СПОСОБ УЛУЧШЕНИЯ ЦВЕТА МЯСА ЛОСОСЯ

Изобретение относится к изменению цвета мяса лосося. Способ предусматривает выращивание лосося на кормовом продукте, смешанном с каротиноидным красителем. Каротиноидный краситель включает, по меньшей мере, астаксантин, фоеникоксантин, кантаксантин и адониксантин. Содержание астаксантина в корме составляет от 20 до 100 мг/кг. Содержание фоеникоксантина в корме составляет от 5 до 80 мг/кг. Изобретение позволяет выращивать лосося, имеющего натуральный красновато-оранжевый цвет мяса при добавлении доступного натурального красителя в корм. 6 н. и 21 з.п. ф-лы, 14 табл.

Способ переработки крахмалосодержащих отходов картофеля

Изобретение относится к пищевой промышленности. Способ переработки крахмалсодержащих отходов картофеля для получения сухого кормового продукта характеризуется тем, что массу верхнего слоя клубней картофеля гомогенизировали до размеров 1-2 мм и содержания сухих веществ 13-15%, подвергали гидролизу ферментным препаратом глюкоамилазой в концентрации 2,0-5,0 г/кг в течение 90 мин при 60-65°С, что способствует обогащению среды глюкозой до 5,0% и активному росту дрожжей, гидролизат обогащали минеральными солями с концентрацией, %: (NN4)2SO4 - 0,15, (NH4)2HPO4 - 0,5, MgSO4 - 0,1, ZnSO4 - 0,05, в качестве продуцента белка использовали дрожжи штамма Saccharomyces cerevisiae ZS, посевной материал получали выращиванием в глюкозо-дрожжевой среде с аэрацией в течение суток при 30°С, норма внесения 10-15% от массы среды, дрожжи выращивали с аэрацией перемешиванием 250 об/мин в течение 18-24 часов при температуре 28-30°С до концентрации 5×108-1×109 КОЕ/г, дрожжеванный гидролизат подвергали высушиванию ...

СПОСОБ ПОЛУЧЕНИЯ ЗЕРНОВОГО КОРМА ДЛЯ СЕЛЬСКОХОЗЯЙСТВЕННЫХ ЖИВОТНЫХ

Изобретение относится к области животноводства, а именно к способу производства корма из целого или некондиционного зерна. Способ включает дозирование, увлажнение и перемешивание зернового сырья и его гидролиз при давлении 1,8-2,3 атм. В качестве зернового сырья используют целое или некондиционное зерно, которое подвергают гидролизу при температуре 135-140°C в течение 40-45 минут. Осуществление способа позволяет повысить содержание сахара и кормовую ценность корма. При осуществлении способа устраняются антипитательные свойства исходного сырья, уничтожаются плесневелые грибки, изменяется структура крахмальных зерен, что повышает пригодность корма к скармливанию животным. 2 табл., 3 пр.

Способ производства функционального кормового продукта для сельскохозяйственных животных

Изобретение относится к сельскохозяйственной промышленности, а именно к кормопроизводству. Способ производства кормового продукта для сельскохозяйственных животных, характеризующийся тем, что он предусматривает внесение молочной сыворотки в ферментер, добавление в нее питательных веществ и внесение подготовленных древесных опилок из березы или ели в соотношении опилки:сыворотка - 1:10, перемешивание, пастеризацию, а после пастеризации сыворотку с внесенными опилками и питательными веществами охлаждают до температуры 33±1°С, вносят культуру Lactobacillus и препарат БК-Углич-ПРО, содержащий Propionibacterium freudenreichii, проводят ферментацию до снижения рН 4,0-4,6, затем проводят ультразвуковую кавитацию смеси при установке выходной мощности генератора, соответствующей интенсивности ультразвука, равной 2.4 Вт/см2, далее смесь охлаждают до 30±1°С и вносят культуру кормовых дрожжей верхового брожения Saccharomyces cerevisiae и продолжают культивирование в течение 36-48 часов с аэрацией, ...

Способ получения белково-углеводной кормовой добавки

Изобретение предназначено для использования в кормопроизводстве для сельскохозяйственных животных. Способ получения углеводно-белковой кормовой добавки, включающий измельчение зернового сырья, внесение амилолитических и глюкоамилолитических ферментов и перемешивание с измельченным зерновым сырьем. Получение углеводно-белковой кормовой добавки проводят в следующей последовательности и следующих значениях влажности и температуры: доводят влажность овса и ячменя, взятых в соотношении 1:1, до 20-22% путем ввода расчетного количества воды равномерно и одновременно с началом измельчения. Затем измельчают зерносмесь до разрушения зародышей зерна при температуре 30-4°С. При уменьшении влажности продукта на 1,5-2,0% вносят амилосубтилин активностью EA – 1000 из расчета 0,1-0,15 г на 1 кг исходной смеси и перемешивают при снижении интенсивности измельчения. При влажности 16-18% и температуре продукта 37-40°С вносят глюкаваморин активностью ЕА - 1000 из расчета 0,15-0,22 г на 1 кг исходной смеси и ...

КОМПОЗИЦИЯ НА ОСНОВЕ КОЗЛЯТНИКА ВОСТОЧНОГО И СПОСОБ ЕЁ ПОЛУЧЕНИЯ

Изобретение относится к композиции на основе козлятника восточного и способу её получения. Композиция на основе козлятника восточного является механокомпозитом, состоящим из гидролизующего и/или солеобразующего компонента и высушенной травы козлятника восточного. В качестве гидролизующего и/или солеобразующего компонента композиция может содержать гидроксид натрия, или аскорбиновую кислоту, или ферментный препарат, проявляющий активность по отношению к козлятнику восточному, в качестве которого может быть выбран ферментный препарат, обладающий преимущественно целлюлолитической активностью. Способ получения композиции на основе козлятника восточного предусматривает смешивание высушенной травы козлятника восточного с гидролизующим и/или солеобразующим компонентом в соотношении (95-99):(1-5) по массе, соответственно. Полученную смесь подвергают механохимической обработке в мельнице-активаторе. В качестве мельницы-активатора используют проточные шаровые виброцентробежные, эллипсные центробежные ...

Способ приготовления кормовой добавки из птичьего помета с применением штаммов бактерий Bacillus subtilis ТНП-3-ДЕП и Bacillus subtilis ТНП-5-ДЕП

Изобретение относится к сельскому хозяйству и биотехнологии, в частности к производству кормовых добавок с использованием микроорганизмов. Для приготовления кормовой добавки помет кур-несушек после предварительного скармливания им пробиотика, состоящего из равного соотношения штаммов бактерий Bacillus subtilis ТНП-3-ДЕП и Bacillus subtilis ТНП-5-ДЕП в 1%-ном растворе глюкозы в концентрации 5×10КОЕ на 1 голову ферментируют пробиотиком, состоящим из штаммов Bacillus subtilis ТНП-3-ДЕП и Bacillus subtilis ТНП-5-ДЕП из расчета 5×10КОЕ на 100 г помета. Полученную массу подвергают инфракрасной сушке при температуре 90°С в течение 30 мин. Осуществление изобретения позволяет получить кормовую добавку с высоким содержанием аминокислот, питательных веществ и являющуюся микробиологически безопасной. 4 табл.

СПОСОБ ПОЛУЧЕНИЯ D-ПАНТОТЕНОВОЙ КИСЛОТЫ И/ИЛИ ЕЕ СОЛЕЙ, ИСПОЛЬЗУЕМЫХ В КАЧЕСТВЕ ДОБАВОК ПРИ ПРОИЗВОДСТВЕ КОРМОВ ДЛЯ ЖИВОТНЫХ

... 1. Способ получения D-пантотеновой кислоты и/или ее солей, отличающийся тем, что a) используют, по меньшей мере, один организм, продуцирующий D-пантотеновую кислоту, в котором нарушена регуляция биосинтеза пантотеновой кислоты-(pan) и/или изолейцина/валина-(ilv), и который в процессе ферментации в культуральной среде производит, по меньшей мере, 2 г/л соли D-пантотеновой кислоты, причем в культуральную среду добавляют 0-20 г/л свободного β-аланина и/или солей β-аланина, b) к образовавшемуся D-пантотенату добавляют соли, содержащие многовалентные катионы, в результате чего образуются многовалентные соли D-пантотеновой кислоты, c) раствор, содержащий многовалентные соли D-пантотеновой кислоты подвергают нанофильтрации, в результате чего концентрация многовалентных солей D-пантотеновой кислоты увеличивается, и d) ретентат, полученный после нанофильтрации, содержащий многовалентные соли D-пантотеновой кислоты подвергают затем сушке и/или формовке. 2. Способ по п.1, отличающийся тем, что в культуральную ...

Способ кормления цыплят-бройлеров

Изобретение относится к сельскому хозяйству. Способ кормления цыплят-бройлеров включает кормление полнорационным комбикормом, причем с 7-дневного возраста к основному ежедневному рациону, перемешивая с комбикормом, вводят молочно-растительную кормовую добавку на основе гидролизованного дрожжами Saccharomyces cerevisiae яблочного жмыха в количестве 5% от общего объема рациона с последующим скармливанием в течение всего периода выращивания. Изобретение позволяет повысить продуктивность и иммунную функцию птицы, улучшить качественные показатели мяса, снизить процент заражения птицы Salmonella enteritidis. 6 табл.

Способ получения кормового белкового продукта

Изобретение относится к комбикормовой промышленности, в частности к способу получения белковой кормовой добавки из древесных отходов. Способ включает предподготовку древесного сырья, его гидролиз и выращивание на гидролизате микроорганизмов. Опилки лиственной древесины, измельченные и при этом механоактивированные, гидролизуют в водном растворе гидроксида калия при рН (11±1) в акустическом поле широкополосного гидроакустического излучателя с плотностью акустической энергии 0,3 Вт⋅с/смв течение 2 часов. Добавляют ~10% отрубей, инокулируют консорциумом микроорганизмов Fusarium sambucinum + Trichosporon cutaneum + Saccharomyces cerevisiae и выращивают в течение 24-48 часов. Использование изобретения позволит получить высокобелковую кормовую добавку из древесного сырья. 1 табл., 3 пр.

СПОСОБ ИСПОЛЬЗОВАНИЯ ТОПИНАМБУРА В ПЕРЕПЕЛОВОДСТВЕ

СПОСОБ ПОЛУЧЕНИЯ D-ПАНТОТЕНОВОЙ КИСЛОТЫ И/ИЛИ ЕЕ СОЛЕЙ, ИСПОЛЬЗУЕМЫХ В КАЧЕСТВЕ ДОБАВОК ПРИ ПРОИЗВОДСТВЕ КОРМОВ ДЛЯ ЖИВОТНЫХ

... 1. Способ получения D-пантотеновой кислоты и/или ее солей, отличающийся тем, что a) используют, по меньшей мере, один организм, продуцирующий D-пантотеновую кислоту, в котором нарушена регуляция биосинтеза пантотеновой кислоты-(pan) и/или изолейцина/валина-(ilv), и который в процессе ферментации в культуральной среде производит, по меньшей мере, 2 г/л соли D-пантотеновой кислоты, причем в культуральную среду добавляют 0-20 г/л свободного β-аланина и/или солей β-аланина, b) ферментационный раствор, содержащий D-пантотенат, пропускают через колонку с анионообменной смолой, c) связанный с анионообменной смолой D-пантотенат элюируют в виде свободной D-пантотеновой кислоты раствором, содержащим неорганические или органические соли кальция и/или магния в виде D-пантотената кальция и/или магния, или раствором соляной кислоты, d) при необходимости, рН элюата, содержащего свободную D-пантотеновою кислоту, добавлением кальциевых и/или магниевых оснований доводят до значения 3-10, при этом получают ...

Food product compositions and methods for producing the same

PROCEDURE FOR THE PRODUCTION OF D-PANTOTHENSÄURE ONES AND/OR THEIR SALTS AS ADDITIVE FOR ANIMAL ANIMAL FEEDS

VERFAHREN ZUR HERSTELLUNG VON ENERGIELIEFERNDEN ROHSTOFFEN AUS ABFALLSTOFFEN, WII MUELL, KLAER- SCHLAMM UND KOMPOSTEN

VERFAHREN ZUR HERSTELLUNG EINES STABILEN KONZENTRATS MIT HOHEM GEHALT AN ESSENTIELLEN BIOFAKTOREN

PROCEDURE FOR THE INCREASE THE PROTEIN AND AMINO ACID CONTENTS OF ANIMAL FEEDS

PROCEDURE FOR THE PRODUCTION OF D-PANTOTHENSÄURE ONES AND/OR THEIR SALTS AS ADDITIVE FOR ANIMAL ANIMAL FEEDS

ANIMAL FEED PRODUCT

Method of improving salmon meat color

It is intended to provide a method of improving salmon meat color by which a salmon having a mild reddish orange meat color similar to natural ones can be obtained; a feed for improving meat color; and a salmon and its meat obtained by the method of improving salmon meat color as described above. It is also intended to provide a method of obtaining a salmon having a mild reddish orange meat color characterized by comprising feeding the salmon with a feed containing a carotenoid dye containing at least astaxanthin, phoenicoxanthin, canthaxanthin and adonixanthin.

Nucleotide sequences which code for the metd gene

TREATMENT OF ANIMAL WASTE TO PRODUCE MATERIALS FOR USE IN FODDER AND ON THE LAND

... . A method of treating animal waste arising in animal husbandry to obtain products useful on the land or in animal feedstuff, which comprises contacting freshly produced animal dung and urine with a sufficient proportion of a base sufficiently soluble therein and selected from alkali metal hydroxides, alkali metal carbonates, alkaline earth metal hydroxides, and mixtures thereof, to form an alkaline slurry having a pH value of from 9.0 to 12,0. The alkaline slurry is retained at said pH for a period of time sufficiently long to inactivate at the temperature of retention effectively all pathogens present, to yield at least one sterile product applicable to land as fertiliser or soil conditioner. At least part of said resulting sterile product is preferably subjected to aerobi? microbiological digestion optionally with nutrient additions influencing the balance therein of carbon, hydrogen, nitrogen, phosphorus and potassium to yield an effectively pathogen-free biomass applicable as an animal ...

PROCESS OF PREPARING A BIOMASS

A biomass which is made of a mixed culture of microorganisms is produced by bio-degradation of waste liquor from bisulfite pulping. Under non-aseptic conditions, and using nutrients with the liquor, the latter is treated with acclimatized microorganisms which are derived from sewage. The biomass which is obtained as a result of the multiplication of the microorganisms on the liquor can be separated, dried and pulverized to give a substance which can be used as feed for animals.

ANIMAL FEED SUPPLEMENT

COMPOSITIONS COMPRISING FERMENTED SEAWEED AND/OR ALGAE

The present invention relates to compositions comprising fermented seaweed and/or algae. The invention also relates to processes for providing such compositions, wherein the fermentation times are prolonged to increase bioavailability of the nutrients.

Procédé pour la préparation d'une nourriture pour bestiaux, digestive et de grande valeur nutritive.

Verfahren zur Herstellung von Futter- oder Nahrungsmitteln aus minderwertigen animalischen oder vegetabilischen Produkten.

Verfahren zur Herstellung eines Futtermittels.

Verfahren zur Herstellung von Dispersionen aus Extrakten vegetabilischer Herkunft.

Verfahren zur Herstellung eines vitaminhaltigen, die Fresslust anregenden Futtermittels

Verfahren zur Herstellung von Melassefutter.

Verfahren zur Herstellung haltbarer Produkte aus nichtsterilen, insbesondere dauersporenhaltigen Ausgangsstoffen.

Procédé de préparation d'un aliment pour les animaux domestiques, et aliment préparé par ce procédé.

High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration

Food- and feedstuffs with high protein content are prepd by culturing micro-organisms pref. Saccharomyces fragilis in a liq. nutrient medium which contains carbohydrates and dissolved protein such as obtd from molasses or whey from the cheese-making process. After culturing, the medium is ultra-filtered which is pref. carried out in two stages with a water-wash in between to remove salts. The process does not require the use of separators, gives a product with a higher protein content than previously obtd, and the yeast used has a cleaning action on the membranes of the ultracentrifuge.

High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration

Food- and feedstuffs with high protein content are prepd by culturing micro-organisms pref. Saccharomyces fragilis in a liq. nutrient medium which contains carbohydrates and dissolved protein such as obtd from molasses or whey from the cheese-making process. After culturing, the medium is ultra-filtered which is pref. carried out in two stages with a water-wash in between to remove salts. The process does not require the use of separators, gives a product with a higher protein content than previously obtd, and the yeast used has a cleaning action on the membranes of the ultracentrifuge.

Verfahren zur Herstellung haltbarer Gemüsesäfte unter gleichzeitiger Gewinnung von Sauerfutter

Verfahren zur Herstellung von granuliertem Futtermittel

High protein food- and feedstuffs prodn - by culturing micro organisms in protein-contg medium and ultrafiltration

Food- and feedstuffs with high protein content are prepd by culturing micro-organisms pref. Saccharomyces fragilis in a liq. nutrient medium which contains carbohydrates and dissolved protein such as obtd from molasses or whey from the cheese-making process. After culturing, the medium is ultra-filtered which is pref. carried out in two stages with a water-wash in between to remove salts. The process does not require the use of separators, gives a product with a higher protein content than previously obtd, and the yeast used has a cleaning action on the membranes of the ultracentrifuge.

Fermentation treatment for utilising pig excrement

A process is claimed for utilising pig excrement by fermentation, in which a loose, layered, air-permeable pile is first formed comprising layers of straw laden with solid pig faeces alternate with layers of straw soaked in pig urine. A heap of such layered piles is formed in a fermentation tower where fermentation is carried out at 40-60 degrees C.Excess liquid excrement left over from building the tower is percolated over the top of the heat so that it filters down through the pile. Excess liquid emerging from the base of the heap is caught in a collection tank and recycled to the top of the pile. A stream of air is led into the lower from the base.Process allows pig excrement to be treated safely and without the expenditure of large amounts of energy. There is no danger of pollution and contamination of surface waters. After fermentation, the prod. can be sterilised and dried for use as a protein supplement for animal feeds.

Poultry food contg. zeaxanthin and vitamin=B12

Food for poultry is prepared by (a) culturing a variety of Flavobacter microorganism, known produce zeaxanthine, in an aq. medium contg. a carbohydrate as carbon source, a nitrogen source which is a free amino acid or its mineral salts, trace elements, and vitamins, together with 200-1000 ppm by wt., of an iron salt and 2-100 ppm, by wt. of a cobalt salt; and (b) recovering the cellular mass.The food contains zeaxanthine which improves the colour of the animals skin and egg yolks, and it also contains appreciable quantities of vitamin B12. Addition of the iron and cobalt salts doubles the quantity of B12 in the product. The medium pref. contains 0.1-15% sucrose or glucose, 0.1-8% yeast extract, corn steep liquor or protein hydrolysate, and 0.1-2% magnesium sulphate.

PROCEDURE FOR THE KOMPOSTIEREN OF GANG PROPERTY FROM ORGANIC WASTES AND/OR SEWAGE SLUDGE IN TWO VERFAHRENSSTUFEN.

Germinated grain mixture

The germinated grain mixture comprises germinated and fermented cereal and leguminous plant grains, and is preserved in the moist state by seeding with lactic bacteria. It is used as a feed for farm animals.

Milk-replacer supplement feed and method for fattening calves for slaughter

When the exclusively dairy food for battery veal for slaughter is supplemented with a milk-replacer supplement feed, consisting of germinated cereal grains kept in a moist state, seeded with lactic bacteria selected for their production of lactic acid and their preserving effect, superior technical and economic performance levels, fattening safety and a normally white meat are obtained. The grains mentioned as milk-replacer supplement feed are described.

Process for the preparation of a biological product for stimulating vitality of humans and animals

The biological product for stimulating vitality of humans and animals based on a bacterial culture liquid is prepared by fermentation of a nutrient medium which contains sources of carbon and nitrogen and mineral salts with a bacterial ferment. The latter is a complex of thermophilic bacteria of the genus Lactobacterium, Streptobacterium, and mesophilic bacteria of the genus Streptococcus, Azotobacterium shermanni, Bacterium acidipropionicum, Escherichia coli and Pseudomonas.This complex results from symbiosis of the said microorganisms and adaptation thereof to the nutrient medium. The fermentation is carried out at a temperature from minus 5 to plus 15 DEG C for 24 to 36 hours and then at a temperature from plus 20 to plus 50 DEG C for 24 to 36 hours. The described product can be used in the food industry and in agriculture for activating the vitality of humans and animals.

PROCEDURE FOR THE PRODUCTION OF ANIMAL AND/OR VEGETABLE NUTRIENT FROM ORGANIC WASTE.

METHOD FOR PRODUCING MIXTURE OF D-PANTOTHENIC ACID AND SALTS THEREOF

Способ получения кормового белкового продукта из зернового сырья

Изобретение относится к биотехнологии, микробиологической и комбикормовой промышленности, в частности к способу получения кормового белкового продукта из зернового сырья. Способ, согласно изобретению, включает размалывание зернового сырья до размера частиц 60-160 мкм, смешивание с электролизованной водой с pH 5,0-6,0 при соотношении 1:8, термическую обработку при температуре 50°C и 95°C в течение 40 и 60 мин. соответственно, охлаждение, добавление 0,5% сульфата аммония, 0,0005-0,001% сквалена и 10% об. суспенсии дрожжей Saccharomycopsis fibuligera Y-436, Y-310 или Y-3875, культивирование в аэробных условиях при температуре 28°C в течение 24-48 часов, с последующей сушкой полученного белкового продукта. Результат изобретения состоит в ускорении роста дрожжей, увеличении биомассы в 1,4-2,3 раза, общей массы продукта в 1,2-1,4 раза и содержания белков на 7,0-23,5%. П. формулы: 2 ...

