Transgenic maize with enhanced phenotype
(19) AUSTRALIAN PATENT OFFICE (54) Title Transgenic maize with enhanced phenotype (51)6 International Patent Classification(s) C12N 015/82 C12N 015/87 (21) Application No: 2002351325 (22) Applic (87) WIPO No: WO04/053055 (30) Priority Data (31) Number (32) Date (33) Country 60/337,358 2002.12.04 US (43) Publication Date : 2004 .06.30 (43) Publication Journal Date : 2004 .08.05 (54) Title Transgenic maize with enhanced phenotype (51)6 International Patent Classification(s) C12N 015/82 C12N 015/87 (21) Application No: 2002351325 (22) Applic (87) WIPO No: WO04/053055 (30) Priority Data (31) Number (32) Date (33) Country 60/337,358 2002.12.04 US (43) Publication Date : 2004 .06.30 (43) Publication Journal Date : 2004 .08.05 2002.12.04 (71) Applicant(s) BODDUPALLI, Raghava; MONSANTO TECHNOLOGY BANU, G.; DENG, Molian; DONG, Jinzhuo; DALY, LLC; BELL, Erin; KRETZMER, Keith, A.; Mackenzie; DEIKMAN, Jill (72) Inventor(s) Jung, Vincent; Daly, Mackenzie; Malloy, Kathleen; Michael ; Xu, Nanfei; Deikman, Jill; Xin, Zhanguo; Philip W.; Boddupalli, Raghava; Lai, Chao Qiang; G. ; Johnson, Richard G.; Parnell, Laurence; Ruff, Ahrens, Jeffrey; Zhou, Li; Luethy, Lin, Jie-Yi; Dong, Jinzhuo; Miller, Agarwal, Ameeta K.; Start, William Thomas G.; Tennesen, Dan; Vidya, K.R. ; Laccetti, Lucille B.; Adams, Thomas H.; Huang, Shihshieh; Madson, Linda L.; Banu, G. ; Bell, Erin; Liu, Jingdong; Hinchey, Brendan S.; Chomet, Paul S.; Zhang, Qiang; Yang, Chunzi ; Duff, Stephen M.; Edgerton, Michael D.; Lee, Garrett J.; Ball, James A.; Padmathi, Machikanti; Lu, Bin; Zeng, Xiaoping; Wang, Haiyun; Zhao, Yajuan; Lund, Adrian; Deng, Molian; Kretzmer, Keith A.; Mckiel, Christine L.; Galligan, Meghan -1- Transgenic maize with an enhanced phenotype from insertion of heterologous DNA. Enhanced phenotypes include yield such as yield under stress conditions, quality of plant morphology, physiology or seed composition, metabolic function, cell growth, and the like. Such transgenic maize is produced by generating a plurality of transgenic events for a plurality of unique transgenic DNA constructs where each of the transgenic events comprises introducing into the genome of a parental maize line a single transgenic DNA construct comprising a promoter operably linked to heterologous DNA in sufficient quantity to produce transgenic cells which can be cultured into plants of transgenic maize having the enhanced phenotype. What is claimed is: 1. Transgenic maize seed characterized by enhanced yield (as measured in weight of crop per area) as compared to a corresponding yield of its parental maize line, obtained by introduction into the genome of said parental line of a transgenic DNA construct comprising a promoter operably linked to heterologous DNA, wherein said heterologous DNA encodes a protein having an amino acid sequence which is at least 60% identical to a sequence selected from the group consisting of SEQ ID NO : 368 through SEQ ID NO : 736.
2. Transgenic maize seed according to claim wherein said heterologous DNA comprises a protein coding segment of DNA having at least 60% identity with a sequence selected from the group consisting of SEQ ID NO : 1 through SEQ ID NO: 368.
3. Transgenic maize seed according to claim wherein said enhanced yield is the result of improved plant growth under one or more stress conditions in the group consisting of drought, shade, fungal disease, viral disease, bacterial disease, insect infestation, nematode infestation, cold temperature exposure, heat exposure, osmotic stress, reduced nitrogen nutrient availability, reduced phosphorus nutrient availability and high plant density.
4. Transgenic maize seed according to claim wherein said enhanced yield is also manifested by increase in number of kernels per ear, number of ears per unit planted area, or average weight of kernels.
5. Transgenic maize seed according to claim wherein said enhanced yield is also manifested by increase in biomass per unit planted area or an increase in the root/shoot ratio.
6. Transgenic maize seed according to claim wherein said enhanced yield is also manifested by increased efficiency by said transgenic plant in water use, nitrogen use or phosphorus use.
