ ｍの光の透過率が１％以下の分光特性を有する。再生装 置は６７０ｎｍの光を照射した場合の透過率が所定値で ない場合には光記録媒体１を「偽」と判断してリジェク トし、所定値の場合には光記録媒体１を「真」と判断し て７００ｎｍの光を照射して読み取った信号を再生す る。

Conjugate formation method, substance of interest measurement method, and kit

A method for forming a conjugate of a substance of interest and a magnetic particle to which a specific binding substance that has an ability to specifically bind to the substance of interest has been bound, the method including (A1) reacting the substance of interest and the magnetic particles in a reaction solution, in which (A1) includes increasing momentum of the magnetic particles in the reaction solution by changing the magnetic field that is exerted on the magnetic particles.

光ディスク

(57)【要約】 【課題】 融解し難いベース材料中にマスク材料を分散 させて流れ難くすることにより、繰り返し使用回数を大 幅に向上させることができる光ディスクを提供する。 【解決手段】 情報を記録再生する層６，１０と、照射 光強度が強くなると光透過率が上がるマスク効果を有す るマスク層４とが積層して形成され、前記マスク層を透 過する光スポット径が前記マスク層に入射する光スポッ ト径よりも実質的に縮小する様になされている光ディス クにおいて、前記マスク層は、前記マスク効果を生じる 温度で融解するマスク材料１２Ａをベース材料１２Ｂ中 に分散させることにより形成される。これにより、繰り 返し使用回数を大幅に向上させることができる。

Optical storage medium

An optical disc has a first transparent substrate, a second substrate, and at least a first and a second recording layer formed between the substrates. The first substrate has a first surface and an opposing second surface, the first surface is a beam incidence surface for a laser beam, the second surface having a first concave section and a first convex section formed adjacent to each other thereon, the first concave section being farther than the first convex section from the incidence surface, first pre-pits being formed on the first concave section. The second substrate has a second concave section and a second convex section formed adjacent to each other thereon, the second concave section being farther than the second convex section from the incidence surface, second pre-pits being formed on the second concave section, a top surface of each second pre-pit being closer than a top surface of the second convex section to the beam surface. The second recording layer is farther than the first recording layer from the incidence surface. The second recording layer is formed with a dye mixture of cyanine dye and imonium dye having relations Wa>Wb and na>nb, in which Wa and Wb are a mass of the cyanine dye and the imonium dye, respectively, and “na” and “nb” are refractive indices of the cyanine dye and the imonium dye, respectively, to the laser beam having a specific wavelength, and having a relation na−nc>0.1 in which “nc” is a refractive index of the dye mixture.

分注ユニット

【課題】溶液をウェルに注入したときに発生する気泡の付着を抑制する分注ユニットを提供する。【解決手段】分注ユニット１はディスク１００とカートリッジ２００と分注用ホルダ３００とを備える。ディスク１００は円板形状を有し、凹部１０４と凸部１０３とが半径方向に交互に配置されたトラック領域１０５を有する。カートリッジ２００は貫通孔２０１を有し、ディスク１００に装着された状態において貫通孔２０１とトラック領域１０５とによりウェル１０が構成される。分注用ホルダ３００は貫通孔２０１に挿入される保持部３１０と保持部３１０を貫通するガイド孔３２０とを有する。ガイド孔３２０は保持部３１０側の第１の開口径Ｒ３２０ａが保持部３１０とは反対側の第２の開口径Ｒ３２０ｂよりも小さい円錐台形状を有し、ガイド孔３２０の中心線ＣＬ３２０がディスク１００の中心Ｃ１００とウェル１０の中心Ｃ１０とを通る線ＣＬ１１０上に位置する。【選択図】図５

検体分析カートリッジ、及びその製造方法

【課題】液漏れ耐久性が高い検体分析カートリッジ等を提供すること。【解決手段】少なくとも片面にマイクロ流路が形成された第１の基材と、前記第１の基材のマイクロ流路が形成されている面に対向して配された第２の基材と、前記第１の基材及び前記第２の基材を接着する光硬化性樹脂層と、を備え、前記第１の基材及び前記第２の基材が、シクロオレフィンポリマー及びシクロオレフィンコポリマーの少なくとも１種を含む、検体分析カートリッジ。【選択図】図１

