COSMETIC COMPOSITION, COMPRISING PLANT EXTRACT AS ACTIVE INGREDIENT

The present invention according to the above-mentioned cosmetic composition for plant extract containing hereinafter but, in terms of scientific techniques used the terms and other definition is not available, the invention in person with skill in the art is provided to commonly understand has the meanings, the description of the subject matter of invention to breach of haze description is given of a publicly known function and configuration can be dispensed to each other.

The present invention refers to useful effects of skin, effect of effective ingredient stability and pulling out the, same skin permanently to an interrupt by provision of human body or plant extract including skin care cosmetic composition number under public affairs substrate.

According to the in addition the present invention, effectively to each efficacy according to the percutaneous absorption of a plant extract, to immediately effect, skin can be continuously under public affairs number.

The term used in the present invention, is "anti-aging" skin whitening, skin aging and for preventing or ameliorating, shrinking, reduction, skin care, skin barrier function improving, skin-irritation (true skin), skin cell proliferation and dehydrogenase, antioxidant capability, be employed for promoting collagen synthesis, such as employed for moisturizing skin protection and skin conditions for improving the general outline of mapped all efficacy, effect may be, in the present invention in particular moisturizing performance and antioxidant siberian bustard pertinent data showing of efficacy, but preferred is not limited.

The term used in the present invention, any suitable method "applied" means that the subject skin contacting the composition with the present invention according to which, through corresponding composition intended for absorb into the skin.

The term used in the present invention, applying a composition of the present invention according to all or delay the number of wrinkles are formed in "prevention" RM billion behavior it is, terms used in the present invention, the present invention according to the pleated state loop "improving" is applying a composition advantageously or keys, or strongly than ever the skin elasticity, due to loss of all speeds and then slowing the aging skin resilience of the existing method or means which have advantages contrast effect behavior can be it means a behavior.

The term used in the present invention, "wrinkles" RM, under iron fine line to the skin by an means, by gene cause, reduction of collagen and elastin present in skin dermis, such as can be caused by external environment. In addition "resilient" RM, skin dermis is subject elastin (elastin) resilient elastic fiber composed indicated by said substrate.

The term used in addition in the present invention, the bacteria resistance and ability "antimicrobial" means, action of microorganisms such as bacteria and fungi from to all mechanisms to their big.

The term used in addition in the present invention, the use of "steroidal anti-inflammatory" which means that the resistance, said sign signal transduction in immune-induced inflammation, atopic dermatitis, allergic dermatitis, inflammatory skin diseases, immune disease and asthma can be a the number one are not disclosed.

In addition, the present invention according to extract individual raw materials and plant is formula, leaves, roots, fruit, seeds, flower, stem, such as the contrast is applied to at least one of extraction solvent selected from publicly known conventional method according can be extracted with. I.e., conventional temperature and pressure conditions using conventional extraction solvent, lycopene, ultrasonic extraction, reflux cooling extraction, heat extraction, extraction at room temperature, heating extraction, supercritical fluid extraction is extracted by the method such as but not the limited to. The, said extraction solvent is limited to but not limited to, water, anhydrous or function 1 - 7 carbon atoms of lower alcohols, hexane, chloroform, ethyl ether, acetone, ethyl acetate, butyl acetate, 1, 3 - butylene glycol, 1, 2 - propylene glycol and 1, 3 - propylene glycol so as to act as one or more than one can be selected from the group consisting, preferably water, methanol, ethanol, propanols and butanol such as chosen from the group of a good ancestry. In addition, said extraction solvent extract by fractioning or positive number obtained in each stage all extract, fraction positive number and water, their dilution liquid, including general outline both to a dry matter content of foods or beverages, in some cases, these freezing and drying, vacuum drying, hot air-dried, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying method such as a dried which, dried extract may have further comminuting process. The, when after the extract by further operations such as course during grinding drying and along with, liquid, lyophilized powder be a concentrated solid phase and is constant.

