ANTI-AGING COMPOSITION FOR SKIN EXTERNAL APPLICATION COMPRISING MAGNOLIALES PLACENTA CULTURE EXTRACTS

Microware filld of processing for extracts of the present invention according to (Magnoliales) neck tylinoside

(1) cell separation

1.1 tylinoside neck (Magnoliales) [...] separation

Culture for, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, 70% ethanol and 5 minutes to 0.5% sodium hypochlorite for blossoms lifestyle month tree sterilization sanitizing, sterilization is washing several times number. Carefully forceps and peeling away the wall inedible using, including 5% Sucrose separated [...] MS medium (Duchefa, Cat# M0256) in good daytime 2-3.

1.2 tylinoside neck (Magnoliales) of separating flower

Culture for, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, 70% ethanol and 0.5% sodium hypochlorite to flower petal lifestyle month tree sterilization sanitizing 1 minutes, sterilization is washing several times number. Carefully pincette and using sharp knife to cut a wafer adhered with a plate is provided to perform thermal, device, a flower portion is 5% Sucrose MS medium including (Duchefa, Cat# M0256) by of intermeshing is to good daytime 2-3.

1.3 of separating leaves (Magnoliales) neck tylinoside

Culture for, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, lifestyle month tree leaves 0.5% sodium hypochlorite to 70% ethanol and sterilization sanitizing 1 minutes, sterilization is washing several times number. Carefully pincette and using sharp knife to cut a wafer adhered with a plate is provided to perform thermal, device, a medium including 5% Sucrose portion leaves MS (Duchefa, Cat# M0256) by of intermeshing is to good daytime 2-3.

1.4 of separating (Magnoliales) neck tylinoside

Culture for, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, lifestyle month tree leaves 0.5% sodium hypochlorite to 70% ethanol and sterilization sanitizing 1 minutes, sterilization is washing several times number. Carefully pincette and using sharp knife to cut a wafer adhered with a plate is provided to perform thermal, device, a MS medium including 5% Sucrose is trunk portion (Duchefa, Cat# M0256) by of intermeshing is to good daytime 2-3.

1.5 (Magnoliales) of separating root neck tylinoside

Culture for, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, lifestyle month tree leaves 0.5% sodium hypochlorite to 70% ethanol and sterilization sanitizing 1 minutes, sterilization is washing several times number. Carefully pincette and using sharp knife to cut a wafer adhered with a plate is provided to perform thermal, device, a root portion including 5% Sucrose MS medium (Duchefa, Cat# M0256) by of intermeshing is to good daytime 2-3.

(2) separated tylinoside neck (Magnoliales) [...] , flower, leaf, stem and roots culture of cells and mass production

Sterile separated tylinoside neck (Magnoliales) [...] , flower, leaf, stem and roots cells including 5% Sucrose MS manner in a medium free of the n bit parallel data inputted sebocytes are excellently interval 2-3 for a certain period of time with attention, after, bioreactor having balloon (ternary science) Agar except for using an identical composition 25 temperature in a liquid culture medium, and offensive odor during the interval 0.1vvm leakage of oil to the constant throughout the growth of the main 14 as early as the culture of proliferating mass.

(3) (Magnoliales) neck tylinoside [...] , flower, leaf, stem and roots cell culture of processing for extracts of

(Magnoliales) which are representative of a neck tylinoside produced from the plant seed, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, lifestyle month tree[...] , flower, leaf, stem and roots cells harvesting is lower than the tissue is sufficiently removes moisture of 2 to 60 °C during dryer at was very dry. [...] of (Magnoliales) neck tylinoside dried, flower, leaf, stem and roots cells the support the container 100g powder, purified water 10 L is injected into the medal and then, 8-48 time in distiller scalding, heated to 80-100 ° C hot-water extract is obtained. After extraction, mesh are filtered to remove the solids, of an, tylinoside neck (Magnoliales) which are representative of a, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, lifestyle month tree cell culture extract manufacturing processes and the cost of production.