樹脂成形物表面層の改質方法およびそのための装置および表面層が改質された樹脂成形物、および樹脂成形物表面層の着色方法およびそのための装置および表面層が着色された樹脂成形物、および表面層の改質により機能性を付与された樹脂成形物

(57)【要約】 【課題】 樹脂成形物の表面へ前記樹脂と親和性があ り、かつ昇華性の有機化合物を均一に浸透・分散させ、 樹脂表面層の改質および／または着色を行う。 【解決手段】 樹脂成形物、および、前記樹脂と親和性 があり、かつ昇華性の有機化合物とを密閉式容器に入 れ、内部の圧力および温度を調節して前記有機化合物の 飽和昇華圧状態に置くことによって、前記有機化合物蒸 気が前記樹脂成形物表面に均一に付着し、更に、内部に 浸透・分散していくようにし、樹脂表面層の改質および ／または着色を行うことができる。また、樹脂表面層の 改質により機能性を付与することができる

光ディスクの製造方法

【課題】剥離層の剥離が容易で歩留まりの良好な２層の読み出し層及び記録層を有する光ディスクの製造方法を提供する。 【解決手段】基板１の第１の情報ピット上に剥離層２、半透過層３を順次積層する一方、基板４の第２の情報ピット上に反射層５を形成し、次に、接着層６を反射層５上に塗布して、反射層５側に半透過層３が対向配置するようにして、基板１を基板４上に載置した状態で紫外線を照射して貼り合せ、次に、剥離層２と共に基板１を基板４から剥離して、表面に第１の情報ピットが形成された半透過層３を基板４に転写し、次に、接着層８を半透過層３上に塗布した後、薄い光透過性シート７を基板４に載置した状態で紫外線を照射して貼り合せて光ディスクを作製する際、剥離層２の材料は、半透過層３及び基板１のいずれに対しても密着性が低い材料であり、かつ剥離層２の形成は、真空成膜法を用いる。 【選択図】 図３

分析用ユニット、洗浄装置、及び洗浄方法

【課題】ウェルを洗浄する際に、結合された微粒子の剥がれを低減することが可能な分析用ユニット、洗浄装置、及び洗浄方法を提供する。【解決手段】特定の抗原を有する検出対象物質を定量するための分析用ユニット１００はウェル１３０と傾斜部１４０とを備える。ウェル１３０は開口部Ａ１３０と内周面Ｐ１３０と底面Ｂ１３０とを含む穴形状を有する。傾斜部１４０はウェル１３０の内周面Ｐ１３０に接続し、ウェル１３０の外周側から底面Ｂ１３０に対する高さが低くなるように傾斜している傾斜面を有する。洗浄装置１はステージ２と吐出ノズル１１と制御部３０とを備える。ステージ２には分析用ユニット１００が装着される。吐出ノズル１１はウェル１３０内へ溶液ＣＳを注入する。制御部３０は吐出ノズル１１が傾斜面に接近または離隔するようにステージ２または吐出ノズル１１を制御し、溶液ＣＳを傾斜面へ吐出するように吐出ノズル１１を制御する。【選択図】図１

Biological sample analysis method

An exosome to be analyzed having a first bead and a second bead bound thereto is collected from a buffer fluid containing the exosome to be analyzed having the first bead and the second bead bound thereto. A first antibody that specifically binds to a first antigen associated with a first disease is fixed to a surface of the first bead. A second antibody that specifically binds to a second antigen associated with a second disease is fixed to a surface of the second bead. The first bead is separated from the exosome, and the exosome having the second bead bound thereto is collected. The exosome having the second bead bound thereto is dissolved, and the second bead and an inclusion of the exosome are collected. The inclusion of the exosome is analyzed, and the number of second beads is counted.

Optical storage medium

An optical disc has a first transparent substrate, a second substrate, and at least a first and a second recording layer formed between the substrates. The first substrate has a first surface and an opposing second surface, the first surface is a beam incidence surface for a laser beam, the second surface having a first concave section and a first convex section formed adjacent to each other thereon, the first concave section being farther than the first convex section from the incidence surface, first pre-pits being formed on the first concave section. The second substrate has a second concave section and a second convex section formed adjacent to each other thereon, the second concave section being farther than the second convex section from the incidence surface, second pre-pits being formed on the second concave section, a top surface of each second pre-pit being closer than a top surface of the second convex section to the beam surface. The second recording layer is farther than the first recording layer from the incidence surface. The second recording layer is formed with a dye mixture of cyanine dye and imonium dye having relations Wa>Wb and na>nb, in which Wa and Wb are a mass of the cyanine dye and the imonium dye, respectively, and “na” and “nb” are refractive indices of the cyanine dye and the imonium dye, respectively, to the laser beam having a specific wavelength, and having a relation relation na−nc>0.1 in which “nc” is a refractive index of the dye mixture.