According to one aspect of the present invention cosmetic composition [sop[sop] extract, extract it buys elder brother anger, elder flower extract, extract and magnetic assembly including a composite ion polynucleotide primrose characterized. The, said water-soluble anion polymer and a hydrophobic cationic monomer are ion binding poly ionic complexes that, for the self-assembly by the group stable grain structure formed on the substrate.

According to one aspect of the present invention cosmetic composition [sop[sop] extract, extract it buys elder brother anger, elder flower extract, extract primrose is to check the a combination of steroidal anti-inflammatory, antimicrobial properties or in synergistic effect to, the above-mentioned active ingredient during storage determination of precipitation is generated and long combination of plant extracts, precipitated crystals are re-dissolving them with other frequency different of effective ingredient composite significantly to tame. Hull or, according to the present invention method for effective deposition number as well as determining billion is remarkably improved in each of effective ingredient available degree may be confirmed.

The, solubility of 1% by weight solids content to conventional door number (a predetermined total extract) or more could not be used alone for remarkably improving the available degree and plant extract, plant extract for combinations thereof-term stability by imparting, cosmetic material and excellent suitability to type number using the same number under public affairs substrate.

According to one aspect of the present invention cosmetic composition may include an additional plant extract including, various functional composite can be lower. The, plant extract is preferably but not limited to additional number is one good mint extract, extract the low which it will carry on shoulder, consonant extract, extract the horehound, speed well extract, lemon balm extract and plant extract in NaCl extract be a surface display cathode ray tube.

According to one aspect in addition of the present invention, said water-soluble anion polymer and a hydrophobic cationic monomer complex ionic complex mixing ratio and kind of self-assembly of the group in a variety of shapes can be formed.

Said water-soluble anion polymer for controlling the, gamma - polyglutamic acid, sodium - carboxymethylcellulose, carbomer either or combinations thereof can be. However, the present invention herein are number are not one which, other natural or synthetic water-soluble anion polymer material having suitable moisturization force can be used for the concave disclosed.

In addition, said water-soluble anionic polymer weight average molecular weight of 500,000 to 3,000,000 Da good moisturization ball thread in one aspect has preferably, preferably one having better but not the limited to 1,000,000 to 3,000,000 Da.

Said hydrophobic cationic monomer comprises an party [til[til] ethanol amine, diol may be pos party [til[til] ethanol amine [ley five, d in advance it will be a [su[su] toeparty [til[til] ethanol amine phosphate, phosphate and a dysprosium oxide dipalmitoyl pos party [til[til] ethanol aminethe reel Oh with it risesparty [til[til] ethanol amine, triacetyl tree methyl ammonium bromide, triacetyl tree methyl ammonium chloride, and a method of preparation of protectant chloride either or combinations thereof it will be burnt die limited to but are not correct.

Said hydrophobic cationic monomer poly ionic complexes of hydrophobicity helps increase formation of self-assembled body of ionic complexes poly in aqueous solutions. In addition, negatively charged hydrophobic cationic monomers is human skin by reducing or filled into said electrical repelling water-soluble anion polymer between a lotion can be water-soluble anion polymer has the ability to further enhance the water as well as the ability to readily number public box transdermal penetration of skin and plant extract combination the present invention according to help their efficacy, the expression can be the effect imparting transcendentalism.

According to one aspect of the present invention, said poly ionic complexes ceramide, retinol, cholesterol, 12 to 18 carbon atoms such as a pharmacologically active ingredient selected from unsaturated fatty acids and fatty acid saturation of a fat-soluble poly ionic complexes is formed by self-assembly in aqueous solutions of hydrophobic process capable of containing therein. The above-mentioned grain structure of the suitable application in improving the stability of the dispersion of a pharmacologically active ingredient or can be solubilized.

