PROCEDURE FOR THE INCREASE THE PROTEIN AND AMINO ACID CONTENTS OF ANIMAL FEEDS

Already long ago one recognized the main factors of each nutrition of humans and animal in proteins, carbohydrates, fats, vitamins and mineral materials. Research work, which was accomplished in particular into the last 3ahrzebnten, to have new realizations over the connections of the factors mentioned brought in the nutrition, and successfully one tried to set up exact demand figures for the different organisms. During view first the physiology of monogastrischer organisms it lets itself state that proteins (and as if develop [emente the proteins the amino acids, above all the essential amino acids), carbohydrates and fats the three substantial macro components of the nutrition of these organisms are. While carbohydrates and fats can replace or supplement as sources of energy in limited measure each other, did not succeed until today replacing proteins and amino acids by other connections. It is well-known that Wiederkäuer can use also NichtProtein nitrogen (NPN) as protein replacement, but all attempts to reach something similar also with monogastrischen depths could furnish to today no clear and convincing results. For this reason of beruh% the fodder basing for monogastrische animals still on a mixture of different raw materials, which represent a corresponding entire to the nourish-physiological needs of the animals in their sum. Thus the usual fodder raw materials are divided into different groups, e according to that whether they are protein, Energieoder 8ohfaserträger. In the modern mixture fodder industry for example mainly following raw materials are used: as protein carriers: as sources of energy: as raw fiber carriers: Sojaschrot, fish flour, animal flours, yeast grain, fats of mill after products, Grasoder Luzernemehl global economic one regarded is to be stated that under the protein carriers Sojaschrot takes a key position, since the offer at Fischund animal flours is too expensive relatively limited and fodder yeast. The problem of the protein supply of many countries begins with that for climatological reasons the cultivation of soy beans is not possible and/or not economical, and that alternative products ffir the protein supply into vie! to small klengen for order, for this reason strive institutions for seeds seeds institutionseeds stand on the whole world since many years, sorts of grain on the market to bring, whatever exhibit a higher protein content than the usual sorts with good yields. Certain successes in this direction are to be registered, but all too frequently there were unwanted side effects, like e.g. bad amino acid balance, large sensitivity of the plants CD such. In the DE-OS 2024869 is a procedure for the processing of stärkebzw. kohlehydrathaltigen products under additive of non--protein substances (NPN substances) by anaerobic Vergärnng it described with which it comes only to a change of the chemical form of the NPN substance, whereby it is caused that these NPN substances for Wiederkäuer are no longer toxic. , The vergorene mass available in accordance with this disclosure writing is suitable excluding only for feeding of Wiederkäuern, since monogastrische animals cannot use NPN connections. Into another direction goes a fodder additive for monogastrisehe animals, which is descriptive in at-PS Nr.346165. “In vitro” adapted Pansenbakterien became with the help of special here with certain - rfolgen tried to put monogastrische animals into the position to use but certain quantities of NPN substances as protein replacement. The circumstance is however always given that each biological substance, which depths over the fodder is given, first a hostiles environment (in particular stomach Monogastria) happen must and for the protein synthesis only in the small intestine to the effect come can, passes and if one continues to consider that the taken up food in very short time the small intestine tract, is to be stated that also an optimally adapted Mikroflora will be able to convert only relatively small quantities urea in protein connections, the gegenständliche invention proceeds from new considerations, which are briefly summarized in the following: 1. In principle a protein synthesis is possible only if all elements, i.e. C, necessary for it, are N, O, H and S, present. s 2. it is well-known that the Pansenflora of Wiederkäuern is able to synthesize protein from NPN substances (bacterium). 