Modification method of surface layer of molded resin article, and modification apparatus of surface layer of molded resin article

A modification method of the surface layer of a molded resin article which comprises the steps of: placing, in a first closed space, an organic compound having sublimation properties and an affinity for a resin of a molded resin article to be coated; on the other hand, placing the molded resin article in a second closed space; controlling a temperature in the second closed space so as to be equal to or higher than the temperature in the first closed space; bringing a pressure in the first closed space to a saturated sublimation pressure state of the organic compound; controlling a pressure in the second closed space so as to be equal to or lower than the pressure in the first closed space; subsequently, connecting the first closed space to the second closed space to form a third closed space in which the first closed space is combined with the second closed space, and further controlling the temperature and the pressure so that the whole of the third closed space may be in the saturated sublimation pressure state of the organic compound; allowing a vapor of the organic compound with which the first closed space before the connection is filled to diffuse into the second closed space before the connection; uniformly depositing the vapor of the organic compound on the surface of the molded resin article; and allowing the deposited organic compound to penetrate/disperse from the surface of the molded resin article into its inside.

Specimen analysis cartridge and manufacturing method for the same

Disclosed is a specimen analysis cartridge that includes: a first base having a micro flow path formed in at least one surface; a second base disposed so as to oppose a surface at which the micro flow path of the first base is formed; and a photocurable resin layer for adhering the first base and the second base to each other, and the first base and the second base each contain at least one of cycloolefin polymer and cycloolefin copolymer.

Method of modifying surface layer of molded resin

A modification method of the surface layer of a molded resin article which comprises the steps of: placing, in a first closed space, an organic compound having sublimation properties and an affinity for a resin of a molded resin article to be coated; on the other hand, placing the molded resin article in a second closed space; controlling a temperature in the second closed space so as to be equal to or higher than the temperature in the first closed space; bringing a pressure in the first closed space to a saturated sublimation pressure state of the organic compound; controlling a pressure in the second closed space so as to be equal to or lower than the pressure in the first closed space; subsequently, connecting the first closed space to the second closed space to form a third closed space in which the first closed space is combined with the second closed space, and further controlling the temperature and the pressure so that the whole of the third closed space may be in the saturated sublimation pressure state of the organic compound; allowing a vapor of the organic compound with which the first closed space before the connection is filled to diffuse into the second closed space before the connection; uniformly depositing the vapor of the organic compound on the surface of the molded resin article; and allowing the deposited organic compound to penetrate/disperse from the surface of the molded resin article into its inside.

Method of modifying surface layer of molded resin

熱可塑性樹脂の回収方法

【課題】光記録媒体の廃棄物等から基板に使用されている熱可塑性樹脂だけを回収することができる回収方法を提供する。 【解決手段】熱可塑性樹脂と、無機金属と、３次元硬化樹脂とを含む一体成形物から熱可塑性樹脂を回収する方法において、熱可塑性樹脂を溶解し、無機金属及び３次元硬化樹脂を溶解しない第一の有機溶剤１６中で、熱可塑性樹脂のみを溶解して固形物を残留させる溶解工程と、第一の有機溶剤中から、残留している固形物を濾過して分離する濾過工程と、第一の有機溶剤と混和し、且つ熱可塑性樹脂を溶解しない有機溶剤であって、第一の有機溶剤より沸点が高い第二の有機溶剤２２中に、熱可塑性樹脂のみを溶解した第一の有機溶剤を混入して熱可塑性樹脂を析出させる析出工程と、第一の有機溶剤と第二の有機溶剤とが混和している溶液から前記第一の有機溶剤を留出させて回収する回収工程とを実行する。 【選択図】 図１

