Preparation method of anti-SARS (severe acute respiratory syndromes) coronavirus surface antibody
The invention discloses a preparation method of an anti-SARS (severe acute respiratory syndromes) coronavirus surface antibody, which specifically comprises the following steps of firstly screening the cells of an SARS coronavirus surface antigen under a requirement for vigorous stage of life activities; performing immunization injection; after immune response is generated, culturing fusion cells with a culture medium; 12 days later, screening positive clone cells, cloning positive cell strains, and screening out stable monoclonal antibody cell strains; putting the stable monoclonal antibody cell strains in a refrigerator overnight, and taking out and closing in an environment at 37 DEG C for 2 hours; adding into a carbonate buffer solution, and drying to obtain the anti-SARS coronavirus surface antibody. The fusion cells are cultured by an HCA process; after immune response, the fusion cells need to be fused under the effect of PRG. The preparation method disclosed by the invention has the beneficial effects that the anti-SARS coronavirus surface antibody can be successfully prepared, the success rate and purity are very high, the operation is relatively simple and convenient, and popularization is facilitated. 1. A countermeasure SARS coronavirus surface antibody preparation, characterized in that the specific steps are as follows: First of all, the anti-SARS coronavirus screening cell surface antigen, in the natural gas are at the peak of life movement; Immunization to it, should be easy produces Miao after then, a culture medium for fusion cell culturing; 12 days later, cell cloning of positive re-selection, and to the positive cell strains for cloning, the stable imab cell screening; It is placed in the refrigerator overnight, the after being taken out 37 degrees Celsius is closed under the environment of 2 hours; By adding carbonate buffer solution, drying can be. 2. A countermeasure SARS coronavirus surface antibody preparation according to Claim 1, characterized in that the fusion cell culture shown in the HCA method. 3. A countermeasure SARS coronavirus surface antibody preparation according to Claim 1, characterized in that after the immune response, the need for the role of the PRG under the fusion.