VERTICAL METHOD IDEAL DISPLACEMENT FOR SIMULTANEOUS PRODUCTION OF ETHANOL AND FERMENTED SOLID PRODUCT CONVERSION OF SUBSTRATE

PROBIOTIC FERMENTED FODDER ADDITIVE

Preparation method of high-palatability pig feed

Method for breeding cattle in large scale

Compound feed for young Mastacembelus armatus

Snakehead feed and preparation method of snakehead feed

Pig feed and preparation method thereof

Feed for improving immunity of cows/bulls and sheep/goats in winter and preparation method thereof

Fodder for improving quality of ass meat and preparation method of fodder

Feed produced from pectin-extracted orange peel pomace and production technology thereof

Artificial bamboo rat breeding method

New technology of extracting puerariae extract from wild pueraria

Bee larva probiotic oral liquid for animal healthcare

Method for preparing eucommia seed oil

Feed taking pig blood as raw material and preparation process thereof

Biological nutrient enzyme for aquatic products, and preparation method and usage method thereof

Preparation method of silage

Black wild pig breeding method

Piglet feed prepared by microbial fermentation and preparation method thereof

Feed formula capable of increasing nutritional, nutritious and medicinal values of black-bone sheep and preparation method thereof

Disease-resistant pig feed and preparation method thereof

Bioactive livestock feed based on selenium-enriched raw material

Compound feed additive used for poultry

Fermented seed liquid for fermented soybean meal, slow-speed fermented soybean meal prepared from fermented seed liquid and preparation method thereof

Ecological method for mixed cultivation of river crabs and toads in rice field

A method for utilizing of preparing feed additive producing artificial soil seeding for the method of the substrate

Fermented feed device, system and method

Goat breeding method

Microorganism feed with animal wastes as main raw materials and preparation method thereof

The invention discloses a microorganism feed with animal wastes as main raw materials and preparation method thereof; each constituent of the feed after anaerobic fermentation is as follows: 14.4 parts of crude protein, 9.4 parts of crude fat, 13.8 parts of crude fiber, 15.4 parts of crude ash, and 38.8 parts of nitrogen free extract. The technique comprises fecaluria deodorizer, pig feed transformed from cow shit, pig feed and cattle feed transformed from pure chicken manure, and bio-fertilizers made from leftovers. Such synthesis technique is not existed in nation.

Method for extracting melanin from rapeseed meal and application of precipitates obtained by method

A Chinese herbal medicine additive for livestock and poultry and a preparation method and application thereof

Fermented feed for improving pork quality as well as preparation method and application thereof

Chinese herbal medicine feed additive and preparation method thereof

Water conditioning agent for aquaculture

Feed for intercropping snail, grass carp and loach and preparing method thereof

Preparation method of pig feed

Attracting feed for accelerating growth speed of yak cubs and preparation method thereof

Intermediate-stage compound pig feed containing fermented snow pear juice and cottonseed dregs fermented by Lactarius deliciosus

Starter feed for egg-laying chick and making method thereof

Processing method of black pig breeding feed

Feed for preventing edema of piglets and preparation method of feed

Preparation process of livestock feed based on microbial fermentation

Pig feed additive and preparation method thereof

Feed additive for increasing average daily gain of pig herd

Deep processing device for feed for red elephant grass

High resistance bacillus subtilis and application thereof

Method for preparing protein feed additive by solid state fermentation plant protein feed

Preparation method of suckling pig yogurt feed

Mixed aquaculture method for grouper and blue-shelled turtle circulating water

Additive for improving organism immunity of young livestock as well as preparation method and application of additive

Preparation method of stomach strengthening feed

Process to prepare a low-sodium salt product, product obtainable thereby and the use thereof

The present invention relates to a process to prepare a salt product containing sodium chloride (NaCl) and at least one additive, wherein the salt product has a particle size of from 50 μm to 10 mm, which process comprises the steps of: a) optionally, crushing a sodium chloride-containing material to a particle size that is between 1,000 times smaller and 3 times smaller than the size of the final salt product; b) optionally, crushing the at least one additive starting material to a particle size that is between 0.5 and 2 times the particle size of the sodium chloride-containing material particles resulting from step a.); c) subsequently, mixing the sodium chloride-containing material particles of a particle size that is between 1,000 times smaller and 3 times smaller than the size of the final salt product, and additive particles of a particle size that is between 0.5 and 2.0 times the particle size of the sodium chloride-containing material particles; d) subsequently, compacting the particle mixture resulting from step c.) using a pressure of from 40 to 400 MPa; e. subsequently, crushing the compacted salt product to give particles of the desired particle size of 50 μm to 10 mm; wherein the steps are carried out under substantially dry conditions. Additionally, the invention provides the low sodium salt product obtainable by the process and the use thereof for human or animal consumption.

Food Production System From Biomass With Heat And Nutrient Recovery

A food production system having improved heat and nutrient recovery is disclosed. The system employs waste biomass and comprises a composter that partially composts the waste biomass, an invertebrate (e.g. worms) culture unit, a delivery subsystem to deliver partially composted waste biomass from the composter to the invertebrate culture unit in a temperature range suitable for the invertebrate culture, a food (e.g. fish or crustaceans) culture unit for producing the food operating in a temperature range suitable for the food culture, a delivery subsystem to deliver invertebrates from the invertebrate culture unit to the food culture unit, and a heat exchange subsystem providing for exchange of heat from the composter to the food culture unit. The subsystem comprises a controller for controlling the exchange of heat such that the food culture unit is maintained in the temperature range selected for the food culture.

Production of alcohol esters and in situ product removal during alcohol fermentation

An alcohol fermentation process and composition that includes production of alcohol esters by esterification of product alcohol in a fermentation medium with a carboxylic acid (e.g., fatty acid) and a catalyst (e.g., lipase) capable of esterifying the product alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the product alcohol recovered from the alcohol esters. The carboxylic acid can also serve as an extractant for removal of the alcohol esters from the fermentation medium.

Process for modifying protein digestion of ruminant feeds and products produced therefrom

Processes for increasing rumen undegraded protein in protein containing compositions, fermentation by-products or combinations thereof are disclosed. Uses of alkaline crystalline solids to increase rumen undegraded protein in protein containing compositions, fermentation by-products or combinations thereof are further disclosed. Products produced from such processes are also disclosed.

Fermentation process to produce natural carotenoids and carotenoid-enriched feed products

Methods of fortifying animal feeds nutrients typically lacking therefrom through the action of microorganisms are provided. Fermentation of animal feed materials with selected microorganisms, such as certain varieties of red yeasts, are shown to fortify the feed materials with natural carotenoids. Further, in certain embodiments, these methods can result in an increase in levels of nutritionally beneficial fatty acids, and a decrease in dietary fiber and nitrogen levels.

Method for desalting animal tissue

The present invention relates to a method for desalting tissue originating from animals. In particular, the present invention provides a method for desalting mucosa tissue, notably mucosa tissue originating from bovine or porcine. The invention also provides a method for preparing animal feed supplement or additive enriched in nutritional elements readily available to the animal, in particular to the young animal, for example young piglets, chickens, calves or aqua species. The invention also provides a low-salt content or a salt-free animal feed supplement and a feeding or growing method using the same.

Methods and compositions for increasing plant disease resistance and yield

The present invention discloses novel plant homologs of the Arabidopsis peptide atPEP1. atPEP peptides in Arabidopsis are involved in the amplification of defense pathways involved in innate immunity against microbial pathogens. Homologs to atPEP1 identified in soy, corn, rice, wheat, and canola sequence databases are potential sources for transgenes to enhance crop yield through resistance to biotic and/or abiotic stresses. Chimeric genes comprising sequences from mature and precursor plant defense response polypeptides from a given species, and from different species, as well as the encoded polypeptide sequences, are also described.

ENHANCED AEROBIC FERMENTATION METHODS FOR PRODUCING EDIBLE FUNGAL MYCELIUM BLENDED MEATS AND MEAT ANALOGUE COMPOSITIONS

Provided herein are shelf-stable protein food ingredients, food products comprising the shelf-stable protein food ingredients, methods of their production, and methods of their use. The shelf-stable protein food ingredients comprise cultured fungal biomass and a limited amount of water. Advantageously, the shelf-stable protein food ingredients can be stored, transported, and delivered within the food supply. 170-. (canceled)71. A method of producing a meat analogue food product from a filamentous fungal mycelium , wherein the method comprises:{'i': 'Neurospora', 'a) culturing a filamentous fungi from the genus in a liquid growth medium to produce a filamentous fungal biomass slurry comprising about 0.5-8% biomass;'}b) harvesting the filamentous fungal biomass slurry and dewatering the filamentous fungal biomass slurry to produce a harvested filamentous fungal biomass comprising about 60-85% water and about 15-40% filamentous fungal biomass;c) dewatering the harvested filamentous fungal biomass to produce a filamentous fungal biomass cake;d) producing filamentous particles from the filamentous fungal biomass cake and drying the filamentous particles;e) sizing the dried filamentous particles to comprise a mean particle size between about 5 mm and about 50 mm; andf) hydrating the sized dried filamentous particles at about 30% to about 70% water content to form the meat analogue food product.72. The method of claim 71 , comprising drying the filamentous particles to about 4% to about 10% water claim 71 , or to about 4% to about 6% water.73. The method of claim 71 , comprising drying the filamentous particles at about 50° C. to about 100° C.74. The method of claim 71 , comprising sizing the dried filamentous particles to comprise a mean particle size between about 5 mm and about 20 mm.75. The method of claim 71 , wherein the harvested filamentous fungal biomass is pasteurized before drying.76. The method of claim 71 , further comprising pasteurizing the meat analogue ...

METHOD AND PROCESS FOR ACIDIC RECYCLING OF PROTEIN WASTE

A method for recycling protein waste and producing an edible product for animal consumption comprises grinding animal carcasses or parts thereof and thereafter acidifying the waste. The acidified waste may then be emulsified, followed by a heat shock and then fed to animals. Alternatively, a second emulsification may be employed prior to being used for animal consumption. The emulsified products may be provided to animals as a liquid, or dried to a paste prior to use. 1. A process for making a product for animal consumption , said process comprising:a) Grinding a protein source with a carrier to generate a ground protein/carrier product;b) Adding acid to the ground protein/carrier product at a temperature between about 90 degrees Fahrenheit and about 120 degrees Fahrenheit to generate an acidified ground protein/carrier product;c) Mincing and maintaining the temperature of the acidified ground protein/carrier product between about 90 degrees Fahrenheit and about 120 degrees Fahrenheit at pH between about 2.5 and about 4.0 and allowing said ground protein to mostly liquefy to form an acidic digest medium exhibiting biological stability in a sealed container for at least 21 days.2. The process of claim 1 , further comprising:mixing an amount of the acidic digest medium with an additional protein source to form an acidic mixture, followed by a heat shock, and emulsification.3. The process of further including a drying step after emulsification. This application is a continuation of U.S. application Ser. No. 15/200,757 which was filed on Jul. 1, 2019, which was a Continuation-In-Part of U.S. application Ser. No. 13/563,214 filed Jul. 31, 2012, which is a continuation in part of U.S. application Ser. No. 12/925,359 filed on Oct. 20, 2010 (which issued as U.S. Pat. No. 9,161,554 on Oct. 20, 2015), which is a Continuation of U.S. application Ser. No. 11/706,123 filed Feb. 14, 2007 (which issued as U.S. Pat. No. 7,851,210 on Dec. 14, 2010), which is a Continuation-In-Part ...

METHOD FOR PRODUCING FEED FROM SECONDARY RESOURCES FROM THE RICE PROCESSING INDUSTRY

Technical field: the invention relates to the field of agriculture, and more particularly to the production of feeds from non-fodder raw material, specifically waste from the rice processing industry. Aim: to expand the range of raw materials for producing feeds for farm animals, in particular cattle, and to produce a nutritious feed from secondary resources from the rice processing industry not hitherto used for this purpose, which can be used as a complete feed and also as a novel ingredient in the production of compound feed for cattle. Essence of the invention: a method is proposed for producing feeds from rice processing industry waste, which involves grinding a plant matter and subjecting same to biofermentation treatment using an active culture of Trichoderma lignorum, wherein the main plant matter used is rice hulls and rice flour, which is ground to 0.2-0.5 mm and then hydrothermally treated at a temperature of 110-120° C. to a moisture content of 50-60% at a pressure of 1-2 atm for 1.5-2 hours, whereupon a biofermentation mass (“Trichoderma lignorum 19”) is added to the plant matter in an amount of 5-15% of the mass of the raw material undergoing processing, the mixture is directed to a sealed bin for implementation of a microbiological process of impregnation and build-up of protein mass for 24-48 hours, then the resulting biomass is dried at a temperature of 50-60° C. to 13-14.5% moisture content to produce a finished product. 1. The method of manufacture of the forage , including crushing of vegetation component and biofermented processing by the active culture of Trichoderma lignorum differs by that the rice peeling and hulling bran are used as vegetation component , which is crushed into powder with grain size of 0.2-0.5 mm , then , the hydrothermal processing at the temperature of 110-120° C. till humidifying up to 50-60% under the pressure of 1-2 atm. within 1.5-2 hours , then the biofermented mass (“Trichoderma lignorum 19”) in the quantity of 5-15 ...

VERTICAL PLUG-FLOW PROCESS FOR BIO-CONVERSION EMPLOYING MICROORGANISMS

The invention relates to a method for producing a solid transformation product of a substrate comprising the following steps: • preparing a substrate of biomass comprising carbohydrates and proteinaceous matter that originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, • mixing said substrate with a live microorganism or a combination of live microorganisms, which live microorganism or mixture of live microorganisms is not live yeast, and adding water in an amount which provides an initial incubation mixture having a water content from 30 to 70% by weight, and a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; • incubating said initial incubation mixture for 1-240 hours at a temperature of 15-70° C.; and thereafter recovering wet solid transformation product from the incubation mixture; further comprising that the incubating step is performed as a continuous plug-flow process in a vertical, non-agitated incubation tank with inlet means for said mixture and additives and outlet means for said solid transformation product. 134-. (canceled)35. A method for producing a solid transformation product of a biomass substrate , wherein the solid transformation product is a product of the transformation of one or more of proteinaceous matter and carbohydrates originating from a biomass substrate , comprising:(a) preparing a substrate of a biomass comprising carbohydrates and proteinaceous matter that originate from soya bean seed, rape seed, or mixtures thereof, wherein at least 20% by weight of said biomass comprises carbohydrates and proteinaceous matter originating from soya bean seeds, rape seeds, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from one or more of seeds of fava beans, seeds of peas, sunflower ...

BIOLOGICAL FORMULATION FOR COMPLEMENTING AN ANIMAL FEED, PROCESS FOR OBTAINING A BIOLOGICAL FORMULATION FOR AN ANIMAL FEED AND AN ANIMAL FEED

The present invention refers to a biological formulation for complementing an animal feed, which comprises: dry cells of or bacteria, containing at minimum 40% of PHB and B vitamins, tyrosine, glutamic acid, phosphates and iron, which formulation may further contain the endogenous PHB depolymerase enzyme. The process for obtaining the formulation comprises the steps of: (a) producing, by aerobic fermentation, from 32 to 34° C., in a mineral and aqueous fermentative medium containing sucrose, glucose or other sugary streams derived from sugar production, cells of or bacteria containing at minimum 40% of PHB and B vitamins, tyrosine, glutamic acid, phosphates and iron; (b) inactivating the cells, thermally, at a temperature from 65 to 90° C. for 15 minutes; and (c) drying the bacteria cells obtained in the previous step. 1Cupriavidus necatorAlcaligenes latus. A biological formulation , for complementing an animal feed , it which comprises dry cells of or bacteria , vitamins , tyrosine , glutamic acid , phosphates and iron.2. The biological formulation claim 1 , according to claim 1 , further comprising endogenous PHB depolymerase enzyme.3. The biological formulation claim 1 , according to claim 1 , comprising ruptured dry cells.4. The biological formulation claim 3 , according to wherein the ruptured dry cells present a PHB with a molecular weight less than 100.000 Da.5. The biological formulation claim 3 , according to claim 3 , characterized in that the dry cells present a pH from 6 and 8.6. A process for obtaining a biological formulation for an animal feed claim 3 , which comprises the steps of:{'i': Cupriavidus necator', 'Alcaligenes latus, 'a—producing, by aerobic fermentation, from 32 to 34° C., in a mineral and aqueous fermentative medium, containing sucrose, glucose or other sugary streams derived from sugar production, cells of or bacteria containing at minimum 40% PHB and B vitamins, tyrosine, glutamic acid, phosphates and iron.'}b—inactivating the cells, ...

PREPARATION METHOD FOR FERMENTED CORN GLUTEN

The present invention relates to a method of preparation for fermented corn gluten to improve the quality of corn gluten, which is a vegetable protein source, to a fermented corn gluten prepared by the method, and to a feed additive including the same. The present invention provides high-quality fermented corn gluten whose characteristics are improvement as a protein feed by inoculation of corn gluten, which has a high protein content but is minimally used due to low digestibility, with a sp. strain and solid-culturing the sp. strain, and a method of preparation therefor. 1. A method of preparation for fermented corn gluten with an increased protein content and improved digestibility compared to before fermentation , comprising:{'i': 'Bacillus', '(a) inoculating moisture-containing corn gluten with a sp. strain; and'}{'i': 'Bacillus', '(b) solid-culturing the sp. strain with which the corn gluten is inoculated to obtain fermented corn gluten,'}wherein the corn gluten to which the moisture is added has a moisture content of 40 to 70% (v/w), and{'i': 'Bacillus', 'sup': 6', '9, 'wherein an amount of the sp. strain for inoculating is 10to 10cfu/g.'}2. The method of preparation of claim 1 , wherein the corn gluten to which the moisture is added is heat-treated at 50 to 120° C. for 5 to 30 minutes after addition of the moisture.3BacillusBacillus. The method of preparation of claim 1 , wherein the sp. strain is an apathogenic sp. strain.4BacillusBacillus subtilis, Bacillus licheniformis, B. toyoi, B. coagulansB. polyfermenticus.. The method of preparation of claim 4 , wherein the apathogenic sp. strain is selected from the group consisting of claim 4 , and5. The method of preparation of claim 1 , wherein the solid-culturing is performed at a temperature of 30 to 45° C.6. Fermented corn gluten prepared by the method defined in claim 1 , comprising a low molecular weight protein.7. A feed additive comprising the fermented corn gluten defined in . This application is a ...

Filamentous fungal biomats, methods of their production and methods of their use

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

BACILLUS SP. STRAIN WITH IMPORIVED PRODUCTIVITY OF FERMENTED SOYBEAN MEAL AND METHOD FOR PRODUCIN FERMENTED SOYBEAN MEAL USING THE SAME

The present invention relates to a K2G strain, which is excellent in removal of anti-nutritional factors and in protease activity, and shows excellent antimicrobial activity against pathogens and reduced productivity of viscous substances, a method for producing a fermented soybean meal using the strain, a fermented soybean meal produced therefrom, and a feed composition including the same. The fermented soybean meal prepared by K2G strain according to the present invention has few anti-nutritional factors such as trypsin inhibitors, soybean oligosaccharides, and polysaccharides, a high content of crude proteins, and high protein solubility, and also consists of small-sized peptides digestible by livestock due to low-molecularization, thereby being effectively used as a high-quality vegetable protein feed having excellent absorption rate and feed efficiency. 1Bacillus amyloliquefaciens. A method for producing a fermented soybean meal by solid fermentation , comprising inoculating strain with Accession No. KCCM11471P into a soybean meal.2. The method according to claim 1 , comprising the steps of:a) adding water to a soybean meal to perform heat-treatment;{'i': 'Bacillus amyloliquefaciens', 'b) cooling the heat-treated soybean meal and inoculating the K2G strain therein; and'}{'i': 'Bacillus amyloliquefaciens', 'c) obtaining a fermented soybean meal by solid culture of the K2G strain inoculated in the soybean meal.'}3. The method according to claim 2 , wherein the water-added soybean meal in Step a) has a water content of 30 to 80%(v/w).4. The method according to claim 2 , wherein the heat treatment of Step a) is carried out by heating soybean meal at a temperature of 70 to 130° C. for 10 to 30 minutes.5. The method according to claim 2 , wherein the soybean meal in Step b) is cooled to a temperature of 30 to 50° C.6Bacillus amyloliquefaciens. The method according to claim 2 , wherein the number of K2G strain immediately after inoculation in Step b) is in a range of ...

BACILLUS MICROBIAL TERROIR FOR PATHOGEN CONTROL IN SWINE

Disclosed are methods of administering one or more strains to swine. The strains that are administered include 747 (NRRL B-67257), 1104 (NRRL B-67258), 1541 (NRRL B-67260), 1781 (NRRL B-67259), 2018 (NRRL B-67261), and BS1999 (NRRL B-67318). The strains improve bacterial homeostasis in the gastrointestinal tract by inhibiting bacterial pathogens such as and Administering the strains also improves performance such as weight gain and feed conversion. Useful combinations of strains and methods of using one or more strains are also provided. 1BacillusEscherichia coli, Salmonella, Clostridia,Streptococcus suis. A direct fed microbial composition comprising an isolated strain wherein the composition inhibits at least one pathogen selected from and in a gastrointestinal tract of a swine having ingested an effective amount of said direct fed microbial composition.2BacillusBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilis. The composition of claim 1 , wherein the isolated strain is chosen from at least one of strains 747 claim 1 , 1104 claim 1 , 1541 claim 1 , 1781 claim 1 , 2018 claim 1 , and 1999.3BacillusBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilisBacillus subtilis. The composition of claim 2 , wherein the composition comprises a combination of at least two isolated strains chosen from strains 747 claim 2 , 1104 claim 2 , 1541 claim 2 , 1781 claim 2 , 2018 claim 2 , and 1999.4. The composition of claim 1 , further comprising a carrier.5. The composition of claim 1 , further comprising a preservative.6BacillusBacillusBacillus. The composition of claim 1 , further comprising a cryoprotectant disposed about the isolated strain claim 1 , and wherein said isolated strain is a powdered lyophilized isolated strain.7BacillusBacillus. The composition of claim 6 , wherein the powdered lyophilized isolated strain comprises spores.8. The composition of claim 1 , further comprising an animal ...