表面層改質方法およびその方法で製造される機能性薄膜

(57)【要約】 【課題】 被覆対象物の表面層組成物をそれと相互作用 を起こす昇華性物質によって改質し、均一な膜厚および 組成の機能性薄膜を得る。 【解決手段】 被覆対象物の表面層組成物１と相互作用 を起こす昇華性物質２を、閉じられた空間内に置き、更 にこの空間内を前記昇華性物質２の飽和昇華圧状態に し、前記昇華性物質２蒸気を前記被覆対象物表面の前記 表面層組成物１に付着させ、付着した前記昇華性物質２ を更に前記表面層組成物１の表面から表面層内部に浸透 ・分散させ、前記表面層組成物１と相互作用させる表面 層改質方法である。

廃プラスチックからのビスフェノールａの回収方法

【課題】 低純度のビスフェノールＡを高純度のビスフェノールＡとして回収できるビスフェノールＡの回収方法を提供する。 【解決手段】 フェノールとアセトンとを酸性触媒の存在下にて反応させてビスフェノールＡを含む反応生成物を得る主反応工程２と、反応生成物を蒸留して気化物を分離する蒸留分離工程４と、気化物が分離された反応生成物をビスフェノールＡとフェノールとの付加物結晶と母液とに分離すると共に、付加物結晶からビスフェノールＡを回収する晶析・回収工程６と、分離された母液を主反応工程へ再循環させる循環工程８と、よりなるビスフェノールＡの製造方法において、廃プラスチックを熱分解または化学的に分解して得られた分解生成物から回収された低純度のビスフェノールＡを含む粗溶液を付加物結晶の分離された母液中、または気化物が除去された反応生成物中に混合させる混合工程１０を有する。 【選択図】 図１

光記録媒体

(57)【要約】 【課題】 微小なピットあるいは溝が形成された基板上 にスピンコート法若しくは加熱真空蒸着法により有機化 合物を含有する薄膜が形成されてなる超解像光ディスク において、溶剤により基板が侵される、ピットあるいは 溝が上記の有機化合物により埋められる、昇華性を示さ ない化合物を使用できないなどの問題がある。 【解決手段】 基板上に、真空容器内で有機化合物を含 有する液体状態の薄膜形成材料を噴霧ノズルから噴霧し た薄膜層を形成する。

光ディスクのリサイクルボックス

(57)【要約】 【課題】 光ディスクの回収、分別が容易になると ともに、廃棄された光ディスクが有している著作権の不 正使用を防ぐことができる。 【解決手段】 光ディスクがちょうど入る大きさに開口 したディスク挿入口１と、このディスク挿入口１より挿 入された光ディスクに記録されている情報を消滅させる ために、少なくとも前記光ディスクの情報記録層に達す る溝状の傷を複数形成する溝形成手段４とを有する光デ ィスクのリサイクルボックス。

光ディスク

【課題】透明性を保持しつつ、耐熱性を向上させたポリ乳酸を用いた光ディスクを提供する。 【解決手段】ポリ乳酸を主成分とする光透過性基板２の凹凸ピット上に反射層３又は記録層、第１保護層４が順次積層され、積層方向と反対側の光透過性基板２に第２保護層５が形成され、第２保護層５側からレーザ光を照射して前記凹凸ピットの情報を再生、又は前記記録層の記録再生を行う光ディスク１において、第１及び第２保護層４、５は、同じ厚さで同種類の紫外線硬化樹脂である。 【選択図】図１

光ディスク

(57)【要約】 【課題】 記録時にも再生時にも十分なマスク効果を発 揮することができる光ディスクを提供する。 【解決手段】 情報を記録再生する層６と、照射光強度 が強くなると光透過率が上がるマスク効果を有するマス ク層１２とが積層して形成され、前記マスク層を透過す る光スポット径が前記マスク層に入射する光スポット径 よりも実質的に縮小して前記情報を記録再生する層に照 射される光ディスクであって、前記マスク層は、感度の 低い低感度マスク膜１２Ｂと、感度の高い高感度マスク 膜１２Ａと、これらの２つのマスク膜間に介在されて前 記２つのマスク膜の材料の混合を防止する隔離膜１４と よりなる。これにより、記録時にも再生時にも十分なマ スク効果を発揮することができる。