3. For the protein synthesis by bacteria it is necessary that chemical energy is available in the form of ATP. 4. Nitrogen and hydrogen containing substances, e.g. urea, ammonia, hydrazine I0 and ammonium salt, hydrazine salts od.dgl, are such NPN connections. As possible form of one (bacteria: -) - protein synthesis appears according to invention a fermentation, which runs off approximately after the following characteristics; a) Urea, ammonia and/or other NPN connections split by microorganisms first and in particular into ammonia (also easily available nitrogen) umgeIS sets; the nitrogen of the latter is used, if the remaining elements of the proteins and ATP indicated above are present, for the increase of the biomass and thus (bacterium) the protein synthesis. b) The elements C, O, H as well as the ATP are supplied by the dismantling process using at the same time by strength and the sulfur necessary for the synthesis of the schwefelhaltigen amino acids e.g. by sulfates. c) It would be favorable to be able to let the two parallel run off under a) and h) of described processes without they disturb each other. In a large number of practical attempts it was observed that it can possible in principle be attained a simultaneous running off of the processes mentioned. This succeeds best if in no moment to large quantities of free ammonia in the mass are present and if on the other hand by too a Milchsäuregärung rapidly running off the pH value of the fermentation mass drops not prematurely under a pH value of 5, in particular from 6, since the NPN using microorganisms are active in particular in an environment, which lies in the proximity of the neutral range or over it. The subject of the invention is a procedure for the increase Rohproteinund amino acid contents of animal feeds, whereby stärkehältige and/or carbohydrate-rich substances and/or products, as for example corn or grain, under additive of Nichtproteinsubstanzen (NPN substances), as for example ammonia or urea, will anaerobic ferment, which are characterized by that the substances and/or products mentioned with a culture of “in vito” increased Paris micro organisms, preferably in combination with an additional culture of ammonia-resistant lactic acid bacteria, under additive at least a non--protein nitrogen (NPN) - substance in the pH range between 5,0 and ii ferments becomes. Preferred a culture is preferably used by preferably freshly slaughtered Wiederkäuern taken and microorganisms bred on a raw fiber-rich substrate, in combination with a ammonia-resistant culture of lactic acid bacteria, in vitro. The procedure according to invention differs from the procedure in accordance with DE-OS 2024869 thereby that in vitro increased Pansenmikroorganismen and favourably additionally specifically bred lactic acid bacteria are used that a genuine synthesis takes place from bacteria protein from NPN, whereby NPN is converted to a large extent into bacteria protein that after terminated fermentation the amino acid contents of the received mass lie substantially more highly than before the fermentation - e.g. an analysis certification of the agricultural resulted in chemical federal laboratory in Vienna an increase of 74 raw protein - and finally that the received vergorene mass is suitable to monogastrische animals for the feed. However with proceeding in accordance with DE-OS 2024869 none separated added Mikroorgauismenkulturen used, in particular no added Pansenmikroorganismen, if necessary in combination with ammonia-resistant lactic acid cultures, it is there not possible to synthesize a protein and/or a protein composed of amino acids. NPN changed in its chemical form in such a way that the substances for Wiederkäuer are no longer toxic, the procedure leads to no enlargement Proteinbzw. Amino acid content of the mass, and finally the vergorene “mass can not be able to use ausschließlioh only for Wiederkäuer feeding to be used, since monogastrische animals also the mentioned, in its chemical form somewhat changed NPN connections at all. The procedure according to invention means in a time, in which protein food for animal and humans becomes ever scarcer, by being capable from synthetic nitrogen suppliers large quantities of raw proteins of producing, an important progress. The pH value is kept favourable during the main process of the fermentation on values from 5 to 6.8. I0 it is here stressed that under local and temporal fluctuations in production of ammonia the pH value can reach values up to IL. Favorable way is led in such a way the fermenting process that in a first fermentation phase NPN first to ammonia is reduced and used afterwards as a N-donor for a bacteria protein synthesis, whereby gleiohzeitig Milchsäuregärung uses and in a second phase the Milchsäuregärung the expiration of fermentation dominated, so that vergorene the according to invention mass at the end of the fermentation exhibits a pH value between 4 and 6, preferably between 4 and 5. In accordance with a preferential variant, with which an exact observation of the pH process is unnecessary to a large extent, are intended that the pH value in the reaction environment by addition of a buffer, in particular on basis of a CIT advice, acetate, hydraulic gene) phosphate and/or (hydraulic gene) - carbonate of well, K, NH and/or alkaline earth metals are adjusted. Preferred for buffering NaHCO CaCO3 and/or Dicalciumphosphat