METHODS FOR THE BIOSYNTHESIS OF TAURINE OR HYPOTAURINE IN CELLS

The present invention describes an approach to increase taurine or hypotaurine production in prokaryotes. More particularly, the invention relates to genetic transformation of organisms with genes that encode proteins that catalyze the conversion of cysteine to taurine, methionine to taurine, cysteamine to taurine, or alanine to taurine. The invention describes methods for the use of polynucleotides that encode cysteine dioxygenase (CDO) and sulfinoalanine decarboxylase (SAD) polypeptides in prokaryotes to increase taurine, hypotaurine or taurine precursor production. The preferred embodiment of the invention is in plants but other organisms may be used. Increased taurine production in prokaryotes could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed. 1. A cell comprising an expression cassette stably incorporated in its genome , the expression cassette comprising a promoter operably linked to an exogenous polynucleotide that is operably linked to a terminator , wherein the exogenous polynucleotide encodes a sulfinoalanine decarboxylase (SAD).2. The cell of claim 1 , wherein the SAD is encoded by a polynucleotide comprising a nucleotide sequence selected from the group consisting of the nucleotide sequence set forth in SEQ ID NO:5 and the nucleotide sequence set forth in SEQ ID NO:6.3. The cell of claim 1 , wherein the SAD comprises an amino acid sequence selected from the group consisting of the amino acid sequence set forth in SEQ ID NO: 7 and the amino acid sequence set forth in SEQ ID NO:8.4. The cell of claim 1 , wherein the promoter is a constitutive promoter or a non-constitutive promoter selected from the group consisting of a tissue-preferred promoter claim 1 , a tissue-specific promoter claim 1 , a cell type-specific promoter claim 1 , an inducible promoter claim 1 , a plant glutamate decarboxylase (GAD) promoter claim 1 , a plant sulphate transporter (SULTR) promoter claim 1 , and a plant glutamate ...

FEED COMPOSITION USING PERILLA MEAL AND METHOD FOR PRODUCING THEREOF

The present invention relates to a feed composition using perilla meal, in which the ratio of omega-3 (omega-3) fatty acid and omega-6 (omega-6) fatty acid is adjusted to 1:4 or less to obtain livestock products, such as meat or milk, containing a large amount of unsaturated fatty acid. More specifically, the present invention related to a feed composition using perilla meal to produce livestock products with the ratio of omega-3 to omega-6 fatty acids being 1:4 or less which is a recommended ratio of the World Health Organization (WHO). 1. A feed composition using perilla meal comprising:for 100 parts by weight of a feed composition, 20 to 40 parts by weight of roughage containing at least one of oats or rice straw; 6 to 8 parts by weight of shredded perilla meal having a diameter of 1.5 to 2.5 mm; 20 to 55 parts by weight of one or more additional ingredients selected from the group consisting of grain, salt, mineral, and calcium carbonate; and 0.5 to 1.5 parts by weight of probiotics;wherein the perilla meal is shredded so as to have a set size of 1.5 to 2.5 mm in diameter such that a ratio of omega-3 fatty acid and omega-6 fatty acid in at least one livestock product of meat or milk obtained from livestock is 1:4 or less,wherein at least any one of sugar, salt, or molasses is added to the perilla meal, and the perilla meal is stirred and simultaneously fermented for 50 to 72 hours at 45 to 55 ° C. in the presence of lactobacillus bacteria to prevent acidification.2. A method for preparing a feed composition using perilla meal comprising:1) preparing 20 to 40 parts by weight of roughage containing at least one of oats or rice straw;2) preparing 6 to 8 parts by weight of perilla meal, the perilla meal shredded to have a diameter of 1.5 to 2.5 mm, wherein at least one of sugar, salt, or molasses is added thereto, and the perilla meal is stirred and simultaneously fermented for 50 to 72 hours at 45 to 55 ° C. in the presence of lactobacillus bacteria;3) preparing 20 ...

Apparatus for producing fermented soybean meal

An exemplary embodiment of the present invention provides an apparatus for producing fermented soybean meal, which produces fermented soybean meal for monogastric animals and ruminant selectively or together. An apparatus for producing fermented soybean meal according to an exemplary embodiment of the present invention includes: a solid-liquid separating part, which mixes raw material soybean meal and an extraction solvent and extracts the soybean meal, and separately produces a remaining soybean meal and a soybean meal extract; a lactic acid bacteria culturing part, which produces the lactic acid bacteria by putting inoculum into the soybean meal extract, and supplies the lactic acid bacteria to the solid-liquid separating part; a solid substrate fermenting part, which is selectively supplied with and mixes at least two of the remaining soybean meal supplied from the solid-liquid separating part, the raw material soybean meal supplied through bypass, and lactic acid bacteria supplied from the lactic acid bacteria culturing part to produce a mixed material, and solid-substrate ferments the mixed material to produce primary solid substrate fermented soybean meal; and a drier, which dries the primary solid substrate fermented soybean meal supplied from the solid substrate fermenting part to produce secondary solid substrate fermented soybean meal.

ANIMAL FEEDS CONTAINING SPECIFIC GLYCOLIPIDS

Use of a sophorolipid in the preparation of a feed supplement for the improvement of feed efficiency in an animal. A feed supplement comprising a sophorolipid and lysolecithin mix is also provided for improving the feed conversion rate of an animal. 3. An animal feed as claimed in wherein Ris a hydrogen or a methyl group.4. An animal feed as claimed in claim 1 , wherein Ris a hydrocarbon group containing 16 to 18 carbon atoms claim 1 , more preferably 16 carbon atoms.5. An animal feed as claimed in wherein Rhas one site that is unsaturated (C═C bond).6. An animal feed as claimed in wherein the sophorolipids in the animal feed comprise at least 50% by weight sophorolipids having the general formula (2).7. An animal feed as claimed in wherein the sophorolipid comprises at least 0.05 wt. % to 0.1 wt. % of the total feed.8. An animal feed as claimed in further comprising at least one lysolecithin mixed with the sophorolipid.11. An ingredient claim 9 , premix or supplement as claimed in in combination with at least one lysolecithin.12. An ingredient claim 11 , premix or supplement as claimed in wherein the lysolecithin is provided in at an amount of at least 50% by weight of the ingredient claim 11 , premix or supplement.13. An ingredient claim 11 , premix or supplement as claimed in claim 11 , wherein the lysolecithin is provided in at an amount of at least 75% by weight of the ingredient claim 11 , premix or supplement.14. A method for increasing the feed conversion rate in an animal comprising feeding a mammal claim 1 , bird or fish with the animal feed according to .15. (canceled)16. (canceled)17. A method for increasing the feed conversion rate in an animal comprising feeding a mammal claim 9 , bird or fish with the ingredient claim 9 , premix or supplement according to . This invention relates to specific glycolipids for providing improved animal feeds.It is known to supplement animal feeds with different additives to improve animal performance, for example adding ...

ANIMAL FEED

An animal feed product disclosed herein has a feed mixture disposed in a container, where the feed mixture has a plurality of animal feed particles and seed butter coating each of the animal feed particles. A removable cover is coupled to the container over the cavity to encase the feed mixture. A method disclosed herein relates to heating a seed butter to reduce its viscosity. Animal feed particles are translated through a spraying region and each of the animal feed particles are coated with the heated seed butter to form a seed mixture. The seed mixture is deposited in a container defining a cavity and the feed mixture is compressed in the cavity. Other embodiments are described. 1. An animal feed product comprising:a container defining a cavity; a plurality of animal feed particles comprising a plurality of seeds; and', 'seed butter coating each of the plurality of animal feed particles, wherein the seed butter is no more than 20% of the feed mixture by weight; and, 'a feed mixture disposed in the cavity, the feed mixture comprisinga removable cover coupled to the container over the cavity, wherein the removable cover and the container encase the feed mixture.2. The animal feed product of claim 1 , wherein the animal feed particles further comprises at least one in the group consisting of: a plurality of dehydrated invertebrates and a plurality of dehydrated insects.3. The animal feed product of claim 2 , wherein the plurality of dehydrated invertebrates comprises at least one in the group consisting of: mealworms and black soldier fly larvae.4. The animal feed product of claim 1 , wherein the plurality of seeds comprise at least one type of seed in the group consisting of: sunflower claim 1 , grain claim 1 , legume claim 1 , and grass.5. The animal feed product of claim 1 , wherein the seed butter comprises sunflower seed butter.6. The animal feed product of claim 1 , wherein the seed butter contains at least one type of seed in the group consisting of: peanuts ...

METHODS FOR THE BIOSYNTHESIS OF TAURINE OR HYPOTAURINE IN CELLS

The present invention describes an approach to increase taurine or hypotaurine production in prokaryotes. More particularly, the invention relates to genetic transformation of organisms with genes that encode proteins that catalyze the conversion of cysteine to taurine, methionine to taurine, cysteamine to taurine, or alanine to taurine. The invention describes methods for the use of polynucleotides that encode cysteine dioxygenase (CDO) and sulfinoalanine decarboxylase (SAD) polypeptides in prokaryotes to increase taurine, hypotaurine or taurine precursor production. The preferred embodiment of the invention is in plants but other organisms may be used. Increased taurine production in prokaryotes could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed. 1. A prokaryotic cell comprising a first exogenous DNA which comprises two separate expression cassettes , wherein a first expression cassette comprises a first promoter operably linked to a first polynucleotide and a second expression cassette comprises a second promoter operably linked to a second polynucleotide , wherein the first polynucleotide encodes cysteine dioxygenase (CDO); and the second polynucleotide encodes sulfinoalanine decarboxylase (SAD) , wherein the polynucleotide encoding CDO encodes a protein having at least 27% identity to SEQ ID NO:3 or at least 25% identity to SEQ ID NO:4 each of which has CDO activity and wherein the polynucleotide encoding SAD encodes a protein having at least 29% identity to SEQ ID NO:7 or at least 30% identity to SEQ ID NO:8 each of which has SAD activity.2. A prokaryotic cell comprising a first exogenous DNA which comprises a single expression cassette , wherein the single expression cassette comprises a promoter operably linked to a polynucleotide which encodes cysteine dioxygenase (CDO) and encodes sulfinoalanine decarboxylase (SAD).3. The prokaryotic cell of claim 1 , wherein:the CDO comprises an amino acid sequence ...

NEW FERMENTATION MEDIUM FOR GROWTH OF METHANOTROPHIC BACTERIA AND METHOD FOR PRODUCING SAID MEDIUM

The present invention relates to a method for providing a fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism, the method comprises the step of: (i) providing a main growth solution; (ii) optionally sterilizing the main growth solution provided in step (i); (iii) providing a trace metal solution; and (iv) mixing the main growth solution (as provided in step (i) or (ii)) with the trace metal solution provided in step (iii) and providing the fermentation medium for the cultivation of at least one methanotrophic organism, or for the cultivation of a combination of organisms comprising at least one methanotrophic organism. 117-. (canceled)19. The method according to claim 18 , wherein the at least one precipitating trace metal compound is a salt of the at least one precipitating trace metal compound20. The method according to claim 18 , wherein the at least one precipitating trace metal compound is an iron compound claim 18 ,21. The method according to claim 18 , wherein the at least one precipitating trace metal compound or the Fe-compound is selected from Fe-compounds or Fe-compounds claim 18 , such as FeCl.6HO or FeSO4.7HO.22. The method according to claim 18 , wherein the acidic dissolved composition and/or the trace metal solution has a pH of pH 5.0 or below claim 18 , such as a pH of pH 4.5 or below claim 18 , e.g. a pH 4.0 or below claim 18 , such as a pH of pH 3.5 or below claim 18 , e.g. a pH 3.0 or below claim 18 , such as a pH of pH 2.5 or below claim 18 , e.g. a pH 2.0 or below claim 18 , such as a pH of pH 1.5 or below claim 18 , e.g. a pH 1.0 or below claim 18 , such as a pH of pH 0.5 or below claim 18 , e.g. a pH on the range of pH 0.5-5.0 claim 18 , such as in the range of pH 0.75-3 claim 18 , e.g. a pH on the range of pH 1.0-2.0 claim 18 , such as in the range of pH 1.25-1.5.23. The method according to claim 18 , wherein the ...

Cell with reduced ppgppase activity

The present invention relates to a cell which is genetically modified over its wild type in such a way that it has a reduced ppGppase activity relative to its wild type, and preferably an essential amino acid, even more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, to a feed additive comprising such a cell, to a method of preparing a cell overproducing essential amino acid, more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, comprising preparing a cell having a knocked-out gene coding for a ppGppase, and to a method of preparing an essential amino acid, more preferably an essential amino acid derived from serine, most preferably methionine or tryptophan, comprising the steps of a) culturing the cell according to the first aspect of the present invention or to any of its embodiments, and b) optionally: purifying the amino acid.

METHOD FOR FOODBORNE PATHOGEN REDUCTION IN POULTRY

A method for introducing a fermented product to poultry to reduce foodborne pathogens. The fermented product contains functional metabolites produced by and is added at a particular ratio to the feed for the most effective results. 113-. (canceled)14. A method for improving weight gain in poultry which have been administered a coccidia vaccine , comprising the steps of:supplementing a feed with a fermentation product derived from a yeast to produce a total feed,{'i': 'Saccharomyces cerevisiae', 'wherein the fermentation product is derived at least partially from ; and'}wherein the fermentation product is added to the feed such that the fermentation product is between 0.004% and 0.2% of the weight of the total feed,and feeding the total feed to poultry, wherein the poultry have been administered a coccidia vaccination prior to feeding,wherein the body weight of the poultry after 16 days is greater than the body weight of control poultry which were administered a coccidia vaccine but not fed a total feed including the fermentation product.15. The method of claim 14 , wherein:{'i': 'Saccharomyces cerevisiae', 'the fermentation product comprises functional metabolites produced by a yeast.'}16. The method of claim 14 , wherein:the fermentation product has a crude protein content, a crude fat content, and a crude fiber content; and comprisesamino acids,potassium, phosphorous and calcium.1718-. (canceled)19. The method of claim 16 , wherein the crude protein content is a minimum of 12.0%.20. The method of claim 16 , wherein the crude fat content is a minimum of 1.2%.21. The method of claim 16 , wherein the crude fiber content is a maximum of 30.0%.22. The method of claim 16 , wherein the ash content of the fermentation product is a maximum of 10.8%.23. The method of claim 14 , wherein the amount of fermentation product added to the feed is about 0.125% of the total feed.24. The method of claim 14 , wherein the poultry is not administered an antibiotic after coccidia ...

PROCESSES AND SYSTEMS FOR METABOLITE PRODUCTION USING HYDROGEN RICH C1-CONTAINING SUBSTRATES

The invention is directed to a process for producing one or more fermentation product in a multi-stage process including an inoculation reactor and at least one bioreactor. The inoculation reactor is fed a C1-containing gaseous substrate containing a reduced amount of hydrogen. The hydrogen is reduced to increase the proportion of CO in the C1-containing gaseous substrate being provided to the inoculation reactor. The inoculation reactor ferments the CO-rich C1-containing gaseous substrate and produces an inoculum, which is fed to at least one bioreactor. The bioreactor receives the C1-containing gaseous substrate, which may or may not contain reduced amounts of hydrogen, to produce one or more fermentation product. By providing a CO-rich C1-containing gaseous substrate to the inoculation reactor, both the inoculation reactor and the subsequent bioreactor(s), are able to have increased stability and product selectivity. 1. A process for producing one or more fermentation product , the process comprising;a. providing a CO-rich C1-containing gaseous substrate to an inoculation reactor comprising a liquid nutrient medium containing a culture of one or more C1-fixing microorganism;b. fermenting the CO-rich C1-containing gaseous substrate to produce an inoculum;c. passing at least a portion of the inoculum to a bioreactor system, the bioreactor system comprising at least one bioreactor containing a culture of one or more C1-fixing microorganism in a liquid nutrient medium;{'sub': '2', 'd. passing an Hrich C1-containing gaseous substrate to the bioreactor system; and'}{'sub': '2', 'e. fermenting the Hrich C1-containing gaseous substrate to produce at least one fermentation product.'}2. The process of claim 1 , wherein the CO-rich C1-containing gaseous substrate comprises Hat an H:CO molar ratio of less than 1:1.3. The process of claim 1 , wherein the CO-rich C1-containing gaseous substrate comprises Hat an H:CO molar ratio of less than 0.5:1.4. The process of claim 1 , ...

GAS FERMENTATION FOR THE PRODUCTION OF PROTEIN OR FEED

The invention provides animal feed comprising microbial biomass and methods of producing animal feed by culturing a microorganism to produce microbial biomass. In particular, the invention relates to animal feed produced by fermentation of a gaseous substrate comprising one or more of CO, CO, and H, especially by a Gram-positive, anaerobic, and/or microorganism. 1. A process comprising:{'sub': 2', '2, 'a. obtaining a gaseous substrate comprising one or more of CO, or CO, and Hfrom an industrial process waste gas, an industrial process off gas, or syngas;'}{'i': 'Acetobacterium woodii, Alkalibaculum bacchii, Blautia producta, Butyribacterium methylotrophicum, Clostridium aceticum, Clostridium autoethanogenum, Clostridium carboxidivorans, Clostridium coskatii, Clostridium drakei, Clostridium formicoaceticum, Clostridium ljungdahlii, Clostridium magnum, Clostridium ragsdalei, Clostridium scatologenes, Eubacterium limosum, Moorella thermautotrophica, Moorella thermoacetica, Oxobacter pfennigii, Sporomusa ovata, Sporomusa silvacetica, Sporomusa sphaeroides, Thermoanaerobacter kivui', 'b. introducing the gaseous substrate to a vessel and contacting with a microorganism, which is an anaerobe selected from , and mixtures thereof, under fermentation conditions including a dilution rate greater than 1/day, to maximize production of microbial biomass, and to generate a product selected from acetate, acetic acid, ethanol, 2,3-butanediol, or lactic acid, in a fermentation broth;'}c. isolating the biomass from the fermentation broth,d. drying the microbial biomass wherein the microbial biomass contains greater than 85% protein by weight on a dry solids basis, and combining the microbial biomass with an excipient to produce animal feed,e. separating at least one product from at least a portion of the fermentation broth.2. The process of wherein the syngas is generated through gasification of coal claim 1 , refinery residues claim 1 , biomass claim 1 , lignocellulosic material ...

Enhanced aerobic fermentation methods for producing edible fungal mycelium blended meats and meat analogue compositions

Provided herein are shelf-stable protein food ingredients, food products comprising the shelf-stable protein food ingredients, methods of their production, and methods of their use. The shelf-stable protein food ingredients comprise cultured fungal biomass and a limited amount of water. Advantageously, the shelf-stable protein food ingredients can be stored, transported, and delivered within the food supply.

Method for Preparing Feed by Bacteria-enzyme Synergistic Fermentation

The disclosure discloses a method for preparing feed by bacteria-enzyme synergistic fermentation, belonging to the technical field of fermentation engineering. According to the disclosure, JUN-DY-6, protease and cellulase are used as a starter, and seed meal or rapeseed meal is used as a substrate. The bacteria-enzyme fermentation product has higher yield of organic acids and flavor substances and better palatability, and can be used for preparing feed additives. The of the disclosure can inhibit growth of harmful bacteria such as and in the intestinal tract of poultry and livestock, and is good for health of the intestinal tract. The method increases the added value of seed meal and rapeseed meal, and is conductive to reuse of waste. 1Lactobacillus plantarumL. plantarumCamellia. A method for preparing feed , comprising using () JUN-DY-6 and enzymes to co-treat a raw material; wherein the raw material is rapeseed meal or seed meal; the enzymes comprise protease and cellulase; a moisture content of the raw material is 30-50% , and a content of the cellulase is 300-400 U/g substrate; the protease is neutral protease or alkaline protease;a content of the alkaline protease is 800-1200 U/g substrate; anda content of the neutral protease is 1350-1500 U/g substrate.2L. plantarum. The method according to claim 1 , wherein the collection number of the JUN-DY-6 is CCTCC NO: M 2017138.3L. plantarumCamelliaL. plantarum. The method according to claim 2 , wherein the JUN-DY-6 claim 2 , the alkaline protease and the cellulase are added to an environment containing seed meal and then fermentation is carried out; wherein the moisture content is 30-50% claim 2 , the protease is the alkaline protease claim 2 , the content of the alkaline protease is 800-1500 U/g substrate claim 2 , and an inoculum size of the JUN-DY-6 is 1-5%.4. The method according to claim 3 , wherein the fermentation is fermentation at 35° C.-37° C. for at least 20 h.5L. plantarum. The method according to claim 4 , ...