エクソソームの検出方法

【課題】従来よりも疾患特異性を高め、診断の精度や確度を向上させることが可能になる、エクソソームの検出方法を提供する。【解決手段】試料液１に、検出対象物質１２と反応する結合物質２２が結合した微粒子１２を含む緩衝液２を混合して、検出対象物質１２を有するエクソソームと微粒子１２とから成る結合体５を含む試料液７を生成し、検出対象物質１３と反応する結合物質４３が固定された基板３１上に試料液７を注入し、検出対象物質１３を有する結合体５を結合物質４３と反応させて基板３１上に結合体５を固定し、検出対象物質１４と反応する結合物質５４が結合した微粒子５０を含む緩衝液を注入して検出対象物質１２〜１４を有するエクソソームを固定した分析用基板を生成し、表面にレーザ光を照射して取得した検出信号が微粒子５０を示す信号であることを判定することにより、検出対象物質１２〜１４を有するエクソソームを検出する。【選択図】図１

光記録媒体

(57)【要約】 【課題】 繰り返し使用するとマスク材料が劣化し照射 光が弱いときでも透過率が高くなってしまったり、マス ク効果が小さくなってしまうことのない光記録媒体を提 供する。 【解決手段】 マスク層４を形成するサーモクロミック 材料の構成成分のうち、特に、電子受容性顕色剤として 特定の構造を持つビスフェノール系化合物のうちの少な くとも１種を使用した場合に、マスク層の繰り返し使用 回数が飛躍的に向上する。

光記録媒体

(57)【要約】 【課題】 電子供与性呈色化合物と電子受容性顕色剤と からなる２成分系サーモクロミック材料をマスク層とし て有する光記録媒体は良好なディスク超解像を示すもの の、反面、耐光性が十分でない。 【解決手段】 電子受容性顕色剤として、特定の構造を 有するビスフェノール系化合物のうち、少なくとも１種 を使用する。

光記録媒体

(57)【要約】 【課題】 電子供与性呈色化合物と電子受容性顕色剤と からなる２成分系サーモクロミック材料をマスク層とし て有する光記録媒体は良好なディスク超解像を示すもの の、反面、耐光性並びに耐熱性が十分でない。 【解決手段】 電子受容性顕色剤として、特定の構造を 有する３種の化合物のうち、２種以上を組み合わせて使 用する。

光記録媒体

(57)【要約】 【課題】 電子供与性呈色化合物と電子受容性顕色剤と からなる２成分系サーモクロミック材料をマスク層とし て有する光記録媒体は良好なディスク超解像を示すもの の、反面、耐熱性が十分でない。 【解決手段】 電子受容性顕色剤として、特定の構造を 有する化合物のうち、少なくとも１種を使用する。

Analysis device and analysis method

Analysis device and analysis method

洗浄装置及び洗浄方法

【課題】ウェルを洗浄した後に生じるウェル内の洗浄液の残留物を精度よく除去することができる洗浄装置を提供する。【解決手段】底面と内周面とを含む穴形状を有する複数のウェルが同一円周上に所定角度の等間隔に所定個数配置された分析用ユニットが装着されるステージと、ウェル内の溶液を吸引する吸引ノズルと、吸引ノズルがそれぞれ固定されている複数のノズルヘッドと、を備え、複数のノズルヘッドにそれぞれ固定された吸引ノズルは、複数のウェルに対して吸引ノズルの先端が内周面の近傍のそれぞれ異なる位置に配置され、制御部は、吸引ノズルの先端とウェルの底面との間を接近させた状態で、ウェル内の溶液を吸引ノズルにより吸引させた後、吸引ノズルの先端とウェルの底面との間を離隔させた状態で、分析用ユニットが装着されているステージを所定角度だけ回転させる動作を、ウェルの個数回おこなう。【選択図】図４

洗浄装置及び洗浄方法

【課題】ウェルを洗浄した後に生じるウェル内の洗浄液の残留物を精度よく除去することができる洗浄装置を提供する。【解決手段】洗浄装置１はステージ２とノズルヘッド１３と制御部３０とを備える。ステージ２にはウェル１３０を有する分析用ユニット１００が装着される。ノズルヘッド１３には、ウェル１３０内へ洗浄液ＣＳを吐出する吐出ノズル１１とウェル１３０内へ吐出された洗浄液ＣＳを吸引する吸引ノズル１２とを有する複数のノズルユニット１８が固定されている。制御部３０はステージ２を所定の角度単位で回転させる。複数の吸引ノズル１２はウェル１３０に対して互いに異なる位置に配置されている。制御部３０は分析用ユニット１００を所定の角度だけ回転させ、回転させた位置に対応する吸引ノズル１２で洗浄液ＣＳの残留物ＲＳを吸引させる。【選択図】図４