According to one aspect of the present invention cosmetic composition relative to the weight of the total composition, said [sop[sop] extract 0. 1 to 30% by weight, said it buys elder brother anger extract 0. 1 to 30% by weight, said elder flower extract 0. 1 to 20% by weight, said primrose extract 0. 1 to 20% by weight and said poly ionic complexes 0. 1 to 5% by weight including a may be disclosed.

In addition of the present invention 400 to 700 nm is 70% or more cosmetic composition according to one aspect having light transparency in visible-light transmittance aqueous cosmetic sprayable compositions disclosed. Preferably, according to one aspect of the present invention cosmetic composition visible light transmittance (T_{550 nm} ) Is 70 to 90% can be 0.1, more preferably 80 to 90% but not the limited to one having an.

Furthermore, in the above-mentioned efficacy for imparting additional functionality by adding a synergistic effects, mint extract, extract the low which it will carry on shoulder, consonant extract, extract the horehound, speed well extract, lemon balm extract and least one plant extract in NaCl yarrowia shadow extract 0. 1 to 20% by weight can be further.

Hereinafter, according to one aspect of the present invention the efficacy of cosmetic composition, effect of specifically described as follows.

First, according to one aspect of the present invention cosmetic composition containing antimicrobial activity, has clause salt activity.

Specifically, the present invention according to cosmetic composition mutant (Staphylococcus aureus), Escherichia coli (E. Coli) and Pseudomonas (Pseudomonas aeruginosa) such as recombinant bacteria and Candida albicans (Candida albicans), the wetting ability of an excellent fungus Aspergillus age (Aspergillus niger) exhibit antimicrobial activity other. In particular, e. coli (E. Coli) and Pseudomonas (Pseudomonas aeruginosa) use the antibacterial activity of excels in absorption spectrometry substrate.

In addition, according to one aspect of the present invention cosmetic composition to produce nitric oxide (NO) of cell number billion, excellent can be steroidal anti-inflammatory effect.

Has a known antioxidant extract as well as conventional [sop[sop] steroidal anti-inflammatory effect but, according to the present invention significantly improved contrast when used alone [sop[sop] extract said steroidal anti-inflammatory effect. In particular, according to the present invention in the above-mentioned performance percutaneous absorption efficacy, immediate effect can be sustained effect in highly preferred.

Next, according to one aspect of the present invention cosmetic compositions have a good anti-aging effect. In the present invention, said moisturizing performance and/or antioxidant potency can be represented an improvement in skin care and increases the derivative but all efficacy for improving skin condition, can effect the concave disclosed.

According to one aspect of the present invention removing free radicals and/or active oxygen kind billion by number antioxidant siberian bustard cosmetic composition excels in absorption spectrometry substrate.

In addition of the present invention excellent moisture attached to the cosmetic composition according to one aspect a water-soluble anion polymer cooperating with water as well as for the self-assembly may be very strongly hold the moisture and strongly hold the combined plant extract for the present invention according to strength, anti-aging after percutaneous absorption can be continuously release to maximize efficacy.

Next, according to one aspect of the present invention has excellent skin a soothing cosmetic composition. , the present invention according to cosmetic composition when applied to the skin, as well as relieving carbostyril derivatives for the manufacture and effectively number, to the input of skin conditions and improve sensitivity effects true processed in the substrate.

According to one aspect of the present invention various cosmetic composition said cosmetic composition may further include an addition number outside the engine are disclosed. The, said number is added stabilizing number, emulsion number, number since, moisturizing number, liquid crystal formula of number, pH control number, antimicrobial number, water-soluble polymer, coating number, sequestering number, amino acid, organic amine, polymer emulsion, adjusting pH number, number skin nutrition, anti-oxidation number, anti-oxidation tank number, antiseptic number, selected from one or more aqueous addition number distribution tube; and maintaining current, wax comprises, hydrocarbon oils contain, higher fat oil production, higher alcohol, synthetic ester oil and silicon oil in NaCl added to a planetary transmission for at least one number; or the like is cited.