METHODS FOR UPGRADING SPENT BIOMASS MATERIAL

Disclosed is a fibrous solid substrate in pellet form suitable for use in agriculture. The fibrous solid substrate in pellet form is obtained from spent biomass material following an anaerobic fermentation and biogas production. Further disclosed is a method for cultivating fungal cells and/or spores using said fibrous solid substrate in pellet form. Also disclosed are methods for upgrading spent biomass material from a biogas production unit to fertilizer products, fibers and substrate for biogas. 1. A fibrous solid substrate in pellet form obtained by a method comprising the steps ofa. providing a biomass material comprising solid and liquid parts from a biogas fermenter following an anaerobic fermentation and biogas production, i) a fibrous solid fraction comprising organic and inorganic nitrogen parts and having a reduced content (w/w) of water, and comprising one or more macromolecular nutrient constituents selected from the group consisting of cellulose, hemicellulose, lignin and lignocellulose, and', 'ii) at least one liquid fraction comprising solid and liquid organic and inorganic phosphor-containing parts,, 'b. subjecting the fermented biomass material of step a. to one or more separation steps resulting in the provision of'} comprising the steps of i) heating the fibrous solid fraction to a temperature of more than 70° C., optionally under alkaline pH conditions, and optionally ii) subjecting the fibrous solid fraction comprising organic and inorganic nitrogen parts to a pressure of more than 1 bar; and/or not comprising addition of non-fermented biomass and water to the fibrous solid fraction (i.e composting),', 'wherein said treatment i) reduces or eliminates viable microorganisms present in the fibrous solid fraction, and/or ii) reduces the contents of volatile nitrogen-containing compounds and/or precursor volatile compounds present in the fibrous solid fraction, and, 'c. subjecting the fibrous solid fraction of step b. to a sanitation treatment, said ...

PROCESS FOR MANUFACTURING AN ALKALI GRANULATED ADDITIVE FOR ANIMAL FEED AND ALKALI GRANULATED ADDITIVE FOR ANIMAL FEED

The invention refers to an alkali granulated additive for animal feed with high concentration of lysine, but especially low ammonium content. More particularly, said alkali granulated additive has pH within the range between 8 and 10, including a lysine concentration between 70% and 85% by weight and having a nearly negligible content of ammonium ions which the molar relation follows the condition below: 18-. (canceled)9. A method comprising: monitoring the nitrogen source by measuring a concentration of ammoniacal nitrogen within the culture medium,', 'maintaining the concentration of ammoniacal nitrogen at about 100 mg of ammoniacal nitrogen per liter of the culture medium by introducing, in response to the concentration of ammoniacal nitrogen approaching a predetermined level, a solution to the culture medium, the solution being configured to increase the concentration of ammoniacal nitrogen to about 100 mg of ammoniacal nitrogen per liter of the culture medium, and', 'controlling reduction of the concentration of ammoniacal nitrogen to between 50 mg of ammoniacal nitrogen per liter of the culture medium and 10 mg of ammoniacal nitrogen per liter of culture medium until an end of the carbon source to thereby produce a fermented broth; and, 'fermenting, inside a fermentation tank, a culture medium including a carbon source and a nitrogen source to produce lysine, the fermentation including lysine in a first amount between 70% and 85% by weight on a basis of a weight of the alkali granulated additive, and', {'sup': '−2', 'ammonia in a second amount being less than 10mols of ammonia per mol of lysine.'}], 'producing, from the fermented broth, an alkali granulated additive for animal feed having a pH between 8 and 10, the alkali granulated additive including10. The method of claim 9 , wherein the solution is an ammonium sulfate solution with a concentration between 200 g ammonium sulfate per liter of solution and 450 g ammonium sulfate per liter of solution claim 9 , ...

PROCESS FOR PRODUCING HIGH PROTEIN BIOMASS FROM STARCH-CONTAINING CEREAL MATERIALS BY YEAST STRAINS

A process of fermentation of starch-containing cereal materials, such as a process of producing high protein biomass from starch-containing cereal materials by yeast strains, includes following steps: producing glucose for use as a substrate from starch-containing cereal materials by liquefaction and saccharification; selecting yeast strains which produce high protein biomass and storing the selected yeast strains; and producing high protein biomass from yeast strains by using the glucose produced, optionally in combination with molasses. The produced high protein biomass is useful in animal husbandry, especially for pig, aquatic, poultry, and large-scale animal husbandry on an industrial scale. The produced high protein biomass can replace antibiotics in the livestock industry, thereby reducing imports of products from abroad. 1. A process for producing high protein biomass from starch-containing cereal materials by yeast strains , comprising the following steps: cleaning the starch-containing cereal materials and grounding;', 'carrying out liquefaction by raising the temperature to 85° C. and adjusting the pH to 5.5, then adding the enzyme α-amylase to carry out the liquefaction within two hours;', 'performing saccharification by lowering the temperature to 65° C. after two hours of liquefaction, and adjusting the pH to 4.5, then quantifying the α-glucoseamylase enzyme immediately after cooling and adjusting the pH to 4.5, before the saccharification process takes place;', 'conducting glucose content analysis by qualitative test using reagents such as iodine to determine the end of the saccharification process; and', 'separating glucose from the residue and stores the resulting sugars;, 'i) producing glucose for use as a substrate from the starch-containing cereal materials by liquefaction and saccharification, including [{'i': S. cerevisiae, Pichia pastoris, C. utilis, Torulopsis, Geotrichum candidum, Kluyveromyces marxianus, Endomyces vernali, C. arbores, C. ...

USE OF HYDROTHERMALLY TREATED BIOMASS AS PATHOGEN BINDER

The present invention relates to microorganism biomass and a feed or food composition comprising said biomass for use in preventing or reducing the adverse effects of pathogens in animal or human digestive tract. 1. A method for preparing a feed additive , said method comprising the steps of(a) providing an aqueous suspension comprising microbial biomass,(b) subjecting said aqueous suspension comprising said microbial biomass to a temperature of at least 160° C. for is to 360 minutes at a pressure above 5 bar,(c) separating a solid phase containing the microbial biomass,(d) optionally, drying the microbial biomass obtained from step (c).2. The method according to claim 1 , wherein step (b) is conducted under conditions corresponding to a severity factor of at least 2.5 claim 1 , preferably of at least 3.5 claim 1 , most preferably at least 3.9.3. The method according to claim 1 , wherein step (b) is conducted under conditions corresponding to a severity factor in the range of of about 3.5 to about 5.5 such as e.g. about 4 to about 4.9 claim 1 , such as e.g. about 4.1 to about 4.8.4. The method according to claim 1 , wherein step (b) is conducted during a period of e.g. about 5 minutes and up to about 120 minutes claim 1 , such as e.g. about 10 minutes and up to about 60 minutes claim 1 , while the temperature is about 160° C. and up to about 220° C. claim 1 , such as e.g. about 180° C. and up to about 220° C.5. The method according to claim 1 , wherein said aqueous suspension comprising microbial biomass contains cultivation medium comprising lignocellulose hydrolysate.6. The method according to claim 1 , wherein the microbial biomass obtained in step (c) further comprises residues of lignocellulose.7. The method of according to claim 1 , wherein the microbial biomass is obtained from oleaginous microorganisms.8. The method of according to claim 5 , wherein the microbial biomass of step (c) is obtained by subjecting the oleaginous microorganisms to a step of ...

SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELL

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. 1221-. (canceled)222. A process for producing fish comprising:{'i': 'Brassica', 'claim-text': i) a Δ6 desaturase, a Δ6 elongase and a Δ5 desaturase; or', 'ii) a Δ5/Δ6 bifunctional desaturase and a Δ5/Δ6 bifunctional elongase;', {'i': 'Brassica', 'wherein the heterologous polynucleotides are operably linked to one or more promoters that are capable of directing expression of said polynucleotides in seed cells, wherein the enzymes encoded by said polynucleotides comprise at least one desaturase which is able to act on an acyl-CoA substrate; and'}], 'a) obtaining feedstuff which comprises oil extracted from transgenic seeds which comprise eicosapentaenoic acid (EPA) and heterologous polynucleotides which encode enzymes that compriseb) administering the feedstuff to fish,thereby producing the fish.223Brassica. The process of claim 222 , wherein the enzymes encoded by the polynucleotides comprise at least one Δ5 elongase which catalyses the conversion of EPA to docosapentaenoic acid (DPA) in the seed cells.224. The process of claim 222 , wherein at least one promoter is a seed specific promoter.225Brassica. The process of claim 222 , wherein the seeds comprise docosapentaenoic (DPA).226Brassica. The process of claim 222 , wherein the seeds comprise docosahexaenoic acid (DHA).227Brassica. The process of claim 222 , wherein the seeds comprisea) DHA which is produced from a fatty acid which is ALA, LA, GLA, arachidonic acid (ARA), SDA, ETA, EPA, DPA, or ...

METHOD FOR CONCENTRATING PROTEIN IN GRAIN POWDER

Provided are a method of concentrating protein in grain powder, grain powder including protein that has been concentrated by using the method, and a feed additive including the grain powder including concentrated protein. According to the method of concentrating protein in grain powder, grain powder is treated with enzyme to increase the water-soluble saccharide content in a source, and by inoculating bacteria or yeast and fermentation, the increased water-soluble saccharide is removed, leading to a higher concentration of protein. Thus, the protein content ratio increase effects and the function of grain powder as a protein source are enhanced. 1. A method of concentrating protein in grain powder , the method comprising:treating grain powder with enzyme to decompose structural carbohydrate; andinoculating yeast into the grain powder to perform fermentation.2Saccharomyces.. The method of claim 1 , wherein the yeast is genus3. The method of claim 1 , wherein the grain powder is corn gluten.4. The method of claim 1 , wherein the fermentation is solid-state fermentation.5. The method of claim 1 , wherein the structural carbohydrate comprises at least one selected from the group consisting of starch claim 1 , cellulose claim 1 , hemicellulose claim 1 , or pectin.6. The method of claim 1 , wherein the enzyme is α-amylase or glucoamylase.7. The method of claim 1 , wherein the enzyme is used in an amount of 0.1 to 1 part by weight based on 100 parts by weight of the grain powder. This application is a Divisional of copending application Ser. No. 15/741,447, filed on Jan. 2, 2018, which was filed as PCT International Application No. PCT/KR2016/010705 on Sep. 23, 2016, which claims the benefit under 35 U.S.C. § 119(a) to Patent Application No. 10-2015-013660, filed in Korea on Sep. 25, 2015, and Patent Application No. 10-2016-0031463, filed in Korea on Mar. 16, 2016 all of which are hereby expressly incorporated by reference into the present application.The present ...

SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELLS

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. 1. A process for producing oil , comprising extracting oil from a recombinant cell which is capable of synthesising a long chain polyunsaturated fatty acid(s) (LC-PUFA) , which comprises one or more polynucleotides which encode at least two enzymes each of which is a Δ5/Δ6 bifunctional desaturase , Δ5 desaturase , Δ6 desaturase , Δ5/Δ6 bifunctional elongase , Δ5 elongase , Δ6 elongase , Δ4 desaturase , Δ9 elongase , or Δ8 desaturase , wherein the one or more polynucleotides are operably linked to one or more promoters that are capable of directing expression of said polynucleotides in the cell , wherein said recombinant cell is derived from a cell that is not capable of synthesising said LC-PUFA.2. (canceled)3. The process of claim 1 , wherein at least one of the enzymes in the recombinant cell is a Δ5 elongase.4. The process of claim 3 , wherein the Δ5 elongase also has Δ6 elongase activity claim 3 , and wherein the elongase is more efficient at synthesizing DPA from EPA than it is at synthesizing ETA from SDA.5. (canceled)6. (canceled)7. The process of claim 3 , wherein the Δ5 elongase can be purified from algae.814-. (canceled)15. The process of claim 1 , wherein at least one of the enzymes in the recombinant cell is a Δ5/Δ6 bifunctional desaturase or a Δ5/Δ6 bifunctional elongase.1618-. (canceled)19. The process of claim 1 , wherein at least one of the enzymes in the recombinant cell is a Δ5 desaturase.20. The process of claim 1 , wherein at ...

METHOD FOR PREPARING FEEDSTUFFS COMPRISING BUTYRIC ACID AND/OR BUTYRATE

A method for preparing a feedstuff comprising butyric acid and/or butyrate, comprising: adding a microorganism into a light corn steepwater to provide a mixture, wherein the microorganism comprises a first strain and the first strain is able to metabolize saccharides and/or organic compounds in a fermentation to produce butyric acid; keeping the mixture under an anaerobic atmosphere to conduct the fermentation to provide a fermentation broth; and optionally condensing the fermentation broth. Optionally, the microorganism further comprises a second strain, wherein the second strain is able to fix a carbon oxide. 1. A method for preparing a feedstuff comprising butyric acid and/or butyrate , comprising:adding a microorganism into a light corn steepwater to provide a mixture, wherein the microorganism comprises a first strain and the first strain is able to metabolize saccharides and/or organic compounds in a fermentation to produce butyric acid;keeping the mixture under an anaerobic atmosphere to conduct the fermentation to provide a fermentation broth; andoptionally condensing the fermentation broth.2ClostridiumButyribacteriumButyrivibrio. The method as claimed in claim 1 , wherein the first strain is at least one of sp. strain claim 1 , sp. strain claim 1 , and sp. strain.3Clostridium tyrobutyricum, Clostridium butyricum, Clostridium beijerinckii, Clostridium acetobutylicum, Clostridium argentinense, Clostridium aurantibutyricum, Clostridium botulinum, Clostridium carboxidivorans, Clostridium cellulovorans, Clostridiumsaccharolyticum, Clostridium difficile, Clostridium kluyveri, Clostridium novyi, Clostridium paraputrificum, Clostridium pascui, Clostridium pasteurianum, Clostridium peptidivorans, Clostridium perfringens, Clostridium scatologenes, Clostridium schirmacherense, Clostridium sticklandii, Clostridium subterminaleClostridium symbiosum, Clostridium tetani, Clostridium tepidiprofundi, Clostridium tertium, Clostridium tetanomorphumClostridium thermopalmarium ...

SYSTEMS AND METHODS FOR PRODUCING FERMENTED MILK REPLACER AND METHODS OF FEEDING SAME TO ANIMALS

Young animals such as calves may be fed milk replacers containing soy having been preserved through treatment with acid-producing bacteria that reduces a pH of the milk replacer through microbial digestion of sugars present in soy to thereby produce a fermented milk replacer. The milk replacer is edible for at least 48 hours and its digestibility in the young animals is increased due to the microbial digestion of sugars in the soy that are otherwise indigestible in the gut of the young animals. The fermented milk replacers may be ingested by young animals in group settings. In addition, the milk replacers containing soy may be treated with enzymes adapted to hydrolyze complex indigestible carbohydrates within the milk replacer, potassium sorbate or both. Feeding systems incorporating these fermented milk replacers may result in increased gain and increased dry matter intake without negatively affecting animal health. 1. A method of feeding a young animal , comprising:in a tank or feeder, fermenting a liquid milk replacer comprising soy by treating the liquid milk replacer with an acid-producing bacteria; andfeeding the young animal the fermenting liquid milk replacer.2. The method of claim 1 , wherein treating the liquid milk replacer with an acid-producing bacteria comprises adding a starter culture of the acid-producing bacteria to the liquid milk replacer in the tank or feeder.3. The method of claim 1 , wherein treating the liquid milk replacer with an acid-producing bacteria comprises adding a starter culture of the acid-producing bacteria to the liquid milk replacer prior to adding the liquid milk replacer to the tank or feeder.4. The method of claim 1 , wherein the fermenting liquid milk replacer has a pH of about 4.0 to about 6.0 when fed to the young animal.5. The method of claim 1 , wherein the liquid milk replacer comprises potassium sorbate.6. The method of claim 5 , further comprising activating the potassium sorbate by reducing the pH of the liquid milk ...

NUTRITIVE COMPOSITIONS AND METHODS RELATED THERETO

Nutritive compositions containing an organism having attenuated or no poly(3-hydroxybutyrate) production are provided. These nutritive compositions are useful as feed, such as animal feed and feed additives for ruminants, non-ruminants and aquaculture, etc. Materials and methods for production of these organisms are provided. 1Cupriavidus necator.. A nutritive composition comprising an organism originally capable of producing poly(3-hydroxybutyrate)(PHB) , wherein the organism has attenuated and/or no PHB production , wherein the organism does not comprise an exogenous nucleic acid , wherein the organism has at least one modification of one or more of the phaCAB operon enzymes excluding a phasin modification , and wherein the organism is2. The nutritive composition of , wherein the organism has a higher concentration of vitamin , minerals , iron , cholesterol , fat , lipid , carbohydrate , amino acid(s) , oligopeptides , polypeptides or derivatives thereof , as compared to an organism not modified in accordance with .3. The nutritive composition of , wherein the organism has higher crude protein content as compared to an organism not modified in accordance with .4. The nutritive composition of wherein the organism is obtained via genetic engineering claim 1 , by adaptive mutation claim 1 , or by selective isolation of naturally occurring mutant strains that do not produce PHB.5. The nutritive composition of claim 1 , wherein the PHB production in the organism is attenuated or eliminated prior to any fermentation of the organism.6. The nutritive composition of claim 1 , wherein the PHB production in the organism is attenuated or eliminated by elimination or attenuation of activity comprising one or more phaCAB operon enzymes excluding a phasin modification.7. The nutritive composition of claim 6 , wherein activity of one or more phaCAB operon enzymes is eliminated or attenuated by modifying a gene encoding a phaCAB operon enzyme by a gene deletion claim 6 , a gene ...

PROCESSING BIOMASS

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. 1. A method for processing biomass , the method comprising:combining a solid enzyme with a pretreated biomass.2. The method of claim 1 , wherein the method further comprises producing a product from the combination of the solid enzyme and the pretreated biomass.3. The method of claim 1 , further comprising feeding the combination to an animal.4. The method of claim 1 , wherein the method further comprises densifying the combination.5. The method of wherein the enzyme is selected from the group consisting of phytase claim 1 , cellulase claim 1 , lactase claim 1 , lipase claim 1 , pepsin claim 1 , catalase claim 1 , xylanase claim 1 , and pectinase.6. The method of claim 1 , wherein the enzyme comprises a cellulase.7. The method of claim 1 , wherein the biomass comprises a cellulosic or lignocellulosic material.8. The method of claim 1 , wherein the biomass is selected from the group consisting of paper claim 1 , paper products claim 1 , paper waste claim 1 , wood claim 1 , particle board claim 1 , sawdust claim 1 , agricultural waste claim 1 , sewage claim 1 , silage claim 1 , grasses claim 1 , rice hulls claim 1 , bagasse claim 1 , jute claim 1 , hemp claim 1 , flax claim 1 , bamboo claim 1 , sisal claim 1 , abaca claim 1 , straw claim 1 , corn cobs claim 1 , corn stover claim 1 , switchgrass claim 1 , alfalfa claim 1 , hay claim 1 , coconut hair claim 1 , cotton claim 1 , seaweed claim 1 , algae claim 1 , and mixtures thereof.9. The method of wherein the native biomass material includes a genetically modified plant material.10. The method of claim 2 , wherein the method comprises adding water to the combination and then sacharifying the combination to produce the product comprising sugars.11. The method of claim 1 , wherein the pretreated biomass is pretreated using one or more of size reduction claim ...

METHOD OF ISOLATING LIPIDS FROM A LYSED LIPIDS CONTAINING BIOMASS BY EMULSION INVERSION

The current invention relates to a method of separating lipids from a lipids containing biomass by demulsification under mild conditions, to an oil and to a delipidated biomass as obtained by such a method. 115-. (canceled)16. A method of isolating a lipid from a lipid containing biomass , comprising the following steps:a) providing an aqueous suspension of a biomass comprising cells which contain the lipid;b) optionally lysing the cells of the biomass;c) concentrating the suspension until the volume ratio between oil and water in the suspension exceeds the value of 1;d) separating the oil containing light phase from the aqueous phase.17. The method of claim 16 , wherein the suspension is concentrated to a total dry matter (TDM) content of more than 60 wt.-%.18. The method of claim 16 , wherein the suspension is concentrated to a total dry matter (TDM) content of of between 65 and 80 wt.-%.19. The method of claim 16 , wherein concentration of the suspension is carried out by evaporation of water at a temperature not higher than 100° C.20. The method of claim 16 , wherein concentration of the suspension is carried out by evaporation of water at a temperature of between 70° C. and 100° C.21. The method of claim 16 , wherein cells of the biomass are lysed enzymatically claim 16 , mechanically claim 16 , chemically and/or physically.22. The method of claim 16 , wherein the suspension is provided as a fermentation broth with a biomass density of at least 80 g/l.23. The method of claim 16 , wherein the suspension is provided as a fermentation broth and/or with a biomass density of 160 g/l to 200 g/l.24. The method of claim 16 , wherein the cells of the biomass comprise an average of at least 10 wt.-% of lipids.25. The method of claim 16 , wherein the cells of the biomass comprise an average of at least 40 wt.-% of lipids.26. The method of claim 16 , wherein the cells which contain lipids comprise PUFAs containing lipid and are selected from algae claim 16 , fungi claim 16 ...

Bee Bread Containing Astaxanthin and Hydrophilic Colloid and Preparation Method Thereof

A bee bread containing a base material of the bee bread, the hydrophilic colloid, ethanol and water, and is prepared according to a certain preparation method. The bee bread containing the astaxanthin and the hydrophilic colloid has the advantages of long preservation time and small nutrient loss. The bee which are fed with the bee bread for a long time can effectively increase the yield of royal jelly and the number of larvae, and extend the life. 1. A bee bread , comprising: a base material of the bee bread , astaxanthin , hydrophilic colloid , ethanol and water.2. The bee bread according to claim 1 , wherein claim 1 , the base material of the bee bread contains 50% of pollen and 50% of fermented soybean meal.3. (canceled)4. The bee bread according to claim 1 , wherein claim 1 , the hydrophilic colloid is xanthan gum claim 1 , gelatin claim 1 , carrageenan claim 1 , pectin claim 1 , arabic gum or guar gum.5. A method for preparing the bee bread of claim 1 , comprising following specific steps:S1: crushing a certain amount of base material of the bee bread as a standby;S2: weighing and taking a certain mass of astaxanthin, wherein a mass ratio of the base material of the bee bread to the astaxanthin is 100:0.2-0.6;S3: preparing the ethanol aqueous solution with a concentration of 50-100%;S4: uniformly mixing the astaxanthin with a certain mass of ethanol aqueous solution, to prepare the astaxanthin-ethanol solution;S5: uniformly mixing the astaxanthin-ethanol solution with the base material of the bee bread, to obtain a mixture of the astaxanthin and the base material of the bee bread;S6: preparing a hydrophilic colloid aqueous solution with a concentration of 0.05%-0.1%, adding the the hydrophilic colloid aqueous solution to the mixture of the astaxanthin and the base material of the bee bread in the step 5, and mixing uniformly; andS7: placing the mixture prepared in the step S6 at the temperature of 50° C. and drying, and then crushing, to obtain the bee bread.6 ...