is used. Apart from the relief of pH control in particular a too strong change of the pH value in the critical start time of the process according to invention is avoided by buffering. Advantage of the buffers mentioned is in particular that the elements contained in them are useful for the animal nutrition all together. As NPN substance favourably ammonia or urea, their salts and/or hydrazine, (Bi) - Guanidin, Biuret, Triuret and/or their salts, in particular their phosphates, carbonates, sulfates and/or nitrates, are used. Preferred urea and ammonia and/or their salts, which are present favourably in aqueous solution, are used. The procedure according to invention furnishes particularly favorable results, if the reaction mixture before the fermentation by addition of water on a moisture content from 30 to Gew. - %, in particular from 35 to 45 Gew. - one brings %. It can be worked however depending upon circumstances also at substantially higher water contents. The temperatures during the fermentation are held favourably within the range of 18 to 50°C, preferably from 20 to 40°C, in particular from 20 to 30°C. Usually, and this for economic reasons, becomes at the straight dominant environmental temperature fermented. The expiration of fermentation does not have a exergonischen character, it is thus during the fermentation of the carbohydrate-rich raw materials supply of heat energy from the outside necessary, since the fermentation mass warms up automatically, whereby temperatures up to 50°C in the fermentation mass can be achieved. Frequently the temperature lies during the main fermentation procedure with for instance 40°C and drops later. The quantity procedure in accordance with NPN substance, e.g. urea and/or ammonia which can be used, amounts to favourable from 1,5 to 10 Gew. - %, in particular from 2 to 4 Gew. - %, in each case related to the quantity of the Trockensubstanz of the char-hydrate-rich substrate which can be fermented. The biomass of the culture of in vitro increased Pansenorganismen becomes favourable in quantities from 0,3 to 2.0 Gew. - %, in particular of 0,5 to l Gew. - in each case related to the quantity of the Trockensubstanz of the char-hydrate-rich substrate which can be fermented, assigned. Those favourable-proves additionally to and/or in mixture with the ammonia-resistant lactic acid bacteria used in vitro increased Pansenmikroorganismen preferentially in quantities from 0,2 to 1.5 Gew. - %, in particular of 0,3 to I Gew. - %, in each case related to the quantity of the Trockensubstanz of the char-hydrate-rich substrate which can be fermented, assigned. - 5 - “Nr.365418 in the following is described now the expiration of the procedure according to invention and the preliminary stages on the basis a concrete execution form: 1. In “in vitro Vergärung in several stages under Beigabe of small in each case quantities of ammonia lactic acid cultures are increased in such a way that in the end a quantity of for instance i0 kg of a concentrate will receive from lactic acid bacteria, which have the characteristic to survive a preferably easily ammoniakales environment without damage. This product is called product “A”. 2. L0 kg of Panseninhalt freshly slaughtered Bindes are e.g. mixed with 50 kg of a raw fiber-rich substrate, Malzkeimen (by-product of the salt production), under addition of the product A descriptive by 0,5 kg urea and 0,3 kg ammonium sulphate as well as 1 kg under 1. So much water is added, until in the mass a Feuohtigkeitsgehalt is reached of approximately 45%, and this mass is given to a container, which is favourably hermetically locked and left untouched in an area with 40°C. In this way the besohriebene mass is bebrütet during at least 48 h, favourably up to 72 h or longer. The received product is named product “B”. (Note: The pH value of the mass very often already dropped after 72 h to a pH of, and the mass is stably, so long no air is added.) If this mass is to be stabilized, then it will become carefully - at a temperature of no more than 50°C - dried, 3. it 1000 kg of corn (or another grain) to grind, and from this the following mixture is manufactured: i000 kg of corn (grain) I0 kg calcium carbonate (fodder lime) kg of Dicaloiumphosphat 3 kg of Natriumbicarbonat kg urea kg ammonium sulphate kg product “B” (production described as under 2.) kg product “A” (production described as under 1.) it is brought so much water admitted, until a moisture content is reached from 35 to 45%, the mass into a silage silo, which is well taken off. It uses a fermentation, which runs off easily effort, u.zw favourably in the following form: During the first about three days, if necessary also somewhat longer, two biological procedures run off parallel: The Pansenbakterien converts the urea to ammonia and CO2; at the same time increases the Pansenmikroorganismen, which uses the nitrogen from ammonia as well as the elements C, O and H