The, if said number is commonly used in the art aqueous added material does not defined, specific one example include glycerin, deep with it will bloom, [leyn the writing this call, butylene writing this call, pen [thil writing this call, methyl propane d this year, sorbitol, d glycerin, sweeteners, porous, poly butyl [leyn writing this call -10, polyglycerol -3, polyglycerol -4, polyglycerol -6, polyglycerol -10, polyglycerol -20, polyglycerol -40, sorbitol bath -5, sorbitol bath -6, sorbitol bath -20, sorbitol bath -30, bath -40 sorbitol, inositol, maltitol, liver, mannan, mannitol, mannose, lactitol, lactose, dihydroxypropyl PG - writing base nose side, imidazole derivatives and oh octane d this year, fructose, polyglucamine, L polyglucamine, glucose, 1, 2, 6 - hexane this year, writing base three [su methyl -10, writing base three [su methyl -20, lock system [ni glycerin, the blood tree this year which burns, functionalization of the glycerin, tray toll, tree it will knock down and the pro petal which comes, xylitol, for controlling the mote a (EDTA), guar gum, the [khwu [su hour [tu which is, carrageenan, precipitate, gum arabic, pectin, mannan, starch, a thickening, so that a large implies, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, chondroitin sulfuric acid, cloning e it burnt sulfate, glycogen, [hey[hey] troubles sulfate, hyaluronic acid, sodium hyaluronate, coating [...], [khey[khey] coal sulfate, chondroitin, nothing nose [thin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, state [khey[khey] sulfate, locust bean gum, [swuk at the time of roh writing base unit, carotenoid [nin it buys, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, polyvinyl pyrrolidone, carboxy vinyl polymer, sodium polyacrylate, polyethylene glycol, bentonite, para, para profile, lung stipendiary hour ethanol, 1, 2 - hexanediol, such as at least one selected from ethyl [heyk thread glycerin be a 1.

In addition, adding said number is not limited if the material commonly used in the art planetary and, olive oil, camellia seed type, by weight of a jojoba oil, triglyceride, tree octane acid glycerin, tree maintenance this cow it sells the mitt it buys the glycerin liquid such as coconut oil, hardened coconut oil, palm oil, made, for solid hardened castor oil maintaining, on the one hand, candelilla wax, car or an optical sensor wax, lanolin, jojoba wax and the like are exemplified. Hydrocarbon oils whenever include paraffin oil, squalene, petrolatum, micro creasing dephosphorisation wax such as are exemplified. Higher fatty acid include right [lu it buys, the [su the [thu it buys pre, stearic acid and palmitic acid, stearic acid, such as wax comprises hemp cloth [hey it buys, triacetyl alcohol, [su reel biting alcohol, hemp cloth [hey neel alcohol, alcohol pre-steel, can be exemplified such as dibenzothiazyl disulfide [su reel biting alcohol, synthetic ester oil is ISO pro it will bloom stay [thu, triacetyl jade other roh a [thu, the jade [thil it will be burnt stay [thu, ISO pro it will bloom and sells the american reel the [thu, [heyk[heyk] Thread thunder [thu[thu], the steal in advance stay [thu pre, triacetyl lactate, tax [thil this source reel oh [ley [thu isocyanate, neopentylglycol pen [thil glycol d car [phu [thu, ethyl [heyk thread glycerin, triacetyl the ethyl [heyk it buys roh a [thu, ethyl [heyk[heyk] The thread sells the American reel the [thu[thu], dibenzothiazyl disulfide [su reel biting alcohol such as higher alcohols, dimethyl polysiloxane, methyl the phenyl pulley room rock it buys, like methyl the high draw [ceyn[ceyn] the pulley room rock it buys chains of type silicon oil, accumulation of the claw between the car methyl [heyk the factual rock it buys, preparation of cyclosporin methyl cyclotetrasiloxane, such as such as the claw pen between the car methyl the rock which will burn it buys annular silicon oil can be a selected limited to are not correct.