Methods of processing waste activated sludge

Methods for processing waste activated sludge obtainable from wastewater plants. The methods herein are useful in that they provide novel waste activated sludge preparations that are substantially free of live microbial organisms and contain a high content of digestible protein.

Nutrient rich compositions

This invention relates to processes and systems for converting fresh food waste into nutrient rich hydrolysates and particulate compositions. The invention also relates to the hydrolysates and compositions useful, for example, as fertilizers, feedstock or other nutrient supplements.

EUGLENA DERIVED ANIMAL FEED COMPOSITION

An animal feed composition includes a feed component made from corn, soy, corn or soy derivatives or byproducts, or grains and an added whole cell biomass, including beta-1,3-glucan having at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers with an average molecular weight of about 1.2 to 580 kilodaltons (kDa). Beta-glucan polymer chains have a polymer length of about 7.0 to 3,400 glucose monomers. The whole cell biomass has at least 30 percent beta-1,3-glucan and residual media remaining from a heterotrophic fermentation process and is about 0.0001 to 0.0124 percent of the composition. It is possible to add a lysate. 1. An animal feed composition , comprising:a feed component comprising one or more of corn, soy, corn or soy derivatives or byproducts, or grains;{'i': Euglena', 'Euglena', 'Euglena', 'Euglena, 'an added whole cell biomass, including beta-1,3-glucan comprising at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of about 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers, wherein the whole cell biomass comprises at least 30 percent beta-1,3-glucan and includes residual media remaining from a heterotrophic fermentation process that produced the whole cell biomass, wherein the added whole cell biomass and residual media are about 0.0001 to 0.0124 percent w/w of the composition.'}2Euglena. The composition according to claim 1 , wherein the added whole cell biomass and residual media are about 0.001 to 0.01 percent w/w of the composition.3Echinaceaginsenggingko bilobaganoderma, astragaluspapaya. The composition according to claim 1 , where the composition further comprises an added immune response inducing component comprising one or more of vitamin C claim 1 , claim 1 , aloe claim 1 , golden seal claim 1 , claim 1 , garlic claim 1 , bell peppers claim 1 , ginger claim 1 , tumeric claim ...

EUGLENA DERIVED COMPOSITION HAVING IMMUNE RESPONSE INDUCING COMPONENTS

A composition includes a biomass lysate having an average particle size of about 2.0 to 500 micrometers and cellular components, including beta-1,3-glucan as at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers. Residual media remains from a heterotrophic fermentation process that produced a biomass and the lysate. Vitamin C is added in an example, and the weight ratio of the beta-1,3-glucan to the added vitamin C is in the ratio of 1:10 to 1:1 and may be in different delivery forms. The composition may include other added immune response inducing components. 1. A composition , comprising:{'i': Euglena', 'Euglena, 'a biomass lysate having an average particle size of about 2.0 to 500 micrometers and comprising cellular components, including beta-1,3-glucan comprising at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of about 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers, wherein the biomass lysate comprises at least 30 percent beta-1,3-glucan;'}{'i': Euglena', 'Euglena, 'residual media remaining from a heterotrophic fermentation process that produced a biomass and the lysate, and including an added immune response inducing component, and wherein the composition is in the form of a capsule, a tablet, a powder, a lotion, a gel, a stick pack, a liquid solution, a liquid suspension, a gummy, a multivitamin, a health shake, a health bar or a cookie.'}2Echinaceaginsenggingko bilobaganoderma, astragaluspapaya. The composition according to claim 1 , wherein said added immune response inducing component comprises one or more of vitamin C claim 1 , claim 1 , aloe claim 1 , golden seal claim 1 , claim 1 , garlic claim 1 , bell peppers claim 1 , ...

Fish feed, methods for preparing feed, and feeding fish

Fish feed, methods for preparing feed, and methods of providing feed to fish are described herein. A method of providing feed to fish may include providing a first feed composition to the fish prior to the fish growing to a weight of about 50% to about 90% of a desired body weight and providing a second feed composition to the fish after the fish grows to a weight of about 50% to about 90% of the desired body weight. The first feed composition may include a first concentration of at least one of eicosapentaenoic acid and docosahexaenoic acid. The second feed composition may include a second concentration of at least one of eicosapentaenoic acid and docosahexaenoic acid. The second concentration may be at least about 30% greater than the first concentration.

PROCESSES OF TREATING GRAIN

Processes of treating grain (e.g., corn), involving milling the grain to produce milled grain wherein the grain germ remains intact in the milled grain, and producing a mixture by mixing the milled grain with water and at least one enzyme selected from the group consisting of protease, alpha amylase, glucoamylase, cell wall degrading enzyme, and mixtures thereof, wherein the pH of the mixture is optionally adjusted to a pH of about 3.5 to about 6.5, and incubating the mixture for about 1 to about 3 hours to produce an incubated mixture. 1. A process of treating grain , said process comprising milling said grain to produce milled grain wherein the grain germ remains intact in said milled grain , and producing a mixture by mixing said milled grain with water and protease and optionally at least one enzyme selected from the group consisting of alpha amylase , glucoamylase , cell wall degrading enzyme , and mixtures thereof , wherein the pH of said mixture is optionally adjusted to a pH of about 3.5 to about 6.5 , and incubating said mixture for about 1 to about 3 hours to produce an incubated mixture;said process further comprising:(a) separating said incubated mixture into germ and a liquid slurry, reducing the particle size of materials in said liquid slurry by grinding, adding yeast and amylase to said liquid slurry and fermenting for about 30 to about 72 hours, removing ethanol from said liquid slurry and separating said liquid slurry into fiber and a defibered liquid slurry, and separating said defibered liquid slurry to produce protein solids; or(b) separating said incubated mixture into germ and a liquid slurry, reducing the particle size of materials in said liquid slurry by grinding, adding amylase to said liquid slurry to hydrolyze the starches in said liquid slurry, and separating said liquid slurry into fiber and a defibered liquid slurry, and separating said defibered liquid slurry into protein solids and a solution containing sugars; or(c) separating said ...

PROCESS AND METHOD FOR STILLAGE FERMENTATION

The present invention generally relates to a novel process in which thin stillage is processed to produce algae oil and protein rich biomass as well as other energy rich byproducts. In accordance with a preferred embodiment, thin stillage is removed from an evaporator during the evaporation process to produce mid-stillage. This mid-stillage is preferably routed to a new process where it is directed to a pre-treatment centrifuge to remove suspended solids, sludge and corn oil. Thereafter, the mid-stillage is preferably cooled and then directed to a fermentation tank where the mid-stillage is subject to a batch fermentation process with algae “seed” fed from an algae inoculation system. Once the batch is harvested, the oil-rich algae/mid-stillage is then preferably heated to rupture the cells and liberate the oil. Thereafter, the oil-rich algae/mid-stillage is preferably processed by a centrifuge which produces solids, a light phase oil and a “clean” mid-stillage stream that can be evaporated to a very high level of solids. 1. A process of producing bioproducts following the distillation of biomass , wherein the process comprises the steps of:separating ethanol and whole stillage;routing the whole stillage to a centrifuge;centrifuging the whole stillage to remove solids and to produce a thin stillage, wherein the thin stillage is comprised of 5-8% solids;routing the thin stillage to an evaporator;evaporating water content from the thin stillage to produce mid-stillage, wherein the mid-stillage is comprised of 9-27% solids;separating the mid-stillage into light phase oil, a sludge layer and clarified mid-stillage, wherein the clarified mid-stillage is in water phase with less than 1% suspended solids;cooling the clarified mid-stillage;routing the clarified mid- stillage to an algae fermentation tank;adding algae seed from an algae inoculation system to achieve a starting concentration of 1.5-3.0 grams/L algae;injecting air into the algae fermentation tank to support an ...

Simultaneous Food And Fuel Corn Refining

Food grade products are given priority with a sustainable integrated corn based bio grind refining process. Premium fractions are fractionated for human foods and premium fermentable products. The balance food feedstocks are refined for animal feed with no loss of nutritional value. By-products of refining are used to produce ethanol and other energy products. There is no process waste. The integrated processes can be adapted to new continuous refineries or to optimize or retrofit one or more individual process steps.

MALT SPROUTS EXTRACTS AND THEIR USES

Disclosed are compositions containing malt sprouts fraction extracts, their process of preparation and their uses. 129-. (canceled)30. Composition containing malt sprouts fraction extract , wherein the composition comprises at least 0.5% , and less than 98% of dry matter by total weight of the composition , and comprising:total Nitrogen content from 40 to 140 g/kg of dry matterfree Amino Nitrogen content from 6 to 60 g/kg of dry matter, andnucleic Acid Derivatives such as purines and pyrimidines bases in an amount from 3 to 25 g/kg of dry matter,PUPY content from 1 to 20 g/kg of dry matter,ratio PUPY/Nucleic Acid Derivatives higher than 20%.31. Composition according to claim 30 , consisting of malt sprouts fraction extract wherein the malt sprouts fraction extract comprises at least 0.5% claim 30 , and less than 98% of dry matter by total weight of the malt sprouts fraction extract and comprising:total Nitrogen content from 40 to 80 g/kg of dry matterfree Amino Nitrogen content from 6 to 30 g/kg of dry matter, andnucleic Acid Derivatives such as purines and pyrimidines bases in an amount from 4 to 25 g/kg of dry matter,PUPY content from 3 to 20 g/kg of dry matter,ratio PUPY/Nucleic Acid Derivatives higher than 80%.32. Composition according to claim 30 , consisting of malt sprouts fraction extract wherein the extract is in a liquid form comprising at least 0.5% claim 30 , and less than 1.1% claim 30 , or at least 1.1% claim 30 , and less than 3.5% claim 30 , or at least 3.5% claim 30 , and less than 5% claim 30 , or at least 5% claim 30 , and less than 7% of dry matter by total weight of the malt sprouts fraction extract and comprising:total Nitrogen content from 40 to 80 g/kg of dry matterfree Amino Nitrogen content from 6 to 30 g/kg of dry matter, andnucleic Acid Derivatives such as purines and pyrimidines bases in an amount from 5 to 25 g/kg of dry matter,PUPY content from 4 to 20 g/kg of dry matter,ratio PUPY/Nucleic Acid Derivatives higher than 80%.33. ...

PLANTS WITH MODIFIED TRAITS

The present invention relates, inter alia, to vegetative plant parts, such as from a sp. and/or a plant, which comprise a total fatty acid (TFA) content which comprises fatty acids esterified in the form of triacylglycerols (TAG) and fatty acids in the form of lipids other than TAG, wherein the vegetative plant parts comprise greatly increased levels of TFA, for example a TFA content of about 5% (w/w dry weight). The present invention also relates to the use of the vegetative plant parts as a feedstuff, and/or to produce a feedstuff, for animal consumption. 1. A process for producing a feedstuff for an animal , the process comprising the steps of{'i': Sorghum', 'Zea mays, '(i) harvesting vegetative plant parts from a sp. and/or a plant, the vegetative plant parts comprising a total fatty acid (TFA) content which comprises fatty acids esterified in the form of triacylglycerols (TAG) and fatty acids in the form of lipids other than TAG, wherein the vegetative plant parts comprise a TFA content of about 5% (w/w dry weight) and preferably has a TAG/TFA Quotient (TTQ) of between 0.01 and 0.60, or between 0.60 and 0.84, or between 0.84 and 0.95, and one or more of the steps'}(ii) admixing the harvested plant parts with at least one other feed ingredient,(iii) baling the harvested plant parts,(iv) processing the harvested plant parts, preferably by chopping, cutting, drying, pressing or pelleting the plant parts, into a form that is suitable for consumption by the animal, and(v) storing the harvested plant parts under conditions of reduced oxygen for a period of time such that at least some of the carbohydrates in the plant parts are fermented to organic acids.3. The process of or , in which one or more or all of the following features apply:{'i': Sorghum', 'Zea mays, '(i) the vegetative plant parts are leaves and/or stems or parts thereof which comprise one or more of an increased carbon content, an increased energy content, an increased soluble protein content, a reduced ...

EUGLENA DERIVED COMPOSITION HAVING BIOMASS AND IMMUNE RESPONSE INDUCING COMPONENTS

A composition includes a whole cell biomass and cellular components, including beta-1,3-glucan as at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers. Residual media remains from a heterotrophic fermentation process that produced the biomass. The composition may be in different delivery forms and include added immune response inducing components. 1. A composition , comprising:{'i': Euglena', 'Euglena, 'a whole cell biomass, including beta-1,3-glucan comprising at least about 90 percent linear, unbranched beta-1,3-glucan polysaccharide polymers having an average molecular weight of about 1.2 to 580 kilodaltons (kDa) and beta-glucan polymer chains having a polymer length of about 7.0 to 3,400 glucose monomers, wherein the whole cell biomass comprises at least 30 percent beta-1,3-glucan;'}{'i': 'Euglena', 'residual media remaining from a heterotrophic fermentation process that produced the whole cell biomass and including an added immune response inducing component, and wherein the composition is in the form of a capsule, a tablet, a powder, a lotion, a gel, a stick pack, a liquid solution, a liquid suspension, a gummy, a multivitamin, a health shake, a health bar or a cookie.'}2Echinaceaginsengbilobaganoderma, astragaluspapaya. The composition according to claim 1 , wherein said added immune response inducing component comprises one or more of vitamin C claim 1 , claim 1 , aloe claim 1 , golden seal claim 1 , claim 1 , garlic claim 1 , bell peppers claim 1 , ginger claim 1 , tumeric claim 1 , gingko claim 1 , cat's claw claim 1 , claim 1 , humic or fulvic acids claim 1 , resveratrol or other polyphenols claim 1 , broccoli claim 1 , spinach claim 1 , yogurt claim 1 , almonds claim 1 , honey claim 1 , green tea claim 1 , claim 1 , kiwi claim 1 , poultry claim 1 , shrimp claim 1 , ...

GRANULATED FEED SUPPLEMENT AND METHODS FOR MAKING AND USING

Disclosed herein are embodiments of a feed supplement comprising silica, mineral clay, glucan and mannans, wherein each particle of the feed supplement comprises silica, mineral clay, glucan and mannans. The feed supplement may be a granular feed supplement, and the granules may have at least one dimension between 4 mesh and 100 mesh size. In some embodiments, at least 40% of the granular feed supplement has at least one dimension greater the 100 mesh. Administration of the feed supplement to an animal may increase expression of one or more immune system biomarkers in that animal relative to animals not administered the composition. 1. A granular composition , comprising silica , mineral clay , glucan and mannans.2. The granular composition of claim 1 , each granule comprising a substantially homogenous blend of silica claim 1 , mineral clay claim 1 , glucan and mannans.3. The granular composition of claim 1 , the granular composition comprising greater than 40% by weight granules having at least one dimension between 0.15 mm and 4.8 mm.4. The granular composition of claim 3 , wherein the granular composition comprises greater than 90% by weight granules having at least one dimension between 0.15 mm and 2 mm.5. The granular composition of claim 1 , the granular composition comprising from greater than 0% to 100% granules by weight and from 0% to no more than 60% particles by weight claim 1 , the granules having at least one dimension between 0.15 mm and 2 mm claim 1 , and the particles having at least one dimension of less than 0.15 mm.6. The granular composition of claim 1 , comprising plural granules claim 1 , each granule comprising silica claim 1 , mineral clay claim 1 , glucan and mannans claim 1 , the granular composition having a mineral coefficient of variation of from 0% to 10% claim 1 , a proximate coefficient of variation of from 0% to 20% claim 1 , or both.7. The granular composition of claim 1 , wherein at least 90% of the granules have at least one ...

EXTREME CROP PROCESSING AFTER ENSILING

A method of preparing includes storing cut crop material in an accumulation having an oxygen barrier. The cut crop material ferments within the accumulation to form a silage material. After the fermentation of the silage material, the silage material is mechanically macerated with a macerator, and then fed to ruminant animals. 1. A method of preparing silage , the method comprising:storing cut crop material in an accumulation having an oxygen barrier;fermenting the cut crop material within the accumulation to form a silage material; andmacerating the silage material after fermentation with a mechanical macerator.2. The method set forth in claim 1 , wherein macerating the silage material includes high intensity crop processing of the silage material resulting in cell wall rupture and the release of intracellular solubles of the silage material.3. The method set forth in claim 1 , further comprising cutting standing crop material in a field to provide the cut crop material.4. The method set forth in claim 1 , further comprising drying the cut crop material in the field to achieve a desired initial moisture content.5. The method set forth in claim 1 , further comprising raking the cut crop material into a windrow.6. The method set forth in claim 1 , further comprising collecting the cut crop material in the field.7. The method set forth in claim 6 , further comprising chopping the cut crop material while collecting the cut crop material in the field.8. The method set forth in claim 1 , further comprising feeding the silage material to an animal after macerating the silage material.9. The method set forth in claim 1 , wherein the accumulation having the oxygen barrier includes one of a bale wrapped with the oxygen barrier claim 1 , a bunker silo covered with the oxygen barrier claim 1 , or an oxygen limiting tower silo.10. The method set forth in claim 1 , wherein the oxygen barrier is a plastic covering operable to block transfer of oxygen therethrough.11. A method of ...

Date Pit Composition for the Treatment of Animals

A method of treating or preventing bacterial infection in an animal comprises administering an effective amount of degraded date pits. Degraded date pits can be produced by the treatment of date pits with fungi. 112-. (canceled)13. A method for producing an antibacterial feed additive for treating a bacterial infection in an animal subject in need thereof , the method comprising subjecting date pits to solid state degradation using a fungus to form a fungi-degraded date pit composition.14SalmonellaCampylobacterShigellaEscherichia. The method as claimed in claim 13 , wherein the bacterial infection is caused by at least one of species claim 13 , species claim 13 , species claim 13 , and species.15Trichoderma reeseiTrichoderma reesei. The method as claimed in claim 13 , wherein the fungus is and wherein the fungi-degraded date pit composition is in the form of -degraded date pit composition.16. The method as claimed in claim 13 , wherein the fungi-degraded date pit composition comprises mannan-oligosaccharides (MOS).17. The method as claimed in claim 13 , wherein the fungi-degraded date pit composition comprises free mannose.18. The method as claimed in claim 13 , wherein the antibacterial feed additive is in the form of a powder.19. A method for preparing an antibacterial feed composition for use in treating a bacterial infection in an animal subject in need thereof claim 13 , the method comprising subjecting date pits to solid state degradation using a fungus to form a fungi-degraded date pit composition claim 13 , and mixing the fungi-degraded date pit composition with an animal feed composition.20SalmonellaCampylobacterShigellaEscherichia. The method as claimed in claim 19 , wherein the bacterial infection is caused by at least one of genus claim 19 , genus claim 19 , genus claim 19 , and genus.21Trichoderma reeseiTrichoderma reesei. The method as claimed in claim 19 , wherein the fungus is and wherein the fungi-degraded date pit composition is in the form of - ...

METHOD FOR THE SIMULTANEOUS PRODUCTION OF ETHANOL AND A FERMENTED, SOLID PRODUCT

The invention relates to a method for the simultaneous production of a fermented, solid product and ethanol comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and/or polysaccharides and live yeast in a dry matter ratio of from 2:1 to 100:1, and water; 2) fermenting the mixture resulting from step (1) under conditions where the water content in the initial mixture does not exceed 65% by weight, for 1-36 hours at a temperature of about 25-60° C. under anaerobic conditions; 3) incubating the fermented mixture resulting from step (2) for 0.5-240 minutes at a temperature of about 70-150° C.; and 4) separating wet fermented, solid product from the fermented mixture resulting from step (3); further comprising either a) that the fermentation in step (2) is performed in one or more interconnected paddle worm or continuous worm conveyers with inlet means for the fermentation mixture and additives and outlet means for the ferment as well as control means for rotation speed, temperature and pH, or b) that one or more processing aids are added in any of steps (1), (2) and (3) and further comprising a step of 5) separating crude ethanol from the fermented mixture in step (2) by vacuum and/or in step (3) by vacuum or by injection of steam and condensing the surplus stripping steam. The invention further relates to the products of this method as well as uses thereof. 125.-. (canceled)26. A method for the simultaneous production of a fermented , solid product and ethanol comprising:providing an initial mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and/or polysaccharides, and live yeast in a dry matter ratio of from 2:1 to 100:1, and water, wherein the water content in the initial mixture does not exceed 65% by weight of the initial mixture;fermenting the initial mixture for 1-36 hours at a temperature of about 25-60 ° C. under anaerobic conditions to ...