Experiment 1 e.g.: neck tylinoside the present invention according to (Magnoliales) microware filld cell culture extract test be achieved, wherein an influence on cell survival rate

Microware filld neck tylinoside the present invention according to (Magnoliales) to the cells of the skin culture extract cells proliferation of the to see activity, human keratinocyte forming cells (Human Skin Keratinocyte, HaCaT) good receiving in the ATCC (American Type Culture Collection). Cells 1×10⁴ cells/ml on the concentration of was to be inoculated with a 24 well culture plate. Media containing 10% FBS DMEM (Dubelcco ' S Modified Eagle Medium, BRL, USA) by cylinder to have. 10% FBS in DMEM containing 48 time by culturing cultured by 25-30% surface area of the culture vessel, the surface, in the embodiment 1 (Magnoliales) neck tylinoside made in cell culture extract [...] by replacing a FBS-free DMEM containing 10% 24 hours' extra good. Culture nor post-incubation 3-(4, 5-aminothiazole dimethyl-2-one)-2, 5-diphenyl tetra thiazolium bromide (MTT, Sigma M5655, USA) solution (2.5 mg/ml) for 50 micro l 3 adding further time to remove the supernatant after grown on, each well of 200 per Dimethylsulfoxide (DMSO, Sigma D2650, USA) solution after loading the formazan formed agitating (formazan) 20 minutes then, determination, 100 micro l of 570 nm to take 96 well it was determined that the absorbance in Enzyme-Linked Immunosorbent Assay (ELISA). Degree of proliferation activity of skin cells using pure water the control group of absorption strength reference so as to provide expressions as calculated according to the percentage layer containing a filler.

[Expressions 1]

Cell survival rate (%) = effect (Viability) (extract of the absorption edge of the group/collating absorbance in processing) 100

Human keratinocyte forming cells using (keratinocyte), tylinoside neck (Magnoliales) representative plant by the compressive force of the cell culture extract and suitable writing the skin results see ranges, i.e., Cell Viability cell survival rate effect identified. 1% concentration of tylinoside neck microware filld when cell culture extract blended, companion does (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, microware filld cell culture extract lifestyle month tree do not affect the cell viability identifying improving.

Experiment 2 e.g.: test and concentration component confirmation by extract

The present purpose of the invention belonging to the tylinoside neck (Magnoliales) cell microware filld plants that produced from the plant seed the proliferation by culturing microware filld polyphenol compound which are the product of secondary metabolic in cells in order to achieve a high content (polyphenolic compounds) physical and chemical attractant by using number (elicitor).

Tylinoside neck (Magnoliales) belonging to secondary metabolic is displayed on a right box plant cells which are the product of carotenoid, biflavonoid and phenolic logic state by comparing the content of the method

1 . 200 M methyl jasmonic acid (methyl jasmonic acid) for MS packed in culture the medium.

2. Tylinoside neck (Magnoliales) a plant belonging to the high frequency embryoid bodies cells microware filld in independently, making various experimental conditions while day high frequency interval ingredient 5 on the 270kHz or 240 3 burn for week or other migration was repeated days.

Neck tylinoside by the present invention (Magnoliales) during a majority component of culture extract cells microware filld antioxidant effect associated with phenolic material, for heterologous total content and total flavonoid content together with the information to make sure that is performed for all the experiments.

Total content reading

Distilled water to 1 extract after is added is 10 ml, 2 ml of adding Folin-Ciocalteu phenol reagent (Sigma) then an, 5 reacted minutes at room temperature. 20% sodium carbonate reactants is added 2 ml then an, 1 time at room temperature then the microplate reader (UVT-06685, Thermo max, USA) using it was determined that in 680 nm absorbance. The, effective ingredient the gallic acid (gallic acid, Sigma, USA) jet stream by using a carrier gas, index made curve assay by concentration of the substance of the absorption edge of the each sample showed to 2 the table large-particle-size-side value.