One aspect of the invention the present invention a manufacturing method that is particularly disclosure according to number the elder brother anger in addition, using conventionally known manufacturing method, general emulsifier can be solubilized type and number like in the form of type number number bath the concave disclosed. The, said cosmetic composition enables the desired moderate number can be elder brother anger, specific for example, flexible skin, nutritional skin water, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder such as elder brother anger-number number but not the limited to a target area.

Hereinafter, the present invention in the embodiment for producing by more specifically described as follows. In the embodiment according to the present invention these is to aid in the hell of, the meaning of the present invention these range number by any one with it held and not the.

(Evaluation method)

1. Storage-stable evaluated

In cosmetic composition prepared by the number in the embodiment and comparison to 20 g each content amount is placed in a vial, room temperature (25 ± 2 °C), cold thermostat (4 ± 2 °C), high temperature thermostat (45 ± 2 °C) (Freeze provided Thaw Chamber, 45 °C -20 °C 1 1 times in a period of circulation) and cycle chamber storage amount, 1 weeks intervals determining inspection method and a naked eye of each sample precipitation observed.

2. Antimicrobial test

In a cosmetic composition for measuring antimicrobial activity in the embodiment and comparison number produced therewith, include bacteria (Staphylococcus aureus ATCC 6538P) 3339 mutant gram-positive bacteria, gram-negative bacteria is Escherichia coli (Escherichia coli ATCC 8739), Pseudomonas (Pseudomonas aeruginosa ATCC 9027), Candida albicans (Candida albicans ATCC 10231) recombinant yeast include, wetting ability (Aspergillus niger ATCC22343) Aspergillus age was used.

L applied on about 50 micro plate medium solidifying each strain for producing a flat medium dispensing a spreader (spreader) was uniformly using human herpesvirus. Said antibacterial composition in 10 mm diameter paper disk (paper disk) for absorption of about 40 micro l after dried and solvent medium is adhered on the surface strains of human herpesvirus 48 hours in culture nor post-paper disk formed in the vicinity of 30 °C condition extraction and measure the magnitude of syndromes (clear zone) transparent ring therefrom. The recording paper disk near microorganism growth are compensated by increasing average diameter value integer from 2 to 1,000. wide antimicrobial activity. In addition, DMSO (dimethyl sulfoxide) positive matching group, propanediol (propanediol, PD), methyl paraben (methyl paraben, MP) was the embodiment using the same method.

3. Steroidal anti-inflammatory test

In RAW 264 Mouse macrophage cell. 7 cell inflammation-inducing material to LPS (Lipopolysaccharide) is artificially after treatment inflammation billion number effect therefrom. 2 × 10 per well in 96 well plate⁵ Cell division so then, 24 hours 37 °C, 5% CO₂ Him as in incubator. Overnight replaced and fresh after LPS (1 ug/mL) which fit in each cosmetic composition prepared by the number in the embodiment and comparison to 24 by administering him as time according to concentration. Culture nor post-incubation, 13,000 rpm takes in only 3 minutes centrifuging the supernatant medium supernatant collecting Griess reagents with the 1:1 and reacting in absorbance 570 nm were measured.

4. Antioxidant active test

Removing free radicals ability to make sure that the anti-oxidant activity were measured. Removing free radicals at the side of a DPPH (1, 1 a-diphenyl-a 2 non-picryl-a hydrazyl) performed using [e tax it will reach when the, positive controls include L - ascorbic acid (L-a ascorbic acid) was used. Said DPPH 517 nm light in itself is very stable free radical as representing the absorption characteristic and violet compounds, that are stable to alcohol during protein - (proton-a radical scavenger) number radicals antioxidant mechanism since decolorized by synthetic antioxidant activity sensors can be monitored. In addition, IC₅₀ A stand-alone free radical formation of concentration required 50% number coupler is regulated by big.