7. Transgenic maize seed characterized by enhanced quality in the plant morphology, physiology or seed as compared to a corresponding phenotype of a parental maize line, obtained by introduction into the genome of said parental line a transgenic DNA construct comprising a promoter operably linked to heterologous DNA, wherein said heterologous DNA encodes a protein having an amino acid sequence which is at least 80% identical to a sequence selected from the group consisting of SEQ ID NO : 369 through SEQ ID NO : 738. <Desc/Clms Page number 48> 8. Transgenic maize seed according to claim 7 wherein said heterologous DNA comprises a protein coding segment of DNA having at least 70% identity with a sequence selected from the group consisting of SEQ ID NO : 1 through SEQ ID NO: 368.
9. Transgenic maize seed according to claim 7 wherein said enhanced quality is shortened internode length, increased internode length, early leaf senescence, sterility, elongated tassel central axis, setting a second ear at high planting density, earlier time of germination, increased production of kernel oil or increased production of kernel protein.
10. Transgenic maize seed according to claim 9 having an enhanced phenotype of decreased internode length resulting from introduction of heterologous DNA coding for: (d) a TOCl-like receiver domain 3 having an amino acid sequence which is at least 60% identical to SEQ ID NO : 436, (e) a HY5-like protein having an amino acid sequence which is at least 60% identical to SEQ ID NO : 565, or (f) a proline permease having an amino acid sequence which is at least 60% identical to SEQ ID NO : 371.
11. Transgenic maize seed according to claim 9 having an enhanced phenotype of increased internode length resulting from introduction of heterologous DNA coding for: (c) a myb transcription factor having an amino acid sequence which is at least 60% identical to SEQ ID NO : 717, or (d) an SVP-lilce protein having an amino acid sequence which is at least 60% identical to SEQ ID NO: 609.
12. Transgenic maize seed according to claim 9 having an enhanced phenotype of early leaf senescence resulting from insertion of heterologous DNA coding for a Cytochrome P450 having an amino acid sequence which is at least 60% identical to SEQ ID NO : 382.
13. Transgenic maize seed according to claim 9 having an enhanced phenotype of sterility resulting from insertion of heterologous DNA coding for: (e) an RR3-like receiver domain 8 having an amino acid sequence which is at least 60% identical to SEQ ID NO : 439, (f) an ARR2-like receiver domain having an amino acid sequence which is at least 60% identical to SEQ ID NO : 434, <Desc/Clms Page number 49> (g) an HSF protein having an amino acid sequence which is at least 60% identical to SEQ ID NO : 487, or (h) an SVP-like protein having an amino acid sequence which is at least 60% identical to SEQ ID NO : 609.
14. Transgenic maize seed according to claim 9 having an enhanced phenotype of elongated tassel central axis resulting from insertion of heterologous DNA coding for an SVP-like protein having an amino acid sequence which is at least 60% identical to SEQ ID NO : 609.
15. A method for introducing into a maize line an enhanced phenotype as compared to a phenotype in parental units of said maize line, said method comprising (a) generating a population of transgenic plants comprising a variety of heterologous DNA for the transcription of which there is no known phenotype in corn, (b) observing phenotypes for said transgenic plants, (c) selecting seeds from transgenic plants having an unexpected enhanced phenotype, and (d) optionally, repeating a cycle of germinating transgenic seed, growing subsequent generation plants from said transgenic seed, observing phenotypes of said subsequent generation plants and collecting seeds from subsequent generation plants having an enhanced phenotype.
16. A method according to claim 15 wherein said population of transgenic plants is produced by generating a plurality of transgenic events for a plurality of unique transgenic DNA constructs wherein each of said transgenic events comprises introducing into the genome of a parental maize line a single transgenic DNA construct comprising a promoter operably linked to heterologous DNA, wherein said transgenic DNA construct is introduced into said genome in sufficient quantity to produce transgenic cells which can be cultured into plants of transgenic maize comprising said population. culturing said transgenic cells into a population of transgenic plants producing progeny transgenic seed, 17. A method according to claim 16 wherein said plurality of transgenic events is at least 2 and said plurality of unique transgenic DNA constructs is at least 20.
18. A method according to claim 16 wherein said plurality of transgenic events is at least 2 and said plurality of unique transgenic DNA constructs is at least 50. <Desc/Clms Page number 50> 19. A method according to claim 16 wherein said DNA construct comprises heterologous DNA operably linked to the 5'end of a promoter region comprising a rice actin promoter and rice actin intron.
20. A method according to claim 16 further comprising crossing transgenic plants from said population with at least one other maize line to produce a hybrid population, observing phenotypes in said hybrid population and selecting seed from plants having an unexpected phenotype.