Method for analyzing biological sample

光ディスク収納ケース

【課題】長時間保管しても反りや変形を生じないように光ディスクを収納する光ディスク収納ケースを提供する。 【解決手段】光ディスク１を収納するケース本体５と、このケース本体５を蓋をする蓋体６とを有する光ディスク収納ケース４において、光ディスク１のセンターホールと同心円状に形成されている凸状のスタックリブ３に対応するケース本体５位置に凸状のスタックリブ３を嵌合させる円形状の凹部５Ａを形成して光ディスク収納ケースを実現した。 【選択図】 図２

光記録媒体

(57)【要約】 【課題】 色素記録層における記録マークとスペースと のコントラストを大きくして高密度に、高感度に且つ高 線速記録に適した高記録媒体を提供する。 【解決手段】 透明基板１上に少なくとも色素記録層 ２、反射層３及び保護層４を有する光記録媒体におい て、前記色素記録層が電子受容性顕色剤を含有した層で あり、かつ前記色素記録層に隣接して電子供与性呈色化 合物を含む呈色化合物層を設ける。これにより、レーザ 光Ｌが照射された部分の電子受容性顕色剤と電子供与性 呈色化合物との融解混合を生ぜしめて発色構造を形成 し、高密度に、高感度に且つ高線速記録に適した高記録 媒体とする。

磁石構造体、クランプ機構、及び光ディスク装置

【課題】磁石構造体の磁着力が大きくても、高いトルクを発生させるアシスト回路を設ける必要がなく、磁石構造体を被磁着体から容易に引き離すことができる磁石構造体を提供する。 【解決手段】磁石構造体１は、第１の磁石ユニット１０と第２の磁石ユニット２０とを備える。第１の磁石ユニット１０には、第１の面１２ａと第１の面１２ａ側を第１の極性とする第１の磁石１４とを有する第１の磁石固定部１２が形成されている。第２の磁石ユニット２０には、第２の面２２ａと第２の面２２ａ側を第１の極性の逆極性である第２の極性とする第２の磁石２６とを有する第２の磁石固定部２２が形成されている。第１の面１２ａと第２の面２２ａとは同一平面上に隣接して配置されて、被磁着体に磁着する磁着面１ｂを形成している。 【選択図】図１

分析装置、分析用基板及び分析方法

【課題】反応領域を特定して生体物質の分析を行う分析装置を提供する。【解決手段】分析装置１は基板６０が保持されるターンテーブル１０、ターンテーブル１０の回転軸Ｃ１０の直交方向に駆動され、反応領域６５にレーザ光２０ａを照射してその反射光を受光する光ピックアップ２０、光ピックアップ駆動回路８、及び制御部９を備える。基板６０は複数の反応領域が基板６０の回転方向に互いに離れた位置に形成され、かつ、最も基板６０の中心側に位置する第１の反応領域が他の反応領域とは径方向に異なる位置になるように形成され、ターンテーブル１０に保持される。光ピックアップ２０が、第１の反応領域における最も基板の中心側に位置するトラックにレーザ光を照射したときに包絡線パルス信号を生成したタイミングを基準として生成した測定ゲート信号毎に微粒子数をカウントし、各反応領域における微粒子の総数を計測する。【選択図】図６

光記録媒体

【課題】表面反射を防止しつつかつ生産の容易な光記録媒体を提供する。 【解決手段】光記録媒体１１は、記録再生光によって再生される情報を記録した情報ピット２が形成された信号面６と、信号面６と対向する側に前記記録再生光が入射される読み出し面７とを有する基材１を備える。ここで読み出し面７には、前記記録再生光の反射を低減する複数の反射防止構造体３が前記記録再生光の波長よりも小さいピッチで形成される。反射防止構造体３それぞれは前記読み出し面上７において凹んだ凹形状を有し、読み出し面７上における縁部の任意の２点を結ぶ線分のうち最長の長さとなる線分の長さが、底面の縁の任意の２点を結ぶ線分のうち最長の長さとなる線分の長さよりも長くなる。 【選択図】図１

Conjugate formation method, substance of interest measurement method, and kit