5. Short moisturizing test

Antennae relative to the skin of a query by embodiment diagnosed and inspection device for skin, and selecting criteria is met, the air number name when the selecting not correspond it gave 10, applied to test until game evaluation to face sharing time 24 in skin lotion effect evaluation by dielectrophoresis.

Test site (before [wan department) left or right arm divide a calculator, or both arm and wrist 5 cm distance from pak before oyster side department site, each test site 1 cm now won of a 1. 5 cm intervals by me. Non-invasive measurement device using the equipment of the air conditioner conditions (temperature 20 °C, humidity 40%) in 30 minutes waiting subject prior value was waiting. 30 minutes after, test site (CM825, Courage & Khazaka, Germany) measuring 503,506 Coneometer to 5 times, a number of measurements from a terminal been external grudge maximum minimum 3. After evaluation device, test site in cosmetic composition prepared by the number in the embodiment and comparison to subject each then applied and sufficiently absorbed by 2 mg/cm2, so as to wait in air conditioner. Sample 30 minutes after application, 1 time, about 2 time, time 4, time and 24 points to each test site 6 same method were measured.

6. Cytotoxic test

Raw264. 7 B16/F10 cells on Korean cell line bank/10% fetal bovine serum and 1% penicillin-resistant Streptococcus pneumoniae (penicillin/streptomycin, GIBCO) receives a completed from Streptomyces (fetal bovine serum, GIBCO) erythromycin containing DMEM (Lonza) and adhesive 37 °C, 5% CO₂ Him as in a constant-temperature condition. The, cell toxicity MTT (3 - (4, 5 a-dimethylthiazol-a 2 a-yl) - 2, 5 a-diphenyltetrazolium bromide) method using conducting. Specifically, in which reacts with the enzyme of mitochondrial cells generated (DMSO) for 1 to 570 nm absorbance of formazan (Formazan) methyl opinion bombing death id die by measuring cell survival rates were measured.

7. Human concubine gun test

For discharging the composition for the skin-effect of the present invention assess sensitivity, predetermined test subject (in the embodiment 1 10 name, in the embodiment 2 10 name, comparison example 1 10 name and comparison example 2 10 name) 40 name randomly allocating used during each so as to output the main 4. Each of the test subject before use and use of carbostyril derivatives for main target 4, oil and moisture measurement was embodiment.

Skin erythema (Chromameter CR provided 300, Minolta, Japan) were measured using a color difference meter. Skin color difference meter measuring the colorizing, to L * a * b * 3 dimensional represented, a * value (negative value) and the green value (pasitive value) between double red erythema evaluation as follows. The, larger carbostyril derivatives for topology is a * value in spirits.

AC electrolysis tank is characterized skin sebum grader (Sebumeter SM810, C + K Electronic Co. , Germany) T zone sites using 1 times, 25 seconds were measured. , the higher the regenerating skin oil for producing paraformaldehyde in spirits.

Skin moisture moisture with mouse (Corneometer CM820, C + K Electronic Co. , Germany) was measured using ball sites utilizing average 3 times. , the higher the skin for regenerating a high moisture content and in spirits.

(In the embodiment 1)

Step 1. Number bath [sop[sop] extract

Ethanol 70% (V/V) aqueous solution of dry weight 100g pulverizing [sop[sop] 12 time heating reflux extracting, after liver leucorrhea needle grudge 1 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 5% by weight with respect to the so dissolved in distilled water.

Step 2. It buys elder brother anger extract number bath

100g of 70% (V/V) ethanol aqueous solution 12 dry weight of pulverizing it buys elder brother anger after extracting time heating reflux liver leucorrhea needle grudge 1, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 3% by weight with respect to the so dissolved in distilled water.

Step 3. Number of elder flower extract bath

100g of elder flower (V/V) ethanol aqueous solution of 70% dry weight of pulverizing liver leucorrhea needle grudge extracting time heating reflux after 1 12, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 25% by weight with respect to the so dissolved in distilled water.