HIGH QUALITY PROTEIN CONCENTRATE FROM CANOLA MEAL

The present invention describes fungal conversion methods for reduction of glucosinolates (GLS), fiber and residual sugars to increase the protein content and nutritional value of canola meal, including canola-based protein products (CBPP) generated by said methods for use in animal feeds. 1. A method of producing a protein concentrate from canola using fermentation comprising:{'i': Trichoderma reesei', 'Fusarium venenatum;, 'contacting canola containing substrate with a microbe selected from the group consisting of and'}incubating the contacted substrate for sufficient time, and at a pH of between about 4.0 to about 6.0, to convert the carbohydrates contained in said substrate into protein to form a protein concentrate; andisolating the resulting protein concentrate,wherein the resulting protein concentrate exhibits a reduction in glucosinolate concentration of between about 69% to about 98%.2. The method of claim 1 , wherein fermentation is solid state fermentation (SSF) or submerged fermentation (SMF) or a hybrid of SSF and SMF.3Trichoderma reesei.. The method of claim 1 , wherein the microbe is4. The method of claim 2 , wherein the fermentation is SSF.5. The method of claim 4 , wherein the moisture content of the substrate is between about 40% to about 60%.6. The method of claim 1 , where the protein concentration of the substrate increase between about 15% to about 23%.7. The method of claim 1 , wherein the substrate is thermally treated prior to contact with the microbe.8. The method of claim 1 , wherein the substrate is hexane extracted (HE) or cold pressed (CP).9. The method of claim 1 , wherein incubation is carried out without perturbing the substrate.10. A protein concentrate produced by the method of .11. A method of producing a non-animal based protein concentrate comprising:pelletizing a substantially dry substrate selected from the group consisting of cereal grains, bran, sawdust, peat, oil-seed materials, wood chips, and combinations thereof;passing ...

Microbial Production of Protein and PHB by Alcohol Utilizing Bacteria

Microorganisms and methods are provided for producing biomass that includes PHB and protein in weight ratios and polymer lengths that are beneficial in feed and nutritional supplement compositions. The compositions also may be used for improvement in feed compositions that improve survivability of livestock and aquaculture species. 1. A non-naturally occurring microorganism that produces about 1% to about 99.9% less of a polyhydroxyalkanoate (PHA) product by weight and about 1% to about 250% more protein by weight than the parent microorganism from which the non-naturally occurring microorganism is derived.2. A non-naturally occurring microorganism according to claim 1 , wherein the weight ratio of PHA to protein produced by the microorganism comprises about 1:1000 to about 2:1.3. A non-naturally occurring microorganism according to claim 2 , wherein the weight ratio of PHA to protein produced by the microorganism comprises about 1:1000 to about 1:6.4. A non-naturally occurring microorganism according to claim 1 , wherein the PHA product comprises poly-β-hydroxybutyrate (PHB).5Methylomonas, Methylobacter, Methylococcus, Methylosinus, Methylocyctis, Methylomicrobium, Methanomonas, Methylophilus, Methylobacillus, Methylobacterium, Hyphomicrobium, Xanthobacter, Bacillus, Paracoccus, Nocardia, Arthrobacter, Rhodopseudomonas, Pseudomonas, Candida, Hansenula, Pichia, Torulopsis, Vibrio, Escherichia, Alcaligenes, Ralstonia, Rhodobacter, Saccharomyces, Cupriavidus, Sinorhizobium, Mucor, Bradyrhizobium, Yarrowia, Azotobacter, Synechocystis, Rhodotorula, Aeromonas, Magnetospirillum, Haloferax, CaryophanonAllochromatium.. A non-naturally occurring microorganism according to claim 1 , wherein the microorganism is of the genus claim 1 , or6Methylobacterium.. A non-naturally occurring microorganism according to claim 5 , wherein the microorganism is a7Methylobacterium extorquens.. A non-naturally occurring microorganism according to claim 6 , wherein the microorganism is8. A non- ...

METHOD FOR DRYING BIOMASS

It has been found in accordance with the invention that a biomass comprising an oxidation-sensitive material of value may be particularly readily converted into a particulate, flowable composition if a silica, especially a hydrophilic or hydrophobic silica, is added as additive. 115-. (canceled)16. A method for preparing a particulate free-flowing biomass comprising an oxidation-sensitive material of value , wherein said method comprises:a) obtaining a fermentation broth comprising biomass;b) optionally concentrating said fermentation broth to produce a concentrated broth;c) drying either said fermentation broth or said concentrated broth;d) adjusting the amount of silica in said fermentation broth or said concentrated broth either before or after the drying of step c) so that the concentration of silica in said particulate free-flowing biomass is 0.1-8% by weight.17. The method of claim 16 , wherein the silica is a hydrophilic silica.18. The method of claim 17 , wherein the hydrophilic silica has a specific surface area of 150 to 600 m/g and a dioctyl adipate absorption value of 2-3.5 ml/g.19. The method of claim 16 , wherein the silica is a hydrophobic silica.20. The method of claim 19 , wherein the hydrophobic silica has a methanol wettability of at least 40%.21. The method of claim 16 , wherein the oxidation-sensitive material of value is an unsaturated fatty acid.22. The method of claim 16 , wherein the silica is adjusted so that the concentration of silica in the particulate biomass is 0.2 to 6% by weight.23. The method of claim 16 , wherein the biomass comprises cells from the taxon Labyrinthulomycetes.24. The method of claim 16 , wherein the fermentation broth has a solids content of 10 to 50% by weight claim 16 , and is converted to the particulate biomass by drying.25. The method of claim 16 , wherein biomass is dried by a thermal process and silica is added during the drying process.26. The method of claim 16 , wherein:a) the oxidation-sensitive material ...

Use of Enzymes to Condition Ruminant Feedstocks

A method of improving milk yield in a ruminant animal a ruminant ration with an aqueous is presoaked with a solution of a composition of enzymes comprising a xylanase and a ferulic acid esterase. 1. A method of improving the digestibility of an equine or ruminant animal ration , comprising the step of treating the equine or ruminant ration by wetting with an aqueous solution of a composition of enzymes comprising a xylanase and a ferulic acid esterase , resulting in improvement of animal performance.2. The method of claim 1 , wherein the treated ration is allowed to remain in contact with the composition of enzymes for a residence period of time prior to being fed to the animal.3. The method of claim 1 , wherein the xylanase is present in the composition of enzymes in an amount between 5% and 95% and the ferulic acid esterase is present in the composition of enzymes in an amount between 1% and 20%.4. The method of claim 1 , wherein the ration comprises at least one component selected from the group consisting of soya forage claim 1 , sugarcane bagasse claim 1 , wheat straw claim 1 , rice straw claim 1 , green fodder claim 1 , and a total mixed ration.5. The method of claim 1 , wherein the ration is soaked in the aqueous solution of a composition of enzymes and the animal is fed both the ration and any remaining aqueous solution.6. A method of reducing the NDF in an equine or ruminant animal ration claim 1 , comprising the step of treating the ration by wetting with an aqueous solution of a composition of enzymes comprising a xylanase and a ferulic acid esterase.7. A method of increasing milk yield of a ruminant animal claim 1 , comprising the step of treating a ruminant ration by wetting with an aqueous solution of a composition of enzymes comprising a xylanase and a ferulic acid esterase.8. The method of claim 7 , wherein the ruminant animal is a dairy animal in either the early lactation phase claim 7 , the mid-lactation phase or the late lactation phase.9. The ...

METHOD OF SEPARATING LIPIDS FROM A LYSED LIPIDS CONTAINING BIOMASS

The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass. 116-. (canceled)17. A method of separating a polyunsaturated fatty acids (PUFAs)-containing lipid from the debris of a biomass , comprising the following steps:a) providing a suspension of a biomass comprising cells which contain a PUFAs-containing lipid;b) lysing the cells of the biomass;c) heating the suspension obtained in step b) to a temperature of 80° C. to 100° C., and adjusting the pH to a value of 9.0 to 11.5;d) maintaining the temperature and pH value in the ranges of paragraph c) for at least 10 hours.18. The method of claim 17 , wherein claim 17 , in step c) the suspension obtained in step b) is heated to a temperature of 85° C. to 95° C. claim 17 , and the pH is adjusted to a value of 9.0 to 11.0 and in step d) claim 17 , the temperature and pH are maintained for a period of 20 to 36 hours.19. The method of claim 17 , wherein lysing of the cells of the biomass is carried out enzymatically claim 17 , mechanically claim 17 , chemically and/or physically.20. The method of claim 19 , wherein lysing of the cells of the biomass comprises:a) heating the suspension of the biomass to a temperature of between 50° C. and 70° C., adding a cell wall-degrading enzyme, and adjusting the pH to a value at which the enzyme is active;b) maintaining the temperature and pH in the ranges of paragraph a) for at least one hour.21. The method of claim 17 , wherein less than 1 wt.-% claim 17 , of organic solvents and/or less than 1 wt.-% claim 17 , of chloride are used in the method.22. The method of claim 17 , wherein after lysing of the cells claim 17 , the suspension is concentrated to a total dry matter content of 30 to 60 wt.-%.23. The method of claim 17 , comprising as a further step the harvesting of the PUFAs containing oil.24. The method of claim 23 , wherein harvesting of the PUFAs containing oil comprises neutralizing the demulsified ...

SUBTERRANEAN MICROALGAE FOR PRODUCTION OF MICROBIAL BIOMASS, SUBSTANCES, AND COMPOSITIONS

The invention pertains to a method for synthesizing a product of interest by culturing a microalgal cell obtained from a subterranean habitat for producing the product of interest. The microalgal cell obtained from a subterranean habitat can be cultured in the dark, in light, in low nutrition, or nutrient rich conditions for at least a portion of production cycle. A combination of these conditions can be used to specifically manipulate a microalgal cell culture to produce a product of interest. The product of interest can be a water-soluble carotenoid, for example, a water-soluble carotenoid produced by culturing an algae belonging to the genus or a capsular exopolysaccharide produced by culturing an algae belonging to the genus . Compositions containing the water-soluble carotenoid, for example, as sunscreen and compositions containing the exopolysaccharide, for example, as moisturizing cream are also described. 1. A method for synthesizing a product of interest , the method comprising:providing a culture medium comprising one or more biogenic substances and one or more minerals,providing a subterranean microalgal cell that produces the product of interest;culturing the microalgal cell in the culture medium to produce a microalgal culture from the microalgal cell;optionally, culturing the microalgal culture;isolating the microalgal cells from the microalgal culture; andpurifying the product of interest from the microalgal cells.2. The method of claim 1 , wherein the culture medium comprises one or more minerals providing ions selected from Ca claim 1 , K claim 1 , Mg claim 1 , PO4 claim 1 , SO claim 1 , NO claim 1 , NH claim 1 , HCOand Cl Fe claim 1 , Fe claim 1 , Fe-oxides claim 1 , Si-oxides (including amorphous silica); and the one or more biogenic substances comprise organic or inorganic carbon and nitrogen.3. The method of claim 2 , wherein the organic carbon is present in a monomeric sugar claim 2 , in a sugar alcohol claim 2 , or in an organic acid selected ...

USE OF INOCULANTS AND ENZYMES TO INCREASE NUTRIENT RELEASE IN ANIMAL DIETS

Disclosed are methods for improving the digestibility of high-moisture grain feed and/or rehydrated grain feed for animals which comprises a) processing the grain feed into fragments and b) contacting the grain feed fragments of step (a) with at least one starch hydrolase which is stable and active at a pH less than 5.0, optionally at least one protease, in combination with at least one inoculant comprising at least one bacterial strain. 1. A method for improving the digestibility of high-moisture grain feed and/or rehydrated grain feed for animals which comprises a) processing the grain feed into grain feed fragments and b) contacting the grain feed fragments of step (a) with at least one starch hydrolase that is both stable and active at a pH less than 5.0 in combination with at least one inoculant comprising at least one bacterial strain.2. The method of wherein the starch hydrolase has a starch binding domain wherein said starch hydrolase is capable of hydrolyzing raw starch.3. The method of where in the starch hydrolase is selected from the glycoside hydrolase family 13 and/or 15.4. The method of claim 1 , or wherein the starch hydrolase is selected from the group consisting of at least one alpha amylase or at least one glucoamylase.5. The method of claim 1 , claim 1 , or wherein step (b) further comprises a protease.6. The method of wherein step (b) further comprises a protease.7. The method of or wherein the protease is an endopeptidase.8. The method of wherein the protease is an endopeptidase.9. The method of or wherein the endopeptidase is selected from the group consisting of metallopeptidases claim 5 , serine proteases claim 5 , threonine proteases and aspartic proteases.10. The method of wherein the endopeptidase is selected from the group consisting of metallopeptidases claim 7 , serine proteases claim 7 , threonine proteases and aspartic proteases.11lactobacillus. The method of claim of claim 7 , claim 7 , or wherein the at least one inoculant ...

METHOD FOR EXTRACTING A MICROBIAL OIL COMPRISING POLYUNSATURATED FATTY ACIDS FROM A FERMENTATION BROTH CONTAINING OLEAGINOUS MICROORGANISMS

The processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells comprise removing water from the cell fermentation broth or lysed cell composition before demulsification is conducted. Such a process has the benefits of reduced demulsification time and reduced salt use. Microbial oil comprising one or more PUFAs can be recovered from microbial cells by the process. 1. A method for enhancing demulsification of a fermentation broth containing lysed oleaginous microorganisms , comprising:a) removing water from the fermentation broth wherein the volume of the fermentation broth containing lysed oleaginous microorganisms is less than 60% of its original volume; andb) demulsify the fermentation broth by heating to a temperature of 60° C. to 110° C.2. The method of claim 1 , wherein the demulsification is enhanced by reducing the time of demulsification to at least ⅓ of the time that is needed for demulsification when step a) is not performed.3. The method of claim 1 , further comprising c) recovering an oil from the fermentation broth.4. The method of claim 3 , wherein the recovering of oil is performed without the use of a solvent.5. The method of claim 4 , wherein the amount of oil recovered is increased by at least 7% compared to the same method when step a) is not performed.6. The method of claim 1 , wherein the volume of the fermentation broth containing lysed oleaginous microorganisms in step a) is reduced to less than 70% claim 1 , and preferably less than 80% of its original volume.7. The method of claim 1 , wherein removal of water in step a) is performed by heating the fermentation broth at a temperature not more than 110° C. claim 1 , preferably between 70° C. to 100° C. claim 1 , and more preferably between 80° C. and 90° C.8. The method of claim 1 , wherein step b) comprises adding an alkalizing agent claim 1 , preferably caustic soda.9. The method of claim 8 , wherein pH of the ...

Process and Method for Optimizing Production of Food and Feed

A process includes microbially degrading harvested polyculture plant material to form a concentrated microbial biomass and providing the concentrated microbial biomass to an intermediary animal for consumption. The process may also be directed to producing a product animal which includes providing a growth area having an outlet for waste and providing a harvested plant material collection area having an outlet for degradation products. The process may also include providing a microbial growth system for producing a bacterial biomass and directing at least some waste from the outlet of the product animal growth area to the harvested plant material collection area. The process may also include directing at least some degradation products to the microbial growth system, directing some of the microbial biomass produced in the microbial growth system to an intermediary animal for consumption, and directing the intermediary animal to a product animal growth area.

METHOD FOR CONVERTING FOOD WASTE AND OTHER BIOLOGICAL WASTE INTO INVERTEBRATE FEED

Biological waste such as food, organic or other biologically-derived waste is converted into shelf-stable and health-safe invertebrate feed. The method for converting includes pre-treating waste by fragmenting, reducing microbial contaminants, optionally amending with components that optimize fermentation, inoculating with microorganisms and mixing. Fermentation takes place in a bioreactor and produces fermentation leachate and solid fermentate. In the post-treatment steps, the solid fermentate is separated from the fermentation leachate. The solid fermentate is ground, dewatered and milled. The solid fermentate can be used as an invertebrate feed with or without further processing.

METHOD FOR PRODUCING FEED OR PET FOOD INGREDIENT USING CRUSHED PINEAPPLE LEAVES AND STALKS AND FEED OR PET FOOD INGREDIENT USING CRUSHED PINEAPPLE LEAVES AND STALKS

Granulated pineapple leaves and stalks dried to correspond to a predetermined fermentation condition are fermented in an anaerobic bacteria fermentative environment in a predetermined room temperature with % or more and less than % moisture content, or to % moisture content maintained therein, to form a pineapple-based feed or pet food ingredient of dryness and low-fermentation condition. 1. A method for producing a pineapple-based feed or pet food ingredient comprising:separating from a pineapple fruit grown in a pineapple field to a pineapple leaf and a pineapple stalk,crashing the pineapple leaves and stalks during or after harvest,drying the crushed pineapple leaves and stalks in a dryer to form dried crushed pineapple leaves and stalks contained moisture,storing the dried crushed pineapple leaves and stalks with the moisture maintained therein in a moisture-preservative vessel in an anaerobic bacteria fermentative environment, andcontinuously fermenting the dried crushed pineapple leaves and stalks with the moisture maintained therein,Wherein the drying process performs a condition which is dried until less than 50% of moisture content,an ingredient which is the dried crushed pineapple leaves, the dried crushed pineapple stalks, or a mixture thereof is fermented in a predetermined temperature and in the anaerobic bacteria fermentative environment while the moisture is maintained therein, anda pineapple-based feed or pet food ingredient is formed by way of the dryness and low-fermentation condition which is thrown off smell favored by livestock and pets, the pineapple-based feed or the pet food ingredient being stayed behind smell favored by livestock and pets.2. The method of claim 1 , wherein the ingredient is fermented with moisture content of 15% or more and less than 50% to form the low-fermentation condition which is thrown off smell favored by livestock and pets.3. The method of claim 1 , wherein the ingredient is fermented with 20 to 40% moisture content ...

CARBOHYDRATE-ENRICHED RECOMBINANT MICROORGANISMS

The present disclosure relates to recombinant microorganisms engineered for enhanced production of a desired carbohydrate, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present disclosure also provides related methods. 1. A recombinant methanotrophic bacterium , comprising an exogenous nucleic acid that encodes a gluconeogenesis enzyme ,wherein the encoded gluconeogenesis enzyme is one or more of a pyruvate carboxylase, a phosphoenolpyruvate carboxykinase, an enolase, a phosphoglycerate mutase, a phosphoglycerate kinase, a glyceraldehyde-3-phosphate dehydrogenase, a Type A aldolase, a fructose 1,6-bisphosphatase, a phosphofructokinase, a phosphoglucose isomerase, a hexokinase, and a glucose-6-phosphate,{'i': Methylomonas, Methylobacter, Methylococcus, Methylosinus, Methylocystis, Methylomicrobium, Methanomonas', 'Methylocella, 'wherein the methanotrophic bacterium is selected from the group consisting of , and , and'}{'sub': '1', 'wherein the recombinant methanotrophic bacterium grown on a Csubstrate feedstock is capable of producing glucose at a level that is greater than that produced by the parent methanotrophic bacterium.'}2. The recombinant methanotrophic bacterium of claim 1 , wherein the Csubstrate feedstock comprises natural gas or methane.3. The recombinant methanotrophic bacterium of claim 1 , wherein the methanotrophic bacterium is an obligate methanotrophic bacterium.4. The recombinant methanotrophic bacterium of claim 1 , wherein the methanotrophic bacterium is a facultative methanotrophic bacterium.5Methylococcus capsulatusMethylomonasMethylosinus trichosporiumMethylosinus sporium, Methylocystis parvus, Methylomonas methanica, Methylomonas albus, Methylobacter capsulatusMethylomonas flagellataMethylacidiphilum infernorum, Methylacidiphdum fumariolicum, Methylomicrobium alcaliphdumMethyloacida kamchatkensis.. The recombinant methanotrophic bacterium of claim 1 , wherein the methanotrophic ...

FEED AND PRODUCTION METHOD THEREOF

A feed which has a stronger effect of increasing the body weight of an organism to be raised than a conventional product, a production method thereof and a raising method using the feed are provided. A feed containing fermented blackcurrant fruit is used. As the blackcurrant fruit, for example, pomace which remains after pressing for juice can be used, and fermented blackcurrant obtained using an species can be used preferably. 17-. (canceled)8. A feed comprising fermented blackcurrant fruit.9. The feed according to claim 8 , comprising 0.01 to 2 mass % of the fermented blackcurrant fruit in terms of the dry mass.10. The feed according to claim 8 , wherein the blackcurrant fruit is pomace of blackcurrant fruit and contains at least a pericarp.11. The feed according to claim 9 , wherein the blackcurrant fruit is pomace of blackcurrant fruit and contains at least a pericarp.12Aspergillus, Bifidobacterium, Penicillium, Chrysosporium, Trichoderma, Acremonium, Candida, Mucor, Rhizopus, MonascusAbsidia.. The feed according to claim 8 , wherein the fermented blackcurrant fruit has been fermented by at least one species selected from the group consisting of species of and13Aspergillus, Bifidobacterium, Penicillium, Chrysosporium, Trichoderma, Acremonium, Candida, Mucor, Rhizopus, MonascusAbsidia.. The feed according to claim 9 , wherein the fermented blackcurrant fruit has been fermented by at least one species selected from the group consisting of species of and14Aspergillus, Bifidobacterium, Penicillium, Chrysosporium, Trichoderma, Acremonium, Candida, Mucor, Rhizopus, MonascusAbsidia.. The feed according to claim 10 , wherein the fermented blackcurrant fruit has been fermented by at least one species selected from the group consisting of species of and15. A method for raising an organism comprising causing an organism to be raised to take the feed according to at least once a week.16. A method for raising an organism comprising causing an organism to be raised to take ...

PROCESSING BIOMASS

Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. 1. A method comprising:reducing recalcitrance of a biomass material to provide a treated biomass material; andconverting the treated biomass material into a plastic or plastic precursor.2. The method of claim 1 , wherein reducing recalcitrance of the biomass material comprises irradiating the biomass material.3. The method of claim 2 , wherein irradiating the biomass material comprises applying a beam of electrons to the biomass material.4. The method of claim 3 , the biomass material is irradiated at a total dosage of between about 10 MRad and about 100 MRad.5. The method of claim 1 , wherein treating further comprises an additional step selected from sonication claim 1 , oxidation claim 1 , pyrolyzing claim 1 , or steam-explosion.6. The method of claim 1 , wherein converting comprises using the treated biomass material as a substrate by microorganisms claim 1 , wherein the microorganisms are capable of generating plastics or plastic precursors claim 1 , to generate plastics or plastic precursors.7. The method of claim 6 , wherein the method generates one or more of xanthan claim 6 , alginate claim 6 , cellulose claim 6 , cyanophycin claim 6 , poly(gamma-glutamic acid) claim 6 , levan claim 6 , hyaluronic acid claim 6 , and polyhydroxyalkanoates.8. The method of claim 6 , wherein the method generates at least one of Poly-β-hydroxybutyrate and Polyβ-hydroxyvalerate.9. The method of claim 1 , wherein the biomass comprises cellulosic and/or lignocellulosic materials.10. A method comprising:converting a treated biomass material into a plastic or plastic precursor, wherein the treated biomass has been treated to reduce the recalcitrance of the biomass material.11. The method of claim 10 , wherein converting comprises using the treated biomass material as a substrate by microorganisms claim 10 , wherein the ...

