NICOTIANA-SPECIFIC MOLECULAR MARKER FOR DISCRIMINATING TOBACCO FROM NON-TOBACCO AND APPLICATION THEREOF

The present invention belongs to the field of molecular biotechnology. More specifically, it relates to a Nicotiana-specific molecular marker for discriminating tobacco from non-tobacco and application thereof. The numbers of the Nicotiana-specific molecular marker comprise Ntsp027 and Ntsp151, and the nucleotide sequences of the PCR amplified products thereof are shown in SEQ ID No .1 and SEQ ID No .2, respectively. The application is to use the Nicotiana-specific molecular markers as described above to perform PCR amplification on the genomic DNA of the tobacco raw material involved to detect whether there is a specific nucleotide sequence of the PCR amplified product, and to accurately determine whether tobacco materials are present in the tobacco raw material involved. The Nicotiana-specific molecular marker of the present invention realizes the rapid and effective identification and detection of tobacco and non-tobacco, improves the scientificity, accuracy and authority of the identification and detection of the tobacco raw material involved, reduces judicial risks, and provides strong judicial evidence for the anti-counterfeiting and anti-smuggling in the tobacco industry monopoly.