Step 4. Number bath primrose extract

70% dry weight of the flowers and leaves (V/V) ethanol aqueous solution 12 100g pulverizing western candle warming reflux extracting time after liver leucorrhea needle grudge 1, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 5% by weight with respect to the so dissolved in distilled water.

Step 5. Poly ionic complexes (A) number of bath

1g been dissolving and mixing of water using the first 90g. Methods of ethanol obtained by dissolving 30 mg 10g after party [til ethanol amine, said resulting products mixed with a solution of 70 °C 2 in time with respect to the ethanol by distillation with stirring using the first heating.

Step 6. Number bath cosmetic composition

Said step 1 to step 4 in table 1 means in said step 5 each prepared by the number extract composition of cosmetic composition by mixing poly ionic complexes prepared by the number number was high pressure liquid coolant.

Said method using said method embodiment of the cosmetic composition prepared by the number manner to stability evaluation, shown in table 2 for the result. In addition, the cosmetic composition prepared by the number of said method using said method conch, effect is evaluated to, the table 3 to table 8 shown to result.

(In the embodiment 2)

Step 1. Poly ionic complexes (B) number of bath

300 mg of a 1, 3 - butylene glycol has been warmed to a ceramide DS provided Y30 10g 70 °C dissolving. The steps of 5 poly prepared by the number which can be ionic complexes in said in the embodiment 1, 2 hours in 70 °C agitating, the anionic phosphate physically carried - ceramide DS provided Y30 party [til[til] ethanol amine poly ionic complexes bonded fine particles number was high pressure liquid coolant.

Step 2. Number bath cosmetic composition

In the embodiment 1 in steps of 5 said step 1 is used instead of poly ion luck [chap body number under the outside ionic complexes poly prepared by the number and composition of table 1 was number cosmetic composition for mixing high pressure liquid coolant.

Said method using said method embodiment of the cosmetic composition prepared by the number manner to stability evaluation, shown in table 2 for the result. In addition, the cosmetic composition prepared by the number of said method using said method conch, effect is evaluated to, the table 3 to table 8 shown to result.

(In the embodiment 3 - 5)

The composition of table 1 was number cosmetic composition for mixing high pressure liquid coolant. Each manufacturing method of a plant extract present the disclosed.

Said method using said method embodiment of the cosmetic composition prepared by the number manner to stability evaluation, shown in table 2 for the result. In addition, the cosmetic composition prepared by the number of said method using said method conch, effect is evaluated to, the table 3 to table 8 shown to result.

Step 1. Mint extract number bath

100g of 80% methanol aqueous solution (V/V) pulverizing a dry weight of 12 time warming reflux extracting liver leucorrhea needle grudge putting after 1, 1. 2 micro m transmission filter to an upper was found. After methanol distilled and number and pressure alone, solids content 0. 2% by weight with respect to the so dissolved in distilled water.

Step 2. The low which it will carry on shoulder extract number bath

Pulverizing the low which it will carry on shoulder 12 a 100% dry weight 100g of structural formula 1 after liver leucorrhea needle grudge extracting ethanol from time heating reflux, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 15% by weight with respect to the so dissolved in distilled water.

Step 3. Extract of consonant number bath

100g pulverizing dry weight of 100% aqueous solution after 12 time heating reflux extracting liver leucorrhea needle grudge consonant seconds leaves 1, 1. 2 micro m transmission filter to an upper was found (solids content 0. 2% by weight).

Step 4. Speed well extract number bath

100g of 70% (V/V) ethanol aqueous solution 12 dry weight crushing speed well after extracting time heating reflux liver leucorrhea needle grudge 1, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 5% by weight with respect to the so dissolved in distilled water.

Step 5. Lemon balm extract number bath

70% (V/V) ethanol aqueous solution 12 100g pulverizing dry weight of lemon extract night time heating reflux after liver leucorrhea needle grudge 1, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 15% by weight with respect to the so dissolved in distilled water.