Method and Process for Acidic Recycling of Protein Waste

A method for recycling protein waste and producing an edible product for animal consumption comprises grinding animal carcasses or parts thereof and thereafter acidifying the waste. The acidified waste may then be emulsified, followed by a heat shock and then fed to animals. Alternatively, a second emulsification may be employed prior to being used for animal consumption. The emulsified products may be provided to animals as a liquid, or dried to a paste prior to use. 1. A process for making a product for animal consumption , said process comprising:a) grinding a protein source and adding acid to the ground protein source to generate an acidic digest medium exhibiting biological stability at ambient temperatures up to at least 21 days,b) mixing a portion of the acidic digest medium with an additional amount of ground protein to form an acidic mixture,c) emulsifying the acidic mixture to form an acidic digest product said acidic digest product exhibiting biological stability at ambient temperatures up to at least 21 days.2. The process of further comprising optionally subjecting the acidic digest product to a heat shock step to retard bacterial growth prior to storing.3. The process of claim 2 , further comprising a second emulsifying step comprising emulsifying the acidic digest product.4. The process of claim 3 , said second emulsifying step following the heat shock step.5. A process for making a product for animal consumption claim 3 , said process comprising mixing an acid with a ground protein source to form an acidic digest medium claim 3 , said acidic digest medium capable of maintaining its biological stability for up to 90 days.6. The process of further comprising adding an additional protein source to the acidic digest medium and emulsifying the resulting acidic mixture to form an acidic digest product followed by heat shocking the acidic digest product resulting in enhanced biological stability.7. The acidic digest medium of claim 1 , said acidic digest ...

Integration of First and Second Generation Bioethanol Processes

The present invention presents an alternative to the direct implementation of an industrial scale second generation bioethanol process with the integration of the second generation into the existing first generation bioethanol processes, which aims to reduce the current barriers to process change/investments. In particular, the present invention relates to an integrated second generation process for producing bioethanol comprising at least one fungal cultivation stage for producing ethanol and fungal biomass. The present invention also relates to a novel fungal biomass, rich in protein and essential amino acids, which is produced with said integrated second generation process for producing bioethanol for use as a nutritious substitute for human and domestic animal use. 1. An integrated second generation process for producing bioethanol comprising a second generation bioethanol process , which is integrated into a first generation bioethanol process and comprises at least one fungal cultivation stage which produces ethanol and fungal biomass , wherein the second generation process is(a) integrated at the fermentation stage, and/or(b) integrated at the fungal cultivation stage, andwherein the fungi introduced in the fungal cultivation stage are filamentous fungi, selected from the group consisting of food-related strains of Ascomycetes.2RhizopusFusarium venenatium; Aspergillus oryzae; Monascus purpureus, Neurospora intermedia. An integrated second generation process for producing bioethanol according to claim 1 , wherein the filamentous fungi are selected from the group consisting of sp.; and combinations thereof.3Neurospora intermedia.. An integrated second generation process for producing bioethanol according to claim 2 , wherein the filamentous fungi are4. An integrated second generation process for producing bioethanol according to claim 1 , wherein the integrated second generation process yields an at least 2.5% improvement of ethanol production compared to the ...

PROCESS FOR FORMING IRON ENRICHED NUTRITIONAL PRODUCTS

The technology relates to a process for forming a nutritional supplement containing iron by providing a culture medium containing insoluble iron and culturing filamentous fungi in the culture medium to accumulate iron in the filamentous fungi as metabolizable organic iron. The technology also relates to uses of the nutritional supplement as food additive or supplement. 1. A process for forming a nutritional supplement containing iron , the process comprising:providing a culture medium containing insoluble iron; andculturing filamentous fungi in the culture medium to accumulate iron in the filamentous fungi as metabolizable organic iron.2. The process according to wherein the insoluble iron is selected from the group consisting of elemental iron powders claim 1 , atomized iron claim 1 , electrolytic iron claim 1 , H-reduced iron claim 1 , CO-reduced iron claim 1 , carbonyl iron claim 1 , ferric pyrophosphate claim 1 , ferric orthophosphate and iron oxides.3. The process according to wherein the insoluble iron is added to the culture media from about 0.1 gram per liter of media to up to about 10 grams per liter.4. The process according to wherein the insoluble iron is added to the culture media from about 1 gram per liter of media to up to about 3 grams per liter.5Aspergillus oryzaeAspergillus nigerRhizopus orligosporusRhizopus oryzae. The process according to wherein the filamentous fungi is selected from (A.o.) claim 1 , (A.n.) claim 1 , (R.o) or (R.oz).6Aspergillus oryzae. The process according to wherein the filamentous fungi is (A.o.).7. The process according to further including:removing unaccumulated insoluble iron from the culture.8. The process according to further including:harvesting the filamentous fungi to obtain a nutritional supplement containing fungal biomass having at least about 100 mg/kg iron.9. The process according to wherein the nutritional supplement contains at least about 1000 mg/kg iron.10. The process according to wherein the nutritional ...

Method for producing feed additive raw material

Provided is a method for producing a feed additive raw material that can be used as a raw material of a feed additive for improving the palatability of feed and is capable of stably culturing animalcules containing a large amount of amino acids. A method for producing a feed additive raw material, comprising: a first biological treatment step of preparing a first biologically treated liquid containing dispersible bacteria by introducing an organic wastewater containing 40 wt % or more of protein to a first biological treatment tank and performing aerobic biological treatment with bacteria; a second biological treatment step of preparing a second biologically treated liquid by introducing the first biologically treated liquid to a second biological treatment tank and performing activated sludge treatment; and a sludge separation step of taking out part of the tank sludge from the second biological treatment tank as the feed additive raw material.

USE OF AN ENZYMATIC COMPOSITION IN THE FEED OF RUMINANTS

The present invention relates to an enzymatic composition comprising a main substrate, selected from the group consisting of wheat, wheat bran, rapeseed cake and a mixture thereof, fermented with a strain of section , said enzymatic composition having: (i) a xylanase activity greater than or equal to 500 AXC per gram of composition, (ii) a β-glucanase activity of greater than or equal to 500 BGU per gram of composition, and (iii) a cellulase activity greater than or equal to 50 CMC per gram of composition; as well as to its use in the feed of ruminants. 1AspergillusNigri. An enzymatic composition comprising a main substrate , selected from the group consisting of wheat , wheat bran , rapeseed cake , a mixture of wheat and wheat bran , a mixture of wheat and rapeseed cake , a mixture of wheat bran and rapeseed cake , and a mixture of wheat , wheat bran and a rapeseed cake , fermented with a strain of section , said enzymatic composition displaying:(i) a xylanase activity greater than or equal to 500 AXC per gram of composition,(ii) a β-glucanase activity greater than or equal to 500 BGU per gram of composition, and(iii) a cellulase activity greater than or equal to 50 CMC per gram of composition.2AspergillusNigriAspergillus niger.. The enzymatic composition according to claim 1 , wherein the strain of section is a strain of the clade3AspergillusNigriAspergillus tubingensis.. The enzymatic composition according to claim 1 , wherein the strain of section is a strain of the sub-clade4AspergillusNigriAspergillus tubingensisAspergillus neoniger.. The enzymatic composition according to claim 1 , wherein the strain of section is a strain of or5AspergillusNigriAspergillus tubingensis.. The enzymatic composition according to claim 1 , wherein the strain of section is a strain of6AspergillusNigri. The enzymatic composition according to claim 1 , wherein the substrate is fermented with the strain of section by solid-state fermentation.7. The enzymatic composition according to ...

Ergothioneine-containing hen's egg, feed and feeding and raising method for laying hen that lays ergothioneine-containing hen's egg

To provide a production technique for an edible egg that contains a sufficient amount of ergothioneine by using the biological accumulation of the ergothioneine in eggs, and that has antioxidative activity. This ergothioneine-containing egg is obtained from a hen raised by being fed a hen feed for a prescribed time period, the hen feed being manufactured by manufacturing the ergothioneine-containing extract solution from a waste medium used in the production of edible mushrooms and mixing a prescribed amount of the extract solution into a feed for the hen used generally.

SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELL

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. 1221-. (canceled)222Brassica. A plant cell for producing docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) in an esterified form as part of triacylglycerols in the plant cell comprisinga polynucleotide which encodes a Δ4 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ5 desaturase which has the amino acid sequence set forth as SEQ ID NO:18, operably linked to a promoter which directs expression of the polynucleotide in the plant cell;a polynucleotide which encodes a Δ6 desaturase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell; anda polynucleotide which encodes a Δ5/Δ6 bifunctional elongase, operably linked to a promoter which directs expression of the polynucleotide in the plant cell.223BrassicaBrassica napus. The plant cell of which is a plant cell.224Brassica napusBrassica napus. A seed comprising the plant cell of .225Brassica. The plant cell of claim 222 , comprising an exogenous desaturase which desaturates an acyl-CoA substrate.226Brassica napus. The plant seed of claim 224 , comprising an exogenous desaturase which desaturates an acyl-CoA substrate.227Brassica. A plant cell comprising{'i': 'Pavlova lutheri', 'a polynucleotide which encodes a Δ4 desaturase which has the amino acid sequence encoded by a Δ4 desaturase gene from , operably linked to a promoter which directs expression of the ...

CARBOHYDRATE-ENRICHED RECOMBINANT MICROORGANISMS

The present disclosure relates to recombinant microorganisms engineered for enhanced production of a desired carbohydrate, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present disclosure also provides related methods. 1. A recombinant Cmetabolizing microorganism comprising an exogenous nucleic acid selected from the group consisting of an exogenous nucleic acid that encodes a carbohydrate biosynthesis enzyme and an exogenous nucleic acid that encodes an expression control sequence that is operably linked to a nucleic acid encoding a native carbohydrate biosynthesis enzyme , wherein the recombinant Cmetabolizing microorganism is capable of converting a natural gas-derived carbon feedstock into a desired carbohydrate.2. The recombinant Cmetabolizing microorganism of claim 1 , wherein the exogenous nucleic acid encodes a carbohydrate biosynthesis enzyme.3. The recombinant Cmetabolizing microorganism of any of - claim 1 , wherein the natural gas-derived feedstock is natural gas.4. The recombinant Cmetabolizing microorganism of any of - claim 1 , wherein the natural gas-derived feedstock is methane.5. The recombinant Cmetabolizing microorganism of any of - claim 1 , wherein the recombinant Cmetabolizing microorganism is capable of producing the desired carbohydrate at a level that is at least 10% greater than that produced by the native Cmetabolizing microorganism.6. The recombinant Cmetabolizing microorganism of any of - claim 1 , wherein the Cmetabolizing microorganism is a methanotroph.7. The recombinant Cmetabolizing microorganism of any of - claim 1 , wherein the desired carbohydrate is selected from the group consisting of a polysaccharide claim 1 , a disaccharide claim 1 , and a monosaccharide.8. The recombinant Cmetabolizing microorganism of claim 7 , wherein the desired carbohydrate is a polysaccharide that is a β-glucan.9. The recombinant Cmetabolizing microorganism of any of - claim 7 , wherein the ...

Filamentous fungal biomats, methods of their production and methods of their use

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

GAS FERMENTATION FOR THE PRODUCTION OF PROTEIN OR FEED

The invention provides animal feed comprising microbial biomass and methods of producing animal feed by culturing a microorganism to produce microbial biomass. In particular, the invention relates to animal feed produced by fermentation of a gaseous substrate comprising one or more of CO, CO, and H, especially by a Gram-positive, anaerobic, and/or microorganism. 1. Animal feed comprising microbial biomass and at least one excipient , wherein the microbial biomass comprises a microorganism grown on a gaseous substrate comprising one or more of CO , CO , and H.2. The animal feed of claim 1 , wherein the microorganism is Gram-positive.3. The animal feed of claim 1 , wherein the microorganism is acetogenic and/or carboxydotrophic.4. The animal feed of claim 1 , wherein the microorganism is anaerobic.5Clostridium.. The animal feed of claim 1 , wherein the microorganism is a member of the genus6Clostridium autoethanogenum, Clostridium ljungdahlii, Clostridium ragsdaleiClostridium coskatii.. The animal feed of claim 1 , wherein the microorganism is or is derived from claim 1 , or7. The animal feed of claim 1 , wherein the microorganism is not methanotrophic.8. The animal feed of claim 1 , wherein the gaseous substrate comprises CO.9. The animal feed of claim 1 , wherein the gaseous substrate does not comprise methane.10. The animal feed of claim 1 , wherein the gaseous substrate is or is derived from industrial waste gas claim 1 , industrial off gas claim 1 , or syngas.11. The animal feed of claim 1 , wherein the animal feed is suitable for feeding to livestock or pets.12. The animal feed of claim 1 , wherein the animal feed is suitable for feeding to one or more of beef cattle claim 1 , dairy cattle claim 1 , pigs claim 1 , sheep claim 1 , goats claim 1 , horses claim 1 , mules claim 1 , donkeys claim 1 , deer claim 1 , buffalo/bison claim 1 , llamas claim 1 , alpacas claim 1 , reindeer claim 1 , camels claim 1 , bantengs claim 1 , gayals claim 1 , yaks claim 1 , chickens ...

Processes of Treating Grain

Processes of treating grain (e.g., corn), involving milling the grain to produce milled grain wherein the grain germ remains intact in the milled grain, and producing a mixture by mixing the milled grain with water and at least one enzyme selected from the group consisting of protease, alpha amylase, glucoamylase, cell wall degrading enzyme, and mixtures thereof, wherein the pH of the mixture is optionally adjusted to a pH of about 3.5 to about 6.5, and incubating the mixture for about 1 to about 3 hours to produce an incubated mixture. 1. A process of treating grain , said process comprising milling said grain to produce milled grain wherein the grain germ remains intact in said milled grain , and producing a mixture by mixing said milled grain with water and at least one enzyme selected from the group consisting of protease , alpha amylase , glucoamylase , cell wall degrading enzyme , and mixtures thereof , wherein the pH of said mixture is optionally adjusted to a pH of about 3.5 to about 6.5 , and incubating said mixture for about 1 to about 3 hours to produce an incubated mixture.2. The process of claim 1 , further comprising separating said incubated mixture into germ and a liquid slurry claim 1 , reducing the particle size of materials in said liquid slurry by grinding claim 1 , adding yeast and amylase to said liquid slurry and fermenting for about 30 to about 72 hours claim 1 , removing ethanol from said liquid slurry and separating said liquid slurry into fiber and a defibered liquid slurry claim 1 , and separating said defibered liquid slurry to produce protein solids.3. The process of claim 1 , further comprising separating said incubated mixture into germ and a liquid slurry claim 1 , reducing the particle size of materials in said liquid slurry by grinding claim 1 , adding amylase to said liquid slurry to hydrolyze the starches in said liquid slurry claim 1 , and separating said liquid slurry into fiber and a defibered liquid slurry claim 1 , and ...

COMPOSITIONS AND METHODS COMPRISING A XYLANASE ENZYME VARIANT

The present invention provides hemicellulytic enzyme variants. Specifically, the present invention provides xylanase variants having one or more modifications as compared to a parent xylanase enzyme resulting in at least one improved property. In addition, the present invention provides compositions comprising a xylanase variant of the invention. The present invention also provides methods of degrading hemicellulotic material, including arabino-xylan using compositions comprising a xylanase variant of the invention. 2. The xylanase variant according to claim 1 , wherein at least 4 out of 20 possible amino acid substitutions at the productive position makes the xylanase variant able to meet at least one of the criteria a) to c) claim 1 , and wherein the productive position is selected from the group consisting of 135 claim 1 , 28 claim 1 , 57 claim 1 , 62 claim 1 , 70 claim 1 , 79 claim 1 , 89 claim 1 , 102 claim 1 , 105 claim 1 , 118 claim 1 , 151 claim 1 , 153 claim 1 , 160 claim 1 , 181 claim 1 , 184 claim 1 , 200 claim 1 , 220 claim 1 , 232 claim 1 , 262 and 298 claim 1 , 4 claim 1 , 21 claim 1 , 25 claim 1 , 30 claim 1 , 56 claim 1 , 59 claim 1 , 64 claim 1 , 65 claim 1 , 71 claim 1 , 74 claim 1 , 77 claim 1 , 98 claim 1 , 99 claim 1 , 100 claim 1 , 103 claim 1 , 104 claim 1 , 106 claim 1 , 113 claim 1 , 115 claim 1 , 117 claim 1 , 120 claim 1 , 134 claim 1 , 141 claim 1 , 142 claim 1 , 148 claim 1 , 150 claim 1 , 152 claim 1 , 156 claim 1 , 161 claim 1 , 163 claim 1 , 167 claim 1 , 176 claim 1 , 180 claim 1 , 193 claim 1 , 198 claim 1 , 199 claim 1 , 201 claim 1 , 202 claim 1 , 215 claim 1 , 217 claim 1 , 227 claim 1 , 229 claim 1 , 230 and 233 claim 1 , and wherein the polypeptide set forth in SEQ ID NO:2 is used for numbering.3. The xylanase variant according to or claim 1 , wherein at least 8 out of 20 possible amino acid substitutions at the productive position makes the xylanase variant able to meet at least one of the criteria a) to c) claim 1 , wherein ...

METHOD OF MANUFACTURING FERMENTED PORCINE BLOOD AND ANTIBACTERIAL COMPOSITION AND POULTRY FEED COMPOSITION USING SAME

Disclosed are a method of manufacturing fermented porcine blood having antibacterial activity and poultry egg-laying enhancement and weight gain effects, and an antibacterial composition and a poultry feed composition using the fermented porcine blood. 1. A method of manufacturing fermented porcine blood , the method comprising:(a) preparing porcine blood; and{'i': Lactobacillus plantarum, Lactobacillus agilis, Lactobacillus alimentarius, Lactobacillus fermentum', 'Lactobacillus fermentum', 'Saccharomyces cerevisiae, '(b) subjecting the porcine blood to inoculation with a fermentation strain selected from among and a strain mixture of and , and to fermentation culture.'}2. The method of claim 1 , wherein the porcine blood is added with a blood anticoagulant claim 1 , and hydrolyzing the porcine blood with a proteolytic enzyme is performed after step (a) and before step (b) claim 1 , whereby the inoculation with the fermentation strain is carried out for the porcine blood hydrolyzed with the proteolytic enzyme.3. The method of claim 2 , wherein the hydrolyzed porcine blood is added with 5 to 7 wt % of a carbon source claim 2 , and the culture is carried out at 30° C. to 45° C.4. A fermented porcine blood claim 1 , obtained by the method of .5. An antibacterial composition claim 4 , comprising the fermented porcine blood of as an active ingredient.6E. coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosaSalmonella typhimurium.. The composition of claim 5 , which has antibacterial activity against and7. The composition of claim 5 , wherein the composition is a quasi-drug composition.8. The composition of claim 5 , wherein the composition is a pharmaceutical composition.9. The composition of claim 5 , wherein the composition is a cosmetic composition.10. The composition of claim 5 , wherein the composition is a food composition.11. The composition of claim 5 , wherein the composition is a feed composition.12. A poultry feed composition for egg- ...

SYNTHESIS OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS BY RECOMBINANT CELL

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids. 1221-. (canceled)222. A recombinant nucleic acid molecule comprisinga polynucleotide which encodes a Δ5/Δ6 bifunctional desaturase which catalyses desaturation of α-linolenic acid (ALA) to stearidonic acid (SDA) and desaturation of eicosatetraenoic acid (ETA) to eicosapentaenoic acid (EPA); anda polynucleotide which encodes a Δ6 elongase which catalyses elongation of SDA to ETA;wherein each polynucleotide is operably linked to a promoter which directs expression of the polynucleotide in a plant seed.223. The recombinant nucleic acid molecule of claim 222 , wherein the Δ5/Δ6 bifunctional desaturase catalyses desaturation of an acyl-CoA substrate.224. The recombinant nucleic acid molecule of claim 223 , wherein the Δ6 elongase is a Δ5/Δ6 bifunctional elongase.225Brassica napusArabidopsis thaliana. The recombinant nucleic acid molecule of claim 222 , wherein the plant seed is a or seed.226. A cell comprising the recombinant nucleic acid molecule of .227. A cell comprising the recombinant nucleic acid molecule of .228. The cell of which is a plant cell.229. The cell of claim 228 , wherein the cell comprises an exogenous polynucleotide which encodes a Δ5 elongase which catalyses elongation of EPA to docosapentaenoic acid (DPA) claim 228 , wherein the polynucleotide is operably linked to a promoter which directs expression of the polynucleotide in the plant seed.230. The cell of claim 229 , wherein the cell comprises an exogenous polynucleotide which ...