Total flavonoid content reading

Tylinoside neck (Magnoliales) 1.5 ml culture extract cells microware filld dissolved in methanol the composition in an equal amount and to 2% AlCl36H2O 10 minutes at room temperature then mixing reacting a metal salt of the microplate reader (UVT-06685, Thermo max, USA) 367 nm it was determined that in using absorbance. The effective ingredient (catechin, Sigma, USA) and catechin jet stream by using a carrier gas, index made curve assay by concentration of the substance of the absorption edge of the each sample showed to 2 the table large-particle-size-side value.

Said table 2 during neck tylinoside result of MeJA (methyl jasmonic acid) attracting does companion subjecting the film to a treatment by number (Annona squamosa) is LED to cell culture extract microware filld in polyphenol content, been representing high flavonoid content, subjecting the film to a treatment by number attracting Radiofrequency right gun E mote Ohus or (Eupomatia laurina), Mickle neck, tulip tree, tree box Pak flower , microware filld lifestyle month tree in cell culture is LED to high extract showed flavonoid content content and phenol. Furthermore, tylinoside, in the case of wood schizandra, attracting agent the highest memory state in untreated [...] polyphenol content, have shown that flavonoid content.

Experiment 3 e.g.: tylinoside neck [...]antioxidant effect (RadicalScavengingActivityABTS)

Using ABTS radical antioxidant potency measurements generated by reaction with potassium persulfate ABTS free radical in the extract antioxidant index of unique radical is removed by reflowing the solder and a depigmenting is resolution radar Image using the method such as Van den Berg in it was determined that adjusted by deforming the method.

1.0 mm of AAPH (2, 2 '-azo-bis 2-amidinopropanedeihydrochloride) senses a rotation velocity of the disk to the 100 mm PBS buffer 2.5 mm ABTS (2, 2' -azino-bis 3-ethylbenzenothiazolin-6-sulfonic acid) and a after mixing in thermostat of 68 °C 12 in the embodiment 1.1% concentration reacted minutes extract of 980 and a l micro 20 micro l ABTS solution 10 minutes in 37 °C water bath that decreases from the 734 nm while reacting extinction a measure of the degree to which the control group positive the corresponding advertisement based on the shown by cylinder to have the Trolox 0.1%. ABTS radical erase royal tomb then corresponding advertisement based on the shown list as calculated according to 2 of equations, tables result as and a 3.

[Expressions 2]

Said collected and may be found in table 3, microware filld neck tylinoside concentration of 1% composition of the present invention during [...] other than culture extract induced at the sites where best the antioxidant ability 80 M. has been confirmed.

Experiment 4 : B16F10 malanoma e.g. measuring melanogenesis using cellinhibitors

A producing cells of B16F10 Melanin 10% FBS, 1% antibiotics added at a water 37 °C manner in a medium free of DMEM, 5%, CO2 [...] neck tylinoside of. 1% good conditions by B16F10 cell for generation of a melanin to confirming change 1.5×10³ cells/well including DMEM medium at a concentration of visitor is checked through a suspended in a 10% FBS. Suspension cells (5 ml) after placed in a tissue culture flask attached to between 1. After assay after which the sample is added, good between 5 37 °C conditions in 5% CO2. Culture is B16F10 cell trypsinization the washing to a phosphate buffered saline (PBS). Cells after is gathered to corning tube 1×10⁶ cell/1N NaOH per 1 ml solution then, cells extended storage and is easily carried, measures the absorbance microplate reader to 490 nm in melanogenesis was is obtained rate inhibitors.