Step 6. Yarrowia shadow extract number bath

Ethanol 70% (V/V) aqueous solution of dry weight of yarrowia 100g pulverizing subtract 1 after extracting liver leucorrhea needle grudge 12 time heating reflux, 1. 2 micro m transmission filter to an upper was found. After ethanol distilled and number of decompressing the stand-alone, solids content 0. 15% by weight with respect to the so dissolved in distilled water.

(Comparison example 1)

Poly ionic complexes (A) and does not under the outside number using said in the embodiment 1, the composition of table 1 was number cosmetic composition for mixing high pressure liquid coolant.

(Comparison example 2)

It buys elder brother anger extract, elder flower extract, extract primrose, mint extract and 1% anionic solution by mixing the composition of cosmetic composition was high pressure liquid coolant to table 1 number.

(Comparison example 3)

[sop[sop] extract, elder flower extract, extract primrose, mint extract, extract the low which it will carry on shoulder, extract consonant, speed well extract, lemon balm extract and mixing cosmetic composition to the composition of table 1 yarrowia shadow extract number was high pressure liquid coolant.

The method of comparison example 1 to 3 said cosmetic composition said method of embodiment manner stability evaluation number produced therewith by, for table 2 have shown to result. In addition, the cosmetic composition prepared by the number of said method using said method conch, effect is evaluated to, the table 3 to table 8 shown to result.

Said table 2 as seen, the present invention according to comparison example contrast temperature storage-stable cosmetic composition were added instead of living. The active ingredient of a plant extract while different phenomenon can be collected in a specific number billion by precipitating the complex is suspended or crystal according to, this combined extract of the present invention poly ionic complexes by synergistic effect according to respective expected as follows.

Said table 3 as seen, the present invention according to aqueous cosmetic composition contains his car. In particular, an excellent antimicrobial activity were able to confirm that the charge sequence and Pseudomonas aeruginosa.

Said table 4 as seen, cosmetic composition the present invention according to positive controls contrast acid or a nitric oxide donor billion identifying number ability at the charge were. The, [sop[sop] extract, extract it buys elder brother anger, elder flower extract, extract primrose each when used alone, a nitric oxide donor billion number is employed for 30%, 21%, 11%, 8% corresponding to him. In addition, these extract 2 species combination also [sop[sop] extract employed for plant extract in nitric oxide donor billion number (30%) horizontality of the similar point viewed, the present invention according to the cosmetic composition for nitric oxide donor number molecular genetic methods in which disclosed can be employed billion.

Said table 5 as seen, cosmetic composition the present invention according to somewhat high IC rather than ascorbic acid₅₀ Value but, significantly improved charge removing free radicals ability to confirm that the comparison forecast disclosed were. In addition, the present invention according to cosmetic composition mint extract, extract the low which it will carry on shoulder, extract consonant, speed well extract, lemon balm extract, further comprises at least one plant extract selected from yarrowia shadow extract by, in the above-mentioned efficacy, effect that excels absorption spectrometry substrate.

Said table 6 as seen, the present invention according to 80% or more in both short-term moisturizing cosmetic composition represents been can be ascertained. This comparison example 2 including anionic contrast improved moisturizing force even when a corresponding to 56% or more, the present invention according to comparison example moisturizing cosmetic composition in which contrast effect force show other.

Said table 7 as seen, the present invention according to IC cosmetic composition₅₀ Value is 5. 0 sodium ocfosfate contrast 1000 times more regulated voice, 50 times maximum contrast comparison example having low cytotoxicity has been confirmed. I.e., the present invention according to cosmetic compositions have a good levels of toxicity to the cells by highly excellent in conductivity stability has been confirmed that the raw materials.

Said table 8 as seen, the present invention according to comparison example contrast carbostyril derivatives for cosmetic composition, a birth the oil and moisture as signal peptides. Therefrom, the present invention according to cosmetic composition when applied to the skin, skin erythema effective port, number is the oil secretion amount, increases moisture content, and cherry sensitive improvement pertinent data exhibit hif2e..