BACILLUS SP. STRAIN WITH IMPROVED PRODUCTIVITY OF FERMENTED SOYBEAN MEAL AND METHOD FOR PRODUCING FERMENTED SOYBEAN MEAL USING THE SAME

The present invention relates to a K2G strain, which is excellent in removal of anti-nutritional factors and in protease activity, and shows excellent antimicrobial activity against pathogens and reduced productivity of viscous substances, a method for producing a fermented soybean meal using the strain, a fermented soybean meal produced therefrom, and a feed composition including the same. The fermented soybean meal prepared by K2G strain according to the present invention has few anti-nutritional factors such as trypsin inhibitors, soybean oligosaccharides, and polysaccharides, a high content of crude proteins, and high protein solubility, and also consists of small-sized peptides digestible by livestock due to low-molecularization, thereby being effectively used as a high-quality vegetable protein feed having excellent absorption rate and feed efficiency. 111-. (canceled)12Bacillus amyloliquefaciens. A strain K2G (Korean Culture Center of Microorganisms Accession No. KCCM11471P) for producing a fermented soybean meal by solid state fermentation.13Bacillus amyloliquefaciensBacillus amyloliquefaciens. The strain K2G according to claim 12 , wherein the strain K2G has anti-nutritional factors-removing activity claim 12 , protease activity claim 12 , antimicrobial activity and reduced productivity of viscous substances during fermentation.14Bacillus amyloliquefaciens. A composition comprising the strain K2G (Korean Culture Center of Microorganisms Accession No. KCCM11471P) for producing a fermented soybean meal by solid state fermentation. The present invention relates to a novel sp. strain with improved productivity of fermented soybean meal and a method for producing a fermented soybean meal using the same. More particularly, the present invention relates to a novel strain with improved productivity of fermented soybean meal, which is excellent in removal of anti-nutritional factors and in protease activity, and shows a high antimicrobial activity against pathogens ...

METHOD OF AND SYSTEM FOR PRODUCING A HIGH VALUE ANIMAL FEED ADDITIVE FROM A STILLAGE IN AN ALCOHOL PRODUCTION PROCESS

A method of and a system for controlling the culture growth of high value feed organisms in a co-fermented system that produces ethanol and for the culture to consume both nonprotein and carbohydrate based products. The method and system produce products that enhance the nutritional value of the co-products. The method further provides a system to grow unique value added co-products, including a mycotoxin-free animal feed, for the monogastric and ruminant animal feed markets including aquaculture, poultry, swine, companion animals and livestock animals. The method includes the production of organic acids to enrich animal feed for enhanced animal performance with reduced antibiotic components in the feed. The method includes the production and incorporation of probiotics in the animal feed product. 1. A method of producing a nutrient added animal feed comprising:a. performing a first fermentation; andb. generating an organic acid, probiotics, antioxidants, carotenoids, amino acids or a combination thereof in a second fermentation.2. The method of claim 1 , further comprising adding microorganisms to the second fermentation.3LactobacillusBifidobacteriumStreptococcus. The method of claim 1 , wherein the microorganisms comprise spp. claim 1 , spp. claim 1 , spp. claim 1 , or a combination thereof.4BacillusEnterococcus. The method of claim 1 , wherein the microorganisms comprise spp. claim 1 , spp. claim 1 , or a combination thereof.5. The method of claim 1 , wherein the microorganisms produces proteases breaking emulsion to improve oil recovery.6Bacillus fastidiosus, Aspergillus funiculosus. The method of claim 5 , wherein the microorganisms comprise claim 5 , or a combination thereof.7. The method of claim 1 , further comprising providing an environment suitable for a growth of probiotic microorganisms.8. The method of claim 1 , wherein the organic acid comprises lactic acid or acetic acid.9. The method of claim 1 , further comprising growing probiotics using a liquid ...

METHOD FOR CONCENTRATING PROTEIN IN GRAIN POWDER

Provided are a method of concentrating protein in grain powder, grain powder including protein that has been concentrated by using the method, and a feed additive including the grain powder including concentrated protein. According to the method of concentrating protein in grain powder, grain powder is treated with enzyme to increase the water-soluble saccharide content in a source, and by inoculating bacteria or yeast and fermentation, the increased water-soluble saccharide is removed, leading to a higher concentration of protein. Thus, the protein content ratio increase effects and the function of grain powder as a protein source are enhanced. 1. A method of concentrating protein in grain powder , the method comprising:treating grain powder with enzyme to decompose structural carbohydrate; andinoculating bacteria into the grain powder to perform fermentation.2. The method of claim 1 , further comprising claim 1 , prior to the treating with enzyme claim 1 , adding a base solution to the grain powder to adjust a pH that is optimal for the growth of the bacteria in the grain powder.3Bacillus.. The method of claim 1 , wherein the bacteria are genus4BacillusBacillus subtilis, Bacillus licheniformis, Bacillus toyoi, Bacillus coagulans, Bacillus polyfermenticusBacillus amyloliquefaciens. The method of claim 3 , wherein the genus comprises at least one strain selected from the group consisting of claim 3 , and K2G.5. The method of claim 1 , wherein the bacteria are lactic acid bacteria.6LactobacillusLactococcusLeuconostocPediococcusBifidobacterium.. The method of claim 5 , wherein the lactic acid bacteria comprise at least one strain selected from the group consisting of genus claim 5 , genus claim 5 , genus claim 5 , genus claim 5 , and genus7. A method of concentrating protein in grain powder claim 5 , the method comprising:treating grain powder with enzyme to decompose structural carbohydrate; andinoculating yeast into the grain powder to perform fermentation. ...

METHOD FOR PREPARING FERMENTED COMPOSITION WITH IMPROVED ODOR USING YEAST

The present disclosure relates to a method for preparing a fermented composition, and more specifically, to a method for preparing a fermented composition with improved odor, which comprises preparing grain flour; performing primary fermentation of the grain flour using yeast; performing secondary fermentation of the primary fermented product using a strain of the genus ; and obtaining the secondary fermented product. The fermented composition of the present disclosure has a high content of a peptide with a low molecular weight, and thus enables the increase of digestibility and absorption rate of proteins during ingestion while also improving the peculiar odor of a fermented product to enhance its palatability. 1: A method for preparing a fermented composition with improved odor , comprising:preparing grain flour;performing primary fermentation of the grain flour using yeast;{'i': 'Bacillus', 'performing secondary fermentation of the primary fermented product using a strain of the genus ; and'}obtaining the secondary fermented product.2: The method according to claim 1 , wherein the yeast produces α-galactosidase claim 1 , protease claim 1 , and phytase.3Saccharomyces cerevisiae: The method according to claim 1 , wherein the yeast is deposited under Accession Number KCCM12123P or Accession Number KCCM12124P.4: The method according to claim 1 , wherein claim 1 , in the secondary fermented product claim 1 , a peptide with a molecular weight of 30 kDa or less is contained in an amount of 40% to 100%.5: The method according to claim 1 , wherein the grain flour comprises soybean meal or corn gluten.6: The method according to claim 1 , wherein the performing of the primary fermentation of the grain flour comprises adding α-amylase or glucoamylase.7: The method according to claim 1 , wherein the grain flour undergoes moisture content adjustment and then heat treatment.8: The method according to claim 7 , wherein the adjusted moisture content is in a range of 30% to 60%. ...

一种发酵饲料及其制备方法

本发明公开了一种发酵饲料及其制备方法，其中，所述发酵饲料包括以下重量百分比的原料：氨基葡萄糖发酵后的残余混合物19‑25%、玉米芯23‑25%、豆粕粉28‑31%、秸秆12‑20%、餐厨废弃物7‑9%；上述原料的制备方法如下：（1）称取上述各原料，备用；（2）将餐厨废弃物捣碎，然后与氨基葡萄糖发酵后的残余混合物、玉米芯、豆粕和秸秆混合发酵，得到所述发酵饲料。本发明中采用氨基葡萄糖发酵后的残余混合物和餐厨废弃物，将废物利用，变废为宝，提高了资源的利用率；发酵得到的饲料中的营养物质符合发酵质量标准，能够应用到畜禽等饲料中。

用于生产可食用的真菌菌丝体共混肉和肉类似物组合物的增强的好氧发酵方法

本文提供了耐贮藏的蛋白质食品成分、包含耐贮藏的蛋白质食品成分的食品产品、它们的生产方法以及它们的使用方法。耐贮藏的蛋白质食品成分包含经培养的真菌生物质和有限量的水。有利地，可以在食品供应中存储、运输和递送耐贮藏的蛋白质食品成分。

Method and product (versions) of processing suspension of plankton chlorella strains, algolised compound food, method of obtaining thereof, method of introducing product of processing into system of watering birds or pigs

FIELD: chemistry. SUBSTANCE: invention relates to biotechnology, namely to method of processing suspension of plankton strains of Chlorella. Method includes electrofloatation, separation of formed foam together with small amount of suspension or culture medium, sufficient for providing cell density from 30 to 1000 mln/ml in obtained Chlorella biomass concentrate, with obtaining Chlorella biomass concentrate and purified culture medium. Culture medium is concentrated. Method makes it possible to obtain products in form of Chlorella biomass concentrate, culture medium concentrate and their mixture. Method of granulated compound food algolisation includes introduction of product obtained by processing suspension of plankton Chlorella strains with density of Chlorella cells from 50 to 500 mln/ml into compound food either before granulation into mixer or directly in the process of granulation into granulator in amount 2-3 wt %. Method of direct supply of Chlorella concentrate into system of watering birds or pigs includes dosed supply of concentrate via medicator in amount, providing density of Chlorella cells in water 10-50 mln/ml. EFFECT: realisation of invention provides increased efficiency of Chlorella processing, namely ensures reduction of expenditures and increased manufacturability of application of products obtained as a result of Chlorella processing. 10 cl, 10 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 538 399 C2 (51) МПК A23K 1/00 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ (21)(22) Заявка: ИЗОБРЕТЕНИЯ К ПАТЕНТУ 2013104225/13, 01.02.2013 (24) Дата начала отсчета срока действия патента: 01.02.2013 (72) Автор(ы): Куницын Михаил Владиславович (RU) (73) Патентообладатель(и): Куницын Михаил Владиславович (RU) (43) Дата публикации заявки: 10.08.2014 Бюл. № 22 R U Приоритет(ы): (22) Дата подачи заявки: 01.02.2013 (45) Опубликовано: 10.01.2015 Бюл. № 1 2 5 3 8 3 9 9 R U C 2 C 2 A1, 30.12.1976. SU 1430021 A1, 15.10.1988. КАПУСТИН Н.К ...

Supplements for combined feed-stuff based on fermentative broth and method of their production by way of granulation

FIELD: food industry. ^ SUBSTANCE: group of inventions relates to animal feeding and involves a method for production of a supplement of fermentative broth containing L- lysine and a supplement for animals produced by this method. The supplement production method envisages the following stages: granules formation immediately of L- lysine fermentative broth without passing through the filtration process, the granules weight amount equal to nearly 48-52%; mixing the concentrate with an active agent regulating purity or with free lysine for formation of a mixture concentrate with corrected L- lysine content; formation of granules with bulbous structure from the concentrate mixture, cooling the prepared granules with the granules formation stage thus completed; inoculums placement into the chamber, upward directed air injection into the chamber from under the inoculums so that minimum quantity of the granules is floated in the chamber, spraying the mixture concentrate at the moment of air injection whereby the concentrate mixture sprayed onto the granules is dried by injected air and further removal of finished particles from the chamber. ^ EFFECT: group of inventions ensures production of a supplement for animals with lysine-HCI purity at least 63%, the maximum water content is 3% and pour density is 67050 kg/m3. ^ 18 cl, 7 dwg, 3 tbl, 4 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) 2 415 601 (13) C2 (51) МПК A23K A23K 1/00 1/16 (2006.01) (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ, ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (21)(22) Заявка: 2008126919/13, 10.01.2007 (24) Дата начала отсчета срока действия патента: 10.01.2007 (43) Дата публикации заявки: 20.02.2010 Бюл. № 5 (56) Список документов, цитированных в отчете о поиске: KR 20050097678 A, 10.10.2005. KR 20050085618 A, 29.08.2005. US 5622710 A, 22.04.1997. (73) Патентообладатель(и): СИ-ДЖЕЙ ЧЕИЛДЖЕДАНГ КОРПОРЕЙШН (KR) 2 4 1 5 6 0 1 R U (86) Заявка PCT: KR 2007/000188 (10.01.2007) C ...

Biochemical method for obtaining of powdered food product and powdered food product

FIELD: biotechnology. SUBSTANCE: method involves obtaining product having fermentative properties by providing autolysis of wheat perisperm and wheat sprout in cultural bouillon, such as bacteria or mold; mixing with methionine and soda salt of native deoxyribonucleic acid extracted from plant or animal substances or microorganisms; drying resultant mixture at temperature of 60 C and grinding. Method provides obtaining of food product comprising wheat perisperm and wheat sprout autolisate, soda salt of deoxyribonucleic acid-methionine with excipient additive. Food product is enriched in autolysis products - amino acids, main fatty acids, and vitamins providing biological and nutritive value of product. EFFECT: increased efficiency and improved quality of food product. 7 cl, 3 ex ОоОсэ9гсс ПЧ сэ (19) РОССИЙСКОЕ АГЕНТСТВО ПО ПАТЕНТАМ И ТОВАРНЫМ ЗНАКАМ ВИ” 2 216 201 ^^ С2 (51) МПК” д 23 р 1/03, А 23 К 11/165, А 23 4 1/18 12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ РОССИЙСКОЙ ФЕДЕРАЦИИ (21), (22) Заявка: 98117085/13, 11.02.1997 (24) Дата начала действия патента: 11.02.1997 (30) Приоритет: 12.02.1996 СН 349/96 (43) Дата публикации заявки: 21.08.2000 (46) Дата публикации: 20.11.2003 (56) Ссылки: ОЕ 1792630 А, 09.03.1972. СВ 1151236 А, 07.05.1969. КУ 2048123 СЛ, 20.11.1995. (85) Дата перевода заявки РСТ на национальную фазу: 14.09.1998 (86) Заявка РСТ: 1В 97/00105 (11.02.1997) (87) Публикация РСТ: М/О 97/28699 (14.08.1997) (98) Адрес для переписки: 129010, Москва, ул. Б.Спасская, 25, стр.3, ООО "Юридическая фирма Городисский и Партнеры", пат.пов. Н.Г.Лебедевой, рег.№ 0112 (71) Заявитель: МЕНА Аннализа (1Т) (72) Изобретатель: ДЖЬЮФФРЕ ИЗЛЕР Лаура (СН) (73) Патентообладатель: МЕНА Аннализа (1Т) (74) Патентный поверенный: Лебедева Наталья Георгиевна (54) БИОХИМИЧЕСКИЙ СПОСОБ ПОЛУЧЕНИЯ ПОРОШКООБРАЗНОГО ПИЩЕВОГО ПРОДУКТА И ПОРОШКООБРАЗНЫЙ ПИЩЕВОЙ ПРОДУКТ (57) Изобретение относится к биотехнологии. Способ получения — порошкообразного пищевого продукта для людей включает получение ...

具有抗氧化能力的乳酸菌菌株的筛选方法及应用

本发明公开了一株具有抗氧化能力的乳酸菌菌株的筛选方法及应用。所述戊糖片球菌ZJUFA‑4，保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏号为CGMCC NO：16767。该菌株从传统腌菜中分离得到，经过过氧化氢耐受能力初筛，菌株DPPH自由基、羟自由基以及超氧阴离子自由基清除能力复筛得到。利用该菌株发酵玉米豆粕复合饲料，提高发酵饲料抗氧化酶活性以及抗氧化物质含量，用于提高育肥猪生长性能和抗氧化水平。

Method of production of protein-vitamin additive of starch-containing grain raw material

FIELD: agriculture. SUBSTANCE: invention relates to agriculture, namely to fodder production, and can be used in production of fodder yeast from hydrolysates, obtained during processing of vegetable raw material. Method includes preliminary processing of grain starch-containing material, preparation of nutrient medium consisting of carbohydrate and salt parts, cultivation of yeast culture on prepared nutrient medium in fermenter in continuous mode, further concentration and drying of yeast suspension to obtain end product. Preliminary processing of grain starch-containing material involves its crushing by wet method, separation of grain shells, extraction of gluten, serial enzymatic hydrolysis of remaining starch part with alpha-amylase and glucoamylase to obtain product with glucose content no less than 25 %, enzymatic hydrolysis with cellulolytic enzyme preparations containing xylonase and cellulase, separation of grain shells after preliminary thermo-chemical treatment to obtain product with glucose content of no less than 12 %. Obtained glucose-containing parts of nutrient medium are combined with mineral part of nutrient medium and successive culturing of yeast Hansenula polymorpha genus VKPM Y-314 in fermenter is performed in continuous mode Hansenula jadinii VKPM Y-797, Hansenula petersonii VKPM Y-1012 with subsequent concentration of yeast biomass, which is dried and granulated to obtain end product. Wherein initial and running medium for cultivation of second and further cultures of yeast is nutrient medium filtrate from previous fermentation process. For yeast Hansenula polymorpha VKPM Y-314 nutrient medium with content of reducing substances no less than 20 % is supplied. Recovered gluten, after additional cleaning, is released in form of ready product – fodder gluten with protein content no less than 70 %. Invention implementation provides production of protein-vitamin additive with high content of proteins, vitamins of group B and indispensable amino ...

Process for by product ferment lysine

본 발명은 라이신 발효에서 발생하는 발효부산액을 처리하는 방법에 관한 것으로, 보다 상세하게는 발효부산액에 과량 존재하는 황산염, 즉 유안과 황산칼륨의 용해도를 이용한 농축법으로 분리하면서 부산액의 농도를 50%(W/W)에서 70%(W/W)로 농축하여 가축의 사료원으로 사용하는 방법에 관한 것이다. The present invention relates to a method for treating fermentation by-products arising from lysine fermentation, and more particularly, to the concentration of by-products while separating by the concentration method using the solubility of sulfate, that is, yuan and potassium sulfate, present in the fermentation by-products. To a concentration of 50% (W / W) to 70% (W / W) to use as a feed source for livestock. 따라서, 본발명의 목적은 라이신 발효부산액에 과량존재하는 무기염을 분리, 농축하여 가축의 사료원으로 사용하는 방법을 제공함에 있다. Accordingly, an object of the present invention is to provide a method for separating and concentrating an inorganic salt in excess of lysine fermentation by-product and using it as a feed source for livestock. 즉, 본 발명은 라이신 발효부산액에 함유되어 있는 황산염의 용해도를 이용한 농축법으로 결정탈수, 분리하여 황함량을 7.5%(W/W)에서 1.5%(W/W)로 낮추고, 발효부산액의 농도를 50%(W/W)에서 70%(W/W)로 농축하여 가축의 사료원으로 사용함을 특징으로 한다. In other words, the present invention is dehydrated and separated by the concentration method using the solubility of the sulfate contained in the lysine fermentation by-products to lower the sulfur content from 7.5% (W / W) to 1.5% (W / W), fermentation by-products The concentration of 50% (W / W) to 70% (W / W) is characterized by using as a feed source for livestock. 본 발명에 의한 황산염이 제거된 라이신 발효부산물은 가축 사료원으로 사용할 수 있다. Lysine fermentation by-product from which the sulfate is removed according to the present invention can be used as a livestock feed source.

Method of food product obtaining

FIELD: agriculture. SUBSTANCE: invention relates to agriculture, specifically to fodder production. Method for production of a grain feedstock based feed product is implemented by preparing a nutrient substrate, introducing a producer culture, culturing the microorganisms and drying the resulting mixture. Nutrient substrate is the curative waters of corn grain or a mixture of corn wax and wet extruded cornmeal in a ratio of 1:3–1:10. As the producer culture, the Trichosporon pullulans yeast strain is used, able to actively grow at pH 4.0–8.5 and alkalize the acidic substrate during growth. EFFECT: implementation of the invention makes it possible, using cheap, not requiring special preparation techniques, difficult to dispose of byproducts of starch-fiber production, to obtain a fodder product with high biological value. 1 cl, 3 ex РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 646 047 C1 (51) МПК A23K 10/12 (2016.01) A23K 10/18 (2016.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (52) СПК A23K 10/12 (2006.01); A23K 10/18 (2006.01) (21)(22) Заявка: 2016152219, 29.12.2016 (24) Дата начала отсчета срока действия патента: Дата регистрации: 01.03.2018 (45) Опубликовано: 01.03.2018 Бюл. № 7 2220590 C1, 10.01.2004. US 20160249638 A1, 01.09.2016. US 5354818 A, 11.10.1994. (54) СПОСОБ ПОЛУЧЕНИЯ КОРМОВОГО ПРОДУКТА (57) Реферат: Изобретение относится к сельскому хозяйству, продуцента используют штамм дрожжей а именно к кормопроизводству. Способ Trichosporon pullulans, способный активно расти получения кормового продукта на основе при рН 4.0-8.5 и подщелачивать в процессе роста зернового сырья осуществляют путем подготовки кислый субстрат. Осуществление изобретения питательного субстрата, внесения культурыпозволяет, используя дешёвые, не требующие продуцента, культивирования микроорганизмов специальных приёмов подготовки, трудно и высушивания полученной смеси. Питательным утилизируемые побочные продукты субстратом служат замочные воды ...

Bacillus sp. strain with improved productivity of fermented soybean meal and method for producing fermented soybean meal using the same

The present invention relates to a Bacillus amyloliquefaciens K2G strain, which has a superior activity for removing anti-nutritional factors, a superior protease activity, a superior antimicrobial activity against pathogens, a reduced productive capacity of mucilage, a method for producing a fermented soybean meal using the same, a fermented soybean meal produced by the same, and a feed composition containing the same. According to the present invention, the fermented soybean meal which is produced by using a Bacillus amyloliquefaciens K2G strain is almost lost of various anti-nutritional factors such as a trypsin inhibitor, soybean oligosaccharides and polysaccharides, etc.; has a high crude protein content, and a high protein solubility; and is composed of small-sized peptides which can be easily digested absorbed by livestock. Accordingly, the present invention can be usefully used as a high-quality vegetable protein feed, which has a superior absorption rate and feed efficiency.

Bacillus strain having improved productivity of fermented soybean meal and method for producing fermented soybean meal using the same