[Expressions 3]

Inhibition of melanogenesis (%) = [1-(Absample/Abcontrol)] × 100

Neck tylinoside in the present invention of B16/F10 melanoma cell extract cell culture microware filld of plants may be achieved, wherein an influence synthesis to make sure that, therefor are subjected to treatment with 1% concentration between 3 grown on melanin total after it was determined that amount. As a result, 1% tylinoside neck of plants a whitening effect by cell culture microware filld [...] only the n bit parallel data inputted confirm that the user reducing melanin synthesis, remaining portion of the in a shaking the whitening effect.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting microware filld cultured by processing number in the case of cells, are not to be treated are agent attracting [...] amount extract 2 times than if the been shown that reducing melanin synthesis, 1.2 1300 the other parts cell culture extract showed reducing the melanin synthesis.

Experiment 5 e.g.: Procollagen synthetic enhancing test

37 °C a human fibroblast (Human Skin Fibroblast), 5% CO2 incubator in a substantially constant in independently, making various experimental conditions maintaining the humidity of 10% FBS, Penicillin (50U/ml), Streptomycin (50/ml) containing DMEM (Dulbecco 's Modified Eagle' s Medium, Gibco, USA) the culture the, 1×10⁵ 24 well plate to open/ml to 500 micro l at 24 then divides the good time. The control group (DMEM medium) and 1% of concentration of changable plant neck tylinoside obtained at the in the embodiment 1 cell culture extract 48 for inserting and removing a broth medium containing rice 37 °C time, good 5% CO2 incubator in. 48 process has been completed, each the supernatant medium takes an 20 micro l Procollagen Type I C-Peptide EIA Kit (PICP, Takara, Cat No. MK101) by measuring the contact it was determined that amount collagen synthesized de novo. The amount PICP ng/1×10⁵ cells in terms of a product having a corresponding advertisement based on the shown list, as calculated according to expressions to tables result to the computer of the 5. shown in an.

[Expressions 4]

Understructure may be found in said table 5, during microware filld plant neck tylinoside concentration of 1% in the embodiment 1 by treating by [...] Procollagen biosynthesis S70 significantly increasing confirm that the user has been, even not remaining extraction site also [...] is attached on the circumference for a difference from an growth promoting effect collagen synthesis is displayed on a right box can be viewed.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting microware filld cultured by processing number in the case of cells, are not to be treated are agent attracting extract amount [...] than if the exhibit increased 2.5 times Procollagen been S70 biosynthesis, the other parts cell culture extract approximately 1.2 1300 Procollagen revealed a increased S70 biosynthesis.

Experiment 6 e.g.: diseases by inhibiting active iNOS clause salt effect experiment

Raw 264.7 cell line produced from the amount of nitric oxide (NO) NO2-form present in the cell culture by using Griess reagent was in. 1g LPS-activated Raw 264.7 cell to measure of inhibiting the formation NO, microware filld plant neck tylinoside concentration of 1% cell culture extract and 24 for the control group group the cells to a type used in cell culture time after the cell culture supernatant Griess reagent 100 micro l and a Griess reagent (1% sulfanilamide in 5% phosphoric acid, 1% -naphthylamide in H2O) 96 well plates in 100 micro l by mixing the reacting a metal salt of 540 nm in 10 minutes it was determined that absorbance. NO2-the concentration of the sodium nitrate dilution measures the absorbance a standard curve is obtained.

Such as said table 6, the activity of the iNOS microware filld plant neck tylinoside concentration of 1% during subjecting the film to a treatment by [...] NO the control group compared to the best inhibits production using the inhibitors of inflammation.

Furthermore, high frequency zero attracting in independently, making various experimental conditions 240-270 kHz high frequency day while 5 to 3 burn processing interval ingredient, the migration same employ repetitious treatments days in the case of cells cultured microware filld, [...] amount extract attracting agent than if the are not to be treated are 1.8 times iNOS been that is active, the other parts the shaking adding water, raw milk, culture extract cells.

