UDP-glucose-4-epimerase useful for improving agronomic performance of plants

1. An isolated polynucleotide, comprising a nucleotide sequence selected from the following:

(A)   nucleotide sequence, its coding with UDP-glucose epimerase activity polypeptide, wherein the polypeptide has the   ID   NO SEQ:   1 compared with at least 70% amino acid sequence of sequence identity, based on the Clustal   V comparison method, wherein the PV to the comparison of the default parameter: KTUPLE= 1, GAP   PENALTY= 3, WINDOW = 5 and DIAGONALS   SAVED= 5;

(B)   a nucleotide sequence, its coding with UDP-glucose epimerase activity, wherein said nucleotide sequences under the stringent conditions comprising SEQ   NO   ID and:   18 of the integrity of the molecular hybridization DNA complement each other;

(C)   nucleotide sequence, its coding with UDP-glucose epimerase activity polypeptide, through at least one of them selected from deletion, replacement, add and inserting method, by altering one or more nucleotides, from SEQ   NO   ID:   18 derive said nucleotide sequence;

(D) a nucleotide sequence   encoding a polypeptide, wherein the polypeptide comprising the amino acid sequence   ID   NO SEQ:   1 ; and

(E) containing SEQ   NO   ID:   18 nucleotide sequence.

2. A recombinant DNA construct, which comprises the at least one regulatory element operably connected according to claim 1 wherein the isolated polynucleotide.

3. A method of increasing plant yield, the method comprising: with at least one expression control element operably connected recombinant chloroplast UDP  -glucose epimerase, wherein said epimerase in positioning in the chloroplast.

4. Method according to Claim 3, wherein said recombinant chloroplast UDP  -glucose epimerase comprising a chloroplast transit peptide.

5. Method according to Claim 3, wherein the chloroplast UDP  -glucose epimerase comprising according to claim 1 said nucleotide sequence.

6. A chloroplast in the in-situ increase for single-galactosyl diaoylglycerol (MGDG) biological synthesis of UDP-galactose production method, the method comprising: in at least one regulation under the control of the promoter element, the chloroplast expressed UDP  -glucose epimerase, wherein said epimerase comprising a chloroplast transit peptide.

7. Method according to Claim 6, wherein the chloroplast UDP  -glucose epimerase comprising according to claim 1 said nucleotide sequence.

8. A changing method for plant agronomy characteristic, said method comprising: containing expression according to claim 1 said nucleotide sequence of the UDP-glucose epimerase.

9. Method according to Claim 8, wherein said agronomy characteristic is selected from the group consisting of: increased photosynthetic efficiency, yield and drought tolerance.

10. A change carbon distribution in order to increase the output of the method, the method includes: express UDP-glucose epimerase, the epimerase increase towards the membrane biological synthetic carbon distribution, can be used by this reduction in starch biosynthesis of the amount of carbon.

11. Method according to Claim 10, wherein said epimerase comprising according to claim 1 said nucleotide sequence.

12. A plant, in its contained in the genome of the recombinant DNA construct, said construct comprising with at least one regulatory element operably connected the polynucleotide, wherein the polynucleotide includes according to claim 1 said nucleotide sequence.

13. Plant according to Claim 12, wherein the DNA construct comprising a recombinant compared to the control plant, the plant exhibits increased yields.

14. Plant according to Claim 12, wherein the DNA construct comprising a recombinant compared to the control plant, the plant exhibits drought tolerance.

15. Plant according to Claim 12, wherein the plant is selected from: corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, millet, sugarcane, switchgrass, tobacco, potato and sugar beet.

16. From according to claim 12 wherein the plant production of seed.

17. A plant cell, in its contained in the genome of the recombinant DNA construct, said construct comprising with at least one regulatory element operably connected the polynucleotide, wherein the polynucleotide includes according to claim 1 said nucleotide sequence.

18. A carrier, which comprises according to claim 1 said polynucleotide.

19. For a method of producing transgenic plants, the method comprises: recombinant DNA construct used for transformation of plant cells, the construct comprising the at least one regulatory element operably connected the polynucleotide, wherein the polynucleotide comprises a nucleotide sequence according to Claim 1, and from the transformed plant cell regeneration of transgenic plant.

20. Method according to Claim 19, wherein the plant is selected from: corn, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, millet, sugarcane, switchgrass, tobacco, potato and sugar beet.

21. A method for the photosynthesis efficiency of the plant, the method including: according to Claim 1 expression of recombinant polynucleotide, wherein the polynucleotide is not express the recombinant more compared with the control plants, the increase of the membrane bioreactor.

22. Method according to Claim 21, wherein the plant is selected from: rice, wheat, corn and soybean.

23. Method according to Claim 21, wherein the content of the photosynthetic efficiency will result in an increase of the growth.

24. Method according to Claim 21, wherein the plant exhibits increased grain yield.

25. An oligonucleotide probe or primer, its derived from   NO SEQ   ID:   18, encoding and detecting   NO SEQ   ID:   1 polynucleotide of the expression of the polypeptide.