as well as ATP for the increase of the biomass, freed from the Glykolyse. The two biochemical procedures parallel running off are thus splitting the NPN substance, e.g. urea by Pansenbakterien and increase the same on the one hand and Milohsäuregärung on the other hand, open one the silo during the first three days, then one will notice a relatively strong ammonia concentration. At expiration that about three days is then practically final the dismantling of the urea, and the Milchsäuregärung continues to continue. The consequence of it is at the end of the process after seven to fifteen days dropping the pH value up to values of for instance 4. thereby is final the fermentation. Initially determined ammonia is missing any longer. In a concrete case this stage was reached after approximately fifteen days. The investigation of the vergorenen mass resulted in: Raw protein: Urea: Lysin: 17.2% in Trockensubstanz 0.05% in Trockensubstanz 0.75% in Trockensubstanz this analysis prove clearly that the urea up to small traces was actually converted to protein. It took place thus a protein synthesis and thus a protein proteinvermehrung, whereby it is to be particularly noticed that in particular also the Lysinbilanz of the mass was substantially improved. The analysis of the mass before beginning of the fermentation showed the following values: Raw protein (without urea) about 9%, Lysin for instance 0,20B. If one considers that into the modern feeding recommendation “n for the pig mast is regarded, in order to call an example a Bohproteingehalt of approximately 16 and a Lysingehalt of approximately 0.70 as sufficient, then the above attempt shows clearly that it makes the procedure according to invention possible to produce a perfectly balanced Schweinefutter only by the fermentation of grain - without possibly a protein additive -. Particularly interesting aspects result e.g. in the fermentation of wet corn: Since corn in many countries with a moisture content is harvested vor* more than 30%, it is possible in the procedure according to invention not to only conserve corn without drying process to ferment but in such a way according to invention that Rohproteinund amino acid contents is substantially improved. After relevant computations the fodder costs of pigs can be decreased by 20 to 40%, completely apart from substantial Deviseneinsparungen for imported goods by protein animal feeds with application of the procedure according to invention, as they must be accomplished by many countries. For the execution of the fermentation according to invention is to be noticed still in addition: The preparation of product “A” is relatively according to invention simple. In principle it is however possible, also one in any other form prepared to use however ammonia-resistant lactic acid bacterial culture and/or do without under certain conditions on it. According to invention the culture substantial for the fermentation is the product “B”, in which as high a concentration at Pansenmikroorganismen as possible is to be aimed at. By a constantly controlled production it is possible, the product “B” continuous by use of the finished product as Inokulum for new cultures, i.e. without adding again Panseninhalt to produce and to make the final product durable in each case by means of careful drying process. Preferential the subject of the invention is thus in particular also a procedure for continuous increase the procedure according to invention descriptive above by Pansenmikroorganismen for the employment in, which is characterized by the fact that the Panseninhalt taken by preferably freshly slaughtered Wiederkäuern is preferably blended with a raw fiber-rich substrate and with a ammonia-resistant culture by lactic acid bacteria and NPN substances and bebrütet under anaerobic conditions at a temperature of 30 to 45°C at least 48 h, in particular at least 72 h, long, whereby in the first phase a strong increase of the Pansenmikroorganismen takes place and uses in the following phase a Milchsäuregärung, which does not destroy the activity of the Pansenmikroorganismen, but only temporarily stops, around these during re-establishment one within the range of 5 to 11, in particular from 5 to B, 8, to bring lying pH value again to the development. P/ TENTANSPRÜCHE: 1. Procedure for the increase Rohproteinund amino acid contents characterized by animal feeds, whereby stärkehältige and/or kohlehydratreiehe substances and/or products, as for example bilais or grain, will anaerobic ferment under additive of non--protein substances (NPN substances), as for example ammonia or urea, by the fact that the substances and/or products mentioned with a culture of “in rode” increased Pansenmikreorganismen, preferably in combination with an additional culture of ammonia-resistant lactic acid bacteria, under addition at least a non--protein nitrogen (NPN) - substance in the pH range between 5,0 and II fermented become.