Experiment 7 e.g.: skin moisturizing efficacy experiment

1. Companion does (Annona squamosa)

2. right gun E mote Ohus or (Eupomatia laurina)

3. Tylinoside

4. Mickle neck

5. Tulip tree

6. Wood box Pak flower

7. Wood schizandra

8. lifestyle month tree

1 to see the speed skin moisturizing when applied times, or lake drying, dry skin as remaining adult to 30-40 segments in the sequence of, proposing 50 to said face and of after between the space filled with mortar applied to inner pak before , 24 five meters[...] time (corneometer; CM820 courage Khazaka electronic GmbH, Germany) skin using it was determined that amount of water. [...]five meters present in skin's skin by using the complex sensor structure amount of moisture and a preparation method ion of moisture the amount of moisture input sound by calculating a which determining the moisturizing, measuring method to equal.

1) skin site to be measured overall raised the prices probe five meters[...] to.

2) probe to the skin, the upper part of the key when the electric conductivity of the skin sensor into a number (capacitance) been the mobile telephone.

3) prints an measurement site, measurement was repeated.

4) once when measuring, after wiping the sensor at a resting such [...] , it was determined that again.

Constant temperature before starting of pre-applying in said method, lighter conditions (24 °C, humidity 40%)in skin using five meters[...] measures the and as a basic value of the moisture amount of the, 12 time, it was determined that 24 change at the passage of time. The result is a to table showed to 15.

From the results of said, non 1-8 memorable friendship and 12 when the is applied to the skin after application the moisture amount of the device after a lapse time but increases to some extent, before applying the device after a lapse time 24 and returns into a state, in which the can be viewed. While, does companion plant representative neck tylinoside concentration 1% (Annona squamosa), (Eupomatia laurina) right gun E mote Ohus or , tylinoside, Mickle neck, tulip tree, box Pak flower wood, wood schizandra, microware filld lifestyle month tree composition for skin external application containing culture extract cells number manufacturing composition 8-5 e.g.-producing 1-1 e.g. when is applied to the skin, the, 24 force moisturizing the same skin is time or more memorable friendship and non in addition increased moisture content higher than 1-8 could confirm it.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting in independently, making various experimental conditions and high frequency processing number while 240-270 kHz high frequency day 5 to 3 burn processing interval ingredient, migration same microware filld cultured the employ repetitious treatments days in the case of cells, are not to be treated are [...] amount extract attracting agent 1.5 times better than if the skin moisture increase been visible, the other parts cell culture extract also 1.1 times using the skin moisture increase.

Experiment 8 e.g. :, contraction of pores effect test (In Test vitro)

, Contraction of pores composition of the present invention for determining effects said in the embodiment the subject compositions made in the skin replace the protein using haemoglobins. 0.9% Phosphate Buffer Saline was test the effects, contraction of pores (0.1 mm, pH 7.4) in a hemoglobin solution (0.05g/50 ml) 2 ml to a hemoglobin solution thereby significantly decreasing concentration of glucose, said in the embodiment compositions obtained in 2 ml after loading the, 30 seconds when blended with an agitated in 3,500 rpm and centrifugation 10 minutes, 2 ml purified water to 1 ml supernatant thereof 407 nm is applied to in factor have been measured by UV-Visible spectrum. The control group in said embodiment the first deoxygenator applying PBS (Phosphate Buffer Saline) 2 ml was instead composition. Mixtures of varying concentrations when groups the experiments composition, hemoglobin case of a sudden brake, the means of a needle electrode of the n bit parallel data inputted estimate the antimicrobial effect shrinkage, (%) precipitation of hemoglobin, contraction of pores high the determined to play effect; a process for manufacturing the.

Hemoglobin case of a sudden brake, the means of a needle electrode of the n bit parallel data inputted estimate the antimicrobial effect shrinkage, (%) precipitation of hemoglobin, contraction of pores high the determined to play effect; a process for manufacturing the. As seen from the results of table 16, microware filld plant neck tylinoside 1% cell culture extract a significant show differences which, showed efficacy, contraction of pores.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting in independently, making various experimental conditions and high frequency processing number while 240-270 kHz high frequency day 5 to 3 burn processing interval ingredient, migration same microware filld cultured the employ repetitious treatments days in the case of cells, are not to be treated are agent attracting extract amount [...] than if the 2.2 times better, contraction of pores using the been, 1.1 times the other parts cell culture extract also increased, contraction of pores using the.

Experiment 9 e.g.: pin experiment inhibitory effect

PC 10 name [...] of the present invention composition using culture extract cells microware filld plant-derived neck tylinoside 1% 4 week or two skin oil measuring instrument (Sebum meter SM810) by sebum secretion of skin using it was determined that amount. 22±2°C experiment, 40±5% humidity in been made. Oily skin if the measured value is at least 220 micro g/cm2, when the normal skin is 100-220 micro g/cm2. The n bit parallel data inputted using the control group purified, experiments as tables 17.

From the results of said table 17 as seen from, the control group compared to neck tylinoside 1% amount [...] of plants by sebum secretion off the best by using the inhibitory effect.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting in independently, making various experimental conditions and high frequency processing number while 240-270 kHz high frequency day 5 to 3 burn processing interval ingredient, migration same microware filld cultured the employ repetitious treatments days in the case of cells, are not to be treated are agent attracting extract amount [...] than if the 2.5 times better by sebum secretion inhibitors been using the, the other parts cell culture extract also 1.3 by sebum secretion increased times using the inhibitory effect.

Experiment 10 e.g.: laminate for printed wiring produced

Neck tylinoside 1% composition of the present invention of the present invention as a beverage for amount [...] of plants resulting from tests on assay laminate for printed wiring produced effect that produces an anti-microbial testing solid cultivation dilution by the embodiment. To enable the implying bio-technology Institute from protease, its strain experiment, Staphylococcal in gram-positive bacteria, (Staphylococcus aureus KCTC 6910), (Pseudomonas aeruginosa KCTC 1637) Pseudomonas aeruginosa by gram-negative bacteria, e. coli (E. Coli KCTC 1039), (Candida albicans KCTC 7965) yeast Candida yeast is usually called as red yeast, filamentous fungus strain species 4 (Aspergillus nigerKCTC 6910) black country germ in the first voice portion out of an total or more.

Solid cultivation dilution method (Agar Serial Dilution Method)

Bacteria the n bit parallel data inputted media for SOI static a tripping element, the culture medium to which bacteria in incubator succussion occurs 37 °C thereby, the third to eo by amount pre-oriented time 24. Yeast culture the potato dextrose media for thereby bacteria the culture medium to which the n bit parallel data inputted in incubator succussion occurs 25 °C, the third to eo by amount pre-oriented between 2. Filamentous fungus culture of the potato polydextrose agar media for thereby bacteria the culture medium to which the n bit parallel data inputted in incubator 25 °C, the third to eo by amount pre-oriented between 7.

First test apparatus antimicrobial more particularly in the case of bacteria, thereby bacteria the culture medium to which SOI static trip 37 °C in preparing and by amount pre-oriented time 24, in the case of yeast, potato dextrose the culture medium to which bacteria in 25 °C thereby wherein amount pre-oriented between 2, filamentous fungus the potato dextrose agar the culture medium to which bacteria in incubator 25 °C thereby after both grudges pre-oriented between 7, using electrod smears, medium formed on the surface the filamentous fungus of spores for in the sterilized saline to the supernatant may be processed to recover by dilution. In the sterilized plaque tree according comprises 306 BP of the 5% DMSO (Dimethylsulfoxide) and sample for producing dynamic ram suitable physiological saline solution, or have inadequate concentrations of these 2 ml extract doesn't have any error frames, at each dilution, the control group the 5% physiological saline solution, or have inadequate concentrations of these suitable 2 ml each sample doesn't have any error frames, at a dilution, the control group the physiological saline solution after added of 2 ml 5% DMSO, sterile and each plaque tree for producing dynamic ram cooled to 48 °C trip tic incendiary polydextrose potato medium and 18 ml agar agar medium after the at was political and solidify the filaments. Amount pre-oriented after each trial which has been about final concentration in the case of bacteria strain of Aureobasidium 1 × 10⁶ CFU/of concentration each PET [...] smearing, in the case of yeast 1 × 10⁵ CFU /, if of filamentous fungus 1 × 10⁴ CFU/ [...] PET each concentration of the smears. Each petri dish in a bacterium is 37 °C 24 3 25 °C in a yeast culturing the cell line in the time between culture, the filamentous fungus in incubator 25 °C between 7 into a compartment of a then grown on whether forming [...] to prevent a growth are observed to concentration sample minimum flat minimum inhibitory concentration (MIC, Minimum inhibitory concentration) which, showed to result to the computer of the table 18. The, the smaller the minimum inhibitory concentration a value means a having a high antibacterial effect.

Table 18 as demonstrated, microware filld plant-derived representative neck tylinoside 1% cell culture extract both been exhibit antibacterial effect, in particular, [...] laminate for printed wiring produced by silica is again coated with titanate system culture extract cells showed efficacy.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting in independently, making various experimental conditions and high frequency processing number while 240-270 kHz high frequency day 5 to 3 burn processing interval ingredient, migration same microware filld cultured the employ repetitious treatments days in the case of cells, are not to be treated are agent attracting extract amount [...] than if the exhibit antibacterial effect better times 1.5 been, 1.1 times the other parts cell culture extract also increased antimicrobial using the.

Experiment 11 e.g.: and an improvement effect of a acne

Acne 10 both sexes of the tread is generated for the subject to be name which have face lotion morning, daytime 4 night to bar ultrasonic waves to the entire face. Acne and an improvement effect of a participating experiments determination of identification number, according to a degree of feel with reference to a CDMA a state before test point 1-3 acne healing effect then result to the computer of the evaluate them showed listed in tables so that individual asset.

<Test before for evaluation of cancer, cancer >

Little negative acne 1 = 2 = 3 = be slightly acne by severe acne

<Acne effect determining >

-: Effect substantially no, + : some effect sound, ++ : much mitigating, +++ : completely healing is

Understructure is found in 19 of tables, via said, face lotion containing flexible [...] tylinoside 2% after it is used in daytime 4 majority subject of acne can be identifying improvement effects of a.

Furthermore, 200 M methyl jasmonic acid (methyl jasmonic acid) attracting in independently, making various experimental conditions and high frequency processing number while 240-270 kHz high frequency day 5 to 3 burn processing interval ingredient, migration same microware filld cultured the employ repetitious treatments days in the case of cells, are not to be treated are agent attracting [...] amount extract 2 times better than if the been exhibit antibacterial effect, 1.5 times the other parts cell culture extract also improved using the pimple increased.

Preparation of cosmetic composition

[...] neck tylinoside of the present invention useful as anti-cell culture in cosmetic containing the nutritional wash containing a crude drug, cream, seat belt such as cosmetic and belonging to dicksoniaceae of the dosage form emulsion such as cosmetic formulations such that the solubilization of the manufacturing processes and the cost of production.

Manufacturing e.g. 2-1: wash containing a crude drug

Then is useful have been prepared according to conventional wash containing a crude drug manufacturing method.

Manufacturing e.g. 2-2: seat belt

Then seat belt of typically result regimens have been prepared according to manufacturing method.

Manufacturing e.g. 2-3: lotion

Then lotion typically result regimens have been prepared according to manufacturing method.

Manufacturing e.g. 2-4: cream

Then approval typically result regimens have been prepared according to manufacturing method.

Manufacturing e.g. 2-5: gel

Then is useful have been prepared according to conventional gel manufacturing method.

At least specific content of the present invention described in detail a portion 360degree, homogeneously distributed to be person with skill in the art, such a preferred embodiment only the specifically and the user makes a is aspect, of the present invention the not range is limited will the apparent. Therefore, substantial of the present invention by issuing an ranges are defined by claim and their equivalent will the pixels include.